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At least 379 records · Page 21

The potato virus X TGBp2 protein association with the endoplasmic reticulum plays a role in but is not sufficient for viral cell-to-cell movement

Potato virus X (PVX) TGBp1, TGBp2, TGBp3, and coat protein are required for virus cell-to-cell movement. Plasmids expressing GFP fused to TGBp2 were bombarded to leaf epidermal cells and GFP:TGBp2 moved cell to cell in Nicotiana benthamiana leaves but not in Nicotiana tabacum leaves. GFP:TGBp2 movement was observed in TGBp1-transgenic N. tabacum, indicating that TGBp2 requires TGBp1 to promote its movement in N. tabacum. In this study, GFP:TGBp2 was detected in a polygonal pattern that resembles the endoplasmic reticulum (ER) network. Amino acid sequence analysis revealed TGBp2 has two putative transmembrane domains. Two mutations separately introduced into the coding sequences encompassing the putative transmembrane domains within the GFP:TGBp2 plasmids and PVX genome, disrupted membrane binding of GFP:TGBp2, inhibited GFP:TGBp2 movement in N. benthamiana and TGBp1-expressing N. tabacum, and inhibited PVX movement. A third mutation, lying outside the transmembrane domains, had no effect on GFP:TGBp2 ER association or movement in N. benthamiana but inhibited GFP:TGBp2 movement in TGBp1-expressing N. tabacum and PVX movement in either Nicotiana species. Thus, ER association of TGBp2 may be required but not be sufficient for virus movement. TGBp2 likely provides an activity for PVX movement beyond ER association.

NASA Discipline Plant Biology↗

Computational Analysis of a Chevron Nozzle Uniquely Tailored for Propulsion Airframe Aeroacoustics

A computational flow field and predicted jet noise source analysis is presented for asymmetrical fan chevrons on a modern separate flow nozzle at take off conditions. The propulsion airframe aeroacoustic asymmetric fan nozzle is designed with an azimuthally varying chevron pattern with longer chevrons close to the pylon. A baseline round nozzle without chevrons and a reference nozzle with azimuthally uniform chevrons are also studied. The intent of the asymmetric fan chevron nozzle was to improve the noise reduction potential by creating a favorable propulsion airframe aeroacoustic interaction effect between the pylon and chevron nozzle. This favorable interaction and improved noise reduction was observed in model scale tests and flight test data and has been reported in other studies. The goal of this study was to identify the fundamental flow and noise source mechanisms. The flow simulation uses the asymptotically steady, compressible Reynolds averaged Navier-Stokes equations on a structured grid. Flow computations are performed using the parallel, multi-block, structured grid code PAB3D. Local noise sources were mapped and integrated computationally using the Jet3D code based upon the Lighthill Acoustic Analogy with anisotropic Reynolds stress modeling. In this study, trends of noise reduction were correctly predicted. Jet3D was also utilized to produce noise source maps that were then correlated to local flow features. The flow studies show that asymmetry of the longer fan chevrons near the pylon work to reduce the strength of the secondary flow induced by the pylon itself, such that the asymmetric merging of the fan and core shear layers is significantly delayed. The effect is to reduce the peak turbulence kinetic energy and shift it downstream, reducing overall noise production. This combined flow and noise prediction approach has yielded considerable understanding of the physics of a fan chevron nozzle designed to include propulsion airframe aeroacoustic interaction effects.

Massey, Steven J.↗

Rapid flow fractionation of particles combining liquid and particulate dielectrophoresis

Rapid, size-based, deposition of particles from liquid suspension is accomplished using a nonuniform electric field created by coplanar microelectrode strips patterned on an insulating substrate. The scheme uses the dielectrophoretic force both to distribute aqueous liquid containing particles and, simultaneously, to separate the particles. Size-based separation is found within nanoliter droplets formed along the structure after voltage removal. Bioparticles or macromolecules of similar size can also be separated based on subtle differences in dielectric property, by controlling the frequency of the AC current supplied to the electrodes.

King, Michael R.↗

North American Extreme Temperature Events and Related Large Scale Meteorological Patterns: A Review of Statistical Methods, Dynamics, Modeling, and Trends

The objective of this paper is to review statistical methods, dynamics, modeling efforts, and trends related to temperature extremes, with a focus upon extreme events of short duration that affect parts of North America. These events are associated with large scale meteorological patterns (LSMPs). The statistics, dynamics, and modeling sections of this paper are written to be autonomous and so can be read separately. Methods to define extreme events statistics and to identify and connect LSMPs to extreme temperature events are presented. Recent advances in statistical techniques connect LSMPs to extreme temperatures through appropriately defined covariates that supplement more straightforward analyses. Various LSMPs, ranging from synoptic to planetary scale structures, are associated with extreme temperature events. Current knowledge about the synoptics and the dynamical mechanisms leading to the associated LSMPs is incomplete. Systematic studies of: the physics of LSMP life cycles, comprehensive model assessment of LSMP-extreme temperature event linkages, and LSMP properties are needed. Generally, climate models capture observed properties of heat waves and cold air outbreaks with some fidelity. However they overestimate warm wave frequency and underestimate cold air outbreak frequency, and underestimate the collective influence of low-frequency modes on temperature extremes. Modeling studies have identified the impact of large-scale circulation anomalies and landatmosphere interactions on changes in extreme temperatures. However, few studies have examined changes in LSMPs to more specifically understand the role of LSMPs on past and future extreme temperature changes. Even though LSMPs are resolvable by global and regional climate models, they are not necessarily well simulated. The paper concludes with unresolved issues and research questions.

Cold Spells↗

Analysis of Ares Crew Launch Vehicle Transonic Alternating Flow Phenomenon

A transonic wind tunnel test of the Ares I-X Rigid Buffet Model (RBM) identified a Mach number regime where unusually large buffet loads are present. A subsequent investigation identified the cause of these loads to be an alternating flow phenomenon at the Crew Module-Service Module junction. The conical design of the Ares I-X Crew Module and the cylindrical design of the Service Module exposes the vehicle to unsteady pressure loads due to the sudden transition between a subsonic separated and a supersonic attached flow about the cone-cylinder junction as the local flow randomly fluctuates back and forth between the two flow states. These fluctuations produce a square-wave like pattern in the pressure time histories resulting in large amplitude, impulsive buffet loads. Subsequent testing of the Ares I RBM found much lower buffet loads since the evolved Ares I design includes an ogive fairing that covers the Crew Module-Service Module junction, thereby making the vehicle less susceptible to the onset of alternating flow. An analysis of the alternating flow separation and attachment phenomenon indicates that the phenomenon is most severe at low angles of attack and exacerbated by the presence of vehicle protuberances. A launch vehicle may experience either a single or, at most, a few impulsive loads since it is constantly accelerating during ascent rather than dwelling at constant flow conditions in a wind tunnel. A comparison of a windtunnel- test-data-derived impulsive load to flight-test-data-derived load indicates a significant over-prediction in the magnitude and duration of the buffet load. I. Introduction One

Sekula, Martin K.↗

Texture measurements for the automatic classification of imagery

The stated purpose is to demonstrate the applicability of texture measurements for making distinctions between classes of imagery. Multispectral images obtained from aircraft and satellites have been successfully delineated into land use classes on the basis of density in the different spectral bands. However, spatial patterns can add additional information to improve classification accuracy. A comparison is made between the results obtained using five texture algorithms for separating land use classes using ERTS imagery. The transforms evaluated are the Karhunen-Loeve, the fast Fourier, the Walsh-Hadamard, the Slant, and a digital matched filter.

Kirvida, L.↗

Renal electrolyte circadian rhythms - Independence from feeding and activity patterns

Experiments were conducted on six unanesthetized chair-acclimatized adult male squirrel monkeys (Saimiri sciureus) weighing 600-900 g to determine whether internal synchronization is the result of simple passive dependence of renal excretory rhythms on endogenous rhythms of those variable that influence electrolyte excretion such as dietary intake and muscular activity. Independence of the urinary rhythms from diurnal variations in feeding, drinking, and activity was secured by depriving the animals of food, water, and training them to perform a two-hourly schedule of feeding, drinking, and activity throughout day and night. Results indicate that the internal synchronization which is normally observed between the behavioral and urinary rhythms cannot be explained by any direct dependence of renal function on behavioral patterns. The most probable mechanism for circadian internal synchronization is that the various behavioral and renal rhythms are controlled by potentially independent separate oscillators which are normally kept in synchrony with one another.

Moore-Ede, M. C.↗

The results of initial analysis of OSTA-1/Ocean Color Experiment (OCE) imagery

Ocean view images from the Ocean Color Experiment (OCE) were produced at three widely separated locations on the Earth. Digital computer enhancement and band ratioing techniques were applied to radiometrically corrected OCE spectral data to emphasize patterns of chlorophyll distribution and, in one shallow, clear water case, bottom topography. The chlorophyll pattern in the Yellow Sea between China and Korea was evident in a scene produced from Shuttle Orbit 24. The effects of the discharge from the Yangtze and other rivers were also observed. Two scenes from orbits 30 and 32 revealed the movement of patches of plankton in the Gulf of Cadiz. Geometrical corrections to these images permitted the existing ocean current velocities in the vicinity to be deduced. The variability in water depth over the Grand Bahama Bank was estimated by using the blue-green OCE channel. The very clear water conditions in the area caused bottom reflected sunlight to produce a sensor signal which was related inversely to the depth of the water.

Kim, H. H.↗

Supersonic separated flow past a cylindrical obstacle on a flat plate

An experimental investigation of three-dimensional boundary-layer separation on a flat plate ahead of a circular cylinder at Mach 2.36 was made. Emphasis was given to the laminar flow regime and to the flow region upstream of the cylinder. The heights and diameters of the cylinders used in the study were larger than the undisturbed boundary-layer thickness at the cylinder location. Data were obtained by oil flow visualization, Schlieren observations, static pressure measurements, and laser anemometry. Oil flow visualization revealed three separation lines on the flat plate ahead of the cylinder. A postulated flowfield structure, which was suggested by this skin-friction pattern, could not be confirmed by the velocity measurements. Velocity measurements indicated an unsteady flow structure.

Ozcan, O.↗

Chaotic nature of the spin-glass phase

The microscopic structure of the ordered phase of spin glasses is investigated theoretically in the framework of the T = 0 fixed-point model (McMillan, 1984; Fisher and Huse, 1986; and Bray and Moore, 1986). The sensitivity of the ground state to changes in the interaction strengths at T = 0 is explored, and it is found that for sufficiently large length scales the ground state is unstable against arbitrarily weak perturbations to the bonds. Explicit results are derived for d = 1, and the implications for d = 2 and d = 3 are considered in detail. It is concluded that there is no hidden order pattern for spin glasses at all T less than T(C), the ordered-phase spin correlations being chaotic functions of spin separation at fixed temperature or of temperature (for a given pair of spins) at scale lengths L greater than (T delta T) exp -1/zeta, where zeta = d(s)/2 - y, d(s) is the interfacial fractal dimension, and -y is the thermal eigenvalue at T = 0.

Bray, A. J.↗

Evidence for divergent plate-boundary characteristics and crustal spreading on Venus

Detailed examination of the topography and morphology of western Aphrodite Terra reveals numerous features that are similar to terrestrial divergent plate-boundary characteristics. Individual domains between fracture-zone-like discontinuities contain a variety of bilaterally symmetrical topographic elements that suggest that topographic features have been created at rise crests, rifted and separated, and moved laterally to their present symmetrical positions. The topographic and morphologic similarities, together with strikingly similar mirror-image map patterns on both sides of the rise axis, suggest that western Aphrodite Terra shares the characteristics of oceanic divergent plate boundaries, and is the site of crustal spreading on Venus. Topographic profiles are consistent with spreading rates of the order of several centimeters per year.

Head, James W., III↗

The undetected error probability for Reed-Solomon codes

McEliece and Swanson (1986) offered an upper bound on P(E)u, the decoder error probability given u symbol errors occur. In the present study, by using a combinatoric technique such as the principle of inclusion and exclusion, an exact formula for P(E)u is derived. The P(E)u of a maximum distance separable code is observed to approach Q rapidly as u gets large, where Q is the probability that a completely random error pattern will cause decoder error. An upper bound for the expansion P(E)u/Q - 1 is derived, and is shown to decrease nearly exponentially as u increases. This proves analytically that P(E)u indeed approaches Q as u becomes large, and that some laws of large number come into play.

Cheung, Kar-Ming↗

Effect of abrupt pressure gradients on the structure of turbulent boundary layers

The structure of a turbulent boundary layer due to abruptly applied strong favorable and adverse pressure gradients, leading to relaminarization and separation, respectively, is considered. Digital image enhancement techniques have been applied to laser light sheet smoke flow visualization pictures. A 'local range modification' technique has been used to extract structural details otherwise lying ill-defined in the fully turbulent core. The cross-stream sections immediately prior to separation, and particularly those shortly after the application of the favorable pressure gradient, are vividly organized; there exists a criss-cross pattern in the spanwise plane with a mean orientation at about +/- 45 deg to the vertical.

Bandyopadhyay, Promode R.↗

Evolution of vector magnetic fields and the August 27 1990 X-3 flare

Vector magnetic fields in an active region of the sun are studied by means of continuous observations of magnetic-field evolution emphasizing magnetic shear build-up. The vector magnetograms are shown to measure magnetic fields correctly based on concurrent observations and a comparison of the transverse field with the H alpha fibril structure. The morphology and velocity pattern are examined, and these data and the shear build-up suggest that the active region's two major footprints are separated by a region with flows, new flux emergence, and several neutral lines. The magnetic shear appears to be caused by the collision and shear motion of two poles of opposite polarities. The transverse field is shown to turn from potential to sheared during the process of flux cancellation, and this effect can be incorporated into existing models of magnetic flux cancellation.

Wang, Haimin↗

Development of generalized 3-D braiding machines for composite preforms

The development of prototype braiding machines for the production of generalized braid patterns is described. Mechanical operating principles and control strategies are presented for two prototype machines which have been fabricated and evaluated. Both machines represent advances over current fabrication techniques for composite materials by enabling nearly ideal control of fiber orientations within preform structures. They permit optimum design of parts that might be subjected to complex loads or that have complex forms. Further, they overcome both the lack of general control of produced fiber architectures and the complexity of other weaving processes that have been proposed for the same purpose. One prototype, the Farley braider, consists of an array of turntables that can be made to oscillate in 90 degree steps. Yarn ends are transported about the surface formed by the turntables by motorized tractors which are controlled through an optical link with the turntables and powered through electrical contact with the turntables. The necessary relative motions are produced by a series of linear tractor moves combined with a series of turntable rotations. As the tractors move about, they weave the yarn ends into the desired pattern. The second device, the shuttle plate braider, consists of a braiding surface formed by an array of stationary square sections, each separated from its neighbors by a gap. A plate beneath this surface is caused to reciprocate in two perpendicular directions, first in one direction and then in the other. This movement is made possibly by openings in the plate that clear short columns supporting the surface segments. Yarn ends are moved about the surface and interwoven by shuttles which engage the reciprocating plate as needed to yield the desired movements. Power and control signals are transmitted to the shuttles through electrical contact with the braiding surface. The shuttle plate is a passively driven prime mover that supplies the power to move all shuttles and the shuttles are simple devices that employ only a solenoid to engage the shuttle plate on command. Each shuttle is assigned a unique identity and is controlled independently. When compared to each other, the Farley braider is felt to have the advantage of speed and the shuttle plate braider, the advantage of simplicity.

Huey, Cecil O., Jr.↗

A two-dimensional particle-in-cell model of a dusty plasma

Dusty plasmas are present in comets, in the ring systems of the outer planets, and in the interstellar medium. A two-dimensional particle-in-cell (PIC) model of a dusty plasma is presented in this paper. The PIC code is best suited for modeling the plasma-dust interaction for large grains, with diameters of the order of a centimeter. We have modeled the charging process for an individual dust grain and the associated potential pattern in the surrounding plasma. We have also considered the case of a large number of grains in a plasma, with intergrain separations of the order of the Debye length, and have shown that the plasma becomes depleted and the charge on a dust grain is reduced, as other workers in this field have predicted (cf. C. K. Goertz, 1989). We examine the electron and ion distribution functions in the vicinity of a charged grain and demonstrate that the ions near a grain have clearly been accelerated by the electrostatic potential.

Young, B.↗

Porting Gravitational Wave Signal Extraction to Parallel Virtual Machine (PVM)

Laser Interferometer Space Antenna (LISA) is a planned NASA-ESA mission to be launched around 2012. The Gravitational Wave detection is fundamentally the determination of frequency, source parameters, and waveform amplitude derived in a specific order from the interferometric time-series of the rotating LISA spacecrafts. The LISA Science Team has developed a Mock LISA Data Challenge intended to promote the testing of complicated nested search algorithms to detect the 100-1 millihertz frequency signals at amplitudes of 10E-21. However, it has become clear that, sequential search of the parameters is very time consuming and ultra-sensitive; hence, a new strategy has been developed. Parallelization of existing sequential search algorithms of Gravitational Wave signal identification consists of decomposing sequential search loops, beginning with outermost loops and working inward. In this process, the main challenge is to detect interdependencies among loops and partitioning the loops so as to preserve concurrency. Existing parallel programs are based upon either shared memory or distributed memory paradigms. In PVM, master and node programs are used to execute parallelization and process spawning. The PVM can handle process management and process addressing schemes using a virtual machine configuration. The task scheduling and the messaging and signaling can be implemented efficiently for the LISA Gravitational Wave search process using a master and 6 nodes. This approach is accomplished using a server that is available at NASA Ames Research Center, and has been dedicated to the LISA Data Challenge Competition. Historically, gravitational wave and source identification parameters have taken around 7 days in this dedicated single thread Linux based server. Using PVM approach, the parameter extraction problem can be reduced to within a day. The low frequency computation and a proxy signal-to-noise ratio are calculated in separate nodes that are controlled by the master using message and vector of data passing. The message passing among nodes follows a pattern of synchronous and asynchronous send-and-receive protocols. The communication model and the message buffers are allocated dynamically to address rapid search of gravitational wave source information in the Mock LISA data sets.

Thirumalainambi, Rajkumar↗

GEM-CEDAR Challenge: Comparing Ionospheric Models with Poynting Flux from DMSP Observations

As part to the GEM-CEDAR challenge we are extending the model-data comparisons to electrodynamic in-situ measurements in low-Earth orbit. We use DMSP observations of electric and magnetic fields to compute Poynting Flux values along the satellite track in high latitudes including the auroral zones and the polar cap. Models of the ionosphere that include electrodynamic parameters have been run for five events selected for the GEM-CEDAR modeling challenge for which DMSP data are available for comparison. Combined with a magnetic field model we use the modeled electric fields to compute Poynting Flux and Joule Dissipation values from outputs of CTIPe, TIE-GCM, the ionospheric electrodynamics solvers of the SWMF, LFM and OpenGGCM magnetosphere-ionosphere coupled models, and the Weimer electric field model. The online metrics analysis tool at the Community Coordinated Modeling Center (CCMC) has been updated to handle the analysis of separate short segments of available data (high-latitude sections of the satellite orbit) with model outputs to analyze how well auroral patterns are being reproduced by the models. We present initial results from the new analysis tool in terms of model yields (ratio of the difference between maximum and minimum values of model results to the observation), timing/location errors of local maxima in the inbound and outbound auroral crossings as well as cross-correlations for individual passes. We collect the information for many DMSP passes and present an analysis for model performance during quiet and geomagnetically disturbed time periods using half-orbit integrated values as well.

Rastaetter, Lutz↗