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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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DOE OSTI2025

PCM1 coordinates centrosome asymmetry with polarized endosome dynamics to regulate daughter cell fate

Vertebrate radial glia progenitors (RGPs) balance self-renewal and differentiation through asymmetric cell division (ACD), which involves unequal centrosome inheritance. How centrosome asymmetry directs cell fate remains poorly understood. Here, we identify Pericentriolar material 1 (Pcm1) as a key player in this process. In zebrafish embryonic RGPs, Pcm1 is asymmetrically associated with Cep83, a mother centrosome marker. Using in vivo time-lapse imaging and nanoscale-resolution expansion microscopy, we detect Pcm1 on Notch ligand-containing endosomes, where it interacts–either directly or indirectly–with Par-3 and dynein. Loss of pcm1 disrupts endosome dynamics, increasing neuronal differentiation at the expense of RGP self-renewal. Mechanistically, Pcm1 facilitates the transition from Rab5b to Rab11a and promotes the assembly of Par-3 and dynein macromolecular complexes on recycling endosomes. Furthermore, we find conserved PARD3-PCM1-CEP83-RAB11 associations in human cortical brain organoids. Our findings uncover that Pcm1 links centrosome asymmetry to polarized endosome trafficking, thereby regulating RGP fate decisions.

Cell fate and cell lineage↗

DOE OSTI2025

Exploring single-cell biosynthetic noise and dynamics for enhanced betaxanthin production in Escherichia coli

Cell-to-cell variability often limits the efficiency of microbial bioproduction, yet how individual cells fluctuate over time and how these fluctuations shape population-level output remain unclear. To address this issue, we tracked a heterologous betaxanthin pathway in Escherichia coli using microfluidics-assisted time-lapse microscopy, allowing simultaneous measurement of fluctuations in betaxanthin, its biosynthetic enzyme DOD and growth across generations. Here we show that over 50% of high betaxanthin producers become medium or low producers after two divisions. Betaxanthin variation primarily originates from DOD noise, with a smaller contribution from growth rate fluctuations. We further develop a stochastic model to explore various control circuits and find that pathway enzyme or metabolite-based growth selection strategies are most effective in enhancing production. We experimentally validate the model by coupling enzyme expression to nutrient availability, which enriches high producers and boosts titer by 4.4-fold. Our results highlight key sources of metabolic heterogeneity and provide a framework for designing robust microbial processes.

Bacterial systems biology↗

DOE OSTI2025

Dataset for "A Microfluidic Spore Chamber for Long-Term Imaging of Single-Spore Hyphal Development"

Understanding the life cycle of fungal spores is essential for elucidating their roles in pathogenesis, dispersal, and survival. However, studying spore development under controlled, spatially defined conditions remains challenging. Here, we present the Spore Chamber, a custom-built microfluidic platform engineered for parallel trapping and long-term imaging of individual spores under defined media conditions, enabling real-time visualization of hyphal development. Using Aspergillus fumigatus as a model organism, we demonstrate that sparse trapping of individual spores within size-matched trap geometries enables long-term time-lapse imaging of key developmental stages, including germination, polarized hyphal elongation, branching, and conidiophore formation. To assess the device’s capacity to resolve morphogenetic responses to exogenous signals, we introduced lipochitooligosaccharides (LCOs) and short-chain chitooligosaccharides (COs). Rhizobium-derived, non-sulfated LCO (nsLCO) mixtures induced enhanced secondary branching (hyperbranching), a response not previously reported in A. fumigatus under these signal conditions, to our knowledge, whereas sulfated LCOs and CO4 did not significantly alter branching patterns. In addition, long-term confinement and imaging revealed rare developmental morphologies previously described primarily in mutant strains, including split conidiophore formation, elongated phialides, microcyclic conidiation, and chlamydospore development. Together, these results establish the Spore Chamber as a targeted microfluidic platform for single-spore phenotyping and long-term developmental analysis, with applications in fungal biology, chemical signaling studies, and host–microbe interaction research. Videos of the observed phenomena are included in this data set.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2024

Monitoring the impact of confinement on hyphal penetration and fungal behavior

Through their expansive mycelium network, soil fungi alter the physical arrangement and chemical composition of their local environment. This can significantly impact bacterial distribution and nutrient transport and can play a dramatic role in shaping the rhizosphere around a developing plant. However, direct observation and quantitation of such behaviors is extremely difficult due to the opacity and complex porosity of the soil microenvironment. In this study, we demonstrate the development and use of an engineered microhabitat to visualize fungal growth in response to varied levels of confinement. Microfluidics were fabricated using photolithography and conventional soft lithography, assembled onto glass slides, and prepared to accommodate fungal cultures. Selected fungal strains across three phyla (Ascomycota: Morchella sextalata , Fusarium falciforme ; Mucoromycota: Linnemannia elongata , Podila minutissima , Benniella ; Basidiomycota: Laccaria bicolor , and Serendipita sp.) were cultured within microhabitats and imaged using time-lapse microscopy to visualize development at the mycelial level. Fungal hyphae of each strain were imaged as they penetrated through microchannels with well-defined pore dimensions. The hyphal penetration rates through the microchannels were quantified via image analysis. Other behaviors, including differences in the degree of branching, peer movement, and tip strength were also recorded for each strain. Our results provide a repeatable and easy-to-use approach for culturing fungi within a microfluidics platform and for visualizing the impact of confinement on hyphal growth and other fungal behaviors pertinent to their remodeling of the underground environment.

Guo, Yi-Syuan (ORCID:0000000340142225)↗

DOE OSTI2025

Electrical Resistivity Tomography data from 2016 to 2018 at the Lower Montane site in the East River Watershed, Colorado

This dataset contains time-lapse Electrical Resistivity Tomography (ERT) data along a transect located on the northeast-facing hillslope at the lower montane site (Pumphouse site) in the upper East River Watershed. The monitoring dataset covers the period from November 2, 2016, to August 6, 2018. In addition, the archive also contains a baseline dataset from October 9, 2016. The ERT transect consisted of 128 electrodes with an electrode spacing of 1.25 m. The acquisition system was located in the middle of the transect, about 50 m on one side, and included an MPT (Multi-Phase Technologies) ERT system, a mini computer, and batteries with solar panels. Acquisition occurred daily under normal circumstances. The first 16 electrodes (from the upper end of the transect) could not be used after the cable was damaged during the 2017–2018 winter. Also, due to multiple failures in the power system, the temporal resolution of the data is much lower in 2018 compared to 2016 and 2017. The data have been processed and used in Dafflon et al., 2023, and the baseline dataset was used in Falco et al., 2019 (see reference list). This archive contains the measurements (ER.zip containing csv files) for each of the 326 acquisition times and a filtered version where only electrodes 17 to 128 are included (ERT_sm.zip containing csv files). The archive also contains the baseline dataset and two acquisitions with full reciprocals (ERT_RB.zip containing csv files), as well as all the raw MPT files (ERT_raw_MTP.zip). The geometry (electrode position and elevation) is provided in Universal Transverse Mercator (UTM) 13N Geoid2012AB in the file named ERT_Location.csv. The archive contains 1 *.csv data files, four *.zip files, and three metadata *.csv files. This work was supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2025

AmeriFlux FLUXNET-1F US-Prr Poker Flat Research Range Black Spruce Forest

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-Prr Poker Flat Research Range Black Spruce Forest. This is the FLUXNET version of the carbon flux data for the site US-Prr Poker Flat Research Range Black Spruce Forest produced by applying the standard ONEFlux (1F) software. Site Description - This site is located in a blackspruce forest within the property of the Poker Flat Research Range, University of Alaska, Fairbanks. Time-lapse image of the canopy is measured at the same time to relate flux data to satellite images.

Iwahana, Go [University of Alaska, Faribanks]↗

DOE OSTI2025

Integration and Demonstration of Monitoring, Modeling, and Prediction of DV-1 Amendment Performance at the Bench Scale: DV-1 Amendment Demonstration

During fiscal years 2024 and 2025, the U.S. Department of Energy’s Hanford Field Office commissioned Pacific Northwest National Laboratory to conduct applied research aimed at reducing the cost, time, and uncertainty associated with in situ treatment of vadose zone contaminants at the Hanford Site. This report outlines the integration of three key research efforts into a meso-scale demonstration designed to advance field-scale solutions that aim to (1) optimize the delivery of chemical amendments to contaminated soils, (2) reduce uncertainty in amendment delivery performance assessment using advanced monitoring techniques, and (3) provide real-time insights into when and where amendment-induced precipitation reactions occur in the subsurface. To achieve these objectives, the tank-scale (~ 1 cubic meter) Geophysical Imaging of Flow and Transport (GIFT) system was developed. GIFT enables experimental testing of amendment delivery while incorporating automated multi-modal monitoring approaches, including pressure measurements, direct fluid sampling, and remote time-lapse geophysical imaging. The data generated from these monitoring techniques will serve as inputs for a generative artificial-intelligence-driven digital twin – a numerical simulation model designed to honor observed data while quantifying uncertainty in simulation accuracy. Using this simulator, researchers will refine an amendment injection strategy to maximize delivery efficiency within a low-permeability soil zone. Monitoring data will be interpreted through simulated outputs to enhance understanding of the injection process. The efficacy of this integrated approach will be evaluated through direct sampling at the conclusion of the experiment.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

DOE OSTI2025

Phase II Field Demonstration at Lansing Smith Generating Plant, Southport, Florida (Final Report)

The Final Technical Report: Field Test Design and Pressure Management Strategies for Phase II Field Demonstration of Optimal Pressure Control, Plume Management, and Produced Water Strategies presents the culmination of multi-year efforts under the U.S. Department of Energy’s Brine Extraction and Storage Test (BEST) program, led by EPRI in partnership with Gulf Power at the Plant Smith site near Panama City, Florida. The project was designed to evaluate and demonstrate the technical feasibility of managing subsurface pressures and fluid movement associated with large-scale CO₂ injection, using low-salinity water as a proxy fluid. Through a combination of field injection testing, reservoir modeling, and optimization studies, the research team developed and refined active and passive brine extraction strategies aimed at controlling injection-induced pressure buildup, mitigating risks of fault activation, and managing plume migration. The field demonstration incorporated a new injection well (TIW-2), a new monitoring/extraction well (TEMW-A), and an existing well (TIW-1) repurposed for passive pressure relief. Complementary geophysical monitoring was designed to track plume development and evaluate the effectiveness of pressure management strategies. The report details the integrated workflow encompassing wellfield development, regulatory permitting, model calibration, and survey design. It includes a comprehensive electromagnetic (EM) modeling and inversion study used to develop a cost-effective, time-lapse geophysical monitoring plan capable of imaging the evolving low-salinity plume within the highly saline Lower Tuscaloosa formation. Reservoir simulation results guided the design of the 17-month injection program and the timing of active extraction to maintain formation pressures below a hypothetical fault reactivation threshold. Supporting analyses evaluated potential injectivity risks related to clay fines migration and geochemical incompatibility, identifying practical mitigation measures such as salinity and pH control. The final design recommends a series of crosswell EM surveys—conducted before, during, and after injection—as the most effective and economical approach for plume imaging, supplemented by continuous downhole pressure and flow monitoring. Collectively, the study provides a field-ready framework for cost-effective pressure management and monitoring in support of future CO₂ storage projects.

01 COAL, LIGNITE, AND PEAT↗

DOE OSTI2025

Where do the fish go in winter? A year of observing seasonal changes in Sequim Bay’s nearshore fish community

The use of environmental DNA (eDNA) sampling has been proposed as a complementary method to monitor fish species in marine environments. eDNA offers a non-invasive, cost-effective, and scalable way to detect aquatic species. It is appealing in environments where traditional methods are limited by access or visibility, especially in complex or sensitive habitats such as tidal channels and other marine energy sites. Before eDNA can be fully relied upon, we must verify its accuracy against established methods, like underwater photography. In this study, we collected eDNA samples and concurrently deployed a 360-degree camera near the floating dock of PNNL-Sequim in the tidal channel of Sequim Bay once a month for twelve consecutive months. During deployments, the camera remained on the seafloor for several hours overlapping slack tide and captured time-lapse photographs at ten second intervals. Counts and identifications of fish species observed in the images were used to calculate monthly Shannon diversity and Pielou evenness indices. These values were compared across the months using a Kruskal-Wallis test paired with a Conover-Iman post-hoc test. Cliff’s Delta was also calculated to quantify the effect size of the monthly differences. No fish were observed from December through April, likely due to seasonal behavior changes within the local fish community. Fish returned to the shoreline in May, with the greatest diversity and evenness recorded in September. These findings reveal substantial seasonal variation in nearshore fish communities. Many monthly comparisons were found to contain statistically significant differences within the diversity and evenness, and even more were found to have large effect sizes, signifying large ecological changes throughout the seasons. The absence of fish observed during the winter months is a key outcome of this survey that will hopefully be reflected in the eDNA results still to come, which would help validate the eDNA approach.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS1965

Currents and photogrammetry

Stereo time-lapse photography to measure speed of moving objects by false parallax produced by motion between photos - photogrammetry

PHOTOGRAMMETRY↗

NASA NTRS1971

Intensity oscillation in H alpha-fine structures

Using a new technique of directly measuring the intensity variation from the 16mm time-lapse filtergram movies taken in the blue wing and in the line center of H-alpha, periodic intensity oscillations were found in the center of H-alpha supergranulation network, in rosette centers, and in plage granules. The oscillatory period of intensity in the network is of the order of 170 + or - 44 seconds while in regions of stronger magnetic field, such as in plages and in rosettes, the period was found to be longer, on the order of 300 + or - 50 seconds. It is suggested that observed intensity oscillation in the rosette center is related to the shooting out of dark mottles from rosettes. Oscillatory intensity fluctuations were also observed in the sunspot umbra.

Bhatnagar, A.↗

NASA NTRS1971

The pointing errors of geosynchronous satellites

A study of the correlation between cloud motion and wind field was initiated. Cloud heights and displacements were being obtained from a ceilometer and movie pictures, while winds were measured from pilot balloon observations on a near-simultaneous basis. Cloud motion vectors were obtained from time-lapse cloud pictures, using the WINDCO program, for 27, 28 July, 1969, in the Atlantic. The relationship between observed features of cloud clusters and the ambient wind field derived from cloud trajectories on a wide range of space and time scales is discussed.

Sikdar, D. N.↗

NASA NTRS1972

Intensity oscillation in H-alpha fine structure.

Using a new technique of directly measuring the intensity variation from the 16 mm time-lapse filtergram movies taken in the blue wing and in the line center of H-alpha, we found periodic intensity oscillations in the center of H-alpha supergranulation network, in rosette centers and in plage granules. The oscillatory period of intensity in the network is of the order of 170 plus or minus 44 seconds while in regions of stronger magnetic field, such as in plages and in rosettes, the period was found to be longer, on the order of 300 plus or minus 50 seconds. It is suggested that observed intensity oscillation in the rosette center is related to the shooting out of dark mottles from rosettes. Oscillatory intensity fluctuations have been also observed in the sunspot umbra.

Bhatnagar, A.↗

NASA NTRS1973

Plume development in Long Island Sound observed by remote sensing (ERTS-1)

As the Connecticut River flows into Long Island Sound, large plumes are developed during the mixing of ocean and estuarine waters. Plumes were delineated for July 28, October 8, October 27, and December 2, 1972, by analyzing ERTS-1 imagery with the SRI electronic satellite image analysis console (ESIAC). Insertion of MSS band 5 into the ESIAC produced the best result in this analysis. The four plumes that have been delineated provide the first input to a time-lapse analysis of circulation patterns at the eastern end of Long Island Sound.

Ruggles, F. H., Jr.↗

NASA NTRS1973

Dynamics of suspended sediment plumes in Lake Ontario

The author has identified the following significant results. Enhancement of ERTS-1 imagery yielded excellent quality 35-mm color slides and prints of several prominent turbidity plumes in Lake Ontario. Selected ERTS-1 frames of the Welland Canal and Genesee River plumes will be used to develop time-lapse sequences showing the impact of wind stress on each plume. Unusually high lake levels during the spring resulted in extensive beach erosion along the entire Lake Ontario shoreline. The resulting high concentrations of suspended matter generated highly turbid (up to 420 JTU) nearshore conditions that appeared milky white in the imagery obtained April 12 and 29th, 1973. During the shipping season, both the Welland Canal and a diversion channel at Port Dalhousie, Ontario, produced readily identifiable turbidity plumes in Lake Ontario. However, in the winter neither plume was visible in the ERTS-1 imagery suggesting sharply lower sediment discharge into Lake Ontario from these sources.

Pluhowski, E. J.↗

NASA NTRS1973

Exhaust cloud rise and growth for Apollo Saturn engines.

Empirical and analytical results have been reduced from time-lapse photographs of exhaust clouds during the launch of Apollo 10, 14, 15, and 16 from the Kennedy Space Center. Theoretical predictions of the buoyant cloud rise and growth compare very favorably to actual measurements. The mathematical model predicts with engineering accuracy the terminal altitude and size of the clouds. A principal input to MSFC's multilayer diffusion model is top height of the exhaust cloud. It is this characteristic dimension that is the main subject of this report. An interesting occurrence during launch is the formation of bimodal exhaust cloud which is due to the north-south oriented flame trench directly beneath the base of the vehicle. This study has added considerable information on engine exhaust cloud rise and growth which is essential to the study of atmospheric dispersal in time and space.

Susko, M.↗

NASA NTRS1974

Image reproduction with interactive graphics

Software application or development in optical image digital data processing requires a fast, good quality, yet inexpensive hard copy of processed images. To achieve this, a Cambo camera with an f 2.8/150-mm Xenotar lens in a Copal shutter having a Graflok back for 4 x 5 Polaroid type 57 pack-film has been interfaced to an existing Adage, AGT-30/Electro-Mechanical Research, EMR 6050 graphic computer system. Time-lapse photography in conjunction with a log to linear voltage transformation has resulted in an interactive system capable of producing a hard copy in 54 sec. The interactive aspect of the system lies in a Tektronix 4002 graphic computer terminal and its associated hard copy unit.

Buckner, J. D.↗

NASA NTRS1974

Multi-slit spectrograph and H alpha Doppler system

Description of the design and operation of a multiple-entrance-slit spectrograph, built for time-lapse photography of the spectra of flares and other transient solar phenomena. This spectrograph employs narrow-band filters to limit the wavelength range of the spectrograph to a few angstroms centered at H-alpha or other spectral lines. A polarizing beamsplitter, prior to the slits, provides a prefiltered second solar image to a narrow band H-alpha Doppler filter for simultaneous photography in the wings of the H-alpha line. The spectrograph and the H-alpha Doppler system constitute a flexible instrument in which components may be substituted to achieve different passband widths, image scales, dispersions, and corresponding numbers and spacings of adjacent spectra at a selected wavelength.

Martin, S. F.↗