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At least 37 records · Page 2

Flight test investigation of the vortex wake characteristics behind a Boeing 727 during two-segment and normal ILS approaches (A joint NASA/FAA report)

Flight tests were performed to evaluate the vortex wake characteristics of a Boeing 727 aircraft during conventional and two-segment instrument landing approaches. Smoke generators were used for vortex marking. The vortex was intentionally intercepted by a Lear Jet and a Piper Comanche aircraft. The vortex location during landing approach was measured using a system of phototheodolites. The tests showed that at a given separation distance there are no readily apparent differences in the upsets resulting from deliberate vortex encounters during the two types of approaches. The effect of the aircraft configuration on the extent and severity of the vortices is discussed.

Barber, M. R.↗

Baseline measurement of the noise generated by a short-to-medium range jet transport flying standard ILS approaches and level flyovers

The results of baseline noise flight tests are presented. Data are given for a point 1.85 kilometers (1.0 nautical mile) from the runway threshold, and experimental results of level flyover noise at altitudes of 122 meters (400 feet) and 610 meters (2,000 feet) are also shown for several different power levels. The experimental data are compared with data from other sources and reasonable agreement is noted. A description of the test technique, instrumentation, and data analysis methods is included.

Hastings, E. C., Jr.↗

Airline pilot scan patterns during simulated ILS approaches

A series of instrument landing system approaches were conducted using seven airline-rated Boeing 737 pilots in a Federal Aviation Administration qualified simulator. The test matrix included both manual and coupled approaches with and without atmospheric turbulence in Category II visibility conditions. A nonintrusive oculometer system was used to track the pilot eye-point-of-regard throughout the approach. The results indicate that, in general, the pilots use different scan techniques for the manual and coupled conditions and that the introduction of atmospheric turbulence does not greatly affect the scan behavior in either case. The pilots consistently ranked the instruments in terms of most used to least used. The ranking obtained from the oculometer data agrees with the pilot ranking for the flight director and airspeed, the most important instruments. However, the pilots apparently ranked the other instruments in terms of their concern for information rather than according to their actual scanning behavior.

Spady, A. A., Jr.↗

Advances in cryogenic engineering. Volume 33 - Proceedings of the Cryogenic Engineering Conference, Saint Charles, IL, June 14-18, 1987

Papers are presented on superconductivity applications including magnets, electronics, rectifiers, magnet stability, coil protection, and cryogenic techniques. Also considered are insulation, heat transfer to liquid helium and nitrogen, heat and mass transfer in He II, superfluid pumps, and refrigeration for superconducting systems. Other topics include cold compressors, refrigeration and liquefaction, magnetic refrigeration, and refrigeration for space applications. Papers are also presented on cryogenic applications, commercial cryogenic plants, the properties of cryogenic fluids, and cryogenic instrumentation and data acquisition.

Fast, R. W.↗

Heat transfer in gas turbine engines and three-dimensional flows; Proceedings of the Symposium, ASME Winter Annual Meeting, Chicago, IL, Nov. 27-Dec. 2, 1988

The present conference on heat transfer characteristics of gas turbines and three-dimensional flows discusses velocity-temperature fluctuation correlations at the flow stagnation flow of a circular cylinder in turbulent flow, heat transfer across turbulent boundary layers with pressure gradients, the effect of jet grid turbulence on boundary layer heat transfer, and heat transfer characteristics predictions for discrete-hole film cooling. Also discussed are local heat transfer in internally cooled turbine airfoil leading edges, secondary flows in vane cascades and curved ducts, three-dimensional numerical modeling in gas turbine coal combustor design, numerical and experimental results for tube-fin heat exchanger airflow and heating characteristics, and the computation of external hypersonic three-dimensional flow field and heat transfer characteristics.

Elovic, E.↗

Recent advances in control of nonlinear and distributed parameter systems, robust control, and aerospace control applications; Proceedings of the Symposium, ASME Winter Annual Meeting, Chicago, IL, Nov. 27-Dec. 2, 1988

Papers are presented on the control of distributed parameter systems using the coprime factorization approach, a hierarchy of geometrically exact models of rotating beams, and the temperature stability of a flux modulated radiant slab. Also considered are a network representation for robots, recovery from controller bounding, and the design of controllers for robust tracking and model following. Other topics include an active control law synthesis for flexible aircraft, the low-order control of large aerospsace structures using residual model filters, and a comparison of component cost methods for component model reduction.

Bentsman, Joseph↗

SIAM Conference on Parallel Processing for Scientific Computing, 4th, Chicago, IL, Dec. 11-13, 1989, Proceedings

Attention is given to such topics as an evaluation of block algorithm variants in LAPACK and presents a large-grain parallel sparse system solver, a multiprocessor method for the solution of the generalized Eigenvalue problem on an interval, and a parallel QR algorithm for iterative subspace methods on the CM2. A discussion of numerical methods includes the topics of asynchronous numerical solutions of PDEs on parallel computers, parallel homotopy curve tracking on a hypercube, and solving Navier-Stokes equations on the Cedar Multi-Cluster system. A section on differential equations includes a discussion of a six-color procedure for the parallel solution of elliptic systems using the finite quadtree structure, data parallel algorithms for the finite element method, and domain decomposition methods in aerodynamics. Topics dealing with massively parallel computing include hypercube vs. 2-dimensional meshes and massively parallel computation of conservation laws. Performance and tools are also discussed.

Dongarra, Jack↗

A Chandra X-Ray Study of NGC 1068 IL the Luminous X-Ray Source Population

We present an analysis of the compact X-ray source population in the Seyfert 2 galaxy NGC 1068, imaged with a approx. 50 ks Chandra observation. We find a total of 84 compact sources on the S3 chip, of which 66 are located within the 25.0 B-mag/arcsec isophote of the galactic disk of NGC 1068. Spectra have been obtained for the 21 sources with at least 50 counts and modeled with both multicolor disk blackbody and power-law models. The power-law model provides the better description of the spectrum for 18 of these sources. For fainter sources, the spectral index has been estimated from the hardness ratio. Five sources have 0.4 - 8 keV intrinsic luminosities greater than 10(exp 39)ergs/ s, assuming that their emission is isotropic and that they are associated with NGC 1068. We refer to these sources as intermediate-luminosity X-ray objects (ISOs). If these five sources are X-ray binaries accreting with luminosities that are both sub-Eddington and isotropic, then the implied source masses are approx greater than 7 solar mass, and so they are inferred to be black holes. Most of the spectrally modeled sources have spectral shapes similar to Galactic black hole candidates. However, the brightest compact source in NGC 1068 has a spectrum that is much harder than that found in Galactic black hole candidates and other ISOs. The brightest source also shows large amplitude variability on both short-term and long-term timescales, with the count rate possibly decreasing by a factor of 2 in approx. 2 ks during our Chundra observation, and the source flux decreasing by a factor of 5 between our observation and the grating observations taken just over 9 months later. The ratio of the number of sources with luminosities greater than 2.1 x 10(exp 38) ergs/s in the 0.4 - 8 keV band to the rate of massive (greater than 5 solar mass) star formation is the same, to within a factor of 2, for NGC 1068, the Antennae, NGC 5194 (the main galaxy in M51), and the Circinus galaxy. This suggests that the rate of production of X-ray binaries per massive star is approximately the same for galaxies with currently active star formation, including "starbursts."

Smith, David A.↗

Visions for Space Exploration: ILS Issues and Approaches

This viewgraph presentation reviews some of the logistic issues that the Vision for Space Exploration will entail. There is a review of the vision and the timeline for the return to the moon that will lead to the first human exploration of Mars. The lessons learned from the International Space Station (ISS) and other such missions are also reviewed.

Watson, Kevin↗

Dual Anion-Cation Crosslinked Poly(ionic liquid) Composite Membranes for Enhanced CO2 Separation

Herein, we propose a strategy of developing novel dual anionic-cationic crosslinked poly(IL)-IL composite membranes via a photopolymerization method for enhanced CO2 separations. These are the first examples of dually photopolymerized anionic-cationic poly(IL)-IL composite systems, in which the backbones of poly(IL)s feature both pendant anions and cations without any mobile counterions unlike poly(IL) reported so far in the literature. A new type of dual photopolymerizable anionic-cationic IL (DIL) monomer having methacrylate functional group tethered with highly delocalized sulfonimide anion (–SO2–N(-)–SO2–C7H7) and a vinylimidazolium counterion ([C4(vim)](+)) was successfully synthesized and photopolymerized with four distinct amounts of free IL with a structurally analogous cation ([C4(mim)][Tf2N]) and 20 wt% PEGDA crosslinker, offering novel composite matrices. Further, the structure-property relationships as well as gas separation behaviors of the four newly developed dual anioniccationic poly(IL)-IL composite membranes were extensively characterized by FT-IR, DSC, and XRD. All of the newly developed dual anionic-cationic poly(IL)-IL composite membranes displayed outstanding permselectivites for CO2/CH4, CO2/N2, and CO2/H2 gas pairs together with reasonable CO2 permeabilities. As a result, all the dual anionic-cationic poly(IL)-IL composite membranes outperformed the common poly(IL)-IL systems in the upper bound limit plots with best CO2 permeability of 40 barrer and CO2/CH4 permselectivity of 85. This study may pave a new platform to explore countless potential poly(IL)-IL composites for selective separation of CO2 from flue gas, natural gas, and syngas streams.

Irshad Kammakakam↗

Whole Blood Activation Results in Enhanced Detection of T Cell and Monocyte Cytokine Production by Flow Cytometry

An excellent monitor of the immune balance of peripheral circulating cells is to determine their cytokine production patterns in response to stimuli. Using flow cytometry a positive identification of cytokine producing cells in a mixed culture may be achieved. Recently, the ability to assess cytokine production following a wholeblood activation culture has been described. We compared whole blood culture to standard PBMC culture and determined the individual cytokine secretion patterns for both T cells and monocytes via flow cytometry. For T cells cytokine assessment following PMA +ionomycin activation: (1) a significantly greater percentages of T cells producing IFNgamma and IL-2 were observed following whole-blood culture; (2) altered T cell cytokine production kinetics were observed by varying whole blood culture times. In addition, a four-color cytometric analysis was used to allow accurate phenotyping and quantitation of cytokine producing lymphocyte populations. Using this technique we found IFNgamma production to be significantly elevated in the CD3+/CD8+ T cell population as compared to the CD3+/CD8- population following five hours of whole blood activation. Conversely, IL-2 and IL-10 production were significantly elevated in the CD3+/CD8- T cell population as compared to the CD3+/CD8+ population. Monocyte cytokine production was assessed in both culture systems following LPS activation for 24 hours. A three-color flow cytometric was used to assess two cytokines in conjunction with CD 14. The cytokine pairs used for analysis were IL-1a/IL-12, and IL-10ITNFa. Nearly all monocytes were stimulated to produce IL-1a, IL-12 and TNFalpha equally well in both culture systems. Monocyte production of IL-10 was significantly elevated following whole blood culture as compared to PBMC culture. IL-12 producing monocytes appeared to be a distinct subpopulation of the IL-1a producing set, whereas IL-10 and TNFa producing monocytes were largely mutually exclusive. IL-10 and TNFa producing monocytes may represent functionally different monocyte subsets with distinct functions. Whole blood culture eliminates the need to purify cell populations prior to culture and may have significant utility for the routine monitoring of the cytokine balances of the peripheral blood T cell and monocyte populations. In addition, there are distinct advantages to performing whole-blood (WB) activation as compared to PBMC activation. These advantages would include retaining all various cell-cell interactions as well as any soluble factors present in serum that influence cell activation. It is likely that the altered cytokine production observed following whole blood culture more accurately represents the in-vivo immune balance.

Sams, Clarence F.↗

Whole Blood Activation Results in Altered T Cell and Monocyte Cytokine Production Profiles by Flow Cytometry

An excellent monitor of the immune balance of peripheral circulating cells is to determine their cytokine production patterns in response to stimuli. Using flow cytometry, a positive identification of cytokine producing cells in a mixed culture may be achieved. Recently, the ability to assess cytokine production following a whole-blood activation culture has been described. In this study, whole blood activation was compared to traditional PBMC activation and the individual cytokine secretion patterns for both T cells, T cell subsets and monocytes was determined by flow cytometry. RESULTS: For T cell cytokine assessment (IFNg/IL-10 and IL-21/L-4) following PMA +ionomycin activation: (1) a Significantly greater percentages of T cells producing IFNgamma and IL-2 were observed following whole-blood culture and (2) altered T cell cytokine production kinetics were observed by varying whole blood culture times. Four-color analysiS was used to allow assessment of cytokine production by specific T cell subsets. It was found that IFNgamma production was significantly elevated in the CD3+/CD8+ T cell population as compared to the CD3+/CD8- population following five hours of whole blood activation. Conversely, IL-2 and IL-10 production were Significantly elevated in the CD3+/CD8- T cell population as compared to the CD3+/CD8+ population. Monocyte cytokine production was assessed in both culture systems following LPS activation for 24 hours. A three-color flow cytometric was used to assess two cytokines (IL-1a/IL-12 and TNFa/IL-10) in conjunction with CD14. Nearly all monocytes were stimulated to produce IL-1a, IL-12 and TNFa. equally well in both culture systems, however monocyte production of IL-10 was significantly elevated in whole blood culture as compared to PBMC culture. IL-12 producing monocytes appeared to be a distinct subpopulation of the IL-1a producing set, whereas IL-10 and TNFa producing monocytes were largely mutually exclusive. IL-10 and TNFa producing monocytes may represent distinct monocyte subsets with unique functions. CONCLUSIONS: Whole blood culture eliminates the need to purify cell populations prior to culture and may have Significant utility for the routine monitoring of the cytokine balances of the peripheral blood T cell and monocyte populations. In addition, there are distinct advantages to performing whole-blood (WB) activation as compared to PBMC activation. These advantages would include retaining all various cell-cell interactions as well as any soluble factors present in serum that influence cell activation. In this study, alterations in cytokine production are demonstrated between whole blood and PBMC activation. It is likely that whole blood activation more accurately represents the in-vivo immune balance than PBMC activation.

Crucian, Brian E.↗

Protein Crystallization Using Room Temperature Ionic Fluids

The ionic liquids (ILs) 1-butyl-3-methylimidizolium chloride (C4mim-C1), 1-butyl-3- methylimidizolium diethyleneglycol monomethylethersulfate ([C4mim]DEMGS), and 1-butyl-1 -methylpyrollidinium dihydrogenphosphate ([p1,4]dhp) were tested for their effects on the crystallization of the proteins canavalin, beta-lactoglobulin B, xylanase, and glucose isomerase, using a standard high throughput screen. The crystallization experiments were set up with the ILs added to the protein solutions at 0.2 and 0.4 M final concentrations. Crystallization droplets were set up at three proteixprecipitant ratios (1:1, 2:1, and 4:l), which served to progressively dilute the effects of the screen components while increasing the equilibrium protein and IL concentrations. Crystals were obtained for all four proteins at a number of conditions where they were not obtained from the IL-free control experiment. Over half of the protein-IL combinations tested had more successful outcomes than negative, where the IL-free crystallization was better than the corresponding IL-containing outcome, relative to the control. One of the most common causes of a negative outcome was solubilization of the protein by the IL, resulting in a clear drop. In one instance, we were able to use the IL-induced solubilizing to obtain beta-lactoglobulin B crystals from conditions that gave precipitated protein in the absence of IL. The results suggest that it may be feasible to develop ILs specifically for the task of macromolecule crystallization.

Pusey, Marc L.↗

Novel Aprotic Heterocyclic Anion-based Ionic Liquids for Carbon Dioxide Capture

Ionic liquids (ILs), or organic salts with melting points at or below room temperature are an incredibly versatile class of materials with many possible applications for NASA’s exploration needs. In particular, appropriately designed ILs can have a high affinity for absorbing carbon dioxide, making them excellent candidates for use as liquid sorbents in cabin air revitalization systems. Unfortunately, many of these ILs have high viscosity, which is only exacerbated by carbon dioxide uptake. High viscosity limits the rate of carbon dioxide uptake. A new class of IL sorbent containing aprotic heterocyclic anions (AHA) has been shown to offer excellent carbon dioxide uptake without a corresponding increase in viscosity. However, there is a paucity of sorption data for these AHA ILs at low partial pressures of carbon dioxide. This paper reports on the characterization of sorption behavior for AHA ILs previously reported in the literature at low concentrations of carbon dioxide and the synthesis and characterization of new, water miscible AHA ILs. Characterization of the new ILs includes measurement of density, viscosity, thermal stability, carbon dioxide capacity, carbon dioxide absorption kinetics, and water-IL vapor liquid equilibrium. Overall, these news ILs are promising alternatives to many of the commonly used IL sorbents.

Eric Townsend Fox↗

Influence of spaceflight on the production of interleukin-3 and interleukin-6 by rat spleen and thymus cells

Six adult male Sprague-Dawley rats were flown on the 7-day US space shuttle mission STS-54. After flight, the spleen and thymus from each animal were assayed for the capacity to secrete the cytokines interleukin-3 (IL-3) and IL-6. Spleen and thymus cells were incubated for 48 h in the presence of 5 microgram/ml of concanavalin A or 2 microgram/ml of bacterial lipopolysaccharide to stimulate the production of IL-3 and IL-6. IL-3 activity was measured using the IL-3/colony-stimulating-factor-dependent cell line 32D. IL-6 activity was measured using the IL-6-dependent cell line 7TD1. Spleen and thymus cells harvested from flown rats secreted significantly higher titers of biologically active IL-3 compared with ground control rats. Spaceflight significantly enhanced IL-6 production by thymus, but not spleen, cells. The results of this study demonstrate that spaceflight can enhance the production of certain cytokines by cells of the immune system.

Miller, Edwin S.↗

Mitogenic signal transduction in T lymphocytes in microgravity

The activation by concanavalin A Con A of human peripheral blood lymphocytes (PBLs) in the presence of monocytes as accessory cells was investigated in cultures exposed to microgravity conditions in Spacelab. Activation of T cells was measured as incorporation of [3H]thymidine into DNA, secretion of interleukin-2 (IL-2), and interferon-gamma, and expression of IL-2 receptors. Whereas, as discovered in earlier experiments, the activation of resuspended T cells is strongly inhibited, activation of cells attached to microcarrier beads is more than doubled in microgravity. The results suggest that the depression of the activation in resuspended cells may be attributed to a malfunction of monocytes acting as accessory cells. In fact, although the ultrastructure of resuspended monocytes is not altered in microgravity, the secretion of IL-1 is strongly inhibited. Our data suggest that (1) IL-2 is produced independently of IL-1, (2) IL-1 production is triggered only when monocytes (and lymphocytes?) adhere to microcarriers, (3) the expression of IL-2 receptors depends on IL-1, and (4) provided sufficient IL-1 is available, activation is enhanced in microgravity. Finally, cultures of resuspended PBLs and monocytes in microgravity constitute a complete and natural system in which monocytes are not operational. This may be useful for studies of the role of accessory cells and cell-cell interactions in T lymphocyte activation.

STS-40 Shuttle Project↗