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At least 361 records · Page 20

View angle effect in LANDSAT imagery

The view angle effect in LANDSAT 2 imagery was investigated. The LANDSAT multispectral scanner scans over a range of view angles of -5.78 to 5.78 degrees. The view angle effect, which is caused by differing view angles, could be studied by comparing data collected at different view angles over a fixed location at a fixed time. Since such LANDSAT data is not available, consecutive day acquisition data were used as a substitute: they were collected over the same geographical location, acquired 24 hours apart, with a view angle change of 7 to 8 degrees at a latitude of 35 to 45 degrees. It is shown that there is approximately a 5% reduction in the average sensor response on the second-day acquisitions as compared with the first-day acquisitions, and that the view angle effect differs field to field and crop to crop. On false infrared color pictures the view angle effect causes changes primarily in brightness and to a lesser degree in color (hue and saturation). An implication is that caution must be taken when images with different view angles are combined for classification and a signature extension technique needs to take the view angle effect into account.

Kaneko, T.↗

Dynamic Coupling of Quasi-Electrostatic Thundercloud Fields to the Mesosphere and Lower Ionosphere: Sprites and Jets

Red Sprites and Blue Jets are two different types of recently discovered optical flashes ob- served above large thunderstorm systems. Sprites are luminous glows occurring at altitudes typically ranging from approximately 50 to 90 km. In video they exhibit a red color at their top which gradually changes to blue at lower altitudes. Sprites may occur singly or in clusters of two or more. The lateral extent of "unit" sprites is typically 5-10 km and they endure for several milliseconds. Jets are upward moving (approximately 100 km/s) highly collimated beams of luminosity, emanating from the tops of thunderclouds, extending up to approximately 50 km altitude and exhibiting a primarily blue color. We propose that sprites result from large electric field transients capable of causing electron heating, breakdown ionization and excitation of optical emissions at mesospheric altitudes following the removal of thundercloud charge by a cloud-to-ground discharge. Depending on the history of charge accumulation and removal, and the distribution of ambient atmospheric conductivity, the breakdown region may have the shape of vertically oriented ionization column(s). Results of a two-dimensional and self consistent quasi-electrostatic (QE) model indicate that most of the observed features of sprites can be explained in terms of the formation and self-driven propagation of streamer type channels of breakdown ionization. Comparison of the optical emission intensities of the 1st and 2nd positive bands of N2, Meinel and 1st negative bands of N2(+) and the 1st negative band of O2(+) demonstrates that the 1st positive band of N2 is the dominant optical emission in the altitude range approximately 50-90 km, which accounts for the observed red color of sprites. Optical emissions of the 1st and 2nd positive bands of N2 occur in carrot-like vertical structures with typical transverse dimension approximately 5-10 km which can span an altitude range from approximately 80 km to well below approximately 50 km. The appearance of optical emissions associated with sprites can be delayed in time (approximately 1-20 ms) with respect to the causative cloud to ground discharge. Theoretical model results are found to be in good agreement with recent video, photometric and spectral measurements of sprites.

Pasko, Victor Petrovich↗

Comparison of DNA fragmentation and color thresholding for objective quantitation of apoptotic cells

Apoptosis is a process of cell death characterized by distinctive morphological changes and fragmentation of cellular DNA. Using video imaging and color thresholding techniques, we objectively quantitated the number of cultured CD4+ T-lymphoblastoid cells (HUT78 cells, RH9 subclone) displaying morphological signs of apoptosis before and after exposure to gamma-irradiation. The numbers of apoptotic cells measured by objective video imaging techniques were compared to numbers of apoptotic cells measured in the same samples by sensitive apoptotic assays that quantitate DNA fragmentation. DNA fragmentation assays gave consistently higher values compared with the video imaging assays that measured morphological changes associated with apoptosis. These results suggest that substantial DNA fragmentation can precede or occur in the absence of the morphological changes which are associated with apoptosis in gamma-irradiated RH9 cells.

Non-NASA Center↗

Chromophore carbonyl twisting in fluorescent biosensors encodes direct readout of protein conformations with multicolor switching

Fluorescent labeling of proteins is a powerful tool for probing structure-function relationships with many biosensing applications. Structure-based rules for systematically designing fluorescent biosensors require understanding ligand-mediated fluorescent response mechanisms which can be challenging to establish. We installed thiol-reactive derivatives of the naphthalene-based fluorophore Prodan into bacterial periplasmic glucose-binding proteins. Glucose binding elicited paired color exchanges in the excited and ground states of these conjugates. X-ray structures and mutagenesis studies established that glucose-mediated color switching arises from steric interactions that couple protein conformational changes to twisting of the Prodan carbonyl relative to its naphthalene plane. Mutations of residues contacting the carbonyl can optimize color switching by altering fluorophore conformational equilibria in the apo and glucose-bound proteins. A commonly accepted view is that Prodan derivatives report on protein conformations via solvatochromic effects due to changes in the dielectric of their local environment. Here we show that instead Prodan carbonyl twisting controls color switching. These insights enable structure-based biosensor design by coupling ligand-mediated protein conformational changes to internal chromophore twists through specific steric interactions between fluorophore and protein.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Puck Loop Seal (Final Project Technical Report)

Sandia National Laboratories (SNL) is advancing technical capabilities used in passive loop seals. The “Puck” seal used a set of International Atomic Energy Agency (IAEA) requirements for new passive loop seals published in 2020 as a design guide. The seal is based on an oxygen-sensitive inner mixture encased in an oxygen-impermeable shell, is monolithic rather than two-part, incorporates self-capturing wire features, contains colored water beads and bubbles formed during processing as unique identifiers (UIDs), and visually indicates tamper (whether from seal body penetration or from seal wire removal) by irreversibly changing the seal body from multi-colored to black. This paper will provide details on the design, development, and testing of Puck seal prototypes.

42 ENGINEERING↗

Variations in the abundance of transiently heated particles within nearby molecular clouds

IRAS images of molecular clouds in the Chamaeleon, Taurus, and Ursa Major complexes show that the mid-IR emission from transiently heated particles is distributed very differently from the 100 micron emission from large dust grains. The ratio between 12 and 100 micron emission varies by more than one order of magnitude in each complex from about 5 times to about one-quarter of the average value in the solar neighborhood. Within a complex, color variations are seen on all scales. No significant variations of the I(v)(100 micron)/A(v) ratio are observed between clouds of widely different mid- to far-IR color. It is shown that neither the large amplitude of the color variations nor their morphology can be explained by changes of the excitation by the UV radiation field and it is concluded that the color variations trace variations in the abundance of transiently heated particles. A scenario is proposed which relates the abundance variations to the cycling of interstellar matter between the gas-phase and grain surfaces.

Boulanger, F.↗

Investigation of environmental change pattern in Japan: A study on change detection of land cover in Tokyo districts using multi-dates LANDSAT CCT

The author has identified the following significant results. The software program, which enables the geographically corrected LANDSAT digital data base, was developed. The data base could provide land use planners with land cover information and the environmental change pattern. Land cover was evaluated by the color representation for ratio of three primary components, water vegetation, and nonorganic matter. Software was also developed for the change detection within multidates LANDSAT MSS data.

Maruyasu, T.↗

Sensitivity of Ocean Reflectance Inversion Models for Identifying and Discriminating Between Phytoplankton Functional Groups

The daily, synoptic images provided by satellite ocean color instruments provide viable data streams for observing changes in the biogeochemistrY of marine ecosystems. Ocean reflectance inversion models (ORMs) provide a common mechanism for inverting the "color" of the water observed a satellite into marine inherent optical properties (lOPs) through a combination of empiricism and radiative transfer theory. lOPs, namely the spectral absorption and scattering characteristics of ocean water and its dissolved and particulate constituents, describe the contents of the upper ocean, information critical for furthering scientific understanding of biogeochemical oceanic processes. Many recent studies inferred marine particle sizes and discriminated between phytoplankton functional groups using remotely-sensed lOPs. While all demonstrated the viability of their approaches, few described the vertical distributions of the water column constituents under consideration and, thus, failed to report the biophysical conditions under which their model performed (e.g., the depth and thickness of the phytoplankton bloom(s)). We developed an ORM to remotely identifY Noctiluca miliaris and other phytoplankton functional types using satellite ocean color data records collected in the northern Arabian Sea. Here, we present results from analyses designed to evaluate the applicability and sensitivity of the ORM to varied biophysical conditions. Specifically, we: (1) synthesized a series of vertical profiles of spectral inherent optical properties that represent a wide variety of bio-optical conditions for the northern Arabian Sea under aN Miliaris bloom; (2) generated spectral remote-sensing reflectances from these profiles using Hydrolight; and, (3) applied the ORM to the synthesized reflectances to estimate the relative concentrations of diatoms and N Miliaris for each example. By comparing the estimates from the inversion model to those from synthesized vertical profiles, we were able to identifY those bio-optical conditions under which the inversion model performs both well and poorly.

Werdell, P. Jeremy↗

Color Choice is Everything - Impacts Color makes to the Lighting Environment

When contracts are let out to design multiple systems in a vehicle, it is a challenge to maintain integration between system leads. Designers on niche systems, like lighting and control panel design, often get caught up in the challenge of designing the light source or visual interface and fail to include time in their schedule to work with system architects on how their lighting system will be integrated. Additionally, behavioral scientists, industrial designers, and materials engineers get caught up with the materials and look of the system, but often fail to consider how the selection of their materials could affect the certification or performance of electronic devices like lighting systems. Additionally, computer modeling of the system architecture often assumes a perfect environment without the clutter of actual human use (dirt, stowage, crowding). As a result, lighting systems, and backlit displays run the risk of being overdesigned or under designed. Engineers making the assumption that because they have no input or there is no requirement on work surface reflectance, make the assumption that they can t count on good material choices and thus may install more lighting than is necessary. While having more lights may seem better, for a vehicle that is trying to conserve power, more lights may not be a good option. On the other hand, designers who made the opposite assumption and designed a lighting system that only produced just enough light, often wind up with a system that did conserve power, but didn t produce enough light. These situations are exasperated when the system starts to be used and the models are not perfect anymore. The lack of coordination and iterative design not only can impact lighting levels within an environment, but also can affect color perception. This is because, if materials do not represent a gradation of white or black, the material unevenly absorbs and reflects light at different wavelengths of the visual spectrum. The lighting designer may have built a light that meets light spectra requirements, but the eventual light reaching the human user may not be the spectra of light architects intended, if materials near the light source change the spectrum just by how much color is absorbed or reflected. With the recent findings concerning Circadian rhythm, where the spectra of light is extremely important for addressing crew sleep and wake cycles, system architects should pay considerable attention on the impact material choices have in changing the light spectrum in an environment. This presentation will show examples of how material choices impact the resulting illuminance, color spectrum, and power usage of an illuminated space. Its goal is to encourage system designers and planners to use more care in development of requirements and the verification of systems intended for the human visual interface.

Clark, Toni A.↗

Long-term changes in reflectivity and larger scale motions in the atmospheres of Jupiter and Saturn

A multi-color, broad-band photographic program for monitoring atmospheric variability of Jupiter and Saturn with the 61-cm, f/75 telescope was continued. The archivial product consists of approximately 20 sequential images on 3 1/4 x 4 1/4 glass plates with a plate scale of 4.53 arc sec/mm. An eleven-step sensitometric wedge, recorded times of acquisition, and fiducial marks which determine the orientation of the plate, are recorded on each individual plate. This allows accurate positional measurements, as well as detailed relative surface brightness determinations. Detailed measurements of the Red Spot are being utilized in a study of zonal velocity variation and the ability to predict the longitude of the Red Spot during the Galileo mission. An ongoing 5-color series of Saturn has been maintained to map the seasonal changes in the belt-zone reflectivity. Digitization of a series of blue images containing the Red Spot and a series of red and blue images excluding the Red Spot are being processed and reduced to normalized surface brightness maps. This data is being utilized to map time-dependent brightness variations of selected features, belts, and ones.

Beebe, R. F.↗

Imaging Technique for High Precision Measurement of Detector Stability for the Ocean Color Instrument

One of the new projects by NASA to explore environmental change is the Plankton, Aerosol, Cloud, ocean Ecosystem (PACE) spacecraft. The primary instrument aboard the PACE spacecraft is the Ocean Color Instrument (OCI), an advanced optical spectrometer that will measure the color of the ocean from the ultraviolet to shortwave infrared range for the purpose of observing and monitoring ocean and coastal biology. As part of the optical alignment testing that must be completed before the instrument is built, the measurement stability of the detectors at space flight operating temperatures must be determined to ensure alignment is maintained. Therefore, this project focuses on the development of an imaging technique that adheres to testing requirements and can be used to determine the stability of the OCI detector. The movements of targets affixed to a high-precision stage were analyzed through an image processing program to test the ability of the imaging technique to detect very small (under 10 micrometers) movements. It was found that movements as small as half a pixel (~6 microns) and 1 pixel (~12 microns) were able to be accurately and consistently detected with this imaging technique. This project found that the designed image analysis technique fulfills testing and measurement requirements and will be an effective metrology technique for the OCI detector stability test.

Optics↗

Autonomously Responsive Membranes for Chemical Warfare Protection

Stimuli-responsive materials offer new opportunities to resolve long-standing material challenges and are rapidly gaining pivotal roles in diverse applications. For example, smart protective garments that rapidly transport water vapor and autonomously block chemical threats are expected to enable an effective new paradigm of adaptive personal protection. However, the incorporation of these seemingly incompatible properties into a single responsive system remains elusive. Herein, a bistable membrane that can rapidly, selectively, and reversibly transition from a highly breathable state in a safe environment to a chemically protective state when exposed to organophosphate threats such as sarin is demonstrated. Dynamic response to chemical stimuli is achieved through the physical collapse of an ultrathin copolymer layer on the membrane surface, which efficiently gates transport through membrane pores composed of single-walled carbon nanotubes (SWNTs). The adoption of nanometer-wide SWNTs for ultrafast moisture conduction enables a simultaneous boost in size-sieving selectivity and water-vapor permeability by decreasing nanotube diameter, thereby overcoming the breathability/protection trade-off that limits conventional membrane materials. Adaptive multifunctional membranes based on this platform greatly extend the active use of a protective garment and present exciting opportunities in many other areas including separation processes, sensing, and smart delivery.

36 MATERIALS SCIENCE↗

Shortened processing time technique for color industrial radiography

Improved processing method reduces time required to generate a color radiograph. Prior to, or after exposure to penetrating radiation, the emulsion side of the film is flashed to a colored light which produces the hue changes in the processed radiograph. Agitation of the film during processing assures uniformity of results.

Lapinski, N. P.↗

Fracture trends identified by ERTS-1 imagery in Utah and Nevada

The author has identified the following significant results. Structures clearly traceable through bed rock are considered most reliable. Continuity, however, is frequently traceable through ranges and across adjoining sedimentary basins where changes in drainage, erosion pattern, soil color, or vegetation suggest that the basin sediments are extremely sensitive to underlying structures. Many large and continuous trends, too smeared by erosion to be visible on the ground or from aircraft photos, become quite evident at the scale of the ERTS-1 images. The study was made with little regard for surface geology. The trends have been retraced to separate and unidirectional sets. These are now being related to surface geology, geophysical studies, volcanic and intrusive centers, and areas of mineralization.

Jensen, M. L.↗

GaN for LED applications

In order to improve the synthesis of GaN the effect of various growth and doping parameters has been studied. Although Be, Li, Mg, and Dy can be used to overcompensate native donors, the most interesting acceptor element is Zn. The emission spectrum and the luminescence efficiency depend on the growth temperature (below 800 C), on the partial pressure of the doping impurity, and on the duration of growth. Blue-green electroluminescence with a power efficiency of 0.1 percent and a brightness of 850 fL (at 0.6 mA and 22.5 V) was obtained. Some diodes allow the color of the emitted light to change by reversing the polarity of the bias. Continuous operation of a diode over a period of 5 months showed no evidence of degradation. The luminescence properties of ion-implanted GaN were studied. Delay effects were found in the electroluminescence of diodes, although, with a dc bias, a 70-MHz modulation was possible.

Pankove, J. I.↗

Quaternary geologic map of Minnesota

The Quaternary Geologic Map of Minnesota is a compilation based both on the unique characteristics of satellite imagery and on the results of previous field investigations, both published and unpublished. The use of satellite imagery has made possible the timely and economical construction of this map. LANDSAT imagery interpretation proved more useful than expected. Most of the geologic units could be identified by extrapolating from specific sites where the geology had been investigated into areas where little was known. The excellent geographic registry coupled with the multi-spectral record of these images served to identify places where the geologic materials responded to their ecological environment and where the ecology responded to the geologic materials. Units were well located on the map at the scale selected for the study. Contacts between till units could be placed with reasonable accuracy. The reference points that were used to project delineations between units (rivers, lakes, hills, roads and other features), which had not been accurately located on early maps, could be accurately located with the help of the imagery. The tonal and color contrasts, the patterns reflecting geologic change and the resolution of the images permitted focusing attention on features which could be represented at the final scale of the map without distraction by other interesting but site-specific details.

Goebel, J. E.↗