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At least 361 records · Page 20

B-DNA to Z-DNA structural transitions in the SV40 enhancer: stabilization of Z-DNA in negatively supercoiled DNA minicircles

During replication and transcription, the SV40 control region is subjected to significant levels of DNA unwinding. There are three, alternating purine-pyrimidine tracts within this region that can adopt the Z-DNA conformation in response to negative superhelix density: a single copy of ACACACAT and two copies of ATGCATGC. Since the control region is essential for both efficient transcription and replication, B-DNA to Z-DNA transitions in these vital sequence tracts may have significant biological consequences. We have synthesized DNA minicircles to detect B-DNA to Z-DNA transitions in the SV40 enhancer, and to determine the negative superhelix density required to stabilize the Z-DNA. A variety of DNA sequences, including the entire SV40 enhancer and the two segments of the enhancer with alternating purine-pyrimidine tracts, were incorporated into topologically relaxed minicircles. Negative supercoils were generated, and the resulting topoisomers were resolved by electrophoresis. Using an anti-Z-DNA Fab and an electrophoretic mobility shift assay, Z-DNA was detected in the enhancer-containing minicircles at a superhelix density of -0.05. Fab saturation binding experiments demonstrated that three, independent Z-DNA tracts were stabilized in the supercoiled minicircles. Two other minicircles, each with one of the two alternating purine-pyrimidine tracts, also contained single Z-DNA sites. These results confirm the identities of the Z-DNA-forming sequences within the control region. Moreover, the B-DNA to Z-DNA transitions were detected at superhelix densities observed during normal replication and transcription processes in the SV40 life cycle.

Non-NASA Center↗

Comparative Analysis of Interference Pathloss Coupling Patterns on B-737 VS. B757 Airplanes

Portable wireless technology provides many benefits to modern day travelers. Over the years however, numerous reports have cited portable electronic devices (PEDs) as a possible cause of electromagnetic interference (EMI) to aircraft navigation and communication radio systems. PEDs may act as transmitters, both intentional and unintentional, and their signals may be detected by the various radio receiver antennas installed on the aircraft. Measurement of the radiated field coupling between passenger cabin locations and aircraft communication and navigation receivers, via their antennas is defined herein as interference path loss (IPL). IPL data is required for assessing the threat of PEDs to aircraft radios, and is very dependent upon airplane size, the interfering transmitter position within the airplane, and the location of the particular antenna for the aircraft system of concern. NASA Langley Research Center, Eagles Wings Inc., and United Airlines personnel performed extensive IPL measurements on several Boeing 737 airplanes. In the Spring of 2004, extensive IPL measurements were also taken on several Boeing 757 airplanes under a cooperative agreement between NASA Langley Research Center and Delta Airlines. The objective of this paper is to analyze IPL measurement data, to better understand the impact on coupling levels based on the different locations of the aircraft radio antennas on B-757 and B-737 airplanes, and to provide a basis for future fuzzy logic modeling of airplane IPL. This effort will build upon previous fuzzy modeling of IPL data for B-737 airplane data.

Jafri, Madiha↗

Proposed Facility Modifications to Support Propulsion Systems Testing Under Simulated Space Conditions at Plum Brook Station's Spacecraft Propulsion Research Facility (B-2)

Preparing NASA's Plum Brook Station's Spacecraft Propulsion Research Facility (B-2) to support NASA's new generation of launch vehicles has raised many challenges for B-2 s support staff. The facility provides a unique capability to test chemical propulsion systems/vehicles while simulating space thermal and vacuum environments. Designed and constructed 4 decades ago to support upper stage cryogenic engine/vehicle system development, the Plum Brook Station B-2 facility will require modifications to support the larger, more powerful, and more advanced engine systems for the next generation of vehicles leaving earth's orbit. Engine design improvements over the years have included large area expansion ratio nozzles, greater combustion chamber pressures, and advanced materials. Consequently, it has become necessary to determine what facility changes are required and how the facility can be adapted to support varying customers and their specific test needs. Instrumental in this task is understanding the present facility capabilities and identifying what reasonable changes can be implemented. A variety of approaches and analytical tools are being employed to gain this understanding. This paper discusses some of the challenges in applying these tools to this project and expected facility configuration to support the varying customer needs.

Edwards, Daryl A.↗

Airborne Observations of the Spatial and Temporal Variability of Tropospheric Carbon Dioxide during the INTEX-B Campaign

The Intercontinental Chemical Transport Experiment-North America (INTEX-NA) is an international field campaign envisioned to investigate the transport and transformation of gases and aerosols on transcontinental/intercontinental scales and assess their impact on air quality and climate. Phase B (INTEX-B) of the mission was conducted during a 10- week period from March 1 to May 15, 2006 and focused initially on pollution outflow from the Mexico City Metropolitan Area, later addressing the transport of pollution from Asia to North America during springtime meteorological conditions. During the deployment, fast-response (1-s resolution) CO2 measurements were recorded aboard the NASA DC-8 providing valuable regional-scale information on carbon sources and sinks over sparsely sampled areas of North America and adjacent ocean basins. When coupled with the enormously sophisticated chemistry payload on the DC-8, these measurements collectively afford extremely powerful multi-tracer constraints for carbon source/sink attribution. Preliminary examination of the two data sets from the INTEX-B campaign, acquired one month apart, reveals not only the influence of the CO2 seasonal cycle, but also the preponderance of human population and industrial activity in the northern hemisphere. In this presentation, a synergy of the ensemble of airborne and surface observations, bottomup emission inventories, as well as transport history are invoked in a GIS framework to elucidate the source/sink processes reflected in the observations. The airborne CO2 data, along with simultaneous surface measurements (e.g. NOAA ESRL), are examined to establish the vertical distribution and variability of CO2 as a function of location. The role of localized sources, long-range transport, the biosphere, stratospheric exchange, and dynamical processes on the CO2 spatial variability observed throughout the tropospheric column will be discussed.

Vay, Stephanie A.↗

Quantitative Studies of the Optical and UV Spectra of Galactic Early B Supergiants

We undertake an optical and ultraviolet spectroscopic analysis of a sample of 20 Galactic B0-B5 supergiants of luminosity classes Ia, Ib, Iab, and II. Fundamental stellar parameters are obtained from optical diagnostics and a critical comparison of the model predictions to observed UV spectral features is made. Methods. Fundamental parameters (e.g., T(sub eff), log L(sub *), mass-loss rates and CNO abundances) are derived for individual stars using CMFGEN, a nLTE, line-blanketed model atmosphere code. The impact of these newly derived parameters on the Galactic B supergiant Ten scale, mass discrepancy, and wind-momentum luminosity relation is examined. Results. The B supergiant temperature scale derived here shows a reduction of about 1000-3000 K compared to previous results using unblanketed codes. Mass-loss rate estimates are in good agreement with predicted theoretical values, and all of the 20 BO-B5 supergiants analysed show evidence of CNO processing. A mass discrepancy still exists between spectroscopic and evolutionary masses, with the largest discrepancy occuring at log (L/(solar)L approx. 5.4. The observed WLR values calculated for B0-B0.7 supergiants are higher than predicted values, whereas the reverse is true for B1-B5 supergiants. This means that the discrepancy between observed and theoretical values cannot be resolved by adopting clumped (i.e., lower) mass-loss rates as for O stars. The most surprising result is that, although CMFGEN succeeds in reproducing the optical stellar spectrum accurately, it fails to precisely reproduce key UV diagnostics, such as the N v and C IV P Cygni profiles. This problem arises because the models are not ionised enough and fail to reproduce the full extent of the observed absorption trough of the P Cygni profiles. Conclusions. Newly-derived fundamental parameters for early B supergiants are in good agreement with similar work in the field. The most significant discovery, however, is the failure of CMFGEN to predict the correct ionisation fraction for some ions. Such findings add further support to revising the current standard model of massive star winds, as our understanding of these winds is incomplete without a precise knowledge of the ionisation structure and distribution of clumping in the wind. Key words. techniques: spectroscopic - stars: mass-loss - stars: supergiants - stars: abundances - stars: atmospheres - stars: fundamental parameters

Searle, S. C.↗

Discovery of a New Dusty B[E] Star in the Small Magellanic Cloud

We present new optical spectroscopic and Spitzer IRAC photometric observations of a B-type star in the SMC cluster NGC 346, NGC 346:KWBBe 200. We detect numerous Fe II, [O I], [Fe II], as well as strong P-Cygni profile H I emission lines in its optical spectrum. The star's near-IR color and optical to IR SED clearly indicate the presence of an infrared excess, consistent with the presence of gas and warm, T -800 K, circumstellar dust. Based on a crude estimate of the star's luminosity and the observed spectroscopic line profile morphologies, we find that the star is likely to be a B-type supergiant. We suggest that NGC 346:KWBBe 200 is a newly discovered B[e] supergiant star, and represents the fifth such object to be identified in the SMC.

Wisniewski, John P.↗

Proposed Facility Modifications to Support Propulsion Systems Testing Under Simulated Space Conditions at Plum Brook Station's Spacecraft Propulsion Research Facility (B-2)

Preparing NASA's Plum Brook Station's Spacecraft Propulsion Research Facility (B-2) to support NASA's new generation of launch vehicles has raised many challenges for B-2's support staff. The facility provides a unique capability to test chemical propulsion systems/vehicles while simulating space thermal and vacuum environments. Designed and constructed in the early 1960s to support upper stage cryogenic engine/vehicle system development, the Plum Brook Station B-2 facility will require modifications to support the larger, more powerful, and more advanced engine systems for the next generation of vehicles leaving earth's orbit. Engine design improvements over the years have included large area expansion ratio nozzles, greater combustion chamber pressures, and advanced materials. Consequently, it has become necessary to determine what facility changes are required and how the facility can be adapted to support varying customers and their specific test needs. Exhaust system performance, including understanding the present facility capabilities, is the primary focus of this work. A variety of approaches and analytical tools are being employed to gain this understanding. This presentation discusses some of the challenges in applying these tools to this project and expected facility configuration to support the varying customer needs.

Edwards, Daryl A.↗

An Overview of Measurement Comparisons from the INTEX-B/MILAGRO Airborne Field Campaign

As part of the NASA's INTEX-B mission, the NASA DC-8 and NSF C-130 conducted three wing-tip to wing-tip comparison flights. The intercomparison flights sampled a variety of atmospheric conditions (polluted urban, non-polluted, marine boundary layer, clean and polluted free troposphere). These comparisons form a basis to establish data consistency, but also should also be viewed as a continuation of efforts aiming to better understand and reduce measurement differences as identified in earlier field intercomparison exercises. This paper provides a comprehensive overview of 140 intercomparisons of data collected as well as a record of the measurement consistency demonstrated during INTEX-B. It is the primary goal to provide necessary information for the future research to determine if the observations from different INTEX-B platforms/instrument are consistent within the PI reported uncertainties and used in integrated analysis. This paper may also contribute to the formulation strategy for future instrument developments. For interpretation and most effective use of these results, the reader is strongly urged to consult with the instrument principle investigator.

Kleb, Mary M.↗

Space Propulsion Research Facility (B-2): An Innovative, Multi-Purpose Test Facility

The Space Propulsion Research Facility, commonly referred to as B-2, is designed to hot fire rocket engines or upper stage launch vehicles with up to 890,000 N force (200,000 lb force), after environmental conditioning of the test article in simulated thermal vacuum space environment. As NASA s third largest thermal vacuum facility, and the largest designed to store and transfer large quantities of propellant, it is uniquely suited to support developmental testing associated with large lightweight structures and Cryogenic Fluid Management (CFM) systems, as well as non-traditional propulsion test programs such as Electric and In-Space propulsion. B-2 has undergone refurbishment of key subsystems to support the NASA s future test needs, including data acquisition and controls, vacuum, and propellant systems. This paper details the modernization efforts at B-2 to support the Nation s thermal vacuum/propellant test capabilities, the unique design considerations implemented for efficient operations and maintenance, and ultimately to reduce test costs.

Hill, Gerald M.↗

Modernization of B-2 Data, Video, and Control Systems Infrastructure

The National Aeronautics and Space Administration (NASA) Glenn Research Center (GRC) Plum Brook Station (PBS) Spacecraft Propulsion Research Facility, commonly referred to as B-2, is NASA s third largest thermal-vacuum facility with propellant systems capability. B-2 has completed a modernization effort of its facility legacy data, video and control systems infrastructure to accommodate modern integrated testing and Information Technology (IT) Security requirements. Integrated systems tests have been conducted to demonstrate the new data, video and control systems functionality and capability. Discrete analog signal conditioners have been replaced by new programmable, signal processing hardware that is integrated with the data system. This integration supports automated calibration and verification of the analog subsystem. Modern measurement systems analysis (MSA) tools are being developed to help verify system health and measurement integrity. Legacy hard wired digital data systems have been replaced by distributed Fibre Channel (FC) network connected digitizers where high speed sampling rates have increased to 256,000 samples per second. Several analog video cameras have been replaced by digital image and storage systems. Hard-wired analog control systems have been replaced by Programmable Logic Controllers (PLC), fiber optic networks (FON) infrastructure and human machine interface (HMI) operator screens. New modern IT Security procedures and schemes have been employed to control data access and process control flows. Due to the nature of testing possible at B-2, flexibility and configurability of systems has been central to the architecture during modernization.

Cmar, Mark D.↗

Modernization of B-2 Data, Video, and Control Systems Infrastructure

The National Aeronautics and Space Administration (NASA) Glenn Research Center (GRC) Plum Brook Station (PBS) Spacecraft Propulsion Research Facility, commonly referred to as B-2, is NASA's third largest thermal-vacuum facility with propellant systems capability. B-2 has completed a modernization effort of its facility legacy data, video and control systems infrastructure to accommodate modern integrated testing and Information Technology (IT) Security requirements. Integrated systems tests have been conducted to demonstrate the new data, video and control systems functionality and capability. Discrete analog signal conditioners have been replaced by new programmable, signal processing hardware that is integrated with the data system. This integration supports automated calibration and verification of the analog subsystem. Modern measurement systems analysis (MSA) tools are being developed to help verify system health and measurement integrity. Legacy hard wired digital data systems have been replaced by distributed Fibre Channel (FC) network connected digitizers where high speed sampling rates have increased to 256,000 samples per second. Several analog video cameras have been replaced by digital image and storage systems. Hard-wired analog control systems have been replaced by Programmable Logic Controllers (PLC), fiber optic networks (FON) infrastructure and human machine interface (HMI) operator screens. New modern IT Security procedures and schemes have been employed to control data access and process control flows. Due to the nature of testing possible at B-2, flexibility and configurability of systems has been central to the architecture during modernization.

Cmar, Mark D.↗

Solar Simulation for the CREST Preflight Thermal-Vacuum Test at B-2

In June 2011, the multi-university sponsored Cosmic Ray Electron Synchrotron Telescope (CREST) has undergone thermal-vacuum qualification testing at the NASA Glenn Research Center (GRC), Plum Brook Station, Sandusky, Ohio. The testing was performed in the B-2 Space Propulsion Facility vacuum chamber. The CREST was later flown over the Antarctic region as the payload of a stratospheric balloon. Solar simulation was provided by a system of planar infrared lamp arrays specifically designed for CREST. The lamp arrays, in conjunction with a liquid-nitrogen-cooled cold wall, achieved the required thermal conditions for the qualification tests. The following slides accompanied the presentation of the report entitled Solar Simulation for the CREST Preflight Thermal-Vacuum Test at B-2, at the 27th Aerospace Testing Seminar, October 2012. The presentation described the test article, the test facility capability, the solar simulation requirements, the highlights of the engineering approach, and the results achieved. The presentation was intended to generate interest in the report and in the B-2 test facility.

Ziemke, Robert A.↗

Automatic Dependent Surveillance Broadcast (ADS-B) System for Ownership and Traffic Situational Awareness

The present invention proposes an automatic dependent surveillance broadcast (ADS-B) architecture and process, in which priority aircraft and ADS-B IN traffic information are included in the transmission of data through the telemetry communications to a remote ground control station. The present invention further proposes methods for displaying general aviation traffic information in three and/or four dimension trajectories using an industry standard Earth browser for increased situation awareness and enhanced visual acquisition of traffic for conflict detection. The present invention enable the applications of enhanced visual acquisition of traffic, traffic alerts, and en-route and terminal surveillance used to augment pilot situational awareness through ADS-B IN display and information in three or four dimensions for self-separation awareness.

Arteaga, Ricardo A.↗

NASA GSFC Report on CCSDS Recommendations 2.1.8A B Minimum Earth Station Transmitter Frequency Resolution for Spacecraft Receiver Acquisition

In Fall 2016, ESA presented paper SLS-RFM 16-10 documenting a possible issue with the frequency lock-in range specification in Recommendation 2.1.8A of typically 267 to 1067 Hz in considerings (b) from considerings (a) for loop bandwidths [2B(sub LO)] in the range of 200 to 800 Hz with a recommendation of 100 Hz step size for frequency sweeping. The paper calculated the lock-in range to be (+/-)266 to (+/-)1064 rad/s or (+/-)42 to (+/-)168 Hz. Also, Recommendation 2.1.8B has the same issue for considering (a) and (b), i.e. for 2B(sub LO) =10 Hz, a lock-in range of 13 Hz was specified and a recommendation of 5 Hz step size for frequency sweeping. ESA also provided test results from the Rosetta and Exomars transponders. The results were somewhat inconsistent since the tests to verify lock-in and pull-in range did not include acquisition time, which is vital to the definition of these performance measures. This paper will address these test results below. However, we first examine the rationale for Recommendation 2.1.8A/B and its consistency with the theory of 2nd order phase lock loop operations. Our approach is to design a digital phase locked loop (DPLL) from phase locked loop (PLL) requirements. All analysis will be performed with a DPLL.

digital phase locked loop↗

The Spectral Invariant Approximation Within Canopy Radiative Transfer to Support the Use of the EPIC/DSCOVR Oxygen B-band for Monitoring Vegetation

EPIC (Earth Polychromatic Imaging Camera) is a 10-channel spectroradiometer onboard DSCOVR (Deep Space Climate Observatory) spacecraft. In addition to the near-infrared (NIR, 780 nm) and the 'red' (680 nm) channels, EPIC also has the O2 A-band (764+/-0.2 nm) and B-band (687.75+/-0.2 nm). The EPIC Normalized Difference Vegetation Index (NDVI) is defined as the difference between NIR and 'red' channels normalized to their sum. However, the use of the O2 B-band instead of the 'red' channel mitigates the effect of atmosphere on remote sensing of surface reflectance because O2 reduces contribution from the radiation scattered by the atmosphere. Applying the radiative transfer theory and the spectral invariant approximation to EPIC observations, the paper provides supportive arguments for using the O2 band instead of the red channel for monitoring vegetation dynamics. Our results suggest that the use of the O2 B-band enhances the sensitivity of the top-of-atmosphere NDVI to the presence of vegetation.

Alexander Marshak↗

NASA Update to WG-B: MMS GPS Performance at 29.34 Re (50% of Way to the Moon)

NASA represents US civil space users at the United Nations International Committee on Global Navigation Satellite Systems (ICG). ICG Working Group B (WG-B) is responsible for Enhancement of GNSS Performance, New Services and Capabilities. The development and characterization of the GNSS Space Service Volume (SSV) is a key area of activity for NASA within WG-B. This presentation describes the MMS performance employing GPS at 50% of the way to the moon.

Global Navigation Satellite Systems↗

Compositional Constraints on the Best Characterized Rocky Exoplanet, Kepler-36 b

Kepler-36 is an extreme planetary system, consisting of two transiting sub-Neptune-size planets orbiting around a sub-giant star with periods of 13.84 and 16.24 days. Mutual gravitational interactions between the two planets perturb the planets' transit times, allowing the planets' masses to be measured. Despite the similarity of their masses and orbital radii, the planets show a stark contrast in their mean densities: the inner planet (Kepler-36 b) is more than eight times as dense as its outer companion planet (Kepler-36 c). We perform a photo-dynamical analysis of the Kepler-36 system based on more than three years of Kepler photometry. With N-body integrations of initial conditions sampled from the photo-dynamical fits, we further refine the properties of the system by ruling out solutions that show large scale instability within 5 billion days. Ultimately, we measure the planets' masses with 4.2% precision and the planets' radii with 1.8% precision. Kepler-36 b is the rocky exoplanet with the most precisely measured mass and radius. Kepler-36 b's mass and radius are consistent with an Earth-like composition, whereas an iron-enhanced Mercury-like composition is ruled out.

sub-Neptune-size planets↗

Transit Timing Variations for AU Microscopii b and c

We explore the transit timing variations (TTVs) of the young (22 Myr) nearby AU Mic planetary system. For AU Mic b, we introduce three Spitzer (4.5 μm) transits, five TESS transits, 11 LCO transits, one PEST transit, one Brierfield transit, and two transit timing measurements from Rossiter–McLaughlin observations; for AU Mic c, we introduce three TESS transits. We present two independent TTV analyses. First, we use EXOFASTv2 to jointly model the Spitzer and ground-based transits and obtain the midpoint transit times. We then construct an O − C diagram and model the TTVs with Exo-Striker. Second, we reproduce our results with an independent photodynamical analysis. We recover a TTV mass for AU Mic c of -10.8+2.22.3 M⊕. We compare the TTV-derived constraints to a recent radial velocity (RV) mass determination. We also observe excess TTVs that do not appear to be consistent with the dynamical interactions of b and c alone or due to spots or flares. Thus, we present a hypothetical nontransiting “middle-d” candidate exoplanet that is consistent with the observed TTVs and candidate RV signal and would establish the AU Mic system as a compact resonant multiplanet chain in a 4:6:9 period commensurability. These results demonstrate that the AU Mic planetary system is dynamically interacting, producing detectable TTVs, and the implied orbital dynamics may inform the formation mechanisms for this young system. We recommend future RV and TTV observations of AU Mic b and c to further constrain the masses and confirm the existence of possible additional planet(s).

Justin M Wittrock↗