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NASA NTRS1986

Computer simulation of two electrophoretic columns coupled for isoelectric focusing in simple buffers

Computer simulation is used to analyze a system of two electrophoretic columns coupled by mixing the anolyte of one with the catholyte of the other. A mathematical model is presented which is used to predict the pH gradients formed by monovalent buffers in this system, when the currents in the columns are unequal. In the column with the higher current a pH gradient is created which increases from anode to cathode and is potentially useful for isoelectric focusing. The breadth of this gradient is dependent upon the ratio of the currents. The function of the second column is the compensation of buffer migration which occurs in the first column, thereby maintaining constant electrolyte composition. The effects of buffer pKs and mobilities are evaluated.

Tsai, Amos↗

NASA NTRS1985

The formation of stable pH gradients with weak monovalent buffers for isoelectric focusing in free solution

Two methods which utilize simple buffers for the generation of stable pH gradients (useful for preparative isoelectric focusing) are compared and contrasted. The first employs preformed gradients comprised of two simple buffers in density-stabilized free solution. The second method utilizes neutral membranes to isolate electrolyte reservoirs of constant composition from the separation column. It is shown by computer simulation that steady-state gradients can be formed at any pH range with any number of components in such a system.

Mosher, Richard A.↗

NASA NTRS1986

Scale-up of isoelectric focusing

The paper describes some applications to large scale protein fractionation using a recycling isoelectric focusing apparatus. Separation is achieved in free solution without the use of supporting media. Various alternatives for the formation of the pH gradient are discussed and results of a computer simulation are presented.

Bier, Milan↗

NASA NTRS1987

Isoelectric focusing-affinity immunoblot analysis of mouse monoclonal antibodies to the four human IgG subclasses

Isoelectric focusing (IEF)/affinity immunoblotting and enzyme-linked immunosorbent assay (ELISA) were used for parallel analysis of murine monoclonal antihuman IgG-subclass antisera (MoAbs). Coomassie Blue-stained protein bands in the pH region 5.5-8.0 were shown to be murine IgG by direct blotting onto nitrocellulose followed by detection with conjugated antimouse IgG. Use of IgG myeloma antigen-coated nitrocellulose in the IEF-affinity immunoblot allowed detection of the charge microheterogeneity of MoAbs. The MoAb group contained one to five major dense bands flanked by up to four minor fainter bands, all with pIs ranging from 6.1 to 7.8. Semiquantitative estimates of binding specificity in the IEF-affinity blot compared well with cross-reactivity data obtained from a quantitative ELISA.

Hamilton, Robert G.↗

NASA NTRS1986

Affinity immunoblotting - High resolution isoelectric focusing analysis of antibody clonotype distribution

A sensitive and specific method is proposed for the analysis of specific antibody clonotype changes occurring during an immune response and for comparing multiple sera for antibody clonotype similarities. Polyclonal serum antibodies separated by isoelectric focusing (IEF) were analyzed by an affinity immunoblotting method using antigen-coated nitrocellulose membranes. Antibodies present on the surface of the acrylamide gels following IEF bind the antigen on the nitrocellulose when the coated nitrocellulose is laid over the gels. The technique has been used to analyze Ig clonotypes specific for five protein antigens and two carbohydrate antigens. Optimal antigen concentrations for coating the nitrocellulose membranes were found to range from 10-100 microgram/ml.

Knisley, Keith A.↗

NASA NTRS1987

Statistical treatment of fluctuations in the gravitational focusing of light due to stellar masses within a gravitational lens

When light from small, distant sources in the universe is gravitationally focused by an intervening galaxy, the gravitational lens can be influenced by the granularity of the matter distribution which is caused by the stellar (or other compact) masses in the galaxy. A largely analytic, statistical calculation for a gravitational lens due to a collection of compact masses - valid for sources of finite size and for large (as well as small) 'optical depths' for the lens - is developed to treat fluctuations in the light caused by such 'microfocusing' effects. Previous treatments have been either numerical simulations of the Monte Carlo type or limited to single-star (i.e., low-optical-depth) effects.

Deguchi, Shuji↗

NASA NTRS1985

Characterization of synthetic carrier ampholytes by repetitive scanning of the electric field during focusing

This paper reports the utilization of a potential gradient array detector for monitoring the dynamics of the electric field during isoelectric focusing. Transient and steady state electric field profiles are presented for synthetic carrier ampholyte mixtures with a wide (about 3-10) pH range. Two available commercial products (Ampholine and Pharmalyte) and a laboratory synthesized mixture (PEHA ampholytes) are compared. The formation of conductivity gaps and their migration toward the cathode in extended experiments (cathodic drift) can be visualized with this system.

Thormann, W.↗

NASA NTRS1988

Space Station - A focus for the development of structural dynamics scale model technology for large flexible space structures

The paper discusses the scale model technology being developed at the NASA Langley Research Center in support of the development of structural dynamic prediction methods for large flexible space structures. Space Station is used as a focus. The Dynamic Scale Model Technology (DSMT) Program is described; results of the effect of scaling Space Station components are presented; and supporting scale model technology development activities are described and results presented. The conceptual design of a hybrid-scale Pathfinder model for developing test techniques and suspension methods is also discussed, and a summary of the broad application of the scale model technology being developed is presented.

Letchworth, Robert↗

NASA NTRS1988

GaAs/GaAlAs distributed Bragg reflector laser with a focused ion beam, low dose dopant implanted grating

The performance of a GaAs/GaAlAs distributed Bragg reflector (DBR) laser using a focused ion beam implanted grating (FIB-DBR) is reported for the first time. Stripes of Si(2+) with a period of 2300 A and a dose about 10 to the 14th/sq cm are directly implanted into the passive large optical cavity layer to provide the distributed feedback. Surface-emitting light from the second-order grating is observed. Threshold current of 110 mA and single DBR mode operation from 20 to 40 C are obtained. The wavelength tuning rate with temperature is 0.8 A/C. The coupling coefficient is estimated to be 15/cm. The results show that FIB technology is practical for distributed feedback and DBR lasers and optoelectronic integrated circuits.

Wu, M. C.↗

NASA NTRS1988

Ion-beam focusing in a double-plasma device

The authors studied the propagation of a low-energy charge-neutralized ion beam injected into the target region of a long double-plasma device. A magnetic field of up to about 180 G may be applied along the axis of the device. As a result of charge exchange collisions, the ion beam is attenuated as it propagates into the target region. However, under certain conditions of magnetic field strength and neutral gas pressure, the authors have observed a `reemergence' of the beam on axis far downstream in the target. This reemergence of the ion beam is attributed to a focusing of the ions by a self-consistently produced radial ambipolar electric field. The effect may be expected to occur in other types of plasma devices as well, whenever a sufficiently large radially inward electric field is present.

Johnson, James C.↗

NASA NTRS1988

Focus drive mechanism for the International Ultraviolet Explorer

The mechanical design of the compact and robust focus drive apparatus developed for the IUE's scientific instrumentation is based on a novel planetary drive that converts the angular increments of a permanent magnet stepping motor into simultaneous linear displacements of three ball screws supporting the secondary mirror. Significant torque margins are thus produced while yielding exceptionally smooth and reliable operation. Attention is given to mechanical drive materials-selection criteria.

Schuster, Ludwig E.↗

NASA NTRS1988

Theory for synthetic aperture radar imaging of the ocean surface - With application to the Tower Ocean Wave and Radar Dependence experiment on focus, resolution, and wave height spectra

A one-dimensional model for simulating azimuthal SAR imaging of the ocean surface is developed which can admit both the 'distributed surface' and 'velocity bunching' approaches. Computer simulations demonstrate that the time-dependent modulation patterns due to the radar cross section variation and the velocity bunching effects provide optimum focusing around half the phase velocity of the long wave. The results indicate that in the Tower Ocean Wave and Radar Dependence experiment, SAR imaging at L band is approximately linear.

Kasilingam, Dayalan P.↗

NASA NTRS1988

Isoelectric focusing analysis of antibody clonotype changes occurring during immune responses using immobilized pH gradients

Serum was collected from rabbits at 2-day intervals following a single injection with tetanus toxoid or at weekly intervals following multiple injections with Micrococcus lysodeikticus cell walls. These sera were analyzed for the presence of individual clonotypes of specific antitetanus or antimicrococcal antibodies by isoelectric focusing in immobilized pH gradients with added carrier ampholytes followed by affinity immunoblotting. The affinity immunoblots obtained clearly defined both the rapid disappearance and late appearance of distinct subsets of antibody clonotypes during the response. These data demonstrate the application of affinity immunoblotting combined with immobilized pH gradients for detecting the subtle changes in specific antibody clonotype patterns which occur during an immune response.

Knisley, Keith A.↗

NASA NTRS1990

Borescope With Large Depth Of Focus

Modification of commercial borescope yields clear, glare-free images of defects on inside of tube. Used to examine diverging wall of tube. Wall illuminated by light from fiber distinct from fused-fiber cable used for viewing. Viewing cable holds right-angle mirror at tip so it can look sideways. Image appears, magnified, on monitor. Instrument offers large depth of focus and therefore used in tubes of varying inside diameter.

Guirguis, Kamal S.↗

NASA NTRS1989

Near-planar TS waves and longitudinal vortices in channel flow: Nonlinear interaction and focusing

The nonlinear interaction between planar or near-planar Tollmien-Schlichting waves and longitudinal vortices, induced or input, is considered theoretically for channel flows at high Reynolds numbers. Several kinds of nonlinear interaction, dependent on the input amplitudes and wavenumbers or on previously occurring interactions, are found and inter-related. The first, Type 1, is studied the most here and it usually produces spanwise focusing of both the wave and the vortex motion, within a finite scaled time, along with enhancement of both their amplitudes. This then points to the nonlinear interaction Type 2 where new interactive effects come into force to drive the wave and the vortex nonlinearly. Types 3, 4 correspond to still higher amplitudes, with 3 being related to 2, while 4 is connected with a larger-scale interaction 5 studied in an allied paper. Both 3, 4 are subsets of the full three-dimensional triple-deck-lie interaction, 6. The strongest nonlinear interactions are those of 4, 5, 6 since they alter the mean-flow profile substantially, i.e., by an 0(1) relative amount. All the types of nonlinear interaction however can result in the formation of focussed responses in the sense of spanwise concentrations and/or amplifications of vorticity and wave amplitude.

Hall, P.↗

NASA NTRS1989

Focus of attention in an activity-based scheduler

Earlier research in job shop scheduling has demonstrated the advantages of opportunistically combining order-based and resource-based scheduling techniques. An even more flexible approach is investigated where each activity is considered a decision point by itself. Heuristics to opportunistically select the next decision point on which to focus attention (i.e., variable ordering heuristics) and the next decision to be tried at this point (i.e., value ordering heuristics) are described that probabilistically account for both activity precedence and resource requirement interactions. Preliminary experimental results indicate that the variable ordering heuristic greatly increases search efficiency. While least constraining value ordering heuristics have been advocated in the literature, the experimental results suggest that other value ordering heuristics combined with our variable-ordering heuristic can produce much better schedules without significantly increasing search.

Sadeh, Norman↗

NASA NTRS1989

Assembly, alignment, and cold focus test methods utilized on CLAES optics

This paper describes the techniques and the equipment used in the assembly and alignment of the Cryogenic Limb Array Etalon Spectrophotometer (CLAES) Telescope and Spectrometer, together with those used to test the system at operating temperatures in order to establish the correct focus for the detector array and other system components. In addition, cryogenic tests were used to determine the stability of the optics over the specified environment, and blur size measurements were performed at operational temperatures.

Dawson, Juan C.↗

NASA NTRS1989

Electrophoresis and isoelectric focusing of whole cell and membrane proteins from the extremely halophilic archaebacteria

The subunits from two purified halobacterial membrane enzymes (ATPase and nitrate reductase) behaved differently with respect to isoelectric focusing, silver staining and interaction with ampholytes. Differential behavior was also observed in whole cell proteins from Halobacterium saccharovorum regarding resolution in two-dimensional gels and silver staining. It is proposed that these differences reflect the existence of two classes of halobacterial proteins.

Stan-Lotter, Helga↗