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At least 37 records · Page 2

The luminosity function for different morphological types in the CfA Redshift Survey

We derive the luminosity function for different morphological types in the original CfA Redshift Survey (CfA1) and in the first two slices of the CfA Redshift Survey Extension (CfA2). CfA1 is a complete sample containing 2397 galaxies distributed over 2.7 steradians with m(sub z) less than or equal 14.5. The first two complete slices of CfA2 contain 1862 galaxies distributed over 0.42 steradians with m(sub z)=15.5. The shapes of the E-S0 and spiral luminosity functions (LF) are indistinguishable. We do not confirm the steeply decreasing faint end in the E-S0 luminosity function found by Loveday et al. for an independent sample in the southern hemisphere. We demonstrate that incomplete classification in deep redshift surveys can lead to underestimates of the faint end of the elliptical luminosity function and could be partially responsible for the difference between the CfA survey and other local field surveys. The faint end of the LF for the Magellanic spirals and irregulars is very steep. The Sm-Im luminosity function is well fit by a Schechter function with M*=-18.79, alpha=-1.87, and phi*=0.6x10(exp -3) for M(sub z) less than or equal to -13. These galaxies are largely responsible for the excess at the faint end of the general CfA luminosity function. The abundance of intrinsically faint, blue galaxies nearby affects the interpretation of deep number counts. The dwarf population increases the expected counts at B=25 in a no-evolution, q(sub 0)=0.05 model by a factor of two over standard no-evolution estimates. These dwarfs change the expected median redshift in deep redshift surveys by less than 10 percent . Thus the steep Sm-Im LF may contribute to the reconciliation of deep number counts with deep redshift surveys.

Marzke, Ronald O.↗

A Search for Neutrino Point-source Populations in 7 yr of IceCube Data with Neutrino-count Statistics

The presence of a population of point sources in a data set modifies the underlying neutrino-count statistics from the Poisson distribution. This deviation can be exactly quantified using the non-Poissonian template fitting technique, and in this work we present the first application of this approach to the IceCube high-energy neutrino data set. Using this method, we search in 7 yr of IceCube data for point-source populations correlated with the disk of the Milky Way, the Fermi bubbles, the Schlegel, Finkbeiner, and Davis dust map, or with the isotropic extragalactic sky. No evidence for such a population is found in the data using this technique, and in the absence of a signal, we establish constraints on population models with source-count distribution functions that can be described by a power law with a single break. Here, the derived limits can be interpreted in the context of many possible source classes. In order to enhance the flexibility of the results, we publish the full posterior from our analysis, which can be used to establish limits on specific population models that would contribute to the observed IceCube neutrino flux.

79 ASTRONOMY AND ASTROPHYSICS↗

ATP as a biomarker of viable microorganisms in clean-room facilities

A new firefly luciferase bioluminescence assay method that differentiates free extracellular ATP (dead cells, etc.) from intracellular ATP (viable microbes) was used to determine the viable microbial cleanliness of various clean-room facilities. For comparison, samples were taken from both clean-rooms, where the air was filtered to remove particles >0.5 microm, and ordinary rooms with unfiltered air. The intracellular ATP was determined after enzymatically degrading the sample's free ATP. Also for comparison, cultivable microbial populations were counted on nutrient-rich trypticase soy agar (TSA) plates. Both the cultivable and ATP-based determinations indicate that the microbial burden was lower in clean-room facilities than in ordinary rooms. However, there was no direct correlation between the two sets of measurements because the two assays measured very different populations. A large fraction of the samples yielded no colony formers on TSA, but were positive for intracellular ATP. Subsequently, genomic DNA was isolated directly from selected samples and 16S rDNA fragments were cloned and sequenced, identifying nearest neighbors, many of which are known to be noncultivable in the media employed. It was concluded that viable microbial contamination can be reliably monitored by measurement of intracellular ATP, and that this method may be considered superior to cultivable colony counts due to its speed and its ability to report the presence of viable but noncultivable organisms. When the detection of nonviable microbes is of interest, the ATP assay can be supplemented with DNA analysis.

NASA Discipline Environmental Health↗

Axenisation of oleaginous microalgal cultures via anoxic photosensitisation

Growing interest in sustainable food and biofuel research has necessitated high quality axenic oleaginous microalgal strains. Unfortunately, most strains available in culture banks contain commensal microbes such as bacteria and the default decontamination method involves antibiotic treatment which has begun to exacerbate the emergence of antibiotic resistance. To overcome this problem, anoxic photosensitisation was investigated as an alternate approach. Four oleaginous microalgal species (Tetradesmus obliquus, Desmodesmus armatus, Chlorella vulgaris and Nannochloropsis limnetica) were incubated in varying concentrations of Rose Bengal (0 μM, 1 μM, 3 μM or 9 μM) either in normal (oxic) or anoxic conditions, for 72 h under light (8.85 ± 0.4 W/m 2 ) in a specially designed heterotrophic growth complex (HGC) medium, followed by 72 h in standard Bold's Basal Medium (BBM). Commonly used antibiotics-based protocol was used as the control method. Post treatment, cell numbers and percentage populations were counted with Flow Cytometry, and viability was tested using standard plating methods using BBM and LB. Additionally, the contaminating microbes in the cultures were profiled using 16Ss rRNA sequencing. Anoxic conditions were able to significantly decrease bacterial content, albeit with an equally detrimental effect on the microalgal population. Although the responses differed between the microalgae, anoxic incubation along with Rose Bengal at 3 μM was able to completely decontaminate N. limnetica and C. vulgaris, while D. armatus and T. obliquus could be decontaminated with an additional streak-plating step. None of the cultures could be decontaminated using antibiotics treatment. These results suggest that axenisation of microalgal cultures was largely due to anoxy, that was synergistically enhanced by Rose Bengal at a concentration of ≥3 μM.

59 BASIC BIOLOGICAL SCIENCES↗

Ion Storage Tests with the High Performance Antimatter Trap (HiPAT)

The NASA/Marshall Space Flight Centers (NASA/MSFC) Propulsion Research Center (PRC) is evaluating an antiproton storage system, referred to as the High Performance Antiproton Trap (HiPAT). This interest stems from the sheer energy represented by matter/antimatter annihilation process with has an energy density approximately 10 order of magnitude above that of chemical propellants. In other terms, one gram of antiprotons contains the equivalent energy of approximately 23 space shuttle external tanks or ET's (each ET contains roughly 740,000 kgs of fuel and oxidizer). This incredible source of stored energy, if harnessed, would be an enabling technology for deep space mission where both spacecraft weight and propulsion performance are key to satisfying aggressive mission requirements. The HiPAT hardware consists of a 4 Tesla superconductor system, an ultra high vacuum test section (vacuum approaching 10(exp -12) torr), and a high voltage confinement electrode system (up to 20 kvolts operation). The current laboratory layout is illustrated. The HiPAT designed objectives included storage of up to 1 trillion antiprotons with corresponding lifetimes approaching 18 days. To date, testing has centered on the storage of positive hydrogen ions produced in situ by a stream of high-energy electrons that passes through the trapping region. However, due to space charge issues and electron beam compression as it passes through the HiPAT central field, current ion production is limited to less then 50,000 ions. Ion lifetime was determined by counting particle populations at the end of various storage time intervals. Particle detection was accomplished by destructively expelling the ions against a micro-channel plate located just outside the traps magnetic field. The effect of radio frequency (RF) stabilization on the lifetime of trapped particles was also examined. This technique, referred to as a rotating wall, made use of a segmented electrode located near the center of the trap on which various phases of a particular frequency were applied. Various experiments were performed illustrating the ability of an RF drive to both prolong and reduced the lifetimes of various ion species depending on the selected frequency. HiPAT is now being reconfigured for testing with an ion source that will provide both positive and negative hydrogen ions from an external source. This ion system shall provide higher fill capacity (order of million of ions per shot), stacking of multiple shots, and injection schemes typical of a realistic antiproton delivery system.

Martin, James J.↗

Microspheres

Vital information on a person's physical condition can be obtained by identifying and counting the population of T-cells and B-cells, lymphocytes of the same shape and size that help the immune system protect the body from the invasion of disease. The late Dr. Alan Rembaum developed a method for identifying the cells. The method involved tagging the T-cells and B-cells with microspheres of different fluorescent color. Microspheres, which have fluorescent dye embedded in them, are chemically treated so that they can link with antibodies. With the help of a complex antibody/antigen reaction, the microspheres bind themselves to specific 'targets,' in this case the T-cells or B-cells. Each group of cells can then be analyzed by a photoelectronic instrument at different wavelengths emitted by the fluorescent dyes. Same concept was applied to the separation of cancer cells from normal cells. Microspheres were also used to conduct many other research projects. Under a patent license Magsphere, Inc. is producing a wide spectrum of microspheres on a large scale and selling them worldwide for various applications.

Source record↗

LandScan HD

Research in geographic information science and technology at Oak Ridge National Laboratory is addressing the need for improved human settlement detection and characterization at the world scale.

58 GEOSCIENCES↗

Comparison of Approach-to-Critical Results in Current and Pulse Mode for Systems with High Starter Neutron Rates

Reactors and critical assemblies use a variety of detection systems to monitor the neutron population. The count rate is proportional to the neutron flux present at the location of the detector. When such systems are placed external to an assembly, it is often assumed that the relative leakage multiplication will be proportional to the detector count rate (assuming that the source term, system geometry, and detector placement have not changed). Such systems are often used in an approach-to-critical during reactor startup to ensure that the critical configuration is well predicted. Various types of detectors have been used during an approach-to-critical. These include 3 He, BF 3 , ion chambers, fission chambers, and fission foils. Any of these types of systems (or others) should work well when adequate counting statistics are available. These detector systems can be operated in either pulse or current mode. The National Criticality Experiments Research Center (NCERC) has two detection systems that are commonly used in critical assembly operations. The start-up (referred to as "SU" in this work) system is made up of 3 He proportional counters in pulse mode and the linear counter system (referred to as "LC" in this work) consists of compensated ion-chambers in current mode. Typically the SU system is used for approach-to-critical operations and the LC system is only used at/above delayed critical ( k eff = 1). This work investigates the use of the LC system for an approach-to-critical. It has been long hypothesized that such an approach would be feasible for systems with high starter neutron rates.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

LandScan mosaic enables high-resolution gridded population estimates with explicit uncertainty

Gridded population datasets represent high-resolution distributions of human occupancy, enabling informed decision-making across a broad range of fields. These data products are valuable for assessing environmental risk, urban development, disaster preparedness and resource allocation—areas where accurate population estimates directly enhance policy effectiveness and optimize resource distribution. Despite the importance of gridded population datasets, traditional population modeling approaches often overlook inherent uncertainties in the estimation process. This limitation can create a false sense of certainty in population estimates, potentially leading to flawed decisions by those who rely on the data. To address this methodological gap, we introduce a probabilistic machine learning modeling framework, LandScan Mosaic, that explicitly incorporates uncertainty into the population modeling process. Our approach systematically quantifies uncertainty in three key modeling parameters of the LandScan HD gridded population dataset: building use types, floor counts, and occupancy rates. By employing Monte Carlo simulations, we propagate these uncertainties through the modeling process, yielding probability distributions of population counts in place of deterministic point estimates. We demonstrate the practical application of this framework in Iloilo City, Philippines, using structured decision-making techniques and our probabilistic estimates to identify and prioritize areas most affected by projected flooding, supporting targeted interventions that address both economic and social risks. In doing so, we propose a population-specific approach for incorporating confidence into structured decision making processes. Through a comparative analysis with conventional deterministic approaches and point estimate approaches, including LandScan HD and WorldPop, we evaluate how the incorporation of machine learning and uncertainty influences decision rankings. This research advances population distribution modeling by offering a robust, quantitative approach that explicitly accounts for uncertainty in the underlying data, along with guidance for how users can apply uncertainty in their decision-making.

Environmental sciences↗

Male greater sage‐grouse movements among leks

ABSTRACT Movements among leks by breeding birds (i.e., interlek movements) could affect the population's genetic flow, complicate use of lek counts as a population index, and indicate a change in breeding behavior following a disturbance. We used a Bayesian multi‐state mark‐recapture model to assess the daily probability of male greater sage‐grouse ( Centrocercus urophasianus ) interlek movements and estimate factors influencing movements among leks. We fitted 145 males with solar Argos global positioning systems platform transmitter terminals over 4 years (2011–2014) in Carbon County, Wyoming, USA. The daily probability of a male sage‐grouse moving among leks ranged 0.003 (95% CI = 0.000–0.010) in 2011 to 0.010 (95% CI = 0.001–0.021) in 2013, indicating high daily lek fidelity throughout the season, although there was a 5–42% chance annually a male would move at least once to another lek throughout the season ( days analyzed/M = 55 ± 3.3 days [SE]). Interlek movement probabilities were strongly affected by day of year, peaking early in the lek season. Interlek movements were positively associated with elevation. Seasonal interlek movements occurred more frequently than previously reported, and can bias lek counts in early spring as males move from low to high elevation leks, which reinforces interlek movements as a critical component of lek ecology. © 2016 The Wildlife Society.

Fremgen, Aleshia L.↗

The rapid quantitation of the filamentous blue-green alga plectonema boryanum by the luciferase assay for ATP

Plectonema boryanum is a filamentous blue green alga. Blue green algae have a procaryotic cellular organization similar to bacteria, but are usually obligate photoautotrophs, obtaining their carbon and energy from photosynthetic mechanism similar to higher plants. This research deals with a comparison of three methods of quantitating filamentous populations: microscopic cell counts, the luciferase assay for ATP and optical density measurements.

Bush, V. N.↗

Integrated Component-Specific Measurements to Develop Emission Factors for Compressors and Gas Gathering Lines

The Department of Energy (DOE) National Energy Technology Laboratory (NETL) and other critical stakeholders (e.g., U.S. Environmental Protection Agency (USEPA), state regulators, and industry) seek to more accurately characterize and quantify methane emissions from compressors and gathering lines within the Gathering and Boosting (G&B) segment of the natural gas system network. These efforts will reduce uncertainties in emissions estimates in USEPA’s current Green House Gas Inventory (GHGI) under their Greenhouse Gas Reporting Program (GHGRP). For this study, four G&B stations in the Gulf Coast Area were visited four times over the course of one year. Components were classified according to equipment and component type and emissions from select components were measured using multiple measurement technologies. The following major equipment types were considered: i) compressors, ii) separators, iii) dehydrators, iv) coalescers, v) slug catchers, vi) yard piping, vii) tanks, and viii) gathering lines. Individual components on each equipment type were further divided into the following main categories: i) connectors, ii) valves, iii) pressure relief valves (PRV), iv) meters, v) gauges, vi) regulators, vii) pneumatic device vents, and viii) compressor vents (e.g., distance piece vents [DPVs] and rod packing vents [RPVs]). Components were screened for the presence of leaks and venting emissions with an optical gas imaging (OGI) camera, and methane emission rates were measured using a microdilution high volume sampler. Downwind emissions were measured using an Open Path Fourier Transform Infrared (OP-FTIR) Spectrometer. Data were evaluated to assess correlation with site, equipment, time, and operational variables. Key findings and recommendations from the study include: A) Different counting methodologies can result in vastly different component counts, thus influencing total emissions calculations using Subpart W population EFs. A standard methodology should be adopted for classifying and counting components in order to minimize inconsistencies across studies and instruct stakeholders responsible for GHGI reporting. B) Results support current component subcategories listed in USEPA Subpart W, including the subdivision of connectors (flanged and “other”) and pneumatic device vents (intermittent, continuous low bleed, and continuous high bleed). C) Emission rates vary across sites and a relationship between higher emissions and compressor age was observed for some components. D) Component emission rates varied between field campaigns. The type of components contributing to overall measured emissions also varied among field campaigns. However, the emission rate variability was larger between different components (of the same category) than the variability in one component over the four field campaigns. Therefore, when calculating emission factors, it is not necessary to take repeat measurements to capture component variability. E) Measuring the emission rate of a sample for an extended period of time is not necessary to capture the average component emission rate. F) For reporting purposes, excluding liquid lines from site-wide screening may have a negligible effect on component-related emissions. However, from a site management perspective, malfunctioning components were not uncommon on liquid lines and contributed substantially to fugitive emissions at some sites. G) Population EFs, whether including or excluding liquid lines, were a conservative estimate of fugitive emissions. Leaker EFs consistently underestimated emissions at the field sites. Measurements at more sites, particularity sites in other areas of the country, need to be completed before determining the accuracy of EFs.

03 NATURAL GAS↗

Resolving the Extragalactic γ -Ray Background above 50 GeV with the Fermi Large Area Telescope

The Fermi Large Area Telescope (LAT) Collaboration has recently released a catalog of 360 sources detected above 50 GeV (2FHL). This catalog was obtained using 80 months of data re-processed with Pass 8, the newest event-level analysis, which significantly improves the acceptance and angular resolution of the instrument. Most of the 2FHL sources at high Galactic latitude are blazars. In this paper, using detailed Monte Carlo simulations, we measure, for the first time, the source count distribution, dN/dS, of extragalactic γ-ray sources at E > 50 GeV and find that it is compatible with a Euclidean distribution down to the lowest measured source flux in the 2FHL (~8 x 10 -12 ph cm -2 s -1 ). We employ a one-point photon fluctuation analysis to constrain the behavior of dN/dS below the source detection threshold. Overall, the source count distribution is constrained over three decades in flux and found compatible with a broken power law with a break flux, S b , in the range [8 x 10 -12 , 1.5 x 10 -11 ] ph cm -2 s -1 and power-law indices below and above the break of α 2 ϵ [1.60, 1.75] and α 1 = 2.49 ± 0.12, respectively. Integration of dN/dS shows that point sources account for at least 86$+16\atop{-14}$ % of the total extragalactic γ-ray background. The simple form of the derived source count distribution is consistent with a single population (i.e., blazars) dominating the source counts to the minimum flux explored by this analysis. Finally, we estimate the density of sources detectable in blind surveys that will be performed in the coming years by the Cherenkov Telescope Array.

79 ASTRONOMY AND ASTROPHYSICS↗

The NuSTAR extragalactic surveys: the number counts of active galactic nuclei and the resolved fraction of the cosmic X-ray background

We present the 3–8 keV and 8–24 keV number counts of active galactic nuclei (AGNs) identified in the Nuclear Spectroscopic Telescope Array (NuSTAR) extragalactic surveys. NuSTAR has now resolved 33%–39% of the X-ray background in the 8–24 keV band, directly identifying AGNs with obscuring columns up to ∼10{sup 25} cm{sup −2}. In the softer 3–8 keV band the number counts are in general agreement with those measured by XMM-Newton and Chandra over the flux range 5×10{sup −15} ≲ S(3–8 keV)/erg s{sup −1} cm{sup −2} ≲ 10{sup −12} probed by NuSTAR. In the hard 8–24 keV band NuSTAR probes fluxes over the range 2×10{sup −14} ≲ S(8–24 keV)/erg s{sup −1} cm{sup −2} ≲ 10{sup −12}, a factor ∼100 fainter than previous measurements. The 8–24 keV number counts match predictions from AGN population synthesis models, directly confirming the existence of a population of obscured and/or hard X-ray sources inferred from the shape of the integrated cosmic X-ray background. The measured NuSTAR counts lie significantly above simple extrapolation with a Euclidian slope to low flux of the Swift/BAT 15–55 keV number counts measured at higher fluxes (S(15–55 keV) ≳ 10{sup −11} erg s{sup −1} cm{sup −2}), reflecting the evolution of the AGN population between the Swift/BAT local (z<0.1) sample and NuSTAR’s z∼1 sample. CXB synthesis models, which account for AGN evolution, lie above the Swift/BAT measurements, suggesting that they do not fully capture the evolution of obscured AGNs at low redshifts.

79 ASTRONOMY AND ASTROPHYSICS↗

Salmonella Inactivation During Extrusion of an Oat Flour Model Food

Abstract Little research exists on Salmonella inactivation during extrusion processing, yet many outbreaks associated with low water activity foods since 2006 were linked to extruded foods. The aim of this research was to study Salmonella inactivation during extrusion of a model cereal product. Oat flour was inoculated with Salmonella enterica serovar Agona, an outbreak strain isolated from puffed cereals, and processed using a single‐screw extruder at a feed rate of 75 kg/h and a screw speed of 500 rpm. Extrudate samples were collected from the barrel outlet in sterile bags and immediately cooled in an ice–water bath. Populations were determined using standard plate count methods or a modified most probable number when populations were low. Reductions in population were determined and analyzed using a general linear model. The regression model obtained for the response surface tested was Log ( N R / N O ) = 20.50 + 0.82 T − 141.16a w – 0.0039 T 2 + 87.91 a w 2 ( R 2 = 0.69). The model showed significant ( p < 0.05) linear and quadratic effects of a w and temperature and enabled an assessment of critical control parameters. Reductions of 0.67 ± 0.14 to 7.34 ± 0.02 log CFU/g were observed over ranges of a w (0.72 to 0.96) and temperature (65 to 100 °C) tested. Processing conditions above 82 °C and 0.89 a w achieved on average greater than a 5‐log reduction of Salmonella . Results indicate that extrusion is an effective means for reducing Salmonella as most processes commonly employed to produce cereals and other low water activity foods exceed these parameters. Thus, contamination of an extruded food product would most likely occur postprocessing as a result of environmental contamination or through the addition of coatings and flavorings.

Anderson, Nathan M.↗

Effects of proton and gamma radiation on lymphocyte populations and acute response to antigen

BACKGROUND: The clinical use of proton radiation in the management of cancer, as well as benign disorders, is rapidly increasing. The major goal of this study was to compare the effects of proton and gamma (60Co) radiation on cell-mediated and humoral immunological parameters. MATERIALS AND METHODS: C57BL/6 mice were exposed to a single dose of 3 Gray (Gy) protons or gamma-rays and intraperitoneally injected 1 day later with sheep red blood cells (sRBC). On 4, 10, 15, and 29 days after exposure, subsets from each group were euthanised; nonirradiated controls (with and without sRBC injection) were included. Body and relative spleen weights, leukocyte counts, spontaneous blastogenesis, lymphocyte populations, and anti-sRBC titers were evaluated. RESULTS: The data showed significant depression (p < 0.05) in nearly all assays on days 4 and 10 after irradiation. B lymphocytes (CD19+) were the most radiosensitive, although reconstitution back to normal levels was observed by day 15. T cell (CD3+) and T helper cell (CD4+) recovery was evident by day 29, whereas the T cytotoxic cell (CD8+) count remained significantly below normal. Natural killer cells (NK1.1+) were relatively radioresistant. Anti-sRBC antibody production was slow and low titers were obtained after irradiation. No significant differences were noted between the two types of radiation. CONCLUSIONS: Taken together, the data show that whole-body irradiation with protons or gamma-rays, at the dose employed, results in marked, but transient, immunosuppression. However, at the time points of testing and with the assays used, little or no differences were found between the two forms of radiation.

NASA Discipline Radiation Health↗

The galactic spheroid

Analytic approximations and numerical simulations are used to derive the characteristic behavior of star counts in a galactic spheroidal population, whose visible stars' stellar luminosity function is obtainable for galactocentric distances between about 4 and 12 kpc from star count observations above 30 deg galactic latitude. The total densities of stars and of mass in the spheroid at the solar position are evaluated using different assumed luminosity functions, in order to extrapolate the measured values to a wider range of absolute magnitudes. The upper limits to the frequency of intermediate population stars are derived for the absolute visual magnitude range of 5-8. If such stars occur in either a flat disk with a scale height of 3 kpc or a spheroid with an ellipticity of 0.5, their local surface density is less than 1.8 times that of the spheroid.

Bahcall, J. N.↗