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Vegetation transect surveys from 2022 and 2023 within the Kougarok Fire Complex (KFC) on the Seward Peninsula in Alaska, USA

This dataset contains recorded vegetation classifications from 16 transects within the Kougarok Fire Complex (KFC) area in Alaska. Each transect is 50 meters long, and vertical vegetation profiles were sampled every 50cm, resulting in 101 points recorded for each transect. At each point, a vertical rod was inserted in the ground and any living plant or substrate touching the rod was recorded from top to bottom. For each plant, the plant functional type (PFT) and species name was identified and recorded. The file "flmd.xlsx" provides file level meta data for all other included files. The file (transect_metadata.csv) contains descriptive information about each of the 16 transects (field observation year and date; latitude and longitude GPS measurements of the 0, 25, and 50 meter points; and whether that area burned during the 1971, 1997, 2002, 2015, and/or 2019 fires). The files (PFT_names.csv and species_names.csv) map the short hand labels for the plant function type (PFT) and species used in the transect surveys to their full names. The remaining files are named for each transect, and contain the results of the vegetation survey for each transect. These files contain the plant functional type and the species identified at each point. Points along the transect with more than one species at that point are listed vertically from top to bottom in these files.The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska.Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES

Fungal mat growth and leaf colonization at the TRACE warming experiment, Mar - Aug 2024, Luquillo, Puerto Rico

This data package contains processed measurements on the growth of litter mat-forming fungi and the time to leaf colonization at the Tropical Responses to Altered Climate Experiment (TRACE). Located near the Sabana Field Research Station in Luquillo, Puerto Rico, the TRACE site is located in a mature, closed-canopy tropical rainforest within the Luquillo Experimental Forest (LEF). These data quantify fungal mat growth and the time to leaf colonization of fungi species Gymnopus johnstonii and Marasmius crinis-equi. The experiment was conducted in ambient (control) and experimentally warmed plots (4°C above ambient) during spring and summer periods to assess how litter mat-forming fungi respond to a range of environmental conditions of tropical wet forests. The data files include tables of relative fungal mat growth rates, time to leaf colonization, averages of soil temperature (°C), and number of dry days before leaf attachment. The data are stored in comma-separated values (CSV) format and viewable with any text editor, spreadsheet, or statistical software (e.g., R, Python, Excel). Associated metadata describe plot identifiers, measurement descriptions, and processing steps.

Agaric fungi

Arctic shrub and Eriophorum leaf and root decomposition, northern Alaska, 2017-2018

This data package contains litter decomposition data collected from 170 plots of rapidly expanding shrub genera (Alnus, Betula, and Salix) and a widespread sedge (Eriophorum vaginatum) along a latitudinal and temperature gradient in northern Alaska. These data were produced from a litter bag experiment that took place from July 2017 to July 2018 and include mass loss and nitrogen loss decomposition metrics for both leaf and root litters. These raw data support a submitted manuscript that examines the variability in decomposition between shrub and graminoid leaf and root litters across a 1-year experiment across the graminoid-dominated Arctic tundra and reveals how deciduous shrub expansion affects litter decomposition in tundra ecosystems. Data are presented by site (n=5) and patch (shrub or sedge plot) in csv files. The site and plot location data and environmental measurement data are provided in Fraterrigo and Chen (2020). Additional methods regarding plot distribution and environmental measurements are in Chen et al. (2020) and Fraterrigo et al. (2024).

54 ENVIRONMENTAL SCIENCES

Walker Branch Watershed: Daily Stream Metabolism and Organic Carbon Spiraling Metrics in the West Fork of Walker Branch, Tennessee, USA, 2004-2010

This dataset contains daily metabolism estimates of gross primary production (GPP), ecosystem respiration (ER), and net ecosystem production (NEP), in addition to organic carbon spiraling length (SOC) and mineralization velocity (VfOC) estimates at the West Fork of Walker Branch, a small headwater stream, in the Walker Branch Watershed, Tennessee, USA. Observations were made from 2004-2010 (2004-01-01 to 2010-12-31). These data were generated to assess seasonal and interannual variability in metabolism and organic carbon spiraling and to explore potential driver variables, as analyses of intra- and interannual variability in metabolism and organic carbon spiraling are currently limited, leaving knowledge gaps in the driving mechanisms of and future changes to stream metabolism and carbon processing under climate change. Additionally, measurements of discharge (Q), stream width, stream- and canopy-level photosynthetically active radiation (PAR), water temperature, and precipitation from this time frame are included. This dataset contains one data file in comma separated (*.csv) format.

54 ENVIRONMENTAL SCIENCES

Specific conductivity and salinity of the Parker River, PIE LTER, Plum Island Sound MA, August-November 2022

This dataset contains specific conductivity and calculated salinity data of Parker River water at a tidal brackish wetland dominated by Typha angustifolia at the upper estuary of the Plum Island Sound in Newbury, Massachusetts (MA) within the Plum Island Ecosystems Long Term Ecological Research site (PIE LTER). Measurements were taken to evaluate temporal changes in surface water salinity in high frequency to characterize boundary conditions of soil and plant responses to changes in salinity. A PVC pipe was installed in a low elevation spot in the creek bank so that the bottom of the pipe sat on the sediment surface allowing flushing with water during flooding. Raw measurements were collected using an Onset HOBO U24-002 Saltwater Conductivity/Salinity data logger. The specific conductance and salinity measurements were corrected and calculated respectively using Onset’s HOBOware software and reference specific conductivity measurements taken in tandem with the first and last points recorded by the HOBO sensor. These reference measurements were taken using a HACH HQ14D Portable Conductivity Meter. Because of the installation design, only data one hour before and after high tide are used. Metadata files Typha_ctd_salinity_dd.csv and Typha_ctd_salinity_flmd.csv contain detailed information on data variables, sampling and QA/QC methods, and site location.

54 ENVIRONMENTAL SCIENCES

Redox potential in Typha-dominated tidal brackish marsh, PIE LTER, Plum Island Sound, MA, 2023

This dataset includes soil redox potential measurements collected at multiple depths within a tidal brackish wetland in the upper estuary of the Plum Island Ecosystems Long-Term Ecological Research site (PIE LTER), Plum Island Sound, Newbury, Massachusetts (MA). Measurements were taken to evaluate temporal variation in redox potential in relation to hydrological events at three replicate locations. Data were recorded every 5 minutes using a Campbell Scientific Volt116 connected to a CR6 datalogger with SWAP instrument redox probes (ORP-30-4-B) and reference electrodes. Measurements were made at the AmeriFlux site US-PLo at four soil depths (5, 10, 15, and 30 cm). The file redox_soiltemp_2023.csv contains temperature-corrected redox values and soil temperature following Silva-Machado et al. (2024). Metadata files redox_soiltemp_dd.csv and redox_soiltemp_2023_flmd.csv provide detailed descriptions of variables and site locations.

54 ENVIRONMENTAL SCIENCES

Upland tidal brackish marsh specific conductivity and salinity measurements, PIE LTER, Plum Island Sound, MA, 2023

This dataset includes raw and corrected specific conductivity, temperature, and calculated salinity measurements collected at 10 cm depth in a Typha angustifolia-dominated tidal brackish wetland at the upper estuary of Plum Island Sound in Newbury, Massachusetts (MA) within the Plum Island Ecosystems Long Term Ecological Research (PIE LTER) site. Measurements were taken to evaluate temporal variation in porewater salinity (a proxy for porewater sulfate concentration) in high frequency to assess soil and plant responses to changes in salinity. Measurements were collected using an Onset HOBO U24-002 Saltwater Conductivity/Salinity data logger deployed in a well. Specific conductivity was corrected using non-linear temperature compensation, and salinity was calculated using the Practical Salinity Scale 1978 via Onset's HOBOware software. Reference conductivity measurements to correct for sensor drift were taken at the start and end of each deployment using a HACH HQ14D Portable Conductivity Meter. Data were then filtered in MATLAB to remove values logged while the sensor was out of the well or during post-deployment equilibration. Detailed metadata, including variable descriptions, sampling methods, QA/QC procedures, and site information, are provided in the files: Typha_ctd_salinity_dd.csv and Typha_MI_ctd_salinity_2023_flmd.csv.

54 ENVIRONMENTAL SCIENCES

Upland tidal brackish marsh specific conductivity and salinity measurements, PIE LTER, Plum Island Sound, MA, May-December 2022

This dataset includes raw and corrected specific conductivity, temperature, and calculated salinity measurements collected at 10 cm depth in a Typha angustifolia-dominated tidal brackish wetland at the upper estuary of Plum Island Sound in Newbury, Massachusetts (MA) within the Plum Island Ecosystems Long Term Ecological Research (PIE LTER) site. Measurements were taken to evaluate temporal variation in porewater salinity (a proxy for porewater sulfate concentration) in high frequency to assess soil and plant responses to changes in salinity. Measurements were collected using an Onset HOBO U24-002 Saltwater Conductivity/Salinity data logger deployed in a well. Specific conductivity was corrected using non-linear temperature compensation, and salinity was calculated using the Practical Salinity Scale 1978 via Onset's HOBOware software. Reference conductivity measurements to correct for sensor drift were taken at the start and end of each deployment using a HACH HQ14D Portable Conductivity Meter. Data were then filtered in MATLAB to remove values logged while the sensor was out of the well or during post-deployment equilibration. Detailed metadata, including variable descriptions, sampling methods, QA/QC procedures, and site information, are provided in the files: Typha_ctd_salinity_dd.csv and Typha_MI_ctd_salinity_2023_flmd.csv.

54 ENVIRONMENTAL SCIENCES

Specific conductivity and salinity of the Parker River, PIE LTER, Plum Island Sound MA, March-November 2023

This dataset contains specific conductivity and calculated salinity data of Parker River water at a tidal brackish wetland dominated by Typha angustifolia at the upper estuary of the Plum Island Sound in Newbury, Massachusetts (MA) within the Plum Island Ecosystems Long Term Ecological Research site (PIE LTER). Measurements were taken to evaluate temporal changes in surface water salinity in high frequency to characterize boundary conditions of soil and plant responses to changes in salinity. A PVC pipe was installed in a low elevation spot in the creek bank so that the bottom of the pipe sat on the sediment surface allowing flushing with water during flooding. Raw measurements were collected using an Onset HOBO U24-002 Saltwater Conductivity/Salinity data logger. The specific conductance and salinity measurements were corrected and calculated respectively using Onset’s HOBOware software and reference specific conductivity measurements taken in tandem with the first and last points recorded by the HOBO sensor. These reference measurements were taken using a HACH HQ14D Portable Conductivity Meter. Because of the installation design, only data one hour before and after high tide are used. Metadata files Typha_ctd_salinity_dd.csv and Typha_ctd_salinity_flmd.csv contain detailed information on data variables, sampling and QA/QC methods, and site location.

54 ENVIRONMENTAL SCIENCES

WHONDRS River Corridor Sediment and Water Geochemistry and In Situ Sensor Data from 7 Perennial and 7 Intermittent Streams across San Antonio, Texas (v3)

This dataset supports a broader study examining the effects of intermittency on sediment respiration. The dataset provides sediment and surface water geochemistry and in situ sensor data from 7 perennial and 7 intermittent streams in San Antonio, Texas. Each stream/site was visited both in summer during base flow (July-September 2023) and winter during peak flow (January-February 2024). Related data were collected and will be published separately in collaboration with A. Veach. The data package was originally published in April 2025. It was updated in June 2025 (v2; modified and new files) and September 2025 (v3; modified files). See the change history section in the readme for more details. For details on how to navigate data packages generated by this project, see https://data.ess-dive.lbl.gov/portals/PNNLRiverCorridorSFA/About. This dataset is comprised of two folders of field photos and videos, one folder of raw Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) data and one main data folder containing (1) file-level metadata; (2) data dictionary; (3) field metadata; (4) readme; (5) international generic sample number (IGSN) mapping file; (6) field protocol; (7) a subfolder with sample data; and (8) a subfolder with sensor data. The sample data subfolder contains (1) surface water and sediment dissolved organic carbon (DOC, measured as non-purgeable organic carbon, NPOC) data and averages; (2) surface water and sediment total nitrogen data and averages; (3) sediment grain size data; (4) sediment iron (II) data and averages; (5) wet sediment mass, dry sediment mass, water mass, and wet sediment volume in incubation and sediment ICR vials; (7) sediment incubation respiration rate data and averages; (8) normalized respiration rate data and averages; (9) methods codes; (10) sediment percent carbon and nitrogen; (11) sediment X-ray diffraction (XRD) data; (12) gravimetric moisture and averages; (13) a subfolder with sediment incubation respiration data, scripts, and plots; (14) surface water and sediment FTICR methods; and (15) a subfolder of 9.4 Tesla (9.4T) FTICR-MS data. This folder contains five subfolders, one containing the sediment .xml data files, one containing the water .xml files, one containing the sediment CoreMS output files, one containing the water CoreMS output files, and the other containing instructions and scripts for processing the files in CoreMS (https://github.com/EMSL-Computing/CoreMS). The sensor data subfolder contains (1) a subfolder with miniDOT dissolved oxygen and temperature data and plots; (2) miniDOT dissolved oxygen and temperature summary data; and (3) miniDOT installation methods. All files are .csv, .pdf, .R, .xml, .d, .html, .Rmd, .py, .cal, .json, .jpg, .jpeg, .png, .mov, or .mp4. CORRECTION: The data processing methods for FTICR described in “v3_WHONDRS_AV1_Methods_Codes.csv” mistakenly indicate that users should process the data in Formultitude. The corrected description should read: “Both unprocessed and processed data are provided to allow users flexibility in data processing. Instructions and scripts for processing the data using CoreMS are included.” CORRECTION: Carbon and nitrogen content are reported as percentages. The current column headers "01395_C_percent_per_mg" and "01397_N_percent_per_mg" are incorrect. These should read "01395_C_percent" and "01397_N_percent" and will be corrected in the next version of this data package.

54 ENVIRONMENTAL SCIENCES

Hydrologic Model Data for the East Fork Poplar Creek Watershed Simulated with the Advanced Terrestrial Simulator (ATS): Streamflow and Network Expansion–Contraction Dynamics

This dataset supports hydrologic modeling and stream network expansion–contraction analysis for the East Fork Poplar Creek (EFPC) Watershed in Tennessee. It includes a Jupyter notebook for model setup, model configuration files, simulation outputs, and derived products used to evaluate model performance and investigate stream dynamics under varying hydrologic conditions. The dataset was generated using the Watershed Workflow Python package and the Advanced Terrestrial Simulator (ATS), enabling integrated surface–subsurface hydrologic simulations using a stream-aligned mesh. Outputs include high-resolution time series of streamflow, active network length, water table depth, and related hydrologic variables. Also included are spatially explicit stream persistency indices and classifications of reaches as perennial or non-perennial. These data facilitate reproducibility and support further research on stream intermittency and variability in network extent.The model data archive is organized in following directories:1) model_setup_inputsContains the Watershed Workflow Jupyter notebooks (accessed through any open source code editor), selected input datasets, and resulting ATS input files, including XML files (access through any open source code editor), computational mesh (.exo files can be viewed using Paraview), and meteorological forcing files (.h5 files can be accessed through h5py python package and HDFView open source software). 2) model_outputsIncludes ATS simulation outputs relevant to this study. Time series of spatially integrated or averaged variables (e.g., streamflow, water table depth) are provided as CSV files. Select spatial fields (e.g., ponded depth and water table depth) are saved as pickled Python objects to reduce file size, and can be accessed through pickle package in Python. Key geometry objects from Watershed Workflow—such as the surface mesh and river tree—are also included to support analysis of streamflow persistency and expansion–contraction dynamics. These files can also be accessed through Watershed Workflow Python package.3) model_evaluationProvides observed streamflow time series and field survey-based flow regime classifications used to evaluate model performance. Jupyter notebooks for processing ATS outputs and comparing model predictions with observations to build confidence in the model prior to scientific analysis are also included.4) Q_L_relationshipsContains workflows for generating time series of discharge, active network length, and related hydrologic variables used in the stream network expansion–contraction analysis. Includes routines for delineating baseflow-dominated periods. For each catchment, notebooks and processed data (as pickled DataFrames accessed through Pandas Python package) are provided. 5) figure_scriptsProvides the Jupyter notebooks used to generate the figures presented in the paper.

54 ENVIRONMENTAL SCIENCES

Chamber flux measurements at US-ORv located in Columbus, OH from 2010 to 2014

Closed and flow through chamber methane and carbon dioxide flux measurements were taken at different water depths, vegetation cover types, and times of day at the Wilma H. Schiermeier Olentangy River Wetland Research Park (ORWRP) from 2010 to 2014 to investigate the effects of these variables on gas exchange. The ORWRP consists of two urban experimental wetland basins constructed in 1994 within the footprint of the AmeriFlux tower US-ORv that was functional from 2011 to 2016. Chamber measurements are separated into flow through and closed chamber files (flow_through.csv, closed_chamber.csv) with location labels corresponding with coordinates listed in the metadata file (Location_ID.csv). All files are .csv and could be viewed or analyzed with Excel, MATLAB, Python, or R.

EARTH SCIENCE > ATMOSPHERE > ATMOSPHERIC CHEMISTRY

Synoptic NO3 in Slate River Watershed, Colorado (2022)

The synoptic nitrate (NO3) dataset in the Slate River Watershed, Colorado consists of NO3 data collected at 19 locations three times during the summer of 2022. Stream samples were collected in early summer (early July), mid summer (late August), and late summer (late September). The samples include mainstem, tributary, and point source input water samples. These data were collected to evaluate spatiotemporal variability in stream NO3 during the summer, and evaluate anthropogenic controls on stream NO3 dynamics. This data package contains: (1) a csv of all NO3 samples and (2) a csv of locations for each sampling site. The dataset additionally includes a file-level metadata (flmd.csv) file that lists each file contained in the dataset with associated metadata; and a data dictionary (dd.csv) file that contains column/row headers used throughout the files along with a definition, units, and data type. This work was supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231.

EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > FRESHWATE

Porewater chemistry in Typha-dominated brackish tidal marsh, PIE LTER, Plum Island Sound, MA, July 2022–September 2024

This dataset contains profile measurements of porewater constituents taken on 3-4 days across the growing seasons in 2022, 2023, and 2024 in a tidal brackish marsh within the Plum Island Ecosystems Long Term Ecological Research site (PIE LTER), located in the Plum Island Sound, Massachusetts (MA). Measurements were taken to monitor changes in porewater chemistry induced by seasonal saltwater intrusion at the site. Samples were taken in two locations: one was close to the creek bank and the other in the marsh interior. Water was sampled from 2-5 depths between the surface to 50cm using a sipper consisting of a hollow stainless steel rod with an opening at the end similar to that described in (Berg & McGlathery, 2001). The rod was pushed into the sediment to the desired depth, typically every 10cm, and water samples were taken by syringe. Water was not obtained at all depths. Samples were preserved and analyzed in the lab. Metadata files Typha_porewater_sipper_dd.csv and Typha_porewater_sipper_flmd.csv contain detailed information on data variables, sampling and QA/QC methods, and site location.

54 ENVIRONMENTAL SCIENCES

Redox potential in Typha-dominated tidal brackish marsh, PIE LTER, Plum Island Sound, MA, June–December 2022

This dataset includes soil redox potential measurements collected at multiple depths within a tidal brackish wetland in the upper estuary of the Plum Island Ecosystems Long-Term Ecological Research site (PIE LTER), Plum Island Sound, Newbury, Massachusetts. Measurements were taken to evaluate temporal variation in redox potential in relation to hydrological events at three replicate locations. Data were recorded every 5 minutes using a Campbell Scientific Volt116 connected to a CR6 datalogger with SWAP instrument redox probes (ORP-30-4-B) and reference electrodes. Measurements were made at the AmeriFlux site US-PLo at four soil depths (5, 10, 15, and 30 cm). The file redox_soiltemp_2022.csv contains temperature-corrected redox values and soil temperature following Silva-Machado et al. (2024). Metadata files redox_soiltemp_dd.csv and redox_soiltemp_flmd.csv provide detailed descriptions of variables and site locations.

54 ENVIRONMENTAL SCIENCES

Subsets of geostationary satellite data over international observing network sites for studying the diurnal dynamics of energy, carbon, and water cycles

The latest generation of geostationary satellites provide Earth observations similar to widely used polar-orbiting sensors but at intervals as frequently as every 5–10 min, making them ideal for studying the diurnal dynamics of land–atmosphere interactions. The NASA Earth Exchange (NEX) group created the GeoNEX datasets by collating data from several geostationary platforms, including GOES-16/17/18, Himawari-8/9, and GK-2A, and placing them on a common grid to facilitate use by the Earth science community. Here, we document the GeoNEX Coincident Ground Observations (GeCGO) dataset for terrestrial ecosystem studies and provide examples for its use. Currently, GeCGO provides GOES-16 Advanced Baseline Imager (ABI) data over a 10 km × 10 km area surrounding 1586 network sites across the Americas. GeCGO makes it easy to compare the time series of geostationary data with the diurnal ground observations, including carbon/water fluxes and aerosol optical depth, and is extensible to other regions. We also develop GeoNEXTools to facilitate analyses that require both GeoNEX data and other NASA satellite data. The objectives of this paper are to introduce GeCGO and GeoNEXTools and demonstrate their applications. First, we describe the details of GeCGO and GeoNEXTools. Second, we explain how GeCGO can be integrated with other satellite data. Finally, we showcase comparisons between GeCGO and observations from three ground-based networks. GeCGO is available at https://doi.org/10.25966/y5pe-xp41 (Hashimoto et al., 2025).

Hashimoto, Hirofumi [NASA Ames Research Center (AR

Patch-level CO2 and CH4 fluxes and porewater concentrations in experimental wetlands, 5 and 10 PPT saltwater intrusion simulations, Louisiana 2023-2024

This dataset containes carbon dioxide (CO2) and methane (CH4) flux measurements collected from wetland vegetation patches dominated by Typha domingensis and Panicum hemitomon to assess greenhouse gas flux responses to experimental saltwater intrusion (SWI) pulses. Measurements were conducted before, during, and after simulated SWI events at target salinities of approximately 5 parts per thousand (ppt) with durations of 6, 10, and 17 days and 10 ppt with a duration of 48 days, alongside a control wetland (with no salinity added, flood manipulation only). These data were generated to evaluate how the magnitude and duration of SWI alter wetland carbon exchange and related biogeochemical and plant responses. This data package includes flux measurements from the wetland surface (i.e, soil/water surface and enclosed vegetation) and from the soil/water surface only; porewater and surface water concentrations of CO2 and CH4; salinity, pH, electrical conductivity collected in porewater (at 5, 10, and 20 cm soil depths) and in surface water; soil redox potential; leaf spectral indices, leaf vapor pressure deficit, stomatal conductance; water level, salinity, and photosynthetically active radiation; and aboveground biomass.

EARTH SCIENCE > AGRICULTURE > SOILS > SOIL RESPIRA

Old Woman Creek Wetland Sediment and Electrochemical Sensor Microbial Community, 2023

We are developing a technique to monitor microbiological activities referred to as zero resistance ammetry, which entails the deployment of graphite electrodes in sediments. Measurement of current between electrodes of contrasting redox regimes and/or predominant terminal electron accepting processes can be used as an indicator of the extents of microbiological activity. We deployed an electrode array at depths of 2 mm, 4 mm, 76 mm, 78 mm, 152 mm, 154 mm, 227 mm, and 229 mm below the wetland sediment water interface in the Old Woman Creek National Estuarine Research Center, Huron, OH, USA (Lat. = 41.380833, Long. = -82.508889). A core was collected from adjacent sediment and subsamples were collected from depth intervals of 0 – 25 mm, 25 – 127 mm, 127 – 128 mm, and below 178 mm. To determine if the microbial communities attached to the electrodes were reflective of the adjacent sediment-associated microbial community, we conducted a 16S rRNA gene-based (V4 region) survey of these respective materials. This data package contains the results of these surveys, including metadata on the depths from which samples were collected (samples.csv), DNA extraction and sequencing information (OWC_DEPTH_AMPLICON_SEQUENCING_METADATA), sequence processing information (OWC_DEPTH_BIOINFORMATIC_METADATA.csv), an operational taxonomic unit (OTU) table (OWC_DEPTH_97OTUS_TABLE.csv), and nucleotide sequences of OTUs (OWC_DEPTH_97OTUS_SEQS.fasta). All files can be opened using a text-editing application. The fasta file is compatible with bioinformatics applications.

54 ENVIRONMENTAL SCIENCES