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NASA's Planetary Protection Program to Assure Mission Safety and Success

NASA's planetary protection program seeks to understand and control harmful contamination of solar systems targets of exploration by terrestrial contamination and prevent harmful biological contamination of the Earth-Moon system by extraterrestrial life, should it exist. To accomplish these objective's NASA has developed a balanced safety and mission assurance strategy that leverages COSPAR Policy guidelines, workshops, scientific consensus, partnerships and international working groups to develop policy and implementation guidelines. Upcoming crewed missions to the Moon and Mars, as well as robotic missions to small solar system bodies, Europa, Titan and Mars are some of the driving activities of astrobiological interest that continue to emphasize the importance of planetary protection throughout the project life cycle.Development of a responsive and updated agency planetary protection policy has been a focus area in supporting upcoming mission opportunities for exploration to include Mars sample return and crewed mission concepts. An extensive update of this policy is underway which encompasses crewed and robotic procedural polices, a general technical requirements standard, and an implementation handbook. During this timeframe NASA has been working with the international community to develop scientific consensus, and to identify and fill in knowledge gaps for developing balanced policy guidelines, incorporation of risk informed decision making and quantitative technical standards. NASA has developed a planetary protection roadmap as a technology management strategy to track and monitor the development of each knowledge gaps. The Committee of Space Research (COSPAR) Policy on Planetary Protection and the National Academies of Science, Engineering, and Medicine's Committee of Planetary Protection are used to inform updates to the planetary protection polices and guidelines. This integrated strategy for planetary protection seeks to provide a transparent, structured approach for enabling missions, providing guidance for NASA and NASA partnered missions, and being responsive to the increased interest and activities in space exploration whilst maintaining an understanding and control of harmful contamination.

Planetary Protection

Planetary Protection, Parts, and Perception: The Ranger Missions as the Origin Story of the Myth of Planetary Protection-Related Mission Failures

There has been a long-standing perspective in the planetary engineering community that the use of planetary protection sterilization techniques such as dry heat microbial reduction (DHMR) have led to significant mission failures in the past. This perception has led to a hesitation to craft mission designs that incorporate sterilization beyond the component-level. Via interviews with long-standing members of the planetary engineering community across the Agency, we have identified the root historical missions that have driven this perspective. The source, or Origin Story, which has been passed down to subsequent generations of engineers, is the failures tied to the early series of the Ranger missions. In this presentation, we examine the details behind the Ranger failures, the majority of which are non-planetary protection sterilization-related, and bring to light the myth of this perception.

planetary protection

NASA’s Planetary Protection Program to Assure Mission Safety and Success

NASA’s planetary protection program seeks to understand and control harmful contamination of solar systems targets of exploration by terrestrial contamination and prevent harmful biological contamination of the Earth-Moon system by extraterrestrial life, should it exist. To accomplish these objective’s NASA has developed a balanced safety and mission assurance strategy that leverages COSPAR Policy guidelines, workshops, scientific consensus, partnerships and international working groups to develop policy and implementation guidelines. Upcoming crewed missions to the Moon and Mars, as well as robotic missions to small solar system bodies, Europa, Titan and Mars are some of the driving activities of astrobiological interest that continue to emphasize the importance of planetary protection throughout the project life cycle. Development of a responsive and updated agency planetary protection policy has been a focus area in supporting upcoming mission opportunities for exploration to include Mars sample return and crewed mission concepts. An extensive update of this policy is underway which encompasses crewed and robotic procedural polices, a general technical requirements standard, and an implementation handbook. During this timeframe NASA has been working with the international community to develop scientific consensus, and to identify and fill in knowledge gaps for developing balanced policy guidelines, incorporation of risk informed decision making and quantitative technical standards. NASA has developed a planetary protection roadmap as a technology management strategy to track and monitor the development of each knowledge gaps. The Committee of Space Research (COSPAR) Policy on Planetary Protection and the National Academies of Science, Engineering, and Medicine’s Committee of Planetary Protection are used to inform updates to the planetary protection polices and guidelines. This integrated strategy for planetary protection seeks to provide a transparent, structured approach for enabling missions, providing guidance for NASA and NASA partnered missions, and being responsive to the increased interest and activities in space exploration whilst maintaining an understanding and control of harmful contamination.

James Benardini

Planetary protection implementation on future Mars lander missions

A workshop was convened to discuss the subject of planetary protection implementation for Mars lander missions. It was sponsored and organized by the Exobiology Implementation Team of the U.S./Russian Joint Working Group on Space Biomedical and Life Support Systems. The objective of the workshop was to discuss planetary protection issues for the Russian Mars '94 mission, which is currently under development, as well as for additional future Mars lander missions including the planned Mars '96 and U.S. MESUR Pathfinder and Network missions. A series of invited presentations was made to ensure that workshop participants had access to information relevant to the planned discussions. The topics summarized in this report include exobiology science objectives for Mars exploration, current international policy on planetary protection, planetary protection requirements developed for earlier missions, mission plans and designs for future U.S. and Russian Mars landers, biological contamination of spacecraft components, and techniques for spacecraft bioload reduction. In addition, the recent recommendations of the U.S. Space Studies Board (SSB) on this subject were also summarized. Much of the discussion focused on the recommendations of the SSB. The SSB proposed relaxing the planetary protection requirements for those Mars lander missions that do not contain life detection experiments, but maintaining Viking-like requirements for those missions that do contain life detection experiments. The SSB recommendations were found to be acceptable as a guide for future missions, although many questions and concerns about interpretation were raised and are summarized. Significant among the concerns was the need for more quantitative guidelines to prevent misinterpretation by project offices and better access to and use of the Viking data base of bioassays to specify microbial burden targets. Among the questions raised were how will the SSB recommendations be integrated with existing Committee on Space Research (COSPAR) policy and how will they apply to and affect Mars '94, Mars '96, MESUR Pathfinder, and MESUR Network missions? One additional topic briefly considered at the workshop was the identification of some issues related to planetary protection considerations for Mars sample return missions. These issues will form the basis for a follow-on joint U.S./Russian workshop on that subject.

Howell, Robert

Advanced Planetary Protection Technologies for the Proposed Future Mission Set

Planetary protection is the discipline of protecting solar system objects from harmful contamination resulting from the activities of interplanetary spacecraft, and of similarly protecting the Earth from uncontrolled release of a putative extra-terrestrial organism from returned extra-terrestrial samples. Planetary protection requirements for Mars are becoming further refined as more is understood about the nature of the Martian environment as a potential habitat. Likewise, increased understanding of the limits of life on Earth is informing planetary protection policy. This presentation will discuss recent technology developments, ongoing work and future challenges of implementing planetary protection for the proposed future mission set.

Europa

Life Support and Habitation and Planetary Protection Workshop

A workshop entitled "Life Support and Habitation and Planetary Protection Workshop" was held in Houston, Texas on April 27-29, 2005 to facilitate the development of planetary protection guidelines for future human Mars exploration missions and to identify the potential effects of these guidelines on the design and selection of related human life support, extravehicular activity and monitoring and control systems. This report provides a summary of the workshop organization, starting assumptions, working group results and recommendations. Specific result topics include the identification of research and technology development gaps, potential forward and back contaminants and pathways, mitigation alternatives, and planetary protection requirements definition needs. Participants concluded that planetary protection and science-based requirements potentially affect system design, technology trade options, development costs and mission architecture. Therefore early and regular coordination between the planetary protection, scientific, planning, engineering, operations and medical communities is needed to develop workable and effective designs for human exploration of Mars.

Hogan, John A.

Bayesian Framework for Bioburden Density Estimation in Planetary Protection

To comply with the international planetary protection policy set forth by the Committee on Space Research and NASA Agency level requirements, spacecraft destined to biologically sensitive planetary bodies have to minimize terrestrial biological contamination. Analysis, testing and inspection are the standard forward verification activities that are used to demonstrate compliance with the biological contamination requirements. For testing of spacecraft surface areas, a swab or wipe sample is collected from surfaces prior to last access and subsequently processed in the lab using NASA Approved Planetary Protection Methods for Culture Based Assays. Raw data resulting from this assay is then statistically treated employing a mathematical paradigm stemming from the 1970’s Viking Lander Project to generate the bioburden density and total microbial bioburden present. This standard approach arbitrarily accounts for error and provides an upper conservative bound as it reports the maximum number of spores estimated to be present on flight hardware surfaces. A bioburden density estimate factors in the following variables: the observed bioburden count, representative volume processed, sampling efficiencies. Notably, to account for error in the approach, a 0 observed count is arbitrarily changed to a count of 1 for each hardware grouping. The data generated by spacecraft bioburden verification campaigns in the past have resulted in <80% of wipes and <90% of swabs containing a bioburden count of 0. As such, having a robust and well documented statistical approach for dealing with the probability of low incident rates is necessary to be able to estimate spacecraft bioburden. Being able to statistically describe the bioburden distribution and associated confidence level is a gamechanger for the development of bioburden allocations during mission design and will allow for tighter management of risk throughout spacecraft build. Thus, Empirical Bayes statistical approach was evaluated to estimate the microbial bioburden on spacecraft to mitigate the aforementioned mathematical concerns and provide a probabilistic bioburden distribution of the flight hardware surface. For application of this approach to performing bioburden calculations, a range of non-informative prior assumptions on hardware surfaces are explored for Bayesian analyses while informative priors using posterior distributions from prior assays are utilized for Empirical Bayes analyses. Several non-informative priors are currently under investigation to assess fitness including use of these priors to serve as a foundation to build off of NASA specification values or a basis of risk to account for unknowns during the integration and testing process. Informative priors under consideration are generated using sampled bioburden values from hardware originating within like processing environments (e.g. vendor cleaning process or similar assembly process), temporal spacecraft status events as a prediction for hardware cleanliness of future samples, and heritage system bioburden actuals to predict allocation for subsequent missions. Informative priors and probabilistic bioburden distributions are then validated using data sets from the Mars Exploration Rover, Mars Science Laboratory, and InSight missions. Using Empirical Bayes approach to generate a probabilistic bioburden distribution as demonstrated through mission use cases provides a valid approach for use in the end-to-end requirements verification process.

97 - MATHEMATICS AND COMPUTING

Mars Technology Program Planetary Protection Technology Development

The objectives of the NASA Planetary Protection program are to preserve biological and organic conditions of solar-system bodies for future scientific exploration and to protect the Earth from potential hazardous extraterrestrial contamination. As the exploration of solar system continues, NASA remains committed to the implementation of planetary protection policy and regulations. To fulfill this commitment, the Mars Technology Program (MTP) has invested in a portfolio of tasks for developing necessary technologies to meet planetary protection requirements for the next decade missions.

Mars technology program

NASA Planetary Protection Handbook

This handbook provides guidance to mission providers and Planetary Protection (PP) practitioners on implementing PP measures for both robotic and crewed space missions. This handbook represents major updates to PP practices since a previously drafted handbook from 2010. In 2017, NASA reorganized the Office of Planetary Protection (OPP) from the Science Mission Directorate (SMD) to a Technical Authority (TA) within the Office of Safety and Mission Assurance (OSMA). With this organizational change came a complete overhaul of NASA’s PP policy and technical standards. This handbook reflects the latest NASA PP policy updates in NPR 8715.24, Planetary Protection Provisions for Robotic Extraterrestrial Missions and technical requirements of NASA-STD-8719.27, Implementing Planetary Protection Requirements for Space Flight. This handbook is a companion document to the NPR and technical standard and provides guidance, best practices, background information, and advice for practitioners to consider when implementing PP on NASA missions and NASA-partnered missions.

Nick Benardini

Post Viking planetary protection requirements study

Past planetary quarantine requirements were reviewed in the light of present Viking data to determine the steps necessary to prevent contamination of the Martian surface on future missions. The currently used term planetary protection reflects a broader scope of understanding of the problems involved. Various methods of preventing contamination are discussed in relation to proposed projects, specifically the 1984 Rover Mission.

Wolfson, R. P.

Design Tools for Cost-Effective Implementation of Planetary Protection Requirements

Since the Viking missions to Mars in the 1970s, accounting for the costs associated with planetary protection implementation has not been done systematically during early project formulation phases, leading to unanticipated costs during subsequent implementation phases of flight projects. The simultaneous development of more stringent planetary protection requirements, resulting from new knowledge about the limits of life on Earth, together with current plans to conduct life-detection experiments on a number of different solar system target bodies motivates a systematic approach to integrating planetary protection requirements and mission design. A current development effort at NASA's Jet Propulsion Laboratory is aimed at integrating planetary protection requirements more fully into the early phases of mission architecture formulation and at developing tools to more rigorously predict associated cost and schedule impacts of architecture options chosen to meet planetary protection requirements.

mission architecture

Mars Technology Program: Planetary Protection Technology Development

This slide presentation reviews the development of Planetary Protection Technology in the Mars Technology Program. The goal of the program is to develop technologies that will enable NASA to build, launch, and operate a mission that has subsystems with different Planetary Protection (PP) classifications, specifically for operating a Category IVb-equivalent subsystem from a Category IVa platform. The IVa category of planetary protection requires bioburden reduction (i.e., no sterilization is required) The IVb category in addition to IVa requirements: (i.e., terminal sterilization of spacecraft is required). The differences between the categories are further reviewed.

Mars Technology Program

Planetary Protection Lunar Policy: A Case Study in Balancing COSPAR Guidelines, Scientific Consensus, NASA Policy, and Mission Implementation

With the increase of missions to the Earth’s Moon over the next decade, NASA initiated an assessment and review of the policy and protection of Earth’s Moon to enable scientific exploration. The assessment involved gathering scientific consensus regarding the Earth’s Moon and proposed mission operations considering an understanding of the desired science needs for the Moon. This process involved seeking advice from the National Academies of Science, Engineering, and Medicine’s (NASEM) Committee on Planetary Protection, engagement with the Committee on Space Research (COSPAR) Planetary Protection Panel (PPP), and consultation within NASA. To begin this process, NASA issued a NASA Interim Directive (NID) 8715.128 entitled, “Planetary Protection Categorization for Robotic and Crewed Missions to the Earth’s Moon” in July of 2019. This NID defined sensitive regions (e.g., permanently shadowed regions) on the Moon and required reporting on missions to these sensitive areas. Meanwhile, a NASEM study on the impact of human activities on lunar polar volatiles and the scientific value of protecting the surface and subsurface regions of the Earth’s Moon from organic and biological contamination was initiated. This resulted in a NASEM report entitled, “Planetary Protection for the Study of Lunar Volatiles” which enabled further dialogue within NASA and with COSPAR. COSPAR PPP then leveraged this scientific consensus along with multi-agency input to develop an updated COSPAR Policy on Planetary Protection in June 2021 resulting in updated mission categorizations for Earth’s Moon (existing Category II for orbiters, and new categories IIa and IIb for landed missions). NASA then updated its current planetary protection policy to apply directly to NASA and NASA partnered missions in NASA Procedural Requirements (NPR) 8715.24 entitled, “Planetary Protection Provisions for Robotic Extraterrestrial Missions”. Along with the policy update NASA’s Office of Planetary Protection has worked with mission and programmatic teams to streamline reporting requirements to a simplified checkbox and fill-in-the-blank type of template. Throughout the abovementioned process, open and transparent communication between the policy makers and implementers was essential to ensure a balance with the updated policy, scientific intent, and practicality for each mission to be responsive and achieve mission success, including Artemis I and each of its secondary payloads, Gateway, CAPSTONE and Lunar Trailblazer.

James Benardini

U.S. planetary protection program - Implementation highlights

The implementation of the United States planetary protection program, intended to prevent the contamination of the celestial bodies in the solar system during their exploration, in recent interplanetary projects is reviewed. The shift in planetary protection policy from that of absolute sterilization to a probabilistic approach is noted. Attention is then given to the use of microbiological assay, organic materials inventory, microbial burden reduction, contamination control, trajectory biasing and the analysis and identification of contaminating processes for purposed of planetary protection for the Pioneer 10 and 11, Viking and Voyager projects. The success of these measures, as demonstrated by the prevention of accidental planetary impact and the failure to detect terrestrial organisms by the Viking life detection experiments, is pointed out.

Barengoltz, J.

ISS External Microorganisms: A Payload to Close Planetary Protection Knowledge Gaps for Crewed Missions

Before NASA or COSPAR is able to set planetary protection requirements for crewed missions to locations like Mars there are a number of critical knowledge gaps that must be addressed (1). One of the most important knowledge gaps is an understanding of microbial leakage from crewed habitats and space suits. Current ECLSS (Environmental Control and Life Support System) and PLSS (Portable Life Support System) requirements do not include any provisions to control microbes that may escape along with vented or leaked gasses. The current generation of NASA space suits can leak at rates as high as 100 cm2 /min. during nominal operation (2). ISS (International Space Station) intentionally vents atmospheric gases like CO2 to maintain habitable conditions for the crew. Furthermore, every time an airlock is used for EVA (extravehicular activity)there is an accompanying release of internal atmosphere. Since it is not possible to sterilize a crewed mission, it is important that we understand what if any microbes are entrained in these vented and leaked products. It is also important to understand if these microbes can survive on exterior surfaces. Recent sampling of the Russian segments of ISS suggest that bacteria and fungi from inside ISS may be capable of surviving on external surfaces(3). NASA is developing an aseptic sampling tool for use during EVA and plans to collect samples from vents on ISS to build on these results. The results of this work will be used to develop planetary protection requirements for vented and leaked gasses from crewed volumes. NASA has developed and tested a tool kit for collecting microbiological samples during EVA(4). This tool kit contains eight commercially available, 23 mm. diameter, foam swabs that can be used to aseptically collect samples while at vacuum. The swabs are individually housed in aluminum canisters that are equipped with 0.2 μm Teflon filters. These filters allow the canisters to equilibrate to pressure changes while preventing microbiological contamination. The canisters will be cleaned and sterilized before flight. Results from ground-based testing indicate that this tool kit is capable of aseptically collecting microbes while at vacuum without becoming contaminated during pressure changes(5). Based on the results of this ground testing we have modified the tool kit to meet NASA safety requirements and improve the ergonomics. We added additional mounting points to the tool kit to give astronauts more options for securing it during use. We also changed the opening mechanism to improve the precision with which swabs can be extracted from the tool kit. We plan to use this kit on an upcoming EVA to collect samples from non-propulsive vents and areas near the U.S. airlock on ISS. These samples will be frozen at -80 ̊C and stored on station until they can be returned to Earth. We will analyze these returned samples using next generation DNA sequencing to determine the community composition and function of external ISS environments. The results of this study will close planetary protection knowledge gaps for crewed missions and will help NASA determine appropriate planetary protection requirements for life support systems. The tool kit will also be useful for collecting aseptic samples on upcoming crewed or robotic missions and could easily be modified to collect samples with organic contamination control requirements as well.

A B Regberg

ISS External Microorganisms: A Payload to Close Planetary Protection Knowledge Gaps for Crewed Missions

Before NASA or COSPAR is able to set planetary protection requirements for crewed missions to locations like Mars there are a number of critical knowledge gaps that must be addressed (1). One of the most important knowledge gaps is an understanding of microbial leakage from crewed habitats and space suits. Current ECLSS (Environmental Control and Life Support System) and PLSS (Portable Life Support System) requirements do not include any provisions to control microbes that may escape along with vented or leaked gasses. The current generation of NASA space suits can leak at rates as high as 100 cm2 /min. during nominal operation (2). ISS (International Space Station) intentionally vents atmospheric gases like CO2 to maintain habitable conditions for the crew. Furthermore, every time an airlock is used for EVA (extravehicular activity)there is an accompanying release of internal atmosphere. Since it is not possible to sterilize a crewed mission, it is important that we understand what if any microbes are entrained in these vented and leaked products. It is also important to understand if these microbes can survive on exterior surfaces. Recent sampling of the Russian segments of ISS suggest that bacteria and fungi from inside ISS may be capable of surviving on external surfaces(3). NASA is developing an aseptic sampling tool for use during EVA and plans to collect samples from vents on ISS to build on these results. The results of this work will be used to develop planetary protection requirements for vented and leaked gasses from crewed volumes. NASA has developed and tested a tool kit for collecting microbiological samples during EVA(4). This tool kit contains eight commercially available, 23 mm. diameter, foam swabs that can be used to aseptically collect samples while at vacuum. The swabs are individually housed in aluminum canisters that are equipped with 0.2 μm Teflon filters. These filters allow the canisters to equilibrate to pressure changes while preventing microbiological contamination. The canisters will be cleaned and sterilized before flight. Results from ground-based testing indicate that this tool kit is capable of aseptically collecting microbes while at vacuum without becoming contaminated during pressure changes(5). Based on the results of this ground testing we have modified the tool kit to meet NASA safety requirements and improve the ergonomics. We added additional mounting points to the tool kit to give astronauts more options for securing it during use. We also changed the opening mechanism to improve the precision with which swabs can be extracted from the tool kit. We plan to use this kit on an upcoming EVA to collect samples from non-propulsive vents and areas near the U.S. airlock on ISS. These samples will be frozen at -80 ̊C and stored on station until they can be returned to Earth. We will analyze these returned samples using next generation DNA sequencing to determine the community composition and function of external ISS environments. The results of this study will close planetary protection knowledge gaps for crewed missions and will help NASA determine appropriate planetary protection requirements for life support systems. The tool kit will also be useful for collecting aseptic samples on upcoming crewed or robotic missions and could easily be modified to collect samples with organic contamination control requirements as well.

A B Regberg

The Evolution of Planetary Protection Implementation on Mars Landed Missions

NASA has developed requirements dedicated to the prevention of forward and backward contamination during space exploration. Historically, international agreements provided guidelines to prevent contamination of the Moon and other celestial bodies, as well as the Earth (e.g., sample return missions). The UN Outer Space Treaty was established in 1967 and the Committee on Space Research (COSPAR) maintains a planetary protection policy complying with Article IX of this treaty. By avoiding forward contamination, the integrity of scientific exploration is preserved. Planetary Protection mission requirements are levied on missions to control contamination. These requirements are dependent on the science of the mission and on the celestial bodies encountered or targeted along the way. Consequently, categories are assigned to missions, and specific implementation plans are developed to meet the planetary protection requirements. NASA missions have evolved over time with increasingly more demanding scientific objectives and more complex flight systems to achieve those objectives and, thus, planetary protection methods and processes used for implementation have become much more intricate, complicated, and challenging. Here, we will portray the evolution of planetary protection implementation at JPL in several important areas throughout the course of NASA sponsored robotic Mars lander or rover missions, starting from Mars Pathfinder through the beginning of Mars 2020. Highlighted in the discussion will be process changes in planetary protection requirements development and flow down. Development and implementation of new and improved methods used in the reduction of spacecraft bioburden will be discussed as well as approaches and challenges that come along with setting up remote laboratories to perform bioassays. The consequences and forward planning of delays on missions will be highlighted as well as lessons learned on the impact of communication and training in achieving planetary protection requirements. The evolution of methods used for the detection of microbial bioburden on spacecraft hardware will be considered. These methods use standard microbiology as well as the adaptation of advances in biotechnology, molecular biology, and bioinformatics. Technical approaches developed for the prevention of contamination and recontamination of hardware during Assembly, Test, and Launch Operations will be discussed.

Kazarians, Gayane A.