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At least 379 records · Page 21

Growth Curve Parameterization of Metabolic Activity of Yeast Cells for BioSentinel

The goal of the BioSentinel small satellite payload is to measure the effect of deep space radiation on the growth and metabolic activity of yeast cells. Raw test data is generated by fluidics cards containing yeast cells rehydrated at different periods, with metabolic activity measured by the reduction of alamarBlue. Each card well has a sensor array that measures the amount of red, green, and infrared light transmitted through the yeast culture. This illumination data is then converted to absorbance values, which are further converted into concentrations. The ultimate objective is to convert these concentrations into biologically-relevant metrics that can be compared against one another to determine changes due to differential radiation exposure. Beginning with IR absorbance data (corresponding to cell density) from ground studies, three parameters from a sigmoidal growth curve were extracted and analyzed: 𝜆 (lag phase), 𝜇 (max growth rate), and A (max cell growth). The data was fit to the Gompertz model of microbial growth using non-linear regression (Minitab), as the fit error was reduced compared to the simpler logistic growth curve. Graphs showed that the data contained a discrepancy (drift) in the lag phase that is attributable to a slow, constant loss of moisture. Correcting this discrepancy by fitting the first 25 hours of the data to a power function and subtracting these values from the absorbance readings obtained a better statistical fit to the growth curve in the lag phase. A power fit was selected over a linear fit because it reflected the effects of constant volume loss. This correction to the BioSentinel data analysis pipeline will enable quantitative statistical analysis of the effect of different levels of deep space radiation on yeast cells. Future work includes automation of drift correction and curve modeling to extract these parameters directly from data.

Growth Curve↗

VEG: An intelligent workbench for analysing spectral reflectance data

An Intelligent Workbench (VEG) was developed for the systematic study of remotely sensed optical data from vegetation. A goal of the remote sensing community is to infer the physical and biological properties of vegetation cover (e.g. cover type, hemispherical reflectance, ground cover, leaf area index, biomass, and photosynthetic capacity) using directional spectral data. VEG collects together, in a common format, techniques previously available from many different sources in a variety of formats. The decision as to when a particular technique should be applied is nonalgorithmic and requires expert knowledge. VEG has codified this expert knowledge into a rule-based decision component for determining which technique to use. VEG provides a comprehensive interface that makes applying the techniques simple and aids a researcher in developing and testing new techniques. VEG also provides a classification algorithm that can learn new classes of surface features. The learning system uses the database of historical cover types to learn class descriptions of one or more classes of cover types.

Harrison, P. Ann↗

Analysis of counting errors in the phase/Doppler particle analyzer

NASA is investigating the application of the Phase Doppler measurement technique to provide improved drop sizing and liquid water content measurements in icing research. The magnitude of counting errors were analyzed because these errors contribute to inaccurate liquid water content measurements. The Phase Doppler Particle Analyzer counting errors due to data transfer losses and coincidence losses were analyzed for data input rates from 10 samples/sec to 70,000 samples/sec. Coincidence losses were calculated by determining the Poisson probability of having more than one event occurring during the droplet signal time. The magnitude of the coincidence loss can be determined, and for less than a 15 percent loss, corrections can be made. The data transfer losses were estimated for representative data transfer rates. With direct memory access enabled, data transfer losses are less than 5 percent for input rates below 2000 samples/sec. With direct memory access disabled losses exceeded 20 percent at a rate of 50 samples/sec preventing accurate number density or mass flux measurements. The data transfer losses of a new signal processor were analyzed and found to be less than 1 percent for rates under 65,000 samples/sec.

Oldenburg, John R.↗

Static Aerodynamics of the Mars Exploration Rover Entry Capsule

The static aerodynamics for the Mars Exploration Rover (MER) aeroshell are presented. This aerodynamic database was an integral part of the end-to-end simulation used in pre- entry analysis for determining the MER entry design requirements for development of the MER entry system, as well as targeting the MER landing sites. The database was constructed using the same approach used for Mars Pathfinder (MPF). However, the MER aerodynamic database is of much higher fidelity and tailored to the MER entry trajectories. This set of data includes direct simulation Monte Carlo calculations covering the transitional regime of the entry trajectory and computational fluid dynamics calculations describing the aerodynamics in the hypersonic and supersonic continuum regimes. An overview of the methodology used to generate the data is given along with comparisons to important features in the MPF aerodynamics and related heritage data. The MER and MPF comparison indicates that trajectory specific data is required to properly model the flight characteristics of a.blunt entry capsule at Mars.

Schoenenberger, Mark↗

Planform effects on the supersonic aerodynamics of multibody configurations

An experimental and theoretical investigation of the effect of planform on the supersonic aerodynamics of low-fineness-ratio multibody configurations was conducted. Longitudinal and lateral-directional aerodynamic and flow visualization data were obtained on three multibody configurations. The data indicated that planform has a small effect on the zero lift drag of a multibody configuration. The longitudinal data obtained at lifting conditions showed a sensitivity to planform shape. Lateral-directional data obtained for all configurations did not uncover any unusual stability traits for this class of configuration. A comparison study was also made between the planform effects observed on single-body and multibody configurations. Results from this study indicate that the multibody concept appears to offer a mechanism for employing a low-sweep wing with no significant increase in zero-lift drag but still retaining high-performance characteristics at high-lift conditions. Evaluation of the linear-theory prediction methods revealed a general inability of the methods to predict the characteristics of low-fineness-ratio geometries.

Mcmillin, Naomi↗

A Comparison of Expedition Medical Condition List Treatment Directives with Integrated Medical Model Simulation Data Presentation and Briefing Report

This aerospace medicine clerkship project is under the direction of Dr. Sharmila Watkins and is in cooperation with Dr. Eric Kerstman and Dr. Ronak Shah. The questions of the research project are: 1. What are the main drivers of evacuation and loss of crew life (LOCL) on three Design Reference Missions (DRMs): Near Earth Asteroid (NEA), Lunar Sortie and Lunar Outpost using an inexhaustible International Space Station medical kit 2. What are the treatment designations for these driving medical conditions as listed in Expedition Medical Condition List (EMCL) 3. Do the drivers make sense in the context of the given Design Reference Mission (DRM) 4. Do any EMCL treatment designations need re-assessing.

Lewis, Robert↗

Estimates of the geostrophic stream function in the western North Pacific from XBT surveys

Bernstein and White (1981) have argued that the 300 m temperature field in the western North Pacific reflects the 100/1000 dbar geostrophic stream function of the upper ocean where 100/1000 dbar implies the 100 dbar surface relative to the 1000 dbar surface. The correlation between upper ocean dynamic height and temperature in this region provides the basis for their argument. The involved relationship suggests that it may be possible to monitor the relative upper ocean geostrophic transport, as well as energy relations and heat fluxes with expendable bathythermograph (XBT) surveys. Certain discrepancies between data from different sources are felt to result from problems with the XBT observations. The present investigation is concerned with the determination of the ability of XBT surveys to estimate geostrophic currents, taking into account a comparison of directly measured data with estimated shears. Discrepancies are found to be related to an underestimation of the dynamic height gradients.

Koblinsky, C. J.↗

Testing the Archivas Cluster (Arc) for Ozone Monitoring Instrument (OMI) Scientific Data Storage

The Ozone Monitoring Instrument (OMI) launched on NASA's Aura Spacecraft, the third of the major platforms of the EOS program on July 15,2004. In addition to the long term archive and distribution of the data from OM1 through the Goddard Earth Science Distributed Active Archive Center (GESDAAC), we are evaluating other archive mechanisms that can archive the data in a more immediately available method where it can be used for futher data production and analysis. In 2004, Archivas, Inc. was selected by NASA s Small Business Innovative Research (SBIR) program for the development of their Archivas Cluster (ArC) product. Arc is an online disk based system utilizing self-management and automation on a Linux cluster. Its goal is to produce a low cost solution coupled with the ease of management. The OM1 project is an application partner of the SBIR program, and has deployed a small cluster (5TB) based on the beta Archwas software. We performed extensive testing of the unit using production OM1 data since launch. In 2005, Archivas, Inc. was funded in SBIR Phase II for further development, which will include testing scalability with the deployment of a larger (35TB) cluster at Goddard. We plan to include Arc in the OM1 Team Leader Computing Facility (TLCF) hosting OM1 data for direct access and analysis by the OMI Science Team. This presentation will include a brief technical description of the Archivas Cluster, a summary of the SBIR Phase I beta testing results, and an overview of the OMI ground data processing architecture including its interaction with the Phase II Archivas Cluster and hosting of OMI data for the scientists.

Tilmes, Curt↗

Developing Flexible Instruments for Biological Missions Beyond Low Earth Orbit

As the future of spaceflight focuses on human exploration beyond low Earth orbit (BLEO), space biology experiments using model organisms are becoming increasingly important. NASA’s Artemis missions seek to build technologies that enable extended crewed flights into deep space, a region not travelled by humans for over 50 years. From the Apollo missions and ground experiments, it is known that BLEO galactic cosmic radiation can cause damage to DNA and proteins, as well as an increased risk of cancer to astronauts. NASA’s latest biosensor technology, the Lunar Explorer Instrument for space biology applications (LEIA), builds upon the viable and cost-effective platform of CubeSats to take biology experiments back to the Moon. Stationed on the lunar South Pole, LEIA will collect valuable in-situ radiation data, and use yeast to study the response to combined partial gravity and radiation stressors, as well as provide a proof-of-concept of on-demand bio nutrient production as a countermeasure for future crewed missions. Directly enhancing the abilities of the BioSentinel CubeSat mission, the main components of LEIA are the two radiation sensors and the 4U BioSensor, composed of 16-microfluidic cards housing dried yeast cells. The payload is fully contained and autonomous, directly sending data back to Earth without the need for sample return. In addition, the thermal environment of the BioSensor is optimized to keep cells alive for the pre-launch period and duration of the Artemis III mission, while also running on limited power supply. This talk highlights how the development of flexible instruments, like LEIA, enables human exploration into deep space. The technology developed with LEIA can be used as a stepping-stone for establishing a sustained lunar surface habitation and beyond, as humans continue to venture into space.

Payne Elizabeth Turney↗

Investigation of vehicle glow in the far ultraviolet

To data, all vehicle glow observations have been conducted in the visible and near infrared wavelength regions. As the Space Telescope's wavelength coverage extends to the far ultraviolet range and current plasma theory of the spacecraft glow phenomena predicts bright glow intensities, a study of the ram glow effects in the 800-1400 angstrom region was undertaken. The data were collected between March 21-28, 1979, from 600 km altitude near local midnight by the University of California, Berkeley's extreme ultraviolet spectrometer on board the polar orbiting STP78-1 satellite. Data from several nighttime orbits obtained outside the South Atlantic Anomaly region and within + or - 30 deg magnetic latitude range were separated into forward (south viewing) and backward (north viewing) bins. Each of these binds was subdivided into three directional categories: (1) up (zenith angles 30-80 deg), (2) side (zenith angles 80-100 deg), and (3) down (zenith angles 120-150 deg). The maximum ram glow effects are expected in the side viewing directions. Our data include possible effects of ram glow signatures in the 800-1400 angstrom wavelength region.

Chakrabarti, S.↗

Applications of ISES for the oceans

The need in oceanography for direct broadcast, why, and what type are discussed. Four reasons for support exist: need for real-time observations in support of field studies; need to back up point failures; need for experimentation with onboard processing and selection of data for direct broadcasting; and the need to develop future operational NOAA systems. Each of these areas is examined.

Esaias, W. E.↗

Infrared and Raman spectroscopic studies of structural variations in minerals from Apollo 11, 12, 14 and 15 samples, volume 3

Infrared and Raman vibrational spectroscopic data, yielding direct information on molecular structure, were obtained for single grains ( 150 microns) of minerals, basalts, and glasses isolated from Apollo 11, 12, 14, and 15 rock and dust samples, and for grains in Apollo 14 polished butt samples. From the vibrational data, specification substitutions were determined for the predominant silicate minerals of plagioclase, pyroxene, and olivine. Unique spectral variations for grains of K-feldspar, orthopyroxene, pyroxenoid, and ilmenite were observed to exceed the ranges of terrestrial samples, and these variations may be correlatable with formation histories. Alpha-quartz was isolated as pure single grains, in granitic grains composited with sanidine, and in unique grains that were intimately mixed with varying amounts of glass. Accessory minerals of chromite and ulvospinel were isolated as pure grains and structurally characterized from their distinctive infrared spectra. Fundamental vibrations of the SiO4 tetrahedra in silicate minerals were used to classify bulk compositions in dust sieved fractions, basalt grains and glass particles, and to compare modal characteristics for maria, highland and rille samples. No hydrated minerals were found in any of the samples studied, indicating anhydrous formation conditions.

Estep, P. A.↗

Analysis of Rawinsonde Spatial Separation for Space Launch Vehicle Applications at the Eastern Range

Spatial separation of HR rawinsonde data is directly correlated with climatological tropospheric wind environment over ER. Stronger winds in the winter result in further downrange drift. Lighter winds in the summer result in the less horizontal drift during ascent. Maximum downrange distance can exceed 200 km during winter months. Data could misrepresent the environment the vehicle will experience during ascent. PRESTO uses all available data sources to produce the best representative, vertically complete atmosphere for launch vehicle DOL operations. Capability planned for use by NASA Space Launch System vehicle's first flight scheduled for Fall 2018.

Decker, Ryan K.↗

Wind Tunnel Results of the Aerodynamic Performance of a 1/8-Scale Model of a Twin-Engine Transport with Multi-Element Wing

A wind tunnel investigation was performed in the 14- by 22-Foot Subsonic Tunnel on a pressure instrumented 1/8-scale twin-engine subsonic transport to better understand the flow physics on a multi-element wing section. The wing consisted of a part-span, triple-slotted trailing edge flap, inboard leading-edge Krueger flap and an outboard leading-edge slat. The model was instrumented with flush pressure ports at the fuselage centerline and seven spanwise wing locations. The model was tested in cruise, take-off and landing configurations at dynamic pressures and Mach numbers from 10 lbf/ft(exp 2) to 50 lbf/ft(exp 2) and 0.08 to 0.17, respectively. This resulted in corresponding Reynolds numbers of 0.8 x 10(exp 5) to 1.8 x 10(exp 6). Pressure data were collected using electronically scanned pressure devices and force and moment data were collected with a six component strain gauge balance. Results are presented for various control surface deflections over an angle-of-attack range from -4 degrees to 16 degrees and sideslip angle range from -10 degrees to 10 degrees. Longitudinal and lateral directional aerodynamic data are presented as well as chordwise pressure distributions at the seven spanwise wing locations and the fuselage centerline.

Laflin, Brenda E. Gile↗

An Ensemble Approach to Building Mercer Kernels with Prior Information

This paper presents a new methodology for automatic knowledge driven data mining based on the theory of Mercer Kernels, which are highly nonlinear symmetric positive definite mappings from the original image space to a very high, possibly dimensional feature space. we describe a new method called Mixture Density Mercer Kernels to learn kernel function directly from data, rather than using pre-defined kernels. These data adaptive kernels can encode prior knowledge in the kernel using a Bayesian formulation, thus allowing for physical information to be encoded in the model. Specifically, we demonstrate the use of the algorithm in situations with extremely small samples of data. We compare the results with existing algorithms on data from the Sloan Digital Sky Survey (SDSS) and demonstrate the method's superior performance against standard methods. The code for these experiments has been generated with the AUTOBAYES tool, which automatically generates efficient and documented C/C++ code from abstract statistical model specifications. The core of the system is a schema library which contains templates for learning and knowledge discovery algorithms like different versions of EM, or numeric optimization methods like conjugate gradient methods. The template instantiation is supported by symbolic-algebraic computations, which allows AUTOBAYES to find closed-form solutions and, where possible, to integrate them into the code.

Srivastava, Ashok N.↗

Putting Priors in Mixture Density Mercer Kernels

This paper presents a new methodology for automatic knowledge driven data mining based on the theory of Mercer Kernels, which are highly nonlinear symmetric positive definite mappings from the original image space to a very high, possibly infinite dimensional feature space. We describe a new method called Mixture Density Mercer Kernels to learn kernel function directly from data, rather than using predefined kernels. These data adaptive kernels can en- code prior knowledge in the kernel using a Bayesian formulation, thus allowing for physical information to be encoded in the model. We compare the results with existing algorithms on data from the Sloan Digital Sky Survey (SDSS). The code for these experiments has been generated with the AUTOBAYES tool, which automatically generates efficient and documented C/C++ code from abstract statistical model specifications. The core of the system is a schema library which contains template for learning and knowledge discovery algorithms like different versions of EM, or numeric optimization methods like conjugate gradient methods. The template instantiation is supported by symbolic- algebraic computations, which allows AUTOBAYES to find closed-form solutions and, where possible, to integrate them into the code. The results show that the Mixture Density Mercer-Kernel described here outperforms tree-based classification in distinguishing high-redshift galaxies from low- redshift galaxies by approximately 16% on test data, bagged trees by approximately 7%, and bagged trees built on a much larger sample of data by approximately 2%.

Srivastava, Ashok N.↗

Experimental Investigation of Rotating Stall in a Research Multistage Axial Compressor

A collection of experimental data acquired in the NASA low-speed multistage axial compressor while operated in rotating stall is presented in this paper. The compressor was instrumented with high-response wall pressure modules and a static pressure disc probe for in-flow measurement, and a split-fiber probe for simultaneous measurements of velocity magnitude and flow direction. The data acquired to-date have indicated that a single fully developed stall cell rotates about the flow annulus at 50.6% of the rotor speed. The stall phenomenon is substantially periodic at a fixed frequency of 8.29 Hz. It was determined that the rotating stall cell extends throughout the entire compressor, primarily in the axial direction. Spanwise distributions of the instantaneous absolute flow angle, axial and tangential velocity components, and static pressure acquired behind the first rotor are presented in the form of contour plots to visualize different patterns in the outer (midspan to casing) and inner (hub to mid-span) flow annuli during rotating stall. In most of the cases observed, the rotating stall started with a single cell. On occasion, rotating stall started with two emerging stall cells. The root cause of the variable stall cell count is unknown, but is not attributed to operating procedures.

Lepicovsky, Jan↗

Remote-sensing based technique to account for sub-grid scale variability of land surface properties

A method has been presented for the representation of sub-grid scale variability of surface properties within a land surface processes model. The method uses remotely-sensed data to directly or indirectly estimate probability density functions (PDF's) or key surface variables. Application of this technique in a coupled land surface-atmosphere model requires only grid-scale values of the variables of interest, obtained from low-resolution satellite imagery or surface/remote sensing data assimilation. The PDF's of each controlling surface property are superimposed on the respective grid-scale values to simulate sub-grid scale heterogeneity. Sensitivity studies will be carried out to ascertain the relative importance of the heterogeneity of several variables, and the degree to which non-linear property-process interactions impact large-scale fluxes.

Crosson, William L.↗