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At least 415 records · Page 23

Isolation, Structural Analysis and Biological Activity Assays of Biselisabethoxanes A and B: Two Dissymmetric Bis-Diterpenes from the Southwestern Caribbean Sea Gorgonian Coral Pseudopterogorgia elisabethae

Two novel dissymmetric diterpenoids, biselisabethoxanes A and B (1 and 2), were isolated from the hexane extracts of the gorgonian coral Pseudopterogorgia elisabethae. Biselisabethoxane A (1) represents the first example of a marine-derived C40 dimer made of two distinct diterpene fragments, whereas biselisabethoxane B (2) is a fused heterodimer stemming from coupling of two amphilectane-based fragments. The structures of 1 and 2 were elucidated based on 1D and 2D NMR spectral data analysis. The molecular structure of 1 was subsequently confirmed by X-ray crystallographic analysis. When evaluated for their inhibitory effects in a series of well-established biological activity assays the isolated compounds were shown to moderately inhibit the growth of Mycobacterium tuberculosis.

59 BASIC BIOLOGICAL SCIENCES↗

Moisture and Structural Analysis for High Performance Hybrid Wall Assemblies

This report describes the work conducted by the Building Science Corporation (BSC) Building America Research Team's 'Energy Efficient Housing Research Partnerships' project. Based on past experience in the Building America program, they have found that combinations of materials and approaches—in other words, systems—usually provide optimum performance. No single manufacturer typically provides all of the components for an assembly, nor has the specific understanding of all the individual components necessary for optimum performance.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Space Shuttle Main Engine structural analysis and data reduction/evaluation. Volume 6: Primary nozzle diffuser analysis

The primary nozzle diffuser routes fuel from the main fuel valve on the Space Shuttle Main Engine (SSME) to the nozzle coolant inlet mainfold, main combustion chamber coolant inlet mainfold, chamber coolant valve, and the augmented spark igniters. The diffuser also includes the fuel system purge check valve connection. A static stress analysis was performed on the diffuser because no detailed analysis was done on this part in the past. Structural concerns were in the area of the welds because approximately 10 percent are in areas inaccessible by X-ray testing devices. Flow dynamics and thermodynamics were not included in the analysis load case. Constant internal pressure at maximum SSME power was used instead. A three-dimensional, finite element method was generated using ANSYS version 4.3A on the Lockheed VAX 11/785 computer to perform the stress computations. IDEAS Supertab on a Sun 3/60 computer was used to create the finite element model. Rocketdyne drawing number RS009156 was used for the model interpretation. The flight diffuser is denoted as -101. A description of the model, boundary conditions/load case, material properties, structural analysis/results, and a summary are included for documentation.

Foley, Michael J.↗

Structural analysis of ITER magnet feeders

This paper summarizes the results of the static structural analyses, which were conducted in support of the ITER magnet feeder design with the aim of validating certain components against the structural design criteria. While almost every feeder has unique features, they all share many common constructional elements and the same functional specifications. The analysis approach to assess the load conditions and stresses that have driven the design is equivalent for all feeders, except for particularities that needed to be modeled in each case. The mechanical analysis of the feeders follows the sub-modeling approach: the results of the global mechanical model of a feeder assembly are used as input for the detailed models of the feeder' sub-assemblies or single components. Examples of such approach, including the load conditions, stress assessment criteria and solutions for the most critical components, are discussed. It has been concluded that the feeder system is safe in the referential operation scenarios. (authors)

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Structural Analysis of Papain-Like NlpC/P60 Superfamily Enzymes with a Circularly Permuted Topology Reveals Potential Lipid Binding Sites

NlpC/P60 superfamily papain-like enzymes play important roles in all kingdoms of life. Two members of this superfamily, LRAT-like and YaeF/YiiX-like families, were predicted to contain a catalytic domain that is circularly permuted such that the catalytic cysteine is located near the C-terminus, instead of at the N-terminus. These permuted enzymes are widespread in virus, pathogenic bacteria, and eukaryotes. We determined the crystal structure of a member of the YaeF/YiiX-like family from Bacillus cereus in complex with lysine. The structure, which adopts a ligand-induced, 'closed' conformation, confirms the circular permutation of catalytic residues. A comparative analysis of other related protein structures within the NlpC/P60 superfamily is presented. Permutated NlpC/P60 enzymes contain a similar conserved core and arrangement of catalytic residues, including a Cys/His-containing triad and an additional conserved tyrosine. More surprisingly, permuted enzymes have a hydrophobic S1 binding pocket that is distinct from previously characterized enzymes in the family, indicative of novel substrate specificity. Further analysis of a structural homolog, YiiX (PDB 2if6) identified a fatty acid in the conserved hydrophobic pocket, thus providing additional insights into possible function of these novel enzymes.

59 BASIC BIOLOGICAL SCIENCES↗

Structural analysis of activating mutants of YfiB from Pseudomonas aeruginosa PAO1

Highlights: • Crystal structures of activating mutants of P. aeruginosa YfiB were determined. • F48S and W55L activating mutants of YfiB crystallize as dimers. • The L43P mutant of YfiB is monomeric and forms a stable complex with YfiR. • The YfiB activating region undergoes a dramatic rearrangement upon YfiR binding. • Extensive interactions are formed between the YfiB activating region and YfiR. Bacterial cyclic-di-GMP (c-di-GMP) is an important messenger molecule that influences diverse cellular processes including motility, virulence and cytotoxicity systems, polysaccharide synthesis and biofilm formation. The YfiBNR tripartite signalling system in P. aeruginosa modulates the cellular c-di-GMP levels in response to signals received from the periplasm. In this study, we analyse the structures of activating mutants of the outer membrane protein YfiB that give rise to increased surface attachment and biofilm formation. The F48S and W55L mutants of YfiB(27–168) crystallize in the same dimeric arrangement as our previously reported YfiB structures that preclude complex formation with YfiR. The L43P mutant of YfiB(27–168) is monomeric and forms a stable complex with YfiR. The YfiB(L43P)-YfiR crystal structure reveals a dramatic rearrangement of the N-terminal fragment, which is implicated in increased YfiB activation and membrane attachment, upon YfiR binding. Comparison with our previous complex structure between YfiB(59–168) and YfiR reveals extensive interactions between the N-terminal fragment of YfiB (residues 35–55) and YfiR.

60 APPLIED LIFE SCIENCES↗

Key Material Properties for Thermo-Structural Analysis of Transformational Challenge Reactor Core Components

This report presents thermo-structural (Williamson 2012) constitutive relationships and models pertaining to Transformational Challenge Reactor (TCR) core components using BISON. The material correlations for TCR core candidate materials include 316L stainless steel, uranium dioxide, and Chemical Vapor Infiltrated (CVI) silicon carbide were compiled. Preliminary, sensitivity analyses were performed on a simple cylindrical geometry by parametrically varying material properties. The sensitivity analyses elucidate the impact of material properties on the induced stresses in the TCR core structures. The results of the sensitivity studies for the SiC concept showed that the swelling is expected to be the largest source of uncertainty, followed by the elastic modulus. In the SS316L and UO 2 concept, the thermal conductivity of the can was the dominate effect, and a temperature dependent correlation or tabulated data would be advisable. The geometry and modeling method simulations showed that the inclusion of the heat generation is the SiC can does alter the stress and temperature magnitudes. However, the stress concentrations and profiles remain consistent.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

Computer program performs stiffness matrix structural analysis

Computer program generates the stiffness matrix for a particular type of structure from geometrical data, and performs static and normal mode analyses. It requires the structure to be modeled as a stable framework of uniform, weightless members, and joints at which loads are applied and weights are lumped.

Bamford, R.↗

A method of limit point calculation in finite element structural analysis

An approach is presented for the calculation of limit points for structures described by discrete coordinates, and whose governing equations derive from finite element concepts. The nonlinear load-displacement path of the imperfect structure is first traced by use of a direct iteration scheme and the determinant of the governing algebraic equations is calculated at each solution point. The limit point is then established by extrapolation and imposition of the condition of zero slope of the plot of load vs. determinant. Three problems are solved in illustration of the approach and in comparison with alternative procedures and test data.

Gallagher, R. H.↗

A fuselage/tank structure study for actively cooled hypersonic cruise vehicles: Structural analysis

The effects of fuselage cross-section (circular and elliptical) and structural arrangement (integral and nonintegral tanks) on the performance of actively cooled hypersonic cruise vehicles was evaluated. It was found that integrally machined stiffening of the tank walls, while providing the most weight-efficient use of materials, results in higher production costs. Fatigue and fracture mechanics appeared to have little effect on the weight of the three study aircraft. The need for thermal strain relief through insulation is discussed. Aircraft size and magnitude of the internal pressure are seen to be significant factors in tank design.

Baker, A. H.↗

A family of solution algorithms for nonlinear structural analysis based on relaxation equations

A family of hierarchical algorithms for nonlinear structural equations are presented. The algorithms are based on the Davidenko-Branin type homotopy and shown to yield consistent hierarchical perturbation equations. The algorithms appear to be particularly suitable to problems involving bifurcation and limit point calculations. An important by-product of the algorithms is that it provides a systematic and economical means for computing the stepsize at each iteration stage when a Newton-like method is employed to solve the systems of equations. Some sample problems are provided to illustrate the characteristics of the algorithms.

Park, K. C.↗

Probabilistic structural analysis methods and applications

An advanced algorithm for simulating the probabilistic distribution of structural responses due to statistical uncertainties in loads, geometry, material properties, and boundary conditions is reported. The method effectively combines an advanced algorithm for calculating probability levels for multivariate problems (fast probability integration) together with a general-purpose finite-element code for stress, vibration, and buckling analysis. Application is made to a space propulsion system turbine blade for which the geometry and material properties are treated as random variables.

Cruse, T. A.↗

Structural analysis of cell wall polysaccharides using PACE

The plant cell wall is composed of many complex polysaccharides. The composition and structure of the polysaccharides affect various cell properties including cell shape, cell function and cell adhesion. Many techniques to characterize polysaccharide structure are complicated, requiring expensive equipment and specialized operators e.g. NMR, MALDI-MS. PACE (Polysaccharide Analysis using Carbohydrate gel Electrophoresis) uses a simple, rapid technique to analyze polysaccharide quantity and structure (Goubet et al. 2002). Whilst the method here describes xylan analysis, it can be applied (by use of the appropriate glycosyl hydrolase) to any cell wall polysaccharide.

59 BASIC BIOLOGICAL SCIENCES↗

Preliminary Nonlinear Structural Analysis of Advanced Composite Tow-Steered Shells with Large Geometric Imperfections

The structural performance of two advanced composite tow-steered shells with and without tow overlaps, and with large geometric imperfections, are predicted using linear and geometrically nonlinear finite element analyses. These shells, 35 in. long and approximately 16.3 in. diameter, are fabricated using automated fiber placement from IM7/8552 graphite/epoxy prepreg. The 8-ply,[±45/±Θ]s. shell layup incorporates a steered fiber angle Θ that varies from 10 deg. to 45 deg. periodically over the shell circumference. Shell analysis models are evaluated using geometric imperfections normalized to ±1 shell wall thickness (±0.040 in.), which are then superposed and rotated incrementally around the shell longitudinal axis. Using these nominal imperfections, the shell prebuckling axial stiffnesses and buckling loads predicted with linear and nonlinear analyses are close to reference values from linear analyses with no imperfections. The linear and nonlinear analyses are then repeated for scaled imperfections that are larger by up to a factor of 10. For these larger imperfections, linear analyses predict reductions in axial stiffnesses and buckling loads of up to 5 and 30 percent, respectively, from reference values. The nonlinear analyses predict even larger reductions in axial stiffnesses and buckling loads of up to 10 and 55 percent, respectively.

Dobrin, Calvin P.↗

Assessment of Technologies for the Space Shuttle External Tank Thermal Protection System and Recommendations for Technology Improvement: Structural Analysis Technologies and Modeling Practices - Part 2

A technology review and assessment of modeling and analysis efforts underway in support of a safe return to flight of the thermal protection system (TPS) for the Space Shuttle external tank (ET) are summarized. This review and assessment effort focuses on the structural modeling and analysis practices employed for ET TPS foam design and analysis and on identifying analysis capabilities needed in the short-term and long-term. The current understanding of the relationship between complex flight environments and ET TPS foam failure modes are reviewed as they relate to modeling and analysis. A literature review on modeling and analysis of TPS foam material systems is also presented. Finally, a review of modeling and analysis tools employed in the Space Shuttle Program is presented for the ET TPS acreage and close-out foam regions. This review includes existing simplified engineering analysis tools are well as finite element analysis procedures.

Knight, Norman F., Jr.↗

Assessment of Technologies for the Space Shuttle External Tank Thermal Protection System and Recommendations for Technology Improvement: Structural Analysis Technologies and Modeling Practices - Part 2

A technology review and assessment of modeling and analysis efforts underway in support of a safe return to flight of the thermal protection system (TPS) for the Space Shuttle external tank (ET) are summarized. This review and assessment effort focuses on the structural modeling and analysis practices employed for ET TPS foam design and analysis and on identifying analysis capabilities needed in the short-term and long-term. The current understanding of the relationship between complex flight environments and ET TPS foam failure modes are reviewed as they relate to modeling and analysis. A literature review on modeling and analysis of TPS foam material systems is also presented. Finally, a review of modeling and analysis tools employed in the Space Shuttle Program is presented for the ET TPS acreage and close-out foam regions. This review includes existing simplified engineering analysis tools are well as finite element analysis procedures.

Knight, Norman F., Jr.↗

Structural Analysis Report for Sandia High Altitude Aerosol Research (SHAAR) Payloads

Sandia National Laboratories has interest in mounting enclosures to gather scientific data aboard NASA scientific balloons. This report documents the structural integrity of three separate payloads considered ‘piggybacks.’ To date, there are no design criteria for piggybacks, therefore each piggyback shall follow the same design requirements set by NASA per the Gondola Structural Design Requirements. This analysis report shall describe how each payload meets the design requirements laid out in the Gondola Design Requirements.

47 OTHER INSTRUMENTATION↗