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At least 433 records · Page 24

On-Orbit OCI Characterization Measurements from the first 6 Months of the PACE Mission

The Ocean Color Instrument (OCI) on NASA’s Plankton, Aerosol, Cloud, and ocean Ecosystem (PACE) mission has been providing data to the science community since April 2024. OCI is a hyperspectral imager, providing almost daily global coverage, at a spatial resolution of 1.2km. Its design specifications were optimized for ocean color and atmospheric applications, but terrestrial studies could benefit from its hyperspectral coverage as well. The ocean color requirements called for very high radiometric accuracy, which could benefit a wide variety of applications. This paper presents results from the first 6 months of on-orbit calibration and characterization measurements, including absolute calibration, spectral registration, temporal trending of radiometric sensitivity, signal to noise ratio, and linearity, with a focus on the commissioning results obtained in the first 2 months after launch.

calibration↗

Pre-Launch Analysis and Test of the Ocean Color Instrument Modulation Transfer Function

Launched in February 2024, the Plankton, Aerosol, Cloud, and ocean Ecosystem (PACE) mission represents NASA’s next investment in ocean biology, clouds, and aerosol data records. The Ocean Color Instrument (OCI) is the primary instrument supporting PACE by collecting accurate radiometric data of the Earth by observing the top of atmosphere reflectance and is a combination hyperspectral (ultra-violet to near infrared) / multiband imager (shortwave infrared). An important aspect of the radiometry is the ability of OCI to resolve contrast from scene to scene to achieve data accuracy requirements. This paper reviews the analysis that was performed to verify the modulation transfer function requirement levied against the instrument and presents the supportive test data collected during pre-launch instrument testing to supplement the analysis. The requirements levied against OCI drove to a 1.2 km2 ground resolution leading to a Nyquist period of 2.4 km (0.417 cycles / km). The analysis is comprehensive with incorporation of as many contributors as practical to provide the clearest possible picture of the margin against requirements. The analysis provided input for additional design trade studies and insight into the aspects of the design that required the most attention during the implementation phase. We describe four separate MTF contributor networks based on the instrument design and mutual exclusivity of contributing factors. The various networks represent the hyperspectral and multiband detection systems separately as well as the along track and cross track imaging dimensions. Ground-test was performed against the MTF primary contributors and the results were used to validate the analysis as will be presented here.

OCI↗

OCI Geolocation Evaluation and Refinement Using Landsat Control Points

The Plankton, Aerosol, Cloud, and ocean Ecosystem (PACE) mission is NASA’s next investment in ocean biology, clouds, and aerosol data records. A key feature of PACE is the inclusion of an advanced satellite radiometer, Ocean Color Instrument (OCI), a global mapping radiometer that combines multispectral and hyperspectral remote sensing. The geolocation processing is performed for OCI using spacecraft navigation data and an instrument geometry model. To evaluate the geolocation accuracy for OCI and develop refinements to the processing methods, control point matching using Landsat data has been implemented as a step in the operational processing of OCI data at the Science Data Segment. This processing provides between 200 and 300 high-quality matchups per day with good global and geometric distribution, allowing rapid evaluation of the OCI geolocation accuracy. The results provided an early indication of the overall quality of the geolocation processing and of specific aspects needing improvement. A standard set of granules was identified to support rapid implementation and testing of geolocation refinements, and this approach has been highly successful in improving the geolocation processing accuracy to meet the science requirements. The evaluation will continue throughout the mission to ensure the ongoing accuracy of geolocation. This paper describes the control point matching methodology, the approach to development of the geolocation processing refinements, and the recent results.

PACE↗

PACE Microphysical Aerosol Properties from Polarimetry (PACE-MAPP)

We present the Plankton, Aerosols, Clouds and Ecosystems Microphysical Aerosol Properties from Polarimetry (PACE-MAPP) polarimetric remote sensing algorithm developed for the newly launched NASA PACE satellite observing system. The objective of PACE-MAPP is to retrieve detailed fine-mode (marine, pollution and smoke) and coarse-mode (sea-salt and dust) aerosol properties over the ocean for a range of light to heavy aerosol loadings using PACE’s polarimetric-imaging capabilities at multiple angles and wavelengths from the ultraviolet (UV) to the near-infrared (NIR). An additional objective for PACE-MAPP is to discriminate aerosols from thin clouds. The PACE-MAPP polarimetric remote sensing retrieval algorithm uses coupled atmosphere-ocean vector radiative transfer, optimal estimation, artificial intelligence and powerful inherent optical property look-up-tables for the Earth’s aerosol, cloud, and hydrosol particles. PACE-MAPP is the only retrieval algorithm designed to invert aerosol/ocean products using both polarimeters onboard PACE. We present results using PACE-MAPP to retrieve aerosol and ocean remote sensing products from measurements by the Hyper-Angular Rainbow Polarimeter #2 (HARP2) and Spectro-polarimeter for Planetary Exploration one (SPEXone) polarimeter instruments onboard PACE.

Snorre Stamnes↗

PACE OCI Straylight and Crosstalk Evaluation using Moon

The Ocean Color Instrument (OCI) onboard NASA’s Plankton, Aerosol, Cloud, ocean Ecosystem (PACE) mission has performed three monthly lunar calibrations. The moon is an extended source over a large dark background, making it an ideal target for evaluating OCI’s straylight and crosstalk performance. The lunar data analysis showed the straylight and crosstalk to be lower than the prelaunch measurements, especially in the along-track direction, where very little straylight is detected. Based on lunar data, the prelaunch measured crosstalk coefficients were reduced, and a crosstalk correction was tested on both lunar and solar calibration data. Applying the revised crosstalk correction, the crosstalk contaminations are significantly reduced to under 0.1% at 2-3 pixels away from the lunar boundary for bands above 350 nm. Below 350 nm, the crosstalk correction residuals gradually increase due to a lack of high-quality prelaunch measurements. Lastly, applying the crosstalk correction changes the calibrated radiance for all science data. This is due to the different spectral shapes between the solar diffuser and the observed scenes. For the moon, the crosstalk correction has an impact of ~0.4% on the overall calibrated radiance for the 400 – 600 nm bands and up to 20% impact on the UV bands.

Shihyan Lee↗

Attitude Ground System: Recent Experiences in Attitude Sensor Calibration and Upcoming Mission Support for Momentum Unloading

A key component of the mission ground system used for supporting NASA Goddard Space Flight Center space missions, known as the Attitude Ground System (AGS), is used to perform operational and analytical support for the spacecraft attitude con-trol system (ACS). The AGS is comprised of a real-time attitude analysis and moni-toring system, and a tool for performing offline analysis of the ACS system using key sensor telemetry collected and stored onboard and transmitted to the mission operations center. The AGS performs numerous functions, including independent ground attitude determination, attitude slew planning and commanding, attitude sensor calibration, and real-time monitoring of the health and safety of the ACS. The AGS is developed in MATLAB using a core library of utility functions called the Multi-Mission Three-Axis Satellite System (MTASS). The MTASS library has been used to support mission operations for over thirty NASA missions starting in the late 1990s. It is customized for each mission to meet specific mission requirements. In this paper we describe recent operational experience using the AGS to support the launch and early orbit (L&EO) phase of the Plankton, Aerosol, Cloud Ocean Ecosys-tem (PACE) and Joint Polar Satellite System (JPSS) missions. Our summary of L&EO support for PACE and JPSS includes ACS sensor calibration of the alignment and scale factors for the Star Trackers, Inertial Reference Unit, and Three-Axis Magnetometers. We will also describe new capabilities for planning and executing reaction wheel momentum unloading for the Roman Space Telescope (RST) During the spacecraft early orbit phase, the AGS is operated in the mission operations center by a team of subject matter experts that also develop and configure the AGS soft-ware. This unique approach, integrating system development and operation in a sin-gle engineering team, provides valuable expertise for quick assessment of ACS per-formance and safety; critical to anomaly resolution and mission success.

Guidance, Navigation, and Control↗

3D Cloud Masking Across A Broad Swath Using Multi-Angle Polarimetry and Deep Learning

Understanding the 3-dimensional structure of clouds is of crucial importance to modeling our changing climate. Active sensors, such as radar and lidar, provide accurate vertical cloud profiles, but are mostly restricted to along-track sampling. Passive sensors can capture a wide swath, but struggle to see beneath cloud tops. In essence, both types of products are restricted to two dimensions: as a cross-section in the active case, and an image in the passive case. However, multi-angle sensor configurations contain implicit information about 3D structure, due to parallax and atmospheric path differences. Extracting that implicit information can be challenging, requiring computationally expensive radiative transfer techniques. Machine learning, as an alternative, may be able to capture some of the complexity of a full 3D radiative transfer solution with significantly less computational expense. In this work, we make three contributions towards understanding 3D cloud structure from multi-angle polarimetry. First, we introduce a large-scale, publicly available dataset that fuses existing cloud products into a format more amenable to machine learning. This dataset treats multi-angle polarimetry as an input, and radar-based vertical cloud profiles as an output. Second, we describe and evaluate strong baseline machine learning models based that predict these profiles from the passive imagery. Notably, these models are trained only on center-swath labels, but can predict cloud profiles over the entire passive imagery swath. Third, we leverage the information-theoretic nature of machine learning to draw conclusions about the relative utility of various sensor configurations, including spectral channels, viewing angles, and polarimetry. These findings have implications for Earth-observing missions such as NASA’s Plankton, Aerosol, Cloud-ocean Ecosystem (PACE) and Atmosphere Observing System (AOS) missions, as well as in informing future applications of computer vision to atmospheric remote sensing.

clouds↗

Far and Wide - Microbial Bebop

This musical composition was created from data of microbes (bacteria, algae and other microorganisms) sampled in the English Channel. Argonne National Laboratory biologist Peter Larsen created the songs as a unique way to present and comprehend large datasets. Microbial species of the Order Rickettsiales, such as the highly abundant, free-living planktonic species Pelagibacter ubique, are typical highly abundant taxa in L4 Station data. Its relative abundance in the microbial community at L4 Station follows a distinctive seasonal pattern. In this composition, there are two chords per measure, generated from photosynthetically active radiation measurements and temperature. The melody of each measure is six notes that describe the relative abundance of the Order Rickettsiales. The first note of each measure is from the relative abundance at a time point. The next five notes of a measure follow one of the following patterns: a continuous rise in pitch, a continuous drop in pitch, a rise then drop in pitch, or a drop then rise in pitch. These patterns are matched to the relative abundance of Rickettsiales at the given time point, relative to the previous and subsequent time points. The pattern of notes in a measure is mapped to the relative abundance of Rickettsiales with fewer rests per measure indicating higher abundance. For time points at which Rickettsiales was the most abundant microbial taxa, the corresponding measure is highlighted with a cymbal crash. More information at http://www.anl.gov/articles/songs-key... Image: Diatoms under a microscope: These tiny phytoplankton are encased within a silicate cell wall. Credit: Prof. Gordon T. Taylor, Stony Brook University

59 BASIC BIOLOGICAL SCIENCES↗

Mechanisms of Organic Matter Export in Estuaries with Contrasting Carbon Sources

Modifications in land-use and climate will result in shifts in the magnitude and quality of organic matter (OM) transported from wetlands to coastal waters, but differentiation between riverine and wetland OM sources in coastal areas remains a challenge. Here, we evaluate particulate and dissolved OM export dynamics in two representative estuary types—Apalachicola Bay (AP) and Barataria Bay (BB), characterized by primarily blackwater river inputs and high particle abundance, respectively. The magnitude and composition of OM exported from each estuary was evaluated based on seasonal measurements of surface water dissolved organic carbon (DOC), particulate organic carbon (POC), particulate nitrogen (PN), the stable isotopic composition of DOC and POC (d13C-DOC and d13C-POC, respectively), dissolved and particulate lignin phenols, and carbon-normalized dissolved lignin-phenol yields. Discriminant analyses indicated that BB is a more particle-rich and wetland-carbon dominated system compared to AP, which is due to a lack of direct river inputs and high prevalence of wetlands carbon sources (living vs. eroded/submerged). The primary parameters explaining the predominance of particulate and wetland carbon sources in BB were total suspended sediment concentrations, d13C-DOC, d13C-POC, the ratio of cinnamyl to vanillyl phenols in DOC, and the ratio of syringyl to vanillyl phenols for both POC and DOC. In contrast, AP was separated by DOC, particulate C:N ratios, and the ratio of acid to aldehyde lignin phenols for both POC and DOC, indicating a more terrestrial source of organic matter and reflecting the importance of fluvial DOM inputs in this system. Total lignin export (sum of dissolved and particulate) was higher in BB (5.73×105 kg yr-1) than in AP (4.21×105 kg yr-1). Particulate lignin export from BB was greater than the export of dissolved lignin at either BB or AP, suggesting coastal marsh erosion may be driving this comparatively large export of particulate lignin.

aquatic↗

Virioplankton Assemblage Structure in the Lower River and Ocean Continuum of the Amazon

ABSTRACT The Amazon River watershed and its associated plume comprise a vast continental and oceanic area. The microbial activities along this continuum contribute substantially to global carbon and nutrient cycling, and yet there is a dearth of information on the diversity, abundance, and possible roles of viruses in this globally important river. The aim of this study was to elucidate the diversity and structure of virus assemblages of the Amazon River-ocean continuum. Environmental viral DNA sequences were obtained for 12 locations along the river’s lower reach ( n = 5) and plume ( n = 7). Sequence assembly yielded 29,358 scaffolds, encoding 82,546 viral proteins, with 15 new complete viral genomes. Despite the spatial connectivity mediated by the river, virome analyses and physical-chemical water parameters clearly distinguished river and plume ecosystems. Bacteriophages were ubiquitous in the continuum and were more abundant in the transition region. Eukaryotic viruses occurred mostly in the river, while the plume had more viruses of autotrophic organisms ( Prochlorococcus , Synechococcus ) and heterotrophic bacteria ( Pelagibacter ). The viral families Microviridae and Myoviridae were the most abundant and occurred throughout the continuum. The major functions of the genes in the continuum involved viral structures and life cycles, and viruses from plume locations and Tapajós River showed the highest levels of functional diversity. The distribution patterns of the viral assemblages were defined not only by the occurrence of possible hosts but also by water physical and chemical parameters, especially salinity. The findings presented here help to improve understanding of the possible roles of viruses in the organic matter cycle along the river-ocean continuum. IMPORTANCE The Amazon River forms a vast plume in the Atlantic Ocean that can extend for more than 1,000 km. Microbial communities promote a globally relevant carbon sink system in the plume. Despite the importance of viruses for the global carbon cycle, the diversity and the possible roles of viruses in the Amazon are poorly understood. The present work assesses, for the first time, the abundance and diversity of viruses simultaneously in the river and ocean in order to elucidate their possible roles. DNA sequence assembly yielded 29,358 scaffolds, encoding 82,546 viral proteins, with 15 new complete viral genomes from the 12 river and ocean locations. Viral diversity was clearly distinguished by river and ocean. Bacteriophages were the most abundant and occurred throughout the continuum. Viruses that infect eukaryotes were more abundant in the river, whereas phages appeared to have strong control over the host prokaryotic populations in the plume.

Amazon↗

Metapangenomics reveals depth-dependent shifts in metabolic potential for the ubiquitous marine bacterial SAR324 lineage

Abstract Background Oceanic microbiomes play a pivotal role in the global carbon cycle and are central to the transformation and recycling of carbon and energy in the ocean’s interior. SAR324 is a ubiquitous but poorly understood uncultivated clade of Deltaproteobacteria that inhabits the entire water column, from ocean surface waters to its deep interior. Although some progress has been made in elucidating potential metabolic traits of SAR324 in the dark ocean, very little is known about the ecology and the metabolic capabilities of this group in the euphotic and twilight zones. To investigate the comparative genomics, ecology, and physiological potential of the SAR324 clade, we examined the distribution and variability of key genomic features and metabolic pathways in this group from surface waters to the abyss in the North Pacific Subtropical Gyre, one of the largest biomes on Earth. Results We leveraged a pangenomic ecological approach, combining spatio-temporally resolved single-amplified genome, metagenomic, and metatranscriptomic datasets. The data revealed substantial genomic diversity throughout the SAR324 clade, with distinct depth and temporal distributions that clearly differentiated ecotypes. Phylogenomic subclade delineation, environmental distributions, genomic feature similarities, and metabolic capacities revealed strong congruence. The four SAR324 ecotypes delineated in this study revealed striking divergence from one another with respect to their habitat-specific metabolic potentials. The ecotypes living in the dark or twilight oceans shared genomic features and metabolic capabilities consistent with a sulfur-based chemolithoautotrophic lifestyle. In contrast, those inhabiting the sunlit ocean displayed higher plasticity energy-related metabolic pathways, supporting a presumptive photoheterotrophic lifestyle. In epipelagic SAR324 ecotypes, we observed the presence of two types of proton-pumping rhodopsins, as well as genomic, transcriptomic, and ecological evidence for active photoheterotrophy, based on xanthorhodopsin-like light-harvesting proteins. Conclusions Combining pangenomic and both metagenomic and metatranscriptomic profiling revealed a striking divergence in the vertical distribution, genomic composition, metabolic potential, and predicted lifestyle strategies of geographically co-located members of the SAR324 bacterial clade. The results highlight the utility of metapangenomic approaches employed across environmental gradients, to decipher the properties and variation in function and ecological traits of specific phylogenetic clades within complex microbiomes.

59 BASIC BIOLOGICAL SCIENCES↗

Linking deep convection and phytoplankton blooms in the northern Labrador Sea in a changing climate

Wintertime convective mixing plays a pivotal role in the sub-polar North Atlantic spring phytoplankton blooms by favoring phytoplankton survival in the competition between light-dependent production and losses due to grazing and gravitational settling. We use satellite and ocean reanalyses to show that the area-averaged maximum winter mixed layer depth is positively correlated with April chlorophyll concentration in the northern Labrador Sea. A simple theoretical framework is developed to understand the relative roles of winter/spring convection and gravitational sedimentation in spring blooms in this region. Combining climate model simulations that project a weakening of wintertime Labrador Sea convection from Arctic sea ice melt with our framework suggests a potentially significant reduction in the initial fall phytoplankton population that survive the winter to seed the region's spring bloom by the end of the 21st century.

54 ENVIRONMENTAL SCIENCES↗

Modeling the Physical and Biochemical Influence of Ocean Thermal Energy Conversion Plant Discharges into their Adjacent Waters

This paper describes the modeling work by Makai Ocean Engineering, Inc. to simulate the biochemical effects of of the nutrient-enhanced seawater plumes that are discharged by one or several 100 megawatt OTEC plants. The modeling is needed to properly design OTEC plants that can operate sustainably with acceptably low biological impact.

16 TIDAL AND WAVE POWER↗

Complete genome sequence of Polynucleobacter necessarius subsp. asymbioticus type strain (QLW-P1DMWA-1T)

Polynucleobacter necessarius subsp. asymbioticus Hahn et al. 2009 is one of currently two subspecies of P. necessarius. While P. necessarius subsp. asymbioticus is a free-living bacterium, the closely related second subspecies, P. necessarius subsp. necessarius is an obligate endosymbiont living in the cytoplasm of freshwater ciliates of the genus Euplotes aediculatus. The two P. necessarius subspecies were the closest thus far reported phylogenetic neighbors that differ in their lifestyle as obligately free-living vs. obligate endosymbiontic, and they are the only members of the genus Polynucleobacter with completely sequenced genomes. The genome-sequenced strain represents a group of closely related strains not distinguishable by 16S rRNA, 16S-23S ITS or glnA sequences, which is persistent in the home habitat of the strain and frequently contributes > 10% of total bacterial numbers in water samples of the habitat. The 2,159,490 bp long chromosome with a total of 2,088 protein-coding and 48 RNA genes was sequenced as part of the DOE Joint Genome Institute Community Sequencing Program 2006.

59 BASIC BIOLOGICAL SCIENCES↗

Marine Biology, volume II

Symposium on marine biology - growth rate, cycles, transportation, photosynthesis, distribution, sampling, nutrition, and light control of natural phytoplankton ecological systems

CONFERENCE↗

Composition

Water temperature, pH difference, geographical location, and other factors influence on composition and distribution of natural ecological systems of phytoplankton species

PH FACTOR↗