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At least 397 records · Page 22

Growth hormone aggregates in the rat adenohypophysis

Although it has been known for some time that GH aggregates are contained within the rat anterior pituitary gland, the role that they might play in pituitary function is unknown. The present study examines this issue using the technique of Western blotting, which permitted visualization of 11 GH variants with apparent mol wt ranging from 14-88K. Electroelution of the higher mol wt variants from gels followed by their chemical reduction with beta-mercaptoethanol increased GH immunoassayability by about 5-fold. With the blot procedure we found 1) that GH aggregates greater than 44K were associated with a 40,000 x g sedimentable fraction; 2) that GH aggregates were not present in glands from thyroidectomized rats, but were in glands from the thyroidectomized rats injected with T4; 3) that GH aggregates were uniquely associated with a heavily granulated somatotroph subpopulation isolated by density gradient centrifugation; and 4) that high mol wt GH forms were released from the dense somatotrophs in culture, since treatment of the culture medium with beta-mercaptoethanol increased GH immunoassayability by about 5-fold. Taken together, the results show that high mol wt GH aggregates are contained in secretory granules of certain somatotrophs and are also released in aggregate form from these cells in vitro.

NASA Discipline Cell Biology↗

Shoshone River Water Resources: Assessing Sediment Inputs into the Shoshone River in Wyoming to Determine Areas for Protection and Restoration Practices

In 2016, a routine repair operation at the Willwood Dam released tons of built-up sediment into the Shoshone River, polluting the river and negatively impacting the ecosystem. This release greatly affected the communities that rely on the river for farming, recreation, and tourism. In partnership with the Wyoming Department of Environmental Quality (WYDEQ), Shoshone River Partners, and the United States Geological Survey (USGS) Wyoming–Montana Water Science Center, this project utilized satellite imagery and precipitation data to examine turbidity patterns in the Shoshone River between the Buffalo Bill Dam and the Willwood Dam. We used PlanetScope satellite images to assess changes in surface reflectance of the river in response to precipitation events and Global Precipitation Measurement (GPM) Integrated Multi-Spectral Retrieval (IMERG) precipitation data to estimate the lag time between rainfall events and increased turbidity. The National Land Cover Dataset (2019) was used to identify the main land cover types within each sub-basin. The end products included a turbidity analysis, land cover analysis, and precipitation analysis that provided the partners with a better understanding of sediment dynamics in the river. The results demonstrated the feasibility of using PlanetScope data to examine turbidity spatially along small rivers. Sediment plumes from tributaries were visually identified for multiple high turbidity events, and we calibrated an equation that translated reflectance to turbidity, accurately representing plume extent. Inconsistent spectral quality of PlanetScope data, however, limited our ability to assess the relative sediment contribution of the tributaries.

tributaries↗

Visual Analytics of Multivariate Networks With Representation Learning and Composite Variable Construction

Multivariate networks are commonly found in real-world data-driven applications. Uncovering and understanding the relations of interest in multivariate networks is not a trivial task. This article presents a visual analytics workflow for studying multivariate networks to extract associations between different structural and semantic characteristics of the networks (e.g., what are the combinations of attributes largely relating to the density of a social network?). The workflow consists of a neural-network-based learning phase to classify the data based on the chosen input and output attributes, a dimensionality reduction and optimization phase to produce a simplified set of results for examination, and finally an interpreting phase conducted by the user through an interactive visualization interface. A key part of our design is a composite variable construction step that remodels nonlinear features obtained by neural networks into linear features that are intuitive to interpret. We demonstrate the capabilities of this workflow with multiple case studies on networks derived from social media usage and also evaluate the workflow with qualitative feedback from experts.

97 MATHEMATICS AND COMPUTING↗

NASA’s Moon Trek Portal: New Capabilities Supporting Mission Planning and Engagement

Introduction: NASA’s Moon Trek (https://trek.nasa.gov/moon/) is one of a growing number of interactive, browser-based, online portals for planetary data visualization and analysis produced by NASA’s Solar System Treks Project (SSTP). Moon Trek continues to be enhanced with new data and new capabilities enabling it to facilitate the planning and conducting of upcoming lunar missions by NASA, its commercial partners, and its international partners, as well as advancing its role as a valuable outreach tool. A Comprehensive Online Web Portal: Developed at NASA’s Jet Propulsion Laboratory (JPL) and managed as a project of NASA’s Solar System Exploration Research Virtual Institute (SSERVI) at NASA Ames Research Center, Moon Trek is a browser-based web portal. The portal provides easy-to-use tools for browsing, data layering, data product blending, and feature search among thousands of data products covering topography, mineralogy, elemental abundance, geology, and much more. Visualizations are provided in var-ious map projections, interactive 3D viewing, and in virtual reality. Using an in-house stereo workflow, SSTP is able to produce new NAC-based high-resolution mosaics and DEMs. Diverse Applications for Lunar Exploration: Baseline analytic tools available to all users include dis-tance measurement, elevation profiling, sun angle calculation, and 3D print file generation. More advanced account-level tools allow users to perform more computationally intensive analyses. These include ray-traced lighting analysis for user-specified areas over user-specified time/date ranges and time intervals, electro-static surface potential analysis, subsetting of large data products, slope analysis, and Lunar Laser Ranging geometry calculation. Artificial intelligence (AI) and ma-chine learning (ML) based tools have been implemented for crater detection and hazard analysis, boulder detection and hazard analysis, and rockfall detection. New Tools Facilitating Exploration: Additional, new tools have recently been added and others are in development, offering even greater functionality in con-ducting analyses of potential landing sites and areas of surface operations. The new Line-of-Sight tool facilitates communications planning between locations on the lunar surface, between any given site on the lunar surface and a specified ground station on the Earth, and between a site on the lunar surface and a relay asset in lunar orbit, all taking into account local lunar topography. The new Data Plotter tool provides both tabular and graphical representations of pixel values along a user specified path for a growing number of data products. The new NAC Finder tool will identify and pro-vide access to NAC images that intersect a user-defined path or bounded area. The SSTP development team is looking to leverage the capabilities of its existing AI and ML crater, boulder, and rockfall detection and analysis tools, and extend that technology to a generalized feature detector that can be trained on instances of specific types of landforms and then search the lunar surface for more examples of such features. New traverse planning tools are being developed with use cases in generalized concept studies and specific mission planning in mind. These will facilitate finding optimal traverse paths based on constraints such as slope, lighting, hazard avoidance, and communications. These will be complemented by new traverse visualization capabilities. Users will be able to interactively ride along with a rover, examining 3D views of the terrain while adjusting camera height and viewing angle along with selecting different data layer overlays to drape across the terrain. Engaging the Public: The capabilities being developed for mission planning are being leveraged to further enhance Moon Trek’s proven utility as a valuable public outreach resource. This includes providing multiple lev-els of engagement with different points of entry. At its simplest level, promoting understanding through visualization, media and the public will be able to easily visualize and conduct their own exploration of lunar sites targeted by NASA and its partners. For a more in-depth experience, we are working with our stakeholders to promote understanding through interaction by extend-ing our current landing site and traverse analysis capabilities, making simplified access to these tools available to those who want to explore more deeply key factors in planning a mission through interactive and possibly even gamified experiences. The highest degree of public outreach, focusing on engagement through scientific participation, could be achieved through our work with missions and the NASA Office of the Chief Scientist on Moon Trek’s extension as a tool with specialized capabilities for facilitating citizen science. In such scenarios, participants become members of extended mis-sion science teams, using dedicated and integrated interfaces to analyze mission data to help answer questions key to lunar science and exploration. We are work-ing with NASA’s Office of Communications, museums, planetariums, and the media to help them easily integrate accurate, detailed visualizations of NASA’s lunar destinations and exploration into their content/productions and to engage diverse audiences in diverse venues.

Moon Trek↗

Retinal ganglion cell projections to the hamster suprachiasmatic nucleus, intergeniculate leaflet, and visual midbrain: bifurcation and melanopsin immunoreactivity

The circadian clock in the suprachiasmatic nucleus (SCN) receives direct retinal input via the retinohypothalamic tract (RHT), and the retinal ganglion cells contributing to this projection may be specialized with respect to direct regulation of the circadian clock. However, some ganglion cells forming the RHT bifurcate, sending axon collaterals to the intergeniculate leaflet (IGL) through which light has secondary access to the circadian clock. The present studies provide a more extensive examination of ganglion cell bifurcation and evaluate whether ganglion cells projecting to several subcortical visual nuclei contain melanopsin, a putative ganglion cell photopigment. The results showed that retinal ganglion cells projecting to the SCN send collaterals to the IGL, olivary pretectal nucleus, and superior colliculus, among other places. Melanopsin-immunoreactive (IR) ganglion cells are present in the hamster retina, and some of these cells project to the SCN, IGL, olivary pretectal nucleus, or superior colliculus. Triple-label analysis showed that melanopsin-IR cells bifurcate and project bilaterally to each SCN, but not to the other visual nuclei evaluated. The melanopsin-IR cells have photoreceptive characteristics optimal for circadian rhythm regulation. However, the presence of moderately widespread bifurcation among ganglion cells projecting to the SCN, and projection by melanopsin-IR cells to locations distinct from the SCN and without known rhythm function, suggest that this ganglion cell type is generalized, rather than specialized, with respect to the conveyance of photic information to the brain. Copyright 2003 Wiley-Liss, Inc.

NASA Discipline Neuroscience↗

Effect of Target Location on Dynamic Visual Acuity During Passive Horizontal Rotation

The vestibulo-ocular reflex (VOR) generates eye rotation to compensate for potential retinal slip in the specific plane of head movement. Dynamic visual acuity (DVA) has been utilized as a functional measure of the VOR. The purpose of this study was to examine changes in accuracy and reaction time when performing a DVA task with targets offset from the plane of rotation, e.g. offset vertically during horizontal rotation. Visual acuity was measured in 12 healthy subjects as they moved a hand-held joystick to indicate the orientation of a computer-generated Landolt C "as quickly and accurately as possible." Acuity thresholds were established with optotypes presented centrally on a wall-mounted LCD screen at 1.3 m distance, first without motion (static condition) and then while oscillating at 0.8 Hz (DVA, peak velocity 60 deg/s). The effect of target location was then measured during horizontal rotation with the optotypes randomly presented in one of nine different locations on the screen (offset up to 10 deg). The optotype size (logMar 0, 0.2 or 0.4, corresponding to Snellen range 20/20 to 20/50) and presentation duration (150, 300 and 450 ms) were counter-balanced across five trials, each utilizing horizontal rotation at 0.8 Hz. Dynamic acuity was reduced relative to static acuity in 7 of 12 subjects by one step size. During the random target trials, both accuracy and reaction time improved proportional to optotype size. Accuracy and reaction time also improved between 150 ms and 300 ms presentation durations. The main finding was that both accuracy and reaction time varied as a function of target location, with greater performance decrements when acquiring vertical targets. We conclude that dynamic visual acuity varies with target location, with acuity optimized for targets in the plane of motion. Both reaction time and accuracy are functionally relevant DVA parameters of VOR function.

Appelbaum, Meghan↗

2022 Prentice Award Lecture: Advancing Retinal Imaging and Visual Function in Patient Management and Disease Mechanisms

Patient-based research plays a key role in probing basic visual mechanisms. Less-well recognized is the role of patient-based retinal imaging and visual function studies in elucidating disease mechanisms, which are accelerated by advances in imaging and function techniques and are most powerful when combined with the results from histology and animal models. A patient's visual complaints can be one key to patient management, but human data are also key to understanding disease mechanisms. Unfortunately, pathological changes can be difficult to detect. Before advanced retinal imaging, the measurement of visual function indicated the presence of pathological changes that were undetectable with existing clinical examination. Over the past few decades, advances in retinal imaging have increasingly revealed the unseen. This has led to great strides in the management of many diseases, particularly diabetic retinopathy and macular edema, and age-related macular degeneration. It is likely widely accepted that patient-based research, as in clinical trials, led to such positive outcomes. Both visual function measures and advanced retinal imaging have clearly demonstrated differences among retinal diseases. Contrary to initial thinking, sight-threatening damage in diabetes occurs to the outer retina and not only to the inner retina. This has been clearly indicated in patient results but has only gradually entered the clinical classifications and understanding of disease etiology. There is strikingly different pathophysiology for age-related macular degeneration compared with photoreceptor and retinal pigment epithelial genetic defects, yet research models and even some treatments confuse these. It is important to recognize the role that patient-based research plays in probing basic visual mechanisms and elucidating disease mechanisms, combining these findings with the concepts from histology and animal models. Thus, this article combines sample instrumentation from my laboratory and progress in the fields of retinal imaging and visual function.

60 APPLIED LIFE SCIENCES↗

In-Flight Flow Visualization Using Infrared Thermography

The feasibility of remote infrared thermography of aircraft surfaces during flight to visualize the extent of laminar flow on a target aircraft has been examined. In general, it was determined that such thermograms can be taken successfully using an existing airplane/thermography system (NASA Dryden's F-18 with infrared imaging pod) and that the transition pattern and, thus, the extent of laminar flow can be extracted from these thermograms. Depending on the in-flight distance between the F-18 and the target aircraft, the thermograms can have a spatial resolution of as little as 0.1 inches. The field of view provided by the present remote system is superior to that of prior stationary infrared thermography systems mounted in the fuselage or vertical tail of a subject aircraft. An additional advantage of the present experimental technique is that the target aircraft requires no or minimal modifications. An image processing procedure was developed which improves the signal-to-noise ratio of the thermograms. Problems encountered during the analog recording of the thermograms (banding of video images) made it impossible to evaluate the adequacy of the present imaging system and image processing procedure to detect transition on untreated metal surfaces. The high reflectance, high thermal difussivity, and low emittance of metal surfaces tend to degrade the images to an extent that it is very difficult to extract transition information from them. The application of a thin (0.005 inches) self-adhesive insulating film to the surface is shown to solve this problem satisfactorily. In addition to the problem of infrared based transition detection on untreated metal surfaces, future flight tests will also concentrate on the visualization of other flow phenomena such as flow separation and reattachment.

vanDam, C. P.↗

Visual Systems Mapping to Define and Compare Woody Biomass LCAs for Sustainable Systems

The challenge addressed in this research centres on the need to choose between several biomass sources and energy production processes, while supporting rural economies and resilience of forest systems. A key barrier to effective decision-making for strategies using biomass is the lack of standardized and transparent life cycle assessment (LCA) baselines. These baselines are critical for assessing the impacts of biomass strategies but often vary due to regional factors and chosen simplifying assumptions of the LCAs. However, omitting key variables can mean the LCA omits key feedback and balancing loops relevant to fully assessing impacts of the change or test scenario. To address these complexities, this project employs a systems engineering approach: visual systems mapping. This technique is used to define the boundaries and dynamic behaviours of LCA baselines, enhancing transparency. By examining five literature sources and their documented baseline scenarios, the systems mapping case-studies demonstrates an approach to documenting and archiving these baselines. Recommendations are that visual systems mapping should be used to document key assumptions, such as baselines, of LCAs. Further, where possible open data repositories should hold key information about LCA baselines and reproducible workflows (e.g., using open-source tools) should be used to improve transparency and comparability in LCAs. Given the consensus within the broader scientific community on the importance of replicable data practices, this research reinforces the need for standardized frameworks and systems engineering tools in LCAs. This research demonstrates a pathway to more transparent, standardized, and comparable LCAs, that may bolster decisions for biomass systems.

Davis, Maggie [ORNL] (ORCID:0000000181319328)↗

A User-Centered Design Study in Scientific Visualization Targeting Domain Experts

The development of usable visualization solutions is essential for ensuring both their adoption and effectiveness. User-centered design principles, which involve users throughout the entire development process, have been shown to be effective in numerous information visualization endeavors. We describe how we applied these principles in scientific visualization over a two year collaboration to develop a hybrid in situ/post hoc solution tailored towards combustion researcher needs. Lastly, we examine the importance of user-centered design and lessons learned over the design process in an effort to aid others seeking to develop effective scientific visualization solutions.

97 MATHEMATICS AND COMPUTING↗

Choosing Your Poison: Optimizing Simulator Visual System Selection as a Function of Operational Tasks

Although current technology simulator visual systems can achieve extremely realistic levels they do not completely replicate the experience of a pilot sitting in the cockpit, looking at the outside world. Some differences in experience are due to visual artifacts, or perceptual features that would not be present in a naturally viewed scene. Others are due to features that are missing from the simulated scene. In this paper, these differences will be defined and discussed. The significance of these differences will be examined as a function of several particular operational tasks. A framework to facilitate the choice of visual system characteristics based on operational task requirements will be proposed.

Sweet, Barbara T.↗

The development and use of metal-based probes for X-ray fluorescence microscopy

Abstract X-ray fluorescence microscopy (XFM) has become a widely used technique for imaging the concentration and distribution of metal ions in cells and tissues. Recent advances in synchrotron sources, optics, and detectors have improved the spatial resolution of the technique to <10 nm with attogram detection sensitivity. However, to make XFM most beneficial for bioimaging—especially at the nanoscale—the metal ion distribution must be visualized within the subcellular context of the cell. Over the years, a number of approaches have been taken to develop X-ray-sensitive tags that permit the visualization of specific organelles or proteins using XFM. In this review, we examine the types of X-ray fluorophore used, including nanomaterials and metal ions, and the approaches used to incorporate the metal into their target binding site via antibodies, genetically encoded metal-binding peptides, affinity labeling, or cell-specific peptides. We evaluate their advantages and disadvantages, review the scientific findings, and discuss the needs for future development.

36 MATERIALS SCIENCE↗

Technical keynote address on remote sensing

A review of remote sensing techniques is presented. Various types of remote sensors are described and the platforms used to mount the sensors are examined. Examples of remote sensing by aerial photography in infrared, ultraviolet, and visual spectra are included. The types of equipment are designated and their specific areas of application are defined. It is concluded that the primary objective of remote sensing is to contribute to man's ability to manage and use the terrestrial environment.

Holter, M. R.↗

Ozone in the upper troposphere from Gasp measurements

Several aspects of tropospheric ozone variations are examined by using ozone measurements made from commercial airliners (Gasp data). Through visual inspection of the autocorrelation function it is shown that the east-west variations of ozone have a predominant wavelength near 2400 km, while temperature and wind have predominant wavelengths near 3300 km. Distance-lagged correlation functions of ozone with temperature and wind show a definite periodicity with wavelengths near 2400 km. Attention is given to the tropical tropospheric ozone values above 100 parts per billion by volume, which appear to be associated with meridional transport from middle latitudes, and in some cases, relatively large tropical ozone values are coincident with clouds.

Nastrom, G. D.↗

Motion sickness in the squirrel monkey

In this study of susceptibility to motion sickness the specific aims were to examine the effects of combined vertical rotation and horizontal acceleration, phenotype, sex, visual cues, morning and afternoon testing, and repeated test exposures on incidence, frequency, and latency of emetic responses. The highest emetic incidence of 89% with an emetic frequency of 2.0 during 60 min and a latency of 19 min from onset of testing occurred at 25 rpm and 0.5 Hz linear acceleration. Since the emetic responses were quite similar to man in eliciting motion stimuli it was concluded that the squirrel monkey represents a very suitable primate model for studies of motion and space sickness.

Ordy, J. M.↗

Holocinematographic Velocimeter

Holographic movies used to study turbulent flows. High-speed movie composed of single-exposure holograms of tracer particles in each of two simultaneous orthogonal views of flow. Time resolution set by movie frame rate, and total time limited by length of movie. Data from system used to verify results of theoretical models and numerical simulations of turbulence, visualize and measure coherent structures in "non-simple" turbulent flows, to examine mechanisms operative in various turbulence-control/drag-reduction concepts.

Weinstein, Leonard M.↗

Perceiving environmental structure from optical motion

Generally speaking, one of the most important sources of optical information about environmental structure is known to be the deforming optical patterns produced by the movements of the observer (pilot) or environmental objects. As an observer moves through a rigid environment, the projected optical patterns of environmental objects are systematically transformed according to their orientations and positions in 3D space relative to those of the observer. The detailed characteristics of these deforming optical patterns carry information about the 3D structure of the objects and about their locations and orientations relative to those of the observer. The specific geometrical properties of moving images that may constitute visually detected information about the shapes and locations of environmental objects is examined.

Lappin, Joseph S.↗

Science information systems: Visualization

Future programs in earth science, planetary science, and astrophysics will involve complex instruments that produce data at unprecedented rates and volumes. Current methods for data display, exploration, and discovery are inadequate. Visualization technology offers a means for the user to comprehend, explore, and examine complex data sets. The goal of this program is to increase the effectiveness and efficiency of scientists in extracting scientific information from large volumes of instrument data.

Wall, Ray J.↗