Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “Injection”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 361 records · Page 20

EGS Collab Experiment 1: 4850L Downhole Camera Surveys During Injection

This package includes data and footage from two rounds of downhole camera surveys performed at the Sanford Underground Research Facility (SURF) on the 4850 level. The exercise was performed once on 25 May 2018 and once on 21 December 2018. On May 25th, the first round was done during fluid injection at the 164-ft stimulation zone in the injection well (E1-I). On December 21st, the second round was carried out during fluid injection at the 142-ft stimulation zone. Prior to the injections, downhole instrumentation was removed from the production well (E1-P) to allow room for the downhole camera system. The water within E1-P was then lifted out by the application of air pressure and the downhole camera system was conveyed into the production well. Finally, the water was injected into E1-I and the camera was used to scan for jetting points, or fluid entry, in E1-P. There is a survey description in this package that further describes the procedure of the survey and the overall results. Additionally, there is a detailed analysis of the surveys in the form of a PowerPoint, which includes animations/visualizations from the camera footage, presents interpretations in detail, and provides some general conclusions. Three animations, along with the two video segments that show the jetting into E1-P, are also provided. The video footage was collected using a GeoVISION Dual-Scan Micro Video Camera, the specs of which are also included in this package as a resource.

15 GEOTHERMAL ENERGY↗

The Climate Response to Stratospheric Aerosol Geoengineering Can Be Tailored Using Multiple Injection Locations

By injecting different amounts of SO 2 at multiple different latitudes, the spatial pattern of aerosol optical depth (AOD) can be partially controlled. This leads to the ability to influence the climate response to geoengineering with stratospheric aerosols, providing the potential for design. In this work, we use simulations from the fully coupled whole-atmosphere chemistry climate model CESM1(WACCM) to demonstrate that by appropriately combining injection at just four different locations, 30°S, 15°S, 15°N, and 30°N, then three spatial degrees of freedom of AOD can be achieved: an approximately spatially uniform AOD distribution, the relative difference in AOD between Northern and Southern Hemispheres, and the relative AOD in high versus low latitudes. For forcing levels that yield 1–2°C cooling, the AOD and surface temperature response are sufficiently linear in this model so that the response to different combinations of injection at different latitudes can be estimated from single-latitude injection simulations; nonlinearities associated with both aerosol growth and changes to stratospheric circulation will be increasingly important at higher forcing levels. Optimized injection at multiple locations is predicted to improve compensation of CO 2 -forced climate change relative to a case using only equatorial aerosol injection (which overcools the tropics relative to high latitudes). The additional degrees of freedom can be used, for example, to balance the interhemispheric temperature gradient and the equator to pole temperature gradient in addition to the global mean temperature. Further research is needed to better quantify the impacts of these strategies on changes to long-term temperature, precipitation, and other climate parameters.

54 ENVIRONMENTAL SCIENCES↗

Process Variability Effects on Tensile Response in Injection Molded, Fluorinated Thermoplastics

The mechanical properties of fluorinated thermoplastics (i.e., tensile strength and elongation) can vary with changes in injection molding processing parameters. Four fluoropolymers are examined: poly(vinylidene fluoride) (PVDF) and random poly(vinylidene fluoride-co-chlorotrifluoroethylene) (PVDF-CTFE) with three CTFE concentrations. Dog bones were manufactured with various cylinder dwell times and mold cooling times to assess the manufacturing sensitivity to the tensile response. Dwell and cooling times increasingly impact mechanical performance as CTFE concentration increases. Specimens exhibit higher tensile strength as a function of injection order. The first injected specimen exhibits the lowest tensile strength and highest elongation in all copolymers. This trend becomes more pronounced among fluoropolymers with higher CTFE concentration and lower weight-averaged molecular weight. Parallel plate rheology was used to obtain the zero-shear viscosity as a function of material type, process, and injection order. We found that in the copolymers, the first injected sample exhibited a lower zero-shear viscosity than the next, which indicates a lower molecular weight in the first injected specimen. This phenomenon was not presented for the PVDF homopolymer. Copolymer mechanical uncertainties are hypothesized to result from the shorter molecular weight chains extruding out of the specimens' sides as a flash due to higher mobility with CTFE segments.

36 MATERIALS SCIENCE↗

Radial storage efficiency for CO 2 injection: Quantifying effectiveness of local flow control methods

Basin-scale geologic carbon sequestration will require hundreds of injection wells, each of which has costs related to property rights and regulatory requirements that correlate with the areal size of the carbon dioxide plume. These surface-footprint-related issues motivate maximizing storage efficiency radially away from each well. Radial storage efficiency (RSE) is defined here as the ratio of the volumetrically weighted carbon dioxide free-phase saturation within a given radius away from the injection well to the available pore space within that same radius. Maximizing RSE effectively minimizes the radial extent of the CO 2 plume. Optimizing RSE around individual wells requires local flow control injection strategies that can increase the uniformity of the filling of pore space around the well over the entire length of the perforated injection interval despite differences in local formation transmissivity. The goal of uniform filling of the storage reservoir starting from the well outward is to maximize carbon dioxide sweep and trapping locally outward from the well in all of the layers of the storage region before buoyancy forces predominate and drive carbon dioxide upward where it will spread laterally under lower-permeability layers. Example simulations of carbon dioxide injection into a layered storage system with and without local flow control are presented to show the advantage of uniformly filling all layers and how RSE can be used to quantify storage efficiency for the two different injection approaches. Published 2021. This article is a U.S. Government work and is in the public domain in the USA.

03 NATURAL GAS↗

Influence of the Carrier Gas Species on CaO-Gas Mixed Injection in the EAF Steelmaking Process

In the process of electric arc furnace (EAF) steelmaking, usually the lime powder is sprayed into the molten pool with air as carrier gas to reduce costs. However, because of the nitrogen content requirements in the steel, oxygen or oxygen to mix carbon dioxide was substituted as the carrier gas for the lime powder injection. Lime injection with a burner mode has been reported to promote 100 pct lime injection in the EAF and faster dissolution of lime, but few studies have been made on the effect of the carrier gas on lime powder injection. In this study, four different kinds of carrier gas were used to investigate the influence of carrier gas on lime powder injection in EAF steelmaking. In this study, a CFD model based on the discrete particle model and eddy-dissipation concept model was carried out to investigate the influence of carrier gas species on the CaO-gas mixed injection in EAF steelmaking. The interaction between carrier gas and particles was analyzed, and the influences of carrier gas on the shrouding CH{sub 4} were also analyzed. Results show that the decrease of CO{sub 2} content in the main carrier gas provides better efficiency of the shrouding combustion and more uniform particle distribution.

36 MATERIALS SCIENCE↗

CO 2 water-alternating-gas injection for enhanced oil recovery: Optimal well controls and half-cycle lengths

We report that CO 2 water-alternating-gas (WAG) injection is an enhanced oil recovery method designed to improve sweep efficiency during CO 2 injection with the injected water to control the mobility of CO 2 and to stabilize the gas front. Optimization of CO 2 -WAG injection is widely regarded as a viable technique for controlling the CO 2 and oil miscible process. Poor recovery from CO 2 -WAG injection can be caused by inappropriately designed WAG parameters. In previous study (Chen and Reynolds, 2016), we proposed an algorithm to optimize the well controls which maximize the life-cycle net-present-value (NPV). However, the effect of injection half-cycle lengths for each injector on oil recovery or NPV has not been well investigated. In this paper, an optimization framework based on augmented Lagrangian method and the newly developed stochastic-simplex-approximate-gradient (StoSAG) algorithm is proposed to explore the possibility of simultaneous optimization of the WAG half-cycle lengths together with the well controls. Finally, the proposed framework is demonstrated with three reservoir examples.

42 ENGINEERING↗

Injection and Monitoring at the Wallula Basalt Pilot Project

Continental flood basalts represent one of the largest geologic structures on earth but have received comparatively little attention for geologic storage of CO2. Flood basalt lava flows have flow tops that are porous, permeable, and have large potential capacity for storage of CO2. In appropriate geologic settings, interbedded sediment layers and dense low-permeability basalt rock flow interior sections may act as effective seals allowing time for mineralization reactions to occur. Previous laboratory experiments showed the relatively rapid chemical reaction of CO2-saturated pore water with basalts to form stable carbonate minerals. However, recent laboratory tests with water-saturated supercritical CO2 show that mineralization reactions occur in this phase as well, providing a second and potentially more important mineralization pathway than was previously understood. Field testing of these concepts is proceeding with drilling of the world’s first supercritical CO2 injection well in flood basalt being completed in May 2009 near the township of Wallula in Washington State and corresponding CO2 injection permit granted by the State of Washington in March 2011. Injection of a nominal 1000 MT of CO2 was completed in August 2013 and site monitoring is in progress. Well logging conducted immediately after injection termination confirmed the presence of CO2 predominantly within the upper flow top region, and showed no evidence of vertical CO2 migration outside the well casing. Shallow soil gas samples collected around the injection well show no evidence of leakage and fluid and gas samples collected from the injection zone show strongly elevated concentrations of Ca, Mg, Mn, and Fe and 13C/18O isotopic shifts that are consistent with basalt-water chemical reactions. If proven viable by this field test and others that are in progress or being planned, major flood basalts in the U.S., India, and perhaps Australia would provide significant additional CO2 storage capacity and additional geologic sequestration options in regions of these countries where conventional storage options are limited.

54 ENVIRONMENTAL SCIENCES↗

Real-time reduction of tungsten impurity influx using lithium powder injection in EAST

We report the first successful use of solid material injection, in powder form, to reduce core tungsten impurities in EAST discharges with tungsten divertor plasma-facing components. Tungsten is the leading plasma-facing material for use in ITER as well as future fusion devices. However, tungsten sometimes accumulates in the plasma core of fusion devices, which is an impediment to the achievement of high-power, long-pulse H-modes. In this work, tungsten impurity influx from the tungsten divertor was reduced by real-time injection of lithium powder into EAST discharges. An increase in stored energy and confinement accompanied the sharp reduction of core tungsten with real-time lithium powder injection in L-mode discharges. In H-mode discharges, real-time lithium powder injection reduced the tungsten core impurity emission while also mitigating ELMs. During powder injection, the divertor electron temperature was reduced, which reduced the tungsten source. In conclusion, the tungsten impurity emission remained low in discharges even after active lithium injection was terminated, a signature of improved and lasting wall conditioning in the tungsten divertor.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Experimental investigation of injection-induced fracturing during supercritical CO 2 sequestration

Leakage risk assessment is an inevitable procedure in permanent sequestration and storage of CO 2 in deep saline aquifers and depleted oil and gas reservoirs, where the integrity of caprock is most critical. Here, low porosity and low permeability concrete cubes were employed as caprock analogs to investigate the supercritical CO 2 injection-induced fracturing processes under true tri-axial stress conditions. A systematic experimental procedure, consisting of active acoustic emission measurement, pressure decay, injection pressure and temperature monitoring, fracture coloring, and gas fracturing, is formulated to qualitatively and quantitatively characterize the injection-induced fracturing processes and fracture morphology. Occurrence of injection-induced fracturing can be directly identified from peaks of borehole pressure profiles as well as sharp drops on temperature profiles due to CO 2 expansion, but generally there was no fracture propagation plateau appearing for the 20 cm rock cubes with zero pore pressure. Acoustic wave signatures, including both waveform change and arrival time delay, can effectively capture the extension of induced fractures inside the opaque rock. Initiation and propagation of supercritical CO 2 injection-induced fractures are highly dominated by the tri-axial stresses, following the general trend of continuum mechanics at high stress levels with large stress difference. As the stress difference decreases, induced fractures branch off in relatively arbitrary directions. For these supercritical CO 2 injection-induced fracturing experiments, poroelastic mechanics model makes a decent fit with the measured breakdown pressure of the caprock analogs. Findings in this study are valuable for risk analysis and operation optimization of geological CO 2 sequestration and storage as well as for CO 2 fracturing design and implementation in shale and tight reservoirs.

42 ENGINEERING↗

Machine learning to discover mineral trapping signatures due to CO 2 injection

Mineral trapping is pursued as a geological CO 2 sequestration (GCS) mechanism because it permanently stores CO 2 in solid phases or minerals. However, CO 2 mineral-trapping mechanisms are poorly understood due to (1) lack of sufficient field and laboratory data characterizing these complex processes, and (2) challenges to develop site-specific reactive-transport models coupling fluid flow and geochemical reactions occurring at various temporal (from milliseconds to years) and spatial (from pore (millimeters) to field (kilometers)) scales. Reactive transport with additional complexities such as heterogeneity can make the simulation outputs even more difficult to interpret because of complex nonlinearity and multi-scale interdependencies. Furthermore, the values of model outputs such as concentrations can vary by several orders of magnitude, making it harder to correlate and characterize the impact of the variables via traditional data interpretation techniques such as exploratory data analyses. Recently, machine learning (ML) has shown promise in feature discovery and in highlighting hidden mechanisms that cannot be obtained by existing data-analytics and statistical methods. In this study, we applied an unsupervised ML approach, non-negative matrix factorization with custom -means clustering (NMF) to the data generated by reactive-transport simulations of GCS. The reactive-transport data consisted of 19 attributes, including four physio-chemical variables (pH, porosity, aqueous CO 2 , and sequestered CO 2 ), six chemical species (K + , Na + , HCO, Ca 2+ , Mg 2+ , Fe 2+ ), and four carbonate minerals (calcite, dolomite, siderite, and ankerite), a feldspar mineral (albite), and four clay minerals (illite, clinochlore, kaolinite, and smectite) over a period of 200 years of simulation time. Furthermore, the simulation data used was for Morrow B sandstone at the Farnsworth hydrocarbon unit in Texas. Data are sampled at two locations within the model domain: (1) at the injection well and (2) 200 m west of the injection well. The injection was performed for a period of 10 years. Using NMF, we estimated the temporal interdependencies among the 19 attributes over a span of 200 years. We found that NMF was able to identify four reaction stages and their dominant attributes; these cannot be directly discerned through traditional visualization (e.g., line plots, Pareto analysis, Glyph-based visualization methods) or exploratory data analysis tools of the simulation data. The four stages were: reactions in the injection phase followed by short-, mid-, and long-term reactions. The NMF analysis also revealed that 10 among the 19 attributes are dominant. These dominant attributes for mineral trapping include calcite, dolomite at injection well, siderite at 200 m away from the injection well, clinochlore, kaolinite, Na + , K + , Ca 2+ , Mg 2+ , pH, and aqeuous CO 2 . Finally, at late times (65–200 years), our results showed that calcite plays a major role in mineral trapping with insignificant contribution from siderite, ankerite, and clay minerals. These findings make the proposed unsupervised ML-model attractive for reactive-transport sensing towards real-time GCS monitoring.

54 ENVIRONMENTAL SCIENCES↗

The effectiveness of D 2 pellet and gas injection in reducing intra-ELM tungsten erosion and heat flux in the DIII-D small angle slot divertor

Edge localized modes (ELMs) in H-mode plasmas can melt and erode plasma-facing components (PFCs) and lead to impurities in the core, reducing confinement. This study analyzes the use of D 2 pellet and gas injection for ELM mitigation on the DIII-D tokamak during the 2022 Small Angle Slot V-shaped Tungsten (W) (SAS-VW) divertor campaign, reducing W erosion and heat flux during ELMs. D α (656 nm) and WI (400.9 nm) filterscopes and Langmuir probes provide photon emission and electron density/temperature to estimate W atom erosion using the S/XB method, while surface eroding thermocouples measured ELM peak heat flux at the outer strike point (OSP). Thomson Scattering measurements of pedestal T e and n e provided input to predict W divertor erosion and heat flux during ELMs via the Free-Streaming plus Recycling Model (FSRM). While a greater D 2 mass injection rate decreased the ELM peak heat flux, the impact on W erosion was not monotonic. The average ‘large’ intra-ELM W erosion was lower for plasma shots with D 2 mass injection compared to the plasma shots without any D 2 mass injection in the SAS-VW divertor. However, plasma shots did not see significant changes in ‘large’ intra-ELM W divertor erosion as the D 2 mass injection rate increased. On average, the FSRM overestimated SAS-VW experimental intra-ELM W erosion by a factor of 6.9, but the overestimation is reduced to a factor of 3.6 with the implication of C deposition effects. Generally, experimentally measured and predicted quantities were worse for plasma shots with a lower D 2 mass injection rate and corresponding higher plasma carbon (C) impurity percentage (f C ). The discrepancies are postulated to be due to C/W material mixing, for which a simple analytic mixed-material model is presented. These results highlight the importance of incorporating and improving the robustness of a mixed-material layer model in the analysis of PFC erosion on present and future tokamak devices.

Carbon (C)↗

A comparative experimental study of IOR potential in fractured shale reservoirs by cyclic water and nitrogen gas injection

Oil production from tight and shale formations accounted for more than half of total U.S. oil production in 2015 (EIA, 2016). Such amount is expected to grow significantly as the active development of low permeability reservoirs continues. Since the primary recovery is becoming less effective and large amounts of oil are locked in matrix, IOR techniques should be considered to face such challenge. Many recent studies have evaluated the potential of gas injection in shale plays. However, few studies discussed the feasibility of water huff-n-puff process. Here this study evaluated the potential of cyclic water injection (CWI) in shale plugs and compared it with the performance of cyclic gas injection (CGI). Eagle Ford core samples were used in this study and saturated with dead shale oil. X-ray diffraction (XRD) technique was employed to analyze the mineral composition of the sample. To reduce clay swelling, 5 wt% of potassium chloride (KCl) solution was used as the injection fluid in CWI tests. Two groups of experiments were performed to examine the effects of soaking time and injection pressure (Pin) on the recovery performance. For one cycle of huff-n-puff, after reaching the Pin, the core plug was soaked with water for a certain time, then the surrounding pressure was released to enter the production period. Under the same operating conditions, CGI tests were conducted to compare the two IOR performances. The CWI experimental results show that soaking time affects the recovery factor (RF) of a single cycle within a certain range, which was similarly concluded from the CGI tests. Injection pressure can affect the recovery performance of CWI significantly. For example, after performing 12 cycles of water huff-n-puff, the RF for tests with Pin of 1000 psi and 5000 psi were 14% and 21%, respectively. When applying 5000 psi, fractures were created on the plug and the size enlarged with further cycles operated. Comparing the two processes of CWI and CGI, different recovery characters can be observed. CWI showed the recovery potential at the first four cycles, and then the incremental oil recovery decreased dramatically in the subsequent cycles. By contrast, CGI presented a more continuous and steady recovery performance with high incremental RF, and then gradually diminished after seven cycles of huff-n-puff processes.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Flow regimes for fluid injection into a confined porous medium

We report theoretical and numerical studies of the flow behaviour when a fluid is injected into a confined porous medium saturated with another fluid of different density and viscosity. For a two-dimensional configuration with point source injection, a nonlinear convection–diffusion equation is derived to describe the time evolution of the fluid–fluid interface. In the early time period, the fluid motion is mainly driven by the buoyancy force and the governing equation is reduced to a nonlinear diffusion equation with a well-known self-similar solution. In the late time period, the fluid flow is mainly driven by the injection, and the governing equation is approximated by a nonlinear hyperbolic equation that determines the global spreading rate; a shock solution is obtained when the injected fluid is more viscous than the displaced fluid, whereas a rarefaction wave solution is found when the injected fluid is less viscous. In the late time period, we also obtain analytical solutions including the diffusive term associated with the buoyancy effects (for an injected fluid with a viscosity higher than or equal to that of the displaced fluid), which provide the structure of the moving front. Numerical simulations of the convection–diffusion equation are performed; the various analytical solutions are verified as appropriate asymptotic limits, and the transition processes between the individual limits are demonstrated.

58 GEOSCIENCES↗

Effect of the Injection Pressure on Enhancing Oil Recovery in Shale Cores during the CO 2 Huff-n-Puff Process When It Is above and below the Minimum Miscibility Pressure

In CO 2 injection, there is a minimum miscibility pressure (MMP) above that CO 2 can be miscible with oil, so that oil recovery will be high. This work is to investigate the effect of the injection pressure on enhanced oil recovery in shale oil cores under huff-n-puff CO 2 injection, when the pressure is above and below the MMP. We first estimated the MMP for a Wolfcamp oil using slimtube tests. The slimtube test results showed that the estimated MMP for the CO 2 –Wolfcamp crude oil system was about 1620 psi at 104 °F. After that, we conducted 15 CO 2 huff-n-puff experiments using three different Wolfcamp shale cores at pressures below and above the MMP. These pressures were 1200, 1600, 1800, 2000, and 2400 psi. Each huff-n-puff test has 7 cycles. The huff-n-puff experiments for three cores showed that, below the MMP, the injection pressure had a significant effect on enhancing oil recovery. Higher than the MMP, the increased pressure further increased the oil recovery until the injection pressure was about 200 psi higher than the MMP. In the extremely low-permeability shale oil cores, additional pressure is needed to push gas into the deeper core to be miscible with the crude oil inside the core. The results indicated that, to have a high oil recovery in shale oil reservoirs during the CO 2 huff-n-puff process, the injection pressure should be higher (at least 200 psi in this case) than the MMP estimated from slimtube tests.

04 OIL SHALES AND TAR SANDS↗

Optimization of Secondary Air Injection in a Wood-Burning Cookstove: An Experimental Study

Nearly 40% of the world's population regularly cooks on inefficient biomass stoves that emit harmful airborne pollutants, such as particulate matter (PM). Secondary air injection can significantly reduce PM mass emissions to mitigate the health and climate impacts associated with biomass cookstoves. However, secondary air injection can also increase the number of ultrafine particles emitted, which may be more harmful to health. This research investigates the effect of secondary air injection on the mass and size distribution of PM emitted during solid biomass combustion. An experimental wood-burning cookstove platform and parametric testing approach are presented to identify and optimize secondary air injection parameters that reduce PM and other harmful pollutants. Size-resolved measurements of PM emissions were collected and analyzed as a function of parametric stove design settings. The results show that PM emissions are highly sensitive to secondary air injection flow rate and velocity. Although increasing turbulent mixing (through increased velocity) can promote more complete combustion, increasing the total flow rate of secondary air may cause localized flame quenching that increases particle emissions. As a result, biomass cookstoves that implement secondary air injection should be carefully optimized and validated to ensure that PM emission reductions are achieved throughout the particle size range.

54 ENVIRONMENTAL SCIENCES↗

The Potential of Stratospheric Aerosol Injection to Reduce the Climatic Risks of Explosive Volcanic Eruptions

Sulfur-rich volcanic eruptions happen sporadically. If Stratospheric Aerosol Injection (SAI) were to be deployed, it is likely that explosive volcanic eruptions would happen during such a deployment. Here we use an ensemble of Earth System Model simulations to show how changing the injection strategy post-eruption could be used to reduce the climate risks of a large volcanic eruption; the risks are also modified even without any change to the strategy. For a medium-size eruption (10 Tg-SO 2 ) comparable to the SAI injection rate, the volcanic-induced cooling would be reduced if it occurs under SAI, especially if artificial sulfur dioxide injections were immediately suspended. Alternatively, suspending injection only in the eruption hemisphere and continuing injection in the opposite would reduce shifts in precipitation in the tropical belt and thus mitigate eruption-induced drought. Finally, we show that for eruptions much larger than the SAI deployment, changes in SAI strategy would have minimal effect.

54 ENVIRONMENTAL SCIENCES↗

Stratigraphy‐Induced Localization of Microseismicity During CO 2 Injection in Illinois Basin

Abstract Subsurface fluid injection stimulates complex hydromechanical interaction, necessitating the integration of geomechanical data across spatial and temporal scales to consider the sophisticated behavior. Induced seismic response is usually associated with the complex reservoir architecture and pre‐existing features that are three‐dimensional, such as local stratigraphy, fractures, faults, and other discontinuities. This study encompasses laboratory characterization of the coupled hydromechanical response of cores extracted from rock formations in Illinois Basin: reservoir ‐ Mt. Simon sandstone, basal seal ‐ Argenta sandstone, and crystalline basement ‐ Precambrian rhyolite. High‐resolution numerical modeling allows considering the three‐dimensional complexity of the Illinois Basin Decatur Project with spatial resolution comparable to one of the active seismic surveys. A detailed reconstruction of the evolving state of stress in formations lacking direct stress measurements is achieved by numerical modeling that integrated laboratory‐derived hydromechanical properties, a porosity‐permeability relationship, active seismic data, and an inverted three‐dimensional porosity distribution. It appears that the microseismic clusters, mainly observed in the crystalline basement during the injection, are linked to zones experiencing more critically stressed conditions prior to injection. These zones have a potential for reactivation during the injection and are attributed to the specific local stratigraphy of the injection site, as well as transfer of triggering perturbations during the injection.

Bondarenko, N. [University of Illinois Urbana‐Cham↗

Injections through skin colonized with Staphylococcus aureus biofilm introduce contamination despite standard antimicrobial preparation procedures

While surgical site preparation has been extensively studied, there is little information about resistance of skin microbiota in the biofilm form to antimicrobial decontamination, and there are no quantitative models to study how biofilm might be transferred into sterile tissue/implant materials during injections for joint spine and tendon, aspiration biopsies and dermal fillers (DF). In this work, we develop two in vitro models to simulate the process of skin preparation and DF injection using pig skin and SimSkin (silicone) materials, respectively. Using the pig skin model, we tested three of the most common skin preparation wipes (alcohol, chlorhexidine and povidone iodine) and found that during wiping they reduced the biofilm bacterial burden of S. aureus (CFU cm-2) by three logs with no statistically significant differences between wipes. Using the SimSkin model, we found that transfer of viable bacteria increased with needle diameter for 30G, 25G and 18G needles. Transfer incidence decreased as injection depth was increased from 1 mm to 3 mm. Serial puncture and linear threading injection styles had similar transfer incidence, whereas fanning significantly increased transfer incidence. The results show that contamination of DF during injection is a risk that can be reduced by modifying skin prep and injection practices.

60 APPLIED LIFE SCIENCES↗