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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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SPRUCE Methane Transport in Plants at S1 Bog, Marcell Experimental Forest, Minnesota, 2017-2019

This data set contains measurements of methane (CH4) transport by plants (both ground-layer and trees) and diffusion, as well as whole-plot emissions, taken in September 2018 and June 2019 in S1 Bog outside of the SPRUCE (Spruce and Peatland Responses Under Changing Environments) experimental enclosures. Additionally, CH4 and carbon dioxide (CO2) stable isotope data in porewater and atmospheric emissions were taken in July 2017 in the SPRUCE enclosures to explore the relative magnitude of CH4 oxidation. Episodic ebullition rates for S1 Bog are taken from Gill et al. (2017). Methane transport is an important component of many ecosystem models of peatlands. The results were compared to two methane models that have been developed for the SPRUCE project, ELM-SPRUCE (Earth Land Model) and TECO_SPRUCE (Terrestrial ECOsystem model). This dataset contains six data files in comma separate (.csv) format. Additional metadata are provided: six data dictionaries and a file-level metadata file in comma separate (.csv) format and a user guide in PDF (*.pdf) format.

54 ENVIRONMENTAL SCIENCES

SPRUCE Whole Ecosystem Warming (WEW) Environmental Data and Water Table Summaries, Marcell Experimental Forest, Minnesota, 2015-2024

This data set contains observations of photosynthetically active radiation (PAR), precipitation, soil temperature, soil volumetric water content, air temperature, relative humidity, and normalized water table depth that are summarized on a daily, weekly, monthly, and annual basis for each of the SPRUCE plots. Observations span 2015-2024. This dataset draws on several datasets (Hanson et al. 2016; Hanson et al. 2020; and Warren, unpublished data) and compiles these environmental observations into useful formats for data analysis. These environmental metrics can be used to understand the environmental conditions inside SPRUCE environmental chambers throughout the durations of the experiment and can be paired with other data for modeling and analysis. R code used to generate these files is provided as part of the data package. This dataset contains four data files in comma separate (.csv) format and a compressed folder (*.zip) containing three R (*.r) scripts. Additional metadata are provided: one data dictionary and a file-level metadata file in comma separate (.csv) format and a user guide in PDF (*.pdf) format. User note: Users must cite the original dataset/s along with this dataset when publishing any analyses using this dataset. Details on the dataset used to compile each variable are available in the header row of the files and in the user guide.

air temperature

SPRUCE Surface N2O fluxes measured with LI-7820, 2024

This dataset contains N2O (nitrous oxide) efflux rates measurements from the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experimental site within the Marcell Experimental Forest in northern Minnesota, USA. Measurements were made manually with a LiCor N2O/H2O analyzer (LI-7820) and paired SmartChamber (LI-8200-01S) in June, August, and October (2024-06-24 to 2024-10-22). During each measurement, the SmartChamber was placed on 8” PVC collars that were installed in May 2024. N2O flux was derived from 10-minute flux measurements processed using SoilFluxPro software (v5.3.1) and fit to a linear model. Model slope and R2 are reported along with soil water, soil temperature, and air temperature observations made with SmartChamber sensors. N2O is a gaseous N species formed during the microbial processes of denitrification and ammonia oxidation, and is a powerful greenhouse gas. This dataset contains one data file in comma-separate values (*.csv) format. Additional metadata are provided: one data dictionary and a file-level metadata file in comma-separate values (.csv) format and a user guide in PDF (*.pdf) format.

54 ENVIRONMENTAL SCIENCES

SPRUCE Aboveground Vegetation Coverage in Root Ingrowth Core Plots, Marcell Experimental Forest, Minnesota, August 2022

This dataset contains vegetation survey measurements from root ingrowth core plots (Määttä et al. 2025) inside SPRUCE Experiment plots at the Marcell Experimental Forest in northern Minnesota. Vegetation surveys were conducted in 0.25 meter2 plots containing root ingrowth cores on August 8th and 9th, 2022 (2022-08-08 to 2022-08-09). The warming and elevated carbon dioxide (CO2) treatments in the dataset include the full treatment gradient: +0 degrees Celsius (C) (+0 and +500 parts per million (ppm) elevated CO2), +2.25 degrees C (+0 and +500 ppm), +4.5 degrees C (+0 and +500 ppm elevated CO2), +6.75 degrees C (+0 and +500 ppm) and +9 degrees C (+0 and +500 ppm elevated CO2) for both hummocks and hollows. This dataset includes measurements of the height and absolute coverage (%) for each vascular plant and moss species, as well as organic litter and dead overstory vascular plants, and the distance from the grid center to the nearest tree and the species of the nearest tree. These data were used as species-specific aboveground plant metadata for assessing the warming and elevated CO2 response of fine roots across different peatland microtopographical features (hummocks and hollows) and plant functional types (shrub, spruce and larch). This dataset contains one data file in comma separate (.csv) format. Additional metadata are provided: one data dictionary and a file-level metadata file in comma separate (.csv) format and a user guide in PDF (*.pdf) format.

ESS-DIVE CSV File Formatting Guidelines Reporting

SPRUCE Redox-Active Subsurface Organic Matter, Marcell Experimental Forest, Minnesota, 2023

This dataset contains measurements that report on the effects of the SPRUCE experimental treatments on redox-active organic matter (RAOM) reduction (Valenzuela and Cervantes, 2021). Measurements occurred at the SPRUCE Experiment site in the Marcell Experimental Forest in northern Minnesota, USA. This work is also a follow-up to Rush et al. (2021a) which investigated effects of temperature on RAOM reduction after two years of experimental warming (Rush et al. 2021b). This follow-up dataset addresses two main questions; (i) How does warming and elevated carbon dioxide (CO2) directly affect in situ RAOM reduction, and subsequent methane (CH4) and CO2 production, across the peat depth profile? and (ii) How has long-term warming and elevated CO2 changed the total RAOM pool, and subsequent CH4 and CO2 production, across the peat depth profile? This dataset reports electron shuttling capacity (a proxy for RAOM reduction; Keller, and Takagi, 2013) and carbon dioxide (CO2) and methane (CH4) concentrations both in one-week in situ incubations (2023-05-31 to 2023-08-01) and 42-day laboratory incubations from peat collected in 2023 (2023-05-31 to 2023-06-26). Laboratory incubations also measured acetate concentrations. The 2023 laboratory incubations were also compared with laboratory incubations conducted on peat collected in 2016 (Rush et al. 2021b). This dataset contains three data files in comma-separate (.csv) format. Additional metadata are provided: three data dictionaries and a file-level metadata file in comma separate (.csv) format and a user guide in PDF (*.pdf) format.

54 ENVIRONMENTAL SCIENCES

exfor_client

A lightweight Python client and CLI for interacting with the [EXFOR Web API](https://nds.iaea.org/exfor/x4guide/API/). This tool enables searching, retrieving, and parsing experimental nuclear data — including uncertainties, covariance information, and metadata — while preserving provenance.

Grosskopf, Mike [Los Alamos National Laboratory]

2020_Experiment_2

First successful experimental run of APPL from 2020. Demonstrates a raw RGB image, metadata, and a successful mask. A Poplar tree

APPL

SPRUCE Wood Anatomy of Picea mariana and Larix laricina in SPRUCE Experimental Plots, Marcell Experimental Forest, Minnesota, July 2023

Branch samples were collected in July 2023 to measure wood anatomical traits on two dominant conifer species, Picea mariana and Larix laricina, in a bog forest at the SPRUCE (Spruce and Peatland Responses Under Changing Environments) experiment in northern Minnesota. Anatomical measurements were made on the annual rings of those branches with dates ranging from 2011-2023. Wood anatomical measurements include annual tracheid diameter, tracheid density, cell wall thickness, thickness-to-span-ratio, and conduit lumen fraction in both earlywood and latewood. Wood anatomical samples were prepared using a portable sliding microtome (G.S.L.-1 lightweight microtome, WSL) and a light microscope (Leica DM2500). This dataset spans 2011–2023, with full branch-level coverage for 2020–2023, whereas some thinner branches formed only in recent years and therefore do not contain rings from earlier years. By providing annual, treatment-specific anatomical measurements, this dataset can help quantify structural acclimation to global change, clarify links among phenology, wood formation, and hydraulic traits, and improve predictions of forest growth response under future climates. This dataset contains one data file in comma separate (.csv) format. Additional metadata are provided: one data dictionary and a file-level metadata file in comma separate (.csv) format and a user guide in PDF (*.pdf) format.

annual tracheid diameter

Best Practices for Nuclear Experiment Data Preservation at Idaho National Laboratory: A Guide for Researchers and Reactor Operators

Preserving experimental data is essential for supporting advancements in nuclear science and ensuring the longevity of Idaho National Laboratory's contributions to reactor technology and safety. This report provides a comprehensive guide to best practices for experimental data management and preservation, focusing on standardized data formats, redundancy in storage, metadata documentation, and alignment with international standards. By following these recommendations, experimentalists and reactor operators can enhance the accessibility, reproducibility, and utility of critical datasets for regulatory review, validation computational methods, and future research.

22 GENERAL STUDIES OF NUCLEAR REACTORS

SPRUCE Root Production Assessed with Manual Minirhizotrons Resolved to Plant Functional Type, 2015-2021

This dataset contains raw root length and diameter for individual roots and estimated root population production measurements from the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experimental site within the Marcell Experimental Forest in northern Minnesota, USA. Measurements started at the beginning of whole ecosystem warming manipulations in 2015 through 2021 (2015-05-26 to 2021-09-01). Root morphology and estimated production were quantified throughout the peat profile with manual minirhizotrons deployed within SPRUCE plots. Images were processed using commercial software to quantify the length and diameter of individual roots. Roots were visually assigned to a plant functional type (PFT) of either (ericaceous) shrub, herb (sedges and Maianthemum trifolium), or tree (Larix laricina, Picea mariana) based on expert opinion. The biomass of individual roots was estimated using PFT-specific allometric equations (Iversen et al., 2018). Production per day was estimated as the length of new roots produced between imaging sessions, divided by the number of days between imaging sessions. These values were placed on a m2 aboveground area basis and scaled to a standard depth of 1m (roots are not evenly distributed with depth, do not interpret value as being on a m3 basis). Maximum and average (weighted by production length) depth of each PFT were also estimated within each minirhizotron tube. Annual production was interpolated as the average of four methods to scale these data (see Weber et al, 2026). Standing crop of roots was estimated for each tube as the maximum visible amount (both length and mass) of roots of that PFT for that year. These data expand the ability of researchers to accurately estimate the belowground dynamics of peatland vegetation, as well as the role that fine roots may play in impacting the fluxes of carbon within peatlands. This dataset contains three data files in comma-separate values (*.csv) format. This dataset contains one data file in comma-separate values (.csv) format. Additional metadata are provided: three data dictionaries and a file-level metadata file in comma-separate values (.csv) format and a user guide in PDF (*.pdf) format.

54 ENVIRONMENTAL SCIENCES

SPRUCE Root Tip and Ectomycorrhizal Fungi Colonization Measurements from Ingrowth Cores, 2017

This data set contains root tip and ectomycorrhizal fungi colonization measurements taken from ingrowth cores from the SPRUCE experiment (Hanson et al. 2017) that were deployed during the 2017 growing season (2017-06 to 2017-10-01). This study explored the relationship between warming treatments and fine-root growth. Increased fine-root growth may increase root exudates and accelerate turnover, representing an underlying mechanism for peat decomposition through priming, as exudates provide a labile carbon source to the microbial community. Roots of two tree species were studied: an evergreen conifer Picea mariana (black spruce) and a deciduous conifer Larix laricina (tamarack). Measurements include root tips counts and densities by tree species and the abundance of ectomycorrhizal colonization on root tips. This dataset contains one data file in comma separate (.csv) format. Additional metadata are provided: one data dictionary and a file-level metadata file in comma separate (.csv) format and a user guide in PDF (*.pdf) format.

black spruce [Picea mariana]

SPRUCE Soil Volumetric Water Content in Experimental Plots, Marcell Experimental Forest, Minnesota, 2017-2025

This dataset contains soil volumetric water content (VWC) measurements from the Spruce and Peatland Responses Under Changing Environments (SPRUCE) Experiment. Measurements were made inside SPRUCE experimental plots in the S1 Bog at the US Forest Service Marcell Experimental Forest in northern Minnesota, USA from 2018-2025 (2018-06-14 to 2025-12-31). Observations were made with METER 10HS soil moisture sensors. To reduce bulk density related variability, 10HS sensors were placed inside mesh tubes filled with peat at a standard bulk density. The observations under standard bulk density represent relative differences in water content between hummock and hollow positions, and in response to experimental treatments. Productivity of peatlands, and their keystone species sphagnum, are highly dependent on water availability, which is affected by lateral inputs, precipitation, ground water depth and evapotranspiration. Knowledge of near surface and sphagnum water content is useful to understand peatland function. This dataset contains 8 data files in comma-separate values (.csv) format. Additional metadata are provided: one data dictionary and a file-level metadata file in comma-separate values (*.csv) format and a user guide in PDF (*.pdf) format.

Warren, Jeffrey [ORNL] (ORCID:0000000206804697)

Enabling Model Organism and Commercial Astronaut Data Access Through the NASA Open Science Data Repository

NASA’s Open Science Data Repository (OSDR) brings together omics data from NASA’s GeneLab project and non-omics data, including physiological, phenotypic, imaging, and behavioral data from NASA’s Ames Life Sciences Data Archive (ALSDA) collected from decades of space biology research, providing open and FAIR (findable, accessible, interoperable, and reusable) access of these precious data to scientists world-wide. This rich source of meticulously curated metadata and data from spaceflight and analog studies has been mined by the scientific community resulting in dozens of high impact scientific publications that reveals a complex network of molecular and physiological effects of spaceflight across living systems, from microbes to plants, to mammals. Understanding how these effects translate to the human condition is critical as we move deeper into the era of commercial space travel. However, the integration of data, specifically omics data, from astronauts is particularly challenging due to their sensitive nature. OSDR has risen to this challenge by developing a mechanism to control access to identifiable levels of omics data, such as raw sequence data, while enabling public access to processed, unidentifiable, data and associated metadata that will allow the scientific community to interrogate human astronaut data alongside data from model organisms to begin answering these critical questions. The 2021 SpaceX Inspiration4 (I4) mission collected a comprehensive atlas of biological measurements from four civilian astronauts, providing a wealth of data to characterize the effects of spaceflight on the human body. These data include both non-omics and omics assays such as direct RNA sequencing (RNA-seq), single nuclei ATAC-seq and RNA-seq, metagenomics, proteomics, and comprehensive metabolic and cytokine panels, all of which have been integrated into the OSDR system across no less than 9 studies. Each study has been carefully curated using community-backed OSDR standards for sample and assay level metadata ensuring these data are findable and accessible. In addition to hosting both raw and processed data from the principal investigator team for each assay type, the GeneLab team plans to re-process the I4 omics data using GeneLab’s standard processing pipelines. The GeneLab processed data outputs will allow for comparisons across studies on OSDR and enable visualization of these data through the OSDR data visualization platform thereby enabling data reusability and interoperability. Here we describe the robust privacy and security protocols implemented by OSDR to safeguard sensitive health data from astronauts while facilitating metadata and processed data sharing for research purposes. We further provide a road map for navigating the vast amount of data provided for each I4 study on the OSDR, including experimental design, associated experiments, payloads, and missions, data generation and analysis protocols, and associated scientific articles. Additionally, we illustrate how to interrogate the standardized metadata provided in the sample and assay tables as well as various means to download and access the data including programmatically through the GeneLab Open API (GLOpenAPI). The open access of datasets in NASA’s OSDR provides a unique opportunity for the scientific community, as well as citizen scientists and students, to continue using OSDR resources to further unlock profound insights into the consequences of space travel on the human body. Through implementation of security measures to protect sensitive human data, the OSDR seeks to strengthen the science exchange between the Biological and Physical Sciences Program and the Human Research Program, per recommendation 4-1 of the 2023-2032 Decadal Survey, and encourage further sharing and dissemination of astronaut data to provide the scientific community with the resources needed to lay the groundwork for developing targeted mitigation strategies to help withstand the rigors of long-duration spaceflight.

Amanda Marie Saravia-butler

Enabling Model Organism and Commercial Astronaut Data Access Through the NASA Open Science Data Repository

NASA’s Open Science Data Repository (OSDR) brings together omics data from NASA’s GeneLab project and non-omics data, including physiological, phenotypic, imaging, and behavioral data from NASA’s Ames Life Sciences Data Archive (ALSDA) collected from decades of space biology research, providing open and FAIR (findable, accessible, interoperable, and reusable) access of these precious data to scientists world-wide. This rich source of meticulously curated metadata and data from spaceflight and analog studies has been mined by the scientific community resulting in dozens of high impact scientific publications that reveals a complex network of molecular and physiological effects of spaceflight across living systems, from microbes to plants, to mammals. Understanding how these effects translate to the human condition is critical as we move deeper into the era of commercial space travel. However, the integration of data, specifically omics data, from astronauts is particularly challenging due to their sensitive nature. OSDR has risen to this challenge by developing a mechanism to control access to identifiable levels of omics data, such as raw sequence data, while enabling public access to processed, unidentifiable, data and associated metadata that will allow the scientific community to interrogate human astronaut data alongside data from model organisms to begin answering these critical questions. The 2021 SpaceX Inspiration4 (I4) mission collected a comprehensive atlas of biological measurements from four civilian astronauts, providing a wealth of data to characterize the effects of spaceflight on the human body. These data include both non-omics and omics assays such as direct RNA sequencing (RNA-seq), single nuclei ATAC-seq and RNA-seq, metagenomics, proteomics, and comprehensive metabolic and cytokine panels, all of which have been integrated into the OSDR system across no less than 9 studies. Each study has been carefully curated using community-backed OSDR standards for sample and assay level metadata ensuring these data are findable and accessible. In addition to hosting both raw and processed data from the principal investigator team for each assay type, the GeneLab team plans to re-process the I4 omics data using GeneLab’s standard processing pipelines. The GeneLab processed data outputs will allow for comparisons across studies on OSDR and enable visualization of these data through the OSDR data visualization platform thereby enabling data reusability and interoperability. Here we describe the robust privacy and security protocols implemented by OSDR to safeguard sensitive health data from astronauts while facilitating metadata and processed data sharing for research purposes. We further provide a road map for navigating the vast amount of data provided for each I4 study on the OSDR, including experimental design, associated experiments, payloads, and missions, data generation and analysis protocols, and associated scientific articles. Additionally, we illustrate how to interrogate the standardized metadata provided in the sample and assay tables as well as instructions for how to download and access the data. The I4 datasets described here re present the first ever comprehensive collection of commercial astronaut data.

Amanda M Saravia-Butler

Fungal mat growth and leaf colonization at the TRACE warming experiment, Mar - Aug 2024, Luquillo, Puerto Rico

This data package contains processed measurements on the growth of litter mat-forming fungi and the time to leaf colonization at the Tropical Responses to Altered Climate Experiment (TRACE). Located near the Sabana Field Research Station in Luquillo, Puerto Rico, the TRACE site is located in a mature, closed-canopy tropical rainforest within the Luquillo Experimental Forest (LEF). These data quantify fungal mat growth and the time to leaf colonization of fungi species Gymnopus johnstonii and Marasmius crinis-equi. The experiment was conducted in ambient (control) and experimentally warmed plots (4°C above ambient) during spring and summer periods to assess how litter mat-forming fungi respond to a range of environmental conditions of tropical wet forests. The data files include tables of relative fungal mat growth rates, time to leaf colonization, averages of soil temperature (°C), and number of dry days before leaf attachment. The data are stored in comma-separated values (CSV) format and viewable with any text editor, spreadsheet, or statistical software (e.g., R, Python, Excel). Associated metadata describe plot identifiers, measurement descriptions, and processing steps.

Agaric fungi

SPRUCE Bud Cold Hardiness of Trees and Shrubs in Experimental Plots, Marcell Experimental Forest, Minnesota, 2021-2025

This dataset contains bud cold hardiness measurements from the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experimental site (Hanson et al, 2017) within the Marcell Experimental Forest in northern Minnesota, USA. Cold hardiness was quantified as the temperature at which low temperature exotherms (LTEs) were identified (i.e., the temperature at which supercooled water within a bud freezes) measured by differential thermal analysis (DTA). Buds were sampled at semi-regular intervals from September 2021 through May 2025 (2021-09-25 to 2025-05-21) across four seasons (2021-2022 through 2024-2025) for four co-occurring boreal peatland tree and shrub species: Picea mariana (PIMA), Larix laricina (LALA), Rhododendron groenlandicum (RHGR), and Chamaedaphne calyculata (CHCA). Samples were collected across the experiment's warming gradient (ambient to +9 degrees Celsius (C)) and pooled by species within each enclosure. These data were used to assess the species- and season-specific effects of experimental warming on cold hardiness and cold damage risk in boreal peatland vegetation (Campos-Arguedas et al, accepted). This dataset contains one data file in comma-separate values (*.csv) format. Additional metadata are provided: a data dictionary and a file-level metadata file in comma-separate values (*.csv) format and a user guide in PDF (*.pdf) format.

Chamaedaphne calyculata

Mind the gap: Bridging the divide between AI aspirations and the reality of autonomous microscopy

What does materials science look like in the “Age of Artificial Intelligence?” Each material’s domain—synthesis, characterization, and modeling—has a different answer to this question, motivated by unique challenges and constraints. This work focuses on the tremendous potential of autonomous characterization within electron microscopy. We present our recent advancements in developing domain-aware, multimodal models for microscopy analysis capable of describing complex atomic systems. We then address the critical gap between the theoretical promise of autonomous microscopy and its current practical limitations, showcasing recent successes while highlighting the necessary developments to achieve robust, real-world autonomy.

2D materials

SPRUCE Measurements of Fine Root Production and Chemistry from Root Ingrowth Cores, Marcell Experimental Forest, Minnesota, 2022-2023

This dataset contains fine root production and tissue chemistry measurements from root ingrowth cores. Ingrowth cores were deployed in peat from June 28, 2022 to June 24, 2023 (2022-06-28 to 2023-06-24) inside SPRUCE Experiment plots at the Marcell Experimental Forest in northern Minnesota. The warming and elevated carbon dioxide (CO2) treatments in this dataset include +0 degrees Celsius (C) (+0 and +500 parts per million (ppm) elevated CO2), +4.5 degrees C (+0 and +500 ppm elevated CO2) and +9 degrees C (+0 and +500 ppm elevated CO2) for both hummocks and hollows, as well as +2.25 degrees C (+0 and +500 ppm) and +6.75 degrees C (+0 and +500 ppm) for hollows from minimum 10 cm depth from the peat surface. Measurements include root average diameter, root length, root biomass, and root tissue nitrogen (%N and δ15N) and carbon (%C and δ13C) concentration per plant functional type and microtopographical feature. Root length and biomass are standardized to 10 cm depth. These data were used to assess the warming and elevated CO2 response of fine roots across different peatland microtopographical features (hummocks and hollows) and plant functional types (shrub, spruce and larch). This dataset contains one data file in comma separate (.csv) format. Additional metadata are provided: one data dictionary and a file-level metadata file in comma separate (.csv) format and a user guide in PDF (*.pdf) format.

ESS-DIVE CSV File Formatting Guidelines Reporting