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At least 397 records · Page 22

Deep learning models for interpretation of point of care ultrasound in military working dogs

Introduction: Military working dogs (MWDs) are essential for military operations in a wide range of missions. With this pivotal role, MWDs can become casualties requiring specialized veterinary care that may not always be available far forward on the battlefield. Some injuries such as pneumothorax, hemothorax, or abdominal hemorrhage can be diagnosed using point of care ultrasound (POCUS) such as the Global FAST® exam. This presents a unique opportunity for artificial intelligence (AI) to aid in the interpretation of ultrasound images. In this article, deep learning classification neural networks were developed for POCUS assessment in MWDs. Methods: Images were collected in five MWDs under general anesthesia or deep sedation for all scan points in the Global FAST® exam. For representative injuries, a cadaver model was used from which positive and negative injury images were captured. A total of 327 ultrasound clips were captured and split across scan points for training three different AI network architectures: MobileNetV2, DarkNet-19, and ShrapML. Gradient class activation mapping (GradCAM) overlays were generated for representative images to better explain AI predictions. Results: Performance of AI models reached over 82% accuracy for all scan points. The model with the highest performance was trained with the MobileNetV2 network for the cystocolic scan point achieving 99.8% accuracy. Across all trained networks the diaphragmatic hepatorenal scan point had the best overall performance. However, GradCAM overlays showed that the models with highest accuracy, like MobileNetV2, were not always identifying relevant features. Conversely, the GradCAM heatmaps for ShrapML show general agreement with regions most indicative of fluid accumulation. Discussion: Overall, the AI models developed can automate POCUS predictions in MWDs. Preliminarily, ShrapML had the strongest performance and prediction rate paired with accurately tracking fluid accumulation sites, making it the most suitable option for eventual real-time deployment with ultrasound systems. Further integration of this technology with imaging technologies will expand use of POCUS-based triage of MWDs.

59 BASIC BIOLOGICAL SCIENCES↗

Performance and Total Cost of Ownership of a Fuel Cell Hybrid Mining Truck

The main objective of this work was to investigate the potential of hydrogen and fuel cells replacing diesel and internal combustion engines in the ultraclass haul trucks deployed in the mining sector. Performance, range, durability, and cost are the main criteria considered for comparing the two fuels and engine options. Fuel cell system (FCS) performance is characterized in terms of heat rejection, efficiency, and fuel consumption for a hybrid platform equivalent to a 3500 hp diesel engine operating on a representative open pit mining duty cycle. A hybrid platform was chosen because the heat rejection, with a constrained radiator frontal area, limits the maximum fuel cell-rated power by about 50% compared to that of the diesel truck. The hybrid powertrain was 81–88% more efficient than the diesel powertrain on the truck duty cycle. A liquid hydrogen storage system is required for an equal range or time between refilling, but the packaging remains a challenge. Fuel cell and battery durability were evaluated for their performance degradation and lifetime. Achieving a fuel cell lifetime comparable to the time between major overhauls for diesel trucks necessitates the oversizing of the membrane-active area, catalyst overloading, and voltage clipping. For an equal lifetime, the battery must be oversized to control its depth of discharge and charge/discharge rates. A total cost of ownership (TCO) analysis considering the initial capital expenditures, as well as the lifetime cost of fuel, operation, and maintenance, indicates that fuel cells and hydrogen can compete with diesel. A breakeven fuel cost for TCO parity is obtained if H2 is available at USD 5.79–6.85/kg vs. diesel at USD 3.25/gal and the FCS-specific cost is USD 323/kW e relative to USD 250/kW for a diesel genset. Volume manufacturing is required for FCS cost reduction. High volume is possible through the standardization, modularity, and proliferation of class 8 long-haul truck systems across different heavy-duty applications.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Characterization of Local and Systemic Impact of Whitefly (Bemisia tabaci) Feeding and Whitefly-Transmitted Tomato Mottle Virus Infection on Tomato Leaves by Comprehensive Proteomics

Tomato mottle virus (ToMoV) is a ssDNA plant begomovirus transmitted to solanaceous crops by the whitefly species complex (Bemisia tabaci), causing stunted growth, leaf mottling, and reduced fruit yield. Using a genetic repertoire of 7 genes, ToMoV pathogenesis includes manipulation of multiple plant biological processes to circumvent antiviral defenses. To further understand the effects of whitefly feeding and whitefly-transmitted ToMoV infection on tomato plants (Solanum lycopersicum ‘Florida Lanai’), we generated comprehensive protein profiles of leaves subjected to feeding by either (1) viruliferous whiteflies harboring ToMoV, or (2) non-viruliferous whiteflies, or (3) a no-feeding control. In each condition (1-3), whiteflies were confined to clip cages on a mature leaf for 3 days. The effects of whitefly feeding and ToMoV infection were measured both locally and systemically by sampling either a mature leaf directly from the site of whitefly feeding, or from a newly formed leaf 10 days post feeding (dpf). At 3 dpf, relatively few biological processes were affected specifically by ToMoV at the site of feeding, and include proteins associated with translation initiation and elongation, and plasmodesmata dynamics. In contrast, the systemic impacts of ToMoV on younger leaves at 10 dpf were more pronounced and included a virus-specific change in plant proteins associated with mRNA maturation and export, RNA-dependent DNA methylation, and other plant anti-viral transcriptional gene silencing systems. Our analysis supports previous findings and provides novel insight into biological processes in tomato altered locally and systemically by whitefly feeding and ToMoV infection.

59 BASIC BIOLOGICAL SCIENCES↗

Quasi-Distributed Fiber Sensor-Based Approach for Pipeline Health Monitoring: Generating and Analyzing Physics-Based Simulation Datasets for Classification

This study presents a framework for detecting mechanical damage in pipelines, focusing on generating simulated data and sampling to emulate distributed acoustic sensing (DAS) system responses. The workflow transforms simulated ultrasonic guided wave (UGW) responses into DAS or quasi-DAS system responses to create a physically robust dataset for pipeline event classification, including welds, clips, and corrosion defects. This investigation examines the effects of sensing systems and noise on classification performance, emphasizing the importance of selecting the appropriate sensing system for a specific application. The framework shows the robustness of different sensor number deployments to experimentally relevant noise levels, demonstrating its applicability in real-world scenarios where noise is present. Overall, this study contributes to the development of a more reliable and effective method for detecting mechanical damage to pipelines by emphasizing the generation and utilization of simulated DAS system responses for pipeline classification efforts. The results on the effects of sensing systems and noise on classification performance further enhance the robustness and reliability of the framework.

36 MATERIALS SCIENCE↗

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Histones belong to a family of highly conserved proteins in eukaryotes. They pack DNA into nucleosomes as functional units of chromatin. Post-translational modifications (PTMs) of histones, which are highly dynamic and can be added or removed by enzymes, play critical roles in regulating gene expression. In plants, epigenetic factors including histone PTMs are related to their adaptive responses to the environment. Understanding the molecular mechanisms of epigenetic control can bring unprecedented opportunities for engineering solutions to increase the resilience of crops to climate change. Herein, we describe a protocol to isolate the nuclei and purify histones from sorghum leaf tissue. The extracted histones can be analyzed as their intact forms by top-down mass spectrometry (MS) coupled to online reversed-phase (RP) liquid chromatography (LC). Combinations and stoichiometry of multiple PTMs on the same histone proteoform can be readily identified. In addition, histone tail clipping can be detected using the top-down LC-MS workflow thus yielding the global PTM profile of core histones (H4, H2A, H2B, H3). By comparing the PTM profile among samples corresponding to different conditions (e.g. drought vs. control), potential epigenetic marks can be discovered as targets for further characterization using approaches such as chromatin immunoprecipitation – sequencing (ChIP-seq).

59 BASIC BIOLOGICAL SCIENCES↗

Reconnaissance with JWST of the J-region Asymptotic Giant Branch in Distance Ladder Galaxies: From Irregular Luminosity Functions to Approximation of the Hubble Constant

Abstract We study stars in the J-regions of the asymptotic giant branch (JAGB) of near-infrared color–magnitude diagrams in the maser host NGC 4258 and four hosts of six Type Ia supernovae (SNe Ia): NGC 1448, NGC 1559, NGC 5584, and NGC 5643. These clumps of stars are readily apparent near 1.0 < F150W − F277W < 1.5 andm F150W = 22–25 mag with James Webb Space Telescope NIRCam photometry. Various methods have been proposed to assign an apparent reference magnitude to this recently proposed standard candle, including the mode, median, sigma-clipped mean, or a modeled luminosity function parameter. We test the consistency of these by measuring intrahost variations, finding differences of up to ∼0.2 mag that significantly exceed statistical uncertainties. Brightness differences appear intrinsic, and are further amplified by the nonuniform shape of the JAGB luminosity function, also apparent in the LMC and SMC. We follow a “many methods” approach to measure consistently JAGB magnitudes and distance moduli to the SN Ia host sample calibrated by NGC 4258. We find broad agreement with distance moduli measured from Cepheids, tip of the red giant branch, and Miras. However, the SN host mean distance modulus estimated via the JAGB method necessary to estimateH 0 differs by ∼0.19 mag among the above definitions, the result of different levels of luminosity function asymmetry. The methods yield a full range of 71−78 km s −1 Mpc −1 , i.e., a fiducial result ofH 0 = 74.7 ± 2.1(stat) ± 2.3(sys, ±3.1 if combined in quadrature) km s −1 Mpc −1 , with systematic errors limited by the differences in methods. Future work may seek to standardize and refine this promising tool further, making it more competitive with established distance indicators.

Astronomy & Astrophysics↗

47 Tuc in Rubin Data Preview 1. Exploring Early LSST Data and Science Potential

We present analyses of the early data from Rubin Observatory’s Data Preview 1 (DP1) for the field of the globular cluster 47 Tuc. The DP1 data set for 47 Tuc includes four nights of observations from the Rubin Commissioning Camera (LSSTComCam), covering multiple bands (ugriy). We address challenges of crowding in the inner region of the cluster and toward the SMC in DP1, and demonstrate improved star–galaxy separation by fitting fifth-degree polynomials to the stellar loci in color–color diagrams and applying multidimensional sigma clipping. We compile a catalog of 3576 probable 47 Tuc member stars selected via a combination of isochrone, Gaia proper-motion, and color–color space matched filtering. We explore the sources of photometric scatter in the 47 Tuc color–color sequence, evaluating contributions from various potential sources, including differential extinction within the cluster. Finally, of the 72 well-characterized variables in the field, we recover three known variable stars, including two RR Lyrae and one eclipsing binary, in the coadd-based object catalog, and identify 62 in the difference image-based object catalog. Although the DP1 lightcurves have sparse temporal sampling, they appear to follow the patterns of densely sampled literature lightcurves well. Despite some data limitations for crowded-field stellar analysis, DP1 demonstrates the promising scientific potential for future LSST data releases.

Choi, Yumi [NSF National Optical-Infrared Astronom↗

Fixture aids soldering of electronic components on circuit board

Spring clamp fixture holds small electronic components in a desired position while they are being soldered on a circuit board. The spring clamp is clipped on the edge of the circuit board and an adjustable spring-steel boom holds components against the board. The felt pad at the end of the boom is replaced with different attachments for other holding tasks.

Ross, M. H.↗

High mobility work station restraint support

Chair holds man in semistanding posture enabling astronauts to work comfortably with minimum restriction in weightless environment. Seat, angled at 130 deg to back support, twists and swivels up to 20 deg in all directions but forward. Two flexible thigh clips prevent occupant from slipping off.

Schermerhorn, R. S.↗

Inertial waste separation system for zero G WMS

The design, operation, and flight test are presented for an inertial waste separation system. Training personnel to use this system under simulated conditions is also discussed. Conclusions indicate that before the system is usable in zero gravity environments, a mirror for the user's guidance should be installed, the bounce cycle and bag changing system should be redesigned, and flange clips should be added to improve the user's balance.

Source record↗

Manufacture, assembly, and delivery of beryllium test panels

Details of the fabrication and assembly of two 99 cm by 99 cm (39 in. by 39 in.) beryllium heat shield test panels are presented. Each panel consists of two hat-stiffened, formed skins which overlap a transverse slip joint at the center of the panel; clips join the two skins at the slip joint, and continuous standoffs are provided at the ends of the panel. The hat-stiffeners are joined to the skin by furnace-brazing, using the braze alloy BAg 18. The parts are generally 0.051 cm (0.020 in.) thick. Tools used to form the detail parts are shown, together with the results of preliminary forming and brazing trials to verify processing techniques. Problems encountered in the manufacture of the panels are discussed.

Source record↗

Aerodynamic stability and control characteristics of TBC shuttle booster AR-11981-3

A scale model of the Boeing Company space shuttle booster configuration 3 was tested in the MSFC 14-inch trisonic wind tunnel. This test was proposed to fill-in the original test run schedule as well as to investigate the aerodynamic stability and control characteristics of the booster with three wing configurations not previously tested. The configurations tested included: (1) a cylindrical booster body with an axisymmetric nose, (2) clipped delta canards that had variable incidence from 0 deg to -60 deg, (3) different aft body mounted wing configurations, (4) two vertical fin configurations, and (5) a Grumman G-3 orbiter configuration. Tests were conducted over a Mach range from 0.6 to 5.0.

Phelps, E. R.↗

A simplified correlation technique for position location using earth satellites.

To derive range to a moving vehicle for surveillance and navigation purposes, the phase delays in sets of sinusoidal tones may be measured using synchronous satellites for signal relay. When the number of users is large, problems of rapid acquisition and measurement occur in multiple-access systems. This paper describes the selection and processing of an orthogonal tone set which has several useful features. The paper also presents a simulation of a simplified digital technique for the noise-free case, as well as for a composite signal buried in wideband noise. The digital simulation is also modified to include the proposed 'hard limiting' or clipping technique.

Martin, S. C.↗

Evidence for a metabolic limitation of survival in hypothermic hamsters.

The underlying factors limiting survival in the hypothermic state are studied. Hamsters of both sexes, clipped and unclipped, were inducted into profound hypothermia by the helium cold method until they reached a temperature between 7 and 10 C. It appears that the primary cause of death is failure of respiration due to the depletion of carbohydrate energy supplies and may explain why survival time in hypothermia is shorter than the normal hibernation time of the hamster.

Prewitt, R. L.↗

Subcritical crack growth of selected aerospace pressure vessel materials

This experimental program was undertaken to determine the effects of combined cyclic/sustained loads, stress level, and crack shape on the fatigue crack growth rate behavior of cracks subjected to plane strain conditions. Material/environment combinations tested included: 2219-T87 aluminum plate in gaseous helium, room air, and 3.5% NaCl solution at room temperature, liquid nitrogen, and liquid hydrogen; 5Al-2.5 Sn (ELI) titanium plate in liquid nitrogen and liquid hydrogen and 6AL-4V (ELI) STA titanium plate in gaseous helium and methanol at room temperature. Most testing was accomplished using surface flawed specimens instrumented with a clip gage to continuously monitor crack opening displacements at the specimen surface. Tapered double cantilever beam specimens were also tested. Static fracture and ten hour sustained load tests were conducted to determine fracture toughness and apparent threshold stress intensity values. Cyclic tests were performed using sinusoidal loading profiles at 333 MHz (20 cpm) and trapezoidal loading profiles at both 8.3 MHz (0.5 cpm) and 3.3 MHz (0.2 cpm). Data were evaluated using modified linear elastic fracture mechanics parameters.

Hall, L. R.↗

Apollo 15 mission. Temporary loss of command module television picture

An investigation was made into the temporary loss of command module color television picture by the ground station converter at Mission Control Center. Results show the picture loss was caused by a false synchronization pulse that resulted from the inability of the black level clipping circuit to respond adequately to the video signal when bright sunlight suddenly entered the camera's field of view.

Source record↗

Wind-tunnel roll-damping measurements of a winged space shuttle configuration in launch attitude

Ground-wind load studies were conducted on three model configurations to assess the importance of aeroelastic instabilities of erected space shuttle vehicles. Roll damping was measured on a fuselage-alone model, which had a D cross section, and a fuselage and tail surfaces in combination with either a clipped-delta wing or a low-sweep tapered wing as the primary lifting surface. The largest negative roll-damping coefficients were measured with the fuselage-alone configuration and were a function of wind azimuth. At the wind azimuths at which the wing-fuselage configuration was unstable, the negative roll-damping coefficients were a function of reduced frequency.

Hess, R. W.↗