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At least 361 records · Page 20

Modeling Deicing Operations in Departure Scheduling using Fast Time Simulation

In winter snow conditions, aircraft need inspection for deicing service before takeoff. Deicing service is a procedure to remove frost, ice, slush, or snow from aircraft for safe operation. Deicing operations vary by airport in many ways. Some airports have designated deicing zones, whereas some use a closed runway or terminal area to perform the procedure. Nonetheless, deicing operations add extra workloads to controllers, and cause increased taxi traffic on the ground. NASA and Korea Aerospace Research Institute (KARI) have been collaborating to model deicing operations at Incheon International Airport (ICN). This paper describes the deicing model and the study of deicing operations in departure scheduling using fast time simulations. The deicing model uses a heuristic algorithm for deicing zone assignment. In the fast time simulations, the model uses probability distributions derived from actual operation data to model deicing request and deicing zone time. It is envisioned that such a deicing model can be useful in airport surface scheduling to provide decision support and improve traffic management performance in winter snow operations.

surface operation↗

Models, In situ, and Remote sensing of Aerosols (MIRA): Formation of an International Working Group

There is a natural partitioning of scientific interest amongst three specialties of aerosol research: modeling, in situ measurements, and remote sensing. The community sees enhanced measurement capabilities when these groups interact, and this strengthens the overall scientific impact on climate and air quality. The Models, In situ, and Remote sensing of Aerosols (MIRA) working group connects members of the different aerosol communities through collaborative projects. What is MIRA? MIRA is forum that fosters international collaborations amongst the aerosol specialties. MIRA is also a collection of interdisciplinary projects with clear goals that are pursued by small working groups. Finally, MIRA projects are generally characterized by requests for additional scientific data (both observational and modeled). Why? The purpose of MIRA is to contextualize both observations and model results through the encouragement of holistic projects and collaborations. How does MIRA differ from other projects? MIRA focuses on interdisciplinarity to improve measurements and their utility, so MIRA complements the activities of other groups. For example, ensemble model runs of AeroCom could be used in a MIRA project with greater robustness than a similar effort that uses single-model analyses. We first present a description of MIRA and how your project can become a part of the MIRA community. This is followed by brief descriptions of some of the current MIRA projects, which include the Mapping of Aerosol lidar ratios for CALIPSO (MAC), the Tables of Aerosol Optics (TAO), and the Harmonization of aerosol Assimilation Models and Retrievals (HAMR). Finally, we discuss the immediate science goals and organization of MIRA.

Gregory Schuster↗

Models, In situ, and Remote sensing of Aerosols (MIRA)

There is a natural partitioning of scientific interest amongst three specialties of aerosol research: modeling, in situ measurements, and remote sensing. The community sees enhanced measurement capabilities when these groups interact, and this strengthens the overall scientific impact on climate and air quality. The Models, In situ, and Remote sensing of Aerosols (MIRA) working group connects members of the different aerosol communities through collaborative projects. What is MIRA? MIRA is a forum that fosters international collaborations amongst the aerosol specialties. MIRA is also a collection of interdisciplinary projects with clear goals that are pursued by small working groups. Finally, MIRA projects are generally characterized by requests for additional scientific data (both observational and modeled). Why? The purpose of MIRA is to contextualize both observations and model results through the encouragement of holistic projects and collaborations. How does MIRA differ from other projects? MIRA focuses on interdisciplinarity to improve measurements and their utility, so MIRA complements the activities of other groups. For example, ensemble model runs of AeroCom could be used in a MIRA project with greater robustness than a similar effort that uses single-model analyses. We present a description of MIRA and brief descriptions of some of the current MIRA topics, which include Satellite-Assisted Particulate Matter (SAPM), Mapping of Aerosol lidar ratios for CALIPSO (MAC), Tables of Aerosol Optics (TAO), and the Harmonization of aerosol Assimilation Models and Retrievals (HAMR). Finally, we discuss the immediate science goals and organization of MIRA. See https://science.larc.nasa.gov/mira-wg/ for more information.

Travis Toth↗

Satellite-Assisted Particulate Matter (SAPM) for the Models, In situ, and Remote Sensing of Aerosols (MIRA) Working Group

Models, In situ, and Remote sensing of Aerosols (MIRA) is an international working group that provides a forum for collaborations amongst these three atmospheric aerosol communities. MIRA consists of a collection of interdisciplinary and independently funded Topic/Project groups with clear goals and generally characterized by requests for additional scientific data. The Satellite-Assisted Particulate Matter (SAPM) Topic group focuses on the study of fine particulate matter (PM2.5), as it is a major contributor to air pollution and negatively impacts human health. Our group aims to provide intercomparisons of various methods and techniques for estimating surface PM2.5 assisted by satellite remote sensors (passive and active), global aerosol models, and in situ aerosol measurements. The overall motivation of our work is to enhance PM2.5 coverage beyond in situ ground stations, which can be limited. In this presentation, we provide an overview of various PM2.5 estimation approaches by current SAPM team members, as each approach has its own strengths and limitations. One approach uses near-surface aerosol extinction retrievals from the spaceborne Cloud-Aerosol Lidar with Orthogonal Polarization (CALIOP) and an assumed value of mass extinction efficiency to derive PM2.5 concentrations (Toth et al 2019). A study using this method found a promising agreement (R=0.60; slope=0.89) with U.S. Environmental Protection Agency (EPA) in situ PM2.5 measurements over the contiguous United States (CONUS) for a 12-year (2007-2018) period (Toth et al 2022). In a different approach combining spaceborne lidar and a model, the Cloud Aerosol Transport System (CATS) lidar and Goddard Earth Observing System (GEOS) model are blended in an ensemble variational assimilation scheme to retrieve aerosol extinction and convert speciated mass concentrations to total PM2.5. A recent study (Matus et al in prep.) found agreement within 2 μg/m3 over the CONUS on the 2016 annual mean when compared to EPA ground-based measurements. Another study explored trends in city aerosol optical depth (AOD) from a spaceborne passive remote sensor, Moderate Resolution Imaging Spectroradiometer (MODIS), and found that these trends agree well with trends in city surface PM2.5, which provides confidence in the use of city AOD for city PM2.5 trend studies (Vohra et al 2021). In a follow-on study, steep and significant trends (~2.5 to 7.8% a-1) in AOD are found for several South Asian cities (e.g., Bangalore and Hyderabad), suggesting rapid growth in PM2.5 (Vohra et al 2022). Also in this presentation, we report results on our current collective SAPM study of India, a country that exhibits high levels of PM2.5 pollution. We show the temporal (i.e., seasonal) and spatial variability of PM2.5 over India using the CALIOP and CATS+model approaches, comparisons between the estimated PM2.5 from the retrieval schemes, and validation with in situ PM2.5 ground-based observations from the Central Pollution Control Board in India. This region provides us with an excellent case study to test our PM2.5 retrievals in heavily polluted scenes and over a complex and varying topography. The SAPM Topic group actively seeks international participants/collaborators in our group, including those working with in situ aerosol measurements (e.g., ACTRIS). We are interested in aerosol datasets in order to either improve our PM2.5 estimates from various approaches (e.g., using in situ mass scattering/absorption coefficient and aerosol hygroscopic properties for various aerosol species) and/or validate the PM2.5 estimates (e.g., using in situ ground-based PM2.5 concentrations).

Travis D Toth↗

Vector send operation for message-based communication

Methods and systems for conducting vector send operations are provided. The processor of a sender node receives a request to perform a collective send operation (e.g., MPI_Broadcast) from a user application, requesting a copy of data in one or more send buffers by sent to each of a plurality of destinations in a destination vector. The processor invokes a vector send operation from a software communications library, placing a remote enqueue atomic send command for each destination node of the destination vector in an entry of a transmit data mover (XDM) command queue in a single call. The processor executes all of the commands in the XDM command queue and writes the data in the one or more send buffers into each receive queue of each destination identified in the destination vector.

Byrne, John L.↗

Climate data system supports FIRE

The NASA Climate Data System (NCDS) at Goddard Space Flight Center is serving as the FIRE Central Archive, providing a centralized data holding and data cataloging service for the FIRE project. NCDS members are carrying out their responsibilities by holding all reduced observations and data analysis products submitted by individual principal investigators in the agreed upon format, by holding all satellite data sets required for FIRE, by providing copies of any of these data sets to FIRE investigators, and by producing and updating a catalog with information about the FIRE holdings. FIRE researchers were requested to provide their reduced data sets in the Standard Data Format (SDF) to the FIRE Central Archive. This standard format is proving to be of value. An improved SDF document is now available. The document provides an example from an actual FIRE SDF data set and clearly states the guidelines for formatting data in SDF. NCDS has received SDF tapes from a number of investigators. These tapes were analyzed and comments provided to the producers. One product which is now available is William J. Syrett's sodar data product from the Stratocumulus Intensive Field Observation. Sample plots from all SDF tapes submitted to the archive will be available to FSET members. Related cloud products are also available through NCDS. Entries describing the FIRE data sets are being provided for the NCDS on-line catalog. Detailed information for the Extended Time Observations is available in the general FIRE catalog entry. Separate catalog entries are being written for the Cirrus Intensive Field Observation (IFO) and for the Marine Stratocumulus IFO. Short descriptions of each FIRE data set will be installed into the NCDS Summary Catalog.

Olsen, Lola M.↗

TEAMER: FOSWEC Mooring Modeling and Analysis, Post Access Report and Data

Floating oscillating surge wave energy converters (FOSWECs) offer several advantages over bottom-hinged oscillating surge wave energy converters, including large wave potential at deep-water sites with fewer permitting and environmental concerns outside territorial waters. As a team, Stevens Institute of Technology, Virginia Tech and Resolute Marine Energy are designing a 100 kW FOSWEC with DOE support (2020-2021) for the PacWave test site "PacWave". The proposed FOSWEC consists of a floating platform, two pivoting flaps, and an innovative power-take-off (PTO). The distance between the two flaps is around half the typical wavelength, resulting in out-of-phase motion and a reduction in motion of the frame and mooring loads. The overall goal of the project is to design, build, deploy and analyze a 1:2 scale (100-kW annual averaged electrical power output) device with reduced levelized cost of energy (LCOE) and peak-to-average power ratio, through the co-design and control of the PTO, WEC, and floating platform. This submission includes a Post Access Report and data for the project of Mooring Modeling and Analysis for Floating Oscillating Surge Wave Energy Converter that Powers Marine Aquaculture of RFTS2 (request for technical support). The data are used to generate all figures in the Post Access Report. Project was a collaboration between Virginia Tech and the National Renewable Energy Lab, with funding from TEAMER.

16 TIDAL AND WAVE POWER↗

Data Access Technology Workshop Position Paper

This paper addresses the seven questions that were requested of attendees prior to attending the Data Access Technology Workshop that is scheduled for October 8-9,2002. Each of the questions is addressed in the following seven sections. The seven questions are: 1) What emerging technologies do you feel would best enhance user access to EOSDIS data and why? 2) How do you envision these technologies being used? How should they be used in combination with each other? 3) Can technologies be readily leveraged to achieve these results today? If not, when do you think they will be viable? 4) What investments do you think EOSDIS should make in these technologies today? Over the next 5 years? 5) What are reasonable cost expectations for leveraging these technologies as you've suggested? 6) What are the anticipated impacts on end users if these technologies are deployed, including investments required? 7) What unique researcher data access requirements need to be supported?

Hinke, Thomas H.↗

IMAGE Software Suite

The IMAGE Mission is generating a truely unique set of magnetospheric measurement through a first-of-its-kind complement of remote, global observations. These data are being distributed in the Universal Data Format (UDF), which consists of data, calibration, and documentation. This is an open dataset, available to all by request to the National Space Science Data Center (NSSDC) at NASA Goddard Space Flight Center. Browse data, which consists of summary observations, is also available through the NSSDC in the Common Data Format (CDF) and graphic representations of the browse data. Access to the browse data can be achieved through the NSSDC CDAWeb services or by use of NSSDC provided software tools. This presentation documents the software tools, being provided by the IMAGE team, for use in viewing and analyzing the UDF telemetry data. Like the IMAGE data, these tools are openly available. What these tools can do, how they can be obtained, and how they are expected to evolve will be discussed.

Gallagher, Dennis L.↗

Overview of the OECD-NEA Working Party on International Nuclear Data Evaluation Cooperation (WPEC)

The OECD Nuclear Energy Agency (NEA) Working Party on International Nuclear Data Evaluation Cooperation (WPEC) was established in 1989 to facilitate collaboration in nuclear data activities. Over its thirty year history, different Subgroups have been created to address topics in nearly every aspect of nuclear data, including: experimental measurements, evaluation, validation, model development, quality assurance of databases and the development of software tools. WPEC has recently completed activities on fission yield evaluation, the general nuclear database structure (GNDS) to replace the ENDF-6 format, methods to provide feedback to evaluation, studies of specific capture cross sections, new methods in thermal scattering kernel evaluation and the Collaborative International Evaluated Library Organisation (CIELO) Pilot Project. Ongoing activities in GNDS application programming interface (API) development, methods for covariance evaluation and quality assurance in nuclear data validation using the International Criticality Safety Benchmark Evaluation Project (ICSBEP) database are complemented by the work of two Expert Groups that oversee the High-Priority Request List (HPRL) for Nuclear Data and the continuous development of the GNDS. New activities on the use of integral experiments for nuclear data validation and adjustment, as well as the use of the Shielding Integral Benchmark Archive and Database (SINBAD) for validation have begun and will be coordinated alongside future Subgroups. After three decades we will review the status of WPEC, how it integrates other collections and activities organised by the NEA and how it dovetails with the initiatives of the IAEA and other bodies to effectively coordinate international activities in nuclear data.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

Nuclear Data Needs and Capabilities for Applications

In July 2014, DOE NP carried out a review of the US Nuclear Data Program. This led to several recommendations, including that the USNDP should “devise effective and transparent mechanisms to solicit input and feedback from all stakeholders on nuclear data needs and priorities.” The review also recommended that USNDP pursue experimental activities of relevance to nuclear data; the revised 2014 Mission Statement accordingly states that the USNDP uses “targeted experimental studies” to address gaps in nuclear data. In support of these recommendations, DOE NP requested that USNDP personnel organize a Workshop on Nuclear Data Needs and Capabilities for Applications (NDNCA). This Workshop was held at Lawrence Berkeley National Laboratory (LBNL) on 27-29 May 2015. The goal of the NDNCA Workshop was to compile nuclear data needs across a wide spectrum of applied nuclear science, and to provide a summary of associated capabilities (accelerators, reactors, spectrometers, etc.) available for the required measurements. The first two days of the workshop consisted of 25 plenary talks by speakers from 16 different institutions, on nuclear energy (NE), national security (NS), isotope production (IP), and industrial applications (IA). There were also shorter “capabilities” talks that described the experimental facilities and instrumentation available for the measurement of nuclear data. This was followed by a third day of topic-specific “breakout” sessions and a final closeout session. The agenda and copies of these talks are available online at http://bang.berkeley.edu/events/NDNCA/agenda. The importance of nuclear data to both basic and applied nuclear science was reflected in the fact that while the impetus for the workshop arose from the 2014 USNDP review, joint sponsorship for the workshop was provided by the Nuclear Science and Security Consortium, a UC-Berkeley based organization funded by the National Nuclear Security Administration (NNSA).

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

A RAM architecture for concurrent access and on-chip testing

A novel RAM architecture supporting concurrent memory access and on-chip testing (CMAT) is proposed. A large-capacity memory chip is decomposed into test neighborhoods (TNDs), each of which is tested independently. When there are data stored in a TND, the data are saved into a buffer before testing the TND, and the TND's contents are restored using buffered data after testing the TND. If an external request is not made to the TND, the request can be directed to the addressed memory cells. Otherwise, the buffered data can be loaded back into the TND, or the request is detoured to a corresponding buffer. By deriving an analytical model, the performance penalty and hardware overhead of the CMAT architecture are shown to be very small.

Liu, Jyh-Charn↗

Data management in NOAA

NOAA has 11 terabytes of digital data stored on 240,000 computer tapes. There are an additional 100 terabytes (TB) of geostationary satellite data stored in digital form on specially configured SONY U-Matic video tapes at the University of Wisconsin. There are over 90,000,000 non-digital form records in manuscript, film, printed, and chart form which are not easily accessible. The three NOAA Data Centers service 6,000 requests per year and publish 5,000 bulletins which are distributed to 40,000 subscribers. Seventeen CD-ROM's have been produced. Thirty thousand computer tapes containing polar satellite data are being copied to 12 inch WORM optical disks for research applications. The present annual data accumulation rate of 10 TB will grow to 30 TB in 1994 and to 100 TB by the year 2000. The present storage and distribution technologies with their attendant support systems will be overwhelmed by these increases if not improved. Increased user sophistication coupled with more precise measurement technologies will demand better quality control mechanisms, especially for those data maintained in an indefinite archive. There is optimism that the future will offer improved media technologies to accommodate the volumes of data. With the advanced technologies, storage and performance monitoring tools will be pivotal to the successful long-term management of data and information.

Callicott, William M.↗

Range-based memory system

A mechanism is provided for efficient coherence state modification of cached data stored in a range of addresses in a coherent data processing system in which data coherency is maintained across multiple caches. A tag search structure is maintained that identifies address tags and coherence states of cached data indexed by address tags. In response to a request from a device internal to or external from the coherence network, the tag search structure is searched to identify address tags of cached data for which the coherence state is to be modified and requests are issued in the data processing system to modify a coherence state of cached lines with the identified address tags. The request from the external device may specify a range of addresses for which a coherence state change is sought. The tag search structure may be implemented as search tree, for example.

97 MATHEMATICS AND COMPUTING↗

Data for EMSL Project 60929 from August 2023: PI Goemann MONet Request

Just as humans rely on a healthy gut microbiome for resilience to illness, plants rely on a healthy root microbiome for resilience to environmental abiotic stress (heat, drought). To achieve a healthy root microbiome, plants release carbon (C)-rich compounds as root exudates to stimulate microbial activity and increase local nutrient mineralization. However, the enhanced performance comes at a cost: up to 44% of a plant’s C can be lost to root exudates, diverting C from plant growth and respiration. Critical knowledge gaps include how the ‘C cost’ is managed and how root exudates alter the microbiome under different environmental conditions. In addition, historical climate conditions, particularly mean annual precipitation, is known to shape local soil microbiomes and alter their sensitivity to drought. Therefore, studies that better characterize the plant-microbe responses to environmental stress will aid in efforts to harness the microbiome to improve crop resilience. However, current knowledge gaps make it challenging to engineer beneficial plant-microbe interactions to improve plant productivity in agricultural systems and to predict how increased climate variability will alter terrestrial C fluxes and climate feedbacks. To fill this knowledge gap our research group at Montana State University – Bozeman is currently studying blue grama (Bouteloua gracilis), a prairie grass native across the Northern Great Plains, as a model for drought tolerance. Our goal is to investigate the above- and belowground responses of blue grama to drought and heat stress to improve our understanding of stress-induced carbon allocation and plant-microbe interactions. Most recently, we investigated the influence of climate history on the blue grama drought response. We collected soil from three blue grama-dominated sites (those proposed to sample here) across a 150 mm mean annual precipitation gradient in SW Montana, USA, to use as inoculum for a greenhouse drought experiment. Preliminary results indicate that soil climate history has a strong influence on the blue grama physiological response to drought as well as on the chemical composition of root exudates and rhizosphere microbiome composition. Metabarcoding data from this experiment is scheduled to be submitted to public databases within the next year. Having in-depth analyses of the soil biogeochemistry and metagenomic composition through the MONet project at each of the field sites associated with this experiment will allow us to link underlying ecological processes with observed patterns of plant growth and productivity at each site. In addition, we plan to utilize the MONet database for future meta-analyses to compare the genomic and biogeochemical signatures of our field sites to others across a wider precipitation gradient throughout the native range of blue grama. This will further provide critical insights into the mechanisms that drive ecosystem functioning and resilience to drought stress.

Peyton, Brent↗

Data for EMSL Project 60929 from August 2023: PI Goemann MONet Request

Just as humans rely on a healthy gut microbiome for resilience to illness, plants rely on a healthy root microbiome for resilience to environmental abiotic stress (heat, drought). To achieve a healthy root microbiome, plants release carbon (C)-rich compounds as root exudates to stimulate microbial activity and increase local nutrient mineralization. However, the enhanced performance comes at a cost: up to 44% of a plant’s C can be lost to root exudates, diverting C from plant growth and respiration. Critical knowledge gaps include how the ‘C cost’ is managed and how root exudates alter the microbiome under different environmental conditions. In addition, historical climate conditions, particularly mean annual precipitation, is known to shape local soil microbiomes and alter their sensitivity to drought. Therefore, studies that better characterize the plant-microbe responses to environmental stress will aid in efforts to harness the microbiome to improve crop resilience. However, current knowledge gaps make it challenging to engineer beneficial plant-microbe interactions to improve plant productivity in agricultural systems and to predict how increased climate variability will alter terrestrial C fluxes and climate feedbacks. To fill this knowledge gap our research group at Montana State University – Bozeman is currently studying blue grama (Bouteloua gracilis), a prairie grass native across the Northern Great Plains, as a model for drought tolerance. Our goal is to investigate the above- and belowground responses of blue grama to drought and heat stress to improve our understanding of stress-induced carbon allocation and plant-microbe interactions. Most recently, we investigated the influence of climate history on the blue grama drought response. We collected soil from three blue grama-dominated sites (those proposed to sample here) across a 150 mm mean annual precipitation gradient in SW Montana, USA, to use as inoculum for a greenhouse drought experiment. Preliminary results indicate that soil climate history has a strong influence on the blue grama physiological response to drought as well as on the chemical composition of root exudates and rhizosphere microbiome composition. Metabarcoding data from this experiment is scheduled to be submitted to public databases within the next year. Having in-depth analyses of the soil biogeochemistry and metagenomic composition through the MONet project at each of the field sites associated with this experiment will allow us to link underlying ecological processes with observed patterns of plant growth and productivity at each site. In addition, we plan to utilize the MONet database for future meta-analyses to compare the genomic and biogeochemical signatures of our field sites to others across a wider precipitation gradient throughout the native range of blue grama. This will further provide critical insights into the mechanisms that drive ecosystem functioning and resilience to drought stress.

Peyton, Brent↗

Data for EMSL Project 60929 from August 2023: PI Goemann MONet Request

Just as humans rely on a healthy gut microbiome for resilience to illness, plants rely on a healthy root microbiome for resilience to environmental abiotic stress (heat, drought). To achieve a healthy root microbiome, plants release carbon (C)-rich compounds as root exudates to stimulate microbial activity and increase local nutrient mineralization. However, the enhanced performance comes at a cost: up to 44% of a plant’s C can be lost to root exudates, diverting C from plant growth and respiration. Critical knowledge gaps include how the ‘C cost’ is managed and how root exudates alter the microbiome under different environmental conditions. In addition, historical climate conditions, particularly mean annual precipitation, is known to shape local soil microbiomes and alter their sensitivity to drought. Therefore, studies that better characterize the plant-microbe responses to environmental stress will aid in efforts to harness the microbiome to improve crop resilience. However, current knowledge gaps make it challenging to engineer beneficial plant-microbe interactions to improve plant productivity in agricultural systems and to predict how increased climate variability will alter terrestrial C fluxes and climate feedbacks. To fill this knowledge gap our research group at Montana State University – Bozeman is currently studying blue grama (Bouteloua gracilis), a prairie grass native across the Northern Great Plains, as a model for drought tolerance. Our goal is to investigate the above- and belowground responses of blue grama to drought and heat stress to improve our understanding of stress-induced carbon allocation and plant-microbe interactions. Most recently, we investigated the influence of climate history on the blue grama drought response. We collected soil from three blue grama-dominated sites (those proposed to sample here) across a 150 mm mean annual precipitation gradient in SW Montana, USA, to use as inoculum for a greenhouse drought experiment. Preliminary results indicate that soil climate history has a strong influence on the blue grama physiological response to drought as well as on the chemical composition of root exudates and rhizosphere microbiome composition. Metabarcoding data from this experiment is scheduled to be submitted to public databases within the next year. Having in-depth analyses of the soil biogeochemistry and metagenomic composition through the MONet project at each of the field sites associated with this experiment will allow us to link underlying ecological processes with observed patterns of plant growth and productivity at each site. In addition, we plan to utilize the MONet database for future meta-analyses to compare the genomic and biogeochemical signatures of our field sites to others across a wider precipitation gradient throughout the native range of blue grama. This will further provide critical insights into the mechanisms that drive ecosystem functioning and resilience to drought stress.

Peyton, Brent↗

Data for EMSL Project 60929 from August 2023: PI Goemann MONet Request

Just as humans rely on a healthy gut microbiome for resilience to illness, plants rely on a healthy root microbiome for resilience to environmental abiotic stress (heat, drought). To achieve a healthy root microbiome, plants release carbon (C)-rich compounds as root exudates to stimulate microbial activity and increase local nutrient mineralization. However, the enhanced performance comes at a cost: up to 44% of a plant’s C can be lost to root exudates, diverting C from plant growth and respiration. Critical knowledge gaps include how the ‘C cost’ is managed and how root exudates alter the microbiome under different environmental conditions. In addition, historical climate conditions, particularly mean annual precipitation, is known to shape local soil microbiomes and alter their sensitivity to drought. Therefore, studies that better characterize the plant-microbe responses to environmental stress will aid in efforts to harness the microbiome to improve crop resilience. However, current knowledge gaps make it challenging to engineer beneficial plant-microbe interactions to improve plant productivity in agricultural systems and to predict how increased climate variability will alter terrestrial C fluxes and climate feedbacks. To fill this knowledge gap our research group at Montana State University – Bozeman is currently studying blue grama (Bouteloua gracilis), a prairie grass native across the Northern Great Plains, as a model for drought tolerance. Our goal is to investigate the above- and belowground responses of blue grama to drought and heat stress to improve our understanding of stress-induced carbon allocation and plant-microbe interactions. Most recently, we investigated the influence of climate history on the blue grama drought response. We collected soil from three blue grama-dominated sites (those proposed to sample here) across a 150 mm mean annual precipitation gradient in SW Montana, USA, to use as inoculum for a greenhouse drought experiment. Preliminary results indicate that soil climate history has a strong influence on the blue grama physiological response to drought as well as on the chemical composition of root exudates and rhizosphere microbiome composition. Metabarcoding data from this experiment is scheduled to be submitted to public databases within the next year. Having in-depth analyses of the soil biogeochemistry and metagenomic composition through the MONet project at each of the field sites associated with this experiment will allow us to link underlying ecological processes with observed patterns of plant growth and productivity at each site. In addition, we plan to utilize the MONet database for future meta-analyses to compare the genomic and biogeochemical signatures of our field sites to others across a wider precipitation gradient throughout the native range of blue grama. This will further provide critical insights into the mechanisms that drive ecosystem functioning and resilience to drought stress.

Peyton, Brent↗