Explosive fragmentation of additively manufactured stainless steel
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We isolated 7 endospore forming bacteria from campus woodland and sequenced their genomes using Illumina NextSeq. We share the draft genome assemblies for strains Bacillus wiedmanii_SC129, Bacillus pseudomycoides_SC131, Bacillus pumilis_SC133, Peribacillus butanolivorans_SC135, Bacillus thuringiensis_SC136, Priestia megaterium_SC138, and Bacillus wiedmanii_SC141. Draft genomes are between 3645032-5969865 bp and 34.8-41.2 % GC.
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A solution-cathode glow discharge mass spectrometry (SCGD-MS) apparatus comprises a SCGD source and a mass spectrometer. The SCGD source may comprise conductive rods, a power source, and a capillary. A method for ionizing an analyte comprises flowing an electrically conductive liquid onto a conductive rod, applying an electric potential to a second conductive rod such that a plasma discharge forms between the first conductive rod and the electrically conductive liquid to produce ions, and separating the ions in a mass spectrometer. The analyte may be a polypeptide that may be contacted with trypsin. The analyte may be a solid, liquid, gas, chemical complex, or ion in solution. The method may comprise sequencing the polypeptide.
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Engineered protein electron carriers, microorganisms expressing the same, and methods detecting regulated electron flow are described.
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