Results for “information protection” — Page 2

NASA NTRSDate not supplied

Evaluating Rock Pool Hydroperiod Fluctuation using Climate Variables to Inform Habitat Monitoring and Protection in the Western Sonoran Desert

As warming and drying trends continue to impact the greater American Southwest, effective ecosystem management increasingly relies upon understanding the relationships between climate and water resources. Ephemeral freshwater rock pools, known as tinajas, have great ecological and cultural importance as some of the only sources of surface water in the western Sonoran Desert (WSD). Tinaja flooding and drying cycles, known as hydroperiods, vary based on meteorologic and climatologic conditions; however, a lack of extensive research relating climatic impacts to tinajas puts these critical ecosystems further at risk. Tinajas throughout the WSD are monitored by the National Park Service (NPS) using resource intensive strategies including in situ trail camera observation and direct measurement. To aid NPS monitoring efforts, this research used remotely sensed climate data to analyze spatiotemporal climate trends and relationships between climate variables and tinaja hydroperiods in the WSD between 1979–2022. Using Aqua and Terra Moderate Resolution Imaging Spectroradiometers (MODIS), University of Idaho Gridded Surface Meteorological Dataset (gridMET), and OpenET data, the project analyzed land surface temperature, evapotranspiration, precipitation, wind velocity, and solar radiation. The team generated climate anomaly time series and climate normal maps for the WSD, identifying statistically significant spatial and temporal trends. These data were then compared to daily, qualitative in situ hydroperiod observations taken between 2019–2022. This work will be used by the NPS to inform the monitoring and protection of tinajas in the WSD. Findings contribute to a limited body of research concerning climate and tinajas, which are often overlooked despite their disproportionate ecological importance.

Annie Britton↗

NASA NTRS2022

Western Sonoran Desert Water Resources: Evaluating Rock Pool Hydroperiod Fluctuation using Climate Variables to Inform Habitat Monitoring and Protection in the Western Sonoran Desert

Ephemeral freshwater rock pools, known as tinajas, have great biologic and cultural importance as sources of surface water in the western Sonoran Desert (WSD). Tinaja flooding and drying cycles, known as hydroperiods, vary based on meteorologic and climatologic conditions; however, a lack of extensive research relating climatic impacts to tinajas puts these critical ecosystems further at risk. The National Park Service (NPS) and the University of Arizona monitor the physical and ecological condition of tinajas in Organ Pipe Cactus National Monument (OPCNM), AZ, using resource-intensive strategies: in situ trail cameras and direct measurements. To aid monitoring efforts, the NASA DEVELOP team aimed to incorporate remote sensing into NPS strategies by analyzing spatiotemporal climate data and tinaja hydroperiods in OPCNM between 1979–2022. Using Aqua and Terra Moderate Resolution Imaging Spectroradiometers (MODIS), University of Idaho Gridded Surface Meteorological Dataset (gridMET), and OpenET data, the team generated climatology maps and time series for OPCNM. The team compared these data to daily in situ hydroperiod observations from the University of Arizona between 2019–2022. Climate maps and time series showed increases in temperature and solar radiation (p<0.05), while analyses of in situ data showed correlations of hydroperiods with precipitation and evapotranspiration. End products identified high-risk tinajas and demonstrated that Earth observations can successfully be correlated with in situ hydroperiod observations. These results will support NPS efforts to prioritize water resource management and inform protocols driving the conservation of tinajas in OPCNM.

Anne Britton↗

DOE OSTI2026

Carbon-Based Quantum Information Science with Symmetry Protected Topological States (Final Report, DOE-BES award DE-SC0023105)

This research program established the scientific foundation for the rational, bottom-up design, synthesis, isolation, and investigation of symmetry-protected topological (SPT) electron spin qubits embedded in graphene nanoribbons (GNRs). The work focused on integrating atomically precise low-dimensional carbon nanostructures with emerging quantum logic architectures, providing a pathway toward scalable quantum materials for next-generation computing and sensing technologies. A central component of the program was the elucidation of fundamental relationships between real-space molecular architecture, local spin density distributions, electronic band dispersion, and energy level alignment in atomically precise GNR systems. These correlations define key operational parameters of SPT qubits and were systematically investigated to establish quantitative benchmarks against established molecular and solid-state spin qubit platforms. Attention was given to properties critical for quantum device performance, e.g. decoherence times, spectral sharpness of energy transitions, and tunable exchange interactions between spin states. The research demonstrated that these parameters can be engineered with atomic precision through scalable bottom-up synthetic strategies. Theory-guided design played a central role in identifying candidate structures hosting topologically protected spin states. Experimental validation was performed using both ensemble measurements and single-molecule characterization. In addition to advances in quantum materials synthesis, the program developed and applied spin-sensitive scanning probe microscopy techniques capable of directly probing quantum states and dynamic processes with atomic-scale spatial resolution. These capabilities enabled direct observation and characterization of quantum structures at the single-atom level. While the research activities were primarily hypothesis-driven fundamental investigations, the program adopted a comprehensive materials-by-design framework aimed at translating scientific discoveries into technological concepts compatible with scalable and intelligent manufacturing approaches.

36 MATERIALS SCIENCE↗

NASA NTRSDate not supplied

Evaluating Rock Pool Hydroperiod Fluctuation using Climate Variables to Inform Habitat Monitoring and Protection in the Western Sonoran Desert

Explore the source record for details and available documents.

Katie Lange↗

NASA NTRS1989

The sharing of rights and information in a capability-based protection system

The question of sharing of rights and information in the Take-Grant Protection Model is examined by concentrating on the similarities between the two; in order to do this, new theorems are stated and proven for each that specifically show the similarities. The proof for one of the original theorems is also provided. These statements of necessary and sufficient conditions are contrasted to illustrate the proposition that transferring rights and transferring information are fundamentally the same, as one would expect in a capability-based system. Directions are then discussed for future research in light of these results.

Bishop, Matt↗

NASA NTRS2001

NASA Fire Protection

This viewgraph presentation provides information on fire protection operations and administration at Stennis Space Center (SSC). The presentation also lists innovative practices and recent improvements.

Clark, Theodore↗

NASA NTRS2016

Tailoring NIST Security Controls for the Ground System: Selection and Implementation -- Recommendations for Information System Owners

The National Aeronautics and Space Administration (NASA) invests millions of dollars in spacecraft and ground system development, and in mission operations in the pursuit of scientific knowledge of the universe. In recent years, NASA sent a probe to Mars to study the Red Planet's upper atmosphere, obtained high resolution images of Pluto, and it is currently preparing to find new exoplanets, rendezvous with an asteroid, and bring a sample of the asteroid back to Earth for analysis. The success of these missions is enabled by mission assurance. In turn, mission assurance is backed by information assurance. The information systems supporting NASA missions must be reliable as well as secure. NASA - like every other U.S. Federal Government agency - is required to manage the security of its information systems according to federal mandates, the most prominent being the Federal Information Security Management Act (FISMA) of 2002 and the legislative updates that followed it. Like the management of enterprise information technology (IT), federal information security management takes a "one-size fits all" approach for protecting IT systems. While this approach works for most organizations, it does not effectively translate into security of highly specialized systems such as those supporting NASA missions. These systems include command and control (C&C) systems, spacecraft and instrument simulators, and other elements comprising the ground segment. They must be carefully configured, monitored and maintained, sometimes for several years past the missions' initially planned life expectancy, to ensure the ground system is protected and remains operational without any compromise of its confidentiality, integrity and availability. Enterprise policies, processes, procedures and products, if not effectively tailored to meet mission requirements, may not offer the needed security for protecting the information system, and they may even become disruptive to mission operations. Certain protective measures for the general enterprise may not be as efficient within the ground segment. This is what the authors have concluded through observations and analysis of patterns identified from the various security assessments performed on NASA missions such as MAVEN, OSIRIS-REx, New Horizons and TESS, to name a few. The security audits confirmed that the framework for managing information system security developed by the National Institute of Standards and Technology (NIST) for the federal government, and adopted by NASA, is indeed effective. However, the selection of the technical, operational and management security controls offered by the NIST model - and how they are implemented - does not always fit the nature and the environment where the ground system operates in even though there is no apparent impact on mission success. The authors observed that unfit controls, that is, controls that are not necessarily applicable or sufficiently effective in protecting the mission systems, are often selected to facilitate compliance with security requirements and organizational expectations even if the selected controls offer minimum or non-existent protection. This paper identifies some of the standard security controls that can in fact protect the ground system, and which of them offer little or no benefit at all. It offers multiple scenarios from real security audits in which the controls are not effective without, of course, disclosing any sensitive information about the missions assessed. In addition to selection and implementation of controls, the paper also discusses potential impact of recent legislation such as the Federal Information Security Modernization Act (FISMA) of 2014 - aimed at the enterprise - on the ground system, and offers other recommendations to Information System Owners (ISOs).

GOVERNANCE↗

NASA NTRS1996

The Influence of Future Command, Control, Communications, and Computers (C4) on Doctrine and the Operational Commander's Decision-Making Process

Future C4 systems will alter the traditional balance between force and information, having a profound influence on doctrine and the operational commander's decision making process. The Joint Staff's future vision of C4 is conceptualized in 'C4I for the Warrior' which envisions a joint C4I architecture providing timely sensor to shoot information direct to the warfighter. C4 system must manage and filter an overwhelming amount of information; deal with interoperability issues; overcome technological limitations; meet emerging security requirements; and protect against 'Information Warfare.' Severe budget constraints necessitate unified control of C4 systems under singular leadership for the common good of all the services. In addition, acquisition policy and procedures must be revamped to allow new technologies to be fielded quickly; and the commercial marketplace will become the preferred starting point for modernization. Flatter command structures are recommended in this environment where information is available instantaneously. New responsibilities for decision making at lower levels are created. Commanders will have to strike a balance between exerting greater control and allowing subordinates enough flexibility to maintain initiative. Clearly, the commander's intent remains the most important tool in striking this balance.

Mayer, Michael G.↗

DOE OSTI2025

Regulatory Considerations for Domestic Reprocessing Facility Physical Security

U.S. advanced non-light-water reactor vendors may pursue collocated on-site reprocessing activities. Therefore, these facilities are likely to possess formula quantities, or Category I quantities, of special nuclear material (SNM) during normal operations. The U.S. Nuclear Regulatory Commission (U.S. NRC) has yet to formally establish a regulatory framework for commercial reprocessing. While Category I requirements would explicitly not apply in this circumstance under current regulatory requirements, regulatory certainty does not exist. A novel framework should be developed to ensure public health and safety while also risk-informing the physical security requirements. This report reviews the relevant background of related rulemaking activities and proposes risk-informed physical protection requirements to satisfy these objectives. Insights from NRC security-related rulemaking activities provide a substantial technical basis to approach potential establishment of physical security requirements for reprocessing facilities. If a licensee can provide justification that the material satisfies a sufficient self-protecting radiation dose threshold, the material may not be subject to theft or diversion requirements and only potential sabotage requirements would apply. Furthermore, if the material can be justified to be moderately dilute, a set of risk-informed requirements could provide adequate protection of public health and safety. A revised performance objective for prevention of theft of moderately dilute Category I SNM may be detection to allow prompt recovery by a local law enforcement agency. However, a significant caveat to the proposed categorization scheme is the unknown integration of radiological sabotage with requirements for the protection against theft. Future licensees should consult with the NRC regarding treatment of this regulatory topic. Additionally, the self-protecting radiation dose threshold (either the existing or a proposed future threshold) would need to be considered. An integrated approach may apply graded potential requirements for protection against the design basis threat of radiological sabotage currently applicable to commercial nuclear power plants and Category I SNM facilities defined within 10 CFR 73.1(a).

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

NASA NTRS1997

Threatened and Endangered Species Survey for Patrick Air Force Base, Florida

A review of previous environmental work conducted at Patrick Air Force Base (PAFB) indicated that several threatened, endangered, or species of special concern occurred or had the potential to occur there. This study was implemented to collect more information on protected species at PAFB. A map of landcover types was prepared for PAFB using aerial photography, groundtruthing, and a geographic information system (GIS). Herbaceous vegetation was the most common vegetation type. The second most abundant vegetation type was disturbed shrubs/exotics. The beach and associated dune vegetation comprised 3.2% of the land area, but was the most extensive natural community within PAFB. A few isolated mangrove communities exist along the Banana River. Seventy-seven species of vascular plants occurred on the dunes, including four species listed by state agencies: spider lily (Hymenocallis latifolia), prickly pear cactus (Opuntia stricta), beach star (Remirea maritima), and inkberry (Scaevola plumien). Surveys of other habitats revealed eighty-four species of vascular plants including two state-listed species: spider lily and prickly pear cactus. Many of these areas are dominated by invasive, exotic species, particularly Brazilian pepper (Schinus terebinthifolius) and Australian pine (Casuarina equisetifolia), and native species of open or disturbed sites such as camphorweed (Heterotheca subaxillaris) and beardgrass (Andropogon spp.). Due to the isolation of PAFB from other natural areas, most exotic plant populations on the base are not an immediate threat to intact native plant communities. Dune habitat was surveyed for the southeastem beach mouse (Peromyscus polionotus niveiventris) by quarterly trapping along eight 100 m transects. No beach mice were found. The limited extent of dune habitat, its fragmented condition, and the isolation of PAFB from extant populations of the beach mouse probably accounts for its absence. Surveys of birds on PAFB found an avifauna characteristic of species that occur in the Indian River Lagoon system. Twenty-five species of waterbirds were observed during quarterly surveys on PAFB, including five species listed as species of special concern by the state of Florida: Snowy Egret (Egretta thula), Little Blue Heron (Egretta caerulea), Tricolored Heron (Egretta tricolo4, White Ibis (Eudocimus albus), and Brown Pelican (Pelecanus occidentalis). The Golf Course was used extensively by almost all species of waterbirds on PAFB. Twenty-two species of shorebirds were observed on PAFB. Although no listed species were observed, the potential exists for several protected species of shorebirds to use the beach at PAFB during some parts of the year. The Airfield runways and associated grass areas were important sites at PAFB for loafing and feeding for some shorebirds. Surveys of rooftop nesting by Least Terns (Stema antillarum) on PAFB found a large colony on a rooftop in the PAFB Industrial Area. This colony produced some independent young. Two rooftop Least Tern colonies reported from previous years were inactive during 1996. A small number of Black Skimmers (Rhynchops nigee attempted to nest at the Least Ten colony but were unsuccessful. Surveys for the gopher tortoise (Gopherus polyphemus) revealed burrows and tortoises only at the Waste Study Site; five burrows and three tortoises were observed. No Florida scrub lizards (Sceloporus woodi), eastern indigo snakes (Drymarchon corais couperl), or diamondback terrapins (Malademys terrapin terrapin) were observed. American alligators (Alligator mississippiensis) were observed on the Golf Course and using ditches, ponds, and areas along the Banana River. The amount of dune habitat could be expanded by not mowing areas adjacent to the dunes to allow dune species to colonize and expand. Planting dune species as part of the beach renourishment project will also increase this habitat. Exotic plants dominate several areas on the base and are used by threatened, endangered, and species of special concern. However, the use of native vegetation in landscaping projects throughout the base would improve habitat for wildlife, and invasive, exotic plants should not be used in any horticultural plantings. Water quality of ponds, ditches, and canals is important for waterbirds; it should be maintained and protected from contamination. Nesting Least Terns are sensitive to disturbance; rooftops used for nesting should be protected from disturbance. Monitoring of Least Tern and Black Skimmer nesting should be continued to determine what roofs are being used and whether nesting is successful. Furthermore, based on the large numbers of waterbirds observed on PAFB, continued monitoring of them is recommended.

Oddy, Donna M.↗

DOE OSTI2026

Differential Privacy in Grid Kitchen: Implementation & Software Documentation

Sharing of power grid feeder models faces significant challenges due to the potential risk of exposing sensitive operational information. Traditional anonymization techniques have shown notable limitations in other sensitive domains, as evidenced by documented re-identification attacks that combine supposedly anonymized datasets with auxiliary information, raising concerns that similar vulnerabilities could affect power grid data. Consequently, there is a pressing need for a more rigorous privacy protection strategy that not only delivers formal mathematical guarantees but also preserves the analytical value of the shared models. To address this challenge, we have enhanced the Grid Kitchen framework by implementing differential privacy mechanisms within the distribution model dehydration pipeline. This implementation carefully calibrates and applies noise to sensitive attributes in feeder models according to configurable privacy levels—low, moderate, and high—each offering different balances between data utility and privacy protection. Our approach uses established noise functions (Gaussian for continuous data and Discrete Laplace for integer values) with parameters carefully calibrated so that the impact of individual data points is effectively masked in the final output. The integration leverages our Noise Catalog, which we developed to categorize feeder model properties by component type, data type, and sensitivity. This catalog guides the application of appropriate noise functions and privacy parameters ($\varepsilon$ and $\delta$) to each attribute, ensuring consistent privacy protection across the model while maintaining its structural integrity and analytical usefulness. This implementation also includes evaluation tools that allow model owners to assess the impact of privacy-preserving transformations before sharing data with external parties. This report provides documentation for the differential privacy capabilities added to the Grid Kitchen project. It includes a primer on differential privacy concepts and their importance in modern data sharing, details the architecture of our implementation, explains the privacy modes and parameter configurations, and offers practical guidance on using the code for applying differential privacy to grid feeder models. Through examples and code snippets, we demonstrate the effective application of these privacy-enhancing technologies, enabling utility operators and researchers to confidently share grid data while protecting sensitive information.

24 POWER TRANSMISSION AND DISTRIBUTION↗

NASA NTRS2013

Advanced Planetary Protection Technologies for the Proposed Future Mission Set

Planetary protection is the discipline of protecting solar system objects from harmful contamination resulting from the activities of interplanetary spacecraft, and of similarly protecting the Earth from uncontrolled release of a putative extra-terrestrial organism from returned extra-terrestrial samples. Planetary protection requirements for Mars are becoming further refined as more is understood about the nature of the Martian environment as a potential habitat. Likewise, increased understanding of the limits of life on Earth is informing planetary protection policy. This presentation will discuss recent technology developments, ongoing work and future challenges of implementing planetary protection for the proposed future mission set.

Europa↗

NASA NTRS1986

Shuttle/spacelab contamination environment and effects handbook

This handbook is intended to assist users of the Spacelab/Space Transportation System by providing contamination environments and effects information that may be of value in planning, designing, manufacturing, and operating a space flight experiment. A summary of available molecular and particulate contamination data on the Space Transportation System and its facilities is presented. Contamination models, contamination effects, and protection methods information are also presented. In addition to contamination, the effects of the space environments at STS altitudes on spacecraft materials are included. Extensive references, bibliographies, and contacts are provided.

Bareiss, L. E.↗

NASA NTRSDate not supplied

ISS External Microorganisms: Tools and Techniques for Collecting Planetary Protection Samples During Extravehicular Activity

We have designed, built, and tested a sampling kit (Fig. 1) to aseptically collect microbiological samples from exterior surfaces on the ISS (International Space Station). The kit was flown to ISS as part of the NG-19 commercial cargo mission in August of 2023. Astronauts will use the kit to collect samples from six exterior surfaces on ISS. These samples will be frozen at -80°C after collection and returned to the ground for next generation DNA sequencing. The results of this experiment will help inform planetary protection requirements for crewed missions. We hypothesize that there are detectable microbial communities outside ISS and that these communities originate from inside ISS. Current life support systems do not include any components for reducing microbial leakage. Gases are vented from inside ISS without filtration and there are currently no protocols in place to minimize bioburden on space suit exteriors prior to use. It is important to quantify the bioburden on current vehicles so that achievable limits can be set for crewed missions to astrobiologically relevant locations like Mars.

Planetary Protection↗

NASA NTRSDate not supplied

ISS External Microorganisms: Collecting Planetary Protection Samples During Extravehicular Activity

Introduction: We have designed1, built, and tested a sampling kit (Fig. 1) to aseptically collect microbiological samples from exterior surfaces on the ISS (International Space Station). The kit was flown to ISS as part of the NG-19 commercial cargo mission in August of 2023. Astronauts will use the kit to collect samples from six exterior surfaces on ISS. These samples will be frozen at -80°C after collection and returned to the ground for next generation DNA sequencing. The results of this experiment will help inform planetary protection requirements for crewed missions. We hypothesize that there are detectable microbial communities outside ISS and that these communities originate from inside ISS. Current life support systems do not include any components for reducing microbial leakage. Gases are vented from inside ISS without filtration and there are currently no protocols in place to minimize bioburden on space suit exteriors prior to use. It is important to quantify the bioburden on current vehicles so that achievable limits can be set for crewed missions to astrobiologically relevant locations like Mars. Figure 1: The sampling kit contains eight swab canisters in total (six on the top and two on the bottom). A resuable end effector (1) is used to remove and reinstall the swabs. The kit also contains a handrail (2), tether loop (3), and, bayonet probes(4) to allow easy manipulation during EVA. Methods: We have designed a kit to carry 8 commercially available foam swabs into and out of vacuum without compromising the swabs’ sterility. The specially designed swab canisters contain a 0.2 µm Teflon filter that allows the canister to accommodate pressure changes without introducing unwanted contaminants. The kit meets existing EVA (Extravehicular Activity) safety requirements and has been used by human test subjects in the neutral buoyance lab and at vacuum in test chambers at the Johnson Space Center. In the early part of 2024, astronauts will use this sampling kit to swab six surfaces on the exterior of ISS. We will collect samples from: the airlock vestibule, the interior surface of the airlock thermal cover, an exterior handrail, a vent connected to the CO2 removal system, and a vent connected to the payload vacuum system inside ISS. Approximately 300 cm2 will be swabbed at each location. A seventh swab will be exposed to the vacuum of space without touching any surfaces as a blank. The eighth swab will remain sealed as a process control. After the EVA the swab kit will be frozen at -80°C and returned to earth at the earliest possible opportunity. The samples will remain frozen until they are thawed for next generation DNA sequencing on earth. We will use amplicon sequencing to identify any bacteria, archaea or fungi present in the samples. If there is enough DNA present, we will use shotgun metagenomic sequencing to further characterize the microbial ecology outside ISS. Preliminary Results: Results from ground-based testing demonstrate that the swab kit is capable of cycling in and out of vacuum without contaminating the swabs. Test subjects, wearing flight-like EVA gloves could remove swabs from the canister and sample discrete locations without inadvertently touching any other surfaces. Several types of bacteria and fungi were collected during these ground tests and survived up to 6 hours at vacuum. This includes non-spore forming bacterial like Staphyloccus capitis that are not traditionally considered extremophiles. Shotgun metagenomic sequencing of the ground-test samples revealed bacteria associated with human skin and airways on the exterior of space suits used during these tests 2 In addition to these results we will present preliminary results from our space-flight samples. We will also present lessons learned from attempting to collect microbiological samples during an EVA and describe how our results will affect planetary protection requirements for future crewed missions.

Aaron B. Regberg↗

NASA NTRSDate not supplied

ISS External Microorganisms: Collecting Planetary Protection Samples During Extravehicular Activity

Introduction: We have designed1, built, and tested a sampling kit (Fig. 1) to aseptically collect microbiological samples from exterior surfaces on the ISS (International Space Station). The kit was flown to ISS as part of the NG-19 commercial cargo mission in August of 2023. Astronauts will use the kit to collect samples from six exterior surfaces on ISS. These samples will be frozen at -80°C after collection and returned to the ground for next generation DNA sequencing. The results of this experiment will help inform planetary protection requirements for crewed missions. We hypothesize that there are detectable microbial communities outside ISS and that these communities originate from inside ISS. Current life support systems do not include any components for reducing microbial leakage. Gases are vented from inside ISS without filtration and there are currently no protocols in place to minimize bioburden on space suit exteriors prior to use. It is important to quantify the bioburden on current vehicles so that achievable limits can be set for crewed missions to astrobiologically relevant locations like Mars. Figure 1: The sampling kit contains eight swab canisters in total (six on the top and two on the bottom). A resuable end effector (1) is used to remove and reinstall the swabs. The kit also contains a handrail (2), tether loop (3), and, bayonet probes(4) to allow easy manipulation during EVA. Methods: We have designed a kit to carry 8 commercially available foam swabs into and out of vacuum without compromising the swabs’ sterility. The specially designed swab canisters contain a 0.2 µm Teflon filter that allows the canister to accommodate pressure changes without introducing unwanted contaminants. The kit meets existing EVA (Extravehicular Activity) safety requirements and has been used by human test subjects in the neutral buoyance lab and at vacuum in test chambers at the Johnson Space Center. In the early part of 2024, astronauts will use this sampling kit to swab six surfaces on the exterior of ISS. We will collect samples from: the airlock vestibule, the interior surface of the airlock thermal cover, an exterior handrail, a vent connected to the CO2 removal system, and a vent connected to the payload vacuum system inside ISS. Approximately 300 cm2 will be swabbed at each location. A seventh swab will be exposed to the vacuum of space without touching any surfaces as a blank. The eighth swab will remain sealed as a process control. After the EVA the swab kit will be frozen at -80°C and returned to earth at the earliest possible opportunity. The samples will remain frozen until they are thawed for next generation DNA sequencing on earth. We will use amplicon sequencing to identify any bacteria, archaea or fungi present in the samples. If there is enough DNA present, we will use shotgun metagenomic sequencing to further characterize the microbial ecology outside ISS. Preliminary Results: Results from ground-based testing demonstrate that the swab kit is capable of cycling in and out of vacuum without contaminating the swabs. Test subjects, wearing flight-like EVA gloves could remove swabs from the canister and sample discrete locations without inadvertently touching any other surfaces. Several types of bacteria and fungi were collected during these ground tests and survived up to 6 hours at vacuum. This includes non-spore forming bacterial like Staphyloccus capitis that are not traditionally considered extremophiles. Shotgun metagenomic sequencing of the ground-test samples revealed bacteria associated with human skin and airways on the exterior of space suits used during these tests 2 In addition to these results we will present preliminary results from our space-flight samples. We will also present lessons learned from attempting to collect microbiological samples during an EVA and describe how our results will affect planetary protection requirements for future crewed missions.

Aaron B. Regberg↗

NASA NTRSDate not supplied

Yeast Strain Development and Hardware Testing in Preparation of a Lunar BioSensor

With Artemis missions underway, it is clear we are going back to the Moon to stay. Before sending Astronauts for long-duration missions, it is crucial to understand the technological and biomedical countermeasures needed to protect them before they get there. We can use knowledge gained from biological CubeSats to guide the next generation of experiments to support human habitation on the Moon. Lunar Explorer Instrument for space biology Applications (LEIA) is NASA’s latest BioSensor, adapted BioSentinel, the only CubeSat to travel Beyond Low Earth Orbit. BioSentinel launched on Artemis I and is currently >50 million kilometers from Earth (as of July 2024). LEIA aims to identify biological responses to the Lunar environment, which unprotected against would pose a threat to astronauts (cancer, cardiovascular disease, neurological impairment). The suite of instruments within LEIA detects Lunar radiation using two on-board radiation sensors (ARES charged particle detector, Mini-Fast Neutron Detector), then monitors real-time biological responses to the Lunar environment via an autonomous microfluidic system, fit with 3-LED emitter and detector boards and the alamarBlue metabolic indicator dye. LEIA will use a genetic approach in addition to synthetic biology to test counter-measure production in space, with the goal to inform and protect astronauts for future Moon missions. We have conducted preliminary tests in preparation for launch to the anticipated South Pole of the Moon, optimizing the biology (strain down-selection, desiccation tolerance, radiation sensitivity) and improving the hardware (including a blue LED to detect the beta-carotene countermeasure product). Our team will discuss these findings in several parts – an overview of the LEIA mission (Mark Settles), adapting flexible CubeSat platforms for deep-space applications (Sergio Santa Maria, Kira Rienecker), developing new technologies to support LEIA ground studies (Chinmayee Govinda Raj), and yeast strain development and hardware testing in preparation for LEIA (presented here).

synthetic biology↗

DOE OSTI2025

Superselection rules, quantum error correction, and quantum chromodynamics

We investigate the relationship between superselection rules and quantum error correcting codes. We demonstrate that the existence of a superselection rule implies the Knill-Laflamme condition in quantum error correction. As an example, we examine the code built from quantum chromodynamics, where the proton and neutron states in the model are explored as different superselection sectors that protect logical information. Finally we comment on topological quantum error correcting codes and supersymmetric quantum field theory within this framework.

97 MATHEMATICS AND COMPUTING↗