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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 397 records · Page 22
All sourcesNASA NTRSDOE OSTI
DOE OSTI2025

Addendum: Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale

In response to a reader’s questions, here we provide additional information about the life cycle analysis (LCA) performed in this paper. The goal of the LCA was to understand the potential environmental benefits of our reported synthetic biology pathways for producing acetone and isopropanol by comparing greenhouse gas emissions to those from conventional, virgin fossil production routes. Below we expand on the rationale and sources underlying the methodological choices we made in the LCA, including our use of a cradle-to-gate system boundary, an avoided emissions credit and the descriptor “carbon-negative.” Finally, we also discuss differences in carbon accounting between an LCA framework and a carbon dioxide removal (CDR) framework.

metabolic engineering↗
DOE OSTI2017

Application of metabolic controls for the maximization of lipid production in semicontinuous fermentation

Significance This work establishes a semicontinuous process for efficient and complete upgrading of low-strength acetic acid into lipids. By implementing control strategies in response to the time-varying nature of cell metabolism, we achieved and sustained lipid yields close to the theoretical maximum while maintaining high productivities. The control algorithm was designed to respond to readily available real-time measurements and basic principles, which is ideal for industrial applications. Overall, our process scheme can directly take in dilute nutrient streams that are difficult and costly to purify and is able to biologically convert them into highly concentrated value-added products. This design significantly reduces the energy costs associated with processes using dilute feedstocks, which typically require initial concentration of the feed.

09 BIOMASS FUELS↗
DOE OSTI2014

Polyamine Transporters and Polyamines Increase Furfural Tolerance during Xylose Fermentation with Ethanologenic Escherichia coli Strain LY180

Expression of genes encoding polyamine transporters from plasmids and polyamine supplements increased furfural tolerance (growth and ethanol production) in ethanologenic Escherichia coli LY180 (in AM1 mineral salts medium containing xylose). Furthermore, this represents a new approach to increase furfural tolerance and may be useful for other organisms. Microarray comparisons of two furfural-resistant mutants (EMFR9 and EMFR35) provided initial evidence for the importance of polyamine transporters. Each mutant contained a single polyamine transporter gene that was upregulated over 100-fold (microarrays) compared to that in the parent LY180, as well as a mutation that silenced the expression of yqhD. Based on these genetic changes, furfural tolerance was substantially reconstructed in the parent, LY180.

59 BASIC BIOLOGICAL SCIENCES↗