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Cytochrome c(3) from Desulfovibrio vulgaris Miyazaki F was successfully expressed in the facultative aerobe Shewanella oneidensis MR-1 under anaerobic, microaerophilic, and aerobic conditions, with yields of 0.3 to 11.5 mg of cytochrome/g of cells.
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In June 1978, a joint Soviet-American project (JASPIC) was conducted, the goals of which were: (1) the study of the flows of spilling electrons which act upon the middle-latitude ionosphere under nocturnal conditions (nocturnal corpuscular source of ionization); and (2) the mutual comparison of procedures for recording corpuscular radiations in the upper atmosphere, using meteorological and geophysical rockets.
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Effective budget planning considers not only how much money a project requires, but also when the money is needed. Many times, project success isn t the result of not making mistakes; it s the result of having the courage to face mistakes head on and take action.
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The quality of volume-localized magnetic resonance spectroscopy is affected by eddy currents caused by gradient switching. Eddy currents can be reduced with improved gradient systems; however, it has been suggested that the distortion due to eddy currents can be compensated for during postprocessing with a single-frequency reference signal. The authors propose modifying current techniques for acquiring the single-frequency reference signal by using relaxation weighting to reduce interference from components that cannot be eliminated by digital filtering alone. Additional sequences with T1 or T2 weighting for reference signal acquisition are shown to have the same eddy current characteristics as the original signal without relaxation weighting. The authors also studied a new eddy current correction method that does not require a single-frequency reference signal. This method uses two free induction decays (FIDs) collected from the same volume with two sequences with opposite gradients. Phase errors caused by eddy currents are opposite in these two FIDs and can be canceled completely by combining the FIDs. These methods were tested in a phantom. Eddy current distortions were corrected, allowing quantitative measurement of structures such as the -CH = CH- component, which is otherwise undetectable.
OBJECTIVE: Many exercise protocols used in physiological studies assume homogeneous and diffuse muscle recruitment. To test this assumption during a "standard" wrist flexion protocol, variations in muscle recruitment were assessed using MRI in eight healthy subjects. MATERIALS AND METHODS: Variations were assessed by comparing the right to the left forearms and the effect of slight (15 degrees) pronation or supination at the wrist. RESULTS: Postexercise imaging showed focal regions of increased signal intensity (SI), indicating relatively strong recruitment, most often in entire muscles, although occasionally only in subvolumes of muscles. In 15 of 26 studies, flexor carpi radialis (FCR) showed more SI than flexor carpi ulnaris, while in 11 studies SI in these muscles increased equivalently. Relatively greater FCR recruitment was seen during pronation and/or use of the nondominant side. Palmaris longus, a wrist flexor, did not appear recruited in 4 of 11 forearms in which it was present. A portion of the superficial finger flexor became hyperintense in 89% of studies, while recruitment of the deep finger flexor was seen only in 43%. CONCLUSION: Inter- and intraindividual variations in forearm muscle recruitment should be anticipated in physiological studies of standard wrist flexion exercise protocols.
The JPL experiment is indeed similar in spirit to that of Cialdea, but not the earlier MIT experiments (which involved the comparison of laser cavities).
The Small Rocket/Spacecraft Technology (SMART) is an existing microsatellite prototype bus that provides the ability to accommodate focused science (and technology validation) missions without the need to spend large resources typical of high‐performing spacecraft. SMART represents the sum value of GSFC’s long tradition of developing systems that find efficient application in a series of missions, exemplified by two successful major programs: the Multi-mission Modular Spacecraft (MMS), and the Small Explorer Program (SMEX). SMART is based on an evolutionary architecture based drawn from these two experiences: the Modular, Reconfigurable, Rapid (MR2) architecture. This paper shows a SMART-derivative spacecraft (with re-sized structure) used to accommodate the Abundance of Methane via Interferometric Glint Observation (AMIGO) instrument. The principal objective of AMIGO is to map and quantify the abundance of the major greenhouse gases (particularly methane) and to identify and quantify their sources and sinks on a global basis. In addition, a Laser retro-reflector instrument of opportunity is included, to add to the constellation of spacecraft relevant to the definition and regular update of the International Terrestrial Reference Frame (ITRF).