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At least 415 records · Page 23

Determining the size and concentration dependence of gold nanoparticles in vitro cytotoxicity (IC{sub 50}) test using WST-1 assay

Gold nanoparticles (AuNPs) received a great deal of attention for biomedical applications, especially in diagnostic imaging and therapeutics. Even though AuNPs have potential benefits in biomedical applications, the impact of AuNPs on human and environmental health still remains unclear. The use of AuNPs which is a high-atomic-number materials, provide advantages in terms of radiation dose enhancement. However, before this can become a clinical reality, cytotoxicity of the AuNPs has to be carefully evaluated. Cytotoxicity test is a rapid, standardized test that is very sensitive to determine whether the nanoparticles produced are harmful or benign on cellular components. In this work the size and concentration dependence of AuNPs cytotoxicity in breast cancer cell lines (MCF-7) are tested by using WST-1 assay. The sizes of AuNPs tested were 13 nm, 50 nm, and 70 nm. The cells were seeded in the 96-well plate and were treated with different concentrations of AuNPs by serial dilution for each size of AuNPs. The high concentration of AuNPs exhibit lower cell viability compared to low concentration of AuNPs. We quantified the toxicity of AuNPs in MCF-7 cell lines by determining the IC{sub 50} values in WST-1 assays. The IC{sub 50} values (inhibitory concentrations that effected 50% growth inhibition) of 50 nm AuNPs is lower than 13 nm and 70 nm AuNPs. Mean that, 50nm AuNPs are more toxic to the MCF-7 cells compared to smaller and larger sizes AuNPs. The presented results clearly indicate that the cytotoxicity of AuNPs depend not only on the concentration, but also the size of the nanoparticles.

60 APPLIED LIFE SCIENCES↗

Effect of ethanol concentrations on temperature driven structural changes of chymotrypsin inhibitor 2

A series of atomistic molecular dynamics (MD) simulations of a small enzymatic protein Chymotrypsin Inhibitor 2 (CI2) in water-ethanol mixed solutions were carried out to explore the underlying mechanism of ethanol driven conformational changes of the protein. Efforts have been made to probe the influence of ethanol concentrations ranging from 0% to 75% (v/v) at ambient condition (300 K (T1)) and at elevated temperatures (375 K (T2) and 450 K (T3)) to investigate the temperature induced conformational changes of the protein further. Our study showed that the effect of varying ethanol concentrations on protein’s structure is almost insignificant at T1 and T2 temperatures whereas at T3 temperature, partial unfolding of CI2 in 10% ethanol solution followed by full unfolding of the protein at ethanol concentrations above 25% occurs. However, interestingly, at T3 temperature CI2’s native structure was found to be retained in pure water (0% ethanol solution) indicating that the cosolvent ethanol do play an important role in thermal denaturation of CI2. Such observations were quantified in the light of root-mean-square deviations (RMSDs) and radius of gyration. Although higher RMSD values of β-sheet over α-helix indicate complete destruction of the β-structure of CI2 at high ethanol concentrations, the associated time scale showed that the faster melting of α-helix happens over β-sheet. Around 60%-80% of initial native contacts of the protein were found broken with the separation of hydrophobic core consisting eleven residues at ethanol concentrations greater than 25%. This leads protein to expand with the increase in solvent accessible surface area. The interactions between protein and solvent molecules showed that protein’s solvation shell preferred to accommodate ethanol molecules as compared to water thereby excluded water molecules from CI2’s surface. Further, concentration dependent differential self-aggregation behavior of ethanol is likely to regulate the replacement of relatively fast diffused water by low diffused ethanol molecules from protein’s surface during the unfolding process.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

COSMIC EMULATION: THE CONCENTRATION-MASS RELATION FOR wCDM UNIVERSES

The concentration-mass relation for dark matter-dominated halos is one of the essential results expected from a theory of structure formation. We present a simple prediction scheme, a cosmic emulator, for the concentration-mass (c-M) relation as a function of cosmological parameters for wCDM models. The emulator is constructed from 37 individual models, with three nested N-body gravity-only simulations carried out for each model. The mass range covered by the emulator is 2 Multiplication-Sign 10{sup 12} M{sub Sun} < M < 10{sup 15} M{sub Sun} with a corresponding redshift range of z = 0-1. Over this range of mass and redshift, as well as the variation of cosmological parameters studied, the mean halo concentration varies from c {approx} 2 to c {approx} 8. The distribution of the concentration at fixed mass is Gaussian with a standard deviation of one-third of the mean value, almost independent of cosmology, mass, and redshift over the ranges probed by the simulations. We compare results from the emulator with previously derived heuristic analytic fits for the c-M relation, finding that they underestimate the halo concentration at high masses. Using the emulator to investigate the cosmology dependence of the c-M relation over the currently allowable range of values, we find-not surprisingly-that {sigma}{sub 8} and {omega}{sub m} influence it considerably, but also that the dark energy equation-of-state parameter w has a substantial effect. In general, the concentration of lower-mass halos is more sensitive to changes in cosmological parameters as compared to cluster mass halos. The c-M emulator is publicly available from http://www.hep.anl.gov/cosmology/CosmicEmu.

79 ASTRONOMY AND ASTROPHYSICS↗

THE ZURICH ENVIRONMENTAL STUDY (ZENS) OF GALAXIES IN GROUPS ALONG THE COSMIC WEB. II. GALAXY STRUCTURAL MEASUREMENTS AND THE CONCENTRATION OF MORPHOLOGICALLY CLASSIFIED SATELLITES IN DIVERSE ENVIRONMENTS

We present structural measurements for the galaxies in the 0.05 < z < 0.0585 groups of the Zurich Environmental Study, aimed at establishing how galaxy properties depend on four environmental parameters: group halo mass (M{sub GROUP}), group-centric distance (R/R{sub 200}), ranking into central or satellite, and large-scale structure density (δ{sub LSS}). Global galaxy structure is quantified both parametrically and non-parametrically. We correct all these measurements for observational biases due to point-spread function blurring and surface brightness effects as a function of galaxy size, magnitude, steepness of light profile, and ellipticity. Structural parameters are derived also for bulges, disks, and bars. We use the galaxy bulge-to-total ratios (B/T) together with the calibrated non-parametric structural estimators to implement a quantitative morphological classification that maximizes purity in the resulting morphological samples. We investigate how the concentration C of satellite galaxies depends on galaxy mass for each Hubble type and on M{sub GROUP}, R/R{sub 200}, and δ{sub LSS}. At galaxy masses M ≥ 10{sup 10} M{sub ☉}, the concentration of disk satellites increases with increasing stellar mass separately within each morphological bin of B/T. The known increase in concentration with stellar mass for disk satellites is thus due, at least in part, to an increase in galaxy central stellar density at constant B/T. The correlation between concentration and galaxy stellar mass becomes progressively steeper for later morphological types. The concentration of disk satellites shows a barely significant dependence on δ{sub LSS} or R/R{sub 200}. The strongest environmental effect is found with group mass for >10{sup 10} M{sub ☉} disk-dominated satellites, which are ∼10% more concentrated in high mass groups than in lower mass groups.

79 ASTRONOMY AND ASTROPHYSICS↗

Magnetic flux concentration and zonal flows in magnetorotational instability turbulence

Accretion disks are likely threaded by external vertical magnetic flux, which enhances the level of turbulence via the magnetorotational instability (MRI). Using shearing-box simulations, we find that such external magnetic flux also strongly enhances the amplitude of banded radial density variations known as zonal flows. Moreover, we report that vertical magnetic flux is strongly concentrated toward low-density regions of the zonal flow. Mean vertical magnetic field can be more than doubled in low-density regions, and reduced to nearly zero in high-density regions in some cases. In ideal MHD, the scale on which magnetic flux concentrates can reach a few disk scale heights. In the non-ideal MHD regime with strong ambipolar diffusion, magnetic flux is concentrated into thin axisymmetric shells at some enhanced level, whose size is typically less than half a scale height. We show that magnetic flux concentration is closely related to the fact that the turbulent diffusivity of the MRI turbulence is anisotropic. In addition to a conventional Ohmic-like turbulent resistivity, we find that there is a correlation between the vertical velocity and horizontal magnetic field fluctuations that produces a mean electric field that acts to anti-diffuse the vertical magnetic flux. The anisotropic turbulent diffusivity has analogies to the Hall effect, and may have important implications for magnetic flux transport in accretion disks. The physical origin of magnetic flux concentration may be related to the development of channel flows followed by magnetic reconnection, which acts to decrease the mass-to-flux ratio in localized regions. The association of enhanced zonal flows with magnetic flux concentration may lead to global pressure bumps in protoplanetary disks that helps trap dust particles and facilitates planet formation.

79 ASTRONOMY AND ASTROPHYSICS↗

The music of clash: predictions on the concentration-mass relation

We present an analysis of the MUSIC-2 N-body/hydrodynamical simulations aimed at estimating the expected concentration-mass relation for the CLASH (Cluster Lensing and Supernova Survey with Hubble) cluster sample. We study nearly 1,400 halos simulated at high spatial and mass resolution. We study the shape of both their density and surface-density profiles and fit them with a variety of radial functions, including the Navarro-Frenk-White (NFW), the generalized NFW, and the Einasto density profiles. We derive concentrations and masses from these fits. We produce simulated Chandra observations of the halos, and we use them to identify objects resembling the X-ray morphologies and masses of the clusters in the CLASH X-ray-selected sample. We also derive a concentration-mass relation for strong-lensing clusters. We find that the sample of simulated halos that resembles the X-ray morphology of the CLASH clusters is composed mainly of relaxed halos, but it also contains a significant fraction of unrelaxed systems. For such a heterogeneous sample we measure an average two-dimensional concentration that is ∼11% higher than is found for the full sample of simulated halos. After accounting for projection and selection effects, the average NFW concentrations of CLASH clusters are expected to be intermediate between those predicted in three dimensions for relaxed and super-relaxed halos. Matching the simulations to the individual CLASH clusters on the basis of the X-ray morphology, we expect that the NFW concentrations recovered from the lensing analysis of the CLASH clusters are in the range [3-6], with an average value of 3.87 and a standard deviation of 0.61.

79 ASTRONOMY AND ASTROPHYSICS↗

A COMPACT CONCENTRATION OF LARGE GRAINS IN THE HD 142527 PROTOPLANETARY DUST TRAP

A pathway to the formation of planetesimals, and eventually giant planets, may occur in concentrations of dust grains trapped in pressure maxima. Dramatic crescent-shaped dust concentrations have been seen in recent radio images at submillimeter wavelengths. These disk asymmetries could represent the initial phases of planet formation in the dust trap scenario, provided that grain sizes are spatially segregated. A testable prediction of azimuthal dust trapping is that progressively larger grains should be more sharply confined and should follow a distribution that is markedly different from the gas. However, gas tracers such as {sup 12}CO and the infrared emission from small grains are both very optically thick where the submillimeter continuum originates, so previous observations have been unable to test the trapping predictions or to identify compact concentrations of larger grains required for planet formation by core accretion. Here we report multifrequency observations of HD 142527, from 34 to 700 GHz, that reveal a compact concentration of grains approaching centimeter sizes, with a few Earth masses, embedded in a large-scale crescent of smaller, submillimeter-sized particles. The emission peaks at wavelengths shorter than ∼1 mm are optically thick and trace the temperature structure resulting from shadows cast by the inner regions. Given this temperature structure, we infer that the largest dust grains are concentrated in the 34 GHz clump. We conclude that dust trapping is efficient enough for grains observable at centimeter wavelengths to lead to compact concentrations.

79 ASTRONOMY AND ASTROPHYSICS↗

An Advanced Cooling Device for Concentrated Photovoltaic Systems

Concentrated photovoltaics (CPV) have the potential to significantly enhance the energy conversion utilization of solar panels and reduce solar generation costs, making them a crucial area of advancement in solar power generation technology. However, the concentration of sunlight can lead to overheating of solar panels, resulting in a notable reduction in both the efficiency of solar power generation and the lifespan of the panels. This challenge remains the predominant technical hurdle that hinders the application of concentrated photovoltaic power generation technology. In this study, we propose a new cooling method for concentrated photovoltaic power generation systems via an integrated approach of incorporating Phase-Change Thermal Storage (PCTS) and Thermoelectric Generator (TEG) technology. This new method not only enhances the overall system's electricity generation efficiency but also effectively resolves the technical challenge of concentrated photovoltaic panel overheating issues, ensuring the continuity of concentrated photovoltaic power generation and extending the lifespan of solar panels and their components. In order to make full use of the wasted heat generated by photovoltaic power generation and effectively improve the power generation efficiency of the system, this work developed a phase change heat storage device based on a phase change material. This device uses the temperature difference between day and night to recover wasted heat from photovoltaic power generation. Through integration with the thermoelectric power generation system, thermal energy can be converted into electrical energy. In addition, the Peltier effect of thermoelectric materials is used to construct a photovoltaic panel overheating protection system, which significantly improves the reliability and service life of the system.

14 SOLAR ENERGY↗

Ice Concentration Retrieval in Stratiform Mixed-phase Clouds Using Cloud Radar Reflectivity Measurements and 1D Ice Growth Model Simulations

Measurement of ice number concentration in clouds is important but still challenging. Stratiform mixed-phase clouds (SMCs) provide a simple scenario for retrieving ice number concentration from remote sensing measurements. The simple ice generation and growth pattern in SMCs offers opportunities to use cloud radar reflectivity (Ze) measurements and other cloud properties to infer ice number concentration quantitatively. To understand the strong temperature dependency of ice habit and growth rate quantitatively, we develop a 1-D ice growth model to calculate the ice diffusional growth along its falling trajectory in SMCs. The radar reflectivity and fall velocity profiles of ice crystals calculated from the 1-D ice growth model are evaluated with the Atmospheric Radiation Measurements (ARM) Climate Research Facility (ACRF) ground-based high vertical resolution radar measurements. Combining Ze measurements and 1-D ice growth model simulations, we develop a method to retrieve the ice number concentrations in SMCs at given cloud top temperature (CTT) and liquid water path (LWP). The retrieved ice concentrations in SMCs are evaluated with in situ measurements and with a three-dimensional cloud-resolving model simulation with a bin microphysical scheme. These comparisons show that the retrieved ice number concentrations are within an uncertainty of a factor of 2, statistically.

1-D ice growth↗

Heliostats with Adjustable Shape for High Concentration throughout the Day

Our motivation is to develop more efficient heliostats that can provide commercially viable solar thermal power at temperatures > 800°C. Such high temperatures will enable high-temperature industrial processes, as well as electrical generation after sunset with high efficiency. The importance of this research is that such heliostats have the potential to substantially expand the global use of solar energy, by adding solar thermal power as a major component. Thermal solar currently accounts for only 1% of all solar power (with PV being the rest), with heliostat fields providing just 0.25%. Our goals have been 1) to demonstrate a technical improvement for heliostats that can enable fields of them to more efficiently power receivers and reactors, and 2) to show a path to low-cost mass production. Our solution uses new opto-mechanical technology to correct a fundamental deficiency of present heliostats that limits their concentration, namely that they have fixed shape. Most of today’s heliostat research does not address this, but is directed simply toward cost reduction in an effort to make heliostats commercially viable. We are motivated to explore also improving heliostat efficiency, which can be done by continually changing their shape to maximize the concentration of sunlight throughout the day. This is not a new concept, but it has never been implemented in a practical, cost-effective way that approaches the theoretical limit to concentration while also improving mechanical performance; this is our goal. Our major accomplishments have been: 1) We have realized the planned design, construction, and test of a prototype heliostat that achieves the required shape changes in an 8 m2 single-piece glass mirror. The mirror is attached to a steel support frame that is automatically mechanically twisted by the heliostat drives that orient the mirror to direct sunlight to the tower-mounted receiver. Closed-loop tracking is done using a new beamsplitter camera that exploits the target-oriented mount configuration. Field tests of the heliostat show that the light is reflected through the day to always form a disc image of the sun, as needed to obtain the highest concentration. 2) We have developed the design for a field of 431 heliostats to deliver annual average of 1 MW of thermal power at 3,000 sun concentration, matched to a high-temperature ≥ 1000°C chemical reactor. 3) We have also developed, beyond the original stated goals of the project, a new concept for closed-loop tracking and shape-sensing for all the heliostats in the above field, using just 6 cameras around the concentrated reactor focus. Our research adds to the understanding of solar thermal energy by its demonstration of the technical effectiveness of a higher performing heliostat, and by its concept for a new powerful method for real-time tracking and shape sensing in the field, as described above. We have studied the economic feasibility of fields of our twisting heliostats to provide high- temperature heat at a price competitive with that of burning gas, to satisfy the DOE’s studied zero-emissions scenario, where the gas price has to include the cost of carbon capture. The project has the potential to greatly benefit the public if it helps limit global warming by 1) reducing carbon emission from industrial heating, which is currently a major contributor to the 40-billion-ton annual increase in atmospheric CO 2 . 2) Ultimately, the technology could prove to be the least expensive method to power direct air capture of CO 2 on the very large scale needed to remove the 1 trillion-ton excess of CO 2 already in the atmosphere.

14 SOLAR ENERGY↗

Modeling and Calculation of Optical-Geometric Characteristics of a Solar Concentrator with Flat Fresnel Mirrors

The basic characteristics of a concentrator, such as the number of reflectors, their coordinates and orientation, the degree of concentration, and others are determined with the use of the optical-geometric approach depending on the configuration parameters of the concentrator. Based on the presented formulas and numerical results, it is possible to determine the parameters of an arbitrary configuration of a linear-focus concentrator with flat Fresnel reflectors located on a parabolic base. The specific features of the distribution of the energy density over the receiver are determined. The paths of solar beams in the system and formation features of the transverse focal lines of the concentrated optical radiation are graphically analyzed for the case of the exact orientation of the concentrator on the Sun.

79 ASTRONOMY AND ASTROPHYSICS↗

A Study of autunite dissolution in the presence of Shewanella oneidensis MR1 and different bicarbonate concentrations - 15736

Remediation of uranium at Hanford Site 300 area via tripolyphosphate injections could sequester uranium (VI) in an insoluble form, creating different types of uranium phosphate minerals like autunite. The factors that influence the dissolution of such minerals come into question. Bacteria can play a significant role in the dissolution of minerals and the formation of secondary minerals. Formed autunite, as a phosphorus-containing mineral, can attract bacteria to liberate phosphorus, meeting their nutrient requirements and causing uranium release back into the environment. Previous studies have been conducted under aerobic conditions. This research was extended to investigate the stability of the autunite mineral under anaerobic conditions pertaining to the Hanford Site, and to study the effect of facultative anaerobic bacteria on the uranium (VI) release from autunite. Objectives: - Evaluate bacterial interactions with uranium (VI) by focusing on facultative anaerobic bacteria, Shewanella Oneidensis MR1. - Study the effect of bacteria on the dissolution of the uranyl phosphate solid phases created as a result of sodium tripolyphosphate injections into the subsurface at the Hanford 300 Area. - Evaluate the role of anaerobic bacteria as one of the factors affecting the outcome of environmental remediation. Experimental Approach: - 16 crimp-sealed bottles were prepared with 50 ml of media solution and 90 mg (4.4 mM of uranium VI) Ca-autunite mineral obtained from Hanford site. Bottles were inoculated with Shewanella Oneidensis MR1 strains when autunite was equilibrated with the media solutions. Bacteria was obtained from Pacific Northwestern National Laboratory (PNNL). - Solution media preparation: 20 mM NaHepes buffer: pH=7, 10 mM Lactate plus bicarbonate in four concentrations (0, 3, 5 and 10 of KHCO{sub 3}) and a bacteria-free control. Samples were taken from the supernatant solutions of the experimental and control bottles. Wet and dry digestion was performed until a white solid residue was acquired. Dilution was made on solid samples for KPA analysis to determine concentration of U (VI) as function of time. The results suggests that in the absence of HCO{sub 3}{sup -} there was a decrease in the of uranium (VI) concentration due to metal reducing bacteria Shewanella Oneidensis MR1, but for higher concentrations of HCO{sub 3}{sup -}, there is no significant difference in uranium (VI) reduction before and after inoculation with bacteria. - Although bottles were sealed, there is an hypothesis that it was oxygen restricted but not strictly performed under anaerobic conditions. For that matter there is a reason to repeat the experiment using the glove box to understand the uranium (VI) reduction mechanism and behavior of bacteria with oxic/anoxic interfaces. Conclusions: - Higher bicarbonate concentrations might interfere with uranium (VI) reduction by anaerobic bacteria. - It is necessary to reduce time of sampling before the inoculation and prolong sampling time after inoculation. - It might be necessary to increase concentration of lactate. Future Work: - Conduct experiment in strictly anaerobic conditions by using the glove box and evaluate if further reduction of uranium (VI) is obtained in the presence of carbonates (HCO{sub 3}{sup -}). - Finalize analysis of calcium and phosphorous via ICP instrument.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Effect of salt concentration profiles on protrusion growth in lithium-polymer-lithium cells

In this report, the formation of dendrites and other protrusions on lithium metal anodes is a subject of continued interest due to the potential to incorporate these anodes in next-generation rechargeable batteries with increased energy densities. Solid polymer electrolytes show improved stability against lithium metal compared to liquid carbonate electrolytes. We have studied the effect of salt concentration on the formation of protrusions formed on electrodeposited lithium through a rigid block copolymer electrolyte, polystyrene-block-poly(ethylene oxide) (PS-b-PEO or SEO), in a lithium-lithium symmetric cell. The cell lifetime decreases by a factor of 100 when salt concentration is increased by a factor of 5. Our main objective is to understand the reason for this observation. We show that this decrease is not due to a salt-induced change of the morphology of the block-copolymer electrolyte, nor is it due to a salt-induced change of mechanical properties. We use an approach based on Newman's concentrated solution theory to fully characterize ion transport in the block-copolymer electrolyte, and report the conductivity, salt diffusion coefficient, cation transference number, and thermodynamic factor. Neither cell lifetime nor protrusion density in failed cells correlate with any of these electrochemical parameters. However, the electrochemical parameters can be used to predict salt concentration profiles in our symmetric cells. We posit that an important parameter in protrusion growth is the magnitude of the salt concentration gradient, Δ. We observe a direct correlation between Δ and lithium protrusion growth.

25 ENERGY STORAGE↗

Impacts of the East Asian Monsoon on springtime dust concentrations over China

We use 150 year preindustrial simulations of the Community Earth System Model to quantify the impacts of the East Asian Monsoon strength on interannual variations of springtime dust concentrations over China. The simulated interannual variations in March-April-May (MAM) dust column concentrations range between 20–40% and 10–60% over eastern and western China, respectively. The dust concentrations over eastern China correlate negatively with the East Asian Monsoon (EAM) index, which represents the strength of monsoon, with a regionally averaged correlation coefficient of 0.64. Relative to the strongest EAM years, MAMdust concentrations in the weakest EAM years are higher over China, with regional relative differences of 55.6%, 29.6%, and 13.9% in the run with emissions calculated interactively and of 33.8%, 10.3%, and 8.2% over eastern, central, and western China, respectively, in the run with prescribed emissions. Both interactive run and prescribed emission run show the similar pattern of climate change between the weakest and strongest EAM years. Strong anomalous northwesterly and westerly winds over the Gobi and Taklamakan deserts during the weakest EAM years result in larger transport fluxes, and thereby increase the dust concentrations over China. Here, these differences in dust concentrations between the weakest and strongest EAM years (weakest-strongest) lead to the change in the net radiative forcing by up to -8 and -3Wm -2 at the surface, compared to -2.4 and +1.2Wm -2 at the top of the atmosphere over eastern and western China, respectively.

58 GEOSCIENCES↗

Drivers of Seasonal Variability in Marine Boundary Layer Aerosol Number Concentration Investigated Using a Steady State Approach

Marine boundary layer (MBL) aerosol particles affect the climate through their interaction with MBL clouds. Although both MBL clouds and aerosol particles have pronounced seasonal cycles, the factors controlling seasonal variability of MBL aerosol particle concentration are not well-constrained. In this paper an aerosol budget is constructed representing the effects of wet deposition, free-tropospheric entrainment, primary surface sources, and advection on the MBL accumulation mode aerosol number concentration (N a ). These terms are further parameterized, and by assuming that on seasonal timescales N a is in steady state, the budget equation is rearranged to form a diagnostic equation for N a based on observable variables. Using data primarily collected in the subtropical northeast Pacific during the MAGIC campaign (Marine ARM (Atmospheric Radiation Measurement) GPCI (GCSS Pacific Cross-section Intercomparison) Investigation of Clouds), estimates of both mean summer and winter N a concentrations are made using the simplified steady-state model and seasonal mean observed variables, and are found to match well with the observed N a . To attribute the modeled difference between summer and winter aerosol concentrations to individual observed variables (e.g. precipitation rate, free-tropospheric aerosol number concentration), a local sensitivity analysis is combined with the seasonal difference in observed variables. This analysis shows that despite wintertime precipitation frequency being lower than summer, the higher winter precipitation rate accounted for approximately 60% of the modeled seasonal difference in N a , which emphasizes the importance of marine stratocumulus precipitation in determining MBL aerosol concentrations on longer time scales.

54 ENVIRONMENTAL SCIENCES↗

Fabrication and Characterization of Composite Membranes for the Concentration of Lithium Containing Solutions Using Forward Osmosis

In this study, an energy and highly time efficient, cost effective, and scalable forward osmosis (FO) composite polyvinylidene fluoride (PVDF) membrane is developed to concentrate lithium chloride recovered from naturally occurring geothermal brine resources. Dense thin robust defect-free Kynar-PVDF membranes are fabricated on porous PVDF hollow fiber supports. Various membrane fabrication parameters such as membrane thickness, coating solution concentration, cross-linker concentration, and curing temperature are studied to develop a defect-free dense membrane. Advanced characterization tools such as FIB-SEM, Raman, FTIR spectroscopy, and XRD are used to study the surface and cross-sectional morphology, chemistry of crosslinking, crystallinity and chemical structure of the dense PVDF membrane. FO is utilized to concentrate LiCl in the solution from 36 to 175 g L -1 with an average water transfer rate of 0.450 L m -2 h -1 (LMH) at 85 °C. Furthermore, the membrane showed stable performance for more than 550 h while maintaining the purity of the concentrated lithium chloride solution of >99.99 wt%. Overall, the results suggest that the FO-based technology is an innovative, time and energy efficient approach compared to the conventional technologies for concentration of battery grade LiCl for application in lithium ion batteries and other clean energy technologies.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Temporal variations of methane concentration and isotopic composition in groundwater of the St. Lawrence Lowlands, eastern Canada

Dissolved methane concentrations in shallow groundwater are known to vary both spatially and temporally. The extent of these variations is poorly documented although this knowledge is critical for distinguishing natural fluctuations from anthropogenic impacts stemming from oil and gas activities. This issue was addressed as part of a groundwater research project aiming to assess the risk of shale gas development for groundwater quality over a 500-km{sup 2} area in the St. Lawrence Lowlands (Quebec, Canada). A specific study was carried out to define the natural variability of methane concentrations and carbon and hydrogen isotope ratios in groundwater, as dissolved methane is naturally ubiquitous in aquifers of this area. Monitoring was carried out over a period of up to 2.5 years in seven monitoring wells. Results showed that for a given well, using the same sampling depth and technique, methane concentrations can vary over time from 2.5 to 6 times relative to the lowest recorded value. Methane isotopic composition, which is a useful tool to distinguish gas origin, was found to be stable for most wells, but varied significantly over time in the two wells where methane concentrations are the lowest. The use of concentration ratios, as well as isotopic composition of methane and dissolved inorganic carbon (DIC), helped unravel the processes responsible for these variations. This study indicates that both methane concentrations and isotopic composition, as well as DIC isotopes, should be regularly monitored over at least 1 year to establish their potential natural variations prior to hydrocarbon development.

54 ENVIRONMENTAL SCIENCES↗

Nanoscale mapping of point defect concentrations with $\mathrm{4D-STEM}$

Vacancies are missing atoms in a crystalline material, and occur both at equilibrium (varying with temperature) and out of equilibrium such as when crystalline materials are damaged with radiation or corrosion. While we know of their importance, particularly regarding diffusive mechanisms, it is not straightforward to experimentally measure their concentration or to visualize them directly. Traditionally, measurements such as positron annihilation spectroscopy and to some degree X-ray diffraction can measure average concentrations, but generally lack the ability to visualize or quantify a heterogenous concentration of vacancies that can occur at the level of individual defects and microstructural features. Here, we present a method to map vacancy concentrations and their distribution using local lattice parameter measurements with a high-resolution electron microscope. Our method utilizes a Au thin film as a model to demonstrate the method via four-dimensional scanning transmission electron microscopy (4D-STEM) by correlating the differences between changes in lattice parameter and the volumetric thermal expansion during in situ heating experiments. The vacancy mapping methodology is also applied to non-equilibrium defects accumulated in pure Al via knock-on electron beam irradiation. Our method demonstrates the ability to map point defect concentrations in heterogeneous systems in situ with nanometer spatial resolution. The result is a technique that can provide direct measurements of vacancy concentrations at the level of individual defects in studies of materials in and out of equilibrium.

36 MATERIALS SCIENCE↗