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At least 37 records · Page 2

Site-directed mutagenesis of serine 158 demonstrates its role in spinach leaf sucrose-phosphate synthase modulation

Site-directed mutagenesis of spinach sucrose-phosphate synthase (SPS) was performed to investigate the role of Ser158 in the modulation of spinach leaf SPS. Tobacco plants expressing the spinach wild-type (WT), S158A, S158T and S157F/S158E SPS transgenes were produced. Expression of transgenes appeared not to reduce expression of the tobacco host SPS. SPS activity in the WT and the S158T SPS transgenics showed light/dark modulation, whereas the S158A and S157F/S158E mutants were not similarly light/dark modulated: the S158A mutant enzyme was not inactivated in the dark, and the S157F/S158E was not activated in the light. The inability to modulate the activity of the S158A mutant enzyme by protein phosphorylation was demonstrated in vitro. The WT spinach enzyme immunopurified from dark transgenic tobacco leaves had a low initial activation state, and could be activated by PP2A and subsequently inactivated by SPS-kinase plus ATP. Rapid purification of the S158A mutant enzyme from dark leaves of transgenic plants using spinach-specific monoclonal antibodies yielded enzyme that had a high initial activation state, and pre-incubation with leaf PP2A or ATP plus SPS-kinase (the PKIII enzyme) caused little modulation of activity. The results demonstrate the regulatory significance of Ser158 as the major site responsible for dark inactivation of spinach SPS in vivo, and indicate that the significance of phosphorylation is the introduction of a negative charge at the Ser158 position.

Non-NASA Center↗

Lunar dark-mantle deposits - Possible clues to the distribution of early mare deposits

A hypothesis is outlined for developing a better understanding of the areal extent and possible composition of early but subsequently buried mare lava flows. The thesis states that in areas adjacent to maria, upland dark-mantle deposits which are older than the adjacent mare surfaces may be used as clues to the distribution and composition of earlier, now buried, mare flows which formed at the same time as the dark mantle. Conclusions as to such distributions and compositions are given.

Head, J. W.↗

The influence of a voltage ramp on the measurement of I-V characteristics of a solar cell

For efficiency and convenience the voltage applied to a Si solar cell is often fairly rapidly driven from zero to the open circuit value typically at a common rate of 1 V per millisecond. During this time the values of current are determined as a function of the instantaneous voltage thus producing an I-V characteristic. The present paper shows that the customary expressions for the current as a function of cell parameters still remain valid provided that the diffusion length in the expression for the dark current is changed from its steady state value L to the effective diffusion length L1 given by L1 = L(1 + qV/kT.tau) to the -1/2, where V is the ramp rate considered constant and tau is the lifetime of minority carriers. This result is true to a very good approximation provided that low level injection prevails.

Von Roos, O.↗

Enhanced quantum efficiency of high-purity silicon imaging detectors by ultralow temperature surface modification using Sb doping

A low temperature process for Sb doping of silicon has been developed as a backsurface treatment for high-purity n-type imaging detectors. Molecular beam epitaxy (MBE) is used to achieve very high dopant incorporation in a thin, surface-confined layer. The growth temperature is kept below 450 (deg)C for compatibility with Al-metallized devices. Imaging with MBE-modified 1kx1k charge coupled devices (CCDs) operated in full depletion has been demonstrated. Dark current is comparable to the state-of-the-art process, which requires a high temperature step. Quantum efficiency is improved, especially in the UV, for thin doped layers placed closer to the backsurface. Near 100% internal quantum efficiency has been demonstrated in the ultraviolet for a CCD with a 1.5 nm silicon cap layer.

charged coupled devices (CCDs)↗

Enhanced quantum efficiency of high-purity silicon imaging detectors by ultralow temperature surface modification using Sb doping

A low temperature process for Sb doping of silicon has been developed as a backsurface treatment for high-purity n-type imaging detectors. Molecular beam epitaxy (MBE) is used to achieve very high dopant incorporation in a thin, surface-confined layer. The growth temperature is kept below 450 °C for compatibility with Al-metallized devices. Imaging with MBE-modified 1kx1k charge coupled devices ((CCDs) operated in full depletion has been demonstrated. Dark current is comparable to the state-of-the-art process, which requires a high temperature step. Quantum efficiency is improved, especially in the UV, for thin doped layers placed closer to the backsurface. Near 100% internal quantum efficiency has been demonstrated in the ultraviolet for a CCD with a 1.5 nm silicon cap layer.

Nikzad, Shouleh↗

Molecular hydrogen in interstellar dark clouds

A simplified H2 formation mechanism is proposed in which small interstellar grains furnish the reaction sites. This mechanism results in a maximum value for the rate constant of about 2 by 10 to the -18th power per cu cm/sec for dark clouds at 10 K. Also, the nascent molecules are ejected in excited states, in qualitative agreement with Copernicus observations. A time-dependent treatment of the chemical evolution of a dark cloud with little or no ionizing radiation shows that the clouds require more than 10 million years to achieve chemical equilibrium. The observed residual atomic hydrogen in several dark clouds suggests that the clouds are 1 to 10 million years old. Other consequences of the temporal cloud model are in accord with astronomical observations.

Allen, M.↗

Quantum Limits of Space-to-Ground Optical Communications

For a pure loss channel, the ultimate capacity can be achieved with classical coherent states (i.e., ideal laser light): (1) Capacity-achieving receiver (measurement) is yet to be determined. (2) Heterodyne detection approaches the ultimate capacity at high mean photon numbers. (3) Photon-counting approaches the ultimate capacity at low mean photon numbers. A number of current technology limits drive the achievable performance of free-space communication links. Approaching fundamental limits in the bandwidth-limited regime: (1) Heterodyne detection with high-order coherent-state modulation approaches ultimate limits. SOA improvements to laser phase noise, adaptive optics systems for atmospheric transmission would help. (2) High-order intensity modulation and photon-counting can approach heterodyne detection within approximately a factor of 2. This may have advantages over coherent detection in the presence of turbulence. Approaching fundamental limits in the photon-limited regime (1) Low-duty cycle binary coherent-state modulation (OOK, PPM) approaches ultimate limits. SOA improvements to laser extinction ratio, receiver dark noise, jitter, and blocking would help. (2) In some link geometries (near field links) number-state transmission could improve over coherent-state transmission

laser communications↗

James Webb Space Telescope Studies of Dark Energy

The Hubble Space Telescope (HST) has contributed significantly to studies of dark energy. It was used to find the first evidence of deceleration at z=1.8 (Riess et al. 2001) through the serendipitous discovery of a type 1a supernova (SN1a) in the Hubble Deep Field. The discovery of deceleration at z greater than 1 was confirmation that the apparent acceleration at low redshift (Riess et al. 1998; Perlmutter et al. 1999) was due to dark energy rather than observational or astrophysical effects such as systematic errors, evolution in the SN1a population or intergalactic dust. The GOODS project and associated follow-up discovered 21 SN1a, expanding on this result (Riess et al. 2007). HST has also been used to constrain cosmological parameters and dark energy through weak lensing measurements in the COSMOS survey (Massey et al 2007; Schrabback et al 2009) and strong gravitational lensing with measured time delays (Suyu et al 2010). Constraints on dark energy are often parameterized as the equation of state, w = P/p. For the cosmological constant model, w = -1 at all times; other models predict a change with time, sometimes parameterized generally as w(a) or approximated as w(sub 0)+(1-a)w(sub a), where a = (1+z)(sup -1) is the scale factor of the universe relative to its current scale. Dark energy can be constrained through several measurements. Standard candles, such as SN1a, provide a direct measurement of the luminosity distance as a function of redshift, which can be converted to H(z), the change in the Hubble constant with redshift. An analysis of weak lensing in a galaxy field can be used to derive the angular-diameter distance from the weak-lensing equation and to measure the power spectrum of dark-matter halos, which constrains the growth of structure in the Universe. Baryonic acoustic oscillations (BAO), imprinted on the distribution of matter at recombination, provide a standard rod for measuring the cosmological geometry. Strong gravitational lensing of a time-variable source gives the angular diameter distance through measured time delays of multiple images. Finally, the growth of structure can also be constrained by measuring the mass of the largest galaxy clusters over cosmic time. HST has contributed to the study of dark energy through SN1a and gravitational lensing, as discussed above. HST has also helped to characterize galaxy clusters and the HST-measured constraints on the current Hubble constant H(sub 0) are relevant to the interpretation of dark energy measurements (Riess et al 2009a). HST has not been used to constrain BAO as the large number of galaxy redshifts required, of order 100 million, is poorly matched to HST's capabilities. As the successor to HST, the James Webb Space Telescope (JWST; Gardner et al 2006) will continue and extend HST's dark energy work in several ways.

Gardner, Jonathan P.↗

Effects of Cryogenic Temperatures on Spacecraft Internal Dielectric Discharges

Abstract Most calculations of internal dielectric charging on spacecraft use tabulated values of material surface and bulk conductivities, dielectric constants, and dielectric breakdown strengths. Many of these properties are functions of temperature, and the temperature dependences are not well known. At cryogenic temperatures, where it is well known that material conductivities decrease dramatically, it is an open question as to the timescales over which buried charge will dissipate and prevent the eventual potentially disastrous discharges of dielectrics. In this paper, measurements of dielectric charging and discharging for cable insulation materials at cryogenic temperatures (approx. 90 K) are presented using a broad spectrum electron source at the NASA Marshall Space Flight Center. The measurements were performed for the James Webb Space Telescope (JWST), which will orbit at the Earth-Sun L2 point, and parts of which will be perennially at temperatures as low as 40 K. Results of these measurements seem to show that Radiation Induced Conductivity (RIC) under cryogenic conditions at L2 will not be sufficient to allow charges to bleed off of some typical cable insulation materials even over the projected JWST lifetime of a dozen years or more. After the charging and discharging measurements are presented, comparisons are made between the material conductivities that can be inferred from the measured discharges and conductivities calculated from widely used formulae. Furthermore, the measurement-inferred conductivities are compared with extrapolations of recent measurements of materials RIC and dark conductivities performed with the charge-storage method at Utah State University. Implications of the present measurements are also given for other spacecraft that may operate at cryogenic temperatures, such as probes of the outer planets or the permanently dark cratered areas on the moon. The present results will also be of interest to those who must design or operate spacecraft in more moderate cold conditions. Finally, techniques involving shielding and/or selective use of somewhat conductive insulators are presented to prevent arc-inducing charge buildup even under cryogenic conditions.

Ferguson, Dale c.↗

Theoretical studies in interstellar cloud chemistry

This final report represents the completion of the three tasks under the purchase order no. SCPDE5620,1,2F. Chemical composition of gravitationally contracting, but otherwise quiescent, interstellar clouds and of interstellar clouds traversed by high velocity shocks, were modeled in a comprehensive manner that represents a significant progress in modeling these objects. The evolutionary chemical modeling, done under this NASA contract, represents a notable advance over the 'classical' fixed condition equilibrium models because the evolutionary models consider not only the chemical processes but also the dynamical processes by which the dark interstellar clouds may have assumed their present state. The shock calculations, being reported here, are important because they extend the limited chemical composition derivable from dynamical calculations for the total density and temperature structures behind the shock front. In order to be tractable, the dynamical calculations must severely simplify the chemistry. The present shock calculations take the shock profiles from the dynamical calculations and derive chemical composition in a comprehensive manner. The results of the present modeling study are still to be analyzed with reference to astronomical observational data and other contemporary model predictions. As far as humanly possible, this analysis will be continued with CRE's (Creative Research Enterprises's) IR&D resources, until a sponsor is found.

Chiu, Y. T.↗

Tambo Quemado: Extraordinary concentrations of REE and refractory trace elements caused by artificial heating

Buchwald examined samples of the IIIB iron Tambo Quemado (TAMQ) cut from the 130 kg main mass. He determined it had been artificially heated, at some time prior to being reported, in an attempt to obtain metal from it. Although the Widmanstatten structure appears relatively unaffected under macroscopic examination, microscopic study of etched sections reveals the effect of the heating. Taenite and plessite area boundaries are indistinct due to high temperature diffusion. Schreibersite, once present in significant amounts, has been melted. Schreibersites in the interior have resolidified in fine-grained eutectic textures surrounded by dark-etching metal rims supersaturated with phosphorus. Buchwald states that phosphate minerals were probably present originally, because graftonite, and its polymorph sarcopside (both essentially Fe3(PO4)2), are common in irons of the IIIB groups. Based on his metallographic study Buchwald estimates TAMQ was heated to 1000 C for about one hour. An interior sample from TAMQ was examined in order to determine what effect this unintended heating 'experiment' had upon the phosphate phases.

Olsen, E.↗

Some Implications of the Leptonic Annihilation of Dark Matter: Possible Galactic Radio Emission Signatures and the Excess Radio Flux of Extragalactic Origin

We give theoretical predictions for the radio emission of a dark matter candidate annihilating into 2-lepton and 4-lepton final states. We then compare our results with the known radio measurements of the sky temperature as a function of the frequency. In particular, we calculate the radio emission for some dark matter candidates annihilating into intermediate bosons that subsequently decay into a 4-lepton channel with a thermal annihilation cross-section. We show that within the range of frequencies from 20 MHz to 5 GHz, this channel can produce a stronger signature than direct annihilation into leptons.

Elaine C F S Fortes↗

Indium antimonide infrared CCD linear imaging arrays with on-chip preprocessing

A description is presented of the fabrication of a new InSb CCD chip based on an improved process which eliminates the limitations inherent with the earlier techniques. This process includes planar junction formation and an aluminum and SiO2 material system which is amenable to state-of-the-art chemical and plasma delineation techniques. Further, the new chip integrates for the first time in monolithic format InSb IR detectors with an InSb CCD. The reported experiments represent the first operation of an InSb infrared CCD array. In addition to fuller characterization of the 20-element charge-coupled infrared imaging device, several factors which influence device performance are currently being addressed. These include surface state density, the CCD output circuit, and storage time (dark current).

Thom, R. D.↗

Constraining Cosmic Microwave Background Temperature Evolution With Sunyaev–Zel’Dovich Galaxy Clusters from the Atacama Cosmology Telescope

The Sunyaev–Zel’dovich (SZ) effect introduces a specific distortion of the blackbody spectrum of the cosmic microwave background(CMB) radiation when it scatters off hot gas in clusters of galaxies. The frequency dependence of the distortion is only independent of the cluster redshift when the evolution of the CMB radiation is adiabatic. Using 370 clusters within the redshift range 0.07z1.4 from the largest SZ-selected cluster sample to date from the Atacama Cosmology Telescope, we provide new constraints on the deviation of CMB temperature evolution from the standard model a=-+0.0170.0320.029, where()()=+a-TzTz101. This result is consistent with no deviation from the standard adiabatic model. Combining it with previous, independent data sets we obtain a joint constraint ofα=−0.001±0.012. Attributing deviation from adiabaticity to the decay of dark energy, this result constrains its effective equation of state=--+w0.998eff0.0100.008.

Yunyang Li↗

Role of irregular otolith afferents in the steady-state nystagmus during off-vertical axis rotation

1. During constant velocity off-vertical axis rotations (OVAR) in the dark a compensatory ocular nystagmus is present throughout rotation despite the lack of a maintained signal from the semicircular canals. Lesion experiments and canal plugging have attributed the steady-state ocular nystagmus during OVAR to inputs from the otolith organs and have demonstrated that it depends on an intact velocity storage mechanism. 2. To test whether irregularly discharging otolith afferents play a crucial role in the generation of the steady-state eye nystagmus during OVAR, we have used anodal (inhibitory) currents bilaterally to selectively and reversibly block irregular vestibular afferent discharge. During delivery of DC anodal currents (100 microA) bilaterally to both ears, the slow phase eye velocity of the steady-state nystagmus during OVAR was reduced or completely abolished. The disruption of the steady-state nystagmus was transient and lasted only during the period of galvanic stimulation. 3. To distinguish a possible effect of ablation of the background discharge rates of irregular vestibular afferents on the velocity storage mechanism from specific contributions of the dynamic responses from irregular otolith afferents to the circuit responsible for the generation of the steady-state nystagmus, bilateral DC anodal galvanic stimulation was applied during optokinetic nystagmus (OKN) and optokinetic afternystagmus (OKAN). No change in OKN and OKAN was observed.(ABSTRACT TRUNCATED AT 250 WORDS).

NASA Discipline Neuroscience↗

LOWCAL Ground Receiver: PMT Dark Current Measurement

This paper is part of a series of papers for a research project at New Mexico State University. The project is referred to as LOWCAL or Lightweight Optical Wavelength Communications without A Laser in space. While some of the material presented is specific to tile LOWCAL project, the general procedure for measuring the dark current of a photomultiplier tube is presented.

MacCannell, John↗

Volatilisation of terpenes from Salvia mellifera

The study demonstrates significant terpene volatilisation from Salvia mellifera. Net photosynthesis and dark respiration were measured in an intact branch of a potted plant using a gas analysis system. Photosynthesis and respiration rates were determined for various temperatures. The rates were directly proportional to leaf temperature and were the same in both light and dark reactions. Using the temperature curve for the steady-state rate of terpene volatilisation and the gas exchange characteristics, the daily carbon gain and terpene loss were calculated.

Tyson, B. J.↗