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23 records · Page 2

Two Alternative Splicing Variants of AtERF73/HRE1, HRE1α and HRE1β, Have Differential Transactivation Activities in Arabidopsis

AtERF73/HRE1 is an AP2/ERF transcription factor in Arabidopsis and has two distinct alternative splicing variants, HRE1α and HRE1β. In this study, we examined the differences between the molecular functions of HRE1α and HRE1β. We found that HRE1α and HRE1β are both involved in hypoxia response and root development and have transactivation activity. Two conserved motifs in the C-terminal region of HRE1α and HRE1β, EELL and LWSY-like, contributed to their transactivation activity, specifically the four E residues in the EELL motif and the MGLWS amino acid sequence at the end of the LWSY-like motif. The N-terminal region of HRE1β also showed transactivation activity, mediated by the VDDG motif, whereas that of HRE1α did not. The transactivation activity of HRE1β was stronger than that of HRE1α in Arabidopsis protoplasts. Both transcription factors transactivated downstream genes via the GCC box. RNA-sequencing analysis further supported that both HRE1α and HRE1β might regulate gene expression associated with the hypoxia stress response, although they may transactivate different subsets of genes in downstream pathways. Our results, together with previous studies, suggested that HRE1α and HRE1β differentially transactivate downstream genes in hypoxia response and root development in Arabidopsis.

36 MATERIALS SCIENCE↗

Vectorization of CMSSW offline software

The CMS experiment has been utilizing vectorization, or SIMD, in parts of its data processing applications for over a decade. On x86 platforms the vectorization level is still SSE3. In the past attempts to use wider vector instruction sets such as AVX or AVX-512 have, in practice, not resulted in improvements in the overall event processing throughput, because the CPUs scale down their frequency when processing AVX instructions. In addition, a notable part of the global pool of CMS’ resources has been old systems either not supporting AVX, or where the CPU frequency downscaling impacts all cores of the CPU. CMS has nevertheless continued to vectorize more of its application code, and in this work we review profiling methods we have found effective to find out pieces of code that would benefit from vectorization, and techniques to transform those codes such that the GCC compiler is able to auto-vectorize those codes. The build system used for CMSSW, Scram, has also been enha nced to be able to build code for multiple CPU microarchitectures such that the shared libraries of desired microarchitecture level can be loaded based on the CPU of the system. This multi-microarchitecture setup is invisible to the workflow management system, which makes its deployment straightforward. We describe in detail how this multi-microarchitecture build is set up, and measure the impact of using wider vector units than SSE3 on the event processing throughput of CMS applications such as simulation and reconstruction on recent x86 CPUs.

Gartung, Patrick↗

Generalizing mkFit and its Application to HL-LHC

mkFit is an implementation of the Kalman filter-based track reconstruction algorithm that exploits both thread- and data-level parallelism. In the past few years the project transitioned from the R&D phase to deployment in the Run-3 offline workflow of the CMS experiment. The CMS tracking performs a series of iterations, targeting reconstruction of tracks of increasing difficulty after removing hits associated to tracks found in previous iterations. mkFit has been adopted for several of the tracking iterations, which contribute to the majority of reconstructed tracks. When tested in the standard conditions for production jobs, speedups in track pattern recognition are on average of the order of 3.5x for the iterations where it is used (3-7x depending on the iteration). Multiple factors contribute to the observed speedups, including vectorization and a lightweight geometry description, as well as improved memory management and single precision. Efficient vectorization is achieved with both the icc and the gcc (default in CMSSW) compilers and relies on a dedicated library for small matrix operations, Matriplex, which has recently been released in a public repository. While the mkFit geometry description already featured levels of abstraction from the actual Phase-1 CMS tracker, several components of the implementations were still tied to that specific geometry. We have further generalized the geometry description and the configuration of the run-time parameters, in order to enable support for the Phase-2 upgraded tracker geometry for the HL-LHC and potentially other detector configurations. The implementation strategy and high-level code changes required for the HL-LHC geometry are presented. Speedups in track building from mkFit imply that track fitting becomes a comparably time consuming step of the tracking chain.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Using phenology to unravel differential soil water use and productivity in a semiarid savanna

Savannas are water-limited ecosystems characterized by two dominant plant types: trees and an understory primarily made up grass. Different phenology and root structures of these plant types complicate how savanna primary productivity responds to changes in water availability. We tested the hypothesis that productivity in savannas is controlled by the temporal and vertical distribution of soil water content (SWC) and differences in growing season length of understory and tree plant functional types. To quantify the relationship between tree, understory, and savanna-wide phenology and productivity, we used PhenoCam and satellite observations surrounding an eddy covariance tower at a semiarid savanna site in Arizona, USA. We distinguished between SWC across two different depth intervals (shallow, <0–30 cm and deep, >30–100 cm). We found that tree greenness increased with SWC at both depths, while understory greenness was only sensitive to the shallower SWC measurements. Onset of ecosystem dormancy, estimated from satellite observations close to the eddy covariance tower, explained more variability in annual gross primary productivity (GPP) than in other phenometrics. Higher SWC led to an extended growing season, caused by delayed dormancy in trees, but the understory showed no evidence of delayed dormancy in wetter periods. We infer that the timing of ecosystem scale dormancy, driven by trees, is important in understanding changes in a savanna's GPP. These findings highlight the important effects of rainfall during the winter. These findings suggest that savanna GPP is conditional on different responses to moisture availability in each of the dominant vegetation components.

54 ENVIRONMENTAL SCIENCES↗

Leaf phenology data at The Morton Arboretum Forestry Plots 2019-2023

We are collecting long-term leaf phenology data at The Morton Arboretum to determine seasonal patterns of leaf production in trees. This data on leaf phenology will be integrated with other ongoing data streams to create a connection between above- and below-ground tree processes. This data package contains raw and smooth outputs from phenology data, as well as extracted phenophase dates (i.e., start, peak, and end of season): the raw and smooth outputs from the PhenoCam GUI can be found in the "leafRaw.csv" and "leafSmooth.csv" files, respectively, and the extracted phenophase dates can be found in the "leafPhenophaseDates2019-2023.csv" file. Extracted phenophase dates for evergreen species in 2023 are currently unavailable, and the files will be updated once they are extracted. Additional information on units and other file-level metadata can be found within each data file's respective data dictionary, and metadata for each of the 23 surveyed plots can be found within the "Location_metadata.csv" file. While the "leafRaw.csv" and the "leafSmooth.csv" files contain all data for all species, the "leafPhenophaseDates2019-2023.csv" file currently excludes the dates for evergreen species in 2023. Another version of the file will be added as those dates are extracted.

54 ENVIRONMENTAL SCIENCES↗