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At least 37 records · Page 2

Operational Evaluation of VEGGIE Food Production System in the Habitat Demonstration Unit

The 2010 Desert Research and Technology Studies (DRATS) of the VEGGIE Food Production System in the Habitat Demonstration Unit (HDU) Pressurized Excursion Module (PEM) was the first operational evaluation of salad crop production technology in a NASA analog test. A systematic evaluation of rooting media and nutrient delivery systems were evaluated for three lettuce cultivars that have shown promise as candidates for a surface based food production system. The VEGGIE nutrient delivery system worked well, was able to be maintained by multiple operators with a minimum of training, and supported excellent lettuce growth for the duration of the test. A Hazard Analysis and Critical Control Point (HACCP) evaluation was performed using ProSan(tm) as sanitation agent prior to consumption was approved, and the crew was allowed to consume the lettuce grown using the VEGGIE light cap and gravity based nutrient delivery system at the completion of the 14-day DRAT field test. The DRAT field test validated the crew operations; Growth of all lettuce cultivars was excellent. The operational DRAT field testing in the HDU identified light quality issues related to morphology and pigment development that will need to be addressed through additional testing. Feedback from the crew, ground support personnel, and human factors leads was uniformly positive on the psychological value of having the crop production system in the excursion module. A number of areas have been identified for future work, to minimize the "footprint" of the Food Production system through creative use of unused wall and floor space in the unit.

Stutte, Gary W.

Operation Evaluation of the VEGGIE Food Production System in the Habitat Demonstration Unit

The 2010 Desert Research and Technology Studies (DRATS) of the VEGGIE Food Production System in the Habitat Demonstration Unit (HDU) Pressurized Excursion Module (PEM) was the first operational evaluation of salad crop production technology in a NASA analog test. A systematic evaluation of rooting media and nutrient delivery systems were evaluated for three lettuce cultivars that have shown promise as candidates for a surface based food production system. The VEGGIE nutrient delivery system worked well, was able to be maintained by multiple operators with a minimum of training, and supported excellent lettuce growth for the duration of the test. A Hazard Analysis and Critical Control Point (HACCP) evaluation was performed using ProSantm as sanitation agent prior to consumption was approved, and the crew was allowed to consume the lettuce grown using the VEGGIE light cap and gravity based nutrient delivery system at the completion of the 14-day DRAT field test. The DRAT field test validated the crew operations; Growth of all lettuce cultivars was excellent. The operational DRAT field testing in the HDU identified light quality issues related to morphology and pigment development that will need to be addressed through additional testing. Feedback from the crew, ground support personnel, and human factors leads was uniformly positive on the psychological value of having the crop production system in the excursion module. A number of areas have been identified for future work, to minimize the "footprint" of the Food Production system through creative use of unused wall and floor space in the unit.

Stutte, Gary W.

Identification of Fungal Colonies on Ground Control and Flight Veggie Plant Pillows

The Veggie system focuses on growing fresh produce that can be harvested and consumed by astronauts. The microbial colonies in each Veggie experiment are evaluated to determine the safety level of the produce and then differences between flight and ground samples. The identifications of the microbial species can detail risks or benefits to astronaut and plant health. Each Veggie ground or flight experiment includes six plants grown from seeds that are glued into wicks in Teflon pillows filled with clay arcillite and fertilizer. Fungal colonies were isolated from seed wicks, growth media, and lettuce (cv. 'Outredgeous') roots grown in VEG-01B pillows on ISS and in corresponding ground control pillows grown in controlled growth chambers. The colonies were sorted by morphology and identified using MicroSeq(TM) 500 16s rDNA Bacterial Identification System and BIOLOG GEN III MicroPlate(TM). Health risks for each fungal identification were then assessed using literature sources. The goal was to identify all the colonies isolated from flight and ground control VEG-01B plants, roots, and rooting medium and compare the resulting identifications.

Scotten, Jessica E.

Potential of a Plant Gas Exchange Mechanistic Model to Predict Plant Transpiration in Veggie on ISS

Plants are an essential part of long-duration space travel, as they enable food production and contribute to air revitalization through photosynthesis, and water recycling through transpiration. Understanding their growth mechanisms is essential to use them to sustain human life in space. In particular, gas exchange – e.g., CO2 absorption and water transpiration – are modified in microgravity because of the lack of buoyancy-driven convection, and in the long run, this could result in impaired plant growth. Water absorbed by the plants mainly depends on their size and on environmental conditions (air temperature, humidity and ventilation), but in microgravity watering plants is a delicate operation – too much water results in flooded roots and too little water leads in a few hours to wilted plants. This is regularly experienced in the Veggie system on ISS, which enables small-scale food production in microgravity since 2014. This presentation explores how a mechanistic model of plant gas exchange can help predict plant transpiration in Veggie and thus better predict daily watering. For each plant, inputs on canopy leaf area (acquired with daily photos), air temperature and relative humidity in the plant compartment, as well as airspeed at the top of its canopy enable accurate predictions of transpiration in microgravity. This brings a better understanding of water movement through the plant in microgravity in relation to ventilation and plant size and would result in easier management of plant watering in Veggie. Ultimately, this work could be applied to any space crop production in microgravity and be used for water management and yield predictions. This work was funded by NASA Space Biology through NASA postdoctoral program / USRA.

Lucie Poulet

Potential of a Plant Gas Exchange Mechanistic Model to Predict Plant Transpiration in Veggie on ISS

Plants are an essential part of long-duration space travel, as they enable food production and contribute to air revitalization through photosynthesis, and water recycling through transpiration. Understanding their growth mechanisms is essential to use them to sustain human life in space. In particular, gas exchange – e.g., CO2 absorption and water transpiration – are modified in microgravity because of the lack of buoyancy-driven convection, and in the long run, this could result in impaired plant growth. Water absorbed by the plants mainly depends on their size and on environmental conditions (air temperature, humidity and ventilation), but in microgravity watering plants is a delicate operation – too much water results in flooded roots and too little water leads in a few hours to wilted plants. This is regularly experienced in the Veggie system on ISS, which enables small-scale food production in microgravity since 2014. This presentation explores how a mechanistic model of plant gas exchange can help predict plant transpiration in Veggie and thus better predict daily watering. For each plant, inputs on canopy leaf area (acquired with daily photos), air temperature and relative humidity in the plant compartment, as well as airspeed at the top of its canopy enable accurate predictions of transpiration in microgravity. This brings a better understanding of water movement through the plant in microgravity in relation to ventilation and plant size and would result in easier management of plant watering in Veggie. Ultimately, this work could be applied to any space crop production in microgravity and be used for water management and yield predictions. This work was funded by NASA Space Biology through NASA postdoctoral program / USRA.

Lucie Poulet

Continued Environmental Microbiology Monitoring of The International Space Station (ISS) Veggie Unit Used for In-Flight, Crop-Based Food Systems

The International Space Station is a closed environment where rotating sets of Crewmembers live and work. This environment is monitored to ensure occupants’ health and safety during their spaceflight residency by routine Environmental Health System (EHS) collection of microbial samples including air, surface, and water. The microbial samples are collected, enumerated, and analyzed quarterly to monitor on-board system contamination and potential risks to crew health. Quarterly monitoring of the microorganisms in the ISS environment supports crew safety and contributes to a large set of microbial concentration and diversity data. The current in-inflight microbial requirements were developed using this historical data collected by the routine environmental monitoring. These in-flight microbial requirements have been established to maintain the health and safety of the spacecraft environment. This study leverages quarterly operational EHS sampling by collecting additional microbial samples from the surface of the Veggie plant production system on ISS. These samples will yield microbial concentration and diversity that can be compared and analyzed with nominal surface samples from the vehicle. The data collected in this study will aid in the development of requirements for spaceflight-based food production systems. Continued surface sampling of the internal and external surfaces of the Veggie system, along with collaboration from both Johnson Space Center (JSC) & Kennedy Space Center (KSC) scientists studying the microbiome of the veggie-crop systems, will be implemented as part of the future development of crop-based food system requirements for the ISS and beyond.

Christian Mena

Investigation of VEGGIE Root Mat

VEGGIE is a plant growth facility that utilizes the phenomenon of capillary action as its primary watering system. A cloth made of Meta Aramid fiber, known as Nomex is used to wick water up from a reservoir to the bottom of the plants roots. This root mat system is intended to be low maintenance with no moving parts and requires minimal crew interface time. Unfortunately, the water wicking rates are inconsistent throughout the plant life cycle, thus causing plants to die. Over-wicking of water occurs toward the beginning of the cycle, while under-wicking occurs toward the middle. This inconsistency of wicking has become a major issue, drastically inhibiting plant growth. The primary objective is to determine the root cause of the inconsistent wicking through experimental testing. Suspect causes for the capillary water column to break include: a vacuum effect due to a negative pressure gradient in the water reservoir, contamination of material due to minerals in water and back wash from plant fertilizer, induced air bubbles while using syringe refill method, and material limitations of Nomex's ability to absorb and retain water. Experimental testing will be conducted to systematically determine the cause of under and over-wicking. Pressure gages will be used to determine pressure drop during the course of the plant life cycle and during the water refill process. A debubbler device will be connected to a root mat in order to equalize pressure inside the reservoir. Moisture and evaporation tests will simultaneously be implemented to observe moisture content and wicking rates over the course of a plant cycle. Water retention tests will be performed using strips of Nomex to determine materials wicking rates, porosity, and absorptivity. Through these experimental tests, we will have a better understanding of material properties of Nomex, as well as determine the root cause of water column breakage. With consistent test results, a forward plan can be achieved to resolve the issue and give valuable insight for the next generation of VEGGIE.

Subbiah, Arun M.

Veggies in Space: Salad Crop Production on the ISS

NASA is currently testing Veggie, a low mass, low energy, salad crop production system on the International Space Station (ISS). Veggie grows crops with LED lights using ISS cabin air and passive watering that has presented challenges in microgravity. Initial tests included red romaine lettuce and zinnia, with testing of Chinese cabbage, and tomatoes planned. A goal is to add supplemental salad foods to the astronaut diet as we prepare for a future journey to Mars.

Massa, Gioia

Veggie on Ice: The Effects of Plant Production on Human Behavioral Health Long-Duration Antarctic Overwintering Missions

Exploration class missions pose an increased risk of adverse crew behavioral health and psychiatric conditions. Identifying and developing effective and efficient countermeasure strategies are necessary to maintain crew health and performance. Previous studies (VEG-04A and VEG-04B) in the Veggie vegetable-production system on the International Space Station have evaluated the behavioral health effects of caring for plants as a potential countermeasure for crew stresses in spaceflight. The presence of plants can augment sensory stimulation (visual, tactile, and olfactory), provide a psychological link to Earth, reduce stress, and increase happiness. However, further research is needed to understand this strategy for longer-duration missions in isolated, confined, extreme (ICE) environments. The Neumayer III Station in Antarctica is one such environment that hosts a small crew for fourteen-month missions, of which eight to nine months are the remote overwintering phase. Neumayer III also features EDEN ISS, a greenhouse facility with 12.5 m2 plant cultivation space that, like Veggie, currently supplements the crew diet with a variety of fresh produce. Up to nine crew members of the 2021 overwintering mission will complete behavioral health surveys that are designed to capture enjoyment and time spent conducting various plant-related activities, sensory stimulation, and active and passive psychological effects of having live plants and available fresh food in an ICE environment. Data will be collected once pre-mission, up to ten times in-mission at monthly increments, and once post-mission. This study aims to quantify the impact of plant care as a countermeasure strategy for human spaceflight and Antarctic exploration missions.

J M Bunchek

Plants in Space, Veggie, and Space Crop Production

As astronauts venture farther from Earth, and for longer periods, food will become increasingly critical. Crop production can supplement a packaged diet to provide additional nutrients and variety for astronauts. Testing with the Veggie chamber on the International Space Station is allowing us to understand the impacts of gravity and spaceflight on crop growth and nutritional content, and the importance of plants to astronauts living and working away from our blue home planet.

Veggie

Plants in Space, Veggie, and Space Crop Production

As astronauts venture farther from Earth, and for longer periods, food will become increasingly critical. Crop production can supplement a packaged diet to provide additional nutrients and variety for astronauts. Testing with the Veggie chamber on the International Space Station is allowing us to understand the impacts of gravity and spaceflight on crop growth and nutritional content, and the importance of plants to astronauts living and working away from our blue home planet.

Veggie

Plants in Space, Veggie, and Space Crop Production

As astronauts venture farther from Earth, and for longer periods, food will become increasingly critical. Crop production can supplement a packaged diet to provide additional nutrients and dietary variety for astronauts. Several unique challenges exist for growth of plants in microgravity and on other planetary surfaces. Testing with the Veggie chamber on the International Space Station is allowing us to understand the impacts of gravity and spaceflight on crop growth and nutritional content, and the importance of plants to astronauts living and working away from our blue home planet.

Space Crop Production

Plants in Space, Veggie, and Space Crop Production

As astronauts venture farther from Earth, and for longer periods, food will become increasingly critical. Crop production can supplement a packaged diet to provide additional nutrients and dietary variety for astronauts. Several unique challenges exist for growth of plants in microgravity and on other planetary surfaces. Testing with the Veggie chamber on the International Space Station is allowing us to understand the impacts of gravity and spaceflight on crop growth and nutritional content, and the importance of plants to astronauts living and working away from our blue home planet

Veggie

VEGGIE and the VEG-01 Hardware Validation Test

This is a presentation to NASA HQ for a lunch-and-learn detailing the Veggie testing and results. Space Life and Physical Sciences plans to record this presentation and make it available for public display.

Massa, Gioia

Veggie and the VEG-01 Hardware Validation Test

This presentation presents a brief overview of KSC plant science hardware for space and then details the Veggie hardware and the VEG-01 hardware validation test. The test results and future plans are discussed.

Massa, Gioia

Culture-Based Environmental Microbiology Monitoring of Crop-Based Space Food Systems (veggie Monitoring)

Crewmembers live and work in a closed environment that is monitored to ensure their health and safety. Quarterly monitoring of the microorganisms in the International Space Station (ISS) environment supports crew safety and contributes to a large set of microbial concentration and diversity data from air, surfaces and water samples. This study leverages quarterly operational Environmental Health System (EHS) sampling by collecting additional microbial samples from the surface of the station’s Veggie plant production system. Longer exploration missions may require spaceflight-based systems for growth of plants, and this investigation is expected to provide additional data to help establish requirements to protect these systems, plants, and crew, mitigating adverse microbial exposure.

Hamilton, Tanner

Microbiological Analysis of Mizuna Grown in the Veggie Hardware to Define Critical Control Points and Ensure the Safety of Space Grown Crops

The Veggie facility on the International Space Station has been utilized as a “pick and eat” plant growth system to provide fresh produce for crew consumption. The VEG-04 experiments completed in 2019 examined the effect of red-rich and blue-rich light treatments on the growth of mizuna as well as harvest method and resulting yield. Analysis was performed on plant tissues and associated hardware to evaluate the influence of these experimental variables on the microbial population. VEG-04A plant pillows with pre-planted Mizuna mustard seeds were launched on SpaceX-16 in December 2018. The pillows were initiated, and a single 35-day harvest was performed. Veg 04B pillows were pre-planted with Mizuna seeds and launched on SpaceX-18 in July 2019, initiated and subsequently harvested at days 29, 43 and 58. The crew consumed approximately half of the produce, and the remainder was frozen and returned for analysis. Leaves, swabs, wicking material, substrate, and roots were processed and plated on media for the enumeration and isolation of bacteria and fungi. Isolated bacterial colonies were identified using Biolog Micro ID system or MicroSEQ16S rDNA sequencing technique. Fungal colonies were identified using the MicroSEQ D2 rDNA kit. Sample extracts were plated onto specialized media to identify Escherichia coli/coliforms, Staphylococcus aureus and Salmonella sp. Results indicate that bacterial and fungal counts were higher in plants grown in red-rich lightin VEG-04A, while the opposite was true in the Veg-04B third harvest. Microbial counts increased with the repeated harvest method used in Veg-04B. These data support the understanding of environmental and horticultural practices that can affect the microbiological quality of space-grown produce grown and aid in identification of critical control points for the development of a hazard analysis critical control point plan for ISS-grown crops. This research was co-funded by the NASA’s Human Research Program and Space Biology.

Mary E Hummerick