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At least 397 records · Page 22

Avian Blood-Vessel Formation in Space

Based on previous studies, we hypothesized that the developmental anomalies observed in the past might be related to or caused by delayed or improper vascular development. The objective of our research is to test the hypothesis that exposure to microgravity during space flight cause delayed or improper vascular development during embryogenesis. The effects of microgravity on the time course and extent of avian blood-vessel formation are assessed using two models, one for angiogenesis and one for vasculogenesis. The methodological approach is dictated by the constraints of the tissue preservation method used in space. Thus, both in the chorioallantoic membrane (CAM) and in the adrenal, we will evaluate microscopically the vascular architecture and immunostain endothelial cells with specific antibodies (anti- vWF and QH1). The extent of ECM protein deposition will be assessed by immunohistochemistry and correlated with the degree of vascularization, using computer-based image analysis. Also, the cellular source for ECM proteins will be assessed by in situ hybridization.

Lelkes, Peter I.↗

Launch Conditions Might Affect the Formation of Blood Vessel in the Quail Chorioallantoic Membrane

AS 2 part of the first joint USA-Russian MIR/Shuttle program, fertilized quail eggs were flown on the MIR 18 mission. Post-flight examination indicated impaired survival of both the embryos in space and also of control embryos exposed to vibrational and g-forces simulating the conditions experienced during the launch of Progress 227. We hypothesized that excess mechanical forces and/or other conditions during the launch might cause abnormal development of the blood supply in the chorioallantoic membrane (CAM) leading to the impaired survival of the embryos. The CAM, a highly vascularized extraembryonic organ, provides for the oxygen exchange across the egg shell and is thus pivotal for proper embryonic development. To test our hypothesis, we compared angiogenesis In CAMS of eggs which were either exposed to the vibration and g-force profile simulating the conditions at launch of Progress 227 (synchronous controls), or kept under routine conditions in a laboratory Incubator (laboratory controls). At various time points during Incubation, the eggs were fixed in paraformaldehyde for subsequent dissection. At the time of dissection, the CAM was carefully lifted from the egg shell and examined as whole mounts by bright-field and fluorescent microscopy. The development or the vasculature (angiogenesis) was assessed from the density of blood vessels per viewing field and evaluated by computer aided image analysis. We observed a significant decrease In blood-vessel density in the synchronous controls versus "normal" laboratory controls beginning from day 10 of Incubation. The decrease in vascular density was restricted to the smallest vessels only, suggesting that conditions during the launch and/or during the subsequent Incubation of the eggs may affect the normal progress of angiogenesis in the CAM. Abnormal angiogenesis In the CAM might contribute to the impaired survival of the embryos observed in synchronous controls as well as in space.

Henry, M. K.↗

Autonomous Mobile Platform for Research in Cooperative Robotics

This paper describes the design and development of a platform for research in cooperative mobile robotics. The structure and mechanics of the vehicles are based on R/C cars. The vehicle is rendered mobile by a DC motor and servo motor. The perception of the robot's environment is achieved using IR sensors and a central vision system. A laptop computer processes images from a CCD camera located above the testing area to determine the position of objects in sight. This information is sent to each robot via RF modem. Each robot is operated by a Motorola 68HC11E micro-controller, and all actions of the robots are realized through the connections of IR sensors, modem, and motors. The intelligent behavior of each robot is based on a hierarchical fuzzy-rule based approach.

Daemi, Ali↗

Application of Overset Technology on SIMPLEX Turbopump Design

A viewgraph presentation shows computer generated images of the Simplex Turbopump. Machine schematics are shown with scaled details of the nozzle ring assay and the turbine gas flow path. Grid generation is used to show nozzle grids, blade contours, blade grids and simplex full configuration and geometry. A flow simulation shows mach contours in absolute and relative frames.

Vu, Bruce↗

Non-Intrusive, Laser-Based Imaging of Jet-A Fuel Injection and Combustion Species in High Pressure, Subsonic Flows

The emphasis of combustion research efforts at NASA Glenn Research Center (GRC) is on collaborating with industry to design and test gas-turbine combustors and subcomponents for both sub- and supersonic applications. These next-generation aircraft combustors are required to meet strict international environmental restrictions limiting emissions. To meet these goals, innovative combustor concepts require operation at temperatures and pressures far exceeding those of current designs. New and innovative diagnostic tools are necessary to characterize these flow streams since existing methods are inadequate. The combustion diagnostics team at GRC has implemented a suite of highly sensitive, nonintrusive optical imaging methods to diagnose the flowfields of these new engine concepts. By using optically accessible combustors and flame-tubes, imaging of fuel and intermediate combustion species via planar laser-induced fluorescence (PLIF) at realistic pressures are now possible. Direct imaging of the fuel injection process through both planar Mie scattering and PLIF methods is also performed. Additionally, a novel combination of planar fuel fluorescence imaging and computational analysis allows a 3-D examination of the flowfield, resulting in spatially and temporally resolved fuel/air volume distribution maps. These maps provide detailed insight into the fuel injection process at actual conditions, thereby greatly enhancing the evaluation of fuel injector performance and other combustion phenomena. Stable species such as CO2, O2, N2, H2O, and hydrocarbons are also investigated by a newly demonstrated 1-D, spontaneous Raman spectroscopic method. This visible wavelength Raman technique allows the acquisition of quantitative, stable species concentration measurements from the flow.

Locke, R. J.↗

Runway Texture and Grid Pattern Effects on Rate-of-Descent Perception

To date, perceptual errors occur in determining descent rate from a computer-generated image in flight simulation. Pilots tend to touch down twice as hard in simulation than in flight, and more training time is needed in simulation before reaching steady-state performance. Barnes suggested that recognition of range may be the culprit, and he cited that problems such as collimated objects, binocular vision, and poor resolution lead to poor estimation of the velocity vector. Brown's study essentially ruled out that the lack of binocular vision is the problem. Dorfel added specificity to the problem by showing that pilots underestimated range in simulated scenes by 50% when 800 ft from the runway threshold. Palmer and Petitt showed that pilots are able to distinguish between a 1.7 ft/sec and 2.9 ft/sec sink rate when passively observing sink rates in a night scene. Platform motion also plays a role, as previous research has shown that the addition of substantial platform motion improves pilot estimates of vertical velocity and results in simulated touchdown rates more closely resembling flight. This experiment examined how some specific variations in the visual scene properties affect a pilot's perception of sink rate. It extended another experiment that focused on the visual and motion cues necessary for helicopter autorotations. In that experiment, pilots performed steep approaches to a runway. The visual content of the runway and its surroundings varied in two ways: texture and rectangular grid spacing. Four textures, included a no-texture case, were evaluated. Three grid spacings, including a no-grid case, were evaluated. The results showed that pilot better controlled their vertical descent rates when good texture cues were present. No significant differences were found for the grid manipulation. Using those visual scenes a simple psychophysics, experiment was performed. The purpose was to determine if the variations in the visual scenes allowed pilots to better perceive vertical velocity. To determine that answer, pilots passively viewed a particular visual scene in which the vehicle was descending at two different rates. Pilots had to select which of the two rates they thought was the fastest rate. The difference between the two rates changed using a staircase method, depending on whether or not the pilot was correct, until a minimum threshold between the two descent rates was reached. This process was repeated for all of the visual scenes to decide whether or not the visual scenes did allow pilots to perceive vertical velocity better among them. All of the data have yet to be analyzed; however, neither the effects of grid nor texture revealed any statistically significant trends. On further examination of the staircase method employed, a possibility exists that the lack of an evident trend may be due to the exit criterion used during the study. As such, the experiment will be repeated with an improved exit criterion in February. Results of this study will be presented in the submitted paper.

Schroeder, J. A.↗

Non-Intrusive, Laser-Based Imaging of Jet-A Fuel Injection and Combustion Species in High Pressure, Subsonic Flows

The emphasis of combustion research efforts at NASA Glenn Research Center (GRC) is on collaborating with industry to design and test gas-turbine combustors and subcomponents for both sub- and supersonic applications. These next-generation aircraft combustors are required to meet strict international environmental restrictions limiting emissions. To meet these goals, innovative combustor concepts require operation at temperatures and pressures far exceeding those of cur-rent designs. New and innovative diagnostic tools are necessary to characterize these flow streams since existing methods are inadequate. The combustion diagnostics team at GRC has implemented a suite of highly sensitive, nonintrusive optical imaging methods to diagnose the flowfields of these new engine concepts. By using optically accessible combustors and flametubes, imaging of fuel and intermediate combustion species via planar laser-induced fluorescence (PLIF) at realistic pressures are now possible. Direct imaging of the fuel injection process through both planar Mie scattering and PLIF methods is also performed. Additionally, a novel combination of planar fuel fluorescence imaging and computational analysis allows a 3-D examination of the flowfield, resulting in spatially and temporally resolved fuel/air volume distribution maps. These maps provide detailed insight into the fuel injection process at actual conditions, thereby greatly enhancing the evaluation of fuel injector performance and other combustion phenomena. Stable species such as CO2, O2, N2O. and hydrocarbons are also investigated by a newly demonstrated 1-D, spontaneous Raman spectroscopic method. This visible wavelength Raman technique allows the acquisition of quantitative. stable species concentration measurements from the flow.

Locke, Randy J.↗

Determination of the Charon/Pluto Mass Ratio from Center-of-Light Astrometry

The Charon/Pluto mass ratio is a fundamental but poorly known parameter of the two-body system. Previous values for the mass ratio have ranged from 0.0837 plus or minus 0.0147 (Null et al., 1993, Astron. J. 105, 2319-2335) to 0.1566 plus or minus 0.0035 (Young et al., 1994, Icarus 108,186-199). We report here a new determination of the Charon/Pluto mass ratio, using five sets of groundbased images taken at four sites in support of Pluto occultation predictions. Unlike the Null et al. and Young et A determinations, where the centers of light for Pluto and Charon could be determined separately, this technique examines the motion of the center of light of the blended Pluto-Charon image. We compute the offsets of the observed center-of-light position of Pluto-Charon from the ephemeris position of the system and fit these offsets to a model of the Pluto-Charon system. The least-squares fits to the five data sets agree within their errors, and the weighted mean mass ratio is 0.117 plus or minus 0.006. The effects of errors in the Charon light fraction, semimajor axis, and ephemeris have been examined and are equal to only a small fraction of the formal error from the fit. This result is intermediate between those of Null et al., and Young et al. and matches a new value of 0.124 plus or minus 0.008 by Null and Owen (1996, Astron. J. 111, 1368-1381). The mass ratio and resulting individual masses and densities of Pluto and Charon are consistent with a collisional origin for the Pluto-Charon system.

Foust, Jeffrey A.↗

Marine Life Study

As a result of widespread ocean dumping and other pollution problems, marine scientists at Morgan State University are studying the populations of various marine organisms to determine the effects of pollution. They are also compiling data on the aging of marine organisms. There now exists a new method of determining the age of the surf clam. They are applying digital image processing to clam aging investigations. Computer creates digitized images of clam sections with annual rings. The image is enhanced -- manipulated to emphasize certain features in order to improve and amplify the information that can be extracted from the image. Also useful in other marine organisms that have growth bands making it easier to get an accurate count.

Source record↗

Digital Imaging

Digital Imaging is the computer processed numerical representation of physical images. Enhancement of images results in easier interpretation. Quantitative digital image analysis by Perceptive Scientific Instruments, locates objects within an image and measures them to extract quantitative information. Applications are CAT scanners, radiography, microscopy in medicine as well as various industrial and manufacturing uses. The PSICOM 327 performs all digital image analysis functions. It is based on Jet Propulsion Laboratory technology, is accurate and cost efficient.

Source record↗

X-ray single Photon Spectrometers

The research at Yale on superconducting tunnel junctions (STJs) focussed on x-ray detectors, using a 5.9 keV x-ray source. All the Yale tests were carried out at 0.21 K, in a two-stage helium 3 dewar. Careful attention was given to grounding and shielding. All current waveforms were digitially recorded and analyzed subsequently on computer. The imaging device geometry was studied. This allows imaging along the length of the tantalum (Ta) absorber, which is 600 nm thick to provide good x-ray absorption. The quasiparticles produced by single photon absorption rapidly cool to the Ta gap, E(sub g_Ta) = 1.4 meV, and then diffuse. They are trapped in the A1 traps at the two ends (E(sub g_A1) = 0.4 meV), to give two charges that tunnel out of the A1 traps, Q(sub 1) and Q(sub 2). The total charge is Q = Q(sub 1) + Q(sub 2). This lateral trapping is what allows imaging.

Prober, Daniel↗

A CAD Approach to Integrating NDE With Finite Element

Nondestructive evaluation (NDE) is one of several technologies applied at NASA Glenn Research Center to determine atypical deformities, cracks, and other anomalies experienced by structural components. NDE consists of applying high-quality imaging techniques (such as x-ray imaging and computed tomography (CT)) to discover hidden manufactured flaws in a structure. Efforts are in progress to integrate NDE with the finite element (FE) computational method to perform detailed structural analysis of a given component. This report presents the core outlines for an in-house technical procedure that incorporates this combined NDE-FE interrelation. An example is presented to demonstrate the applicability of this analytical procedure. FE analysis of a test specimen is performed, and the resulting von Mises stresses and the stress concentrations near the anomalies are observed, which indicates the fidelity of the procedure. Additional information elaborating on the steps needed to perform such an analysis is clearly presented in the form of mini step-by-step guidelines.

Abdul-Aziz, Ali↗

Contributions of the NASA Langley Research Center to the DARPA/AFRL/NASA/ Northrop Grumman Smart Wing Program

An overview of the contributions of the NASA Langley Research Center (LaRC) to the DARPA/AFRL/NASA/ Northrop Grumman Corporation (NGC) Smart Wing program is presented. The overall objective of the Smart Wing program was to develop smart** technologies and demonstrate near-flight-scale actuation systems to improve the aerodynamic performance of military aircraft. NASA LaRC s roles were to provide technical guidance, wind-tunnel testing time and support, and Computational Fluid Dynamics (CFD) analyses. The program was divided into two phases, with each phase having two wind-tunnel entries in the Langley Transonic Dynamics Tunnel (TDT). This paper focuses on the fourth and final wind-tunnel test: Phase 2, Test 2. During this test, a model based on the NGC Unmanned Combat Air Vehicle (UCAV) concept was tested at Mach numbers up to 0.8 and dynamic pressures up to 150 psf to determine the aerodynamic performance benefits that could be achieved using hingeless, smoothly-contoured control surfaces actuated with smart materials technologies. The UCAV-based model was a 30% geometric scale, full-span, sting-mounted model with the smart control surfaces on the starboard wing and conventional, hinged control surfaces on the port wing. Two LaRC-developed instrumentation systems were used during the test to externally measure the shapes of the smart control surface and quantify the effects of aerodynamic loading on the deflections: Videogrammetric Model Deformation (VMD) and Projection Moire Interferometry (PMI). VMD is an optical technique that uses single-camera photogrammetric tracking of discrete targets to determine deflections at specific points. PMI provides spatially continuous measurements of model deformation by computationally analyzing images of a grid projected onto the model surface. Both the VMD and PMI measurements served well to validate the use of on-board (internal) rotary potentiometers to measure the smart control surface deflection angles. Prior to the final entry, NASA LaRC also performed three-dimensional unstructured Navier Stokes CFD analyses in an attempt to predict the potential aerodynamic impact of the smart control surface on overall model forces and moments. Eight different control surface shapes were selected for study at Mach = 0.6, Reynolds number = 3.25 x 10(exp 6), and + 2 deg., 3 deg., 8 deg., and 10 deg.model angles-of-attack. For the baseline, undeflected control surface geometry, the CFD predictions and wind-tunnel results matched well. The agreement was not as good for the more complex aero-loaded control surface shapes, though, because of the inability to accurately predict those shapes. Despite these results, the NASA CFD study served as an important step in studying advanced control effectors.

Florance, Jennifer P.↗

Orientation processing mechanisms revealed by the plaid tilt illusion

The tilt after-effect (TAE) and tilt illusion (TI) have revealed a great deal about the nature of orientation coding of 1-dimensional (1D) lines and gratings. Comparatively little research however has addressed the mechanisms responsible for encoding the orientation of 2-dimensional (2D) plaid stimuli. A multi-stage model of edge detection has recently been proposed [Georgeson, M. A. (1998) Image & Vision Computing, 16(6-7), 389-405] to account for the perceived structure of a plaid stimulus that incorporates extraction of the zero-crossings (ZCs) of the plaid. Data is presented showing that the ZCs of a plaid inducing stimulus can interact with vertical grating test stimulus to induce a standard tilt illusion. However, by considering the second-order structure of a plaid rather than ZCs, it was shown that the perceived orientation of the vertical test grating results from the combination of orientation illusions due to the first- and second-order components of an inducing plaid. The data suggest that the mechanisms encoding the orientation of second-order contours are similar to, and interact directly with, those that encode first-order contours.

Orientation/physiology↗

Plastid sedimentation kinetics in roots of wild-type and starch-deficient mutants of Arabidopsis

Sedimentation and movement of plastids in columella cells of the root cap were measured in seedlings of wild-type, a reduced starch mutant, and a starchless mutant of Arabidopsis. To assay for sedimentation, we used both linear measurements and the change of angle from the cell center as indices in vertical and reoriented plants with the aid of computer-assisted image analysis. Seedlings were fixed at short periods after reorientation, and plastid sedimentation correlated with starch content in the three strains of Arabidopsis. Amyloplasts of wild-type seedlings showed the greatest sedimentation, whereas plastids of the starchless mutant showed no significant sedimentation in the vertically grown and reoriented seedlings. Because previous research has shown that a full complement of starch is needed for full gravitropic sensitivity, this study correlates increased sensitivity with plastid sedimentation. However, although plastid sedimentation contributed to gravisensitivity, it was not required, because the gravitropic starchless mutant had plastids that did not sediment. This is the first study, to our knowledge, to measure plastid sedimentation in Arabidopsis roots after reorientation of seedlings. Taken together, the results of this study are consistent with the classic plastid-based and protoplast-based models of graviperception and suggest that multiple systems of perception exist in plant cells.

NASA Discipline Plant Biology↗

Influence of spaceflight on succinate dehydrogenase activity and soma size of rat ventral horn neurons

Succinate dehydrogenase (SDH) activities and soma cross-sectional areas (CSA) of neurons in the dorsolateral region of the ventral horn at the L5 segmental level of the spinal cord in the rat were determined after 14 days of spaceflight and after 9 days of recovery on earth. The results were compared to those in age-matched ground-based control rats. Spinal cords were quick-frozen, and the SDH activity and CSA of a sample of neurons with a visible nucleus were determined using a digitizer and a computer-assisted image analysis system. An inverse relationship between CSA and SDH activity of neurons was observed in all groups of rats. No change in mean CSA or mean SDH activity or in the size distribution of neurons was observed following spaceflight or recovery. However, there was a selective decrease in the SDH activity of neurons with soma CSA between 500 and 800 microns2 in the flight rats, and this effect persisted for at least 9 days following return to 1 g. It remains to be determined whether the selected population of motoneurons or the specific motor pools affected by spaceflight may be restricted to specific muscles.

short duration↗

Visual Cueing Effects Investigation for a Hovering Task

In simulators, as in actual aircraft, pilots conduct their missions according to perceived vehicle performance and spatial orientation from out-the-window (OTW) information. Ground-based flight simulations rely upon computer-generated OTW visual scenes. The realism and fidelity of a specific OTW simulation therefore has a significant impact on mission effectiveness. This study investigates effects of three visual parameters of the computer-generated image system (field-of-view, collimation, and resolution) on a disturbance-rejection hovering task. Pilots' performance and workload were studied under the following conditions: collimated optics vs. noncollimated optics, wide field-of-view (FOV) vs. narrow FOV, and higher resolution vs. lower resolution. Subjects were instructed to maintain a station-keeping position during disturbances while piloting an uncoupled four degrees-of-freedom (DOF) helicopter model, which had a satisfactory rate-command system in pitch and roll axes and ideal heading and altitude hold. Display collimation and FOV were found to have significant effects on pilots performance and subjective their subjective perception of visual cueing quality.

Chung, William W. Y.↗

Geospatial Analysis and Remote Sensing from Airplanes and Satellites for Cultural Resources Management

Cultural resource management consists of research to identify, evaluate, document and assess cultural resources, planning to assist in decision-making, and stewardship to implement the preservation, protection and interpretation of these decisions and plans. One technique that may be useful in cultural resource management archaeology is remote sensing. It is the acquisition of data and derivative information about objects or materials (targets) located on the Earth's surface or in its atmosphere by using sensor mounted on platforms located at a distance from the targets to make measurements on interactions between the targets and electromagnetic radiation. Included in this definition are systems that acquire imagery by photographic methods and digital multispectral sensors. Data collected by digital multispectral sensors on aircraft and satellite platforms play a prominent role in many earth science applications, including land cover mapping, geology, soil science, agriculture, forestry, water resource management, urban and regional planning, and environmental assessments. Inherent in the analysis of remotely sensed data is the use of computer-based image processing techniques. Geographical information systems (GIS), designed for collecting, managing, and analyzing spatial information, are also useful in the analysis of remotely sensed data. A GIS can be used to integrate diverse types of spatially referenced digital data, including remotely sensed and map data. In archaeology, these tools have been used in various ways to aid in cultural resource projects. For example, they have been used to predict the presence of archaeological resources using modern environmental indicators. Remote sensing techniques have also been used to directly detect the presence of unknown sites based on the impact of past occupation on the Earth's surface. Additionally, remote sensing has been used as a mapping tool aimed at delineating the boundaries of a site or mapping previously unknown features. All of these applications are pertinent to the goals of site discovery and assessment in cultural resource management.

Giardino, Marco J.↗