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All sourcesNASA NTRSDOE OSTI
DOE OSTI2022

Impact of point spread function higher moments error on weak gravitational lensing – II. A comprehensive study

ABSTRACT Weak lensing is one of the most powerful probes for dark matter and dark energy science, although it faces increasing challenges in controlling systematic uncertainties as the statistical errors become smaller. The point spread function (PSF) needs to be precisely modeled to avoid systematic error on the weak lensing measurements. The weak lensing biases induced by errors in the PSF model second moments, i.e. its size and shape, are well-studied. However, Zhang et al. showed that errors in the higher moments of the PSF may also be a significant source of systematics for upcoming weak lensing surveys. Therefore, this work comprehensively investigate the modelling quality of PSF moments from the 3rd to 6th order, and propagate the PSFEx higher moments modelling error in the HSC survey data set to the weak lensing shear–shear correlation functions and their cosmological analyses. The overall multiplicative shear bias associated with errors in PSF higher moments can cause an ∼0.1σ shift on the cosmological parameters for LSST Y10, while the associated additive biases can induce 1σ uncertainties in cosmology parameter inference for LSST Y10, if not accounted. We compare the PSFEx model with PSF in Full FOV, and find similar performance in modelling the PSF higher moments. We conclude that PSF higher moment errors of the future PSF models should be reduced from those in current methods, otherwise needed to be explicitly modeled in the weak lensing analysis.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2023

A general framework for removing point-spread function additive systematics in cosmological weak lensing analysis

ABSTRACT Cosmological weak lensing measurements rely on a precise measurement of the shear two-point correlation function (2PCF) along with a deep understanding of systematics that affect it. In this work, we demonstrate a general framework for detecting and modelling the impact of PSF systematics on the cosmic shear 2PCF and mitigating its impact on cosmological analysis. Our framework can detect PSF leakage and modelling error from all spin-2 quantities contributed by the PSF second and higher moments, rather than just the second moments, using the cross-correlations between galaxy shapes and PSF moments. We interpret null tests using the HSC Year 3 (Y3) catalogs with this formalism and find that leakage from the spin-2 combination of PSF fourth moments is the leading contributor to additive shear systematics, with total contamination that is an order-of-magnitude higher than that contributed by PSF second moments alone. We conducted a mock cosmic shear analysis for HSC Y3 and find that, if uncorrected, PSF systematics can bias the cosmological parameters Ωm and S8 by ∼0.3σ. The traditional second moment-based model can only correct for a 0.1σ bias, leaving the contamination largely uncorrected. We conclude it is necessary to model both PSF second and fourth moment contaminations for HSC Y3 cosmic shear analysis. We also reanalyse the HSC Y1 cosmic shear analysis with our updated systematics model and identify a 0.07σ bias on Ωm when using the more restricted second moment model from the original analysis. We demonstrate how to self-consistently use the method in both real space and Fourier space, assess shear systematics in tomographic bins, and test for PSF model overfitting.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗
NASA NTRS1996

Searching for Faint Companions to Nearby Stars with the Hubble Space Telescope

A search for faint companions (FC's) to selected stars within 5 pc of the Sun using the Hubble Space Telescope's Planetary Camera (PC) has been initiated. To assess the PC's ability to detect FCs, we have constructed both model and laboratory-simulated images and compared them to actual PC images. We find that the PC's point-spread function (PSF) is 3-4 times brighter over the angular range 2-5 sec than the PSF expected for a perfect optical system. Azimuthal variations of the PC's PSF are 10-20 times larger than expected for a perfect PSF. These variations suggest that light is scattered nonuniformly from the surface of the detector. Because the anomalies in the PC's PSF cannot be precisely simulated, subtracting a reference PSF from the PC image is problematic. We have developed a computer algorithm that identifies local brightness anomalies within the PSF as potential FCs. We find that this search algorithm will successfully locate FCs anywhere within the circumstellar field provided that the average pixel signal from the FC is at least 10 sigma above the local background. This detection limit suggests that a comprehensive search for extrasolar Jovian planets with the PC is impractical. However, the PC is useful for detecting other types of substellar objects. With a stellar signal of 10(exp 9) e(-), for example, we may detect brown dwarfs as faint as M(sub I) = 16.7 separated by 1 sec from alpha Cen A.

Schroeder, Daniel J.↗
NASA NTRS1999

Point Spread Function and Transmittance Analyses for Conical and Hexapod Secondary Mirror Support Towers for the Next Generation Space Telescope (NGST)

Different secondary mirror support towers were modeled on the CODE V optical design/analysis program for the NGST Optical Telescope Assembly (OTA) B. The vertices of the NGST OTA B primary and secondary mirrors were separated by close to 9.0 m. One type of tower consisted of a hollow cone 6.0 m long, 2.00 m in diameter at the base, and 0.704 m in diameter at its top. The base of the cone was considered attached to the primary's reaction structure through a hole in the primary. Extending up parallel to the optical axis from the top of this cylinder were eight blades (pyramidal struts) 3.0 m long. A cross section of each these long blades was an isosceles triangle with a base of 0.010 m and a height of 0.100 m with the sharpest part of each triangle pointing inward. The eight struts occurred every 45 deg. The other type of tower was purely a hexapod arrangement and had no blades or cones. The hexapod consisted simply of six, very thin, circular struts, leaving in pairs at 12:00, 4:00, and 8:00 at the primary and traversing to the outer edge of the back of the secondary mount. At this mount, two struts arrived at each of 10:00, 2:00, and 6:00. The struts were attached to the primary mirror in a ring 3.5 m in diameter. They reached the back of the secondary mount, a circle 0.704 m in diameter. Transmittance analyses at two levels were performed on the secondary mirror support towers. Detailed transmittances were accomplished by the use of the CODE V optical design/analysis program and were compared to transmittance calculations that were almost back-of-the-envelope. Point spread function (PSF) calculations, including both diffraction and aberration effects, were performed on CODE V. As one goes out from the center of the blur (for a point source), the two types of support towers showed little difference between their PSF intensities until one reaches about the 3% level. Contours can be delineated on CODE V down to about 10 (exp -8) times the peak intensity, fine detailing the differences in the wings of the PSF. Between the first type of tower and the hexapod tower, below 3% of the peak intensity, the PSF contour maps well delineated significant differences in the outer portions of the PSFS. Such differences in the wings of the PSF significantly impact the resolution of brown dwarfs or extra solar planets from their star. The hexapod tower provided the best PSF.

Wilkerson, Gary W.↗
DOE OSTI2024

Polydiallylammonium-Polysulfone Multiblock Copolymers for Moisture-Swing Direct Air Capture of Carbon Dioxide

Polysulfones are an important class of materials for a variety of applications due to their excellent strength and thermal stability. Quaternary ammonium polymers are also useful materials due to their ion exchange properties, and such materials with alkaline counterions have been used to capture carbon dioxide (CO 2 ) from ambient air through a moisture-driven mechanism. Herein, we design sulfone-based multiblock copolymers containing ammonium functionalities and demonstrate their potential for CO 2 capture. Specifically, a series of multiblock copolymers containing both polydiallyldimethylammonium (PDADMA) and polysulfone (PSf) blocks were synthesized. PSf blocks provide mechanical integrity, while the PDADMA blocks enable the direct air capture of CO 2 . Multiblock copolymers were synthesized at high yield, and PDADMA(OH)-PSf copolymer films with good flexibility and strength were formed with IECs of 424 μmol/g (17 mol % PDADMA(OH)), 1407 μmol/g (44 mol % PDADMA(OH)), and 1726 μmol/g (50 mol % PDADMA(OH)). Thermogravimetric analysis was used to determine that all films are thermally stable up to 345 °C. Differential scanning calorimetry revealed that the 17 and 44 mol % functionalized PDADMA(OH)-PSf multiblock copolymers showed one T g at 185 °C and 50 mol % PDADMA(OH)-PSf polymer showed a T g at 183 °C and a second T g at 16 °C. Atomic force microscopy showed that the multiblock polymers exhibit disordered phase separation. Furthermore, the copolymer materials displayed moisture-swing direct air capture of CO 2 , opening up pathways to utilize polymer architecture and chemistry to tailor the properties of promising sorbents.

42 ENGINEERING↗
DOE OSTI2022

TDCOSMO. IX. Systematic comparison between lens modelling software programs: time delay prediction for WGD 2038-4008

The importance of alternative methods for measuring the Hubble constant, such as time-delay cosmography, is highlighted by the recent Hubble tension. It is paramount to thoroughly investigate and rule out systematic biases in all measurement methods before we can accept new physics as the source of this tension. In this study, we perform a check for systematic biases in the lens modelling procedure of time-delay cosmography by comparing independent and blind time-delay predictions of the system WGD 2038-4008 from two teams using two different software programs: GLEE and LENSTRONOMY. The predicted time delays from the two teams incorporate the stellar kinematics of the deflector and the external convergence from line-of-sight structures. The un-blinded time-delay predictions from the two teams agree within 1.2σ, implying that once the time delay is measured the inferred Hubble constant will also be mutually consistent. However, there is a ~4σ discrepancy between the power-law model slope and external shear, which is a significant discrepancy at the level of lens models before the stellar kinematics and the external convergence are incorporated. We identify the difference in the reconstructed point spread function (PSF) to be the source of this discrepancy. When the same reconstructed PSF was used by both teams, we achieved excellent agreement, within ~0.6σ<, indicating that potential systematics stemming from source reconstruction algorithms and investigator choices are well under control. We recommend that future studies supersample the PSF as needed and marginalize over multiple algorithms or realizations for the PSF reconstruction to mitigate the systematics associated with the PSF. A future study will measure the time delays of the system WGD 2038-4008 and infer the Hubble constant based on our mass models.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2025

Dark Energy Survey Year 6 Results: Point-Spread Function Modeling

We present the point-spread function (PSF) modeling for weak lensing shear measurement using the full six years of the Dark Energy Survey (DES Y6) data. We review the PSF estimation procedure using the PIFF (PSFs In the Full FOV) software package and describe the key improvements made to PIFF and modeling diagnostics since the DES year three (Y3) analysis: (i) use of external Gaia and infrared photometry catalogs to ensure higher purity of the stellar sample used for model fitting, (ii) addition of color-dependent PSF modeling, the first for any weak lensing analysis, and (iii) inclusion of model diagnostics inspecting fourth-order moments, which can bias weak lensing measurements to a similar degree as second-order modeling errors. Through a comprehensive set of diagnostic tests, we demonstrate the improved accuracy of the Y6 models evident in significantly smaller systematic errors than those of the Y3 analysis, in which all g band data were excluded due to insufficiently accurate PSF models. For the Y6 weak lensing analysis, we include g band photometry data in addition to the riz bands, providing a fourth band for photometric redshift estimation. Looking forward to the next generation of wide-field surveys, we describe several ongoing improvements to PIFF, which will be the default PSF modeling software for weak lensing analyses for the Vera C. Rubin Observatory’s Legacy Survey of Space and Time.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
NASA NTRS1983

Estimation of a remote sensing system point-spread function from measured imagery

A general approach to identifying the point spread function (PSF) of a remotely sensed scene is demonstrated in terms of a step function for an abrupt change in the gray level along the row or column of the image data. The estimate of the PSF is made in terms of a finite sum of basis functions, employing a sequence of rectangular pulses covering the spatial extent of the PSF. The approximation, if narrow impulses are employed, provides accurate fidelity to the PSF. The method becomes practical when the geometrical structure of the scene elements producing the measured response is known. The field boundary is obtained through consideration of the differing intensities on each side of the boundary, which is a step discontinuity. The mathematical procedure for the technique is provided, together with a sample problem from Landsat-4 Thematic Mapper data. Atmospheric blurring and electronic effects on the overall PSF and the cubic convolution resampling effects are noted.

Mcgillem, C. D.↗
NASA NTRS1988

Effect of central obscuration on the LDR point spread function

It is well known that Gaussian apodization of an aperture reduces the sidelobe levels of its point spread function (PSF). In the limit where the standard deviation of the Gaussian function is much smaller than the diameter of the aperture, the sidelobes completely disappear. However, when Gaussian apodization is applied to the Large Deployable Reflector (LDR) array consisting of 84 hexagonal panels, it is found that the sidelobe level only decreases by about 2.5 dB. The reason for this is explained. The PSF is shown for an array consisting of 91 uniformly illuminated hexagonal apertures; this array is identical to the LDR array, except that the central hole in the LDR array is filled with seven additional panels. For comparison, the PSF of the uniformly illuminated LDR array is shown. Notice that it is already evident that the sidelobe structure of the LDR array is different from that of the full array of 91 panels. The PSF's of the same two arrays are shown, but with the illumination apodized with a Gaussian function to have 20 dB tapering at the edges of the arrays. While the sidelobes of the full array have decreased dramatically, those of the LDR array changed in structure, but stayed at almost the same level. This result is not completely surprising, since the Gaussian apodization tends to emphasize the contributions from the central portion of the array; exactly where the hole in the LDR array is located. The two most important conclusions are: the size of the central hole should be minimized, and a simple Gaussian apodization scheme to suppress the sidelobes in the PSF should not be used. A more suitable apodization scheme would be a Gaussian annular ring.

Vanzyl, Jakob J.↗
NASA NTRS1993

X-ray evidence for particulate contamination on the AXAF VETA-1 mirrors

X-ray testing of the AXAF outer mirror pairs, in the VETA-1 configuration, reveals a point spread function (PSF) with unexpectedly large wings at low energies. Although the angular dependence in the wings of the PSF is close to that expected for diffractive scattering from surface roughness, the energy dependence differs substantially. Analyses of the observed X-ray PSF, images near ring focus, and single-quadrant images at conjugate focus suggest that the excess scattering observed at low X-ray energies results from diffractive scattering by relatively small grains (as small as a few tenths micrometer in radius). We develop a simple model for the contribution of scattering by particulates to the PSF. Merging this model with that for scattering by surface roughness, we fit the combined model to the observed energy-dependent PSF, in order to estimate parameters and associated uncertainties characterizing the grain-size distribution and the surface-roughness power spectral density. In particular, we find that the fractional coverage of the mirrors by particulates is approximately 1 x 10 exp -4 (for grain radii between 0.1 and 10 microns), and that the rms surface-roughness is approximately 0.7 nm (for spatial frequencies between 1/mm and 1000/mm).

O'Dell, S. L.↗
NASA NTRS1999

Point Response Characteristics for the CERES/EOS-PM, FM3 & FM4 instruments.

This paper describes the point source functions (PSF s) of the Clouds and the Earth s Radiant Energy System (CERES,) Earth Observing System (EOS,) afternoon platform (PM,) Flight Model 3 (FM3,) and Flight Model 4 (FM4) scanning instruments. The PSF (also known as the Point Response Function, or PRF) is vital to the accurate geo-location of the remotely sensed radiance measurements acquired by the instrument. This paper compares the characteristics of the FM3 and FM4 instruments with the earlier Proto Flight Model (PFM) on the Tropical Rainfall Measuring Mission (TRMM) platform, and the FM1 and FM2 Models on the EOS morning orbiting (AM) platform, which has recently been renamed "Terra". All of the PSF s were found to be quite comparable, and the previously noted "spreading" characteristic of the window (water vapor) channel PSF is analyzed Keywords: PSF, PRF, CERES, TRMM, EOS, Earth Radiation Budget

Paden, Jack↗
NASA NTRS2005

Quickbird Satellite in-orbit Modulation Transfer Function (MTF) Measurement Using Edge, Pulse and Impulse Methods for Summer 2003

The spatial characteristics of an imaging system cannot be expressed by a single number or simple statement. However, the Modulation Transfer Function (MTF) is one approach to measure the spatial quality of an imaging system. Basically, MTF is the normalized spatial frequency response of an imaging system. The frequency response of the system can be evaluated by applying an impulse input. The resulting impulse response is termed the Point Spread function (PSF). This function is a measure of the amount of blurring present in the imaging system and is itself a useful measure of spatial quality. An underlying assumption is that the imaging system is linear and shift-independent. The Fourier transform of the PSF is called the Optical Transfer Function (OTF) and the normalized magnitude of the OTF is the MTF. In addition to using an impulse input, a knife-edge in technique has also been used in this project. The sharp edge exercises an imaging system at all spatial frequencies. The profile of an edge response from an imaging system is called an Edge Spread Function (ESF). Differentiation of the ESF results in a one-dimensional version of the Point Spread Function (PSF). Finally, MTF can be calculated through use of Fourier transform of the PSF as stated previously. Every image includes noise in some degree which makes MTF of PSF estimation more difficult. To avoid the noise effects, many MTF estimation approaches use smooth numerical models. Historically, Gaussian models and Fermi functions were applied to reduce the random noise in the output profiles. The pulse-input method was used to measure the MTF of the Landsat Thematic Mapper (TM) using 8th order even functions over the San Mateo Bridge in San Francisco, California. Because the bridge width was smaller than the 30-meter ground sample distance (GSD) of the TM, the Nyquist frequency was located before the first zero-crossing point of the sinc function from the Fourier transformation of the bridge pulse. To avoid the zero-crossing points in the frequency domain from a pulse, the pulse width should be less than the width of two pixels (or 2 GSD's), but the short extent of the pulse results in a poor signal-to-noise ratio. Similarly, for a high-resolution satellite imaging system such as Quickbird, the input pulse width was critical because of the zero crossing points and noise present in the background area. It is important, therefore, that the width of the input pulse be appropriately sized. Finally, the MTF was calculated by taking ratio between Fourier transform of output and Fourier transform of input. Regardless of whether the edge, pulse and impulse target method is used, the orientation of the targets is critical in order to obtain uniformly spaced sub-pixel data points. When the orientation is incorrect, sample data points tend to be located in clusters that result in poor reconstruction of the edge or pulse profiles. Thus, a compromise orientation must be selected so that all spectral bands can be accommodated. This report continues by outlining the objectives in Section 2, procedures followed in Section 3, descriptions of the field campaigns in Section 4, results in Section 5, and a brief summary in Section 6.

Helder, Dennis↗
NASA NTRS2010

A Point Spread Function for the EPOXI Mission

The Extrasolar Planet Observation Characterization and the Deep Impact Extended Investigation missions (EPOXI) are currently observing the transits of exoplanets, two comet nuclei at short range, and the Earth and Mars using the High Resolution Instrument (HRI) - a 0.3 m f/35 telescope on the Deep Impact probe. The HRI is in a permanently defocused state with the instrument pOint of focus about 0.6 cm before the focal plane due to the use of a reference flat mirror that took a power during ground thermal-vacuum testing. Consequently, the point spread function (PSF) covers approximately nine pixels FWHM and is characterized by a patch with three-fold symmetry due to the three-point support structures of the primary and secondary mirrors. The PSF is also strongly color dependent varying in shape and size with change in filtration and target color. While defocus is highly desirable for exoplanet transit observations to limit sensitivity to intra-pixel variation, it is suboptimal for observations of spatially resolved targets. Consequently, all images used in our analysis of such objects were deconvolved with an instrument PSF. The instrument PSF is also being used to optimize transit analysis. We discuss development and usage of an instrument PSF for these observations.

Barry, Richard K.↗
DOE OSTI2022

The HUNTER Dynamic Human Reliability Analysis Tool: Development of a Module for Performance Shaping Factors

Human Unimodel for Nuclear Technology to Enhance Reliability (HUNTER) is a framework to support dynamic human reliability analysis (HRA) in communication with a variety of methods and tools. In this paper, how we have developed one of the HUNTER modules, the individual module for evaluating performance shaping factors (PSFs), is introduced. The PSF refers to any factor that influences human performance such as workload or complexity. It has been used for highlighting human errors and adjusting the error probabilities in the existing HRA. We consider the eight PSFs suggested in the Standardized Plant Analysis Risk-HRA (SPAR-H) method, which is the representative HRA method widely used in the nuclear field. To support our dynamic modeling using the eight SPAR-H PSFs, we reviewed human performance literature and developed data-based mathematical models to rate and quantify PSFs in the context of dynamic HRA. We also design the individual module composed of the two functions: 1) the PSF qualification function that automatically or manually evaluates a PSF level, and 2) the PSF quantification function that dynamically or statically determines the PSF multiplier values and integrate them to adjust human error probabilities (HEPs). How each function works with the SPAR-H PSFs and how the PSFs adjust the HEPs are investigated through literature and discussed in this paper.

99 GENERAL AND MISCELLANEOUS↗
DOE OSTI2022

The HUNTER Dynamic Human Reliability Analysis Tool: Development of a Module for Performance Shaping Factors

The Human Unimodel for Nuclear Technology to Enhance Reliability (HUNTER) is a framework to support dynamic human reliability analysis (HRA) in communication with a variety of methods and tools. This paper explores how we developed one of the HUNTER modules: namely, the Individual module for evaluating performance shaping factors (PSFs). A PSF is any factor that influences human performance (e.g., workload or complexity). In the existing HRA, they are used to highlight human errors and adjust error probabilities. We consider the eight PSFs suggested in the Standardized Plant Analysis Risk-HRA (SPAR-H) method, a representative HRA method widely used in the nuclear field. To support our dynamic modeling using the eight SPAR-H PSFs, we reviewed the human performance literature and developed data-based mathematical models to rate and quantify PSFs in the context of dynamic HRA. We also designed the Individual module to consist of two functions: (1) the PSF qualification function for automatically or manually evaluating PSF levels, and (2) the PSF quantification function for dynamically or statically determining PSF multiplier values and integrating them to adjust human error probabilities (HEPs). How each function works in regard to the SPAR-H PSFs, and how the PSFs serve to adjust the HEPs, were investigated via literature review and are discussed in this paper.

99 GENERAL AND MISCELLANEOUS↗
DOE OSTI2023

Redshifting galaxies from DESI to JWST CEERS: Correction of biases and uncertainties in quantifying morphology

Observations of high-redshift galaxies with unprecedented detail have now been rendered possible with the James Webb Space Telescope (JWST). However, accurately quantifying their morphology remains uncertain due to potential biases and uncertainties. To address this issue, we used a sample of 1816 nearby DESI galaxies, with a stellar mass range of 10 9.75 - 11.25 M ⊙ , to compute artificial images of galaxies of the same mass located at 0.75 ≤ z ≤ 3 and observed at rest-frame optical wavelength in the Cosmic Evolution Early Release Science (CEERS) survey. We analyzed the effects of cosmological redshift on the measurements of Petrosian radius (R p ), half-light radius (R 50 ), asymmetry (A), concentration (C), axis ratio (q), and Sérsic index (n). Our results show that R p and R 50 , calculated using non-parametric methods, are slightly overestimated due to PSF smoothing, while R 50 , q, and n obtained through fitting a Sérsic model does not exhibit significant biases. By incorporating a more accurate noise effect removal procedure, we improve the computation of A over existing methods, which often overestimate, underestimate, or lead to significant scatter of noise contributions. Due to PSF asymmetry, there is a minor overestimation of A for intrinsically symmetric galaxies. However, for intrinsically asymmetric galaxies, PSF smoothing dominates and results in an underestimation of A, an effect that becomes more significant with higher intrinsic A or at lower resolutions. Moreover, PSF smoothing also leads to an underestimation of C, which is notably more pronounced in galaxies with higher intrinsic C or at lower resolutions. We developed functions based on resolution level, defined as R p /FWHM, for correcting these biases and the associated statistical uncertainties. Applying these corrections, we measured the bias-corrected morphology for the simulated CEERS images and we find that the derived quantities are in good agreement with their intrinsic values – except for A, which is robust only for angularly large galaxies where R p /FWHM ≥ 5. Our correction functions can be applied to other surveys, offering valuable tools for future studies.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2025

Chasing ghosts: characterization of artifact generation in coded aperture decoding due to experimental implementation

Coded aperture imaging is a form of lensless aperture imaging that projects multiple overlapping images of the source onto the detector, enhancing signal strength, which is advantageous for low-flux sources or high-resolution imaging. This technique requires decoding of the detector signal to reconstruct the original source, which involves convolving the detector data with the aperture pattern. When the signal is from a centered point source, the reconstructed source image is known as the point spread function (PSF). A clean PSF without artifacts is a Dirac delta function [Appl. Opt. 20, 1858 (1981)]. This paper examines the robustness of the decoding process against variations in experimental tolerances by analyzing artifact growth in the reconstructed PSF. We illustrate the effects of incorrect magnification, rotation, and detector size and find that aperture–detector rotational misalignment about the imaging axis is the most sensitive parameter, with significant artifact generation occurring with angular offsets of less than one degree. We discuss compensation methods for imperfect aperture placement, finding that small detector sizes produce uncompensatable artifact generation, and compare theoretical predictions with experimental PSF measurements of a rank , 6.8 mm thick (less than one mean free path) coded aperture with a 3.5 mm cell size, conducted at the MegaJOuLe Neutron Imaging Radiography dense plasma focus [IEEE Trans. Plasma Sci. 49, 3299 (2021)] using a 2.45 MeV neutron source. Based on our findings, we recommend using magnified coded apertures in the under-sampled regime, which allows for the inclusion of fiducial markers to characterize aperture–detector rotational offsets and the addition of mechanical coupling, where possible, to constrain rotational and magnification offsets.

Selwood, M. P. [Lawrence Livermore National Labora↗
DOE OSTI2025

Transcriptomic and metabolomic analysis of recalcitrant phosphorus solubilization mechanisms in Trametes gibbosa

Introduction Phosphorus (P) is a crucial growth-limiting nutrient in soil, much of which remains challenging for plants to absorb and use. Unlike chemical phosphate fertilizers, phosphate-solubilizing microorganisms (PSMs) offer a means to address available phosphorus deficiency without causing environmental harm. PSMs possess multiple mechanisms for phosphorus solubilization. Although the phosphorus-solubilizing mechanisms of phosphate-solubilizing bacteria (PSB) have been well characterized, the mechanisms utilized by phosphate-solubilizing fungi (PSF) remain largely unexplored. Methods This study isolated a PSF strain, Trametes gibbosa T-41, from soil and evaluated its phosphorus solubilizing capacity with organic (calcium phytin; Phytin-P) and inorganic (tricalcium phosphate; Ca-P) phosphorus sources. The phosphorus solubilization, enzyme activity, and organic acid production of T-41 were measured. And the P-solubilizing mechanism conducted by transcriptomic and metabolomic analyses. Results and discussion T-41 exhibited varying phosphorus solubilizing capacity when grown with organic (calcium phytin; Phytin-P) and inorganic (tricalcium phosphate; Ca-P) phosphorus sources (109.80 ± 8.9 mg/L vs. 57.5 ± 7.9 mg/L, p < 0.05). Compared with the Ca-P treatment, T-41 demonstrated a stronger alkaline phosphatase (ALP) production capacity under Phytin-P treatment (34.5 ± 1.2 μmol/L/h vs. 19.8 ± 0.8 μmol/L/h, p < 0.05). Meanwhile, the production of oxalic acid, maleic acid, and succinic acid was higher under Phytin-P treatment ( p < 0.05). Transcriptomic and metabolomic analysis revealed that different phosphorus sources altered metabolic pathways such as galactose metabolism, glyoxylate and dicarboxylic acid metabolism, and ascorbate and aldolate metabolism. Key metabolites like myo-inositol, 2-oxoglutarate, and pyruvate were found to impact the performance of T. gibbosa T-41 differently under the two P sources. Notably, synthesis in Ca-P vs. Pytin-P, T-41 upregulated genes involved in myo-inositol synthesis, potentially enhancing its P-solubilizing ability. These results provide new insights into the molecular mechanisms of PSF at the transcriptomic and metabolomic levels, laying a theoretical foundation for the broader application of PSF as bio-phosphorus fertilizers in the future.

Chen, Yulan↗