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All sourcesNASA NTRSDOE OSTI
NASA NTRSDate not supplied

The ANGSA Program: A Low-cost Lunar "Sample Return Mission". An Overview and Progress over the Last 18 Months

The Apollo Program returned 381 kg of samples. Analyses of these samples have provided fundamental insights into the origin and history of the Earth-Moon system and how planets and even solar systems work. These samples have provided ground truth for every post-Apollo mission to the Moon for the interpretation of remotely sensed data. After 50 years of analysis and study, our sophistication for handling and examining samples has greatly increased. Some special samples that were collected or preserved in unique containers or environments remain unexamined by standard or advanced analytical approaches. The Apollo Next Generation Sample Analysis (ANGSA) Program was designed to examine a subset of these special samples. The ANGSA consortium consists of 9 original teams funded by NASA that have combined into a single science team referred to as the ANGSA Science Team. The program was designed to function as the sample analysis portion of a sample return mission with processing, preliminary examination, and analyses utilizing new and improved technologies and recent mission observations. The ANGSA Program links the first generation of lunar explorers (Apollo) with future explorers of the Moon (Artemis). The purpose of this abstract is to highlight the ANGSA samples, science and engineering goals, and progress made so far. Related abstracts and talks will focus on the links between Apollo and Artemis, geologic context, and initial examination, processing, and results.

F M McCubbin↗
DOE OSTI2023

Missing data in multi-omics integration: Recent advances through artificial intelligence

Biological systems function through complex interactions between various ‘omics (biomolecules), and a more complete understanding of these systems is only possible through an integrated, multi-omic perspective. This has presented the need for the development of integration approaches that are able to capture the complex, often non-linear, interactions that define these biological systems and are adapted to the challenges of combining the heterogenous data across ‘omic views. A principal challenge to multi-omic integration is missing data because all biomolecules are not measured in all samples. Due to either cost, instrument sensitivity, or other experimental factors, data for a biological sample may be missing for one or more ‘omic techologies. Recent methodological developments in artificial intelligence and statistical learning have greatly facilitated the analyses of multi-omics data, however many of these techniques assume access to completely observed data. A subset of these methods incorporate mechanisms for handling partially observed samples, and these methods are the focus of this review. We describe recently developed approaches, noting their primary use cases and highlighting each method's approach to handling missing data. We additionally provide an overview of the more traditional missing data workflows and their limitations; and we discuss potential avenues for further developments as well as how the missing data issue and its current solutions may generalize beyond the multi-omics context.

97 MATHEMATICS AND COMPUTING↗
NASA NTRS2018

Curating NASA's Past, Present, and Future Extraterrestrial Sample Collections

As codified in NASA Policy Directive 7100.10F, the Astromaterials Acquisition and Curation Office at NASA Johnson Space Center (hereafter JSC Curation) is charged with curation of all extraterrestrial material under NASA control, including future NASA missions. JSC Curation curates all or part of nine astromaterial collections in seven clean room suites: (1) Apollo Samples (1969; ISO 6-7), (2) Luna Samples (from USSR; 1972; ISO 7), (3) Antarctic Meteorites (1976; ISO 7), (4) Cosmic Dust (1981; ISO 5), (5) Microparticle Impact Collection (formerly called Space Exposed Hardware; 1985; ISO 5), (6) Genesis Solar Wind Atoms (2004; ISO 4); (7) Stardust Comet Particles (2006; ISO 5), (8) Stardust Interstellar Particles (2006; ISO 5), (9) Hayabusa Asteroid Particles (from JAXA; 2010; ISO 5). In addition to the labs that house the samples, we have installed and maintained a wide variety of facilities and infrastructure required to support the clean-rooms: more than 10 different HEPA-filtered air-handling systems, ultrapure dry gaseous nitrogen systems, an ultrapure water system (UPW) and cleaning facilities to provide clean tools and equipment for the labs. We also have sample preparation facilities for making thin sections, microtome sections, and even focused ion-beam (FIB) sections to meet the research requirements of scientists. To ensure that we are keeping the samples as pristine as possible, we routinely monitor the cleanliness of our clean rooms and infrastructure systems. This monitoring includes: daily monitoring of the quality of our UPW, weekly airborne particle counts in the labs, monthly monitoring of the stable isotope composition of the gaseous N2 system, and annual measurements of inorganic or organic contamination in processing cabinets. We track within our databases the current and ever-changing characteristics of more than 250,000 individual samples across our various collections (including the 19,141 samples on loan to 433 Principal Investigators in 24 countries). The next sample return missions that NASA will participate in are Hayabusa2 and OSIRIS-REx (Origins Spectral Interpretation Resource Identification Security - Regolith Explorer). The designs for a new state-of-the-art suite of clean rooms to house these samples at JSC have been finalized. This includes separate ISO class 5 clean rooms to house each collection, a common ISO class 7 area for general use, an ISO class 7 microtome laboratory, and a separate thin section lab. Additionally, a new cleaning facility is being designed and procedures developed that will allow for enhanced cleaning of cabinets and tools in an inorganically, organically, and biologically clean manner. We are also designing a large multi-purpose Advanced Curation laboratory that will allow us to develop the techniques necessary to fully support the Hayabusa2 and OSIRIS-REx missions, as well as future possible sample return missions (e.g., Lunar Polar Volatiles, Mars, Comet Surface). A micro-CT (micro Computed Tomography) laboratory dedicated to the study of astromaterials has come online within JSC Curation, and we plan to add additional facilities that will enable non-destructive (or minimally-destructive) analyses of astromaterials in the near future (e.g., micro-XRF (micro X-Ray Fluorescence), confocal imaging Raman Spectroscopy). These facilities will be available to: (1) develop sample handling and storage techniques for future sample return missions, (2) be utilized by PET (Positron Emission Tomography) for future sample return missions, (3) for retroactive PET-style analyses of our existing collections, and (4) for periodic assessments of the existing sample collections.

Zeigler, Ryan A.↗
DOE OSTI2010

Calendar Year 2009 Groundwater Monitoring Report, U.S. Department of Energy, Y-12 National Security Complex, Oak Ridge, Tennessee

This report contains the groundwater and surface water monitoring data that were obtained during calendar year (CY) 2009 at the U.S. Department of Energy (DOE) Y-12 National Security Complex (hereafter referenced as Y-12) on the DOE Oak Ridge Reservation (ORR) in Oak Ridge, Tennessee. The CY 2009 monitoring data were obtained from wells, springs, and surface water sampling locations in three hydrogeologic regimes at Y-12. The Bear Creek Hydrogeologic Regime (Bear Creek Regime) encompasses a section of Bear Creek Valley (BCV) between the west end of Y-12 and the west end of the Bear Creek Watershed (directions are in reference to the Y-12 grid system). The Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime) encompasses the Y-12 industrial facilities and support structures in BCV. The Chestnut Ridge Hydrogeologic Regime (Chestnut Ridge Regime) encompasses a section of Chestnut Ridge directly south of Y-12. Section 2 of this report provides background information pertinent to groundwater and surface water quality monitoring in each hydrogeologic regime, including the topography and bedrock geology, surface water drainage, groundwater system, and extent of groundwater contamination. The CY 2009 groundwater and surface water monitoring data in this report were obtained from sampling and analysis activities implemented under the Y-12 Groundwater Protection Program (GWPP) managed by Babcock & Wilcox Technical Services Y-12, LLC (B&W Y-12) and from sampling and analysis activities implemented under several monitoring programs managed by Bechtel Jacobs Company LLC (BJC). Cooperative implementation of the monitoring programs directed by the Y-12 GWPP and BJC (i.e., coordinating sample collection and sharing data) ensures that the CY 2009 monitoring results fulfill requirements of all the applicable monitoring drivers with no duplication of sampling and analysis efforts. Section 3 of this report contains a summary of information regarding the groundwater and surface water sampling and analysis activities implemented under the Y-12 GWPP including sampling locations and frequency; quality assurance (QA)/quality control (QC) sampling; sample collection and handling; field measurements and laboratory analytes; data management and data quality objective (DQO) evaluation; and groundwater elevation monitoring. However, this report does not include equivalent QA/QC or DQO evaluation information regarding the groundwater and surface water sampling and analysis activities associated with the monitoring programs implemented by BJC. Such details are deferred to the respective programmatic plans and reports issued by BJC (see Section 3.0). Collectively, the groundwater and surface water monitoring data obtained during CY 2009 by the Y-12 GWPP and BJC address DOE Order 450.1A (Environmental Protection Program) requirements for monitoring groundwater and surface water quality in areas: (1) which are, or could be, affected by operations at Y-12 (surveillance monitoring); and (2) where contaminants from Y-12 are most likely to migrate beyond the boundaries of the ORR (exit pathway/perimeter monitoring). Section 4 of this report presents a summary evaluation of the monitoring data with regard to the respective objectives of surveillance monitoring and exit pathway/perimeter monitoring, based on the analytical results for the principal groundwater contaminants at Y-12: nitrate, uranium, volatile organic compounds (VOCs), gross alpha activity, and gross beta activity. Section 5 of this report summarizes the most pertinent findings regarding the principal contaminants, along with recommendations proposed for ongoing groundwater and surface water quality monitoring performed under the Y-12 GWPP. Narrative sections of this report reference several appendices. Figures (maps and diagrams) and tables (excluding data summary tables presented in the narrative sections) are in Appendix A and Appendix B, respectively. Appendix C contains construction details for the wells in each regime that were sampled during CY 2009 by either the Y-12 GWPP or BJC. Field measurements recorded during collection of the groundwater and surface water samples and results of laboratory analyses of the samples are in Appendix D (Bear Creek Regime), Appendix E (East Fork Regime and surrounding areas), and Appendix F (Chestnut Ridge Regime). Appendix G contains data for the QA/QC samples associated with monitoring performed in each regime by the Y-12 GWPP.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2011

Calendar Year 2010 Groundwater Monitoring Report, U.S. Department Of Energy Y-12 National Security Complex, Oak Ridge, Tennessee

This report contains the groundwater and surface water monitoring data that were obtained during calendar year (CY) 2010 at the U.S. Department of Energy (DOE) Y-12 National Security Complex (hereafter referenced as Y-12) on the DOE Oak Ridge Reservation (ORR) in Oak Ridge, Tennessee. The CY 2010 monitoring data were obtained from wells, springs, and surface water sampling locations in three hydrogeologic regimes at Y-12 (Figure A.1). The Bear Creek Hydrogeologic Regime (Bear Creek Regime) encompasses a section of Bear Creek Valley (BCV) between the west end of Y-12 and the west end of the Bear Creek Watershed (directions are in reference to the Y-12 grid system). The Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime) encompasses the Y-12 industrial facilities and support structures in BCV. The Chestnut Ridge Hydrogeologic Regime (Chestnut Ridge Regime) encompasses a section of Chestnut Ridge directly south of Y-12. Section 2 of this report provides background information pertinent to groundwater and surface water quality monitoring in each hydrogeologic regime, including the topography and bedrock geology, surface water drainage, groundwater system, and extent of groundwater contamination. The CY 2010 groundwater and surface water monitoring data in this report were obtained from sampling and analysis activities implemented under the Y-12 Groundwater Protection Program (GWPP) managed by Babcock & Wilcox Technical Services Y-12, LLC (B&W Y-12) and from sampling and analysis activities implemented under several monitoring programs managed by Bechtel Jacobs Company LLC (BJC). Cooperative implementation of the monitoring programs directed by the Y-12 GWPP and BJC (i.e., coordinating sample collection and sharing data) ensures that the CY 2010 monitoring results fulfill requirements of all the applicable monitoring drivers with no duplication of sampling and analysis efforts. Section 3 of this report contains a summary of information regarding the groundwater and surface water sampling and analysis activities implemented under the Y-12 GWPP including sampling locations and frequency; quality assurance (QA)/quality control (QC) sampling; sample collection and handling; field measurements and laboratory analytes; data management and data quality objective (DQO) evaluation; and groundwater elevation monitoring. However, this report does not include equivalent QA/QC or DQO evaluation information regarding the groundwater and surface water sampling and analysis activities associated with the monitoring programs implemented by BJC. Such details are deferred to the respective programmatic plans and reports issued by BJC (see Section 3.0). Collectively, the groundwater and surface water monitoring data obtained during CY 2010 by the Y-12 GWPP and BJC address DOE Order 450.1A (Environmental Protection Program) requirements for monitoring groundwater and surface water quality in areas: (1) which are, or could be, affected by operations at Y-12 (surveillance monitoring); and (2) where contaminants from Y-12 are most likely to migrate beyond the boundaries of the ORR (exit pathway/perimeter monitoring). Section 4 of this report presents a summary evaluation of the monitoring data with regard to the respective objectives of surveillance monitoring and exit pathway/perimeter monitoring, based on the analytical results for the principal groundwater contaminants at Y-12: nitrate, uranium, volatile organic compounds (VOCs), gross alpha activity, and gross beta activity. Section 5 of this report summarizes the most pertinent findings regarding the principal contaminants, along with recommendations proposed for ongoing groundwater and surface water quality monitoring performed under the Y-12 GWPP.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2022

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments

We discuss the need to understand materials exposed to overlapping extreme environments such as high temperature, radiation, or mechanical stress. When these stressors are combined there may be synergistic effects that enable unique microstructural evolution mechanisms to activate. Understanding of these mechanisms is necessary for the input and refinement of predictive models and critical for engineering of next generation materials. The basic physics and underlying mechanisms require advanced tools to be investigated. The in situ ion irradiation transmission electron microscope (I 3 TEM) is designed to explore these principles. To quantitatively probe the complex dynamic interactions in materials, careful preparation of samples and consideration of experimental design is required. Particular handling or preparation of samples can easily introduce damage or features that obfuscate the measurements. There is no one correct way to prepare a sample; however, many mistakes can be made. The most common errors and things to consider are highlighted within. The I 3 TEM has many adjustable variables and a large potential experimental space, therefore it is best to design experiments with a specific scientific question or questions in mind. Experiments have been performed on large number of sample geometries, material classes, and with many irradiation conditions. The following are a subset of examples that demonstrate unique in situ capabilities utilizing the I 3 TEM. Au nanoparticles prepared by drop casting have been used to investigate the effects of single ion strikes. Au thin films have been used in studies on the effects of multibeam irradiation on microstructure evolution. Zr films have been exposed to irradiation and mechanical tension to examine creep. Ag nanopillars were subjected to simultaneous high temperature, mechanical compression, and ion irradiation to study irradiation induced creep as well. These results impact fields including: structural materials, nuclear energy, energy storage, catalysis, and microelectronics in space environments.

47 OTHER INSTRUMENTATION↗
NASA NTRS1992

Recommended practices for in-space and ground laboratory. Atomic oxygen exposure and analysis

A detailed guide to testing materials for atomic oxygen durability in earth orbit environments is presented. The steps covered include sample preparation, including masking of the sample, dehydration, weighing, and handling; effective fluence prediction, including the use of witness samples (notably Kapton); plasma facility and operational considerations, involving such matters as avoidance of silicone contamination, the use of continuous versus incremental ashing, and temperature of operation; and erosion yield measurement, with calculation methods and protective coating performance indices provided.

Banks, Bruce↗
NASA NTRS1999

Bring'em Back Alive or at Least Carefully

Within the next decade, the world's space agencies plan to launch a variety of robotic spacecraft that will return samples from the surface of Mars, the tail of a comet, the nucleus of a comet, the surface of an asteroid, and the solar wind. Most of these places are not considered likely spots for life, but any mission returning from a location with the potential for harboring life will require special containment and handling because of the possible inclusion of living entities within returned samples. In its 1997 report on sample return from Mars, the Space Studies Board of the National Research Council (NRC) noted that the only risk of significant adverse effects would be from returning a replicating organism. Furthermore, the report noted: 'While the probability of returning a replicating biological entity in a sample from Mars' is judged to be low and the risk of pathogenic or ecological effects is lower still, the risk is not zero. Therefore, it is reasonable that NASA adopt a prudent approach, erring on the side of caution and safety when dealing with returned samples. More recently, a 1998 NRC report on small solar system bodies (asteroids, comets, planetary satellites, and interplanetary dust) recommended a similarly cautious approach for samples returned from anywhere else within the solar system that could have environmental conditions conducive for harboring life. We have not detected life elsewhere in the solar system, at least not yet. Nonetheless, the rationale behind the conservative approach to sample handling is similar to the environmental, health, and safety measures taken on Earth when transporting or handling infectious agents or importing non-native organisms to a new area. Better safe than sorry.

Race, Margaret S.↗
NASA NTRSDate not supplied

Mars Sample Receiving Facility Research and Development to Enable Preservation, Safe Containment, and Scientific Research of Martian Samples on Earth

NASA and ESA are working together to plan a joint campaign to potentially bring back the first Martian samples to Earth in the early 2030s. On the surface of Mars, the Mars 2020Perseverance Rover is selecting and packaging samples that could be returned to Earth for careful examination by an international team of scientists. In preparation for this historic sample return, advance planning is underway to design and build a Mars Sample Receiving Facility (SRF) in the mid-2020s. In 2022, a Mars Sample Receiving Facility Assessment Study(MSAS) will be conducted to further define construction modality and capability options for a facility in the conterminous United States. Since Mars Sample Return (MSR) is currently categorized as a planetary protection restricted Earth return, this new facility would feature biosafety level 4 (BSL-4)-like high containment to protect Earth’s biosphere from any potential hazard. In addition, the facility also requires integration of cleanroom technologies to mitigate against terrestrial contamination and preserve sample integrity for science investigations. The integration of both clean handling and high containment requires significant technology research and development (R&D) by the mid-2020s to support the definition and planning of a SRF before commencing the site specific design phase. The SRF project will need to solidify the inorganic, organic, and biological contamination control (CC) requirements for the facility. This could entail a translation of Mars 2020 mission CC requirements for geologic material to engineering requirements for surfaces and airborne molecular contamination inside isolators and cleanrooms. In tandem, the project will need to determine precision cleaning and sterilization approaches for cleanrooms, isolators, equipment, and tools. Initial material selections for the facility construction materials, cleanrooms, isolators, and BSL-4 suits/garments are an important activity. The recent construction of the OSIRIS-REx and Hayabusa2 curation cleanroom laboratories greatly benefited from early materials testing, before the design phase, to reduced organic outgassing and particulate shedding inside the laboratory. While the early biohazard investigations in the SRF may deem the samples safe to release to laboratories throughout the world, sterilization methods need to be defined to safely release Martian samples. One key aspect of enabling simultaneous sample containment and cleanliness is the concept of a Double-Walled Isolator (DWI) that ESA has been pursuing. Further R&D is needed about DWIs, sample handling, and instrumentation interfaces before beginning the SRF site specific design phase. A working isolator/DWI engineering model should be developed and fabricated. Isolator sizes, connections, pass-through antechambers, and configurations and basic interfaces with instruments and tools as well as the use of robotic, mechanical, and/or human manipulation need to be considered. Inert high purity gas supply (one-pass or recirculation) and other isolator utilities must be determined. Special accommodations for large instruments (e.g., XCT,SEM, etc.) need to be developed. Techniques for opening the sample tubes packaged by M2020and extracting the headspace gas while keeping samples pristine will be challenging, requiring additional R&D, and engagement of the M2020 project. In addition, careful micromanipulation for subdivision and packaging of samples will also need to be included. These combined R&D activities are important before commencing the SRF design phase to ensure safe biohazard containment, sample preservation, and science integrity for the samples that would be studied in the SRF and in laboratories around the world.

mars sample return↗
NASA NTRS2006

System Would Acquire Core and Powder Samples of Rocks

A system for automated sampling of rocks, ice, and similar hard materials at and immediately below the surface of the ground is undergoing development. The system, denoted a sample preparation, acquisition, handling, and delivery (SPAHD) device, would be mounted on a robotic exploratory vehicle that would traverse the terrain of interest on the Earth or on a remote planet. The SPAHD device would probe the ground to obtain data for optimization of sampling, prepare the surface, acquire samples in the form(s) of cores and/or powdered cuttings, and deliver the samples to a selected location for analysis and/or storage.

Bar-Cohen, Yoseph↗
NASA NTRSDate not supplied

Constraining the Hydration of X-Ray Amorphous and Clay Mineral Phases in Gale Crater, Mars

Clay minerals and salts in the Gale crater stratigraphy investigated by the Mars Science Laboratory (MSL) Curiosity rover preserve information about sediment sources, depositional environment, and post-depositional alteration history. The compositions of amorphous components in samples have bearing on the redox potential, pH, and chemistry of alteration fluids, and on the heterogeneity of these conditions. Interlayer clay hydration in present-day Gale crater could be an indication of high water/rock ratios of late-stage fluid alteration events, which increases the potential habitability of these post-depositional environments. The MSL Dynamic Albedo of Neutrons instrument (DAN) measures bulk hydration and here we attempt to determine the hydration states of both clay minerals and amorphous components throughout the Curiosity traverse to constrain the abundance of individual amorphous phases and determine if clay interlayer water is present today. These implications are also more broadly applicable to similar sedimentary successions outside Gale crater. In this study, we compare hydration results from DAN to mineralogical results from the MSL Chemistry and Mineralogy instrument (CheMin) for 16 different fluvio-lacustrine sample locations. These samples typically contain one or more hydrated crystalline phases, a hydrated X-ray amorphous fraction composed of multiple amorphous phases, and one or more clay mineral phases which CheMin has consistently measured as collapsed (i.e., no interlayer water). However, Gale samples are known to dehydrate during sample acquisition and handling, and because DAN measures hydration to a depth of tens of cm, we can test for the presence of clay interlayer water in situ by comparing DAN bulk hydration to CheMin mineralogy. We can also constrain the hydration of the samples’ amorphous fractions, and with geochemical data from the ChemCam and APXS instruments, this will allow us to better define the phases which compose this fraction.

S Czarnecki↗
DOE OSTI2015

The rotary zone thermal cycler: A low-power system enabling automated rapid PCR

In this study, advances in molecular biology, microfluidics, and laboratory automation continue to expand the accessibility and applicability of these methods beyond the confines of conventional, centralized laboratory facilities and into point of use roles in clinical, military, forensic, portable, and field-deployed applications. As a result, there is a growing need to adapt the unit operations of molecular biology such as aliquoting, centrifuging, mixing, and thermal cycling to compact, portable, low-power, and automation-ready formats. Here we present one such adaptation, the rotary zone thermal cycler (RZTC), a novel wheel-based device capable of cycling up to four different fixed-temperature blocks into contact with a stationary 4-microliter capillary-bound sample to realize 1-3 second transitions with steady state heater power of less than 10 W. We further demonstrate the utility of the RZTC for DNA amplification as part of a highly integrated rotary zone PCR (rzPCR) system using low-volume valves and syringe-based fluid handling to automate sample loading and unloading, thermal cycling, and between run cleaning functionalities in a compact, modular form factor. In addition to characterizing the performance of the RZTC and the efficacy of different online cleaning protocols, preliminary results are presented for rapid single-plex PCR, multiplex short tandem repeat (STR) amplification, and second strand cDNA synthesis.

47 OTHER INSTRUMENTATION↗
DOE OSTI2014

Sensitive Targeted Quantification of ERK Phosphorylation Dynamics and Stoichiometry in Human Cells without Affinity Enrichment

Mass spectrometry-based targeted quantification is a promising technology for site-specific quantification of posttranslational modifications (PTMs). However, a major constraint of most targeted MS approaches is the limited sensitivity for quantifying low-abundance PTMs, requiring the use of affinity reagents to enrich specific PTMs. Herein, we demonstrate the direct site-specific quantification of ERK phosphorylation isoforms (pT, pY, pTpY) and their relative stoichiometries using a highly sensitive targeted MS approach termed high-pressure, high-resolution separations with intelligent selection and multiplexing (PRISM). PRISM provides effective enrichment of target peptides within a given fraction from complex biological matrix with minimal sample losses, followed by selected reaction monitoring (SRM) quantification. The PRISM-SRM approach enabled direct quantification of ERK phosphorylation in human mammary epithelial cells (HMEC) from as little as 25 µg tryptic peptides from whole cell lysates. Compared to immobilized metal-ion affinity chromatography, PRISM provided >10-fold improvement in signal intensities, presumably due to the better peptide recovery of PRISM for handling small size samples. This approach was applied to quantify ERK phosphorylation dynamics in HMEC treated by different doses of EGF at both the peak activation (10 min) and steady state (2 h). At 10 min, the maximal ERK activation was observed with 0.3 ng/mL dose, whereas the maximal steady state level of ERK activation at 2 h was at 3 ng/ml dose, corresponding to 1200 and 9000 occupied receptors, respectively. At 10 min, the maximally activated pTpY isoform represented ~40% of total ERK, falling to less than 10% at 2 h. The time course and dose-response profiles of individual phosphorylated ERK isoforms indicated that singly phosphorylated pT-ERK never increases significantly, while the increase of pY-ERK paralleled that of pTpY-ERK. This data supports for a processive, rather than distributed, model of ERK phosphorylation. The PRISM-SRM quantification of protein phosphorylation illustrates the potential for simultaneous quantification of multiple PTMs.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
NASA NTRS1989

Lunar deep drill apparatus

Proposed as a baseline configuration, this rotary drill apparatus is designed to produce 100-mm diameter holes in the lunar surface at depths up to 50 meters. The drill is intended to acquire samples for scientific analysis, mineral resource location, calibration of electronic exploration devices, and foundation analysis at construction sites. It is also intended to prepare holes for emplacement of scientific instruments, the setting of structural anchors, and explosive methods in excavation and mining activities. Defined as a deep drill because of the modular drill string, it incorporates an automatic rod changer. The apparatus is teleoperated from a remote location, such as earth, utilizing supervisory control techniques. It is thus suitable for unmanned and man-tended operation. Proven terrestrial drilling technology is used to the extent it is compatible with the lunar environment. Augers and drive tubes form holes in the regolith and may be used to acquire loose samples. An inertial cutting removal system operates intermittently while rock core drilling is in progress. The apparatus is carried to the work site by a three-legged mobile platform which also provides a 2-meter feed along the hole centerline, an off-hole movement of approximately .5 meters, an angular alignment of up to 20 deg. from gravity vertical, and other dexterity required in handling rods and samples. The technology can also be applied using other carriers which incorporate similar motion capabilities. The apparatus also includes storage racks for augers, rods, and ancillary devices such as the foot-plate that holds the down-hole tooling during rod changing operations.

Source record↗
DOE OSTI2013

Microplasma source for optogalvanic spectroscopy of nanogram samples

The demand for analysis of smaller samples in isotopic ratio measurements of rare isotopes is continuously rising with the development of new applications, particularly in biomedicine. Interesting in this aspect are methods based on optogalvanic spectroscopy, which have been reported to facilitate both {sup 13}C-to-{sup 12}C and {sup 14}C-to-{sup 12}C ratio measurements with high sensitivity. These methods also facilitate analysis of very small samples, down to the microgram range, which makes them very competitive to other technologies, e.g., accelerator mass spectroscopy. However, there exists a demand for moving beyond the microgram range, especially from regenerative medicine, where samples consist of, e.g., DNA, and, hence, the total sample amount is extremely small. Making optogalvanic spectroscopy of carbon isotopes applicable to such small samples, requires miniaturization of the key component of the system, namely the plasma source, in which the sample is ionized before analysis. In this paper, a novel design of such a microplasma source based on a stripline split-ring resonator is presented and evaluated in a basic optogalvanic spectrometer. The investigations focus on the capability of the plasma source to measure the optogalvanic signal in general, and the effect of different system and device specific parameters on the amplitude and stability of the optogalvanic signal in particular. Different sources of noise and instabilities are identified, and methods of mitigating these issues are discussed. Finally, the ability of the cell to handle analysis of samples down to the nanogram range is investigated, pinpointing the great prospects of stripline split-ring resonators in optogalvanic spectroscopy.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
DOE OSTI2025

Modified Data Collection And Analysis Codes Of Using Tcm (thermal Conductivity Microscope) To Measure Thermal Conductivity And Diffusivity

The "data collection" basically involves setting up the thermal wave frequency, laser scan distance, and other parameters related to the experimental setup. The modification of this code is minor and the details of this code can be found in the earlier patent ("thermal conductivity microscope"). The "data analysis" instead, replaces the simplified analytical model by a more complete analytical model, and used a "thermoquadruple" method to solve the analytical model. The efficiency is orders of magnitude improved and the accuracy is also better. Meanwhile, the previous model can only handle a two-layer sample structure. The new, complete model can handle materials with multiple layers (any given number), which is necessary to handle post ion irradiated materials.

Hua, Zilong [Idaho National Laboratory (INL), Idah↗
NASA NTRS2019

Recent Developments in the Curation of Cold, Volatile-Rich Extraterrestrial Samples

In recent years, the study of samples from cold, potentially volatile-rich Solar System bodies has increased dramatically. Returned samples from low- or cryogenic-temperature regions are highly sensitive to ambient temperatures, pressures, and materials. In order to maximize the scientific utility of such samples, they must be returned, handled, and stored under conditions that minimize sample alteration and contamination. The Johnson Space Center (JSC) Astromaterials Acquisition and Curation Office (hereafter called the Curation Office) is currently developing the ability to curate cold, volatile-rich samples; this abstract summarizes these efforts for Apollo lunar samples, organic-rich meteorites, comet samples, and lunar polar samples.

Mitchell, J. L.↗
DOE OSTI2022

Research Trends and Applications of PMUs

This work is a survey of current trends in applications of PMUs. PMUs have the potential to solve major problems in the areas of power system estimation, protection, and stability. A variety of methods are being used for these purposes, including statistical techniques, mathematical transformations, probability, and AI. The results produced by the techniques reviewed in this work are promising, but there is work to be performed in the context of implementation and standardization. As the smart grid initiative continues to advance, the number of intelligent devices monitoring the power grid continues to increase. PMUs are at the center of this initiative, and as a result, each year more PMUs are deployed across the grid. Since their introduction, myriad solutions based on PMU-technology have been suggested. The high sampling rates and synchronized measurements provided by PMUs are expected to drive significant advancements across multiple fields, such as the protection, estimation, and control of the power grid. This work offers a review of contemporary research trends and applications of PMU technology. Most solutions presented in this work were published in the last five years, and techniques showing potential for significant impact are highlighted in greater detail. Being a relatively new technology, there are several issues that must be addressed before PMU-based solutions can be successfully implemented. This survey found that key areas where improvements are needed include the establishment of PMU-observability, data processing algorithms, the handling of heterogeneous sampling rates, and the minimization of the investment in infrastructure for PMU communication. Solutions based on Bayesian estimation, as well as those having a distributed architectures, show great promise. The material presented in this document is tailored to both new researchers entering this field and experienced researchers wishing to become acquainted with emerging trends.

42 ENGINEERING↗