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At least 379 records · Page 21

Analysis of growth patterns during gravitropic curvature in roots of Zea mays by use of a computer-based video digitizer

A computer-based video digitizer system is described which allows automated tracking of markers placed on a plant surface. The system uses customized software to calculate relative growth rates at selected positions along the plant surface and to determine rates of gravitropic curvature based on the changing pattern of distribution of the surface markers. The system was used to study the time course of gravitropic curvature and changes in relative growth rate along the upper and lower surface of horizontally-oriented roots of maize (Zea mays L.). The growing region of the root was found to extend from about 1 mm behind the tip to approximately 6 mm behind the tip. In vertically-oriented roots the relative growth rate was maximal at about 2.5 mm behind the tip and declined smoothly on either side of the maximum. Curvature was initiated approximately 30 min after horizontal orientation with maximal (50 degrees) curvature being attained in 3 h. Analysis of surface extension patterns during the response indicated that curvature results from a reduction in growth rate along both the upper and lower surfaces with stronger reduction along the lower surface.

NASA Discipline Plant Biology↗

Computer-based video digitizer analysis of surface extension in maize roots: kinetics of growth rate changes during gravitropism

We used a video digitizer system to measure surface extension and curvature in gravistimulated primary roots of maize (Zea mays L.). Downward curvature began about 25 +/- 7 min after gravistimulation and resulted from a combination of enhanced growth along the upper surface and reduced growth along the lower surface relative to growth in vertically oriented controls. The roots curved at a rate of 1.4 +/- 0.5 degrees min-1 but the pattern of curvature varied somewhat. In about 35% of the samples the roots curved steadily downward and the rate of curvature slowed as the root neared 90 degrees. A final angle of about 90 degrees was reached 110 +/- 35 min after the start of gravistimulation. In about 65% of the samples there was a period of backward curvature (partial reversal of curvature) during the response. In some cases (about 15% of those showing a period of reverse bending) this period of backward curvature occurred before the root reached 90 degrees. Following transient backward curvature, downward curvature resumed and the root approached a final angle of about 90 degrees. In about 65% of the roots showing a period of reverse curvature, the roots curved steadily past the vertical, reaching maximum curvature about 205 +/- 65 min after gravistimulation. The direction of curvature then reversed back toward the vertical. After one or two oscillations about the vertical the roots obtained a vertical orientation and the distribution of growth within the root tip became the same as that prior to gravistimulation. The period of transient backward curvature coincided with and was evidently caused by enhancement of growth along the concave and inhibition of growth along the convex side of the curve, a pattern opposite to that prevailing in the earlier stages of downward curvature. There were periods during the gravitropic response when the normally unimodal growth-rate distribution within the elongation zone became bimodal with two peaks of rapid elongation separated by a region of reduced elongation rate. This occurred at different times on the convex and concave sides of the graviresponding root. During the period of steady downward curvature the elongation zone along the convex side extended farther toward the tip than in the vertical control. During the period of reduced rate of curvature, the zone of elongation extended farther toward the tip along the concave side of the root. The data show that the gravitropic response pattern varies with time and involves changes in localized elongation rates as well as changes in the length and position of the elongation zone. Models of root gravitropic curvature based on simple unimodal inhibition of growth along the lower side cannot account for these complex growth patterns.

NASA Discipline Plant Biology↗

Clinical use of three-dimensional video measurements of eye movements

Noninvasive measurements of three-dimensional eye position can be accurately achieved with video methods. A case study showing the potential clinical benefit of these enhanced measurements is presented along with some thoughts about technological advances, essential for clinical application, that are likely to occur in the next several years.

Non-NASA Center↗

A challenge for traditional video conferencing: HDTV?

In this paper we present an experiment in which we examine how life-size HDTV as a window connecting two conference rooms might overcome some of the problems found with using traditional video conferencing in meeting rooms across distance.

HDTV video conferencing↗

Quantitative Radiation Thermometry Using Commercially Available High-Speed Video Cameras

In order to understand the risk posed to astronauts by electric arc-generated particles, high-speed, high-resolution, quantitative thermal imaging was needed. The measurement requirements appeared to be beyond the capabilities of commercial thermal imaging systems, but the particles were known to have a significant amount of emission in the visible spectrum. This led to the use of commercially available, high-speed video cameras as imaging radiometers. Measured particle temperatures were consistent with predictions and other measurement data. The optical temperature measurement method, results, and conclusions are presented, along with recommendations for further work.

Thermal imaging↗

Association Between Patient Education Videos and Knowledge of Radiation Treatment

Patient knowledge of radiation therapy (RT) before consult is typically limited, with many having misconceptions or fears. There exists a need to improve patient education in RT. Our purpose was to study the impact of patient education videos on patient-reported knowledge of RT, anxiety/fear, and satisfaction.

62 RADIOLOGY AND NUCLEAR MEDICINE↗

Operando video microscopy of Li plating and re-intercalation on graphite anodes during fast charging

Despite the demand for fast-charging lithium (Li)-ion batteries, high-energy-density batteries with thick graphite anodes are limited by Li plating when charging at >4C rates. In this work, plan-view operando video microscopy is applied on >3 mA h cm –2 calendared graphite electrodes to study the dynamic evolution of local state-of-charge (SoC) and Li plating during fast charging. This technique allows for visualization of the spatial heterogeneity in SoC across the electrode, nucleation and growth of Li filaments, Li re-intercalation into graphite, “dead Li” formation, and SoC equilibration. The operando microscopy analysis is complemented by ex situ imaging of through-plane gradients in SoC to gain a three-dimensional visualization of spatial heterogeneity. We demonstrate that (1) Li plating preferentially nucleates on the graphite particles that lithiate fastest during fast charging; (2) the onset of Li plating correlates with the local minimum of the graphite electrode potential; (3) galvanic corrosion currents are responsible for Li re-intercalation, dead Li formation, and SoC re-equilibration after fast charging; and (4) electrochemical signatures during OCV rest or discharge are associated with Li re-intercalation into graphite. Furthermore, this work provides insight into the Li–graphite interactions at the composite electrode level and can be used to inform strategies to diagnose and mitigate Li plating during fast charging.

25 ENERGY STORAGE↗

Design and implementation of a prototype infrared video bolometer (IRVB) in MAST Upgrade

A prototype infrared video bolometer (IRVB) was successfully deployed in the Mega Ampere Spherical Tokamak Upgrade (MAST Upgrade or MAST-U), the first deployment of such a diagnostic in a spherical tokamak. The IRVB was designed to study the radiation around the lower x-point, another first in tokamaks, and has the potential to estimate emissivity profiles with spatial resolution beyond what is achievable with resistive bolometry. The system was fully characterized prior to installation on MAST-U, and the results are summarized here. After installation, it was verified that the actual measurement geometry in the tokamak qualitatively matches the design; this is a particularly difficult process for bolometers and was done using specific features of the plasma itself. The installed IRVB measurements are consistent both with observations from other diagnostics, including magnetic reconstruction, visible light cameras, and resistive bolometry, as well as with the IRVB-designed view. Early results show that with conventional divertor geometry and only intrinsic impurities (for example, C and He), the progression of radiative detachment follows a similar path to that observed for large aspect ratio tokamaks: The peak of the radiation moves along the separatrix from the targets to the x-point and high-field side midplane with a toroidally symmetric structure that can eventually lead to strong effects on the core plasma inside the separatrix.

47 OTHER INSTRUMENTATION↗

The infrared imaging video bolometer at Wendelstein 7-X

The radiated power distribution is a crucial aspect of heat transport, heat load mitigation, and plasma exhaust performance. An imaging video bolometer camera has been installed to measure the plasma radiated power in the divertor region of the Wendelstein 7-X (W7-X) stellarator. This diagnostic offers a wide-angle (40° × 68°) sampling of the plasma volume in both the poloidal and toroidal directions. The field-of-view is covered with a large number (> 500) of bolometer channels, providing imaging capability. The diagnostic design is introduced here together with its data analysis procedure. A set of laboratory experiments is performed to assess the thermal properties of the gold absorber foil and their spatial uniformity. Following installation, a heat source originating from the inertially cooled front of the diagnostic is identified and filtered out. The discharge data indicate a satisfactory signal-to-noise ratio as well as spatiotemporal resolution. These represent the first toroidally resolved images of the line-integrated radiated power in the W7-X island divertor. The diagnostic was then upgraded with a thinner platinum absorber and adjusted mirrors. Early data from the most recent experimental campaign employing the upgraded design show a considerable improvement in the diagnostic performance with more bolometer channels (> 1400), extended coverage, and increased spatial resolution.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Video-rate Raman-based metabolic imaging by Airy light-sheet illumination and photon-sparse detection

Despite its massive potential, Raman imaging represents just a modest fraction of all research and clinical microscopy to date. This is due to the ultralow Raman scattering cross-sections of most biomolecules that impose low-light or photon-sparse conditions. Bioimaging under such conditions is suboptimal, as it either results in ultralow frame rates or requires increased levels of irradiance. Here, we overcome this tradeoff by introducing Raman imaging that operates at both video rates and 1,000-fold lower irradiance than state-of-the-art methods. To accomplish this, we deployed a judicially designed Airy light-sheet microscope to efficiently image large specimen regions. Further, we implemented subphoton per pixel image acquisition and reconstruction to confront issues arising from photon sparsity at just millisecond integrations. We demonstrate the versatility of our approach by imaging a variety of samples, including the three-dimensional (3D) metabolic activity of single microbial cells and the underlying cell-to-cell variability. To image such small-scale targets, we again harnessed photon sparsity to increase magnification without a field-of-view penalty, thus, overcoming another key limitation in modern light-sheet microscopy.

60 APPLIED LIFE SCIENCES↗

High-resolution in-beam video rate imaging with the ClearXCam for synchrotron beamlines

The ClearXCam detector is a new video rate imaging in-beam monitor based on a diamond sensor. Imaging with 2304 effective pixels is achieved by sequentially biasing one of 48 metal stripes on one side of a diamond sensor, while reading out the current from 48 stripes on the other side of the sensor. This system was characterized for real-time X-ray beam diagnostics at synchrotron beam­line 17-BM at the National Synchrotron Light Source II. Significant results include: detection of in-beam structure via the imaging mode, beam focusing in one minute with real-time imaging feedback during a focusing event, linearity over five orders of magnitude, validation of a fast mode operating at 100 Hz, and sub-micron beam-positioning resolution. The system is now available commercially.

36 MATERIALS SCIENCE↗

Data, Photographs, Videos, and Information for the Niwot Ridge Subalpine Forest (US-NR1) AmeriFlux site

This data package contains data and information about the operation of the Niwot Ridge Subalpine Forest AmeriFlux site (US-NR1) between Nov 1998 to the present (2020). This data archive supplements the primary 30-min data storage for the US-NR1 data (i.e., https://doi.org/10.17190/AMF/1246088) by providing the following: (i) five-minute statistics (means, variances, covariances) of all data measured by the data system between Nov 1998 and September 2020 in netCDF format, (ii) CSV data files saved within the memory of the CR23X data loggers (as well as an archive of the data logger programs), (iii) an archive of previous 30-min ASCII data versions of the US-NR1 AmeriFlux data and information related to each data release (a replica of what can be found at http://urquell.colorado.edu/data_ameriflux/), (iv) a web calendar (in HTML format) documenting activity at the site (a replica of http://urquell.colorado.edu/calendar/), (v) photos (over 15,000) and video taken at the site between years 2001 and present day (2020), and (vi) several auxiliary datasets, primary related to trees near the site, soil moisture and soil temperature, and subcanopy radiation data. The data package is setup so that the web calendar, photos, and electronic logbook can be easily accessed on a local computer using a web browser. The provided data files are in either netCDF, CSV, ASCII, or MATLAB format. To obtain a better understanding about the archive, please start by reading the PDF: README_ESS_DIVE_USNR1_readme_first.pdf.

54 ENVIRONMENTAL SCIENCES↗

Videos of Chemically Activated Frontal Polymerization of Thermosets

Video recordings of the frontal ring-opening metathesis polymerization (FROMP) of thermoset polymer specimens in 10mm glass test tubes were collected in order to measure the front speed resulting from varied front initiation methods. Maximum front temperature was also measured. Polymerization resins comprised of dicyclopentadiene (DCPD) with 5 wt% 5-ethylidene-2-norbornene (ENB) added, grubbs’ second generation catalyst, and tributyl phosphite (TBP) inhibitor (8000:1:1 monomer:initiator:inhibitor ratio). Polymerization fronts were triggered using concentrated solutions of grubbs’ third generation catalyst in various solvents.

Cramblitt, Anna C.↗

Next-Generation Video Ice Particle Sampler Field Campaign Report

The next-generation video ice particle sampler (ngVIPS) was operated on the U.S. Department of Energy (DOE) Atmospheric Radiation Measurement (ARM) user facility’s tethered balloon system (TBS) during the IFFExO (Ice Fog Field Experiment at Oliktok Point) project in November, 2020. The purpose of the project was to study ice fog microphysics and the environmental conditions during ice fog to enable better predictions during low-visibility conditions. This project was conducted at the site of the third ARM Mobile Facility (AMF3) at Oliktok Point, Alaska. Among a suite of other instruments, the ngVIPS was operated to observe atmospheric ice particles.

47 OTHER INSTRUMENTATION↗

Short video provides broad overview of the Lab’s 80-year history

In celebration of the Lab’s 80th anniversary this month, join National Security Research Center Senior Historian, Alan Carr (NSRCMS), as he provides a six-and-a-half-minute overview of the Lab’s history. The video, co-produced by Brian Clayton (CEA-MP) and Howard Coe (CEA-MP), covers three phases of the Lab’s evolution, from a nuclear weapons laboratory, then a nuclear science laboratory, to its manifestation today as a multidisciplinary laboratory.

99 GENERAL AND MISCELLANEOUS↗