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Histological preparation of developing vestibular otoconia for scanning electron microscopy

The unique nature of vestibular otoconia as calcium carbonate biominerals makes them particularly susceptible to chemical deformation during histological processing. We fixed and stored otoconia from all three otolith endorgans of embryonic, hatchling and adult Japanese quail in glutaraldehyde containing either phosphate or non-phosphate buffers for varying lengths of time and processed them for scanning electron microscopy. Otoconia from all age groups and otolith endorgans processed in 0.1 M phosphate buffer (pH 7.4) showed abnormal surface morphology when compared to acetone fixed controls. Otoconia processed in 0.1 M sodium cacodylate or HEPES buffered artificial endolymph (pH 7.4) showed normal morphology that was similar to controls. The degree of otoconial deformation was directly related to the time exposed to phosphate buffer. Short duration exposure produced particulate deformations while longer exposures resulted in fused otoconia that formed solid sheets. Otoconial surface deformation and fusing was independent of the glutaraldehyde component of the histological processing. These findings should help vestibular researchers to develop appropriate histological processing protocols in future studies of otoconia.

Non-NASA Center

Glass and Glass-Ceramic Materials from Simulated Composition of Lunar and Martian Soils: Selected Properties and Potential Applications

In-situ resource processing and utilization on planetary bodies is an important and integral part of NASA's space exploration program. Within this scope and context, our general effort is primarily aimed at developing glass and glass-ceramic type materials using lunar and martian soils, and exploring various applications of these materials for planetary surface operations. Our preliminary work to date have demonstrated that glasses can be successfully prepared from melts of the simulated composition of both lunar and martian soils, and the melts have a viscosity-temperature window appropriate for drawing continuous glass fibers. The glasses are shown to have the potential for immobilizing certain types of nuclear wastes without deteriorating their chemical durability and thermal stability. This has a direct impact on successfully and economically disposing nuclear waste generated from a nuclear power plant on a planetary surface. In addition, these materials display characteristics that can be manipulated using appropriate processing protocols to develop glassy or glass-ceramic magnets. Also discussed in this presentation are other potential applications along with a few selected thermal, chemical, and structural properties as evaluated up to this time for these materials.

Ray, C. S.

Obtaining Remote-Sensing Reflectance from Multiple Instrument Systems

Obtaining accurate in situ measurements of Apparent Optical Properties (AOPs) is critical to maintaining satellite data quality. One approach to ensure accuracy is to deploy several independent instruments to measure the same phenomenon. During a cruise in June 2012, off the lee coast of the island of Hawaii, repeated profiles were made with two separate radiometric systems, one from Satlantic, Inc. (Hyperpro) and the other from Biospherical Instruments, Inc. (C-Ops). The C-Ops is multispectral, while the Hyperpro is hyperspectral. Both measure above-water solar irradiance (E(sub s)), downwelling in-water irradiance (E(sub d)), and upwelling in-water radiance (L(sub u)). From these measurements remotely-sensed reflectance (R(sub rs))can be calculated and compared with satellite data. All instruments were calibrated shortly before use, and while differences are to be expected due to temporal changes and spectral weighting differences, these should be consistent and minimal. We explore these differences, and compare to data retrieved from the NASA Moderate Resolution Imaging Spectroradiometer onboard Aqua (MODIS Aqua) when available. We also examine data collection and processing protocols for these systems.

Carbon

The use of analogs in the innovation process - A software engineering protocol analysis

A human information processing account of how experienced practitioners utilize analogs in the innovation process is presented. The protocols of several practitioners are collected and analyzed. The problem behavior graphs, knowledge elements utilized, and operator elements applied are described, and a descriptive model of the innovation process is assembled. Implications for a production system, deviations from other models of the innovation process, and extensions to other models of the analogy procedure are explored.

Silverman, B. G.

Advanced information processing system: Authentication protocols for network communication

In safety critical I/O and intercomputer communication networks, reliable message transmission is an important concern. Difficulties of communication and fault identification in networks arise primarily because the sender of a transmission cannot be identified with certainty, an intermediate node can corrupt a message without certainty of detection, and a babbling node cannot be identified and silenced without lengthy diagnosis and reconfiguration . Authentication protocols use digital signature techniques to verify the authenticity of messages with high probability. Such protocols appear to provide an efficient solution to many of these problems. The objective of this program is to develop, demonstrate, and evaluate intercomputer communication architectures which employ authentication. As a context for the evaluation, the authentication protocol-based communication concept was demonstrated under this program by hosting a real-time flight critical guidance, navigation and control algorithm on a distributed, heterogeneous, mixed redundancy system of workstations and embedded fault-tolerant computers.

Harper, Richard E.

Recommendations for a service framework to access astronomical archives

There are a large number of astronomical archives and catalogs on-line for network access, with many different user interfaces and features. Some systems are moving towards distributed access, supplying users with client software for their home sites which connects to servers at the archive site. Many of the issues involved in defining a standard framework of services that archive/catalog suppliers can use to achieve a basic level of interoperability are described. Such a framework would simplify the development of client and server programs to access the wide variety of astronomical archive systems. The primary services that are supplied by current systems include: catalog browsing, dataset retrieval, name resolution, and data analysis. The following issues (and probably more) need to be considered in establishing a standard set of client/server interfaces and protocols: Archive Access - dataset retrieval, delivery, file formats, data browsing, analysis, etc.; Catalog Access - database management systems, query languages, data formats, synchronous/asynchronous mode of operation, etc.; Interoperability - transaction/message protocols, distributed processing mechanisms (DCE, ONC/SunRPC, etc), networking protocols, etc.; Security - user registration, authorization/authentication mechanisms, etc.; Service Directory - service registration, lookup, port/task mapping, parameters, etc.; Software - public vs proprietary, client/server software, standard interfaces to client/server functions, software distribution, operating system portability, data portability, etc. Several archive/catalog groups, notably the Astrophysics Data System (ADS), are already working in many of these areas. In the process of developing StarView, which is the user interface to the Space Telescope Data Archive and Distribution Service (ST-DADS), these issues and the work of others were analyzed. A framework of standard interfaces for accessing services on any archive system which would benefit archive user and supplier alike is proposed.

Travisano, J. J.

Packet telemetry and packet telecommand - The new generation of spacecraft data handling techniques

Because of rising costs and reduced reliability of spacecraft and ground network hardware and software customization, standardization Packet Telemetry and Packet Telecommand concepts are emerging as viable alternatives. Autonomous packets of data, within each concept, which are created within ground and space application processes through the use of formatting techniques, are switched end-to-end through the space data network to their destination application processes through the use of standard transfer protocols. This process may result in facilitating a high degree of automation and interoperability because of completely mission-independent-designed intermediate data networks. The adoption of an international guideline for future space telemetry formatting of the Packet Telemetry concept, and the advancement of the NASA-ESA Working Group's Packet Telecommand concept to a level of maturity parallel to the of Packet Telemetry are the goals of the Consultative Committee for Space Data Systems. Both the Packet Telemetry and Packet Telecommand concepts are reviewed.

Hooke, A. J.

Encryption for Remote Control via Internet or Intranet

A data-communication protocol has been devised to enable secure, reliable remote control of processes and equipment via a collision-based network, while using minimal bandwidth and computation. The network could be the Internet or an intranet. Control is made secure by use of both a password and a dynamic key, which is sent transparently to a remote user by the controlled computer (that is, the computer, located at the site of the equipment or process to be controlled, that exerts direct control over the process). The protocol functions in the presence of network latency, overcomes errors caused by missed dynamic keys, and defeats attempts by unauthorized remote users to gain control. The protocol is not suitable for real-time control, but is well suited for applications in which control latencies up to about 0.5 second are acceptable. The encryption scheme involves the use of both a dynamic and a private key, without any additional overhead that would degrade performance. The dynamic key is embedded in the equipment- or process-monitor data packets sent out by the controlled computer: in other words, the dynamic key is a subset of the data in each such data packet. The controlled computer maintains a history of the last 3 to 5 data packets for use in decrypting incoming control commands. In addition, the controlled computer records a private key (password) that is given to the remote computer. The encrypted incoming command is permuted by both the dynamic and private key. A person who records the command data in a given packet for hostile purposes cannot use that packet after the public key expires (typically within 3 seconds). Even a person in possession of an unauthorized copy of the command/remote-display software cannot use that software in the absence of the password. The use of a dynamic key embedded in the outgoing data makes the central-processing unit overhead very small. The use of a National Instruments DataSocket(TradeMark) (or equivalent) protocol or the User Datagram Protocol makes it possible to obtain reasonably short response times: Typical response times in event-driven control, using packets sized .300 bytes, are <0.2 second for commands issued from locations anywhere on Earth. The protocol requires that control commands represent absolute values of controlled parameters (e.g., a specified temperature), as distinguished from changes in values of controlled parameters (e.g., a specified increment of temperature). Each command is issued three or more times to ensure delivery in crowded networks. The use of absolute-value commands prevents additional (redundant) commands from causing trouble. Because a remote controlling computer receives "talkback" in the form of data packets from the controlled computer, typically within a time interval < or =1 s, the controlling computer can re-issue a command if network failure has occurred. The controlled computer, the process or equipment that it controls, and any human operator(s) at the site of the controlled equipment or process should be equipped with safety measures to prevent damage to equipment or injury to humans. These features could be a combination of software, external hardware, and intervention by the human operator(s). The protocol is not fail-safe, but by adopting these safety measures as part of the protocol, one makes the protocol a robust means of controlling remote processes and equipment by use of typical office computers via intranets and/or the Internet.

Lineberger, Lewis

Predicting Char Yield of High-Temperature Resins

A simulation protocol is developed to predict the char yield of organic resins during high-temperature processing. Simulation protocol in silico methods can help screen promising new formulations for advanced materials, but previously no chemistry-sensitive technique existed to predict the important experimental value of char yield. The method utilizes a reactive force field (ReaxFF) to model the chemical transformation of precursor monomers into carbonized structures during three processing stages: ramp-up to processing temperatures (~3000 K), pyrolysis, and quenching. Achieving good agreement with experimental char yields requires continuous removal of small byproduct molecules to mimic outgassing, and the application of high pressure to eliminate porosity and encourage graphitization. More than ten different resin chemistries are investigated, including arylacetylenes, cyanate esters, phthalonitriles, and polyimides, representing a diverse group of precursors with respect to initial cyclic content, heteroatoms and reactive groups. The protocol correctly predicts the relative char yield between the investigated chemistries and provides quantitative agreement with experimental values, especially for high char yield resins. The properties of the resins during processing are compared, including outgassing products, morphology of the final chemical configurations, cyclic content and mechanical properties.

polymer simulations, molecular dynamics

Mars Sample Handling Protocol Workshop Series: Workshop 4

In preparation for missions to Mars that will involve the return of samples to Earth, it will be necessary to prepare for the receiving, handling, testing, distributing, and archiving of martian materials here on Earth. Previous groups and committees have studied selected aspects of sample return activities, but specific detailed protocols for the handling and testing of returned samples must still be developed. To further refine the requirements for sample hazard testing and to develop the criteria for subsequent release of sample materials from quarantine, the NASA Planetary Protection Officer convened a series of workshops in 2000-2001. The overall objective of the Workshop Series was to produce a Draft Protocol by which returned martian sample materials can be assessed for biological hazards and examined for evidence of life (extant or extinct) while safeguarding the purity of the samples from possible terrestrial contamination. This report also provides a record of the proceedings of Workshop 4, the final Workshop of the Series, which was held in Arlington, Virginia, June 5-7, 2001. During Workshop 4, the sub-groups were provided with a draft of the protocol compiled in May 2001 from the work done at prior Workshops in the Series. Then eight sub-groups were formed to discuss the following assigned topics: Review and Assess the Draft Protocol for Physical/Chemical Testing Review and Assess the Draft Protocol for Life Detection Testing Review and Assess the Draft Protocol for Biohazard Testing Environmental and Health/Monitoring and Safety Issues Requirements of the Draft Protocol for Facilities and Equipment Contingency Planning for Different Outcomes of the Draft Protocol Personnel Management Considerations in Implementation of the Draft Protocol Draft Protocol Implementation Process and Update Concepts This report provides the first complete presentation of the Draft Protocol for Mars Sample Handling to meet planetary protection needs. This Draft Protocol, which was compiled from deliberations and recommendations from earlier Workshops in the Series, represents a consensus that emerged from the discussions of all the sub-groups assembled over the course of the five Workshops of the Series. These discussions converged on a conceptual approach to sample handling, as well as on specific analytical requirements. Discussions also identified important issues requiring attention, as well as research and development needed for protocol implementation.

Race Margaret S.

Field Immune Assessment during Simulated Planetary Exploration in the Canadian Arctic

Dysregulation of the immune system has been shown to occur during space flight, although the detailed nature of the phenomenon and the clinical risks for exploration class missions has yet to be established. In addition, the growing clinical significance of immune system evaluation combined with epidemic infectious disease rates in third world countries provides a strong rationale for the development of field-compatible clinical immunology techniques and equipment. In July 2002 NASA performed a comprehensive field immunology assessment on crewmembers participating in the Haughton-Mars Project (HMP) on Devon Island in the high Canadian Arctic. The purpose of the study was to evaluate mission-associated effects on the human immune system, as well as to evaluate techniques developed for processing immune samples in remote field locations. Ten HMP-2002 participants volunteered for the study. A field protocol was developed at NASA-JSC for performing sample collection, blood staining/processing for immunophenotype analysis, wholeblood mitogenic culture for functional assessments and cell-sample preservation on-location at Devon Island. Specific assays included peripheral leukocyte distribution; constitutively activated T cells, intracellular cytokine profiles and plasma EBV viral antibody levels. Study timepoints were L-30, midmission and R+60. The protocol developed for immune sample processing in remote field locations functioned properly. Samples were processed in the field location, and stabilized for subsequent analysis at the Johnson Space Center in Houston. The data indicated that some phenotype, immune function and stress hormone changes occurred in the HMP field participants that were largely distinct from pre-mission baseline and post-mission recovery data. These immune changes appear similar to those observed in Astronauts following spaceflight. The sample processing protocol developed for this study may have applications for immune assessment during exploration-class space missions or in remote terrestrial field locations. The data validate the use of the HMP as a ground-based spaceflight/planetary exploration analog for some aspects of human physiology.

Crucian, Brian

Vacillation Made Easy: Distribution, Re-distribution, and Un-distribution of DOPL-based Processing

Distributed Objects Protocol Layer, or DOPL, Provides a simple and general data communication abstraction that can support the distribution of C++ applications software functionality among an arbitrary collection of processors. The purposed of the abstraction is to minimize the cost of revising processing distribution decisions throughout the software development cycle, including after software has beed delivered to users.

Vacillation

Telecommunications end-to-end systems monitoring on TOPEX/Poseidon: Tools and techniques

The TOPEX/Poseidon Project Satellite Performance Analysis Team's (SPAT) roles and responsibilities have grown to include functions that are typically performed by other teams on JPL Flight Projects. In particular, SPAT Telecommunication's role has expanded beyond the nominal function of monitoring, assessing, characterizing, and trending the spacecraft (S/C) RF/Telecom subsystem to one of End-to-End Information Systems (EEIS) monitoring. This has been accomplished by taking advantage of the spacecraft and ground data system structures and protocols. By processing both the received spacecraft telemetry minor frame ground generated CRC flags and NASCOM block poly error flags, bit error rates (BER) for each link segment can be determined. This provides the capability to characterize the separate link segments, determine science data recovery, and perform fault/anomaly detection and isolation. By monitoring and managing the links, TOPEX has successfully recovered approximately 99.9 percent of the science data with an integrity (BER) of better than 1 x 10(exp 8). This paper presents the algorithms used to process the above flags and the techniques used for EEIS monitoring.

Calanche, Bruno J.

The NASA Integrated Information Technology Architecture

This document defines an Information Technology Architecture for the National Aeronautics and Space Administration (NASA), where Information Technology (IT) refers to the hardware, software, standards, protocols and processes that enable the creation, manipulation, storage, organization and sharing of information. An architecture provides an itemization and definition of these IT structures, a view of the relationship of the structures to each other and, most importantly, an accessible view of the whole. It is a fundamental assumption of this document that a useful, interoperable and affordable IT environment is key to the execution of the core NASA scientific and project competencies and business practices. This Architecture represents the highest level system design and guideline for NASA IT related activities and has been created on the authority of the NASA Chief Information Officer (CIO) and will be maintained under the auspices of that office. It addresses all aspects of general purpose, research, administrative and scientific computing and networking throughout the NASA Agency and is applicable to all NASA administrative offices, projects, field centers and remote sites. Through the establishment of five Objectives and six Principles this Architecture provides a blueprint for all NASA IT service providers: civil service, contractor and outsourcer. The most significant of the Objectives and Principles are the commitment to customer-driven IT implementations and the commitment to a simpler, cost-efficient, standards-based, modular IT infrastructure. In order to ensure that the Architecture is presented and defined in the context of the mission, project and business goals of NASA, this Architecture consists of four layers in which each subsequent layer builds on the previous layer. They are: 1) the Business Architecture: the operational functions of the business, or Enterprise, 2) the Systems Architecture: the specific Enterprise activities within the context of IT systems, 3) the Technical Architecture: a common, vendor-independent framework for design, integration and implementation of IT systems and 4) the Product Architecture: vendor=specific IT solutions. The Systems Architecture is effectively a description of the end-user "requirements". Generalized end-user requirements are discussed and subsequently organized into specific mission and project functions. The Technical Architecture depicts the framework, and relationship, of the specific IT components that enable the end-user functionality as described in the Systems Architecture. The primary components as described in the Technical Architecture are: 1) Applications: Basic Client Component, Object Creation Applications, Collaborative Applications, Object Analysis Applications, 2) Services: Messaging, Information Broker, Collaboration, Distributed Processing, and 3) Infrastructure: Network, Security, Directory, Certificate Management, Enterprise Management and File System. This Architecture also provides specific Implementation Recommendations, the most significant of which is the recognition of IT as core to NASA activities and defines a plan, which is aligned with the NASA strategic planning processes, for keeping the Architecture alive and useful.

Baldridge, Tim

Bio-Optical Measurement and Modeling of the California Current and Polar Oceans

The principal goals of our research are to validate standard or experimental products through detailed bio-optical and biogeochemical measurements, and to combine ocean optical observations with advanced radiative transfer modeling to contribute to satellite vicarious radiometric calibration and advanced algorithm development. To achieve our goals requires continued efforts to execute complex field programs globally, as well as development of advanced ocean optical measurement protocols. We completed a comprehensive set of ocean optical observations in the California Current, Southern Ocean, Indian Ocean requiring a large commitment to instrument calibration, measurement protocols, data processing and data merger. We augmented separately funded projects of our own, as well as others, to acquire ill situ data sets we have collected on various global cruises supported by separate grants or contracts. In collaboration with major oceanographic ship-based observation programs funded by various agencies (CalCOFI, US JGOFS, NOAA AMLR, INDOEX and Japan/East Sea) our SIMBIOS effort has resulted in data from diverse bio-optical provinces. For these global deployments we generate a high-quality, methodologically consistent, data set encompassing a wide-range of oceanic conditions. Global data collected in recent years have been integrated with our on-going CalCOFI database and have been used to evaluate SeaWiFS algorithms and to carry out validation studies. The combined database we have assembled now comprises more than 700 stations and includes observations for the clearest oligotrophic waters, highly eutrophic blooms, red-tides and coastal case 2 conditions. The data has been used to validate water-leaving radiance estimated with SeaWiFS as well as bio-optical algorithms for chlorophyll pigments. The comprehensive data is utilized for development of experimental algorithms (e.g. high-low latitude pigment transition, phytoplankton absorption, and cDOM). During this period we completed 9 peer-reviewed publications in high quality journals, and presented aspects of our work at more than 10 scientific conferences.

Mitchell, B. Greg

Implementations of Sensor Webs Utilizing Uninhabited Aerial Systems

In this paper we describe the web services, processes, communication protocols and ad-hoc service chains utilized in the late summer and early fall 2007 Ikhana UAS response to the wildfires burning in southern California. Additionally, we describe the lessons learned that will be applied to the upcoming Global Hawk UAS Aura Satellite Validation Experiment planned for early 2009.

Sullivan, Donald V.

Remotely Powered Reconfigurable Receiver for Extreme Environment Sensing Platforms

Wireless sensors connected in a local network offer revolutionary exploration capabilities, but the current solutions do not work in extreme environments of low temperatures (200K) and low to moderate radiation levels (<50 krad). These sensors (temperature, radiation, infrared, etc.) would need to operate outside the spacecraft/ lander and be totally independent of power from the spacecraft/lander. Flash memory field-programmable gate arrays (FPGAs) are being used as the main signal processing and protocol generation platform in a new receiver. Flash-based FPGAs have been shown to have at least 100 reduced standby power and 10 reduction operating power when compared to normal SRAM-based FPGA technology.

Sheldon, Douglas J.