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At least 379 records · Page 21
All sourcesNASA NTRSDOE OSTI
NASA NTRS2016

Skeletal Micro-RNA Responses to Simulated Weightlessness

Astronauts lose bone structure during long-duration spaceflight. These changes are due, in part, to insufficient bone formation by the osteoblast cells. Little is known about the role that small (approximately 22 nucleotides), non-coding micro-RNAs (miRNAs) play in the osteoblast response to microgravity. We hypothesize that osteoblast-lineage cells alter their miRNA status during microgravity exposure, contributing to impaired bone formation during weightlessness. To simulate weightlessness, female mice (C57BL/6, Charles River, 10 weeks of age, n = 7) were hindlimb unloaded up to 12 days. Age-matched and normally ambulating mice served as controls (n=7). To assess the expression of miRNAs in skeletal tissue, the tibia was collected ex vivo and cleaned of soft-tissue and marrow. Total RNA was collected from tibial bone and relative abundance was measured for miRNAs of interest using quantitative real time PCR array looking at 372 unique and well-characterized mature miRNAs using the delta-delta Ct method. Transcripts of interest were normalized to an average of 6 reference RNAs. Preliminary results show that hindlimb unloading decreased the expression of 14 miRNAs to less than 0.5 times that of the control levels and increased the expression of 5 miRNAs relative to the control mice between 1.2-1.5-fold (p less than 0.05, respectively). Using the miRSystem we assessed overlapping target genes predicted to be regulated by multiple members of the 19 differentially expressed miRNAs as well as in silico predicted targets of our individual miRNAs. Our miRsystem results indicated that a number of our differentially expressed miRNAs were regulators of genes related to the Wnt-Beta Catenin pathway-a known regulator of bone health-and, interestingly, the estrogen-mediated cell-cycle regulation pathway, which may indicate that simulated weightlessness modulated systemic hormonal levels or hormonal transduction that additionally contributed to bone loss. We plan to follow up these findings by measuring gene expression of miRNA-regulated genes within these two pathways with the aim of furthering our understanding of the function of miRNAs in the skeletal response to spaceflight.

musculoskeletal system↗
NASA NTRS1993

Semi-discrete Galerkin solution of the compressible boundary-layer equations with viscous-inviscid interaction

A semi-discrete Galerkin (SDG) method is under development to model attached, turbulent, and compressible boundary layers for transonic airfoil analysis problems. For the boundary-layer formulation the method models the spatial variable normal to the surface with linear finite elements and the time-like variable with finite differences. A Dorodnitsyn transformed system of equations is used to bound the infinite spatial domain thereby providing high resolution near the wall and permitting the use of a uniform finite element grid which automatically follows boundary-layer growth. The second-order accurate Crank-Nicholson scheme is applied along with a linearization method to take advantage of the parabolic nature of the boundary-layer equations and generate a non-iterative marching routine. The SDG code can be applied to any smoothly-connected airfoil shape without modification and can be coupled to any inviscid flow solver. In this analysis, a direct viscous-inviscid interaction is accomplished between the Euler and boundary-layer codes through the application of a transpiration velocity boundary condition. Results are presented for compressible turbulent flow past RAE 2822 and NACA 0012 airfoils at various freestream Mach numbers, Reynolds numbers, and angles of attack.

Day, Brad A.↗
NASA NTRS1984

Simultaneous ocean cross-section and rainfall measurements from space with a nadir-pointing radar

A method to determine simultaneously the rainfall rate and the normalized backscattering cross section of the surface was evaluated. The method is based on the mirror reflected power, p sub m which corresponds to the portion of the incident power scattered from the surface to the precipitation, intercepted by the precipitation, and again returned to the surface where it is scattered a final time back to the antenna. Two approximations are obtained for P sub m depending on whether the field of view at the surface is either much greater or much less than the height of the reflection layer. Since the dependence of P sub m on the backscattering cross section of the surface differs in the two cases, two algorithms are given by which the path averaged rain rate and normalized cross section are deduced. The detectability of P sub m, the relative strength of other contributions to the return power arriving simultaneous with P sub m, and the validity of the approximations used in deriving P sub m are discussed.

Meneghini, R.↗
NASA NTRS1935

A preliminary determination of normal accelerations on racing airplanes

Rules and methods for insuring safe structural strength of racing airplanes used in the major air meets in this country have recently been considered. Acceleration records made in racing airplanes during actual air races were therefore considered desirable, and the NACA undertook the measurement of acceleration of loads on airplanes during all conditions of flight. Accelerations were measured on four airplanes at the Miami All-American Races in January 1934 and January 1935. The airplanes were representative of the fastest limited and unlimited displacement racing airplanes in current use in this country. Records during two races, or flights, on the race course were obtained with each airplane. The maximum normal acceleration recorded was 6.2g and the minimum was -1.2g.

Scudder, N F↗
NASA NTRS2016

Regulation of Bone Formation During Disuse by miRNA

Astronauts lose bone structure during long-duration spaceflight. These changes are due, in part, to insufficient bone formation by the osteoblast cells. Little is known about the role that small (approximately 22 nucleotide), non-coding micro-RNAs (miRNAs) play in the osteoblast response to microgravity. We hypothesize that osteoblast-lineage cells alter their miRNA status during microgravity exposure, contributing to impaired bone formation during weightlessness. To simulate weightlessness, female mice (C57BL/6, Charles River, 10 weeks of age, n = 6) were hindlimb unloaded for 12 days. Age-matched and normally ambulating mice served as controls (n=6). To assess the expression of miRNAs in skeletal tissue, the right and left tibia of the mice were collected ex vivo and cleaned of soft-tissue and marrow. Total RNA was collected from tibial bone and relative abundance was measured for miRNAs of interest using quantitative real time PCR array looking at 372 unique and well-characterized mature miRNAs using the delta-delta Ct method. Transcripts of interest were normalized to an average of 6 reference RNAs. Preliminary results show that hindlimb unloading decreased the expression of 14 miRNAs to less than 1.4-2.9X control levels and increased the expression of 5 miRNAs relative to the control mice greater than 1-2-1.5X (p less than 0.05, respectively). Using the miRSystem we assessed overlapping target genes predicted to be regulated by multiple members of the 19 differentially expressed miRNAs as well as in silico predicted targets of our individual miRNAs. Our miRSystem results indicated that a number of our differentially expressed miRNAs were regulators of genes related to the Wnt-Beta Catenin pathway-a known regulator of bone health-and, interestingly, the estrogen-mediated cell-cycle regulation pathway, which may indicate that simulated weightlessness induced systemic hormonal changes that contributed to bone loss. We plan to follow up these findings by measuring gene expression of miRNA-regulated genes within these two pathways with the aim of furthering our understanding of the function of miRNAs in the skeletal response to spaceflight.

bone↗
NASA NTRS1977

Prediction of static aerodynamic characteristics for slender bodies alone and with lifting surfaces to very high angles of attack

An engineering-type method is presented for computing normal-force and pitching-moment coefficients for slender bodies of circular and noncircular cross section alone and with lifting surfaces. In this method, a semi-empirical term representing viscous-separation crossflow is added to a term representing potential-theory crossflow. For many bodies of revolution, computed aerodynamic characteristics are shown to agree with measured results for investigated free-stream Mach numbers from 0.6 to 2.9. The angles of attack extend from 0 deg to 180 deg for M = 2.9 from 0 deg to 60 deg for M = 0.6 to 2.0. For several bodies of elliptic cross section, measured results are also predicted reasonably well over the investigated Mach number range from 0.6 to 2.0 and at angles of attack from 0 deg to 60 deg. As for the bodies of revolution, the predictions are best for supersonic Mach numbers. For body-wing and body-wing-tail configurations with wings of aspect ratios 3 and 4, measured normal-force coefficients and centers are predicted reasonably well at the upper test Mach number of 2.0. Vapor-screen and oil-flow pictures are shown for many body, body-wing and body-wing-tail configurations. When spearation and vortex patterns are asymmetric, undesirable side forces are measured for the models even at zero sideslip angle. Generally, the side-force coefficients decrease or vanish with the following: increase in Mach number, decrease in nose fineness ratio, change from sharp to blunt nose, and flattening of body cross section (particularly the body nose).

Jorgensen, L. H.↗
NASA NTRS1982

Analysis of the nature and cause of turbulence upset using airline flight records

The development and application of methods for determining aircraft motions and related winds, using data normally recorded during airline flight operations, are described. The methods are being developed, in cooperation with the National Transportation Safety Board, to aid in the analysis and understanding of circumstances associated with aircraft accidents or incidents. Data from a recent DC-10 encounter with severe, high-altitude turbulence are used to illustrate the methods. The analysis of this encounter shows the turbulence to be a series of equally spaced horizontal swirls known as 'cat's eyes' vortices. The use of flight-data analysis methods to identify this type of turbulence phenomenon is presented for the first time.

Parks, E. K.↗
DOE OSTI2022

Multivariate Time Series Anomaly Detection with Few Positive Samples

Given the scarcity of anomalies in real-world applications, the majority of literature has been focusing on modeling normality. The learned representations enable anomaly detection as the normality model is trained to capture certain key underlying data regularities under normal circumstances. In practical settings, particularly industrial time series anomaly detection, we often encounter situations where a large amount of normal operation data is available along with a small number of anomaly events collected over time. This practical situation calls for methodologies to leverage these small number of anomaly events to create a better anomaly detector. In this paper, we introduce two methodologies to address the needs of this practical situation and compared them with recently developed state of the art techniques. Our proposed methods anchor on representative learning of normal operation with autoregressive (AR) model along with loss components to encourage representations that separate normal versus few positive examples. We applied the proposed methods to two industrial anomaly detection datasets and demonstrated effective performance in comparison with approaches from literature. Our study also points out additional challenges with adopting such methods in practical applications.

Xue, Feng↗
NASA NTRS2001

Engine With Regression and Neural Network Approximators Designed

At the NASA Glenn Research Center, the NASA engine performance program (NEPP, ref. 1) and the design optimization testbed COMETBOARDS (ref. 2) with regression and neural network analysis-approximators have been coupled to obtain a preliminary engine design methodology. The solution to a high-bypass-ratio subsonic waverotor-topped turbofan engine, which is shown in the preceding figure, was obtained by the simulation depicted in the following figure. This engine is made of 16 components mounted on two shafts with 21 flow stations. The engine is designed for a flight envelope with 47 operating points. The design optimization utilized both neural network and regression approximations, along with the cascade strategy (ref. 3). The cascade used three algorithms in sequence: the method of feasible directions, the sequence of unconstrained minimizations technique, and sequential quadratic programming. The normalized optimum thrusts obtained by the three methods are shown in the following figure: the cascade algorithm with regression approximation is represented by a triangle, a circle is shown for the neural network solution, and a solid line indicates original NEPP results. The solutions obtained from both approximate methods lie within one standard deviation of the benchmark solution for each operating point. The simulation improved the maximum thrust by 5 percent. The performance of the linear regression and neural network methods as alternate engine analyzers was found to be satisfactory for the analysis and operation optimization of air-breathing propulsion engines (ref. 4).

Patnaik, Surya N.↗
NASA NTRS1983

Multiple spacecraft observations of interplanetary shocks: Characteristics of the upstream ULF turbulence

All interplanetary shocks observed by ISEE-3 and either ISEE-1 or ISEE-2 or both in 1978 and 1979 are examined for evidence of upstream waves. In order to characterize the properties of these shocks it is necessary to determine accurate shock normals. An overdetermined set of equations were inverted to obtain shock normals, velocities and error estimates for all these shocks. Tests of the method indicate it is quite reliable. Using these normals the Mach number and angle were between the interplanetary magnetic field and the shock normal for each shock. The upstream waves were separated into two classes: whistler mode precursors which occur at low Mach numbers and upstream turbulence whose amplitude at Mach numbers greater than 1.5 is controlled by the angle of the field to the shock normal. The former waves are right hand circularly polarized and quite monochromatic. The latter waves are more linearly polarized and have a broadband featureless spectrum.

Russell, C. T.↗
NASA NTRS1994

Aerodynamics Characteristics of Multi-Element Airfoils at -90 Degrees Incidence

A developed method has been applied to calculate accurately the viscous flow about airfoils normal to the free-stream flow. This method has special application to the analysis of tilt rotor aircraft in the evaluation of download. In particular, the flow about an XV-15 airfoil with and without deflected leading and trailing edge flaps at -90 degrees incidence is evaluated. The multi-element aspect of the method provides for the evaluation of slotted flap configurations which may lead to decreased drag. The method solves for turbulent flow at flight Reynolds numbers. The flow about the XV-15 airfoil with and without flap deflections has been calculated and compared with experimental data at a Reynolds number of one million. The comparison between the calculated and measured pressure distributions are very good, thereby, verifying the method. The aerodynamic evaluation of multielement airfoils will be conducted to determine airfoil/flap configurations for reduced airfoil drag. Comparisons between the calculated lift, drag and pitching moment on the airfoil and the airfoil surface pressure will also be presented.

Stremel, Paul M.↗
NASA NTRS2004

The effect of simulated microgravity on bacteria from the Mir space station

The effects of simulated microgravity on two bacterial isolates, Sphingobacterium thalpophilium and Ralstonia pickettii (formerly Burkholderia pickettii), originally recovered from water systems aboard the Mir space station were examined. These bacteria were inoculated into water, high and low concentrations of nutrient broth and subjected to simulated microgravity conditions. S. thalpophilium (which was motile and had flagella) showed no significant differences between simulated microgravity and the normal gravity control regardless of the method of enumeration and medium. In contrast, for R. pickettii (that was non-motile and lacked flagella), there were significantly higher numbers in high nutrient broth under simulated microgravity compared to normal gravity. Conversely, when R. pikkettii was inoculated into water (i.e., starvation conditions) significantly lower numbers were found under simulated microgravity compared to normal gravity. Responses to microgravity depended on the strain used (e.g., the motile strain exhibited no response to microgravity, while the non-motile strain did), the method of enumeration, and the nutrient concentration of the medium. Under oligotrophic conditions, non-motile cells may remain in geostationary orbit and deplete nutrients in their vicinity, while in high nutrient medium, resources surrounding the cell may be sufficient so that high growth is observed until nutrients becoming limiting.

Non-NASA Center↗
NASA NTRS1997

A New Approach to Extreme Value Estimation Applicable to a Wide Variety of Random Variables

Designing reliable structures requires an estimate of the maximum and minimum values (i.e., strength and load) that may be encountered in service. Yet designs based on very extreme values (to insure safety) can result in extra material usage and hence, uneconomic systems. In aerospace applications, severe over-design cannot be tolerated making it almost mandatory to design closer to the assumed limits of the design random variables. The issue then is predicting extreme values that are practical, i.e. neither too conservative or non-conservative. Obtaining design values by employing safety factors is well known to often result in overly conservative designs and. Safety factor values have historically been selected rather arbitrarily, often lacking a sound rational basis. To answer the question of how safe a design needs to be has lead design theorists to probabilistic and statistical methods. The so-called three-sigma approach is one such method and has been described as the first step in utilizing information about the data dispersion. However, this method is based on the assumption that the random variable is dispersed symmetrically about the mean and is essentially limited to normally distributed random variables. Use of this method can therefore result in unsafe or overly conservative design allowables if the common assumption of normality is incorrect.

Holland, Frederic A., Jr.↗
NASA NTRS1974

Electromagnetic fields due to a horizontal electric dipole antenna laid on the surface of a two-layer medium

With applications to geophysical subsurface probings, electromagnetic fields due to a horizontal electric dipole laid on the surface of a two-layer medium are solved by a combination of analytic and numerical methods. Interference patterns are calculated for various layer thickness. The results are interpreted in terms of normal modes, and the accuracies of the methods are discussed.

Tsang, L.↗
NASA NTRS2010

Influence of Averaging Method on the Evaluation of a Coastal Ocean Color Event on the U.S. Northeast Coast

Application of appropriate spatial averaging techniques is crucial to correct evaluation of ocean color radiometric data, due to the common log-normal or mixed log-normal distribution of these data. Averaging method is particularly crucial for data acquired in coastal regions. The effect of averaging method was markedly demonstrated for a precipitation-driven event on the U.S. Northeast coast in October-November 2005, which resulted in export of high concentrations of riverine colored dissolved organic matter (CDOM) to New York and New Jersey coastal waters over a period of several days. Use of the arithmetic mean averaging method created an inaccurate representation of the magnitude of this event in SeaWiFS global mapped chl a data, causing it to be visualized as a very large chl a anomaly. The apparent chl a anomaly was enhanced by the known incomplete discrimination of CDOM and phytoplankton chlorophyll in SeaWiFS data; other data sources enable an improved characterization. Analysis using the geometric mean averaging method did not indicate this event to be statistically anomalous. Our results predicate the necessity of providing the geometric mean averaging method for ocean color radiometric data in the Goddard Earth Sciences DISC Interactive Online Visualization ANd aNalysis Infrastructure (Giovanni).

Acker, James G.↗
DOE OSTI2026

Demystifying Piecewise and Localized Scatter Correction Methods

Multiplicative scatter is a common source of noise in near-infrared spectroscopy and other related instrumental techniques. A wide variety of methods are commonly used for the correction of multiplicative scatter. However, the majority of such methods assume that the parameters that describe the scatter are constant throughout the measured spectrum, which is often not the case. This work investigates a family of methods that perform scatter correction using local regions of neighboring wavelengths in order to better account for wavelength-dependent scattering. The methods in question are piecewise standard normal variate, localized standard normal variate, piecewise multiplicative scatter correction, and localized multiplicative scatter correction. This work describes the theoretical and algorithmic foundations of the family of local region-based scatter correction methods and compares their application and optimization at a qualitative and quantitative level using several datasets.

NIR spectroscopy↗
NASA NTRS1980

A correlation method to predict the surface pressure distribution of an infinite plate or a body of revolution from which a jet is issuing

A correlation method to predict pressures induced on an infinite plate by a jet exhausting normal to the plate into a subsonic free stream was extended to jets exhausting at angles to the plate and to jets exhausting normal to the surface of a body revolution. The complete method consisted of an analytical method which models the blockage and entrainment properties of the jet and an empirical correlation which accounts for viscous effects. For the flat plate case, the method was applicable to jet velocity ratios up to ten, jet inclination angles up to 45 deg from the normal, and radial distances up to five diameters from the jet. For the body of revolution case, the method was applicable to a body at zero degrees angle of attack, jet velocity ratios 1.96 and 3.43, circumferential angles around the body up to 25 deg from the jet, axial distances up to seven diameters from the jet, and jet-to-body diameter ratios less than 0.1.

Perkins, S. C., Jr.↗
NASA NTRS1987

Probabilistic load model development and validation for composite load spectra for select space propulsion engines

A major task of the program to develop an expert system to predict the loads on selected components of a generic space propulsion engine is the design development and application of a probabilitic loads model. This model is being developed in order to account for the random nature of the loads and assess the variable load ranges' effect on the engine performance. A probabilistic model has been developed. The model is based primarily on simulation methods, but also has a Gaussian algebra method (if all variables are near normal), a fast probability integrator routine (for the calculation of low probability events), and a separate, stand alone program for performing barrier crossing calculations. Each of these probabilistic methods has been verified with theoretical calculations using assumed distributional forms.

Kurth, R.↗