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At least 397 records · Page 22

Universally high transcript error rates in bacteria

Errors can occur at any level during the replication and transcription of genetic information. Genetic mutations derived mainly from replication errors have been extensively studied. However, fundamental details of transcript errors, such as their rate, molecular spectrum, and functional effects, remain largely unknown. To globally identify transcript errors, we applied an adapted rolling-circle sequencing approach to Escherichia coli, Bacillus subtilis, Agrobacterium tumefaciens, and Mesoplasma florum, revealing transcript-error rates 3 to 4 orders of magnitude higher than the corresponding genetic mutation rates. The majority of detected errors would result in amino-acid changes, if translated. With errors identified from 9929 loci, the molecular spectrum and distribution of errors were uncovered in great detail. A G→A substitution bias was observed in M. florum, which apparently has an error-prone RNA polymerase. Surprisingly, an increased frequency of nonsense errors towards the 3' end of mRNAs was observed, suggesting a Nonsense-Mediated Decay-like quality-control mechanism in prokaryotes.

59 BASIC BIOLOGICAL SCIENCES↗

Precipitation observed by the airborne advanced microwave moisture sounder (AMMS)

Airborne radiometric measurements at frequencies near 92 GHz and 183 GHz were conducted over two precipitation events near Wallops Island, Virginia during February, 1986. The measured brightness temperatures are compared with those from calculations to estimate the snowfall and rainfall rates for both events. The estimated rates over water surface are within a factor of two of those derived from the concurrent measurements by the SPANDAR radar at the NASA Wallops Flight Facility. These estimated rates, however, suggest that both snowfall events are light and close to the threshold of radiometric detection especially over land surface. Observations of additional snowfall events with lower frequency channels are needed to demonstrate the approach and to account for cloud effects.

Chang, A. T. C.↗

Analysis of ionospheric refraction error corrections for GRARR systems

A determination is presented of the ionospheric refraction correction requirements for the Goddard range and range rate (GRARR) S-band, modified S-band, very high frequency (VHF), and modified VHF systems. The relation ships within these four systems are analyzed to show that the refraction corrections are the same for all four systems and to clarify the group and phase nature of these corrections. The analysis is simplified by recognizing that the range rate is equivalent to a carrier phase range change measurement. The equation for the range errors are given.

Mallinckrodt, A. J.↗

A simulation investigation of the effects of engine-and thrust-response characteristics on helicopter handling qualities

A multi-phase program is being conducted to study, in a generic sense and through ground simulation, the effects of engine response, rotor inertia, rpm control, excess power, and vertical damping on specific maneuvers included in nap-of-the-Earth (NOE) operations. The helicopter configuration with an rpm-governed gas-turbine engine are considered. Handling-qualities-criteria data are considered in light of aspects peculiar to rotary-wing and NOE operations. The results of three moving-based piloted simulation studies are summarized and the frequency, characteristics of the helicopter thrust response which set it apart from other VTOL types are explained. Power-system response is affected by both the engine-governor response and the level of rotor inertia. However, results indicate that with unlimited power, variations in engine response can have a significant effect on pilot rating, whereas changes in rotor inertia, in general, do not. The results also show that any pilot interaction required to maintain proper control can significantly degrade handling qualities. Data for variations in vertical damping and collective sensitivity are compared with existing handling-qualities specifications, MIL-F-83300 and AGARD 577, and show a need for higher minimums for both damping and sensitivity for the bob-up task. Results for cases of limited power are also shown.

Corless, L. D.↗

Experimental feasibility of investigating acoustic waves in Couette flow with entropy and pressure gradients

The feasibility is discussed for an experimental program for studying the behavior of acoustic wave propagation in the presence of strong gradients of pressure, temperature, and flow. Theory suggests that gradients effects can be experimentally observed as resonant frequency shifts and mode shape changes in a waveguide. A convenient experimental geometry for such experiments is the annular region between two co-rotating cylinders. Radial temperature gradients in a spinning annulus can be generated by differentially heating the two cylinders via electromagnetic induction. Radial pressure gradients can be controlled by varying the cylinder spin rates. Present technology appears adequate to construct an apparatus to allow independent control of temperature and pressure gradients. A complicating feature of a more advanced experiment, involving flow gradients, is the requirement for independently controlled cylinder spin rates. Also, the boundary condition at annulus terminations must be such that flow gradients are minimally disturbed. The design and construction of an advanced apparatus to include flow gradients will require additional technology development.

Parrott, Tony L.↗

Modal Propellant Gauging: High-resolution and non-invasive gauging of both settled and unsettled liquids in reduced gravity.

The modal response of a liquid-filled tank to external acoustic excitation can be used to infer with high resolutionthe mass of contained liquid, the mass flow rate of liquids into and out of the tank, and changes in tank pressure.Both contained liquid mass and internal ullage pressure affect the modal response of the tank walls through fluidmass-loading of the tank walls and pressure-induced wall stiffening, respectively. Modal Propellant Gauging refersto the technology that exploits these shifts in modal frequencies to infer the mass of propellant in a tank. MPG is anon-invasive gauging technology that has demonstrated gauging resolutions of 1% for settled propellants and 2-3%for unsettled, sloshing propellants. Extensive parabolic flight testing of the MPG system on model tanks has beenconducted to validate the technology in reduced gravity. MPG testing on a qualification tank for the Orion Program'sEuropean Service Module has also been conducted and is reported here. Finite element modeling of the OrionESM "upper" tank is discussed and compared with measurement data. Three computational approaches to massdetermination, Peak Tracking, Point Sensor, and Spectral Density methods, are described here. Use cases are definedand analyzed in the context of the Orion ESM Qualification tank data, and an implementation scheme for continuousmass gauging on the Orion ESM is discussed.Keywords: Propellant Mass Gauging, Modal Analysis, Orion ESM

Propellant Mass Gauging↗

Temporal Patterns in Soil Redox Potential Vary Across a Freshwater Coastal Delta

Widespread and persistent flooding is submerging coastal ecosystems, particularly along the Louisiana Gulf coast, where flooding results from the compounding effects of ground subsidence and rising sea level. Restoration projects aim to mitigate land loss by diverting sediment loads from rivers into degraded areas to increase ground elevation. To predict how coastal ecosystems will change over time in response to projected changes in relative sea level and restoration, it is necessary to understand how subsurface biogeochemical processes respond to dynamic hydrologic forcings. Here, this study evaluates how environmental parameters that integrate biogeochemical processes vary with water table fluctuations in the freshwater Wax Lake Delta (WLD) in Louisiana, USA, where water diversions have formed one of the only active deltas along the coast. High‐frequency observations of water level, soil redox potential, specific conductance and pH were made for 1 year along elevation transects located on the older, proximal and younger, distal ends of a deltaic island. Redox responded rapidly to changing water tables, with fluctuations occurring primarily in shallow soils (< 20 cm) and at higher elevations. Deeper soils and those at lower elevation remained inundated and reduced. Semi‐diurnal tidal fluctuations were pronounced in younger, distal soils, presumably due to rapid groundwater exchange with the river channel. Tidal signals were muted in older soils that instead exhibited seasonal variability associated with river discharge and evapotranspiration. Although much of the delta sediments are persistently reducing and anoxic, redox fluctuations in the natural levees that border the deltaic islands likely drive high rates of biogeochemical activity. Evaluating how hydrology drives the frequency and duration of redox fluctuations provides a basis for understanding how biogeochemical processes might vary with complex hydrological interactions in coastal systems.

Herndon, Elizabeth [Oak Ridge National Laboratory ↗

eCoral: How Electrolysis Could Restore Seawater Conditions Ideal for Coral Reefs

Coral reefs suffer from climate change, including long-term ocean acidification (OA) and warming and short-term bleaching, tropical storms, and pollution events, all of which are increasing in frequency and severity. It is urgent yet unclear how to intervene to save coral reefs. Reversal of the ocean pH to preindustrial levels could restore coral reefs to their preindustrial growth rates; however, strategies to reverse OA on environmentally relevant scales have not been established. Anecdotally, electrolysis seems to help coral reefs recover from acidification and short-term events, but few uncontrolled studies support such claims. Here, using two independent continuum simulation approaches (COMSOL and CrunchFlow), we show the effect of electrolysis on seawater chemistry relevant to coral reef survival and growth. We conclude that near the negative electrodes, the cathodes, seawater pH, supersaturation, and carbonate concentration all increase significantly. Electrolysis of seawater, therefore, can be used to restore preindustrial ocean conditions locally to save coral reefs, an approach termed eCoral here. We anticipate these simulation results to be the starting point for controlled experiments to test whether seawater electrolysis promotes coral reef growth and restoration, as these simulations predict.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

The effect of blue and infrared laser melting frequency on oxide morphology in 304L

Continuous Wave Laser Beam (LB) melting offers control over the localised heating and cooling of melt pools for welding, brazing, additive manufacturing and solidification. Research into laserliquid metal interactions has primarily focused on the heat and mass transport under large thermal gradients imposed by the localised melting conditions. Recent commercial availability of blue (450 nm) wavelength lasers has enabled the printing of material with poor IR (1064) nm attenuation like Cu. However, little research has been conducted into varying input laser frequency and scan rate at fixed absorption to understand the effects of laser light on surface oxide formation and the underlying liquid metal thermodynamic response. Here, this article conducts laser melting of 304L under Blue (450 nm) and Infrared (IR, 1064 nm) laser frequencies and examines their impact on laser oxide thickness, chemistry and coloration. We find that laser frequencies induce changes in the oxide layer thickness and chemistry that cannot be explained using conventional thermal absorption shifts and fluid dynamics. We suggest that light coupling may have thermodynamic implications for the chemical potential of the liquid metal, which may drive the observed phase behaviour. Identifying mechanisms for the tuning of the chemical potential via laser frequency control could enable the ability to control solidification microstructure and atomic ordering in laser welding and additive manufacturing processes.

304L↗

The effects of aircraft design and atmospheric turbulence on handling and ride qualities

The effects of aircraft dynamic characteristics on passenger ride quality were investigated to determine ride-quality isocontours similar to aircraft handling-qualities contours. Measurements were made on a motion-base simulator while varying the aircraft short-period and Dutch Roll frequencies and dampings. Both pilot ratings and subjective ride-quality ratings were obtained during simulator flight. Ride and handling qualities were found to be complimentary for the Dutch Roll mode, but not for the short-period mode. Regions of optimal ride and handling qualities were defined for the short-period mode, and the effects of changes in turbulence level studied using mathematical models.

Jones, C. R.↗

The effect of water vapor on fatigue crack Growth in 7475-t651 aluminum alloy plate

The effects of water vapor on fatigue crack growth in 7475-T651 aluminum alloy plate at frequencies of 1 Hz and 10 Hz were investigated. Twenty-five mm thick compact specimens were subjected to constant amplitude fatigue testing at a load ratio of 0.2. Fatigue crack growth rates were calculated from effective crack lengths determined using a compliance method. Tests were conducted in hard vacuum and at water vapor partial pressures ranging from 94 Pa to 3.8 kPa. Fatigue crack growth rates were frequency insensitive under all environment conditions tested. For constant stress intensity factor ranges crack growth rate transitions occurred at low and high water vapor pressures. Crack growth rates at intermediate pressures were relatively constant and showed reasonable agreement with published data for two Al-Cu-Mg alloys. The existence of two crack growth rate transitions suggests either a change in rate controlling kinetics or a change in corrosion fatigue mechanism as a function of water vapor pressure. Reduced residual deformation and transverse cracking specimens tested in water vapor versus vacuum may be evidence of embrittlement within the plastic zone due to environmental interaction.

Dicus, D. L.↗

Effect of water vapor on fatigue crack growth in 7475-T651 aluminum alloy plate

The effects of water vapor on fatigue crack growth in 7475-T651 aluminum alloy plate at frequencies of 1 Hz and 10 Hz were investigated. Twenty-five mm thick compact specimens were subjected to constant amplitude fatigue testing at a load ratio of 0.2. Fatigue crack growth rates were calculated from effective crack lengths determined using a compliance method. Tests were conducted in hard vacuum and at water vapor partial pressures ranging from 94 Pa to 3.8 kPa. Fatigue crack growth rates were frequency insensitive under all environment conditions tested. For constant stress intensity factor ranges crack growth rate transitions occurred at low and high water vapor pressures. Crack growth rates at intermediate pressures were relatively constant and showed reasonable agreement with published data for two Al-Cu-Mg alloys. The existence of two crack growth rate transitions suggests either a change in rate controlling kinetics or a change in corrosion fatigue mechanism as a function of water vapor pressure. Reduced residual deformation and transverse cracking specimens tested in water vapor versus vacuum may be evidence of embrittlement within the plastic zone due to environmental interaction.

Dicus, D. L.↗

A Study of the Mass Loss Rates of Symbiotic Star Systems

The amount of mass loss in symbiotic systems is investigated, specifically mass loss via the formation of jets in R Aquarii (R Aqr). The jets in R Aqr have been observed in the X-ray by Chandra over a four year time period. The jet changes on times scales of a year and new outflows have been observed. Understanding the amount of mass and the frequency of ejection further constrain the ability of the white dwarf in the system to accrete enough mass to become a Type la supernova progenitor. The details of multi-wavelength studies, such as speed, density and spatial extent of the jets will be discussed in order to understand the mass balance in the binary system. We examine other symbiotic systems to determine trends in mass loss in this class of objects.

Korreck, K. E.↗

Wide Tuning Capability for Spacecraft Transponders

A document presents additional information on the means of implementing a capability for wide tuning of microwave receiver and transmitter frequencies in the development reported in the immediately preceding article, VCO PLL Frequency Synthesizers for Spacecraft Transponders (NPO- 42909). The reference frequency for a PLL-based frequency synthesizer is derived from a numerically controlled oscillator (NCO) implemented in digital logic, such that almost any reference frequency can be derived from a fixed crystal reference oscillator with microhertz precision. The frequency of the NCO is adjusted to track the received signal, then used to create another NCO frequency used to synthesize the transmitted signal coherent with, and at a specified frequency ratio to, the received signal. The frequencies can be changed, even during operation, through suitable digital programming. The NCOs and the related tracking loops and coherent turnaround logic are implemented in a field-programmable gate array (FPGA). The interface between the analog microwave receiver and transmitter circuits and the FPGA includes analog-to-digital and digital-toanalog converters, the sampling rates of which are chosen to minimize spurious signals and otherwise optimize performance. Several mixers and filters are used to properly route various signals.

Lux, James↗

Optical Properties of Ar Ions Irradiated Nanocrystalline ZrC and ZrN Thin Films

Thin nanocrystalline ZrC and ZrN films (less than 400 nanometers), grown on (100) Si substrates at a substrate temperature of 500 degrees Centigrade by the pulsed laser deposition (PLD) technique, were irradiated by 800 kiloelectronvolts Ar ion irradiation with fluences from 1 times 10(sup 14) atoms per square centimeter up to 2 times 10(sup 15) atoms per square centimeter. Optical reflectance data, acquired from as-deposited and irradiated films, in the range of 500-50000 per centimeter (0.06–6 electronvolts), was used to assess the effect of irradiation on the optical and electronic properties. Both in ZrC and ZrN films we observed that irradiation affects the optical properties of the films mostly at low frequencies, which is dominated by the free carriers response. In both materials, we found a significant reduction in the free carriers scattering rate, i.e. possible increase in mobility, at higher irradiation flux. This is consistent with our previous findings that irradiation affects the crystallite size and the micro-strain, but it does not induce major structural changes.

ZrN↗

AGR-3/4 TRISO Fuel Compact Ceramography

The combined third and fourth irradiation in the Advanced Gas Reactor (AGR) program (AGR-3/4) contained tristructural isotropic (TRISO)-coated particle fuel and designed-to-fail (DTF) fuel particles. The DTF particles were only coated with highly anisotropic pyrocarbon (PyC) so they would purposely fail during the AGR-3/4 irradiation and provide a source of fission products for measurement. To observe the post-irradiation morphology of these DTF particles and the TRISO-coated particles, three AGR-3/4 compacts were mounted in epoxy, sectioned above their centerlines, and ground/polished. Three rounds of grinding/polishing and optical microscopy were performed so that the particles could be observed at multiple planes. Each compact contained approximately 1,872 TRISO particles and exactly 20 DTF particles. The three AGR-3/4 compacts examined covered a wide temperature range and featured both the hottest average irradiation temperature (1375°C) and the coldest/lowest burnup (872°C and 5.5% fissions per initial metal atom) of any compacts to undergo post-irradiation examination (PIE) in the AGR program to date. A total of 29 DTF particles were located and observed via microscopy across the three compacts. All the DTF particles observed via microscopy had completely failed PyC coatings. Some different DTF kernel and PyC morphologies were observed that have been attributed to the differences in irradiation temperature and/or burnup. At low and medium irradiation temperatures (roughly 850 to 1050°C), it appears irradiation-induced dimensional changes in the DTF PyC caused it to fracture and fold in on itself, and the kernel deformed to accommodate this PyC deformation. The extent of DTF kernel deformation to accommodate DTF PyC buckling tended to be greater for the medium-temperature fuel compared to the cold fuel. In the high-irradiation-temperature compact (1375°C), the DTF PyC layer appears to have completely reacted chemically with the kernel material. The only material surrounding the DTF particles in this hot compact resembles the compact matrix material. Observations of the TRISO-coated driver fuel particles and fuel compact graphitic matrix were also made. The TRISO particle kernels in the medium-temperature compact (1047°C), and many in the high-temperature compact (1375°C), showed morphologies consistent with what was seen in AGR-1 and AGR-2. In other TRISO particles from the high-temperature compact, a spatial gradient in the kernel appearance was evident. Here the cool side of the kernel (away from the center of the compact) had a much darker appearance (like that of the buffer and pyrocarbon). This is the first observation of this kind of kernel spatial gradient in AGR fuel. It is believed that the high irradiation temperature of that compact (an average of 1375°C) activates or accelerates an unidentified chemical reaction, and the temperature gradient within the fuel causes a sharp spatial gradient. The low-temperature/low-burnup compact also showed unique kernel morphologies where the TRISO-coated particles had clearly distinguishable oxide and carbide phases at the center of the kernel, an oxide rind surrounding this, and remnants of a carbide skin surrounding the oxide rind. These are features observed in the as-fabricated fuel that are no longer present in higher burnup fuel. Occasional gaps between the outer pyrolytic carbon (OPyC) and graphitic matrix material were observed in all compacts. Gaps were most often found in the small, matrix-filled spaces between adjacent particles. Finally, buffer morphologies in AGR-3/4 TRISO particles were like those observed in AGR-1 and AGR-2, and the buffer fracture frequencies in AGR-3/4 and AGR-2 TRISO particles were plotted versus fast neutron fluence and irradiation temperature. In AGR-3/4 the buffer fracture rate was highest (23%) for an irradiation temperature of 1047°C and a fast fluence of 5.18E25 n/m 2 . Increasing the irradiation temperature to 1375°C reduced the buffer fracture rate to 14%. Reducing the irradiation temperature to 872°C and the fast fluence by a factor of 3 reduced the buffer fracture rate to 7%. The temperature and fluence dependencies observed for AGR-3/4 buffer fracture were consistent with those observed in AGR-2. Lower fluences and/or higher temperatures significantly reduced the buffer fracture frequencies. Lower buffer fracture frequencies were also observed at lower temperatures as long as the fluence was also significantly reduced. This is believed to be due to lower fluence resulting in less irradiation-induced dimensional change (shrinkage) of the buffer, and a higher temperature promoting enhanced creep relaxation of stresses within the buffer, leading to less buffer fracture.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Drought legacies mediated by trait trade‐offs in soil microbiomes

Abstract Soil microbiomes play a key role in driving biogeochemical cycles of the Earth system. As drought frequency and intensity increase due to climate change, soil microbes and the processes they control will be impacted. Even after a drought ends, microbiomes and other systems take time to recover and may display a memory of previous climate conditions. Still, the mechanisms involved in these legacy effects remain unclear, making it difficult to predict climate and biogeochemical rates in the future. Here, we used a trait‐based microbiome model (DEMENTpy) to implement trade‐off‐mediated mechanisms that may lead to drought legacy effects on litter decomposition. Trade‐offs were assumed to follow the Y‐A‐S framework that defines three primary life‐history strategies of microorganisms: high growth Yield, resource Acquisition, and Stress tolerance. We represented cellular trade‐offs between osmolytes required for drought tolerance and investment in enzymes involved in litter decomposition. Simulations were run under varying levels of drought severity and dispersal. With high levels of dispersal, no legacy effects were predicted by DEMENTpy following drought. With limited dispersal, severe drought resulted in a persistent legacy of altered community‐level traits and reduced litter decomposition. Moderate drought resulted in a transient legacy that disappeared after two years, consistent with recent empirical observations in Southern California ecosystems. These results imply that greater movement along the trade‐off between enzyme investment and osmolyte production resulted in stronger legacy effects. More generally, factors that shift the position of a microbiome in YAS space may alter the legacy outcome following drought. Our trait‐based modeling study motivates additional empirical measurements to quantify YAS traits and trade‐offs that are needed to make accurate predictions of soil microbiome resilience and functioning. Also, our study illustrates an emerging approach for representing trait trade‐offs in microbiomes and vegetation that dictate ecosystem responses to drought and other environmental perturbations.

54 ENVIRONMENTAL SCIENCES↗

Adaptive Activation Functions Accelerate Convergence in Deep and Physics-informed Neural Networks

We employ adaptive activation functions for regression in deep and physics-informed neural networks (PINNs) to approximate smooth and discontinuous functions as well as solutions of linear and nonlinear partial differential equations. In particular, we solve the nonlinear Klein-Gordon equation, which has smooth solutions, the nonlinear Burgers equation, which can admit high gradient solutions, and the Helmholtz equation. We introduce a scalable hyper-parameter in the activation function, which can be optimized to achieve best performance of the network as it changes dynamically the topology of the loss function involved in the optimization process. The adaptive activation function has better learning capabilities than the traditional one (fixed activation) as it improves greatly the convergence rate, especially at early training, as well as the solution accuracy. To better understand the learning process, we plot the neural network solution in the frequency domain to examine how the network captures successively different frequency bands present in the solution. We consider both forward problems, where the approximate solutions are obtained, as well as inverse problems, where parameters involved in the governing equation are identified. Our simulation results show that the proposed method is a very simple and effective approach to increase the efficiency, robustness and accuracy of the neural network approximation of nonlinear functions as well as solutions of partial differential equations, especially for forward problems. We theoretically prove that in the proposed method, gradient descent algorithms are not attracted to suboptimal critical points or local minima.

machine leaning, Bad minima, Inverse problems, Phy↗