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At least 379 records · Page 21

Application of ERTS-1-data to the protection and management of New Jersey's coastal environment

The author has identified the following significant results. New Jersey's planned, regionalized network of sewage disposal facilities has been plotted on an ERTS-1 mosaic and circulation parameters for each of the planned outfall locations have been analyzed using the ERTS-1 imagery and comparative aircraft photography. Work is continuing on the circulation and dispersion of barge-dumped wastes in the New York Bight area. One of the largest remote sensing experiments ever attempted in this country was completed on April 7, 1973 during the ERTS-1 overpass. The test area included the northern portion of New Jersey and the Raritan Bay - New York Harbor area. Three NASA aircraft, two helicopters, nine surface vessels, 40 ground team personnel, and numerous oceanographic, radiometric, and meteorological equipment were deployed in an effort to characterize the surface and near-surface circulation dynamics in this 600 square mile area, during an entire tidal cycle. The analyses of these data in concert with all previous ERTS-1 overpasses will provide information that can lead to a better and more rational use of the nearshore marine environment. The data will be utilized to plan future outfall locations, regulating offshore disposal of wastes, etc.

Yunghans, R. S.↗

Math modelling as a complement to the scientific inquiry of physiological adaptation to space flight: Fluid, endocrine and circulatory regulation

The contribution of mathematical modeling and computer simulation to the understanding of problems arising during weightless space flight is reviewed. Examples from fluid, endocrine, and circulatory regulation are provided to illustrate the utility of modeling as an adjunct to the process of scientific inquiry, especially in the development and theoretical testing of hypotheses. The models were used to examine the acute and chronic phases of space flight. Paradoxial results appear when data obtained during chronic adaptation is interpreted using theories which pertain to acute adjustments. The modeling process provides a means of developing a theoretical basis for interpreting the chronic adaptive phase of flight.

Leonard, J. I.↗

Self-Oscillating Inductive Boost Regulator

Improved voltage regulator designed for use in solar photovoltaic dc power system utilizing flywheel for energy storage. Includes self-oscillating inductive voltage-boosting circuit, which regulates withdrawal of energy from flywheel and motor/generator when output of solar cells insufficient to maintain load voltage at specified level of 150 Vdc. Features include optical coupling and rapid response.

Paulkovich, John↗

Low-Pressure Long-Term Xenon Storage for Electric Propulsion

This Phase 2 effort demonstrated an alternative Xe storage and regulation system using activated carbon (AC) as a secondary storage media (ACSFR). This regulator system is nonmechanical, simple, inexpensive, and lighter. The ACSFR system isolates the thruster from the compressed gas tank, and allows independent multiple setpoint thruster operation. The flow using an ACSFR can also be throttled by applying increments in electrical power. Primary storage of Xe by AC is not superior to compressed gas storage with regard to weight, but AC storage can provide volume reduction, lower pressures in space, and potentially in situ Xe purification. With partial fill designs, a primary AC storage vessel for Xe could also eliminate problems with two-phase storage and regulate pressure. AC could also be utilized in long-term large quantity storage of Xe serving as a compact capture site for boil-off. Several Xe delivery ACSFR protocols between 2 and 45 sccm, and 15 min to 7 hr, were tested with an average flow variance of 1.2 percent, average power requirements of 5 W, and repeatability s of about 0.4 percent. Power requirements are affected by ACSFR bed sizing and flow rate/ duration design points, and these flow variances can be reduced by optimizing PID controller parameters.

Back, Dwight D.↗

Orion ECLSS/Suit System - Ambient Pressure Integrated Suit Test

The Ambient Pressure Integrated Suit Test (APIST) phase of the integrated system testing of the Orion Vehicle Atmosphere Revitalization System (ARS) technology was conducted for the Multipurpose Crew Vehicle (MPCV) Program within the National Aeronautics and Space Administration (NASA) Exploration Systems Mission Directorate. Crew and Thermal Systems Division performed this test in the eleven-foot human-rated vacuum chamber at the NASA Johnson Space Center. This testing is the first phase of suit loop testing to demonstrate the viability of the Environmental Control and Life Support System (ECLSS) being developed for Orion. APIST is the first in a series, which will consist of testing development hardware including the Carbon dioxide and Moisture Removal Amine Swing-bed (CAMRAS) and the air revitalization loop fan with human test subjects in pressure suits at varying suit pressures. Follow-on testing, to be conducted in 2013, will utilize the CAMRAS and a development regulator with human test subjects in pressure suits at varying cabin and suit pressures. This paper will discuss the results and findings of APIST and will also discuss future testing.

Barido, Richard A.↗

Proposed Algorithm for Placement and Sizing of Generation and Storage Stations in Urban Environments

The placement of generation and storage stations (GSSs) in distribution grids has been extensively investigated. Most traditional methods are applicable to rural or homogeneous environments and do not account for external restrictions on generation placement in urban or semi-urban environments. In this article, we propose a method for generation placement considering externality constraints. New utility-scale generation in distribution grids potentially occupies footprint and interferes in areas with existing infrastructure with architectural, historical, or touristic value. Urban environments are often regulated by municipal legislation. The placement of utility-scale generation in urban landscapes is economically and physically restricted by such externalities, and existing methods for generation placement in distribution grids based on technical optimization fail to account for this important nuance. The proposed algorithm flexibly adapts to changes in government energy policies and priorities. The selection of the type of generation suitable for the power grid is left to the preference of external high-level stakeholders, such as urban planners, industry development leaders, and energy policymakers. The proposed algorithm is a unique tool for determining the placement and sizing of generation in realistic conditions in distribution grids; it is adaptable to urban externalities and sensitive to stakeholder preferences.

generation and storage station placement↗

Optimal Operation and Dispatch of Voltage Regulation Devices Considering High Penetrations of Distributed Photovoltaic Generation

Voltage regulation devices have been traditionally installed and utilized to support distribution voltages. Installations of distributed energy resources (DERs) in distribution systems are rapidly increasing, and many of these generation resources have variable and uncertain power output. These generators can significantly change the voltage profile for a feeder; therefore, in the distribution system planning stage of the optimal operation and dispatch of voltage regulation devices, possible high penetrations of DERs should be considered. In this paper, we model the IEEE 34-bus test feeder, including all essential equipment. An optimization method is adopted to determine the optimal siting and operation of the voltage regulation devices in the presence of distributed solar power generation. Finally, we verify the optimal configuration of the entire system through the optimization and simulation results.

14 SOLAR ENERGY↗

C-Myc Induced Compensated Cardiac Hypertrophy Increases Free Fatty Acid Utilization for the Citric Acid Cycle

The protooncogene C-Myc (Myc) regulates cardiac hypertrophy. Myc promotes compensated cardiac function, suggesting that the operative mechanisms differ from those leading to heart failure. Myc regulation of substrate metabolism is a reasonable target, as Myc alters metabolism in other tissues. We hypothesize that Myc-induced shifts in substrate utilization signal and promote compensated hypertrophy. We used cardiac specific Myc-inducible C57/BL6 male mice between 4-6 months old that develop hypertrophy with tamoxifen (tam). Isolated working hearts and 13Carbon (13C )-NMR were used to measure function and fractional contributions (Fc) to the citric acid cycle by using perfusate containing 13C-labeled free fatty acids, acetoacetate, lactate, unlabeled glucose and insulin. Studies were performed at pre-hypertrophy (3-days tam, 3dMyc), established hypertrophy (7-days tam, 7dMyc) or vehicle control (cont). Non-transgenic siblings (NTG) received 7-days tam or vehicle to assess drug effect. Hypertrophy was confirmed by echocardiograms and heart weights. Western blots were performed on key metabolic enzymes. Hypertrophy occurred in 7dMyc only. Cardiac function did not differ between groups. Tam alone did not affect substrate contribution in NTG. Substrate utilization was not significantly altered in 3dMyc versus cont. The free fatty acid FC was significantly greater in 7dMyc vs cont with decreased unlabeled Fc, which is predominately exogenous glucose. Free fatty acid flux to the citric acid cycle increased while lactate flux was diminished in 7dMyc compared to cont. Total protein levels of a panel of key metabolic enzymes were unchanged; however total protein O-GlcNAcylation was increased in 7dMyc. Substrate utilization changes did not precede hypertrophy; therefore they are not the primary signal for cardiac growth in this model. Free fatty acid utilization and oxidation increase at established hypertrophy. Understanding the mechanisms whereby this change maintained compensated function could provide useful information for developing metabolic therapies to treat heart failure. The molecular signaling for this metabolic change may occur through O-GlcNAcylation.

Environmental Molecular Sciences Laboratory↗

A monomeric mycobacteriophage immunity repressor utilizes two domains to recognize an asymmetric DNA sequence

Regulation of bacteriophage gene expression involves repressor proteins that bind and downregulate early lytic promoters. A large group of mycobacteriophages code for repressors that are unusual in also terminating transcription elongation at numerous binding sites (stoperators) distributed across the phage genome. Here we provide the X-ray crystal structure of a mycobacteriophage immunity repressor bound to DNA, which reveals the binding of a monomer to an asymmetric DNA sequence using two independent DNA binding domains. The structure is supported by small-angle X-ray scattering, DNA binding, molecular dynamics, and in vivo immunity assays. We propose a model for how dual DNA binding domains facilitate regulation of both transcription initiation and elongation, while enabling evolution of other superinfection immune specificities.

59 BASIC BIOLOGICAL SCIENCES↗

Solar bus regulator and battery charger for IMP's H, I, and J

Interplanetary Monitoring Probe (IMP) spacecrafts H, I, and J utilize a direct energy transfer (DET) type of power system operating from a solar array source. A shunt type of regulator prevents the bus voltage from exceeding a preset voltage level. The power system utilizes a single differential amplifier with dual outputs to control the battery charge/shunt regulator and the discharge regulator. A two-voltage level, current limited, series charger and a current sensor control battery state of charge of the silver-cadmium battery pack. Premature termination of the battery charge is prevented by a power available gate that also initiates charge current to the battery upon availability of excess power.

Paulkovich, J.↗

Lessons Learned From Public Outreach: Effective Incorporation of the Stakeholder Voice - 18447

The licensing process for any facility associated with nuclear power or aspects of the nuclear fuel cycle can be expected to encounter intense stakeholder interest, and often significant challenges. Over the last decade, staff of the Center for Nuclear Waste Regulatory Analyses (CNWRA{sup R}) at Southwest Research Institute{sup R} (SwRI{sup R}) provided comprehensive support to the U.S. Nuclear Regulatory Commission (NRC) for public outreach efforts across a wide variety of nuclear activities. Many of these projects included public involvement and consultation activities for compliance with the National Environmental Policy Act (NEPA), including scoping, public meetings, responding to public comments on draft documents, and holding small meetings with affected local, state, and tribal governments. In addition, we have supported NRC public outreach related to nuclear facility licensing activities (including the proposed repository at Yucca Mountain), rulemaking, and regulatory guidance development. This experience has offered us a broad view of the challenges faced by industry, regulators, and stakeholders. In the process, we have observed some best practices that lead to effective stakeholder communication. Communication about complex technical issues is challenging, particularly for societally controversial topics, such as civilian use of nuclear power and management of radioactive waste. Most commonly, we have observed a lack of mutual trust and respect among industry, regulators, and stakeholders that creates an atmosphere ripe for suspicion and misunderstanding. Stakeholders have, in public comments, criticized the lack of appropriate venues to raise concerns in which they feel they can get meaningful responses from the nuclear industry and regulators. Conversely, industry faces difficulty in reaching compromise with stakeholders, who sometimes misinterpret or misrepresent factual information. Within this contentious environment, regulators are constrained by their statutory framework, even as they are challenged to be more responsive, transparent, and fair to both stakeholders and industry. In spite of these obstacles, there are examples where feedback from stakeholders was heard and utilized. Because of successful collection of appropriate feedback, regulators became more adept at understanding the full range of perspectives from both industry and stakeholders, and subsequently did a better job at effectively incorporating feedback and communicating their decisions and findings in a clear and understandable way. Ultimately, the success of these examples was based on a measure of mutual respect, which was reached through effective communication and implementation of some best practices. These best practices include (i) outreach planning that is abundantly mindful of the various stakeholder points of view, site history, regional characteristics, and probable future and related actions, combined with advance identification of key stakeholders; (ii) development and distribution of information that facilitates public participation; and (iii) use of avenues by which stakeholders can provide meaningful and effective comments. Illustrative examples include (i) how concerns raised by stakeholders were heard by an applicant and resulted in a revised project proposal; (ii) use of a variety of interaction formats that incorporated input across a broad spectrum of stakeholders; (iii) utilizing stakeholder feedback received during scoping; and (iv) how a facility underestimated the impact of 'outsider' stakeholder voices that were in contrast to the known local community. By moving past the viewpoint that public outreach is a 'necessary evil' and engaging only to check a procedural box, each participant can benefit from mutually satisfactory and improved outcomes. (author)

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

Integrated Genome-Based Studies of Shewanella Ecophysiology

Integration of bioinformatics and experimental techniques was applied to mapping and characterization of the key components (pathways, enzymes, transporters, regulators) of the core metabolic machinery in Shewanella oneidensis and related species with main focus was on metabolic and regulatory pathways involved in utilization of various carbon and energy sources. Among the main accomplishments reflected in ten joint publications with other participants of Shewanella Federation are: (i) A systems-level reconstruction of carbohydrate utilization pathways in the genus of Shewanella (19 species). This analysis yielded reconstruction of 18 sugar utilization pathways including 10 novel pathway variants and prediction of > 60 novel protein families of enzymes, transporters and regulators involved in these pathways. Selected functional predictions were verified by focused biochemical and genetic experiments. Observed growth phenotypes were consistent with bioinformatic predictions providing strong validation of the technology and (ii) Global genomic reconstruction of transcriptional regulons in 16 Shewanella genomes. The inferred regulatory network includes 82 transcription factors, 8 riboswitches and 6 translational attenuators. Of those, 45 regulons were inferred directly from the genome context analysis, whereas others were propagated from previously characterized regulons in other species. Selected regulatory predictions were experimentally tested. Integration of this analysis with microarray data revealed overall consistency and provided additional layer of interactions between regulons. All the results were captured in the new database RegPrecise, which is a joint development with the LBNL team. A more detailed analysis of the individual subsystems, pathways and regulons in Shewanella spp included bioinfiormatics-based prediction and experimental characterization of: (i) N-Acetylglucosamine catabolic pathway; (ii)Lactate utilization machinery; (iii) Novel NrtR regulator of NAD biosynthesis; (iv) HexR-controlled global regulon in central metabolism. In addition to numerous specific findings contributing to basic understanding of ecophysiology and evolution of Shewanella, the key components of the integrative genomic methodology of general utility for the community were optimized, validated and disseminated.

59 BASIC BIOLOGICAL SCIENCES↗

State Approaches to Demand Reduction Induced Price Effects: Examining How Energy Efficiency Can Lower Prices for All

Effects (DRIPE) as a real, quantifiable benefit of energy efficiency and demand response programs. DRIPE is a measurement of the value of demand reductions in terms of the decrease in wholesale energy prices, resulting in lower total expenditures on electricity or natural gas across a given grid. Crucially for policymakers and consumer advocates, DRIPE savings accrue not only to the subset of customers who consume less, but to all consumers. Rate-paying customers realize DRIPE savings when price reductions across an electricity or natural gas system are passed on to all retail customers as lower rates (depending upon regulation and market structure, residual savings may be wholly or partially retained by utilities). DRIPE savings, though seemingly small in terms of percent price reductions or dollars per household, can amount to hundreds of millions of dollars per year across entire states or grids. Therefore, accurately assessing DRIPE benefits can help to ensure appropriate programs are designed and implemented for energy efficiency measures. This paper reviews the existing knowledge and experience from select U.S. states regarding DRIPE (including New York and Ohio), and the potential for expanded application of the concept of DRIPE by regulators. Policymakers and public utility commissions have a critical role to play in setting the methodology for determining DRIPE, encouraging its capture by utilities, and allocating DRIPE benefits among utilities, various groups of customers, and/or society at large. While the methodologies for estimating DRIPE benefits are still being perfected, policymakers can follow the examples of states such as Maryland and Vermont in including conservative DRIPE estimates in their resource planning.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

A Proximity Labeling Strategy Provides Insights into the Composition and Dynamics of Lipid Droplet Proteomes

Lipid droplet (LD) functions are regulated by a complement of integral and peripheral proteins that associate with the bounding LD phospholipid monolayer. Defining the composition of the LD proteome has remained a challenge due to the presence of contaminating proteins in LD-enriched buoyant fractions. To overcome this limitation, we developed a proximity labeling strategy that exploits LD-targeted APEX2 to biotinylate LD proteins in living cells. Application of this approach to two different cell types identified the vast majority of previously validated LD proteins, excluded common contaminating proteins, and revealed new LD proteins. Moreover, quantitative analysis of LD proteome dynamics uncovered a role for endoplasmic reticulum-associated degradation in controlling the composition of the LD proteome. In conclusion, these data provide an important resource for future LD studies and demonstrate the utility of proximity labeling to study the regulation of LD proteomes.

59 BASIC BIOLOGICAL SCIENCES↗

Exploring the role of biotic factors in regulating the spatial variability in land surface phenology across four temperate forest sites

Here, land surface phenology (LSP), the characterization of plant phenology with satellite data, is essential for understanding the effects of climate change on ecosystem functions. Considerable LSP variation is observed within local landscapes, and the role of biotic factors in regulating such variation remains underexplored. In this study, we selected four National Ecological Observatory Network terrestrial sites with minor topographic relief to investigate how biotic factors regulate intra-site LSP variability. We utilized plant functional type (PFT) maps, functional traits, and LSP data to assess the explanatory power of biotic factors for the start and end of season (SOS and EOS) variability. Our results indicate that PFTs alone explain only 0.8–23.4% of intra-site SOS and EOS variation, whereas including functional traits significantly improves explanatory power, with cross-validation correlations ranging from 0.50 to 0.85. While functional traits exhibited diverse effects on SOS and EOS across different sites, traits related to competitive ability and productivity were important for explaining both SOS and EOS variation at these sites. These findings reveal that plants exhibit diverse phenological responses to comparable environmental conditions, and functional traits significantly contribute to intra-site LSP variability, highlighting the importance of intrinsic biotic properties in regulating plant phenology.

60 APPLIED LIFE SCIENCES↗

A Model-Predictive Hierarchical-Control Framework for Aggregating Residential DERs to Provide Grid Regulation Services: Preprint

This paper develops a hierarchical control fram-ework to aggregate and control behind-the-meter distributed energy resources (DERs), which will be ubiquitous in future distribution systems. Even though the increasing penetration of DERs will strain the power networks in terms of voltage regul-ation and coordination issues with existing transmission-level conventional generators, the distribution-level DERs can also be utilized to help provide flexibility to the power network while providing cost savings to the DER owners. Therefore, this paper develops a model-predictive control strategy to determine the available power flexibility, and to utilize the flexibility in an aggregated form to provide grid regulation services. Numerical simulations performed on the IEEE 37-bus test system demonstrate the efficacy of the proposed approach.

14 SOLAR ENERGY↗