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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 397 records · Page 22

Structure and immune recognition of trimeric pre-fusion HIV-1 Env

The human immunodeficiency virus type 1 (HIV-1) envelope (Env) spike, comprising three gp120 and three gp41 subunits, is a conformational machine that facilitates HIV-1 entry by rearranging from a mature unliganded state, through receptor-bound intermediates, to a post-fusion state. As the sole viral antigen on the HIV-1 virion surface, Env is both the target of neutralizing antibodies and a focus of vaccine efforts. Here we report the structure at 3.5 Å resolution for an HIV-1 Env trimer captured in a mature closed state by antibodies PGT122 and 35O22. This structure reveals the pre-fusion conformation of gp41, indicates rearrangements needed for fusion activation, and defines parameters of immune evasion and immune recognition. Pre-fusion gp41 encircles amino- and carboxy-terminal strands of gp120 with four helices that form a membrane-proximal collar, fastened by insertion of a fusion peptide-proximal methionine into a gp41-tryptophan clasp. Spike rearrangements required for entry involve opening the clasp and expelling the termini. In conclusion, N-linked glycosylation and sequence-variable regions cover the pre-fusion closed spike; we used chronic cohorts to map the prevalence and location of effective HIV-1-neutralizing responses, which were distinguished by their recognition of N-linked glycan and tolerance for epitope-sequence variation.

59 BASIC BIOLOGICAL SCIENCES↗

Dynamic enhancer landscapes in human craniofacial development

The genetic basis of human facial variation and craniofacial birth defects remains poorly understood. Distant-acting transcriptional enhancers control the fine-tuned spatiotemporal expression of genes during critical stages of craniofacial development. However, a lack of accurate maps of the genomic locations and cell type-resolved activities of craniofacial enhancers prevents their systematic exploration in human genetics studies. Here, we combine histone modification, chromatin accessibility, and gene expression profiling of human craniofacial development with single-cell analyses of the developing mouse face to define the regulatory landscape of facial development at tissue- and single cell-resolution. We provide temporal activity profiles for 14,000 human developmental craniofacial enhancers. We find that 56% of human craniofacial enhancers share chromatin accessibility in the mouse and we provide cell population- and embryonic stage-resolved predictions of their in vivo activity. Taken together, our data provide an expansive resource for genetic and developmental studies of human craniofacial development.

60 APPLIED LIFE SCIENCES↗

Three-dimensional photoluminescence imaging of threading dislocations in GaN by sub-band optical excitation

GaN is rapidly gaining attention for implementation in power electronics but is still impacted by its high density of threading dislocations (TDs), which have been shown to facilitate current leakage through devices limiting their performance and reliability. Here, we discuss a novel implementation of photoluminescence (PL) imaging to study TDs in regions within vertically structured p-i-n GaN (PIN) diodes consisting of metalorganic chemical vapor deposition (MOCVD) epitaxial layers grown on ammonothermal GaN (am-GaN) substrates. PL imaging with a sub-bandgap excitation energy (3.1 eV) reveals TDs with excellent clarity in three dimensions within the am-GaN substrate. Galvanometric-driven PL imaging allows the microstructure of hundreds of devices to be characterized in a single session, enhancing the screening process through the addition of device specific TD location tracking and density mapping. The visibility, structural characteristics, luminescent nature and evolution of TDs through the GaN growth process are described, potentially providing the ability to define TD structures associated with leakage current.

36 MATERIALS SCIENCE↗

Ultrafast Relativistic Electron Nanoprobes

Abstract One of the frontiers in electron scattering is to couple ultrafast temporal resolution with highly localized probes to investigate the role of microstructure on material properties. Here, taking advantage of the high average brightness of our electron source, we demonstrate the generation of ultrafast relativistic electron beams with picometer-scale emittance and their ability to probe nanoscale features on materials with complex microstructures. The electron beam is tightly focused at the sample plane by a custom in-vacuum lens system, and its evolution around the waist is accurately reconstructed. We then use the focused beam to characterize a Ti-6 wt% Al polycrystalline sample by correlating the diffraction and imaging modality, showcasing the capability to locate grain boundaries and map adjacent crystallographic domains with sub-micron precision. This work provides a paradigm for ultrafast electron instrumentation, enabling characterization techniques such as relativistic ultrafast electron nano-diffraction and ultrafast scanning transmission electron microscopy.

77 NANOSCIENCE AND NANOTECHNOLOGY↗

Processing ultrasonic inspection data from multiple scan patterns for turbine rotor weld build-up evaluations

The study presents a data processing methodology for weld build-up using multiple scan patterns. To achieve an overall high probability of detection for flaws with different orientations, an inspection procedure with three different scan patterns is proposed. The three scan patterns are radial-tangential longitude wave pattern, axial-radial longitude wave pattern, and tangential shear wave pattern. Scientific fusion of the inspection data is implemented using volume reconstruction techniques. The idea is to perform spatial domain forward data mapping for all sampling points. A conservative scheme is employed to handle the case that multiple sampling points are mapped to one grid location. The scheme assigns the maximum value for the grid location to retain the largest equivalent reflector size for the location. The methodology is demonstrated and validated using a realistic ring of weld build-up. Tungsten balls and bars are embedded to the weld build-up during manufacturing process to represent natural flaws. Flat bottomed holes and side drilled holes are installed as artificial flaws. Automatic flaw identification and extraction are demonstrated. Results indicate the inspection procedure with multiple scan patterns can identify all the artificial and natural flaws.

42 ENGINEERING↗

Detection of quasar feedback from the thermal Sunyaev–zel’dovich effect in Planck

Poorly understood feedback processes associated with highly luminous black hole accretion in quasars may dramatically affect the properties of their host galaxies. We search for the effect of quasar feedback on surrounding gas using Planck maps of the thermal Sunyaev–Zel’dovich effect (tSZ). By stacking tSZ Compton-y maps centered on the locations of 26,686 spectroscopic quasars from the Sloan Digital Sky Survey, we detect a strong but unresolved tSZ Compton-y signal at >5σ significance that likely originates from a combination of virialized halo atmosphere gas and quasar feedback effects. We show that the feedback contribution to our detected quasar tSZ signal is likely to dominate over virialized halo gas by isolating the feedback tSZ component for high- and low-redshift quasars. We find that this quasar tSZ signal also scales with black hole mass and bolometric luminosity, all consistent with general expectations of quasar feedback. We estimate the mean angularly integrated Compton-y of quasars at z ≃ 1.5 to be 3.5 × 10{sup −6} Mpc{sup 2}, corresponding to mean total thermal energies in feedback and virialized halo gas of 1.1(±0.2)×10{sup 62} erg, and discuss the implications for quasar feedback. If confirmed, the large total thermal feedback energetics we estimate to be 5% (±1% statistical uncertainty) of the black hole mass will have important implications for the effects of quasar feedback on the host galaxy, as well as the surrounding intergalactic medium.

79 ASTRONOMY AND ASTROPHYSICS↗

Halometry from astrometry

Halometry—mapping out the spectrum, location, and kinematics of nonluminous structures inside the Galactic halo—can be realized via variable weak gravitational lensing of the apparent motions of stars and other luminous background sources. Additionally, modern astrometric surveys provide unprecedented positional precision along with a leap in the number of cataloged objects. Astrometry thus offers a new and sensitive probe of collapsed dark matter structures over a wide mass range, from one millionth to several million solar masses. It opens up a window into the spectrum of primordial curvature fluctuations with comoving wavenumbers between 5 Mpc -1 and 10 5 Mpc -1 , scales hitherto poorly constrained. We outline detection strategies based on three classes of observables—multi-blips, templates, and correlations—that take advantage of correlated effects in the motion of many background light sources that are produced through time-domain gravitational lensing. While existing techniques based on single-source observables such as outliers and mono-blips are best suited for point-like lens targets, our methods offer parametric improvements for extended lens targets such as dark matter subhalos. Lastly, multi-blip lensing events may also unveil the existence, location, and mass of planets in the outer reaches of the Solar System, where they would likely have escaped detection by direct imaging.

79 ASTRONOMY AND ASTROPHYSICS↗

NEWTS Database Dashboard

The NEWTS (National Energy Water Treatment and Speciation) database dashboard displays sites across the nation where energy-related wastewater stream samples and composition data have been collected. Using the dashboard, community leaders and the public will be able to quickly explore and obtain sample data from locations displayed on a map where various government agencies collect and analyze water samples from energy-related wastewater streams.

Aqueous Chemistry↗

Carbon Storage Atlas (Fifth Edition)

The Carbon Storage Atlas contains the following sections: (1) Introduction to CCS; (2) DOE’s Carbon Storage Activities; (3) National Perspectives; (4) Large-Scale Field Projects; (5) Small-Scale Field Projects; and (6) American Recovery and Reinvestment Act (ARRA) Site Characterization Projects. The Introduction to CCS section is an overview of CCS. The DOE’s Carbon Storage Activities section is a summary of CCS activities including information on DOE’s Carbon Capture and Storage Programs, NETL’s Research and Development, DOE’s Systems Analysis Activities, and DOE’s Interagency and Global Collaborations, and Knowledge Sharing Efforts. The National Perspectives section contains maps showing the number, location, and magnitude of CO 2 stationary sources in the United States and other portions of North America, as well as the areal extent and estimated CO 2 prospective storage resource available in RCSP-evaluated geologic formations. The Large-Scale Field Projects section provides detailed information on various aspects of the large-scale injections conducted by the RCSPs. The Small-Scale Field Projects and Site Characterization Projects sections provide summaries of field project activities that augment the efforts of the large-scale field projects.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Carbon Storage Atlas, Fifth Edition - Updated Information on Geological Sinks and CO 2 Sources

The Carbon Storage Atlas contains the following sections: (1) Introduction to CCS; (2) DOE’s Carbon Storage Activities; (3) National Perspectives; (4) Large-Scale Field Projects; (5) Small-Scale Field Projects; and (6) American Recovery and Reinvestment Act (ARRA) Site Characterization Projects. The Introduction to CCS section is an overview of CCS. The DOE’s Carbon Storage Activities section is a summary of CCS activities including information on DOE’s Carbon Capture and Storage Programs, NETL’s Research and Development, DOE’s Systems Analysis Activities, and DOE’s Interagency and Global Collaborations, and Knowledge Sharing Efforts. The National Perspectives section contains maps showing the number, location, and magnitude of CO 2 stationary sources in the United States and other portions of North America, as well as the areal extent and estimated CO 2 prospective storage resource available in RCSP-evaluated geologic formations. The Large-Scale Field Projects section provides detailed information on various aspects of the large-scale injections conducted by the RCSPs. The Small-Scale Field Projects and Site Characterization Projects sections provide summaries of field project activities that augment the efforts of the large-scale field projects.

54 ENVIRONMENTAL SCIENCES↗

Red Hills Storage Hub Feasibility Study - Summary of Milestone 2 - Conceptual Basis of Plants and Pipeline Infrastructure

This document provides the first conceptual-level design of plants and pipeline infrastructure, including maps showing the project location, the potential footprint of the carbon capture and storage plant, the potential site of the capture and compression unit, and the conceptual pipeline layout for transporting the CO2 product from the capture facility to the future potential injection sites.

Carbon Capture and Storage,CarbonSAFE↗

A distributed temperature profiling method for assessing spatial variability in ground temperatures in a discontinuous permafrost region of Alaska

Soil temperature has been recognized as a property that strongly influences a myriad of hydro-biogeochemical processes and reflects how various properties modulate the soil thermal flux. In spite of its importance, our ability to acquire soil temperature data with high spatial and temporal resolution and coverage is limited because of the high cost of equipment, the difficulties of deployment, and the complexities of data management. Here we propose a new strategy that we call distributed temperature profiling (DTP) for improving the characterization and monitoring near-surface thermal properties through the use of an unprecedented number of laterally and vertically distributed temperature measurements. We developed a prototype DTP system, which consists of inexpensive, low-impact, low-power, and vertically resolved temperature probes that independently and autonomously record soil temperature. The DTP system concept was tested by moving sequentially the system across the landscape to identify near-surface permafrost distribution in a discontinuous permafrost environment near Nome, Alaska, during the summertime. Results show that the DTP system enabled successful acquisition of vertically resolved profiles of summer soil temperature over the top 0.8 m at numerous locations. DTP also enabled high-resolution identification and lateral delineation of near-surface permafrost locations from surrounding zones with no permafrost or deep permafrost table locations overlain by a perennially thawed layer. The DTP strategy overcomes some of the limitations associated with – and complements the strengths of – borehole-based soil temperature sensing as well as fiber-optic distributed temperature sensing (FO-DTS) approaches. Combining DTP data with co-located topographic and vegetation maps obtained using unmanned aerial vehicle (UAV) and electrical resistivity tomography (ERT) data allowed us to identify correspondences between surface and subsurface property distribution and in particular between topography, vegetation, shallow soil properties, and near-surface permafrost. Finally, the results highlight the considerable value of the newly developed DTP strategy for investigating the significant variability in and complexity of subsurface thermal and hydrological regimes in discontinuous permafrost regions.

54 ENVIRONMENTAL SCIENCES↗

Orthoimagery and Shapefiles Documenting Pre- and Post-August 2019 Slope Disturbances, Teller Road Site, Seward Peninsula, Alaska, 2018-2019

This dataset was derived from UAS aerial photos and dGPS data collected at the Teller mile marker 47 site in 2018 and 2019 and used to support research quantifying the timing and rate of surface movements and analyze soil transport process in Arctic landscapes. In July 2018, a Phantom uncrewed aerial system (UAS) collected >6800 high resolution aerial photos of the lower portion of the Teller 47 watershed (see for raw photos; pending archive NGA281). During the UAS survey, 25 x 25 cm tile ground control points (GCPs) were laid out and secured with one rebar rod in the center. The four corners and rebar tops were surveyed with differential GPS, and these coordinates were used to construct and validate an orthomosaic of the site. These points were resurveyed in August 2019 and used to track annual movement. Changes in position between the two surveys are reported in (Lathrop et al. 2022; NGA254). Agisoft Metashape photogrammetry software was used to construct a georeferenced orthomosaic image (*.tif file) of a portion (0.68 km2) of the watershed with a final resolution of ~1 cm. Manual delineation of the perimeters of failures visible in the UAS imagery (*.tif files) was conducted to create shapefile polygons (two *.zip files). The failures were identified by the exposure of bare mineral soils, which were made visible by disruption of the overlying tundra vegetation. The shapefiles were then used to analyse the topographic distribution and sizes of the failures. In August 2019 an additional ~300 georeferenced UAS images of slope instability features were collected (data pending submission) and compared with the 2018 orthomosaic. Overview maps of the failure locations included as a *.pdf.The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska.Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

A technique for interpretation of multispectral remote sensor data

The author has identified the following significant results. The U.S. Army Engineer Waterways Experiment Station is engaged in a study to detect from ERTS-1 satellite data alterations to the absorption and scattering properties caused by movement of suspended particles and solutes in selected areas of the Chesapeake Bay and to correlate the data to determine the feasibility of delineating flow patterns, flushing action of the estuary, and sediment and pollutant dispersion. As a part of this study, ADP techniques have been developed that permit automatic interpretation of data from any multispectral remote sensor with computer systems which have limited memory capacity and computing speed. The multispectral remote sensor is considered as a reflectance spectrophotometer. The data which define the spectral reflectance characteristics of a scene are scanned pixel by pixel. Each pixel whose spectral reflectance matches a reference spectrum is identified, and the results are shown in a map that identifies the locations where spectrum matches were detected and spectrum that was matched. The interpretation technique is described and an example of interpreted data from ERTS-1 is presented.

Williamson, A. N.↗

Dynamics of plankton populations in upwelling areas

The author has identified the following significant results. Spectral properties of the upwelled waters off the NW coast of Africa were studied with observations derived from aircraft and Skylab. Results indicate that the two-channel, ratio approach is ineffective in determining surface chlorophyll concentrations. Ocean color boundaries and temperature gradients were found to be directly correlated with each other and also with fishing effort in the upwelling region. Photographic and scanner data derived from Skylab were effective in locating ocean boundaries and mapping temperature distributions.

Szekielda, K. H.↗

Meteorological interpretations of the images from the Nimbus 5 electrically scanned microwave radiometer

The Nimbus 5 Electrically Scanned Microwave Radiometer (ESMR) is useful in determining the extent, structure, and intensity of rainfall over oceans. It is possible on the basis of rainfall maps to determine the location of frontal rain, rain/snow boundaries, and the structure of tropical storms. ESMR data on rainfall over the east coast of the United States and the Gulf of Mexico, on snowfall over the northeast coast of the United States, on frontal zones in the northwestern Pacific and on the tropical storm Leila in the South Indian Ocean are examined. Because of the generally high emissivities of land surfaces, interpretation of meteorological parameters over land is not yet possible.

Wilheit, T. T.↗