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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 37 records · Page 2

Radiometric Stability in 16 years of AIRS Hyperspectral Infrared Data (SPIE OP-431-20)

With global warming at the rate of 10 mK/yr, it is important to at least characterize any trend in the AIRS data, which may impact the use of AIRS data for climate change research. We evaluated the stability of the AIRS v5 calibration for seven atmospheric window channels between 2002 and 2018 under tropical ocean clear conditions. Trends for the channels between 961, 1128 and 1231 cm-1 channels are typically +3 mK/yr; for 790 and 901 cm-1 the trend is 6 mK/yr, i.e. the observations are increasingly getting warmer than expected. The trends are day/night consistent. The trend for the 2508 and 2616 cm-1 channels is close to 10 mK/yr at night, but closer to 6 mK/yr during the day. On an absolute scale these trends are small, but not when viewed in the context of global warming at a 10 mK/yr rate. While the warming trends are consistent with increased scattering from the scan mirrors, which create an error in reading the Onboard Blackbody Calibration, the resulting changes in the gain are a factor of about five larger than observed changes in the gain. The effects of scattering due to scan mirror contamination are evident at extremely cold temperatures, but scattering does not produce the observed warming at warm temperatures. It is possible that much of the observed warming in the AIRS window channels is a geophysical effect related to the warming of the oceans, resulting in a shift in the diurnal cycle and skin effect correction. This requires more careful evaluation.

Manning, Evan M.↗

Fault-Tolerant, Multiple-Zone Temperature Control

A computer program has been written as an essential part of an electronic temperature control system for a spaceborne instrument that contains several zones. The system was developed because the temperature and the rate of change of temperature in each zone are required to be maintained to within limits that amount to degrees of precision thought to be unattainable by use of simple bimetallic thermostats. The software collects temperature readings from six platinum resistance thermometers, calculates temperature errors from the readings, and implements a proportional + integral + derivative (PID) control algorithm that adjusts heater power levels. The software accepts, via a serial port, commands to change its operational parameters. The software attempts to detect and mitigate a host of potential faults. It is robust to many kinds of faults in that it can maintain PID control in the presence of those faults.

Granger, James↗

VirION2: a short- and long-read sequencing and informatics workflow to study the genomic diversity of viruses in nature

Microbes play fundamental roles in shaping natural ecosystem properties and functions, but do so under constraints imposed by their viral predators. However, studying viruses in nature can be challenging due to low biomass and the lack of universal gene markers. Though metagenomic short-read sequencing has greatly improved our virus ecology toolkit—and revealed many critical ecosystem roles for viruses—microdiverse populations and fine-scale genomic traits are missed. Some of these microdiverse populations are abundant and the missed regions may be of interest for identifying selection pressures that underpin evolutionary constraints associated with hosts and environments. Though long-read sequencing promises complete virus genomes on single reads, it currently suffers from high DNA requirements and sequencing errors that limit accurate gene prediction. Here we introduce VirION2, an integrated short- and long-read metagenomic wet-lab and informatics pipeline that updates our previous method (VirION) to further enhance the utility of long-read viral metagenomics. Using a viral mock community, we first optimized laboratory protocols (polymerase choice, DNA shearing size, PCR cycling) to enable 76% longer reads (now median length of 6,965 bp) from 100-fold less input DNA (now 1 nanogram). Using a virome from a natural seawater sample, we compared viromes generated with VirION2 against other library preparation options (unamplified, original VirION, and short-read), and optimized downstream informatics for improved long-read error correction and assembly. VirION2 assemblies combined with short-read based data (‘enhanced’ viromes), provided significant improvements over VirION libraries in the recovery of longer and more complete viral genomes, and our optimized error-correction strategy using long- and short-read data achieved 99.97% accuracy. In the seawater virome, VirION2 assemblies captured 5,161 viral populations (including all of the virus populations observed in the other assemblies), 30% of which were uniquely assembled through inclusion of long-reads, and 22% of the top 10% most abundant virus populations derived from assembly of long-reads. Viral populations unique to VirION2 assemblies had significantly higher microdiversity means, which may explain why short-read virome approaches failed to capture them. These findings suggest the VirION2 sample prep and workflow can help researchers better investigate the virosphere, even from challenging low-biomass samples. Our new protocols are available to the research community on protocols.io as a ‘living document’ to facilitate dissemination of updates to keep pace with the rapid evolution of long-read sequencing technology.

Long-reads↗

Deferred electronic heterodyne moire deflectometry: A method for transient density fields measurement

Effects of spherical aberrations of the mirror used in the moire system on the angular resolution of the system are investigated. It is shown that the spherical aberrations may reduce significantly the performance of the conventional moire deflectometer. However, due to the heterodyne procedure, this is not the case with the heterodyne moire system. A moire system with a constant speed moving grating is demonstrated. It is shown that the system readout is linear and the system does not need calibration. In addition, the repeatability of the measurements is improved in this system as compared to the sinusoidally moving grating setup. The problem of the photographic plates alignment is solved by using a mechanical system in which the plate is held firmly throughout the experiment and accurately replaced after removing for photographic processing. The effect of a circular detector's aperture size on readout was tested. It is shown that the spatial phase variations, observed when scanning along a straight moire fringe, may considerably be reduced. At present we may say that both the on-line and the deferred heterodyne moire techniques may reliably be used. The errors of phase readings are 1 deg and 5 deg for the on-line and deferred methods. The total error due to subtraction of two readings at each position is, therefore, 1.4 deg and 7 deg, respectively. Further research for improving the deferred system is suggested.

Stricker, Josef↗

polishCLR: A Nextflow Workflow for Polishing PacBio CLR Genome Assemblies

Long-read sequencing has revolutionized genome assembly, yielding highly contiguous, chromosome-level contigs. However, assemblies from some third generation long read technologies, such as Pacific Biosciences (PacBio) continuous long reads (CLR), have a high error rate. Such errors can be corrected with short reads through a process called polishing. Although best practices for polishing non-model de novo genome assemblies were recently described by the Vertebrate Genome Project (VGP) Assembly community, there is a need for a publicly available, reproducible workflow that can be easily implemented and run on a conventional high performance computing environment. Here, we describe polishCLR (https://github.com/isugifNF/polishCLR), a reproducible Nextflow workflow that implements best practices for polishing assemblies made from CLR data. PolishCLR can be initiated from several input options that extend best practices to suboptimal cases. It also provides re-entry points throughout several key processes, including identifying duplicate haplotypes in purge_dups, allowing a break for scaffolding if data are available, and throughout multiple rounds of polishing and evaluation with Arrow and FreeBayes. PolishCLR is containerized and publicly available for the greater assembly community as a tool to complete assemblies from existing, error-prone long-read data.

59 BASIC BIOLOGICAL SCIENCES↗

Carbon monoxide measurement in the global atmospheric sampling program

The carbon monoxide measurement system used in the NASA Global Atmospheric Sampling Program (GASP) is described. The system used a modified version of a commercially available infrared absorption analyzer. The modifications increased the sensitivity of the analyzer to 1 ppmv full scale, with a limit of detectability of 0.02 ppmv. Packaging was modified for automatic, unattended operation in an aircraft environment. The GASP system is described along with analyzer operation, calibration procedures, and measurement errors. Uncertainty of the CO measurement over a 2-year period ranged from + or - 3 to + or - 13 percent of reading, plus an error due to random fluctuation of the output signal + or - 3 to + or - 15 ppbv.

Dudzinski, T. J.↗

Determining diagnostic coverage for memory using redundant execution

Memory, used by a computer to store data, is generally prone to faults, including permanent faults (i.e. relating to a lifetime of the memory hardware), and also transient faults (i.e. relating to some external cause) which are otherwise known as soft errors. Since soft errors can change the state of the data in the memory and thus cause errors in applications reading and processing the data, there is a desire to characterize the degree of vulnerability of the memory to soft errors. In particular, once the vulnerability for a particular memory to soft errors has been characterized, cost/reliability trade-offs can be determined, or soft error detection mechanisms (e.g. parity) may be selectively employed for the memory. In some cases, memory faults can be diagnosed by redundant execution and a diagnostic coverage may be determined.

Bramley, Richard Gavin↗

Liveness as a factor to evaluate memory vulnerability to soft errors

Memory, used by a computer to store data, is generally prone to faults, including permanent faults (i.e. relating to a lifetime of the memory hardware), and also transient faults (i.e. relating to some external cause) which are otherwise known as soft errors. Since soft errors can change the state of the data in the memory and thus cause errors in applications reading and processing the data, there is a desire to characterize the degree of vulnerability of the memory to soft errors. In particular, once the vulnerability for a particular memory to soft errors has been characterized, cost/reliability trade-offs can be determined, or soft error detection mechanisms (e.g. parity) may be selectively employed for the memory. In some cases, memory faults can be diagnosed by redundant execution and a diagnostic coverage may be determined.

Bramley, Richard Gavin↗

The Effect of Large Angles of Yaw on the Accuracy of Wing-Tip Yawmeters

The present method used by the NACA for the measurement of sideslip angles in flight involves the use of a device called the yawmeter. The operation of this instrument depends on the motion of a free-swinging vane which, mounted ahead of the wing tip, alines itself with the local wind direction. Because of the flow pattern about the airplane, the local wind direction at the yaw vane may be slightly different from the direction of the relative wind and the yaw-vane readings may be in error. This error is corrected by using half the difference between the readings of two vanes, one on each wing, for unyawed flight as a calibration constant. It is possible, however, that, because of the change in location of the vane with respect to the flow pattern at large angles of yaw, the constant obtained for unyawed flight may not apply. The present report covers power-off tests made in the free-flight tunnel to check the validity of this method.

Golden, Jacob↗

NovaDemux v39.07

This program is a sequence demultiplexer intended primarily for, but not limited to, Illumina sequencing machines. Typically, multiple experiments ("libraries") are pooled together and sequenced at once, with genetic molecules of these libraries tagged with a synthetic DNA "barcode". After sequencing, the data is demultiplexed into one file per library based on the barcode. However, errors in barcode reading cause misassignment and decrease yield. NovaDemux uses advanced statistical methods to maximize yield while minimizing misassignment compared to existing software.

Bushnell, Brian [Lawrence Berkeley National Labora↗

A Conceptual Design for a Mobile Application to Support Infield Inventory Activities

This paper introduces an inventory assistant being developed at Oak Ridge National Laboratory (ORNL) that we believe will empower users to perform inventory activities at nuclear facilities more accurately, reliably, and quickly. Inventory activities at nuclear facilities are often conducted using pen and paper, which can be time-consuming, tedious, and susceptible to reading or transcription errors. The proposed inventory assistant would replace the paper-based process used by International Atomic Energy Agency (IAEA) inspectors, nuclear facility operators, or verification monitors to complete an inventory of nuclear and non-nuclear items. In general, the inventory assistant would ingest an inventory list, distribute assigned items from the inventory list to one or more mobile devices, enable inventory teams to record their observations in the field, and then enable an inventory lead to integrate and reconcile the observations to produce a final report. The assistant consists of two software components—one for the inventory teams to record observations in the field (In-Field Observations App [IFOA]) and one for the inventory lead to reconcile the inventory list with observations (Distribution, Integration, and Reconciliation Application [DIRA]). This paper introduces the overall workflow of the inventory assistant and describes the IFOA user experience in more detail. To demonstrate the concept, the authors present a use case of IAEA inspectors conducting item counting and tag checking activities of UF6 cylinders at a gas centrifuge enrichment plant with a large number of UF6 cylinders (e.g., thousands). These activities can currently require 30–40 person-days of inspection to complete. Based on experiences during an exercised performed at the IAEA by the ORNL team in 2016, we believe an inventory assistant could allow the IAEA to complete item counting and tag checking using the global identifier or the operator’s barcode in 8–10 person-days of inspection. We would expect other users (e.g., facility operators or verification monitors) to also benefit from significant time savings.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

A Conceptual Design for a Desktop Application to Support Inventory Reconciliation Activities

This paper describes desktop software being developed at Oak Ridge National Laboratory (ORNL) to empower users to reconcile observations about items at nuclear facilities more accurately, reliably, and quickly. Inventory activities at nuclear facilities are often conducted using pen and paper, which can be time-consuming, tedious, and susceptible to reading or transcription errors. The proposed inventory assistant would be a replacement for the paper-based process that could be used by International Atomic Energy Agency (IAEA) inspectors, nuclear facility operators, or treaty verification monitors to conduct an inventory of nuclear and non-nuclear items in a more timely and accurate manner. Garner, McGirl, and Whitaker previously reported their work about a conceptual design for a mobile app to assist users in the field. In general, the inventory assistant would ingest an inventory list, distribute assigned items from the inventory list to one or more mobile devices, enable inventory teams to record their observations in the field, and then enable an inventory lead to integrate and reconcile the observations to produce a final report. The assistant consists of two software components - one for the inventory teams to record observations in the field (In-Field Observations App [IFOA]) and one for the inventory lead to (1) distribute the inventory list to each team, (2) integrate the observations from each team, and (3) reconcile the inventory list with observations (Distribution, Integration, and Reconciliation Application [DIRA]). This paper reviews the inventory assistant workflow and describes the DIRA user experience in more detail. As an example, the authors have chosen to follow the use case of IAEA inspectors conducting item counting and tag checking activities of UF 6 cylinders at a gas centrifuge enrichment plant with a large number of UF 6 cylinders (e.g., thousands). These activities can currently require 30 - 40 person-days of inspection to complete. The authors believe an inventory assistant could allow the IAEA to complete item counting and tag checking using the global identifier or the operator’s barcode in 8 - 10 person-days of inspection. We would expect other users (e.g., facility operators or treaty verification monitors) to also benefit from significant time savings.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

NASA Aviation Safety Reporting System

Problems in briefing of relief by air traffic controllers are discussed, including problems that arise when duty positions are changed by controllers. Altimeter reading and setting errors as factors in aviation safety are discussed, including problems associated with altitude-including instruments. A sample of reports from pilots and controllers is included, covering the topics of ATIS broadcasts an clearance readback problems. A selection of Alert Bulletins, with their responses, is included.

Source record↗

Integrated Advanced Microwave Sounding Unit-A (AMSU-A). Performance Verification Report: AMSU-A1 Antenna Drive Subsystem, PN 1331720-2, S/N 106

This is the Performance Verification Report, AMSU-A1 Antenna Drive Subsystem, P/N 1331720-2, S/N 106, for the Integrated Advanced Microwave Sounding Unit-A (AMSU-A). The antenna drive subsystem of the METSAT AMSU-A1, S/N 106, P/N 1331720-2, completed acceptance testing per A-ES Test Procedure AE-26002/lD. The test included: Scan Motion and Jitter, Pulse Load Bus Peak Current and Rise Time, Resolver Reading and Position Error, Gain/ Phase Margin, and Operational Gain Margin. The drive motors and electronic circuitry were also tested at the component level. The drive motor test includes: Starting Torque Test, Motor Commutation Test, Resolver Operation/ No-Load Speed Test, and Random Vibration. The electronic circuitry was tested at the Circuit Card Assembly (CCA) level of production; each test exercised all circuit functions. The transistor assembly was tested during the W3 cable assembly (1356941-1) test.

Luu, D.↗

Plant chlorophyll content meter

A plant chlorophyll content meter is described which collects light reflected from a target plant and separates the collected light into two different wavelength bands. These wavelength bands, or channels, are described as having center wavelengths of 700 nm and 840 nm. The light collected in these two channels are processed using photo detectors and amplifiers. An analog to digital converter is described which provides a digital representation of the level of light collected by the lens and falling within the two channels. A controller provided in the meter device compares the level of light reflected from a target plant with a level of light detected from a light source, such as light reflected by a target having 100% reflectance, or transmitted through a diffusion receptor. The percent of reflection in the two separate wavelength bands from a target plant are compared to provide a ratio which indicates a relative level of plant physiological stress. A method of compensating for electronic drift is described where a sample is taken when a collection lens is covered to prevent light from entering the device. This compensation method allows for a more accurate reading by reducing error contributions due to electronic drift from environmental conditions at the location where a hand-held unit is used.

Spiering, Bruce A.↗

Nonvolatile random access memory

A nonvolatile magnetic random access memory can be achieved by an array of magnet-Hall effect (M-H) elements. The storage function is realized with a rectangular thin-film ferromagnetic material having an in-plane, uniaxial anisotropy and inplane bipolar remanent magnetization states. The thin-film magnetic element is magnetized by a local applied field, whose direction is used to form either a 0 or 1 state. The element remains in the 0 or 1 state until a switching field is applied to change its state. The stored information is detcted by a Hall-effect sensor which senses the fringing field from the magnetic storage element. The circuit design for addressing each cell includes transistor switches for providing a current of selected polarity to store a binary digit through a separate conductor overlying the magnetic element of the cell. To read out a stored binary digit, transistor switches are employed to provide a current through a row of Hall-effect sensors connected in series and enabling a differential voltage amplifier connected to all Hall-effect sensors of a column in series. To avoid read-out voltage errors due to shunt currents through resistive loads of the Hall-effect sensors of other cells in the same column, at least one transistor switch is provided between every pair of adjacent cells in every row which are not turned on except in the row of the selected cell.

Wu, Jiin-Chuan↗

Coaxial Electric Heaters

Coaxial electric heaters have been conceived for use in highly sensitive instruments in which there are requirements for compact heaters but stray magnetic fields associated with heater electric currents would adversely affect operation. Such instruments include atomic clocks and magnetometers that utilize heated atomic-sample cells, wherein stray magnetic fields at picotesla levels could introduce systematic errors into instrument readings. A coaxial electric heater is essentially an axisymmetric coaxial cable, the outer conductor of which is deliberately made highly electrically resistive so that it can serve as a heating element. As in the cases of other axisymmetric coaxial cables, the equal magnitude electric currents flowing in opposite directions along the inner and outer conductors give rise to zero net magnetic field outside the outer conductor. Hence, a coaxial electric heater can be placed near an atomic-sample cell or other sensitive device. A coaxial electric heater can be fabricated from an insulated copper wire, the copper core of which serves as the inner conductor. For example, in one approach, the insulated wire is dipped in a colloidal graphite emulsion, then the emulsion-coated wire is dried to form a thin, uniform, highly electrically resistive film that serves as the outer conductor. Then the film is coated with a protective layer of high-temperature epoxy except at the end to be electrically connected to the power supply. Next, the insulation is stripped from the wire at that end. Finally, electrical leads from the heater power supply are attached to the exposed portions of the wire and the resistive film. The resistance of the graphite film can be tailored via its thickness. Alternatively, the film can be made from an electrically conductive paint, other than a colloidal graphite emulsion, chosen to impart the desired resistance. Yet another alternative is to tailor the resistance of a graphite film by exploiting the fact that its resistance can be changed permanently within about 10 percent by heating it to a temperature above 300 C. A coaxial heater, with electrical leads attached, that has been bent into an almost full circle for edge heating of a circular window is shown. (In the specific application, there is a requirement for a heated cell window, through which an optical beam enters the cell.)

Strekalov, Dmitry↗