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At least 361 records · Page 20

Bone demineralization

Crew members of the Gemini 4, Gemini 5, and Gemini 7 missions were compared regarding skeletal changes in three major anatomic sites with respect to changes in skeletal density during space flight. Bone-mass changes have been found for the command pilot and the pilot of each mission in the conventional os calcis section, in the combined sections covering 60 percent of the os calcis, and in hand phalanges 5-2 and 4-2. Comparison of radiographically determined losses in X-ray absorbence with X-ray absorbence losses in healthy young men subjected to bedrest immobilization for the same length of time showed that losses for the crewmembers exceeded losses for the bedrest subjects in all cases; this was an indication that restriction of body movement did not represent the only factor involved.

Mack, P. B.↗

Habituation and adaptation of the vestibuloocular reflex: a model of differential control by the vestibulocerebellum

We habituated the dominant time constant of the horizontal vestibuloocular reflex (VOR) of rhesus and cynomolgus monkeys by repeated testing with steps of velocity about a vertical axis and adapted the gain of the VOR by altering visual input with magnifying and reducing lenses. After baseline values were established, the nodulus and ventral uvula of the vestibulocerebellum were ablated in two monkeys, and the effects of nodulouvulectomy and flocculectomy on VOR gain adaptation and habituation were compared. The VOR time constant decreased with repeated testing, rapidly at first and more slowly thereafter. The gain of the VOR was unaffected. Massed trials were more effective than distributed trials in producing habituation. Regardless of the schedule of testing, the VOR time constant never fell below the time constant of the semicircular canals (approximately 5 s). This finding indicates that only the slow component of the vestibular response, the component produced by velocity storage, was habituated. In agreement with this, the time constant of optokinetic after-nystagmus (OKAN) was habituated concurrently with the VOR. Average values for VOR habituation were obtained on a per session basis for six animals. The VOR gain was adapted by natural head movements in partially habituated monkeys while they wore x 2.2 magnifying or x 0.5 reducing lenses. Adaptation occurred rapidly and reached about +/- 30%, similar to values obtained using forced rotation. VOR gain adaptation did not cause additional habituation of the time constant. When the VOR gain was reduced in animals with a long VOR time constant, there were overshoots in eye velocity that peaked at about 6-8 s after the onset or end of constant-velocity rotation. These overshoots occurred at times when the velocity storage integrator would have been maximally activated by semicircular canal input. Since the activity generated in the canals is not altered by visual adaptation, this finding indicates that the gain element that controls rapid changes in eye velocity in the VOR is separate from that which couples afferent input to velocity storage. Nodulouvulectomy caused a prompt and permanent loss of habituation, returning VOR time constants to initial values. VOR gain adaptation, which is lost after flocculectomy, was unaffected by nodulouvulectomy. Flocculectomy did not alter habituation of the VOR or of OKAN. Using a simplified model of the VOR, the decrease in the duration of vestibular nystagmus due to habituation was related to a decrement in the dominant time constant of the velocity storage integrator (1/h0).(ABSTRACT TRUNCATED AT 400 WORDS).

NASA Discipline Neuroscience↗

Experimental analysis of thread movement in bolted connections due to vibrations

The objective of this study was to identify the main design parameters contributing to loosening of bolts due to vibration and to identify their relative importance and degree of contribution to bolt loosening. Vibration testing was conducted on a shaketable with a controlled-random input in the dynamic testing laboratory of the Structural Test Division of MSFC. Test specimens which contained one test bolt were vibrated for a fixed amount of time and percentage of pre-load loss was measured. Each specimen tested implemented some combination of eleven design parameters as dictated by the design of experiment methodology employed. The eleven design parameters were: bolt size (diameter), lubrication on bolt, hole tolerance, initial pre-load, nut locking device, grip length, thread pitch, lubrication between mating materials, class of fit, joint configuration and mass of configuration. These parameters were chosen for this experiment because they are believed to be the design parameters having the greatest impact on bolt loosening. Two values of each design parameter were used and each combination of parameters tested was subjected to two different directions of vibration and two different g-levels of vibration. One replication was made for each test to gain some indication of experimental error and repeatability and to give some degree of statistical credibility to the data, resulting in a total of 96 tests being performed. The results of the investigation indicated that nut locking devices, joint configuration, fastener size, and mass of configuration were significant in bolt loosening due to vibration. The results of this test can be utilized to further research the complex problem of bolt loosening due to vibration.

Ramey, G. ED↗

Feasibility of Earthbound Motion in Lunar Gravity

BACKGROUND: Marginal stability of astronaut movement while performing lunar surface tasks has been well documented, and is clearly demonstrated in videos of falls, and near falls, during Apollo Lunar Extravehicular Activities. Referencing mission reports from Apollo 15 and 16 [1, 2], suspected causes for falls include: surface conditions, visibility, and gravitational effects (hypogravity). In this preliminary test, we employ the open-source biomechanical tool OpenSim [3, 4] to analyze the impact of lunar gravity (Lg) on two object-pickup motions performed by a single shirt-sleeved subject. Specifically, we attempt to answer the following questions based on an estimation of the Center of Mass Projection (CoMP) and ground reaction force Center of Pressure (COP) as it relates to the astronaut Base of Support (BOS) for 1g and Lg conditions: 1. Is the task motion, as performed in 1g, dynamically feasible in 1g and Lg? 2. Can we make the motion dynamically feasible in Lg by slowing it down? 3. Is the Lg COP equal to the 1g COP at a theoretically predicted reduction in motion speed? METHODS AND RESULTS: To answer the first question, the gravitational acceleration in the OpenSim model is modified from a nominal 9.81 m/s2 to 1.64 m/s2, and the 1g joint trajectory is input to an OpenSim based method [5] for estimating ground reaction forces and moments. From this method, the position of the CoMP and COP can be estimated and checked to see if they remain within a simulated BOS formed from the footprint of the OpenSim model to determine whether the motion is dynamically feasible. As expected, both of the motions were estimated to be feasible in a 1g environment, however, both motions had periods of infeasibility in Lg. It is well known that crew members make adjustments to motion trajectories in altered gravity fields to maintain balance. As a first step, we considered the simple adjustment of slowing the motion in Lg by a constant factor. This was accomplished by scaling the time stamps in the motion trajectory file by that factor. For the two motions considered, it was found that scale factors of 1.3 and 1.4 kept the COP just within the subject BOS. The CoMP is unchanged by the gravity field. Simple analysis of an inverted pendulum in the Lg environment, which generalizes to a general multibody system, leads to a theoretical prediction that a reduction in speed factor of √1g/Lg, or 2.445, will make the COP trajectory in Lg equivalent to that in 1g. When the above procedure was performed with a factor of 2.445, the estimated COP in Lg, was observed to be very close to that in 1g. In summary, we have developed a method for estimating the CoMP and COP in Lg, for subject motion collected in 1g. We believe this method can prove to be a valuable check and balance for simulated Lg training and testing by exposing potential simulator-induced artifacts that make the simulated task motion seem possible, when in fact, it would violate the above criteria. We also note that a reduction in task speed should tend the task motion towards stability, with a theoretical slowdown factor of √1g/Lg making the motion stability equal to that in 1g according to the CoMP and COP criteria.

R K Huffman↗

Effect of spaceflight on the functional, biochemical, and metabolic properties of skeletal muscle

This paper summarizes the effects of spaceflight on the functional, morphological, and biochemical properties of human and rodent skeletal muscle. The findings suggest that following as little as 5-6 in space there are deficits in both human and rodent motor capacity, strength, and endurance properties of skeletal muscle. The reduced strength is associated, in part, with a reduction in muscle mass as reflected in smaller cross-sectional areas of both fast- and slow-twitch fibers. Available evidence in animal models suggests that slow-twitch fibers are more sensitive to the atrophying process. Accompanying the atrophy is a transformation of slow to fast protein phenotype involving myosin heavy chain and sarcoplasmic reticulum protein isoforms. These transformations appear to be regulated, in part, by pretranslational processes. Data on the oxidative capacity of rodent skeletal muscle suggest a bias toward preferential utilization of carbohydrate as the primary substrate. These collective findings suggest that skeletal muscles comprised chiefly of slow fibers are highly dependent on gravity for the normal expression of protein mass and slow phenotype. Future studies need to focus on elucidating the mechanisms associated with the atrophy response, as well as identifying suitable exercise and other countermeasures capable of preserving the structural and functional integrity of skeletal muscle.

NASA Discipline Musculoskeletal↗

A New Method for Interfacing Unsuited Subjects to Overhead Suspension Partial Gravity Simulators

The purpose of performing unsuited testing as part of a reduced gravity extravehicular (EVA) suited human performance research program is to define baseline performance. These results are then coupled with suited test results to evaluate how the suit system affects human performance at reduced gravity. The primary drawback to this approach is that previous studies used notably different systems to interface suited and unsuited subjects to overhead-suspension, partial-gravity simulators. A spreader bar (SB) assembly previously used for unsuited tests allowed limited pitch and roll of the subject, whereas the gimbal for suited tests allowed more pitch and roll, although the mass distribution led to large moments of inertia in the yaw axis. It is hypothesized that use of the same methods for offload of both unsuited and suited subjects is needed to make meaningful comparisons. A new gimbal (GIM) was designed with the idea that it could function with both suited and unsuited subjects. GIM was designed to minimize mass and moments of inertia and to be adjustable to co-locate the 3 axes of rotation with the subject s center of gravity. OBJECTIVE: To evaluate human performance differences between SB and GIM. METHODS: Ten unsuited subjects were offloaded to 1/6-g using both interfaces. Subjects completed tasks including overground and treadmill ambulation, picking up objects, shoveling, postural stability, range of motion testing, and recovery from the kneeling and prone positions. Metabolic, biomechanical, and/or subjective data were collected based on task. RESULTS: Initial analyses suggest that subjects completed all tasks with lower levels of compensation and a more terrestrial approach to movement when suspended via GIM. With SB, subjects were not able to fall or get into a prone position and had increased difficulty both retrieving objects off the floor and with overground ambulation, especially at gait initiation, because they were unable to bend their torso. GIM shows promise as a new method.

Norcross, Jason↗

Characteristics of annealed p/n junctions between GaAs and Si(100)

Electrical characteristics of a GaAs(p)/Si(n) interface were determined from capacitance-voltage, current-voltage, and secondary ion mass spectroscopy (SIMS) measurements and compared to those on GaAs(p) epitaxial layers on GaAs(n) substrates. The comparison was made between the junctions as grown, and after an anneal at 850 C for 20 min in 10 percent forming gas under an As overpressure. For the GaAs/Si junction the ideality factor changed from 2 or larger to 1.5 and the apparent intercept voltage changed from 2.5 to 1.3 V after annealing. For the GaAs homojunction, the intercept voltage increased from 1.1 to 1.3 V. In addition, the excess current in the forward and reverse bias conditions dropped drastically in the heterojunction. No movement of the metallurgical junction was discernible to within the resolution capability of SIMS. The junction properties obtained by annealing suggest an atomic restructuring of the Si(100) interface during growth or annealing. These new results raise the possibility that the GaAs/Si interface can be made into an electrically viable junction and incorporated into active devices.

Unlu, M. S.↗

A New Method for Interfacing Unsuited Subjects to Overhead Suspension Partial Gravity Simulators

The purpose of performing unsuited testing as part of a reduced gravity extravehicular (EVA) suited human performance research program is to define baseline performance. These results are then coupled with suited test results to evaluate how the suit system affects human performance at reduced gravity. The primary drawback to this approach is that previous studies used notably different systems to interface suited and unsuited subjects to overhead-suspension, partial-gravity simulators. A spreader bar (SB) assembly previously used for unsuited tests allowed limited pitch and roll of the subject, whereas the gimbal for suited tests allowed more pitch and roll, although the mass distribution led to large moments of inertia in the yaw axis. It is hypothesized that use of the same methods for offload of both unsuited and suited subjects is needed to make meaningful comparisons. A new gimbal (GIM) was designed with the idea that it could function with both suited and unsuited subjects. GIM was designed to minimize mass and moments of inertia and to be adjustable to co-locate the 3 axes of rotation with the subject s center of gravity. OBJECTIVE: To evaluate human performance differences between SB and GIM. METHODS: Ten unsuited subjects were off-loaded to 1/6-g using both interfaces. Subjects completed tasks including overground and treadmill ambulation, picking up objects, shoveling, postural stability, range of motion testing, and recovery from the kneeling and prone positions. Metabolic, biomechanical, and/or subjective data were collected based on task. RESULTS: Initial analyses suggest that subjects completed all tasks with lower levels of compensation and a more terrestrial approach to movement when suspended via GIM. With SB, subjects were not able to fall or get into a prone position and had increased difficulty both retrieving objects off the floor and with overground ambulation, especially at gait initiation, because they were unable to bend their torso. GIM shows promise as a new method.

Norcross, Jason R.↗

Development and implementation of an in situ high-resolution isotopic microscope for measuring metabolic interactions in soil mesocosms (Final Report)

Many of our planet’s ecosystems rely on the activities of soil microbial communities. These microbes have significant and wide-ranging effects: they metabolize carbon and other nutrients, interact with plants and fungi, and perform other processes important to soil health. However, our ability to directly observe the enzymatic and metabolic activities of microbes within soil is currently limited, not only by the complexity of soil microbial communities themselves, but also by the lack of experimental tools to study them and their molecular interactions in situ. These challenges hinder our understanding of the life-sustaining processes of biomass decomposition and the manner in which it contributes to the movement of freed carbon within soil ecosystems. Here we aimed to develop a novel ultrahigh-resolution isotopic microscope that combines complementary imaging modalities to gain insights into metabolic cycling in soil. This final report covers both the portion of this work that was initially completed at the University of North Carolina at Chapel Hill from 2018 to 2019 (as award DE-SC0019012) and then from 2020 to 2022 (2023 in NCE) at the University of Massachusetts Chan Medical School. This goal was to build an integrated platform consisting of fluorescence microscopy, Raman microspectroscopy, and nanospray desorption electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (nanoDESI-FTICR-MS) to directly investigate microbial activities and molecular transformations occurring in soil by exploiting the use of both fluorescent labels and stable isotope probing. Specifically, our instrument was conceived as integrating: (A) fluorescence detection to localize soil microbes, identify bacteria taking up polysaccharides, and monitor gene expression of enzymes involved in decomposition; (B) Raman microspectroscopy to determine which microbes incorporate decomposition products into their biomass, as well as which decomposers are metabolically active; and (C) nanoDESI-FTICR-MS imaging to spatially probe, in real-time, the metabolites in the surrounding area, which we expect will reveal the distributions of the products of the enzymatic breakdown of polysaccharides, as well as specialized metabolites acting as cell-cell signals between decomposers. To enable these measurements, we exploited fluorescence-based probes to map the microbes that are enzymatically active (per A) and utilized stable isotope-labeled substrates to visualize both the microbial and molecular fate of decomposed biomass (B and C, respectively). To accomplish our goal, we combined the expertise of a multidisciplinary group of scientists to pursue the construction of this microscope and to investigate scientific questions that would be facilitated by such a capability. We aimed to employ this spatially informative, high-resolution isotopic microscope to visualize the critical steps of biomass degradation and the molecular fate of other environmentally relevant substrates within soil mesocosms. Accordingly, this technology will enhance our understanding of the microbial and metabolic interactions occurring within soil communities that are relevant to carbon degradation and other soil processes. The instrument has been developed and housed at EMSL, where it will be available to the entire EMSL User Base, enabling a variety of related DOE-relevant systems to be interrogated in the future by diverse scientific research groups.

59 BASIC BIOLOGICAL SCIENCES↗

Regulation of hematopoiesis in rats exposed to antiorthostatic hypokinetic/hypodynamia. II - Mechanisms of the 'anemia'

The cause of the red cell mass (RCM) deficit, which occurs in rats during suspenion, is investigated. The experimental conditions and procedures, in which male Sprague-Dawley rats are subjected to antiorthostatic hyypokinetic/hypodynamia and changes in RCM are monitored, are described. The influences of stress, reduced food and water consumption, and antiorhostatic posture on RCM are analyzed. Changes in body weight, RCM, radioiron incorporation, red blood cells (RBC), and reticulocytes, for the rats after head-down suspension are graphically presented; only the changes in RBC are related to the antiorthostatic posture. The data reveal that anemia is primarily caused by reduced food and water consumption and secondly by restricted movements.

Dunn, C. D. R.↗

SPRUCE Xylem Native Embolism and Leaf Traits of Picea mariana and Larix laricina, 2019

This data set contains measurements of native embolism in branch xylem and associated branch and leaf traits from Picea mariana (Black Spruce) and Larix laricina (Tamarack) from September-October 2019 at the SPRUCE experiment (Hanson et al. 2017). Data are presented in one comma-separated (*.csv) file. Native embolism, a measurement of in-situ embolism in the xylem tissue that blocks water movement, measurements were conducted at the end of the growing season on cut branches using the hydraulic pipette method (see Peters et al. 2023 for full method). In short, branch segments were connected to hydraulic apprentice and flow rates of perfusion liquid were measured using graduated pipettes and stopwatch. After initial conductance measurements, branch segments were flushed using vacuum infiltration and hydraulic conductance was remeasured to calculate the percent loss in conductance due to embolism (PLC). Three branch segments (distal, middle, and proximal) were measured from each branch representing different diameters size classes. This dataset also contains leaf area associated with each measured branch segment, calculate leaf mass per area (LMA), sapwood specific conductivity (Ks), leaf area specific conductivity (Kl) and the sapwood area to leaf area ratio (Huber value). Measurements were made on mature trees from all ten treatment enclosures. One branch from each of five trees per species were used from each enclosure where available. Some plots do not contain five individual Larix laricina, in which case all available trees were sampled. All measurements were conducted in late September/early October 2019, at the end of growing season but before Larix laricina needle senescence. There are 10 experimental plots at SPRUCE: five temperature treatments (+0, +2.25, +4.5, +6.75, +9°C) at ambient CO2, and the same five temperature treatments at elevated CO2 (+500 ppm). These data were collected after the treatments had been running in full for three years, meaning much of the material measured was grown under treatment conditions.

Huber value↗

The K2 M67 Study: Precise Mass for a Turnoff Star in the Old Open Cluster M67

We present a study of the bright detached eclipsing main-sequence binary WOCS 11028 (Sanders 617) in the open cluster M67. Although the binary has only one eclipse per orbital cycle, we show that the masses of the stars can be derived very precisely thanks to a strong constraint on the orbital inclination: M {sub A} = 1.222 ± 0.006M {sub ⊙} and M {sub B} = 0.909 ± 0.004M {sub ⊙}. We use a spectral energy distribution fitting method to derive characteristics of the component stars in lieu of the precise radii that would normally be derived from a doubly eclipsing binary. The deconvolution of the SEDs reveals that the brighter component of the binary is at the faint turnoff point for the cluster—a distinct evolutionary point that occurs after the convective core has been established and while the star is in the middle of its movement toward lower surface temperatures, before the so-called hook at the end of the main sequence. The measurements are in distinct disagreement with evolution models at solar metallicity: higher metal abundances are needed to reproduce the characteristics of WOCS 11028 A. We discuss the changes to model physics that are likely to be needed to address the discrepancies. The clearest conclusions are that diffusion is probably necessary to reconcile spectroscopic abundances of M67 stars with the need for higher metallicity models and that reduced strength convective overshooting is occurring for stars at the turnoff. At super-solar bulk metallicity, various indicators agree on a cluster age between about 3.5 and 4.0 Gyr.

36 MATERIALS SCIENCE↗

CFD modeling and simulation for corrosive wear of refractory in molten slag

The development of high-wear resistant refractories having minimal production costs is facilitated by characterizing the wear mechanisms associated with their corrosive wear. Static cup testing is a commonly used method for comparing the corrosion resistance performance of two or more refractory materials. Although the static cup test conditions are not as severe as dynamic tests, this study shows that the thermal gradient present within the system during heating and cooling stages serves to generate movement of the slag leading to mechanical wear. The thermal gradient within the refractory, and between the slag and the refractory, occurs during the ramp stage of the test and lasts until the soaking stage is reached bringing the system to a thermal equilibrium. Using computational fluid dynamics (CFD) capabilities embedded within ANSYS software, this study modelled and quantified the convection currents within the slag and associated shear stresses generated on the refractory walls due to the thermal gradient. A traditional ladle furnace was employed as a case study to verify the results of the studied CFD model. The corrosion rate of the refractory lining was found to depend on the mass transfer coefficient of the refractory dissolution into the slag, and a velocity term which governs the extent of corrosion at any given location. This velocity term is a function of slag viscosity, as well as the concentration gradient and/or temperature gradient at the triple points. In this study, wall shear stress was used as a reliable proxy for identifying high-velocity regions prone to excessive corrosive wear. Elevated wall shear stress near the slag/air and slag/molten steel interfaces align with observed corrosion grooves, which reflects the intensified corrosive wear at these locations.

Ramteke, Rajat Rajat Durgesh Ramteke [University o↗

The aileron as an aid to recovery from the spin

As part of a general investigation by the NACA of factors that affect the spin, the use of the aileron as an aid to recovery from the spin was studied. Tests of 10 different models, covering a wide range of mass distribution, were made in the NACA free-spinning tunnel to determine the effects of a large downward deflection of the outboard aileron and of normal angular deflections of the ailerons upon recovery characteristics. The results indicate that the direction of aileron setting, with or against the spin, which will aid recovery from the spin depends upon the airplane weight distribution. For monoplanes and for biplanes with lower-wing ailerons, ailerons with the spin will be favorable when the weight is distributed chiefly along the fuselage (single-engine airplanes) and ailerons against the spin will be favorable when the weight is distributed chiefly along the wings (multi engine airplanes). Downward movement of the outboard aileron through a large angle will not always be effective in aiding recovery, the effectiveness of such a movement also being dependent upon the weight distribution of the airplane.

Neihouse, A I↗

Lunar and Planetary Science XXXV: Martian Aeolian and Mass Wasting Processes: Blowing and Flowing

The session Martian Aeolian and Mass Wasting Processes: BLowing and Flowing included the following topics: 1) Three Decades of Martian Surface Changes; 2) Thermophysical Properties of Isidis Basin, Mars; 3) Intracrater Material in Eastern Arabia Terra: THEMIS, MOC, and MOLA Analysis of Wind-blown Deposits and Possible High-Inertia Source Material; 4) Thermal Properties of Sand from TES and THEMIS: Do Martian Dunes Make a Good Control for Thermal Inertia Calculations? 5) A Comparative Analysis of Barchan Dunes in the Intra-Crater Dune Fields and the North Polar Sand Sea; 6) Diluvial Dunes in Athabasca Valles, Mars: Morphology, Modeling and Implications; 7) Surface Profiling of Natural Dust Devils; 8) Martian Dust Devil Tracks: Inferred Directions of Movement; 9) Numerical Simulations of Anastomosing Slope Streaks on Mars; 10) Young Fans in an Equatorial Crater in Xanthe Terra, Mars; 11) Large Well-exposed Alluvual Fans in Deep Late-Noachian Craters; 12) New Evidence for the Formation of Large Landslides on Mars; and 13) What Can We Learn from the Ages of Valles Marineris Landslides on Martian Impact History?

Source record↗

Quantifying ataxia: ideal trajectory analysis--a technical note

We describe a quantitative method to assess repeated stair stepping stability. In both the mediolateral (ML) and anterioposterior (AP) directions, the trajectory of the subject's center of mass (COM) was compared to an ideal sinusoid. The two identified sinusoids were unique in each direction but coupled. Two dimensionless numbers-the mediolateral instability index (IML) and AP instability index (IAP)-were calculated using the COM trajectory and ideal sinusoids for each subject with larger index values resulting from less stable performance. The COM trajectories of nine nonimpaired controls and six patients diagnosed with unilateral or bilateral vestibular labyrinth hypofunction were analyzed. The average IML and IAP values of labyrinth disorder patients were respectively 127% and 119% greater than those of controls (p<0.014 and 0.006, respectively), indicating that the ideal trajectory analysis distinguishes persons with labyrinth disorder from those without. The COM trajectories also identify movement inefficiencies attributable to vestibulopathy.

Non-NASA Center↗

The impacts of warming on rapidly retreating high-altitude, low-latitude glaciers and ice core-derived climate records

Alpine glaciers in the low- and mid-latitudes respond more quickly than large polar ice sheets to changes in temperature, precipitation, cloudiness, humidity, and radiation. Many high-altitude glaciers are monitored by ground observations, aerial photography, and satellite-borne sensors. Regardless of latitude and elevation, nearly all nonpolar glaciers and ice caps are undergoing mass loss, which compromises the records of past climate preserved within them. Almost without exception, the retreat of these ice fields is persistent, and a very important driver is the recent warming of the tropical troposphere and oceans. Here we present data on the decrease in the surface area of four glaciers from low- to mid-latitude mountainous regions: the Andes of Peru and northern Bolivia, equatorial east Africa, equatorial Papua, Indonesia, and the western Tibetan Plateau. Climate records based on oxygen isotopic ratios (δ18O) measured in ice cores drilled from several glaciers in these regions reveal that the records from elevations below ~6000 m above sea level have been substantially modified by seasonal melting and the movement of meltwater through porous upper firn layers. Fortunately, δ18O records recovered from higher altitude sites still contain well-preserved seasonal variations to the surface; however, the projected increase in the rate of atmospheric warming implies that climate records from higher elevation glaciers will eventually also be degraded. A long-term ice core collection program on the Quelccaya ice cap in Peru, Earth's largest tropical ice cap, illustrates that the deterioration of its climate record is concomitant with the increase in mid-troposphere temperatures. The melting ice and resulting growth of proglacial lakes presents an imminent hazard to nearby communities. The accelerating melting of glaciers, if sustained, ensures the eventual loss of unique and irreplaceable climate histories, as well as profound economic, agricultural, and cultural impacts on local communities.

Low-latitude glaciers↗

The influence of water, diet, and temperature on 87 Sr/ 86 Sr in fin spines of juvenile Atlantic Sturgeon Acipenser oxyrinchus oxyrinchus

Analysis of microchemistry in fish fin spines offers a non-lethal approach to address key questions relating to spatial patterns and habitat use of fish. Reconstruction of life history is based on chemical changes within calcified structures with an underlying assumption of elemental incorporation relative to environmental concentration. However, information is limited on the relative influence of water and diet concentrations of elements to corresponding concentrations within fin spines. Strontium (Sr) is commonly used in trace element studies because of its predictable changes within watersheds, with recent applications showing utility of 87 Sr/ 86 Sr for greater spatial resolution. Therefore, this study evaluated the influence of water and diet 87 Sr/ 86 Sr on pectoral fin spines of Atlantic Sturgeon A. oxyrinchus oxyrinchus in controlled laboratory experiments. In the first experiment, fish were exposed to a control, water spiked with 0.10 mg/L of 87 Sr or diet spiked with 0.25 µg/g or 0.50 µg/g of 87 Sr in replicated tanks for 12 weeks. In a second experiment, to determine the time duration required to occupy a different environment for fin spine chemistry to change, fish were maintained in water- or diet-spiked treatments for 1, 2, 3, or 6 weeks. Additionally, to determine if temperature has an influence on 87 Sr/ 86 Sr in fin spines, fish were held at 15 and 25°C for 12 weeks. Fin spines were analyzed using laser ablation multi-collector inductively coupled plasma mass spectrometry (LA-MC-ICP-MS), and water and diet samples were analyzed using solution based ICP-MS. Results from Experiment 1 indicate water is the primary contributor and diet a secondary contributor of 87 Sr/ 86 Sr in pectoral fin spines of Atlantic Sturgeon. Results from Experiment 2 indicate fin spine 87 Sr/ 86 Sr can be influenced by water after 1 week and diet after 12 weeks. Additionally, although sample size was limited (n=3), water temperature did not have an effect on 87 Sr/ 86 Sr fin spine chemistry. In conclusion, these results provide guidance for interpretation of 87 Sr/ 86 Sr in pectoral fin spines of sturgeon and indicate usefulness of the technique for retrospectively estimating movement and habitat use.

59 BASIC BIOLOGICAL SCIENCES↗