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Beyond Low-Earth Orbit: Characterizing Immune and microRNA Differentials Following Simulated Deep Spaceflight Conditions in Mice

Spaceflight missions can cause immune system dysfunction in astronauts with little understanding of immune outcomes in deep space. This study assessed immune responses in mice following ground-based, simulated deep spaceflight conditions, compared to data from astronauts on ISS missions. For ground studies, we simulated microgravity using the hindlimb unloaded mouse model alone or in combination with acute simulated galactic cosmic rays or solar particle events irradiation. Immune profiling results revealed unique immune diversity following each experimental condition, suggesting each stressor results in distinct circulating immune responses, with clear consequences for deep spaceflight. Circulating plasma microRNA sequence analysis revealed involvement in immune system dysregulation. Furthermore, a large astronaut cohort showed elevated inflammation during LEO missions, thereby supporting our simulated ground experiments in mice. Herein, circulating immune biomarkers are defined by distinct deep space irradiation types coupled to simulated microgravity and could be targets for future space health initiatives.

deep spaceflight stressors↗

Production of simple molecules on the surface of Mercury

Lunar sample studies have shown that solar-wind irradiation of the lunar surface has produced a variety of low-molecular-weight compounds. Analysis of the lunar soils has revealed the presence of H2, CH4, H2O, N2, CO, CO2, He, Ne and other components which are extralunar. Irradiation experiments on lunar materials and analogs have shown that solar-wind and solar-flare irradiation of the lunar surface produces selected low-molecular-weight components. Solar-wind irradiation of Mercury's surface should also produce a wide variety of low-molecular-weight species because of the increased solar flux, which results from Mercury being nearer the sun than the moon. The thermal regime of Mercury's surface would result in thermal evaporation of low-temperature components followed by 'cold-trapping' on the night-time side of the planet. Such desorption-adsorption processes assist chemical weathering of Mercury's regolith.

Gibson, E. K., Jr.↗

Chemistry of the CO dimer at low temperatures

Researchers conducted a series of experiments on the chlorine-catalyzed photodecomposition of O sub 3 both in the gas and in inert solvents such as CF sub 4 and CO sub 2 in the temperature range about 190 to 225 K. The liquid medium was chosen in order to minimize possible surface loss of long-lived ClO dimer, and to aid in the stabilization of transient excited intermediates. The mechanism of dimer formation was as follows: (1) Cl sub 2 + hv yields Cl + Cl; (2) Cl + O sub 3 yields ClO + O sub 2; (3) ClO + ClO yields Cl sub 2 O sub 2. The experiments were done in cooled low temperature cells, with irradiation from an Osram high pressure mercury arc, filtered to remove radiation below 325 nm. Spectral analysis was by means of a Cary Model 2200 UV spectrometer. The principal objectives were: (1) to determine the lifetime of the dimer as a function of temperature; (2) to observe spectral changes in the mixtures which could be attributed to dimer or related products; and (3) to observe chemical or photochemical reactions of the dimer.

Demore, W. B.↗

Dome Degradation Characterization of Wide-Field-of-View Nonscanner Aboard ERBE and Its Reprocessing

Earth Radiation Budget Experiment (ERBE) wide-field-of-view (WFOV) nonscanners aboard ERBS and NOAA- 9/NOAA-10 provided broadband shortwave and longwave irradiances from 1985 to 1999. The previous analysis showed dome degradation in the shortwave nonscanner instruments. The correction was performed with a constant spectral (gray assumption) degradation. We suspect that the gray assumption affected daytime longwave irradiance and led to a day-minus-night longwave flux differences (little change in night time longwave) increase over time. Based on knowledge from the CERES process, we will reprocess entire ERBE nonscanner radiation dataset by characterizing shortwave dome transmissivity with spectral dependent degradation using the solar data observed by these instruments. Once spectral dependent degradation is derived, imager derived cloud fraction and the cloud phase as well as surface type over the FOV of nonscanner instruments will be used to model unfiltering coefficients. This poster primarily explains the reprocessing techniques and includes initial comparison of several months of data processed with existing and our recent methods.

Shrestha, Alok K.↗

GeneLab: A Systems Biology Platform for Omics Analysis

NASA GeneLab is an open-access repository for omics datasets generated by biological experiments conducted in space or experiments relevant to spaceflight (e.g. simulated cosmic radiation, simulated microgravity, bed rest studies). The GeneLab Data Systems (GLDS) version 4.0 will be available on October 1st 2019, and will provide the latest in terms of professional state-of-the-art bioinformatics platform for the space biology and radiation community to upload their data into an omics data commons, to process their data with vetted standard workflows and to compare to existing analyses. Started in 2015 as a repository designed to archive omics data from space experiments, GeneLab has expanded its scope to all ionizing radiation omics experiments conducted on the ground and has put considerable effort in providing carefully characterized radiation metadata on all dataset. GeneLab is also providing processed data derived from the raw data covering a large spectrum of omics (genome, epigenome, transcriptome, epitranscriptome, proteome, metabolome) to help users explore important questions: 1) Which genes or proteins are expressed differently in space for various living organisms? 2) What specific DNA mutations or epigenetic changes happen in space or after exposure to ionizing radiation? and 3) How does genetics affect these responses? Processed data available on GeneLab are derived by standard data analysis workflows vetted by hundreds of scientists who volunteered to join one of the four GeneLab Analysis Working Groups (Animal AWG, Plant AWG, Microbe AWG, Multi-Omics AWG). In this presentation, we will discuss how to bridge the gap between irradiation studies performed on earth and biological experiments conducted in space since the early 1990's. We will discuss how radiation dosimetry was estimated for datasets derived from samples collected during the Space Shuttle era or on the International Space Station. Finally, we will address future strategies regarding dose monitoring in future missions into space, inter-agency efforts to unify data under one umbrella, and knowledge dissemination across the radiation research community and the space biology community.

open-science↗

NASA GeneLab Space Omics Database: Expanding from Space to Ionizing Radiation Data on the Ground

NASA GeneLab is an open-access repository for omics datasets generated by biological experiments conducted in space or ground experiments relevant to spaceflight (e.g. simulated cosmic radiation, simulated microgravity, bed rest studies). The GeneLab Data Systems (GLDS) version 4.0 will be available on October 1st 2019, and will provide a state-of-the-art bioinformatics platform for the space biology and radiation communities to upload their data into an omics data commons, to process their data with vetted standard workflows and to compare with existing analyses. Started in 2015 as a repository designed to archive omics data from space experiments, GeneLab has expanded its scope to all ionizing radiation omics experiments conducted on the ground and has put considerable effort in providing carefully characterized radiation metadata on all datasets. GeneLab is also providing processed data derived from the raw data covering a large spectrum of omics (genome, epigenome, transcriptome, epitranscriptome, proteome, metabolome) to help users explore important questions: 1) Which genes or proteins are expressed differently in space for various living organisms? 2) What specific DNA mutations or epigenetic changes happen in space or after exposure to ionizing radiation? and 3) How does genetics affect these responses? Processed data available on GeneLab are derived by standard data analysis workflows vetted by hundreds of scientists who volunteered to join one of the four GeneLab Analysis Working Groups (Animal AWG, Plant AWG, Microbe AWG, Multi-Omics AWG). In this presentation, we will discuss how to bridge the gap between irradiation studies performed on earth and biological experiments conducted in space since the early 1990's. We will discuss how radiation dosimetry was estimated for datasets derived from samples collected during the Space Shuttle era on the International Space Station and on other orbiting platforms. Finally, we will address future strategies regarding dose monitoring in future missions into space, inter-agency efforts to unify data under one umbrella, and knowledge dissemination across the radiation research community and the space biology community.

open-science↗

Proton irradiation of simple gas mixtures: Influence of irradiation parameters

In order to get information about the influence of irradiation parameters on radiolysis processes of astrophysical interest, methane gas targets were irradiated with 6.5 MeV protons at a pressure of 1 bar and room temperature. Yields of higher hydrocarbons like ethane or propane were found by analysis of irradiated gas samples using gas chromatography. The handling of the proton beam was of great experimental importance for determining the irradiation parameters. In a series of experiments current density of the proton beam and total absorbed energy were shown to have a large influence on the yields of produced hydrocarbons. Mechanistic interpretations of the results are given and conclusions are drawn with regard to the chemistry and the simulation of various astrophysical systems.

Sack, Norbert J.↗

New radiometric techniques and solar constant measurements.

A series of absolute cavity radiometers, designed to measure solar irradiance, has been developed at the Jet Propulsion Laboratory. Analysis indicates the absolute uncertainty of irradiance measurements made by the most recent devices to be less than plus or minus 0.5 mW/sq cm. In a series of experiments the radiometric scale defined by the JPL instruments has been compared with the International Pyrheliometric Scale (IPS). A systematic 2.2% difference has been observed with the IPS producing consistently lower measurements. The solar constant and spectral distribution have been derived from high-altitude balloon flight measurements made by two types of JPL cavity radiometers. Measurements at 25 km in 1968 produced a solar constant value of 137.0 mW/sq cm. From 1969 measurements at 36 km, a value of 136.6 mW/sq cm was derived, with an estimated absolute uncertainty of plus or minus 0.5%. The solar spectrum information from these experiments agrees most closely with the solar spectrum model of Labs and Neckel (1968).

Willson, R. C.↗

Inorganic chemical investigation by X-ray fluorescence analysis - The Viking Mars Lander

The inorganic chemical investigation experiment added in August 1972 to the Viking Lander scientific package uses an energy-dispersive X-ray fluorescence spectrometer in which four sealed, gas-filled proportional counters detect X-rays emitted from samples of the Martian surface materials irradiated by X-rays from radioisotope sources (Fe-55 and Cd-109). The instrument is inside the Lander body, and samples are to be delivered to it by the Viking Lander Surface Sampler. Instrument design is described along with details of the data processing and analysis procedures. The results of the investigation will characterize the surface materials of Mars as to elemental composition with accuracies ranging from a few tens of parts per million (at the trace-element level) to a few per cent (for major elements) depending on the element in question.

Toulmin, P., III↗

Simulation of Radiation-Induced DNA Damage With the Code RITRACKS

INTRODUCTION DNA damage is one of the most physiologically important effects of ionizing radiation. Clustered DNA damage events, like double-strand breaks (DSBs), have the most notable biological consequences. DNA damage types depend on both the track structure of the radiation and the spatial organization of the DNA. High linear energy transfer (LET) charged nuclei, found in galactic cosmic rays (GCR), are known to produce large numbers of complex DNA damage events. The human genome is packaged into chromatin, which can take on locus-dependent and cell type-dependent spatial conformations that correspond to epigenetic states, such as more open, extended structures in transcriptionally active chromatin. These epigenetic differences can affect DNA break patterns in response to ionizing radiation, potentially creating distinct DNA repair and signaling outcomes across the genome in different cells. MATERIAL AND METHODS The code RITRACKS (Relativistic Ion Tracks), which simulates stochastic radiation track structures and radiation chemistry, was used to model damage on isolated and histone-bound DNA by various types of ions and photons. The changes made to the code to perform radiation-induced DNA damage, and simulation results on single nucleosomes are given in our recent paper. In this work, the DNA building capabilities of RITRACKS have been extended to simulate more complex DNA structures build on the coarse-grain simulation framework meso-WLCsim. This code can sample generic chromatin fiber conformation ensembles based on the geometry of nucleosomes and mechanical properties of DNA. Using RITRACKS, we simulated the fragment length distributions (FLD) of irradiated DNA structures built using the chromatin conformations of WLCsim and obtained results representative of those obtained with Radiation-Induced Correlated Cleavage with sequencing (RICC-Seq) experiments [6]. We have also performed Fe ion and photon irradiations of K562, IMR90, BJ and RPE-1 cells at NSRL to experimentally validate results. Sample processing and data analysis are in progress and any available preliminary results will be discussed. DISCUSSION The recent updates in the code RITRACKS allow the calculation of several quantities such as the DNA damage yield and the FLD. This approach can be used to model epigenetic state-specific chromatin structure parameters to leverage the epigenetic state data available for many human cell types to infer relative DNA damage sensitivity among genomic loci.

I Plante↗

Precompaction irradiation effects: Particles from an early active sun?

Two recent studies have shown that solar flare irradiated grains from Murchison and Kapoeta have excess spallogenic Ne-21 compared to unirradiated grains, indicating large precompaction particle irradiation effects. The quantity of cosmogenic neon in these grains presents serious difficulties for either galactic cosmic ray or normal solar flare sources. In the first study it was suggested that the effect might be the result of exposure to an early active sun. The more recent experiment both confirms the earlier results and provides constraints on the characteristic energy spectrum on the irradiation. The first results were obtained from Murchison olivines and Kapoeta pyroxenes by mass spectrometric analysis of sets of grains selected on the basis of the presence or absence of solar flare particle tracks. In the second work plagioclase feldspar grains from Kapoeta were studied.

Caffee, M. W.↗

Guidelines for the Selection of Near-Earth Thermal Environment Parameters for Spacecraft Design

Thermal analysis and design of Earth orbiting systems requires specification of three environmental thermal parameters: the direct solar irradiance, Earth's local albedo, and outgoing longwave radiance (OLR). In the early 1990s data sets from the Earth Radiation Budget Experiment were analyzed on behalf of the Space Station Program to provide an accurate description of these parameters as a function of averaging time along the orbital path. This information, documented in SSP 30425 and, in more generic form in NASA/TM-4527, enabled the specification of the proper thermal parameters for systems of various thermal response time constants. However, working with the engineering community and SSP-30425 and TM-4527 products over a number of years revealed difficulties in interpretation and application of this material. For this reason it was decided to develop this guidelines document to help resolve these issues of practical application. In the process, the data were extensively reprocessed and a new computer code, the Simple Thermal Environment Model (STEM) was developed to simplify the process of selecting the parameters for input into extreme hot and cold thermal analyses and design specifications. In the process, greatly improved values for the cold case OLR values for high inclination orbits were derived. Thermal parameters for satellites in low, medium, and high inclination low-Earth orbit and with various system thermal time constraints are recommended for analysis of extreme hot and cold conditions. Practical information as to the interpretation and application of the information and an introduction to the STEM are included. Complete documentation for STEM is found in the user's manual, in preparation.

Anderson, B. J.↗

Selective photodestruction of alpha-amino acids

A problem encountered in the analysis of amino acids in chemical evolution experiments and in extracts of meteorites is the large number present. A method for selectively destroying the alpha-amino acids, with only the beta- and gamma-amino acids remaining in the solution, is described. The amino acids used were racemic, with one milliliter of solution containing 0.0000025 mol of each acid irradiated in a 1-cm quartz cell having 254-nm monochromatic light in the presence of CuCl2. Excess H2S was added to precipitate the Cu (2+) as CuS. A gas chromatographic analysis was used to observe that irradiation with 254-nm light in the presence of Cu (2+) destroyed all the amino acids except the beta and the gamma types. It is concluded that with such a procedure, complex mixtures of amino acids can be simplified to make identification by GC mass spectrometry easier.

Levi, N.↗

In-situ Measurements of the Radiolytic Destruction of Glycine in Ices: Applications to the Martian Subsurface

Amino acids and other organic molecules are thought to be easily destroyed on the surface of Mars by the high flux of incident ultraviolet rays or by chemical interactions with oxidizing substances in the soil. However, organic molecules may survive in the subsurface, where chemical processes are driven by penetrating galactic cosmic rays such as MeV protons. Models of the radiation dose as a function of depth on Mars have shown that the contribution of galactic cosmic rays dominates from about one centimeter to a few meters [1 J. Theoretical models have also been published to aid in understanding molecular destruction at these depths, but these usually are based on room-temperature laboratory data, studies of single-component samples, and ex-situ methods of chemical analysis. Recent studies of amino-acid survivability include those involving UV photolysis [2, 3J and gamma radiolysis [4], but nearly all chemical and kinetic analyses from such experiments involved room-temperature measurements on samples irradiated and then removed from sealed containers. We report new laboratory studies of the radiation-induced destruction of glycine-containing ices. In-situ infrared spectroscopy was used to study decay rates as a function of temperature and initial glycine concentrations. Our results indicate that glycine's destruction rate depends on temperature, the presence of H20-ice, and the initial relative abundance of glycine. These trends are not obvious in previous work, suggesting that room-temperature measurements on pure glycine's radiation stability are not directly applicable to Mars and other environments.

Gerakines, Perry A.↗

2022 Power Global Community (Gloco) Summit Summary Report

The Prediction Of Worldwide Energy Resources (POWER) Project 25th year of providing global solar and meteorology data products to the world. The goals of this event were to inform users of new, enhanced features, gather feedback on services, identify new requests and requirements, and capture more thorough user stories while identifying new user communities and partnership opportunities. The event was “virtual” and had over 580 registrants. Over the two-day event, the Summit was attended by 162 unique attendees representing 24 countries.

NASA↗

Space Weathering of Silicates Simulated by Successive Laser Irradiation: in Situ Reflectance Measurements of Fo90, Fo99+, and SiO2

Pulsed-laser irradiation causes the visible-near-infrared spectral slope of olivine (Fo90 and Fo99+) and SiO2 to increase (redden), while the olivine samples darken and the SiO2 samples brighten slightly. XPS analysis shows that irradiation of Fo90 produces metallic Fe. Analytical SEM and TEM measurements confirm that reddening in the Fo90 olivine samples correlates with the production of "nanophase" metallic Fe (npFe0) grains, 20-50 nm in size. The reddening observed in the SiO2 sample is consistent with the formation of SiO or other SiOx species that absorb in the visible. The weak spectral brightening induced by laser irradiation of SiO2 is consistent with a change in surface topography of the sample. The darkening observed in the olivine samples is likely caused by the formation of larger npFe0 particles, such as the 100-400 nm diameter npFe0 identified during our TEM analysis of Fo90 samples. The Fo90 reflectance spectra are qualitatively similar to those in previous experiments suggesting that in all cases formation of npFe0 is causing the spectral alteration. Finally, we find that the accumulation of successive laser pulses cause continued sample darkening in the Vis-NIR, which suggests that repeated surface impacts are an efficient way to darken airless body surfaces.

Airless planetary bodies↗