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At least 37 records · Page 2

Effect of outdoor exposure at ambient and elevated temperatures on fatigue life of Ti-6Al-4V titanium alloy sheet in the annealed and the solution treated and aged condition

Specimens of Ti-6Al-4V titanium alloy sheet in the annealed and the solution-treated and aged heat-treatment condition were exposed outdoors at ambient and 560 K (550 F) temperatures to determine the effect of outdoor exposure on fatigue life. Effects of exposure were determined by comparing fatigue lives of exposed specimens to those of unexpected specimens. Two procedures for fatigue testing the exposed specimens were evaluated: (1) fatigue tests conducted outdoors by applying 1200 load cycles per week until failure occurred and (2) conventional fatigue tests (continuous cycling until failure occurred) conducted indoors after outdoor exposure under static load. The exposure period ranged from 9 to 28 months for the outdoor fatigue-test group and was 24 months for the static-load group. All fatigue tests were constant-amplitude bending of specimens containing a drilled hole (stress concentration factor of 1.6). The results of the tests indicate that the fatigue lives of solution-treated and aged specimens were significantly reduced by the outdoor exposure at 560 K but not by the exposure at ambient temperature. Fatigue lives of the annealed specimens were essentially unaffected by the outdoor exposure at either temperature. The two test procedures - outdoor fatigue test and indoor fatigue test after outdoor exposure - led to the same conclusions about exposure effects.

Phillips, E. P.↗

On the outdoor thermal perception and comfort of a Mediterranean subject across other Koppen-Geiger's climate zones

Highlights: • Thermal perception for a Mediterranean normotype in the world. • Use of the Mediterranean Outdoor Comfort Index (MOCI). • Annual hourly distribution of the MOCI in the most visited cities of each continent. • Frequencies and percentiles of the average weekly thermal perception's values. • Mapping of the MOCI seasonal average values in the world. The climatic conditions characterizing the troposphere affect the outdoor activities of the population which, in turn, characterize the anthroposphere. Over the past years recent studies examined a possible analytical definition of the outdoor thermal comfort for different types of population acclimatized to their specific residency area. Thanks to the use of the MOCI (Mediterranean Outdoor Comfort Index: an index used to predict the thermal perception of the Mediterranean population in an outdoor environment), the present study aimed at analyzing the psychophysiological response of a Mediterranean subject with respect to climatic conditions different from the original ones after having experienced a quick change. Indeed, thermal stress and discomfort might affect deeply the ability of the subject to carry out outdoor activities. This condition determines the necessity of possessing useful information to plan the best period to perform a journey in distant places and/or perform useful actions that will make the outdoor activity as much comforting as possible (choosing the proper clothes with the right thermal insulation). For this reason, the worldwide environmental conditions during an average year were examined. To be more specific, this study focused its attention on the editing of seasonal spatial maps with the MOCI level curves and on the temporal analysis of the MOCI values characterizing those 6 different cities (one for each continent) reporting the higher number of foreign arrivals.

54 ENVIRONMENTAL SCIENCES↗

Evaluating indoor thermal resilience of passive and low-power cooling shelters for outdoor workers in India

India’s 231 million outdoor workers are exposed to deadly heat during heat waves. We report results from a simulation-based study that focuses on passive design of cooling shelters for the hot and dry climate zone of India. The goal is to design a cooler-than-outdoor shelter for outdoor workers’ intermittent rest and recovery from heat stress during their arduous outdoor work, to avoid heat-related morbidities and mortality. Expected indoor thermal conditions in low-cost cooling shelters deploying a range of passive designs and low-power active measures are investigated, consistent with the current Indian standards and practices, located in the city of Jodhpur in western India. Heat resilience of the shelter was measured with the predicted reduction of hazardous hours inside the shelter based on the wet-bulb globe temperature (WBGT) and extended heat index. These measures include improving the building envelope, installing cool roofs, using internal thermal mass, and natural ventilation. Additionally, a low-power active measure of using ventilation fans and ceiling fans is explored. EnergyPlus-based simulation results show that simple, commonly available measures, particularly natural ventilation and ceiling fans, achieve the most significant reductions in overheating danger hours, by 22% and 21%, respectively, while more complex or costly passive strategies yield only marginal additional benefits. Moreover, combined measure packages are identified that can reduce the indoor WBGT by 8 to 10 °C, eliminating overheating danger hours. These findings highlight the critical importance of scalable, low-cost, and easily deployable cooling solutions for developing resilient cooling shelters for vulnerable outdoor workers during heat waves in India.

building performance↗

Persistence of bacterial-mediated anti-rotifer protection in preliminary outdoor cultivation trial for Microchloropsis salina

Outdoor algal cultivation systems are susceptible to a wide variety of deleterious species. In previously published studies, we observed protection using microbial consortia at laboratory scale cultures; Microchloropsis salina in the presence of microbial consortia were protected from grazing from the marine rotifer, Brachionus plicatilis. Our objective for the present work was to determine if this protection conferred by microbial consortia in controlled laboratory experiments would persist in an open, outdoor multi-liter cultivation system. We found that algal protection did persist as evidenced by the presence of fewer motile rotifers and decreased rotifer-associated egg counts for the consortia-treated outdoor cultures. Due to the low temperature and light conditions that reduced growth of the algae outdoors, we performed an indoor laboratory assay which also confirmed the persistence of algal protection. Lastly, the lower numbers of motile rotifers and fewer rotifer-associated eggs in the consortia-treated algal cultures suggests a possible protective mechanism by the consortia through interfering with the rotifer lifecycle or reproduction. Finally, these initial results support the possibility that low cost, prophylactic treatments with microbial consortia can protect algae from deleterious species in outdoor cultivation systems.

59 BASIC BIOLOGICAL SCIENCES↗

Second-hand smoke exposure in outdoor hospitality venues: Smoking visibility and assessment of airborne markers

Highlights: • Smoking visibility remains widespread in outdoor hospitality venues in Madrid. • SHS levels are relatively high, especially in closed outdoor terraces. • Outdoor hospitality venues should be considered to become completely smoke-free. • Policymakers should ensure that the existing smoke-free regulations are enforced. After the implementation of smoke-free policies in indoor hospitality venues (including bars, cafeterias, restaurants, and pubs), smokers may have been displaced to their outdoor areas. We aimed to study smoking visibility and second-hand smoke exposure in outdoor hospitality venues.

54 ENVIRONMENTAL SCIENCES↗

Surveillance of Aedes aegypti indoors and outdoors using Autocidal Gravid Ovitraps in South Texas during local transmission of Zika virus, 2016 to 2018

The yellow fever mosquito, Aedes aegypti, has facilitated the re-emergence of dengue virus (DENV) and emergence of chikungunya virus (CHIKV) and Zika virus (ZIKV) in the Americas and the Caribbean. The recent transmission of these arboviruses in the continental United States has been limited, to date, to South Florida and South Texas despite Ae. aegypti occurring over a much larger geographical region within the country. The main goal of our study was to provide the first long term longitudinal study of Ae. aegypti and enhance the knowledge about the indoor and outdoor relative abundance of Ae. aegypti as a proxy for mosquito-human contact in South Texas, a region of the United States that is at high risk for mosquito-borne virus transmission. Here, the relative abundance of indoors and outdoors mosquitoes of households in eight different communities was described. Surveillance was done weekly from September 2016 to April 2018 using the CDC Autocidal Gravid Ovitraps in low- and middle-income communities. A total of 69 houses were included in this survey among which 36 were in the low-income communities (n = 11 for Donna, n = 15 for Progresso, n = 5 for Mesquite, n = 5 for Chapa) and 33 in middle-income communities (n = 9 for La Feria, n = 8 for Weslaco, n = 11 for McAllen, and n = 5 for Rio Rico). Overall, Ae. aegypti was the dominant species (59.2% of collections, n = 7255) followed by Culex spp. mosquitoes (27.3% of collections, n = 3350). Furthermore, we demonstrated for Ae. aegypti that 1) outdoor relative abundance was higher compared to indoor relative abundance, 2) low-income communities were associated with an increase in mosquito relative abundance indoors when compared to middle-income communities, 3) no difference was observed in the number of mosquitoes collected outdoors between low-income and middle-income communities, and 4) warmer months were positively correlated with outdoor relative abundance whereas no seasonality was observed in the relative abundance of mosquitoes indoors. Additionally, Ae. aegypti mosquitoes collected in South Texas were tested using a specific ZIKV/CHIKV multiplex real-time PCR assay, however, none of the mosquitoes tested positive. Our data highlights the occurrence of mosquitoes indoors in the continental United States and that adults are collected nearly every week of the calendar year. These mosquito data, obtained concurrently with local ZIKV transmission of 10 locally acquired cases in nearby communities, represent a baseline for future studies in the Lower Rio Grande Valley (LRGV) including vector control interventions relying on the oviposition behavior to reduce mosquito populations and pathogen transmission.

59 BASIC BIOLOGICAL SCIENCES↗

Indoor versus Outdoor Air Quality during Wildfires

The human behavioral modification recommendations during wildfire events are based on particulate matter and may be confounded by the potential risks of gas-phase pollutants such as polycyclic aromatic hydrocarbons (PAHs). Moreover, the majority of adults spend over 90 percent of their time indoors where there is an increased concern of indoor air quality during wildfire events. We address these timely concerns by evaluating paired indoor and outdoor PAH concentrations in residential locations and their relationship with satellite model-based categorization of wildfire smoke intensity. Low density polyethylene passive air samplers were deployed at six residential sites for one week in Eugene, Oregon with matched indoor and outdoor samples and twenty-four-hour time resolution. Samples were then quantitatively analyzed for 63 PAH concentrations using gas-chromatography. A probabilistic principal components analysis was used to reduce all 63 PAHs into an aggregate measure. Linear regression of the first principal component against indoor versus outdoor shows that indoor gas-phase PAH concentrations are consistently equal or greater than outdoor concentrations. Regression against a satellite-based model for wildfire smoke shows that outdoor, but not indoor gas-phase PAH concentrations are likely associated with wildfire events. Furthermore, these results point towards the need to include gas-phase pollutants such as PAHs in air pollution risk assessment.

54 ENVIRONMENTAL SCIENCES↗

Assessing Indoor versus Outdoor PM 2.5 Concentrations during the 2025 Los Angeles Fires Using the PurpleAir Sensor Network

In January 2025, a series of fast-moving wildland-urban-interface (WUI) fires swept through the Los Angeles (LA) metropolitan area, causing severe air pollution. While the impacts of WUI fires on outdoor air quality have been extensively studied, indoor exposure remains less understood, despite most people sheltering indoors during WUI fires. Here, this study investigates the spatial and temporal patterns of indoor and outdoor PM 2.5 concentrations across the South Coast Air Basin, with a focus on LA County during the LA fires. Using high-resolution data from co-located indoor and outdoor PurpleAir (PA) sensors, we analyze hourly PM 2.5 levels and indoor/outdoor ratios. Outdoor PM 2.5 concentrations spiked sharply during the fires, reaching unhealthy levels exceeding 130 μg/m 3 , compared to the mean concentration (12 μg/m 3 ) during non-fire hours. Indoor concentrations also increased, though to a lesser extent, peaking around 60 μg/m 3 compared to a mean of 7 μg/m 3 during non-fire hours. This reflects the partial shielding that indoor environments provide from outdoor air pollution. The mean (0.42) and median (0.29) indoor/outdoor PM 2.5 ratios during LA fire hours were lower than the mean (0.93) and median (0.66) ratios during non-fire hours. Indoor/outdoor PM 2.5 ratios across sensors showed a wide distribution, reflecting differences in building characteristics and occupant behavior, such as indoor activities and the use of air purifiers. These findings emphasize the need for guidance and interventions to reduce indoor PM 2.5 exposure and protect public health during extreme WUI fire events.

2025 Los Angeles Fires↗

Comparing Outdoor to Indoor Performance for Bifacial Modules Affected by Polarization-Type Potential-Induced Degradation

Bifacial photovoltaic (PV) modules have the advantage of using light reflected off of the ground to contribute to power production. Predicting the energy gain is challenging and requires complex models to do so accurately. Often, module degradation over time is neglected in models for the sake of simplicity or is underestimated. Comparing outdoor and indoor current–voltage (I–V) performance for bifacial modules is more challenging than for monofacial modules, as there are additional variables to consider such as rear albedo non-uniformity, cell mismatch, and their effects on temperature. This challenge is compounded when heterogeneous degradation modes occur, such as polarization-type potential-induced degradation (PID-p). To examine the effects of PID-p on I–V predictions using an empirical data-driven approach, 16 bifacial PERC modules are installed outdoors on racks with different albedo conditions. A subset is exposed to high-voltage biases of −1500 V or +1500 V. Outdoor data are traced at irradiance ranges of 150–250 W/m 2 , 500–600 W/m 2 , and 900–1000 W/m 2 . These curves are corrected using control module temperature, wire resistivity, and module resistance measured indoors. We examine several methods to transform indoor I–V curves to accurately, and more simply than existing methods, approximate outdoor performance for bifacial modules without and with varying levels of PID-p degradation. This way, bifacial performance modeling can be more accessible and informed by fielded, degraded modules. Distributions of percent errors between indoor and outdoor performance parameters and Mean Absolute Percent Errors (MAPEs) are used to assess method quality. Results including low-irradiance data (150–250 W/m 2 ) are discussed but are filtered for quantifying method quality as these data introduce substantial errors. The method with the most optimal tradeoff between low MAPE and analysis simplicity involves measuring the front side of a module indoors at an irradiance equal to plane-of-array irradiance plus the product of module bifaciality and albedo irradiance. This method gives MAPE values of 1–6.5% for non-degraded and 1.6–5.9% for PID-p degraded module performance.

14 SOLAR ENERGY↗

Loudness and annoyance response to simulated outdoor and indoor sonic booms

The sonic boom simulator of the Langley Research Center was used to quantify subjective loudness and annoyance response to simulated indoor and outdoor sonic boom signatures. The indoor signatures were derived from the outdoor signatures by application of house filters that approximated the noise reduction characteristics of a residential structure. Two indoor listening situations were simulated: one with the windows open and the other with the windows closed. Results were used to assess loudness and annoyance as sonic boom criterion measures and to evaluate several metrics as estimators of loudness and annoyance. The findings indicated that loudness and annoyance were equivalent criterion measures for outdoor booms but not for indoor booms. Annoyance scores for indoor booms were significantly higher than indoor loudness scores. Thus, annoyance was recommended as the criterion measure of choice for general use in assessing sonic boom subjective effects. Perceived level was determined to be the best estimator of annoyance for both indoor and outdoor booms, and of loudness for outdoor booms. It was recommended as the metric of choice for predicting sonic boom subjective effects.

Leatherwood, Jack D.↗

Growth reponses of eggplant and soybean seedlings to mechanical stress in greenhouse and outdoor environments

Eggplant (Solanum melongena L. var. esculentum 'Burpee's Black Beauty') and soybean [Glycine max (L.) Merr. 'Wells II'] seedlings were assigned to a greenhouse or a windless or windy outdoor environment. Plants within each environment received either periodic seismic (shaking) or thigmic (flexing or rubbing) treatment, or were left undisturbed. Productivity (dry weight) and dimensional (leaf area and stem length) growth parameters generally were reduced more by mechanical stress in the greenhouse (soybean) or outdoor-windless environment (eggplant) than in the outdoor windy environment. Outdoor exposure enhanced both stem and leaf specific weights, whereas mechanical stress enhanced only leaf specific weight. Although both forms of controlled mechanical stress tended to reduce node and internode diameters of soybean, outdoor exposure increased stem diameter.

NASA Discipline Number 61-10↗

Localized real-time information on outdoor air quality at kindergartens in Oslo, Norway using low-cost sensor nodes

Highlights: • We show that low-cost sensors can provide an indication of outdoor air quality. • We employed a data fusion method to provide real-time air quality maps. • Localized real-time air quality information can help parents and kindergarten staff. In Norway, children in kindergartens spend significant time outdoors under all weather conditions, and there is thus a natural concern about the quality of outdoor air. It is well known that air pollution is associated with a wide variety of adverse health impacts for children, with greater impact on children with asthma. Especially during winter and spring, kindergartens in Oslo that are situated close to streets with busy traffic, or in areas where wood burning is used for house heating, can experience many days with bad air quality. During these periods, updated information on air quality levels can help the kindergarten teachers to plan appropriate outdoor activities and thus protect children's health. We have installed 17 low-cost air quality nodes in kindergartens in Oslo. These nodes are smaller, cheaper and less complex to use than traditional equipment. Performance evaluation shows that while they are less accurate and suffer from higher uncertainty than reference equipment, they still can provide reliable coarse information about local pollution. The main challenge when using this technology is that calibration parameters might change with time depending on the atmospheric conditions. Thus, even if the sensors are calibrated a priori, once deployed, and especially if they are deployed for a long time, it is not possible to determine if a node is over- or under-estimating the concentration levels. To enhance the data from the sensors, we employed a data fusion technique that allows generating a detailed air quality map merging the data from the sensors and the data from an urban model, thus being able to offer air quality information to any location within Oslo. We arranged a focus group with the participation of local administration, kindergarten staff and parents to understand their opinion and needs related to the air quality information that was provided to the participant kindergartens. They expressed concern about the data quality but agree that having updated information on the air quality in the surroundings of kindergartens can help them to reduce children's exposure to air pollution.

54 ENVIRONMENTAL SCIENCES↗

Glass-particle adhesion-force-distribution on clean (laboratory) and contaminated (outdoor) surfaces

This article explores whether laboratory-clean samples are representative of real outdoor surfaces – often covered in dust and other contaminants – with respect to adhesion properties, which ultimately affect particle resuspension. In this study, Atomic Force Microscopy (AFM) was used to measure the adhesion force between a clean, smooth glass particle (5 µm in diameter) and two glass slides, one prepared according to common laboratory practices (hereafter, clean glass) and a second one left outdoors for six weeks (hereafter, contaminated glass). It was found that the adhesion force distribution was bimodal for the clean glass while it showed three peaks for the contaminated glass. For both surfaces, the adhesion force showed a peak at very low adhesion values (< 100 nN) representing 1.2% of the measurements for the clean glass and 58% for the contaminated glass. This low-adhesion peak is important because it represents the particles that would be resuspended first even in light winds. The low adhesion-force measurements were associated with the presence of contaminants on the surface. Another notable difference between the adhesion force distributions was a long tail at high adhesion forces for clean glass (up to ~1900 nN) and the absence of the tail for the contaminated glass, which had a maximum adhesion force of 950 nN. The mean adhesion force on the contaminated glass was also lower than on the clean glass (206 ± 240 nN vs. 516 ± 354 nN). These observations indicate that the adhesion force distribution between a 5-µm smooth and clean glass sphere and the outdoor contaminated glass surface is quite different from that of the clean glass surface. Hence, one should be cautious when using adhesion force measurements from clean surfaces to estimate particle resuspension from outdoor surfaces.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

Absolute Cavity Pyrgeometer to Measure the Absolute Outdoor Longwave Irradiance with Traceability to International System of Units, SI

This article describes a method of measuring the absolute outdoor longwave irradiance using an absolute cavity pyrgeometer (ACP), U.S. Patent application no. 13/049, 275. The ACP consists of domeless thermopile pyrgeometer, gold-plated concentrator, temperature controller, and data acquisition. The dome was removed from the pyrgeometer to remove errors associated with dome transmittance and the dome correction factor. To avoid thermal convection and wind effect errors resulting from using a domeless thermopile, the gold-plated concentrator was placed above the thermopile. The concentrator is a dual compound parabolic concentrator (CPC) with 180{sup o} view angle to measure the outdoor incoming longwave irradiance from the atmosphere. The incoming irradiance is reflected from the specular gold surface of the CPC and concentrated on the 11 mm diameter of the pyrgeometer's blackened thermopile. The CPC's interior surface design and the resulting cavitation result in a throughput value that was characterized by the National Institute of Standards and Technology. The ACP was installed horizontally outdoor on an aluminum plate connected to the temperature controller to control the pyrgeometer's case temperature. The responsivity of the pyrgeometer's thermopile detector was determined by lowering the case temperature and calculating the rate of change of the thermopile output voltage versus the changing net irradiance. The responsivity is then used to calculate the absolute atmospheric longwave irradiance with an uncertainty estimate (U{sub 95}) of {+-}3.96 W m{sup 02} with traceability to the International System of Units, SI. The measured irradiance was compared with the irradiance measured by two pyrgeometers calibrated by the World Radiation Center with traceability to the Interim World Infrared Standard Group, WISG. A total of 408 readings were collected over three different nights. The calculated irradiance measured by the ACP was 1.5 W/m{sup 2} lower than that measured by the two pyrgeometers that are traceable to WISG, with a standard deviation of {+-}0.7 W m{sup -2}. These results suggest that the ACP design might be used for addressing the need to improve the international reference for broadband outdoor longwave irradiance measurements.

14 SOLAR ENERGY↗

Seasonal Impact of Regional Outdoor Biomass Burning on Air Pollution in Three Indian Cities: Delhi, Bengaluru, and Pune

Air pollution in many of India's cities exceeds national and international standards, and effective pollution control strategies require knowledge of the sources that contribute to air pollution and their spatiotemporal variability. In this study, we examine the influence of a single pollution source, outdoor biomass burning, on particulate matter (PM) concentrations, surface visibility, and aerosol optical depth (AOD) from 2007 to 2013 in three of the most populous Indian cities. We define the upwind regions, or "airsheds," for the cities by using atmospheric back trajectories from the HYSPLIT model. Using satellite fire radiative power (FRP) observations as a measure of fire activity, we target pre-monsoon and post-monsoon fires upwind of the Delhi National Capital Region and pre-monsoon fires surrounding Bengaluru and Pune. We find varying contributions of outdoor fires to different air quality metrics. For the post-monsoon burning season, we find that a subset of local meteorological variables (air temperature, humidity, sea level pressure, wind speed and direction) and FRP as the only pollution source explained 39% of variance in Delhi station PM(sub 10) anomalies, 77% in visibility, and 30% in satellite AOD; additionally, per unit increase in FRP within the daily airshed (1000 MW), PM(sub 10) increases by 16.34 micrograms per cubic meter, visibility decreases by 0.097 km, and satellite AOD increases by 0.07. In contrast, for the pre-monsoon burning season, we find less significant contributions from FRP to air quality in all three cities. Further, we attribute 99% of FRP from post-monsoon outdoor fires within Delhi's average airshed to agricultural burning. Our work suggests that although outdoor fires are not the dominant air pollution source in India throughout the year, post-monsoon fires contribute substantially to regional air pollution and high levels of population exposure around Delhi. During 3-day blocks of extreme PM(sub 2.5) in the 2013 post-monsoon burning season, which coincided with statistically significant high fire activity, concentrations in Delhi averaged 304 micrograms per cubic meter, or more than 1000% above the 24-h PM(sub 2.5) guideline (25 micrograms per cubic meter) of the World Health Organization. These results suggest that providing viable alternatives to agricultural residue burning could help improve post-monsoon air quality for a growing population of 63 million (39% in urban areas) within Delhi's airshed.

Air quality; Outdoor fires; Crop residue burning; ↗

Spectrally Selective Nanocomposite Textile for Outdoor Personal Cooling

Outdoor heat stress poses a serious public health threat and curtails industrial labor supply and productivity, thus adversely impacting the wellness and economy of the entire society. With climate change, there will be more intense and frequent heat waves that further present a grand challenge for sustainability. However, an efficient and economical method that can provide localized outdoor cooling of the human body without intensive energy input is lacking. Here, a novel spectrally selective nanocomposite textile for radiative outdoor cooling using zinc oxide nanoparticle–embedded polyethylene is demonstrated. By reflecting more than 90% solar irradiance and selectively transmitting out human body thermal radiation, this textile can enable simulated skin to avoid overheating by 5–13 °C compared to normal textile like cotton under peak daylight condition. In conclusion, owing to its superior passive cooling capability and compatibility with large–scale production, this radiative outdoor cooling textile is promising to widely benefit the sustainability of society in many aspects spanning from health to economy.

36 MATERIALS SCIENCE↗

Oxygen stress mitigation for microalgal biomass productivity improvement in outdoor raceway ponds

Dissolved oxygen (DO) concentrations at supersaturated levels are commonly observed in outdoor microalgae cultures due to photosynthesis during periods of rapid growth. Paradoxically, the photosynthetically generated oxygen is inhibitory and lowers biomass productivity, therefore, increasing the cost of microalgal biofuels. In this study, photosynthetic rates of Monoraphidium minutum 26B-AM and Scenedesmus obliquus UTEX393, two of the top DISCOVR strains, were compared at three DO levels, 0, 8, and 30 mg L -1 . Increasing DO from 0 to 30 mg L -1 caused a 36.6% and 26.1% reduction in photosynthetic rate for M. minutum 26B-AM and S. obliquus UTEX393, respectively. To improve the outdoor pond biomass productivity of each strain, three oxygen stress mitigation methods were tested. The first method raised the dissolved bicarbonate concentration by an order of magnitude to favor bicarbonate transport into the cell, increasing the CO 2 :O 2 ratio near RuBisCO, thus reducing photorespiration. Although this method increased the observed photosynthesis rate of M. minutum 26B-AM by 26% and S. obliquus UTEX393 by 13% in laboratory testing, it failed to improve biomass productivity under outdoor pond conditions for both strains. The second method added sodium sulfite as an oxygen scavenger to chemically lower the DO concentration in the culture. Chemical reduction of dissolved molecular oxygen did not significantly improve the biomass productivity of M. minutum 26B-AM; however, a 10% improvement in biomass productivity for S. obliquus UTEX393 was observed. The final method sparged cultures with air to strip oxygen out of the liquid medium. This did not improve the biomass productivity of M. minutum 26B-AM, likely due to unfavorable weather conditions during cold season cultivation; however, an improvement of 36% in biomass productivity was achieved for S. obliquus UTEX393 during the warm season cultivation. Of the three tested methods, air-sparging was the most effective and practical for improving outdoor biomass production.

59 BASIC BIOLOGICAL SCIENCES↗

Summer outdoor temperature and occupational heat-related illnesses in Quebec (Canada)

Background: Predicted rise in global mean temperature and intensification of heat waves associated with climate change present an increasing challenge for occupational health and safety. Although important scientific knowledge has been gathered on the health effects of heat, very few studies have focused on quantifying the association between outdoor heat and mortality or morbidity among workers. Objective: To quantify the association between occupational heat-related illnesses and exposure to summer outdoor temperatures. Methods: We modeled 259 heat-related illnesses compensated by the Workers' Compensation Board of Quebec between May and September, from 1998 to 2010, with maximum daily summer outdoor temperatures in 16 health regions of Quebec (Canada) using generalized linear models with negative binomial distributions, and estimated the pooled effect sizes for all regions combined, by sex and age groups, and for different time lags with random-effect models for meta-analyses. Results: The mean daily compensation count was 0.13 for all regions of Quebec combined. The relationship between daily counts of compensations and maximum daily temperatures was log-linear; the pooled incidence rate ratio (IRR) of daily heat-related compensations per 1 °C increase in daily maximum temperatures was 1.419 (95% CI 1.326 to 1.520). Associations were similar for men and women and by age groups. Increases in daily maximum temperatures at lags 1 and 2 and for two and three-day lag averages were also associated with increases in daily counts of compensations (IRRs of 1.206 to 1.471 for every 1 °C increase in temperature). Conclusion: This study is the first to quantify the association between occupational heat-related illnesses and exposure to summer temperatures in Canada. The model (risk function) developed in this study could be useful to improve the assessment of future impacts of predicted summer outdoor temperatures on workers and vulnerable groups, particularly in colder temperate zones. - Highlights: • 259 heat-related compensated illnesses were modeled with ambient temperature • An overall risk ratio of 1.419 (95% CI 1.326–1.520) for every 1 °C increase was found • Risk estimates were similar for men and women and by large age groups. • There were little lag effects (IRRs of 1.206 to 1.471 for every 1 °C increase)

54 ENVIRONMENTAL SCIENCES↗