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At least 415 records · Page 23

Investigation of microorganisms in cannabis after heating in a commercial vaporizer

There are concerns about microorganisms present on cannabis materials used in clinical settings by individuals whose health status is already compromised and are likely more susceptible to opportunistic infections from microbial populations present on the materials. Most concerning is administration by inhalation where cannabis plant material is heated in a vaporizer, aerosolized, and inhaled to receive the bioactive ingredients. Heating to high temperatures is known to kill microorganisms including bacteria and fungi; however, microbial death is dependent upon exposure time and temperature. It is unknown whether the heating of cannabis at temperatures and times designated by a commercial vaporizer utilized in clinical settings will significantly decrease the microbial loads in cannabis plant material. To assess this question, bulk cannabis plant material supplied by National Institute on Drug Abuse (NIDA) was used to assess the impact of heating by a commercial vaporizer. Initial method development studies using a cannabis placebo spiked with Escherichia coli were performed to optimize culture and recovery parameters. Subsequent studies were carried out using the cannabis placebo, low delta-9 tetrahydrocannabinol (THC) potency and high THC potency cannabis materials exposed to either no heat or heating for 30 or 70 seconds at 190°C. Phosphate-buffered saline was added to the samples and the samples agitated to suspend the microorganism. Microbial growth after no heat or heating was evaluated by plating on growth media and determining the total aerobic microbial counts and total yeast and mold counts. Overall, while there were trends of reductions in microbial counts with heating, these reductions were not statistically significant, indicating that heating using standard vaporization parameters of 70 seconds at 190°C may not eliminate the existing microbial bioburden, including any opportunistic pathogens. When cultured organisms were identified by DNA sequence analyses, several fungal and bacterial taxa were detected in the different products that have been associated with opportunistic infections or allergic reactions including Enterobacteriaceae, Staphylococcus, Pseudomonas, and Aspergillus.

59 BASIC BIOLOGICAL SCIENCES↗

Expanding the Biological Role of Lipo-Chitooligosaccharides and Chitooligosaccharides in Laccaria bicolor Growth and Development

The role of lipo-chitooligosaccharides (LCOs) as signaling molecules that mediate the establishment of symbiotic relationships between fungi and plants is being redefined. New evidence suggests that the production of these molecular signals may be more of a common trait in fungi than what was previously thought. LCOs affect different aspects of growth and development in fungi. For the ectomycorrhizal forming fungi, Laccaria bicolor, the production and effects of LCOs have always been studied with a symbiotic plant partner; however, there is still no scientific evidence describing the effects that these molecules have on this organism. Here, we explored the physiological, molecular, and metabolomic changes in L. bicolor when grown in the presence of exogenous sulfated and non-sulfated LCOs, as well as the chitooligomers, chitotetraose (CO 4 ), and chitooctaose (CO8). Physiological data from 21 days post-induction showed reduced fungal growth in response to CO and LCO treatments compared to solvent controls. The underlying molecular changes were interrogated by proteomics, which revealed substantial alterations to biological processes related to growth and development. Moreover, metabolite data showed that LCOs and COs caused a downregulation of organic acids, sugars, and fatty acids. At the same time, exposure to LCOs resulted in the overproduction of lactic acid in L. bicolor. Altogether, these results suggest that these signals might be fungistatic compounds and contribute to current research efforts investigating the emerging impacts of these molecules on fungal growth and development.

36 MATERIALS SCIENCE↗

Fast Evaluation of Aircraft Icing Severity Using Machine Learning Based on XGBoost

Aircraft icing represents a serious hazard in aviation which has caused a number of fatal accidents over the years. In addition, it can lead to substantial increase in drag and weight, thus reducing the aerodynamics performance of the airplane. The process of ice accretion on a solid surface is a complex interaction of aerodynamic and environmental variables. The complex relationship makes machine learning-based methods an attractive alternative to traditional numerical simulation-based approaches. In this study, we introduce a purely data-driven approach to find the complex pattern between different flight conditions and aircraft icing severity prediction. The supervised learning algorithm Extreme Gradient Boosting (XGBoost) is applied to establish the prediction framework which makes prediction based on any set of observations. The input flight conditions for the proposed prediction framework are liquid water content, droplet diameter and exposure time. The proposed approach is demonstrated in three cases: maximum ice thickness prediction, icing area prediction and icing severity level evaluation. Performance comparison studies and error analysis are also conducted to verify the effectiveness and performance of the proposed method. Results show that the proposed method has reasonable capability in evaluating aircraft icing severity.

Li, Sibo (ORCID:000000021705844X)↗

Characterization of Oxidative Modifications to Short Peptides Using Low Dose Rate X-Rays

The method of X-ray footprinting and mass spectrometry (XFMS) using high flux synchrotron X-ray sources has become an established method in structural biology and is based on the radiolytic production of hydroxyl radicals, which oxidatively modify protein sidechains. While other methods of producing hydroxyl radicals are available, one benefit of using high flux density sources is that hydroxyl radical scavenging reactions can be minimized, and exposure times kept short to minimize secondary reactions. Here we present an application of the XFMS method using low dose rate X-rays from a commercial instrument. We demonstrate the feasibility of the approach using short peptides, characterizing the oxidative modifications +14, +16, and +32 Da under both aerobic and low oxygen conditions, and we additionally quantify the hydrogen peroxide production for various doses using the low dose rate source. These results provide fundamental information on the oxidative damage to peptides due to hydroxyl radicals using a low dose rate X-ray source.

X-ray methods↗

Azimuthal Anchoring Strength in Photopatterned Alignment of a Nematic

Spatially-varying director fields have become an important part of research and development in liquid crystals. Characterization of the anchoring strength associated with a spatially-varying director is difficult, since the methods developed for a uniform alignment are seldom applicable. Here we characterize the strength of azimuthal surface anchoring produced by the photoalignment technique based on plasmonic metamsaks. The measurements used photopatterned arrays of topological point defects of strength +1 and −1 in thin layers of a nematic liquid crystal. The integer-strength defects split into pairs of half-integer defects with lower elastic energy. The separation distance between the split pair is limited by the azimuthal surface anchoring, which allows one to determine the strength of the latter. The strength of the azimuthal anchoring is proportional to the UV exposure time during the photoalignment of the azobenzene layer.

36 MATERIALS SCIENCE↗

Dynamically Reconfigurable Data Readout of Pixel Detectors for Automatic Synchronization with Data Acquisition Systems

Reconfigurable detectors with dynamically selectable sensing and readout modes are highly desirable for implementing edge computing as well as enabling advanced imaging techniques such as foveation. The concept of a camera system capable of simultaneous passive imaging and dynamic ranging in different regions of the detector is presented. Such an adaptive-autonomous detector with both spatial and temporal control requires programmable window of exposure (time frames), ability to switch between readout modes such as full-frame imaging and zero-suppressed data, modification of the number of pixel data bits and independent programmability for distinct detector regions. In this work, a method is presented for seamlessly changing time frames and readout modes without data corruption while still ensuring that the data acquisition system (DAQ) does not need to stop and resynchronize at each change of setting, thus avoiding significant dead time. Data throughput is maximized by using a minimum unique data format, rather than lengthy frame headers, to differentiate between consecutive frames. A data control and transmitter (DCT) synchronizes data transfer from the pixel to the periphery, reconfigures the data to transmit it serially off-chip, while providing optimized decision support based on a DAQ definable mode. Measurements on a test structure demonstrate that the DCT can operate at 1 GHz in a 65 nm LP CMOS process.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Infrared Observations of 2I/Borisov near Perihelion

As the first interstellar comet, 2I/Borisov provides a unique opportunity to study the surface composition of a comet from another stellar system, particularly whether it has water ice. In order to investigate the nature of 2I/Borisov, we conducted infrared observations close to perihelion. The water ice, if present, is expected to be revealed by absorption features at 1.5 and 2 micron. We therefore used FLAMINGOS-2 mounted on the Gemini south telescope, to carry out deep imaging on 2019 November 30 UT and spectroscopy on 2019 December 7 UT. At first glance, our imaging did not reveal an apparent coma or a cometary tail. This is due to the bright sky background and our short exposure times. Nevertheless we were able to put an upper limit of the size of the nucleus, as well as provide high-precision astrometry that can be used to investigate nongravitational acceleration in the future. Our infrared spectra showed a negative slope, contrary to the results by Yang et al. It is not unheard of for a comet to show a negative slope, and to progressively exhibit a spectrum with decreasing slope. Possible causes of the decreasing slope are an increase in water ice and/or decrease in dust size. Given the fact that our observations were carried out close to perihelion, it is likely that both factors contribute to the decreasing, negative slope of the infrared spectrum.

79 ASTRONOMY AND ASTROPHYSICS↗

The End of Galaxy Surveys

For nearly a century, imaging and spectroscopic surveys of galaxies have given us information about the contents of the universe. We attempt to define the logical end point of such surveys by defining not the next galaxy survey but rather the final galaxy survey at near-infrared wavelengths; this would be the galaxy survey that exhausts the information content useful for addressing extant questions. Such a survey would require incredible advances in a number of technologies, and the survey details will depend on the as yet poorly constrained properties of the earliest galaxies. Using an exposure time calculator, we define nominal surveys for extracting the useful information for three science cases: dark energy cosmology, galaxy evolution, and supernovae (SN). We define scaling relations that trade off sky background, telescope aperture, and focal plane size to allow for a survey of a given depth over a given area. For optimistic assumptions, a 280 m telescope with a marginally resolved focal plane of 20 deg 2 operating at L2 could potentially exhaust the cosmological information content of galaxies in a 10 yr survey. For galaxy evolution (making use of gravitational lensing to magnify the earliest galaxies) and SN, the same telescope would suffice. We discuss the technological advances needed to complete the last galaxy survey. While the final galaxy survey remains well outside of our technical reach today, we present scaling relations that show how we can progress toward the goal of exhausting the information content encoded in the shapes, positions, and colors of galaxies.

79 ASTRONOMY AND ASTROPHYSICS↗

Target Selection and Validation of DESI Luminous Red Galaxies

The Dark Energy Spectroscopic Instrument (DESI) is carrying out a five-year survey that aims to measure the redshifts of tens of millions of galaxies and quasars, including 8 million luminous red galaxies (LRGs) in the redshift range 0.4 < z ≲ 1.0. Here we present the selection of the DESI LRG sample and assess its spectroscopic performance using data from Survey Validation (SV) and the first two months of the Main Survey. The DESI LRG sample, selected using g, r, z, and W1 photometry from the DESI Legacy Imaging Surveys, is highly robust against imaging systematics. The sample has a target density of 605 deg -2 and a comoving number density of 5 × 10 -4 h 3 Mpc -3 in 0.4 < z < 0.8; this is a significantly higher density than previous LRG surveys (such as SDSS, BOSS, and eBOSS) while also extending to z ~ 1. After applying a bright star veto mask developed for the sample, 98.9% of the observed LRG targets yield confident redshifts (with a catastrophic failure rate of 0.2% in the confident redshifts), and only 0.5% of the LRG targets are stellar contamination. The LRG redshift efficiency varies with source brightness and effective exposure time, and we present a simple model that accurately characterizes this dependence. In the appendices, we describe the extended LRG samples observed during SV.

79 ASTRONOMY AND ASTROPHYSICS↗

The DESI Bright Galaxy Survey: Final Target Selection, Design, and Validation

Over the next 5 yr, the Dark Energy Spectroscopic Instrument (DESI) will use 10 spectrographs with 5000 fibers on the 4 m Mayall Telescope at Kitt Peak National Observatory to conduct the first Stage IV dark energy galaxy survey. At z < 0.6, the DESI Bright Galaxy Survey (BGS) will produce the most detailed map of the universe during the dark-energy-dominated epoch with redshifts of >10 million galaxies spanning 14,000 deg 2 . In this work, we present and validate the final BGS target selection and survey design. From the Legacy Surveys, BGS will target an r < 19.5 mag limited sample (BGS Bright), a fainter 19.5 < r < 20.175 color-selected sample (BGS Faint), and a smaller low-z quasar sample. BGS will observe these targets using exposure times scaled to achieve homogeneous completeness and cover the footprint three times. We use observations from the Survey Validation programs conducted prior to the main survey along with simulations to show that BGS can complete its strategy and make optimal use of "bright" time. BGS targets have stellar contamination <1%, and their densities do not depend strongly on imaging properties. BGS Bright will achieve >80% fiber assignment efficiency. Finally, BGS Bright and BGS Faint will achieve >95% redshift success over any observing condition. BGS meets the requirements for an extensive range of scientific applications. BGS will yield the most precise baryon acoustic oscillation and redshift-space distortion measurements at z < 0.4. It presents opportunities for new methods that require highly complete and dense samples (e.g., N-point statistics, multitracers). BGS further provides a powerful tool to study galaxy populations and the relations between galaxies and dark matter.

79 ASTRONOMY AND ASTROPHYSICS↗

Neutrinos from the Galactic Center Hosting a Hypernova Remnant

Similar to star-forming galaxies or starburst galaxies, star-forming regions in our Galaxy can host cosmic-ray (CR) accelerators and rich gas as targets of hadronuclear interaction. By our estimations, the IceCube neutrino observatory might detect muon neutrinos from a CR accelerator associated with a molecular cloud complex in our Galaxy. The associated high-energy gamma-ray emission might be observed by the Cherenkov Telescope Array (CTA), High-Altitude Water Cherenkov Gamma-Ray Observatory (HAWC), and Large High Altitude Air Shower Observatory (LHAASO). Furthermore, taking the Galactic Center (GC) region as an example, we assume that a hypernova exploded in the past in the GC. We simulate the acceleration of CRs in the hypernova remnant (HNR) as well as their confinement and escape. The high-energy protons escape from the HNR, diffuse around the GC, interact with molecular clouds, and then produce gamma-rays and neutrinos. In the optimal cases, the GC would be a promising 100 TeV gamma-ray source for LHAASO’s one-month observation. We propose that neutrino-induced searching for starting track-like and high-energy starting events (HESEs) observed by IceCube, from the GC region with a radius of 1°.8, would help us discover the particle accelerator in the GC or constrain our models. Under the constraint from high-energy gamma-ray observations by the H.E.S.S. telescope, we estimate the exposure time needed to make a significant discovery for the optimal cases. The analysis combining observations of IceCube and ANTARES, starting track-like events and HESEs, future observations by neutrino detectors IceCube-Gen2 and KM3net, and gamma-ray telescopes CTA, HAWC, and LHAASO would help to constrain our models.

79 ASTRONOMY AND ASTROPHYSICS↗

A DESGW Search for the Electromagnetic Counterpart to the LIGO/Virgo Gravitational-wave Binary Neutron Star Merger Candidate S190510g

In this work, we present the results from a search for the electromagnetic counterpart of the LIGO/Virgo event S190510g using the Dark Energy Camera (DECam). S190510g is a binary neutron star (BNS) merger candidate of moderate significance detected at a distance of 227 ± 92 Mpc and localized within an area of 31 (1166) square degrees at 50% (90%) confidence. While this event was later classified as likely nonastrophysical in nature within 30 hours of the event, our short latency search and discovery pipeline identified 11 counterpart candidates, all of which appear consistent with supernovae following offline analysis and spectroscopy by other instruments. Later reprocessing of the images enabled the recovery of six more candidates. Additionally, we implement our candidate selection procedure on simulated kilonovae and supernovae under DECam observing conditions (e.g., seeing and exposure time) with the intent of quantifying our search efficiency and making informed decisions on observing strategy for future similar events. This is the first BNS counterpart search to employ a comprehensive simulation-based efficiency study. We find that using the current follow-up strategy, there would need to be 19 events similar to S190510g for us to have a 99% chance of detecting an optical counterpart, assuming a GW170817-like kilonova. We further conclude that optimization of observing plans, which should include preference for deeper images over multiple color information, could result in up to a factor of 1.5 reduction in the total number of follow-ups needed for discovery.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

DESI Observations of the Andromeda Galaxy: Revealing the Immigration History of Our Nearest Neighbor

Abstract We present Dark Energy Spectroscopic Instrument (DESI) observations of the inner halo of M31, which reveal the kinematics of a recent merger—a galactic immigration event—in exquisite detail. Of the 11,416 sources studied in 3.75 hr of on-sky exposure time, 7438 are M31 sources with well-measured radial velocities. The observations reveal intricate coherent kinematic structure in the positions and velocities of individual stars: streams, wedges, and chevrons. While hints of coherent structures have been previously detected in M31, this is the first time they have been seen with such detail and clarity in a galaxy beyond the Milky Way. We find clear kinematic evidence for shell structures in the Giant Stellar Stream, the Northeast Shelf, and Western Shelf regions. The kinematics are remarkably similar to the predictions of dynamical models constructed to explain the spatial morphology of the inner halo. The results are consistent with the interpretation that much of the substructure in the inner halo of M31 is produced by a single galactic immigration event 1–2 Gyr ago. Significant numbers of metal-rich stars ([Fe/H] > − 0.5) are present in all of the detected substructures, suggesting that the immigrating galaxy had an extended star formation history. We also investigate the ability of the shells and Giant Stellar Stream to constrain the gravitational potential of M31, and estimate the mass within a projected radius of 125 kpc to be log 10 M NFW ( < 125 kpc ) / M ⊙ = 11.80 − 0.10 + 0.12 . The results herald a new era in our ability to study stars on a galactic scale and the immigration histories of galaxies.

79 ASTRONOMY AND ASTROPHYSICS↗

Target Selection and Validation of DESI Quasars

The Dark Energy Spectroscopic Instrument (DESI) survey will measure large-scale structures using quasars as direct tracers of dark matter in the redshift range 0.9 < z < 2.1 and using Lyα forests in quasar spectra at z > 2.1. We present several methods to select candidate quasars for DESI, using input photometric imaging in three optical bands (g, r, z) from the DESI Legacy Imaging Surveys and two infrared bands (W1, W2) from the Wide-field Infrared Survey Explorer. These methods were extensively tested during the Survey Validation of DESI. In this paper, we report on the results obtained with the different methods and present the selection we optimized for the DESI main survey. The final quasar target selection is based on a random forest algorithm and selects quasars in the magnitude range of 16.5 < r < 23. Visual selection of ultra-deep observations indicates that the main selection consists of 71% quasars, 16% galaxies, 6% stars, and 7% inconclusive spectra. Using the spectra based on this selection, we build an automated quasar catalog that achieves a fraction of true QSOs higher than 99% for a nominal effective exposure time of ~1000 s. With a 310 deg -2 target density, the main selection allows DESI to select more than 200 deg -2 quasars (including 60 deg -2 quasars with z > 2.1), exceeding the project requirements by 20%. The redshift distribution of the selected quasars is in excellent agreement with quasar luminosity function predictions.

79 ASTRONOMY AND ASTROPHYSICS↗

Prompt Detection of Fast Optical Bursts with the Vera C. Rubin Observatory

Abstract The transient optical sky has remained largely unexplored on very short timescales. While there have been some experiments searching for optical transients from minutes to years, none have had the capability to distinguish millisecond fast optical bursts (FOBs). Such very fast transients could be the optical counterparts of fast radio bursts, the prompt emission from γ -ray bursts, or other previously unknown phenomena. Here, we investigate a novel approach to the serendipitous detection of FOBs, which relies on searching for anomalous spatial images. In particular, due to their short duration, the seeing-distorted images of FOBs should look characteristically different than those of steady sources in a standard optical exposure of finite duration. We apply this idea to simulated observations with the Vera C. Rubin Observatory, produced by tracing individual photons through a turbulent atmosphere, and down through the optics and camera of the Rubin telescope. We compare these simulated images to steady-source star simulations in 15 s integrations, the nominal Rubin exposure time. We report the classification accuracy results of a neural network classifier for distinguishing FOBs from steady sources. From this classifier, we derive constraints in duration–intensity parameter space for unambiguously identifying FOBs in Rubin observations. We conclude with estimates of the total number of detections of FOB counterparts to FRBs expected during the 10 yr Rubin Legacy Survey of Space and Time.

79 ASTRONOMY AND ASTROPHYSICS↗

Current and Future γ-Ray Searches for Dark Matter Annihilation Beyond the Unitarity Limit

For decades, searches for electroweak-scale dark matter (DM) have been performed without a definitive detection. This lack of success may hint that DM searches have focused on the wrong mass range. A proposed candidate beyond the canonical parameter space is ultraheavy DM (UHDM). In this work, we consider indirect UHDM annihilation searches for masses between 30 TeV and 30 PeV—extending well beyond the unitarity limit at ~100 TeV—and discuss the basic requirements for DM models in this regime. We explore the feasibility of detecting the annihilation signature, and the expected reach for UHDM with current and future very-high-energy (VHE; >100 GeV) γ-ray observatories. Specifically, we focus on three reference instruments: two Imaging Atmospheric Cherenkov Telescope arrays, modeled on VERITAS and CTA-North, and one extended air shower array, motivated by HAWC. With reasonable assumptions on the instrument response functions and background rate, we find a set of UHDM parameters (mass and cross section) for which a γ-ray signature can be detected by the aforementioned observatories. Here, we further compute the expected upper limits for each experiment. With realistic exposure times, the three instruments can probe DM across a wide mass range. At the lower end, it can still have a point-like cross section, while at higher masses the DM could have a geometric cross section, indicative of compositeness.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

DESI Survey Validation Spectra Reveal an Increasing Fraction of Recently Quenched Galaxies at z ∼ 1

Abstract We utilize ∼17,000 bright luminous red galaxies (LRGs) from the novel Dark Energy Spectroscopic Instrument Survey Validation spectroscopic sample, leveraging its deep (∼2.5 hr galaxy −1 exposure time) spectra to characterize the contribution of recently quenched galaxies to the massive galaxy population at 0.4 < z < 1.3. We use Prospector to infer nonparametric star formation histories and identify a significant population of recently quenched galaxies that have joined the quiescent population within the past ∼1 Gyr. The highest-redshift subset (277 at z > 1) of our sample of recently quenched galaxies represents the largest spectroscopic sample of post-starburst galaxies at that epoch. At 0.4 < z < 0.8, we measure the number density of quiescent LRGs, finding that recently quenched galaxies constitute a growing fraction of the massive galaxy population with increasing look-back time. Finally, we quantify the importance of this population among massive ( log ( M ⋆ / M ⊙ ) > 11.2) LRGs by measuring the fraction of stellar mass each galaxy formed in the gigayear before observation, f 1 Gyr . Although galaxies with f 1 Gyr > 0.1 are rare at z ∼ 0.4 (≲0.5% of the population), by z ∼ 0.8, they constitute ∼3% of massive galaxies. Relaxing this threshold, we find that galaxies with f 1 Gyr > 5% constitute ∼10% of the massive galaxy population at z ∼ 0.8. We also identify a small but significant sample of galaxies at z = 1.1–1.3 that formed with f 1 Gyr > 50%, implying that they may be analogs to high-redshift quiescent galaxies that formed on similar timescales. Future analysis of this unprecedented sample promises to illuminate the physical mechanisms that drive the quenching of massive galaxies after cosmic noon.

79 ASTRONOMY AND ASTROPHYSICS↗