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At least 361 records · Page 20

A 90GHz Bolometer Camera Detector System for the Green Bank Telescope

We describe a close-packed, two-dimensional imaging detector system for operation at 90GHz (3.3mm) for the 100 m Green Bank Telescope (GBT) This system will provide high sensitivity (<1mjy in 1s rapid imaging (15'x15' to 250 microJy in 1 hr) at the world's largest steerable aperture. The heart of this camera is an 8x8 close packed, Nyquist-sampled array of superconducting transition edge sensor bolometers. We have designed and are producing a functional superconducting bolometer array system using a monolithic planar architecture and high-speed multiplexed readout electronics. With an NEP of approx. 2.10(exp 17) W/square root Hz, the TES bolometers will provide fast linear sensitive response for high performance imaging. The detectors are read out by and 8x8 time domain SQUID multiplexer. A digital/analog electronics system has been designed to enable read out by SQUID multiplexers. First light for this instrument on the GBT is expected within a year.

Benford, Dominic J.↗

A 90GHz Bolometer Camera Detector System for the Green

We describe a close-packed, two-dimensional imaging detector system for operation at 90GHz (3.3 mm) for the 100m Green Bank Telescope (GBT). This system will provide high sensitivity (less than 1mJy in 1s) rapid imaging (15'x15' to 150 micron Jy in 1 hr) at the world's largest steerable aperture. The heart of this camera is an 8x8 close-packed, Nyquist-sampled array of superconducting transition edge sensor (TES) bolometers. We have designed and are producing a functional superconducting bolometer array system using a monolithic planar architecture and high-speed multiplexed readout electronics. With an NEP of approximately 2 x 10(exp -17) W/square root of Hz, the TES bolometers will provide fast, linear, sensitive response for high performance imaging. The detectors are read out by an 8x8 time domain SQUID multiplexer. A digital/analog electronics system has been designed to enable read out by SQUID multiplexers. First light for this instrument on the GBT is expected within a year.

Benford, Dominic J.↗

Three-Dimensional Transgenic Cell Models to Quantify Space Genotoxic Effects

In this paper we describe a three-dimensional, multicellular tissue-equivalent model, produced in NASA-designed, rotating wall bioreactors using mammalian cells engineered for genomic containment of mUltiple copies of defined target genes for genotoxic assessment. The Rat 2(lambda) fibroblasts (Stratagene, Inc.) were genetically engineered to contain high-density target genes for mutagenesis. Stable three-dimensional, multicellular spheroids were formed when human mammary epithelial cells and Rat 2(lambda) fibroblasts were cocultured on Cytodex 3 Beads in a rotating wall bioreactor. The utility of this spheroidal model for genotoxic assessment was indicated by a linear dose response curve and by results of gene sequence analysis of mutant clones from 400micron diameter spheroids following low-dose, high-energy, neon radiation exposure

Gonda, S.↗

Non-Target Effect for Chromosome Aberrations in Human Lymphocytes and Fibroblasts After Exposure to Very Low Doses of High LET Radiation

The relationship between biological effects and low doses of absorbed radiation is still uncertain, especially for high LET radiation exposure. Estimates of risks from low-dose and low-dose-rates are often extrapolated using data from Japanese atomic bomb survivor with either linear or linear quadratic models of fit. In this study, chromosome aberrations were measured in human peripheral blood lymphocytes and normal skin fibroblasts cells after exposure to very low dose (.01 - 0.2 Gy) of 170 MeV/u Si-28-ions or 600 MeV/u Fe-56-ions. Chromosomes were analyzed using the whole chromosome fluorescence in situ hybridization (FISH) technique during the first cell division after irradiation, and chromosome aberrations were identified as either simple exchanges (translocations and dicentrics) or complex exchanges (involving >2 breaks in 2 or more chromosomes). The curves for doses above 0.1 Gy were more than one ion traverses a cell showed linear dose responses. However, for doses less than 0.1 Gy, Si-28-ions showed no dose response, suggesting a non-targeted effect when less than one ion traversal occurs. Additional findings for Fe-56 will be discussed.

Hada, Megumi↗

Track Structure and the Biological Effectiveness of Accelerated Particles for the Induction of Chromosome Damage

Track structure models predict that at a fixed value of LET, particles with lower charge number, Z will have a higher biological effectiveness compared to particles with a higher Z. In this report we investigated how track structure effects induction of chromosomal aberration in human cells. Human lymphocytes were irradiated in vitro with various energies of accelerated iron, silicon, neon, or titanium ions and chromosome damage was assessed in using three color FISH chromosome painting in chemically induced PCC samples collected a first cell division post irradiation. The LET values for these ions ranged from 30 to195 keV/micron. Of the particles studied, Neon ions have the highest biological effectiveness for induction of total chromosome damage, which is consistent with track structure model predictions. For complex-type exchanges 64 MeV/ u Neon and 450 MeV/u Iron were equally effective and induced the most complex damage. In addition we present data on chromosomes exchanges induced by six different energies of protons (5 MeV/u to 2.5 GeV/u). The linear dose response term was similar for all energies of protons suggesting that the effect of the higher LET at low proton energies is balanced by the production of nuclear secondaries from the high energy protons.

George, K.↗

The Biological Effectiveness of Accelerated Particles for the Induction of Chromosome Damage: Track Structure Effects and Cytogenetic Signatures of High-LET Exposure

Track structure models predict that at a fixed value of LET, particles with lower charge number, Z will have a higher biological effectiveness compared to particles with a higher Z. In this report we investigated how track structure effects induction of chromosomal aberration in human cells. Human lymphocytes were irradiated in vitro with various energies of accelerated iron, silicon, neon, or titanium ions and chromosome damage was assessed in using three color FISH chromosome painting in chemically induced PCC samples collected a first cell division post irradiation. The LET values for these ions ranged from 30 to 195 keV/micrometers. Of the particles studied, Neon ions have the highest biological effectiveness for induction of total chromosome damage, which is consistent with track structure model predictions. For complex-type exchanges 64 MeV/ u Neon and 450 MeV/u Iron were equally effective and induced the most complex damage. In addition we present data on chromosomes exchanges induced by six different energies of protons (5 MeV/u to 2.5 GeV/u). The linear dose response term was similar for all energies of protons suggesting that the effect of the higher LET at low proton energies is balanced by the production of nuclear secondaries from the high energy protons. All energies of protons have a much higher percentage of complex-type chromosome exchanges than gamma rays, signifying a cytogenetic signature for proton exposures.

George, K.↗

Estimation of the Dose and Dose Rate Effectiveness Factor

Current models to estimate radiation risk use the Life Span Study (LSS) cohort that received high doses and high dose rates of radiation. Transferring risks from these high dose rates to the low doses and dose rates received by astronauts in space is a source of uncertainty in our risk calculations. The solid cancer models recommended by BEIR VII [1], UNSCEAR [2], and Preston et al [3] is fitted adequately by a linear dose response model, which implies that low doses and dose rates would be estimated the same as high doses and dose rates. However animal and cell experiments imply there should be curvature in the dose response curve for tumor induction. Furthermore animal experiments that directly compare acute to chronic exposures show lower increases in tumor induction than acute exposures. A dose and dose rate effectiveness factor (DDREF) has been estimated and applied to transfer risks from the high doses and dose rates of the LSS cohort to low doses and dose rates such as from missions in space. The BEIR VII committee [1] combined DDREF estimates using the LSS cohort and animal experiments using Bayesian methods for their recommendation for a DDREF value of 1.5 with uncertainty. We reexamined the animal data considered by BEIR VII and included more animal data and human chromosome aberration data to improve the estimate for DDREF. Several experiments chosen by BEIR VII were deemed inappropriate for application to human risk models of solid cancer risk. Animal tumor experiments performed by Ullrich et al [4], Alpen et al [5], and Grahn et al [6] were analyzed to estimate the DDREF. Human chromosome aberration experiments performed on a sample of astronauts within NASA were also available to estimate the DDREF. The LSS cohort results reported by BEIR VII were combined with the new radiobiology results using Bayesian methods.

Chappell, L.↗

Monochromatic Measurements of the JPSS-1 VIIRS Polarization Sensitivity

Polarization sensitivity is a critical property that must be characterized for spaceborne remote sensing instruments designed to measure reflected solar radiation. Broadband testing of the first Joint Polar-orbiting Satellite System (JPSS-1) Visible Infrared Imaging Radiometer Suite (VIIRS) showed unexpectedly large polarization sensitivities for the bluest bands on VIIRS (centered between 400 and 600 nm). Subsequent ray trace modeling indicated that large diattenuation on the edges of the bandpass for these spectral bands was the driver behind these large sensitivities. Additional testing using the National Institute of Standards and Technologies Traveling Spectral Irradiance and Radiance Responsivity Calibrations Using Uniform Sources was added to the test program to verify and enhance the model. The testing was limited in scope to two spectral bands at two scan angles; nonetheless, this additional testing provided valuable insight into the polarization sensitivity. Analysis has shown that the derived diattenuation agreed with the broadband measurements to within an absolute difference of about0.4 and that the ray trace model reproduced the general features of the measured data. Additionally, by deriving the spectral responsivity, the linear diattenuation is shown to be explicitly dependent on the changes in bandwidth with polarization state.

Monochromatic↗

Modeling the Interactive Effects of Spruce Beetle Infestation and Climate on Subalpine Vegetation

In the subalpine zone of the Rocky Mountains, climate change is predicted to result in an increase in the frequency and severity of spruce beetle outbreaks. Climate change itself may affect vegetation, potentially leading to changes in species composition. The direct and indirect effects of climate and disturbances on forest composition, biomass, and dynamics open the possibility for non-linear ecosystem responses. Modeling studies allow for the study of the interaction of these effects and their impact on the forest system. University of Virginia Forest Model Enhanced (UVAFME), an individual-based gap model that simulates forest dynamics and characteristics, is updated with a spruce beetle subroutine that calculates the probability for beetle infestation and potential mortalityof each tree on a plot. The updated model is then run with multiple scenarios that combine beetle infestation with current or altered climate at sites across the southern Rocky Mountains. Results show that spruce beetle infestations acted to facilitate competition with invading lower-elevation species, resulting in an increase in the biomass of historically lower elevation species and a further decline in Engelmann spruce biomass than occurred with solely bark beetle disturbance or solely climate change. We also found an initial enhancing effect between spruce beetle infestation and climate change; however, by the end of 100 yr of climate change and potential beetle infestation, climate had a dampening effect on spruce beetle infestation, through loss of host trees. These results are an important step in understanding the possible futures for vegetation of the Rocky Mountains as well as for spruce forests across the western United States and Canada.

Foster, Adrianna C.↗

A Methodology for Actuating RCS Jets in a Continuous, Time-Accurate CFD Simulation

Computational fluid dynamics simulations of Mars entry vehicle reaction control systems have been used to gain a better understanding of the wide range of flow phenomena encountered during various phases of flight. One aspect of this is simulating the system during controlled flight. Current practices model the system in a continuously actuated state, but this methodology is unable to accurately model the dynamic behavior of the system during EDL. One method to improve this is to create a coupled CFD-RBD-Control simulation. The first step to this is establishing a method of accurately actuating an RCS jet during a time accurate CFD simulation. This paper presents a method to achieve this actuation. There are three phases to the methodology: establishing the off state, creating a ramping function that will initialize the jet flow, and establishing the on state. A test case of a jet in a supersonic crossflow is implemented to establish the success of the methodology in being able to actuate a jet in the midst of an ongoing CFD simulation. It shows significant improvement in boundary condition responsiveness for linear and exponential ramping functions in comparison to the step function. A preliminary demonstration of the methodology is presented for the MSL vehicle which shows significant advantages for the implementation of a linear ramping function over a step function.

Alexandra Hickey↗

A Methodology for Actuating RCS Jets in a Continuous, Time-Accurate CFD Simulation

Computational fluid dynamics simulations of Mars entry vehicle reaction control systems have been used to gain a better understanding of the wide range of flow phenomena encountered during various phases of flight. One aspect of this is simulating the system during controlled flight. Current practices model the system in a continuously actuated state, but this methodology is unable to accurately model the dynamic behavior of the system during EDL. One method to improve this is to create a coupled CFD-RBD-Control simulation. The first step to this is establishing a method of accurately actuating an RCS jet during a time accurate CFD simulation. This paper presents a method to achieve this actuation. There are three phases to the methodology: establishing the off state, creating a ramping function that will initialize the jet flow, and establishing the on state. A test case of a jet in a supersonic crossflow is implemented to establish the success of the methodology in being able to actuate a jet in the midst of an ongoing CFD simulation. It shows significant improvement in boundary condition responsiveness for linear and exponential ramping functions in comparison to the step function. A preliminary demonstration of the methodology is presented for the MSL vehicle which shows significant advantages for the implementation of a linear ramping function over a step function.

Alexandra Hickey↗

NASA's CLARREO Pathfinder Mission: The Reflected Solar’s First SITSat

The Climate Absolute Radiance and Refractivity Observatory (CLARREO) Pathfinder mission will take reflected solar (RS) highly accurate measurements needed to monitor Earth’s climate and will be the first RS SI-traceable Satellite Sensor (SITSat). The mission includes a RS spectrometer that will be installed on the International Space Station (ISS) and take measurements for at least one year. CLARREO Pathfinder (CPF) will use on-orbit calibration to achieve an unprecedented high accuracy with SI-traceability and the inter-calibration of other on-orbit instruments. The spectrometer is based on the HyperSpectral Imager for Climate Science (HySICS) instrument developed by the University of Colorado/Laboratory for Atmospheric and Space Physics of Boulder, CO, USA. HySICS is being designed to have a radiometric uncertainty of 0.3% (1-sigma), a five to ten times improvement over existing spaceflight RS instruments. High accuracy SI-traceable measurements such as these are critical to develop long-term climate-quality data sets. Additionally, by measuring spectral reflectance with high accuracy the CPF instrument will serve as an on-orbit intercalibration radiometric reference for operational Earth-viewing sensors, such as the Clouds and Earth’s Radiant Energy System (CERES) broadband shortwave instrument and the Visible/Infrared Imaging Radiometer Suite (VIIRS). Two-axis pointing, a spectral range from 350 nm to 2300 nm, and a spectral resolution ≤6 nm enable CPF to provide nearly coincident temporal, spatial, angular, and spectral matching of intercalibration targets, with sampling sufficient to reduce random errors. The intercalibration method will refine knowledge of target sensors’ effective offsets, gain, non-linearity, spectral response, and polarization sensitivity (as is relevant). Calibrated reflectance and reflected radiance spectra will be distributed to the scientific community through a NASA Distributed Active Archive Center (DAAC). We will present an overview of the CLARREO Pathfinder mission, its anticipated impact on monitoring climate variability, and the novel CPF direct intercalibration approach.

climate↗

Response of joint dominated space structures

An approximate method is developed for estimating the transient response of nonlinear systems in terms of linearized modes of response. Its advantages are that it is computationally more efficient than the time integration method and that it is possible to view the design problem in the more traditional physical terms of modal response. The major drawback of the approximate method is loss of accuracy. It seems that both approximate methods and time integration have their roles in design. Approximate methods provide efficient tools for performing parametric studies and they supply physical insights into how to optimize system performance that are not easily inferred from strictly numerical methods. Time integration provides a method for assessing the accuracy of the approximate solution for key simulations and for fine tuning the final design. In the procedure presented the nonlinear system is approximated by an equivalent linear system in which the system parameters are constant over the range of transient response.

Source record↗

DNA Repair Defects and Chromosomal Aberrations

Yields of chromosome aberrations were assessed in cells deficient in DNA doublestrand break (DSB) repair, after exposure to acute or to low-dose-rate (0.018 Gy/hr) gamma rays or acute high LET iron nuclei. We studied several cell lines including fibroblasts deficient in ATM (ataxia telangiectasia mutated; product of the gene that is mutated in ataxia telangiectasia patients) or NBS (nibrin; product of the gene mutated in the Nijmegen breakage syndrome), and gliomablastoma cells that are proficient or lacking in DNA-dependent protein kinase (DNA-PK) activity. Chromosomes were analyzed using the fluorescence in situ hybridization (FISH) chromosome painting method in cells at the first division post irradiation, and chromosome aberrations were identified as either simple exchanges (translocations and dicentrics) or complex exchanges (involving >2 breaks in 2 or more chromosomes). Gamma irradiation induced greater yields of both simple and complex exchanges in the DSB repair-defective cells than in the normal cells. The quadratic dose-response terms for both simple and complex chromosome exchanges were significantly higher for the ATM- and NBS-deficient lines than for normal fibroblasts. However, in the NBS cells the linear dose-response term was significantly higher only for simple exchanges. The large increases in the quadratic dose-response terms in these repair-defective cell lines points the importance of the functions of ATM and NBS in chromatin modifications to facilitate correct DSB repair and minimize the formation of aberrations. The differences found between ATM- and NBS-deficient cells at low doses suggest that important questions should with regard to applying observations of radiation sensitivity at high dose to low-dose exposures. For aberrations induced by iron nuclei, regression models preferred purely linear dose responses for simple exchanges and quadratic dose responses for complex exchanges. Relative biological effectiveness (RBE) factors of all of the DNA repair-defective cell lines were smaller than those of normal cells, with the DNA-PK-deficient cells having RBEs near unity. To further investigate the sensitivity differences that were observed in ATM and NBS deficient cells, chromosomal aberrations were analyzed in normal lung fibroblast cells treated with KU-55933 (a specific ATM kinase inhibitor) or Mirin (an Mre11- Rad50-Nbs1 complex inhibitor involved in activation of ATM). We also performed siRNA knockdown of these proteins. Preliminary data indicate that chromosome exchanges increase in cells treated with the specific ATM inhibitor. Possible cytogenetic signatures of acute and low dose-rate gamma irradiation in ATM or nibrin deficient and suppressed cells will be discussed.

Hada, Megumi↗

Gust response analysis for cascades operating in nonuniform mean flows

The linearized unsteady aerodynamic response of a cascade of airfoils subjected to entropic, vortical, and acoustic gusts is analyzed. Field equations for the first-order unsteady perturbation flow are obtained by linearizing the full time-dependent mass, momentum, and energy conservation equations about a nonlinear, isentropic, and irrotational mean or steady flow. A splitting technique is then used to decompose the unsteady velocity field into irrotational and rotational parts leading to field equations for the unsteady entropy, rotational velocity, and irrotational velocity fluctuations that are coupled only sequentially. The entropic and rotational velocity fluctuations can be described in terms of the mean-flow drift and stream functions which can be computed numerically. The irrotational unsteady velocity is described by an inhomogeneous linearized potential equation which contains a source term that depends on the rotational velocity field. This equation is solved via a finite difference technique. Results are presented to indicate the status of the numerical solution procedure and to demonstrate the impact of blade geometry and mean blade loading on the aerodynamic response of cascades to vortical gust excitations. The analysis described leads to very efficient predictions of cascade unsteady aerodynamic phenomena making it useful for turbomachinery aeroelastic and aeroacoustic design applications.

Hall, Kenneth C.↗

Gust response analysis for cascades operating in nonuniform mean flows

The linearized unsteady aerodynamic response of a cascade of airfoils subjected to entropic, vortical, and acoustic gusts is analyzed. Field equations for the first-order unsteady perturbation flow are obtained by linearizing the full time-dependent mass, momentum, and energy conservation equations about a nonlinear, isentropic, and irrotational mean or steady flow. A splitting technique is then used to decompose the unsteady velocity field into irrotational and rotational parts leading to field equations for the unsteady entropy, rotational velocity, and irrotational velocity fluctuations that are coupled only sequentially. The entropic and rotational velocity fluctuations can be described in terms of the mean-flow drift and stream functions which can be computed numerically. The irrotational unsteady velocity is described by an inhomogeneous linearized potential equation which contains a source term that depends on the rotational velocity field. This equation is solved via a finite difference technique. Results are presented to indicate the status of the numerical solution procedure and to demonstrate the impact of blade geometry and mean blade loading on the aerodynamic response of cascades to vortical gust excitations. The analysis described leads to very efficient predictions of cascade unsteady aerodynamics phenomena making it useful for turbomachinery aeroelastic and aeroacoustic design applications.

Hall, Kenneth C.↗

Dynamic response for thermal control and measurement and fast radiation thermometry

A preliminary evaluation was made by ORNL of a two-color ratio pyrometer (TCRP) for temperature control in the Modular Electromagnetic Levitation (MEL) experiment. A discussion was presented by Eric Spjut at the 1987 NASA Non-Contact Temperature Measurement Workshop (NASA Conf. Publ. 2503, pp. 182-213) in which he described the non-linear characteristics of the time response of TCPs. Researchers replicated his model and results and note that the non-linear response behavior is minimized for small temperature steps at high temperatures. They then used the predicted response in a model for a proportional or integral feedback controller and predicted the control characteristics for heating and cooling a 5-mm diameter sphere of niobium at high (1500 to 2750 K) temperatures. The analysis shows that for a slow (25-ms) time response for a commercial RCRP, overshoots of several hundred kelvins will result from a 100-K decrease in the setpoint, and temperature tracking errors of 14 to 45 K will occur for control temperature ramps of 1000K/s. For a fast (greater than 0.1 ms) time response, the overshoot and ramp response errors are largely eliminated.

Shepard, R. L.↗