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NASA NTRSDate not supplied

BioNutrients-2: Improvements to the BioNutrients-1 Nutrient Production System

The BioNutrients (BN) project aims to develop an on-demand nutrient production system capable of rapid and safe delivery of multiple nutrients in a single-use production pack for long-duration missions. The general concept of this system will be to develop an edible engineered microorganism capable of surviving during long-term storage, revival and growth of that microorganism for product production, and delivery of precise quantities of nutrients for human consumption. BN-1 is a five-year flight project currently on the International Space Station (ISS). It is testing the long-term storage of various microorganisms for the biomanufacturing of space relevant compounds and to validate the performance of the first generation (Gen-1) of production packs. The BN-2 project is a continuation of the BN-1 flight project and intends to expand the projects overall scope by introducing novel products, broadening the range of microbial food sources, and improving previously designed hardware from the BN-1 project. We are in the process of optimizing our platform biomanufacturing technology for in situ production of yogurt and kefir on the ISS. BN-2 will test the efficacy of this technology for future delivery of probiotic organisms which can be engineered to produce additional nutritional supplements (consumption by the crew is not planned in this project). Furthermore, to improve the feasibility of this technology for mission use we have transitioned from a hard-shell pack to a Gen-2, fluorinated ethylene propylene (FEP) bag. The single-use FEP bioreactor will facilitate growth of known organisms and ensure a contaminant free environment, while reducing mass and volume. These bioreactors will be employed for production of additional space relevant products in the BN-2 project. On-orbit testing of Gen-2 production packs will be necessary for future iterations, in order to optimize bag design and product production for eventual flight use by the crew.

BioNutrients↗
NASA NTRS2021

Maps of Magnetic Field Strength in the OMC-1 Using HAWC+FIR Polarimetric Data

Far-infrared dust polarimetry enables the study of interstellar magnetic fields via tracing of the polarized emission from dust grains that are partially aligned with the direction of the field. The advent of high-quality polarimetric data has permitted the use of statistical methods to extract both the direction and magnitude of the magnetic field. In this work, the Davis–Chandrasekhar–Fermi technique is used to make maps of the plane-of-sky (POS) component of the magnetic field in the Orion Molecular Cloud (OMC-1) by combining polarization maps at 53, 89, 154 and 214 μm from HAWC+/SOFIA with maps of density and velocity dispersion. In addition, maps of the local dispersion of polarization angles are used in conjunction with Zeeman measurements to estimate a map of the strength of the line-of-sight (LOS) component of the field. Combining these maps, information about the threedimensional magnetic field configuration (integrated along the LOS) is inferred over the OMC-1 region. POS magnetic field strengths of up to 2 mG are observed near the BN/KL object, while the OMC-1 bar shows strengths of up to a few hundred μG. These estimates of the magnetic field components are used to produce maps of the mass-to-magnetic-flux ratio (M/Φ)—a metric for probing the conditions for star formation in molecular clouds— and determine regions of sub- and supercriticality in OMC-1. Such maps can provide invaluable input and comparison to MHD simulations of star formation processes in filamentary structures of molecular clouds. Unified Astronomy Thesaurus concepts: Molecular clouds (1072); Giant molecular clouds (653); Interstellar magnetic fields (845); Far infrared astronomy (529)

Jordan A Guerra↗
NASA NTRS2020

Origins of X-ray Line Emissions in Circinus X-1 at Very Low X-ray Flux

Accretion conditions and morphologies of X-ray transients containing neutron stars are still poorly understood. Circinus X-1 is an enigmatic case where we observe X-ray flux changes covering four orders of magnitude. We observed Circinus X-1 several times at its very lowest X-ray flux using the high-energy transmission grating spectrometer on board the Chandra X-ray Observatory. At a flux of 1.8 ´ 10-11 erg cm−2 s−1 we observed a single 1.6 keV blackbody spectrum. The observed continuum luminosity of 1035 erg s−1 is about two orders of magnitude too low to explain the observed photoionized luminosity, suggesting a much more complex structure of the X-ray source that is partially or entirely obscured, as had been previously suggested. This affects most emissions from the accretion disk, including previously observed accretion-disk coronal line emissions. Instead, the strongest observed photoionized lines are blueshifted by about ∼400 km s−1, and we suggest that they originate in the ionized wind of a B5Ia supergiant companion, supporting a previous identification. The neutron star in Cir X-1 is very young and should have a high magnetic field. At the observed luminosity, the emission radius of the blackbody is small enough to be associated with the accretion hot spot as the X-ray-emitting region. The small emission radius then points to a field strength below 1012 G, which would be consistent with the observation of occasional type I X-ray bursts at high magnetic fields. We discuss Cir X-1 in the context of being a high-mass X-ray binary, with some emphasis on a possible Be-star X-ray binary nature.

N S Schulz↗
NASA NTRS2021

Magma Ocean Evolution of the TRAPPIST-1 Planets

Recent observations of the potentially habitable planets TRAPPIST-1 e, f, and g suggest that they possess large water mass fractions of possibly several tens of wt% of water, even though the host star’s activity should drive rapid atmospheric escape. These processes can photolyze water, generating free oxygen and possibly desiccating the planet. After the planets formed, their mantles were likely completely molten with volatiles dissolving and exsolving from the melt. In order to understand these planets and prepare for future observations, the magma ocean phase of these worlds must be understood. To simulate these planets, we have combined existing models of stellar evolution, atmospheric escape, tidal heating, radiogenic heating, magma ocean cooling, planetary radiation, and water-oxygen-iron geochemistry. We present Magm Oc, a versatile magma ocean evolution model, validated against the rocky Super-Earth GJ 1132b and early Earth. We simulate the coupled magma ocean-atmospheric evolution of TRAPPIST-1 e, f, and g for a range of tidal and radiogenic heating rates, as well as initial water contents between 1 and 100 Earth oceans. We also reanalyze the structures of these planets and find they have water mass fractions of 0–0.23, 0.01–0.21, and 0.11–0.24 for planets e, f, and g, respectively. Our model does not make a strong prediction about the water and oxygen content of the atmosphere of TRAPPIST-1 e at the time of mantle solidification. In contrast, the model predicts that TRAPPIST-1 f and g would have a thick steam atmosphere with a small amount of oxygen at that stage. For all planets that we investigated, we find that only 3 ́5% of the initial water will be locked in the mantle after the magma ocean solidified.

Exoplanets↗
NASA NTRS2022

SORCE and TSIS-1 SIM Comparison: Absolute Irradiance Scale Reconciliation

The Solar Radiation and Climate Experiment (SORCE) and Total and Spectral Irradiance Sensor (TSIS-1) conducted an intercomparison for the two Spectral Irradiance Monitors (SIM) spanning 704 days from 23 March 2018 to 25 February 2020 and permitted 554 time-matched pairs of observations. This comparison was conducted during the extremely quiescent Solar Cycle 24 minimum, so all observed differences and drifts between the two sensors are instrumental in nature. The TSIS-1 SIM benefitted from advanced calibration capabilities based on SI standards that were not available during the preflight calibration time period of SORCE. For this reason, a revision of the SORCE SIM absolute scale is appropriate. As expected, wavelength dependent differences in absolute agreement are a function of detector sensitivity and local changes in spectral slope. At the time of the comparison SORCE SIM has been on-orbit for 17 years while TSIS-1 observations commenced immediately after a 100-day outgassing and commissioning period. Peak-to-peak absolute scale differences are about 12% with a mean fractional difference of 0.7% ± 2.9%. The greatest scale differences occur at the change-over between the UV and visible photodiodes in the 310 nm region, and a systematic disagreement is present in the 850–1,600 nm range. A multiplicative scale correction factor has been developed to reconcile the TSIS-1 and SORCE difference with a wavelength dependent error on the mean typically less than 0.01% derived from every matched pair of observations.

Jerald Harder↗
NASA NTRSDate not supplied

Developing a Genetic Variant Calling Pipeline for Quantifying the Complex Mutagenic Load Accumulated in BioNutrients-1 Production Pack Samples

Microorganisms hold great promise for on demand production of labile nutrients and pharmaceuticals as well recycling and in situ resource utilization. The utilization of microorganisms for such tasks on space missions is hindered by the limited data on how microbes respond to spaceflight. For example, the genetic stability of microorganisms, and the genomic engineered traits added to deliver desired functions, over long-term storage in the spacecraft environment is poorly understood. The BioNutrients-1 (BN-1) mission conducted a 5-year study of desiccated storage in Low Earth Orbit (LEO) to evaluate the suitability of eight synthetic biology chassis organisms for long-duration space missions. We are employing high-depth, whole genome sequencing (WGS) to determine the mutagenic load that accumulated during long-term storage. Mutation analysis pipelines are well established for homogenous culture grown from a single colony, but the mutational landscape of the BN-1 samples present a unique analysis challenge, as every cell in the BN-1 samples had a unique genetic journey of DNA damage and repair. Consequently, sequence variants are expected at low allele frequency within samples. To address this genetic complexity, we apply two distinct computational approaches to identify mutations in pre-existing WGS data collected from populations of Chlamydomonas reinhardtii that were exposed to UV mutagenesis and growth in LEO. For reference genome free mutation detection, we utilized DiscoSNP++, which is a de Bruijn graph approach. For reference genome-based mutation detection we utilize GATK for Microbes, which is a Bayesian probabilistic approach. We will benchmark these approaches against the mutations originally identified using CRISP, a method optimized for pooled samples. Ultimately, quantifying the mutation load imposed by storage or growth on the ISS will help identify chassis organisms with both high levels of genome stability and viability, which are desirable traits for implementation of bioproduction in long-duration missions.

SNP↗
NASA NTRSDate not supplied

Bionutrients-1: Utilizing Genomics and Transcriptomics to Assess the Reliability of Microorganisms for In Situ Nutrient Production on Long Duration Missions

The resupply of current long-duration crewed missions to the ISS relies on ground-launched supplies. As NASA looks toward Mars, ground-based resupply will no longer be an option. Critical nutrients, including vitamin C, vitamin K, folate, and thiamin, degrade during long-term storage, and regular consumption of these nutrients is essential for astronaut health. Another challenge of current food systems is the difficulty of consuming sufficient calories when subsisting on the limited flavors of freeze-dried food, which can lead to weight loss. The inclusion of microorganism-based food systems could alleviate both concerns. For example, the fermentation of rehydrated milk into yogurt with microorganisms genetically incorporating genes to produce critical vitamins would allow for both in situ production of nutrients and a fresh food product with additional flavor profiles. In comparison to plant food production, microorganisms require less flight infrastructure. The BioNutrients-1 mission is demonstrating viability of microbial fermentation food production in microgravity and testing the reliability of this approach for long-duration missions lacking resupply. While the BioNutrients-1 mission includes the collection of multiple phenotypic measurements, this status update will focus on the processing of samples for genomics and transcriptomics analyses as well as the planned analysis pipelines. First, the BioNutrients-1 mission seeks to identify microorganisms capable of surviving long-duration storage at ambient temperatures while maintaining genetic fidelity. To achieve this, nine commonly employed microbial species were stored at ambient temperatures in Stasis Packs on the ISS for five years. The viability and mutation rates will be measured at multiple time points for both flown and ground control samples. From an omics perspective, the changes in the bulk rates of point mutations and genetic rearrangements across the Stasis Pack species during the five years of storage will be determined, providing valuable insights into the potential of these microorganisms for long-duration space missions. Second, the BioNutrients-1 mission is characterizing the impact of microgravity on fermentation. Two strains of the yeast Saccharomyces cerevisiae, each encoding antioxidants (β-carotene or zeaxanthin) were flown to ISS for storage and fermentation within simplified bioreactors (Production Packs). The impact of microgravity on the expression of the antioxidant production genes and general metabolic genes will be determined using RNA sequencing. Ultimately, the transcriptome data will be compared to phenotypic measurements, such as the antioxidant yield, end-state biomass, and the production of EtOH, to determine the impacts of microgravity and long-term storage on microbial fermentation. The findings from this research will be instrumental in understanding the challenges and opportunities of microorganism-based food systems in space missions.

BioNutrients↗
NASA NTRS1999

An Analysis of Gravity-Field Estimation Based on Intersatellite Dual-1-Way Biased Ranging

The GRACE (Gravity Recovery And Climate Experiment) mission is designed to make global, highly accurate measurements of the Earth's gravity field with high spatial resolution. Ancillary GPS occultation measurements are also to be carried out for atmospheric monitoring. In the dual-1-way biased ranging of this mission, the range between two satellites separated by 100 to 200 km in nearly polar, coplanar, circular orbits, is measured to very high precision, to within an additive constant, through the exchange of K- and Ka-band sinusoidal signals. Such biased ranging data, along with GPS L-band range and phase data, can be processed and fit over successive multiday intervals to obtain accurate estimates of the Earth's gravity field. This report approximately models and analyzes this process, from the generation of the RF signals at the two satellites through the extraction of the geopotential. The steps include generation of the transmitted signals, processing the received signals to extract high-rate baseband phase, carrying out a dual-1-way combination of baseband phase to extract high-rate biased range for each band, combining K- and Ka-band ranges to correct for the ionosphere effect, and processing the resulting high-rate biased range values to extract three types of reduced-rate observables: biased range, range rate and range acceleration. The version of dual-1-way biased ranging developed by this report improves upon previous versions in a number of ways: highly accurate satellite-timetag corrections derived from concurrent GPS data, better baseband phase extraction using highly digital processing, highly accurate USO-rate calibration derived from concurrent GPS data, an improved method for extracting high-rate biased range from baseband phase, improved filtering for extracting reduced- rate observables from high-rate biased range, and parallel extraction of three observable types.

SINUSOIDAL SIGNALS↗
NASA NTRS2003

Glucose-dependent insulinotropic peptide stimulates thymidine incorporation in endothelial cells: role of endothelin-1

We have previously characterized the receptor for glucose-dependent insulinotropic polypeptide (GIPR) in vascular endothelial cells (EC). Different EC types were found to contain distinct GIPR splice variants. To determine whether activation of the GIPR splice variants resulted in different cellular responses, we examined GIP effects on human umbilical vein endothelial cells (HUVEC), which contain two GIPR splice variants, and compared them with a spontaneously transformed human umbilical vein EC line, ECV 304, which contains four GIPR splice variants. GIP dose-dependently stimulated HUVEC and ECV 304 proliferation as measured by [3H]thymidine incorporation. GIP increased endothelin-1 (ET-1) secretion from HUVEC but not from ECV 304. Use of the endothelin B receptor blocker BQ-788 resulted in an inhibition of [3H]thymidine incorporation in HUVEC but not in ECV 304. These findings suggest that, although GIP increases [3H]thymidine incorporation in both HUVEC and ECV 304, this proliferative response is mediated by ET-1 only in HUVEC. These differences in cellular response to GIP may be related to differences in activation of GIPR splice variants.

NASA Discipline Clinical Medicine↗
NASA NTRS2004

DEMO: the Autonomous Sciencecraft Experiment onboard the EO-1 spacecraft

The Autonomous Sciencececraft Experiment (ASE), currently flying onboard the Earth Observing-1 (EO-1) spacecraft, integrates several autnomoy software technologies enabling autnomous science analysis and mission planning. The experiment demonstrates the potential for future space missions to use onboard decision-making to respond autonomously to capture short-lived science phenomena. The AAAI software demonstration will consist of two sections: a real-time display of an ASE-commanded ground contact from the EO-1 spacecraft, and a simulation of the full ASE autonomous science-response scenario.

EO-1 spacecraft↗
NASA NTRS2006

Considerations for Isochronous Data Services over the Proximity-1 Space Link

Future mission concepts for robotic and human explorations will involve a high level of real time control/monitoring operations such as tele-operation for spacecraft rendezvous and surface mobile platforms carrying life-support equipments. The timely dissemination of voice, command, and real-time telemetry for monitoring and coordination purposes is critical for mission success. It is envisioned that future missions will require a network infrastructure capable of supporting isochronous data services. The CCSDS Proximity-1 Space Link Protocol1 could be used to provide isochronous service over the surface-to-Earth relay as well as "beyond-the-horizon" communications between distant Lunar or Mars surface elements. This paper will analyze the latency, jitter, and throughput performance of the Proximity-1 protocol for isochronous applications. In particular we will focus on constrained scenarios where the protocol operates in full-duplex mode, carrying isochronous traffic in one direction and error-controlled traffic in the other direction. We analyze the impact of the strict priority scheme in Proximity-1 on delay jitter and the impact of the isochronous traffic on the efficiency of the reliable data transfer in the other direction, and discuss methods for performance optimization. In general, jitter performance is driving by relative loading of isochronous traffic on the forward link compared to the acknowledgement traffic. Under light loading condition, the upper-bound of the delay jitter is the transmission duration of an acknowledgement frame on the forward link; for higher loading scenarios, the maximum jitter is scaled up by the inverse of the residual bandwidth, i.e., the spare capacity available in the forward link to carry isochronous traffic.

isochronous communications↗
NASA NTRS2006

Considerations for Isochronous Data Services over the Proximity-1 Space Link

Future mission concepts for robotic and human explorations will involve a high level of real time control/monitoring operations such as tele-operation for spacecraft rendezvous and surface mobile platforms carrying life-support equipments. The timely dissemination of voice, command, and real-time telemetry for monitoring and coordination purposes is critical for mission success. It is envisioned that future missions will require a network infrastructure capable of supporting isochronous data services. The CCSDS Proximity-1 Space Link Protocol1 could be used to carry isochronous traffic. This paper we will focus on the data link layer portion of the Proximity-1 protocol specification and analyze its jitter and performance for supporting isochronous applications. In particular we will focus on constrained scenarios where the protocol operates in full-duplex mode, carrying isochronous traffic in one direction and error-controlled traffic in the other direction. We analyze the impact of the strict priority scheme in Proximity-1 used to arbitrate channel access on the latency jitter of the isochronous traffic and the efficiency of the reliable data transfer. In general, jitter performance is driven by the loading of the acknowledgement traffic on the forward link. Under light loading condition, the upper-bound of the delay jitter is the transmission duration of an acknowledgement frame on the forward link; for higher loading scenarios, the maximum jitter is scaled up by the inverse of the residual bandwidth, i.e., the spare capacity available in the forward link after accounting for the acknowledgement traffic. We derive analytical expression on the maximum jitter and discuss its performance.

space link↗
NASA NTRS2016

Integrating Chlorophyll fapar and Nadir Photochemical Reflectance Index from EO-1/Hyperion to Predict Cornfield Daily Gross Primary Production

The concept of light use efficiency (Epsilon) and the concept of fraction of photosynthetically active ration (PAR) absorbed for vegetation photosynthesis (PSN), i.e., fAPAR (sub PSN), have been widely utilized to estimate vegetation gross primary productivity (GPP). It has been demonstrated that the photochemical reflectance index (PRI) is empirically related to ε. An experimental US Department of Agriculture (USDA) cornfield in Maryland was selected as our study field. We explored the potential of integrating fAPAR(sub chl) (defined as the fraction of PAR absorbed by chlorophyll) and nadir PRI (PRI(sub nadir)) to predict cornfield daily GPP. We acquired nadir or near-nadir EO-1/Hyperion satellite images that covered the cornfield and took nadir in-situ field spectral measurements. Those data were used to derive the PRI(sub nadir) and fAPAR (sub chl). The fAPAR (sub chl) is retrieved with the advanced radiative transfer model PROSAIL2 and the Metropolis approach, a type of Markov Chain Monte Carlo (MCMC) estimation procedure. We define chlorophyll light use efficiency Epsilon (sub chl) as the ratio of vegetation GPP as measured by eddy covariance techniques to PAR absorbed by chlorophyll (Epsilon(sub chl) = GPP/APAR (sub chl). Daily Epsilon (sub chl) retrieved with the EO-1 Hyperion images was regressed with a linear equation of PRI (sub nadir) Epsilon (sub chl) = Alpha × PRI (sub nadir) + Beta). The satellite Epsilon(sub chl- PRI (sub nadir) linear relationship for the cornfield was implemented to develop an integrated daily GPP model [GPP = (Alpha × PRI(sub nadir) + Beta) × fAPAR (sub chl) × PAR], which was evaluated with fAPAR (sub chl) and PRI (sub nadir) retrieved from field measurements. Daily GPP estimated with this fAPAR (sub chl-) PRI (nadir) integration model was strongly correlated with the observed tower in-situ daily GPP (R(sup 2) = 0.93); with a root mean square error (RMSE) of 1.71 g C mol−(sup -1) PPFD and coefficient of variation (CV) of 16.57%. Both seasonal Epsilon (sub chl) and PRI (sub nadir) were strongly correlated with fAPAR (sub chl ) retrieved from field measurements, which indicates that chlorophyll content strongly affects seasonal epsilon (sub chl) and PRI (sub nadir). We demonstrate the potential capacity to monitor GPP with space-based visible through shortwave infrared (VSWIR) imaging spectrometers such as NASA's soon to be decommissioned EO- 1/Hyperion and the future Hyperspectral Infrared Imager (HyspIRI).

HyspIRI↗
NASA NTRS2014

Air Traffic Management Technology Demonstration 1 (ATD-1) Tech Transfer Document Summary: Version 3.0

This document summarizes transfer of NASA's terminal sequencing and spacing (TSS) and interval management (IM) technologies to the FAA (Federal Aviation Administration), as part of its Air Traffic Management Technology (ATM) Demonstration 1 activity. This activity, referred to as ATD-1, is part of NASA's Airspace Systems Program (ASP) specifically, its System Analysis, Integration, and Evaluation (SAIE) Project. ATD-1 is a multi-year research and development effort aimed at accelerating implementation and deployment of NASA-developed ATM technologies by the FAA. These technologies are designed to improve the utilization of Performance-Based Navigation (PBN) procedures inside congested terminal airspace. In terms of NASA's Technology Readiness Levels (TRLs), ATD-1 is focused on maturing its associated technologies from the Technology Development stage (TRL 4) to the Technology Demonstration stage (TRL 6). In order to ensure that the products of this tech-transfer are relevant and useful, NASA has created strong partnerships with the FAA and key industry stakeholders.

Technology transfer↗
NASA NTRS2015

Air Traffic Management Technology Demonstration (ATD-1) - Concept to Operations

In order to enable arrival management concepts and solutions in a NextGen environment, ground-based sequencing and scheduling functions have been developed to support metering operations in the National Airspace System. These sequencing and scheduling algorithms as well as tools are designed to aid air traffic controllers in developing an overall arrival strategy. The research presented provides an outline of the challenge of bringing time based scheduling to the terminal environment and the steps that were taken to bring the technology from concept to testing in an operational environment, and finally acquisition by the FAA. ATM Technology Demonstration-1 (ATD-1), is a set of technologies that provides an efficient solution for managing arrival aircraft beginning from just prior to top-of-descent and continuing down to the runway. The ATD-1 suite of technologies has been tested separately, and each has demonstrated benefits in throughput, delay, and fuel-efficiency. Together, the integration of terminal arrival tools allow arrival aircraft to safely fly closer together on more fuel-efficient routes to increase capacity, and reduce delay, fuel burn, noise, and greenhouse gas emissions.

DEMO↗
NASA NTRS2020

End-to-End Assessment of Artemis-1 Development Flight Instrumentation

As the space industry continues to strive for more efficient launch vehicles they must rely on increasingly accurate predictive models. Verification of models typically requires physical testing. Flight data measurements offer the most real and therefore the most accurate data for model correlation. As NASA prepares for the inaugural launch of their new Space Launch System (SLS), Artemis-1, they must rely heavily on predictive system models to ensure flight safety. Artemis-1 will be an unmanned scientific mission with the intent of blazing a trail for future manned missions. NASA has implemented a system of Development Flight Instrumentation (DFI) in the hopes of recovering useful flight data during liftoff and ascent to aid in correlating their predictive models to ensure human safety in future missions. An end-to-end assessment of the DFI system was performed to verify data acquired during Artemis-1 would be adequate for the targeted flight test objectives (FTOs). This was accomplished using a computational simulation of all sensors and Data Acquisition (DAQ) parameters to investigate any potential problem areas in the current architecture. Input nominal signals were approximated and injected into the system model. Synthesized acquired signals were recovered to verify FTO success.

Flight measurement↗
NASA NTRS2021

Segregation Evolution and Diffusion of Titanium in Directed Energy Deposited NASA HR-1

Titanium segregation in single-pass directed energy deposited (DED) NASA HR-1 is very difficult to control because grain size varies considerably and many large grains (>100 μm) are present. Therefore, homogenization kinetic analysis was performed to see how the homogenization process should be adjusted to mitigate titanium segregation. A basic model for titanium diffusion in NASA HR-1 was developed to project titanium segregation as a function of homogenization temperature, time, and grain size. The homogenization kinetic analysis provides a valuable reference on how the homogenization treatment should be adjusted to reduce titanium segregation to a very low level for DED NASA HR-1.

liquid rocket engine↗