SEARCH · Engineering Papers

Results for “Inducer”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

SourceResearch connectionFrom yearThrough year

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 379 records · Page 21

All sources NASA NTRS DOE OSTI

NASA NTRS2016

Wake-Induced Aerodynamics on a Trailing Aircraft

NASA conducted flight tests to measure the exhaust products from alternative fuels using a DC-8 transport aircraft and a Falcon business jet. An independent analysis of the maximum vortex-induced loads on the Falcon in the DC-8 wake was conducted for pre-flight safety analysis and to define safe trail distances for the flight tests. Static and dynamic vortex-induced aerodynamic loads on the Falcon were predicted at a matrix of locations aft of the DC-8 under flight-test conditions, and the maximum loads were compared with design limit loads to assess aircraft safety. Trajectory simulations for the Falcon during close encounters with the DC-8 wake were made to study the vortex-induced loads during traverses of the DC-8 primary trailing vortex. A parametric study of flight traverses through the trailing vortex was conducted to assess Falcon flight behavior and motion characteristics.

Mendenhall, Michael R.↗

NASA NTRS2017

On the Cause of Two Prompt Shock-Induced Relativistic Electron Depletion Events

Dayside interplanetary (IP) shock-induced injections are known to be a source of highly relativistic electrons in Earth's outer radiation belt, and are possibly the only source of greater than 1 megaelectronvolt electrons in the inner belt. The associated electron energization process is well understood and modeled. Recently, relativistic electron depletion echoes have also been associated with IP shocks, but the processes driving the depletions are less well understood. In this study, we investigate in detail two shock induced greater than 1 megaelectronvolt electron depletion events observed by the Van Allen Probes, March 17, 2015 and May 24, 2013, and draw similarities to night-side substorm related enhancements and depletions. Both events exhibit shock induced enhancements on one of the Van Allen Probes and depletions on the other in greater than 1 megaelectronvolt channels, such observations have not previously been reported. The depletion of greater than 1 megaelectronvolt electrons during the March 17, 2015 event is associated with enhancements in 10 second - 100 second kiloelectronvolt electrons on the same spacecraft. The depletion is consistent with the effects of a lack of seed electrons at larger radial distances combined with inward motion due to asymmetric compression by the shock impact. The immediate enhancements and depletions of 75 kiloelectronvolt - 2.6 greater than 1 megaelectronvolt electrons are explained by the local phase space density radial profile. Observations of electron flux dynamics during the May 24, 2013 event can also be explained by a lack of a seed population at larger radial distances, supported by butterfly distributions observed during the event. The electron's inward radial motion can be attributed to the inward propagating impulse also associated with the greater than 1 megaelectronvolt electron enhancements observed on the complementary probe, rather than global asymmetric compression. This causal mechanism has parallels to substorm related depletions. Alternatively, evidence is provided to attribute the sudden depletions to losses due to a sudden but brief inward motion of the magnetopause.

Schiller, Quintin↗

NASA NTRS2019

Quantifying Water Storage Change and Land Subsidence Induced by Reservoir Impoundment Using GRACE, Landsat, and GPS Data

The construction of hydropower dams is a common strategy to support a country's increasing need for electricity and river water management for industry and agriculture. Although the hydrological and geophysical impacts of water relocation are usually assessed prior to impoundment, their accuracy is generally limited due to the lack of in situ observations, especially in a remote area. This study presents a workflow to quantify the terrestrial water storage change (TWS) and land subsidence induced by a reservoir's water impoundment using multiple satellite observations (GRACE, Landsat), land surface models (CABLE, GLDAS, NCEP, ECMWF), and GPS data. The study site is the Bakun Dam, located in Sarawak, Malaysia, which is the largest hydropower dam in Southeast Asia. Commencing operation in late 2010, the dam induced a change of water mass and lake surface area that was clearly observed by GRACE and Landsat observations, respectively. During the 17-month impounding period (from August 2010 to December 2011), GRACE observed a dramatic increase of approximately 200 mm equivalent water height, while Landsat detected an increased lake extent of around 600 km2. In this paper, a forward model is developed to determine the increased water surface level corresponding to GRACE observations, estimated to be about 120 m. In contrast to GRACE, the TWS derived from land surface models cannot capture the increased TWS, due to the lack of reservoir routing algorithms in the models. In addition, the land subsidence was calculated using the disk load model constructed based on the GRACE-derived lake level and Landsat-derived lake extent; the result is validated with the GPS data from BIN1 station, located at the western coast of Borneo. The commencement stage of the Bakun Dam induces the large-scale land subsidence, which causes the GPS-BIN1 station to subside by ~9 mm, and move toward the Bakun Lake by ~4 mm. Computation of the surface displacements directly from GRACE spherical harmonic coefficient data fails to capture the subsidence feature, mainly due to the truncation error. Overall, this study demonstrates that evaluating GRACE in conjunction with Landsat, LSMs, and GPS data allows the exploitation of the gravity signal at a much smaller spatial scale than its intrinsic resolution. Benefiting from global coverage, the newly developed satellite-based algorithm is a valuable tool for assessing the impacts of reservoir operation on hydrological and geophysical changes from local to regional scales.

Natthachet Tangdamrongsub↗

NASA NTRS2019

Modeled Microgravity Induces Neutrophil Extracellular Trap (NET)osis Formation and Reduced Phagocytosis of Polymorphonuclear Neutrophils

Spaceflight can dysregulate immunity, by way of increasing granulocytes numbers with impaired function. Polymorphonuclear neutrophils (PMN) are granulocytes that are first responders to infection or injury, and consist of the largest pool of immune cells in humans. PMNs function during innate immunity, through phagocytosis and promotion of inflammation, via the release of reactive oxygen species (ROS) mediators and granule-containing enzymes, such as myeloperoxidase (MPO) and NADPH oxidase-2 (NOX-2). In addition, neutrophil extracellular trap (NET) formation is another mechanism of PMN surveillance that works independently of engulfment phagocytosis, and is a last resort function that can induce NETosis or PMN-specific cell death. Previous studies in our lab have identified increased mature neutrophils, ROS and MPO production, and reduced phagocytosis in granulocytes in simulated microgravity (sug) models of hindlimb unloading (HU) in adult mice and leukocytes cultured in high-aspect rotating wall vessels (HARV-RWV). Since sug impaired phagocytosis, but improved enzymatic mediator production of MPO and redox molecules, we sought to address the third known function of PMNs, NETosis. For this, PMNs were culture in the presence or absence of the anti-oxidant N-acetyl cysteine (NAC), which rescued impaired phagocytosis that was present in sug without NAC treatment. Further, NETosis was induced in sug that was no different in the presence of NAC, suggesting NAC targets independent functions of PMNs under sug. Collectively, these results suggest modeled microgravity induced NETosis, which opens a new avenue for spaceflight studies in immune dysfunction.

Paul, Amber M.↗

NASA NTRS2019

Quantifying Water Storage Change and Land Subsidence Induced by Reservoir Impoundment Using GRACE, Landsat, and GPS Data

The construction of hydropower dams is a common strategy to support a country's increasing need for electricityand river water management for industry and agriculture. Although the hydrological and geophysical impacts ofwater relocation are usually assessed prior to impoundment, their accuracy is generally limited due to the lack ofin situ observations, especially in a remote area. This study presents a workflow to quantify the terrestrial waterstorage change (TWS) and land subsidence induced by a reservoir's water impoundment using multiple satelliteobservations (GRACE, Landsat), land surface models (CABLE, GLDAS, NCEP, ECMWF), and GPS data. The studysite is the Bakun Dam, located in Sarawak, Malaysia, which is the largest hydropower dam in Southeast Asia.Commencing operation in late 2010, the dam induced a change of water mass and lake surface area that wasclearly observed by GRACE and Landsat observations, respectively. During the 17-month impounding period(from August 2010 to December 2011), GRACE observed a dramatic increase of approximately 200mmequivalent water height, while Landsat detected an increased lake extent of around 600 km2. In this paper, aforward model is developed to determine the increased water surface level corresponding to GRACE observations,estimated to be about 120 m. In contrast to GRACE, the TWS derived from land surface models cannotcapture the increased TWS, due to the lack of reservoir routing algorithms in the models. In addition, the landsubsidence was calculated using the disk load model constructed based on the GRACE-derived lake level andLandsat-derived lake extent; the result is validated with the GPS data from BIN1 station, located at the westerncoast of Borneo. The commencement stage of the Bakun Dam induces the large-scale land subsidence, whichcauses the GPS-BIN1 station to subside by ~9 mm, and move toward the Bakun Lake by ~4 mm. Computation ofthe surface displacements directly from GRACE spherical harmonic coefficient data fails to capture the subsidencefeature, mainly due to the truncation error. Overall, this study demonstrates that evaluating GRACE inconjunction with Landsat, LSMs, and GPS data allows the exploitation of the gravity signal at a much smallerspatial scale than its intrinsic resolution. Benefiting from global coverage, the newly developed satellite-basedalgorithm is a valuable tool for assessing the impacts of reservoir operation on hydrological and geophysicalchanges from local to regional scales.

Tangdamrongsub, Natthachet↗

NASA NTRS2020

A Bi-Exponential Repair Algorithm for Radiation-Induced Double-Strand Breaks: Application to Chromosome Aberrations

Chromosome aberrations (CAs) are one of the effects of radiation exposure and are used as a biomarker. A new simulation program, named RITCARD (Radiation induced tracks, chromosome aberrations, repair, and damage) was developed to simulate radiation-induced CA. RITCARD is used with the program RITRACKS (Relativistic Ion Tracks), which simulates the radiation tracks. The restitution kinetics algorithm presented here is a significant improvement over the one used in the first version. Simulations of radiation-induced CA were performed for several ion types and mixed irradiation fields. These simulations will be useful to help interpreting experiments of galactic cosmic rays (GCR) simulator.

Plante, Ianik↗

NASA NTRSDate not supplied

Dose, LET, time and strain dependence of radiation-induced 53BP1 foci in 15 mouse strains ex vivo and associations to in vivo radiation susceptibility

We present a comparative analysis on the repair of radiation-induced DNA damage ex vivo in 15 strains of mice, including 5 inbred reference strains and 10 collaborative-cross strains, of both sexes. Non-immortalized primary skin fibroblasts derived from 76 mice were subjected to both low- and high-LET radiation (0.1, 1 and 4 Gy of X rays; 1.1 and 3 particles/100μm2 of 350 MeV/n 40Ar and 600 MeV/n 56Fe). Automated image quantification of 53BP1 radiation-induced foci (RIF) during the first 4-48 h post-irradiation was performed as a function of dose and LET. Similarly to what we had previously reported for immortalized human cell lines [1], we observed a saturation of RIF number with dose at 4h post-irradiation, with more RIF/Gy for lower LET (X rays and 40Ar) compared to 56Fe. However at later time points (24h and above), the trend was inverted with more RIF/Gy for higher LET. Our data suggest that multiple DSBs cluster into RIF: as the linear density of DSBs increases with LET, so does the probability of having more DSBs per RIF, which makes it more difficult for cells to fully resolve high-LET-induced RIF, explaining the hypersensitivity to high-LET radiation despite a low number of RIF. Taking into account the amount of clustering at a given dose and LET, but also the kinetics of DNA damage repair, we introduced a novel mathematical formalism to evaluate the number of remaining RIF over time. We showed that the newly introduced kinetic metrics can be used as surrogate biomarkers for in vivo radiation toxicity, with potential applications in radiotherapy and human space exploration. In particular, we observed an association between the repairable fraction of RIF measured in vitro and survival levels of immune cells collected from irradiated mice. Moreover, the speed of DNA damage repair correlated with spontaneous cancer incidence data collected from the Mouse Tumor Biology database, suggesting a relationship between the efficiency of DSB repair after irradiation and cancer risk. In addition to the efficacy of repair and persistent RIF levels, even the amount of spontaneous foci without irradiation was shown to be strain dependent, indicating that these phenotypes are at least partially driven by genetics, and supporting their potential as indicators of individual radiation sensitivity. [1] Neumaier, T., et al., PNAS, 2012 (8) 109:443

Radiation, DNA damage, repair kinetics↗

NASA NTRSDate not supplied

Considering Cell Proliferation to Optimize Detection of Radiation-induced 53BP1+ Foci in 15 Mouse Strains ex vivo

Due to high metabolic activity, proliferating cells continuously generate free radicals, which induce DNA double strand breaks (DSB). Fluorescently tagged nuclear foci of DNA repair protein 53 binding protein-1(53BP1) are used as a standard metric for measuring DSB formation at baseline and in response to environmental insults such as radiation. Here we demonstrate that the background level of spontaneous 53BP1+ foci formation can be modeled mathematically as a function of cell confluence, which is a metric of proliferation rate. This model was validated using spontaneous 53BP1+ foci data from 68 cultures of primary skin fibroblasts derived from 15 different strains of mice, showing a ~10 fold decrease from low to full confluence that is independent of mouse strain. We developed an online open access tool to correct for the impact of cell confluence on the detection of radiation-induced 53BP1+ foci (RIF). This tool provides guidelines for the number of cells required to reach statistical significance for the detection of excess foci induced by low doses of ionizing radiation as a function of confluence and time post-irradiation. We hope that this quantification tool will help the radiation biology community in the design of future experiments that utilize 53BP1+ foci-based quantification of radiation responses in vitro. Our “tool for enhanced results of RIF in cells” (terRIFic) can be found at: https://radbiolab.shinyapps.io/terrific/

Radiation, DNA damage, confluence↗

NASA NTRS2022

Insights from the Topographic Characteristics of a Large Global Catalog of Rainfall-Induced Landslide Event Inventories

Landslides are a key hazard in high-relief areas around the world and pose a risk to population and infrastructure. It is important to understand where landslides are likely to occur in the landscape to inform local analyses of exposure and potential impacts. Large triggering events such as earthquakes or major rain storms often cause hundreds or thousands of landslides, and mapping the landslide populations generated by these events can provide extensive datasets of landslide locations. Previous work has explored the characteristic locations of landslides triggered by seismic shaking, but rainfall induced landslides are likely to occur in different parts of a given landscape when compared to seismically induced failures. Here we show measurements of a range of topographic parameters associated with rainfall-induced landslides inventories, including a number of previously unpublished inventories which we also present here. We find that average upstream angle and compound topographic index are strong predictors of landslide scar location, while local relief and topographic position index provide a stronger sense of where landslide material may end up (and thus where hazard may be highest). By providing a large compilation of inventory data for open use by the landslide community, we suggest that this work could be useful for other regional and global landslide modelling studies and local calibration of landslide susceptibility assessment, as well as hazard mitigation studies.

Robert Emberson↗

NASA NTRS2003

Non-targeted and delayed effects of exposure to ionizing radiation: II. Radiation-induced genomic instability and bystander effects in vivo, clastogenic factors and transgenerational effects

The goal of this review is to summarize the evidence for non-targeted and delayed effects of exposure to ionizing radiation in vivo. Currently, human health risks associated with radiation exposures are based primarily on the assumption that the detrimental effects of radiation occur in irradiated cells. Over the years a number of non-targeted effects of radiation exposure in vivo have been described that challenge this concept. These include radiation-induced genomic instability, bystander effects, clastogenic factors produced in plasma from irradiated individuals that can cause chromosomal damage when cultured with nonirradiated cells, and transgenerational effects of parental irradiation that can manifest in the progeny. These effects pose new challenges to evaluating the risk(s) associated with radiation exposure and understanding radiation-induced carcinogenesis.

Review, Tutorial↗

NASA NTRS1998

The potential influence of radiation-induced microenvironments in neoplastic progression

Ionizing radiation is a complete carcinogen, able both to initiate and promote neoplastic progression and is a known carcinogen of human and murine mammary gland. Tissue response to radiation is a composite of genetic damage, cell death and induction of new gene expression patterns. Although DNA damage is believed to initiate carcinogenesis, the contribution of these other aspects of radiation response are beginning to be explored. Our studies demonstrate that radiation elicits rapid and persistent global alterations in the mammary gland microenvironment. We postulate that radiation-induced microenvironments may affect epithelial cells neoplastic transformation by altering their number or susceptibility. Alternatively, radiation induced microenvironments may exert a selective force on initiated cells and/or be conducive to progression. A key impetus for these studies is the possibility that blocking these events could be a strategy to interrupt neoplastic progression.

Review, Academic↗

NASA NTRS2013

Motion-Based Piloted Simulation Evaluation of a Control Allocation Technique to Recover from Pilot Induced Oscillations

This paper describes the maturation of a control allocation technique designed to assist pilots in the recovery from pilot induced oscillations (PIOs). The Control Allocation technique to recover from Pilot Induced Oscillations (CAPIO) is designed to enable next generation high efficiency aircraft designs. Energy efficient next generation aircraft require feedback control strategies that will enable lowering the actuator rate limit requirements for optimal airframe design. One of the common issues flying with actuator rate limits is PIOs caused by the phase lag between the pilot inputs and control surface response. CAPIO utilizes real-time optimization for control allocation to eliminate phase lag in the system caused by control surface rate limiting. System impacts of the control allocator were assessed through a piloted simulation evaluation of a non-linear aircraft simulation in the NASA Ames Vertical Motion Simulator. Results indicate that CAPIO helps reduce oscillatory behavior, including the severity and duration of PIOs, introduced by control surface rate limiting.

pilot induced oscillation↗

NASA NTRS2013

Diverse Electron-Induced Optical Emissions from Space Observatory Materials at Low Temperatures

Electron irradiation experiments have investigated the diverse electron-induced optical and electrical signatures observed in ground-based tests of various space observatory materials at low temperature. Three types of light emission were observed: (i); long-duration cathodoluminescence which persisted as long as the electron beam was on (ii) short-duration (<1 s) arcing, resulting from electrostatic discharge; and (iii) intermediate-duration (~100 s) glow-termed "flares". We discuss how the electron currents and arcing-as well as light emission absolute intensity and frequency-depend on electron beam energy, power, and flux and the temperature and thickness of different bulk (polyimides, epoxy resins, and silica glasses) and composite dielectric materials (disordered SiO2 thin films, carbon- and fiberglass-epoxy composites, and macroscopically-conductive carbon-loaded polyimides). We conclude that electron-induced optical emissions resulting from interactions between observatory materials and the space environment electron flux can, in specific circumstances, make significant contributions to the stray light background that could possibly adversely affect the performance of space-based observatories.

long-duration cathodoluminescence↗

NASA NTRS2014

The Development and Deployment of a Ground-Based, Laser-Induced Fluorescence Instrument for the In Situ Detection of Iodine Monoxide Radicals

High abundances of iodine monoxide (IO) are known to exist and to participate in local photochemistry of the marine boundary layer. Of particular interest are the roles IO plays in the formation of new particles in coastal marine environments and in depletion episodes of ozone and mercury in the Arctic polar spring. This paper describes a ground-based instrument that measures IO at mixing ratios less than one part in 1012. The IO radical is measured by detecting laser-induced fluorescence at wavelengths longer that 500 nm. Tunable visible light is used to pump the A23/2 (v = 2) ← X23/2 (v = 0) transition of IO near 445 nm. The laser light is produced by a solid-state, Nd:YAG-pumped Ti:Sapphire laser at 5 kHz repetition rate. The laser-induced fluorescence instrument performs reliably with very high signal-to-noise ratios (>10) achieved in short integration times (<1 min). The observations from a validation deployment to the Shoals Marine Lab on Appledore Island, ME are presented and are broadly consistent with in situ observations from European Coastal Sites. Mixing ratios ranged from the instrumental detection limit (<1 pptv) to 10 pptv. These data represent the first in situ point measurements of IO in North America.

marine boundary layer↗

NASA NTRS2014

Role of Oxidative Damage in Radiation-Induced Bone Loss

During prolonged spaceflight, astronauts are exposed to both microgravity and space radiation, and are at risk for increased skeletal fragility due to bone loss. Evidence from rodent experiments demonstrates that both microgravity and ionizing radiation can cause bone loss due to increased bone-resorbing osteoclasts and decreased bone-forming osteoblasts, although the underlying molecular mechanisms for these changes are not fully understood. We hypothesized that excess reactive oxidative species (ROS), produced by conditions that simulate spaceflight, alter the tight balance between osteoclast and osteoblast activities, leading to accelerated skeletal remodeling and culminating in bone loss. To test this, we used the MCAT mouse model; these transgenic mice over-express the human catalase gene targeted to mitochondria, the major organelle contributing free radicals. Catalase is an anti-oxidant that converts reactive species, hydrogen peroxide into water and oxygen. This animal model was selected as it displays extended lifespan, reduced cardiovascular disease and reduced central nervous system radio-sensitivity, consistent with elevated anti-oxidant activity conferred by the transgene. We reasoned that mice overexpressing catalase in mitochondria of osteoblast and osteoclast lineage cells would be protected from the bone loss caused by simulated spaceflight. Over-expression of human catalase localized to mitochondria caused various skeletal phenotypic changes compared to WT mice; this includes greater bone length, decreased cortical bone area and moment of inertia, and indications of altered microarchitecture. These findings indicate mitochondrial ROS are important for normal bone-remodeling and skeletal integrity. Catalase over-expression did not fully protect skeletal tissue from structural decrements caused by simulated spaceflight; however there was significant protection in terms of cellular oxidative damage (MDA levels) to the skeletal tissue. Furthermore, we used an array of countermeasures (Antioxidant diets and injections) to prevent the radiation-induced bone loss, although these did not prevent bone loss, analysis is ongoing to determine if these countermeasure protected radiation-induced damage to other tissues.

oxidative damage↗

NASA NTRS2016

Maven Observations of Electron-Induced Whistler Mode Waves in the Martian Magnetosphere

We report on narrowband electromagnetic waves at frequencies between the local electron cyclotron and lower hybrid frequencies observed by the Mars Atmosphere and Volatile EvolutioN (MAVEN) spacecraft in the Martian induced magnetosphere. The peaked electric field wave spectra below the electron cyclotron frequency were first observed by Phobos-2 in the Martian magnetosphere, but the lack of magnetic field wave data prevented definitive identification of the wave mode and their generation mechanisms remain unclear. Analysis of electric and magnetic field wave spectra obtained by MAVEN demonstrates that the observed narrowband waves have properties consistent with the whistler mode. Linear growth rates computed from the measured electron velocity distributions suggest that these whistler mode waves can be generated by cyclotron resonance with anisotropic electrons. Large electron anisotropy in the Martian magnetosphere is caused by absorption of parallel electrons by the collisional atmosphere. The narrowband whistler mode waves and anisotropic electrons are observed on both open and closed field lines and have similar spatial distributions in MSO and planetary coordinates. Some of the waves on closed field lines exhibit complex frequency-time structures such as discrete elements of rising tones and two bands above and below half the electron cyclotron frequency. These MAVEN observations indicate that whistler mode waves driven by anisotropic electrons, which are commonly observed in intrinsic magnetospheres and at unmagnetized airless bodies, are also present at Mars. The wave-induced electron precipitation into the Martian atmosphere should be evaluated in future studies.

martian↗

NASA NTRS2018

Coalescence of DNA Double Strand Breaks Induced by Galactic Cosmic Radiation is Modulated by Genetics in 15 Inbred Strains of Mice

In this manuscript we address the challenges associated with the ability to predict radiation sensitivity associated with exposure to either cosmic radiation or X-rays in a population study, by monitoring DNA damage sensing protein 53BP1 forming small nuclear radiation-induced foci (RIF) as a surrogate biomarker of DNA double strand breaks (DSB). 76 primary skin fibroblasts were isolated from 10 collaborative cross strains and five reference inbred mice (C57Bl/6, BALB/CByJ, B6C3, C3H and CBA/CaJ) and exposed to three different charged nuclei of increasing LET (350 MeV/n Si, 350 MeV/n Ar and 600 MeV/n Fe) and X-ray. Our data brings strong evidence against the classic "contact-first" model where DSBs are assumed to be immobile and repaired at the lesion site. In contrast, our model suggests nearby DSBs move into single repair unit characterized by large RIF before the repair machinery kicks in. Such model has the advantage of being much more efficient molecularly but is poorly suited to deal with cosmic radiation, where energy is concentrated along the particle trajectory, inducing a large density of DSBs along each particle track. In accordance with this model, RIF quantification after X-ray exposition showed a saturated dose response for early time points post-irradiation for all strains. Similarly, the high-LET response showed that RIF number matched the number of track per cell, not the number of expected DSB per cell (1). At the temporal level, we noted that the percentage of unrepaired high-LET tracks over a 48 hour time-course increased with LET, confirming that the DNA repair process becomes more difficult as more DSB coalesce into single RIF. There was also good agreement between persistent RIF levels measured in-vitro in the primary skin cultures and survival levels of T-cells and B-cells collected in blood samples from 10 CC strains 24 hours after 0.1 Gy whole-body dose of X-ray. This suggests that persistent RIF 24 hour post-IR is a good surrogate in-vitro biomarker for in-vivo radiation toxicity. Finally, at the genomic level, large differences in repair rates between strains for high-LET allowed us to identify suggestive genetic loci associated with radiation sensitivity. Interestingly, the two highest LETs provided the most strain variation with a common locus on Chromosome 10 highly enriched for DNA repair associated genes we discussed in detail.

Radiation-Induced Foci↗

NASA NTRS2019

Diurnal and Seasonal Solar Induced Chlorophyll Fluorescence and Photosynthesis in a Boreal Scots Pine Canopy

Solar induced chlorophyll fluorescence has been shown to be increasingly an useful proxy for the estimation of gross primary productivity (GPP), at a range of spatial scales. Here, we explore the seasonality in a continuous time series of canopy solar induced fluorescence (hereafter SiF) and its relation to canopy gross primary production (GPP), canopy light use efficiency (LUE), and direct estimates of leaf level photochemical efficiency in an evergreen canopy. SiF was calculated using infilling in two bands from the incoming and reflected radiance using a pair of Ocean Optics USB2000+ spectrometers operated in a dual field of view mode, sampling at a 30 min time step using custom written automated software, from early spring through until autumn in 2011. The optical system was mounted on a tower of 18 m height adjacent to an eddy covariance system, to observe a boreal forest ecosystem dominated by Scots pine. (Pinus sylvestris) A Walz MONITORING-PAM, multi fluorimeter system, was simultaneously mounted within the canopy adjacent to the footprint sampled by the optical system. Following correction of the SiF data for O2 and structural effects, SiF, SiF yield, LUE, the photochemicsl reflectance index (PRI), and the normalized difference vegetation index (NDVI) exhibited a seasonal pattern that followed GPP sampled by the eddy covariance system. Due to the complexities of solar azimuth and zenith angle (SZA) over the season on the SiF signal, correlations between SiF, SiF yield, GPP, and LUE were assessed on SZA <50° and under strictly clear sky conditions. Correlations found, even under these screened scenarios, resulted around ~r2 = 0.3. The diurnal responses of SiF, SiF yield, PAM estimates of effective quantum yield (ΔF/Fm′), and meteorological parameters demonstrated some agreement over the diurnal cycle. The challenges inherent in SiF retrievals in boreal evergreen ecosystems are discussed.

photosynthetic↗