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At least 379 records · Page 21
All sourcesNASA NTRSDOE OSTI
NASA NTRS1999

The Thin Oil Film Equation

A thin film of oil on a surface responds primarily to the wall shear stress generated on that surface by a three-dimensional flow. The oil film is also subject to wall pressure gradients, surface tension effects and gravity. The partial differential equation governing the oil film flow is shown to be related to Burgers' equation. Analytical and numerical methods for solving the thin oil film equation are presented. A direct numerical solver is developed where the wall shear stress variation on the surface is known and which solves for the oil film thickness spatial and time variation on the surface. An inverse numerical solver is also developed where the oil film thickness spatial variation over the surface at two discrete times is known and which solves for the wall shear stress variation over the test surface. A One-Time-Level inverse solver is also demonstrated. The inverse numerical solver provides a mathematically rigorous basis for an improved form of a wall shear stress instrument suitable for application to complex three-dimensional flows. To demonstrate the complexity of flows for which these oil film methods are now suitable, extensive examination is accomplished for these analytical and numerical methods as applied to a thin oil film in the vicinity of a three-dimensional saddle of separation.

Brown, James L.↗
NASA NTRS2012

Adhesive-Bonded Composite Joint Analysis with Delaminated Surface Ply Using Strain-Energy Release Rate

This paper presents an analytical model to determine the strain energy release rate due to an interlaminar crack of the surface ply in adhesively bonded composite joints subjected to axial tension. Single-lap shear-joint standard test specimen geometry with thick bondline is followed for model development. The field equations are formulated by using the first-order shear-deformation theory in laminated plates together with kinematics relations and force equilibrium conditions. The stress distributions for the adherends and adhesive are determined after the appropriate boundary and loading conditions are applied and the equations for the field displacements are solved. The system of second-order differential equations is solved to using the symbolic computation tool Maple 9.52 to provide displacements fields. The equivalent forces at the tip of the prescribed interlaminar crack are obtained based on interlaminar stress distributions. The strain energy release rate of the crack is then determined by using the crack closure method. Finite element analyses using the J integral as well as the crack closure method are performed to verify the developed analytical model. It has been shown that the results using the analytical method correlate well with the results from the finite element analyses. An attempt is made to predict the failure loads of the joints based on limited test data from the literature. The effectiveness of the inclusion of bondline thickness is justified when compared with the results obtained from the previous model in which a thin bondline and uniform adhesive stresses through the bondline thickness are assumed.

Chadegani, Alireza↗
NASA NTRS2008

Using Simulation Speeds to Differentiate Controller Interface Concepts

This study investigated two concepts: (1) whether speeding a human-in-the-loop simulation (or the subject's "world") scales time stress in such a way as to cause primary task performance to reveal workload differences between experimental conditions and (2) whether using natural hand motions to control the attitude of an aircraft makes controlling the aircraft easier and more intuitive. This was accomplished by having pilots and non-pilots make altitude and heading changes using three different control inceptors at three simulation speeds. Results indicate that simulation speed does affect workload and controllability. The bank and pitch angle error was affected by simulation speed but not by a simulation speed by controller type interaction; this may have been due to the relatively easy flying task. Results also indicate that pilots could control the bank and pitch angle of an aircraft about equally as well with the glove as with the sidestick. Non-pilots approached the pilots ability to control the bank and pitch angle of an aircraft using the positional glove - where the hand angle is directly proportional to the commanded aircraft angle. Therefore, (1) changing the simulation speed lends itself to objectively indexing a subject s workload and may also aid in differentiating among interface concepts based upon performance if the task being studied is sufficiently challenging and (2) using natural body movements to mimic the movement of an airplane for attitude control is feasible.

Trujillo, Anna↗
DOE OSTI2021

Peripheral Blood Transcript Signatures after Internal 131 I-mIBG Therapy in Relapsed and Refractory Neuroblastoma Patients Identifies Early and Late Biomarkers of Internal 131 I Exposures

131 I-metaiodobenzylguanidine ( 131 I-mIBG) is a targeted radiation therapy developed for the treatment of advanced neuroblastoma. We have previously shown that this patient cohort can be used to predict absorbed dose associated with early 131 I exposure, 72 h after treatment. We now expand these studies to identify gene expression differences associated with 131 I-mIBG exposure 15 days after treatment. Total RNA from peripheral blood lymphocytes was isolated from 288 whole blood samples representing 59 relapsed or refractory neuroblastoma patients before and after 131 I-mIBG treatment. We found that several transcripts predictive of early exposure returned to baseline levels by day 15, however, selected transcripts did not return to baseline. At 72 h, all 17 selected pathway-specific transcripts were differentially expressed. Transcripts CDKN1A (P < 0.000001), FDXR (P < 0.000001), DDB2 (P < 0.000001), and BBC3 (P < 0.000001) showed the highest up-regulation at 72 h after 131 I-mIBG exposure, with mean log2 fold changes of 2.55, 2.93, 1.86 and 1.85, respectively. At day 15 after 131I-mIBG, 11 of the 17 selected transcripts were differentially expressed, with XPC, STAT5B, PRKDC, MDM2, POLH, IGF1R, and SGK1 displaying significant up-regulation at 72 h and significant down-regulation at day 15. Interestingly, transcripts FDXR (P = 0.01), DDB2 (P = 0.03), BCL2 (P = 0.003), and SESN1 (P < 0.0003) maintained differential expression 15 days after 131 I-mIBG treatment. These results suggest that transcript levels for DNA repair, apoptosis, and ionizing radiation-induced cellular stress are still changing by 15 days after 131 I-mIBG treatment. Our studies showcase the use of biodosimetry gene expression panels as predictive biomarkers following early (72 h) and late (15 days) internal 131 I exposure. Our findings also demonstrate the utility of our transcript panel to differentiate exposed from non-exposed individuals up to 15 days after exposure from internal 131 I.

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTAN↗
NASA NTRS1988

Cyclic uniaxial and biaxial hardening of type 304 stainless steel modeled by the viscoplasticity theory based on overstress

The isotropic theory of viscoplasticity based on overstress does not use a yield surface or a loading and unloading criterion. The inelastic strain rate depends on overstress, the difference between the stress and the equilibrium stress, and is assumed to be rate dependent. Special attention is paid to the modeling of elastic regions. For the modeling of cyclic hardening, such as observed in annealed Type 304 stainless steel, and additional growth law for a scalar quantity which represents the rate independent asymptotic value of the equilibrium stress is added. It is made to increase with inelastic deformation using a new scalar measure which differentiates between nonproportional and proportional loading. The theory is applied to correlate uniaxial data under two step amplitude loading including the effect of further hardening at the high amplitude and proportional and nonproportional cyclic loadings. Results are compared with corresponding experiments.

Yao, David↗
NASA NTRS1990

Thermal effects on fiber optic strain sensors embedded in graphite-epoxy composites

Smart structures deployed in low earth orbit will be exposed to a hostile environment which will include temperature extremes during each orbit as the platform moves from the day to the night side. The stresses due to thermal cycling may compromise the performance of embedded fiber optic strain sensors because of differential thermal expansion of the fiber and the composite material. The effects of elevated temperature and thermal cycling on the performance of a fiber optic strain sensor embedded in a graphite-epoxy tube have been investigated for temperatures from 65 to 220 F. The results indicate that the fiber optic strain sensor measurements correlate well with conventional resistance strain gages attached to the tube.

Rogowski, R. S.↗
NASA NTRS1992

Composite laminated shells under internal pressure

A theoretical study is conducted of the response of filament-wound composite shells under internal pressure; a system of sixth-order ordinary differential equations is obtained by means of the cylindrically anisotropic elasticity field equations and Lekhnitskii's (1963) stress functions. The general expressions for the stresses and displacements in the laminated composite shells under internal pressure are discussed. Attention is given to the influence of the degree of material anisotropy and fiber orientation on the axial and induced twisting deformation.

Yuan, F. G.↗
NASA NTRS1995

Bearing-Mounting Concept Accommodates Thermal Expansion

Pins or splines allow radial expansion without slippage. Design concept for mounting rotary bearing accommodates differential thermal expansion between bearing and any structure(s) to which bearing connected. Prevents buildup of thermal stresses by allowing thermal expansion to occur freely but accommodating expansion in such way not to introduce looseness. Pin-in-slot configuration also maintains concentricity.

Nespodzany, Robert↗
NASA NTRS2002

Three-Dimensional Postbuckling Analysis of Curved Beams

Presented here is a method of solving highly flexible curved beam undergoing huge static or quasi-static deformations. A geometrically exact beam theory based on the use of Jaumann stresses and strains and exact coordinate transformation is presented in terms of 17 first-order ordinary differential equations, and a multiple shooting method is used to solve the corresponding nonlinear two-point boundary value problems. The geometrically exact beam theory accounts far large rotations, large displacements, initial curvatures, extensionality, and transverse shear strains. Four examples are used to demonstrate this method, including a rotating clamped-free beam under the influence of gravity and centrifugal forces, an L-frame subjected to an in-plane tip load, a circular arch subjected to a concentrated load, and a clamped-hinged helical spring subjected to an axial displacement. Results show that the combination of the multiple shooting method and the geometrically exact beam theory works very well. Moreover, the obtained numerically exact solutions can be used to verify the accuracy of nonlinear finite element codes for nonlinear analysis of complex structures.

Pai, P. Frank↗
DOE OSTI2022

Phosphate Availability Modulates Root Exudate Composition and Rhizosphere Microbial Community in a Teosinte and a Modern Maize Cultivar

Domestication and breeding have affected interactions between plants and their microbiomes in ways that are only beginning to be understood but may have important implications for recruitment of rhizosphere microorganisms, particularly under stress conditions. We investigated the responses of a modern maize (Zea mays subsp. mays) cultivar and its wild relative, teosinte (Z. mays subsp. parviglumis), to different phosphate availabilities. We appraised responses of the plant-microbial holobiont to phosphate stresses by profiling root exudate metabolomes, and microbial communities in the root endosphere and rhizosphere. We also performed plate assays to quantify phosphate-solubilizing microorganisms from the rhizosphere. Although root exudate metabolite profiles were distinct between the teosinte and modern maize under high phosphate, both plants shifted exudate compositions in response to phosphate stress toward a common metabolite profile. Root and rhizosphere microbial communities also responded significantly to both plant type and the phosphate availability. A subset of bacterial and fungal taxa were differentially abundant under the different phosphate conditions, with each of the three conditions favoring different taxa. Both teosinte and maize rhizospheres harbored phosphate-solubilizing microorganisms under all growth conditions. These results suggest that the root exudation response to phosphate stress was conserved through the domestication of maize from teosinte, shifting exudation levels of specific metabolites. Although microbial communities also shifted, plate-based assays did not detect selective recruitment of phosphate solubilizers in response to phosphate availability.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS2000

The fluid dynamic and shear environment in the NASA/JSC rotating-wall perfused-vessel bioreactor

The rotating-wall perfused-vessel (RWPV) bioreactor, used for both microgravity and Earth-based cell science experiments, is characterized in terms of the fluid dynamic and fluid shear stress environment. A numerical model of the flow field is developed and verified with laser Doppler velocimeter measurements. The effects of changes in operating conditions, including rotation rates and fluid perfusion rates, are investigated with the numerical model. The operating conditions typically used for ground-based experiments (equal rotation of the inner and outer cylinders) leads to flow patterns with relatively poor mass distribution characteristics. Approximately 50% of the inlet-perfused fluid bypasses the bulk of the fluid volume and flows to the perfusion exit. For operating conditions typical in microgravity, small differential rotation rates between the inner and outer cylinders lead to greatly improved flow distribution patterns and very low fluid shear stress levels over a large percentage of the fluid volume. Differences in flow patterns for the different operating conditions are explored. Large differences in the hydrodynamic environments for operating conditions typical of true microgravity and ground-based "microgravity simulations" are demonstrated.

Bioreactors↗
NASA NTRS1973

Prior to failure extension of flaws in a rate sensitive Tresca solid.

Theoretical analysis shows that the amount of slow growth occurring prior to fracture in a plane stress tension specimen subjected to a subcritical stress intensity level is affected by the ductility and rheological sensitivity of the material, the rate of loading, the initial crack size, and the geometrical configuration of the test. These parameters enter the governing nonlinear differential equation which describes the quasi-static crack extension in a rate-sensitive Tresca solid. Examples of numerical integration are given and employed to predict the maximum fracture toughness attained in a plane stress tension test.

Wnuk, M. P.↗
NASA NTRS1987

Inflight application of three pilot workload measurement techniques

Three inflight techniques for workload measurement were tested in nine pilots flying the NASA Kuiper Airborne Observatory: subjective ratings, heart rate, and communication performance. The activities that contributed to the crew-member workload varied; the commander was responsible for aircraft control and navigation whereas the copilot handled communications. The three workload measures were found to provide different information. Pilot ratings of workload, effort, and stress were sensitive to variations in flight-related task demands across flight segments but did not reflect specific differences in the type of demands imposed on the commander and the copilot. The heart rate was sensitive to the differential impact of duties, being higher for the commander than for the copilot. The rate of communications per minute of flight proved to be the most sensitive indicator. It was related to workload, stress, effort rating, and average heart rate across flight segments.

Hart, Sandra G.↗
DOE OSTI2021

Cryptic genetic structure and copy-number variation in the ubiquitous forest symbiotic fungus Cenococcum geophilum

Ectomycorrhizal (ECM) fungi associated with plants constitute one of the most successful symbiotic interactions in forest ecosystems. ECM support trophic exchanges with host plants and are important factors for the survival and stress resilience of trees. However, ECM clades often harbour morpho-species and cryptic lineages, with weak morphological differentiation. How this relates to intraspecific genome variability and ecological functioning is poorly known. Here, we analysed 16 European isolates of the ascomycete Cenococcum geophilum, an extremely ubiquitous forest symbiotic fungus with no known sexual or asexual spore-forming structures but with a massively enlarged genome. We carried out whole-genome sequencing to identify single-nucleotide polymorphisms. We found no geographic structure at the European scale but divergent lineages within sampling sites. Evidence for recombination was restricted to specific cryptic lineages. Lineage differentiation was supported by extensive copy-number variation. Finally, we confirmed heterothallism with a single MAT1 idiomorph per genome. Synteny analyses of the MAT1 locus revealed substantial rearrangements and a pseudogene of the opposite MAT1 idiomorph. Our study provides the first evidence for substantial genome-wide structural variation, lineage-specific recombination and low continent-wide genetic differentiation in C. geophilum. Our study provides a foundation for targeted analyses of intra-specific functional variation in this major symbiosis.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1985

Differential-Coil Eddy-Current Material Sorter

Small metal or other electrically conductive parts of same shape but different composition quickly sorted with differential-coil eddy-current sorter. Developed to distinguish between turbine blades of different alloys, hardnesses, and residual stress, sorter generally applicable to parts of simple and complex shape.

Nummelin, J.↗
NASA NTRS1984

Biophysical mechanism of differential growth during gravitropism

A research project is described the goal of which is to determine the mechanism of gravitropic curvature in plant stems at the biophysical and the cellular level. The reorientation of plant organs under the influence of gravity is due to differential growth of the upper and lower sides of the organ. The rate of plant cell enlargement is governed by four biophysical parameters: (1) the extensibility of the cell wall; (2) the minimum stress in the cell wall required for wall expansion (the "yield threshold'); (3) the osmotic pressure difference between the cell contents and the water source; and (4) the hydraulic conductivity of the pathway for water uptake. Gravitropic response must involve differential alteration of one or more of these four parameters on the two sides of the growing organ. Each of these factors will be examined to assess the role it plays in gravitropism.

Cosgrove, D.↗
NASA NTRS1946

The Lagrangian Multiplier Method of Finding Upper and Lower Limits to Critical Stresses of Clamped Plates

The theory of Lagrangian multipliers is applied to the problem of finding both upper and lower limits to the true compressive buckling stress of a clamped rectangular plate. The upper and lower limits thus bracket the truss, which cannot be exactly found by the differential-equation approach. The procedure for obtaining the upper limit, which is believed to be new, presents certain advantages over the classical Raleigh-Rite method of finding upper limits. The theory of the lower-limit procedure has been given by Trefftz but, in the present application, the method differs from that of Trefftz in a way that makes it inherently more quickly convergent. It is expected that in other buckling problems and in some vibration problems problems the Lagrangian multiplier method finding upper and lower limits may be advantageously applied to the calculation of buckling stresses and natural frequencies.

Budiansky, Bernard↗
DOE OSTI2025

Partners in root nodule symbiosis respond uniquely to heavy metal stresses in a host genotype-dependent manner

Abstract The mutualistic symbiosis between legume roots and soil rhizobia culminates in the formation of root nodules, where nitrogen is fixed. Root nodule symbiosis is inhibited by heavy metal stress. In this study, we investigated the relative responses of the symbiotic partners to a non-essential heavy metal cadmium (Cd) and an essential heavy metal zinc (Zn) stress and identified patterns in gene expression. We performed dual transcriptomics in nodules, using theMedicago truncatula-Sinorhizobium melilotisymbiotic system. Phenotypes were measured in the wild-typeMedicago truncatulaand a mutant in anABCtransporter gene (Mtabcg36), which showed compromised nodule formation in control conditions and further after heavy metal treatment. We observed that the rhizobia were particularly sensitive to Zn in mutant nodules. The greatest degree of differential gene expression in the host plant were observed under Cd and Zn treatments in wild-type nodules. Most Cd-regulated host genes were also differentially regulated by Zn, revealing little discernment between an essential and a non-essential ion under increased exposure. Furthermore, the host response to both the stresses affected auxin and iron homeostasis genes in a host genotype-dependent manner. Our results suggested impaired cadmium export from the mutant nodules. These results have potential implications in agricultural management systems and bioremediation strategies.

Science & Technology - Other Topics↗