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26 records · Page 2
All sourcesNASA NTRSDOE OSTI
DOE OSTI2024

Comparison of Intact Fish Skin Graft and Allograft as Temporary Coverage for Full-Thickness Burns: A Non-Inferiority Study

The extent and depth of burn injury may mandate temporary use of cadaver skin (allograft) to protect the wound and allow the formation of granulation tissue while split-thickness skin grafts (STSGs) are serially harvested from the same donor areas. However, allografts are not always available and have a high cost, hence the interest in identifying more economical, readily available products that serve the same function. This study evaluated intact fish skin graft (IFSG) as a temporary cover to prepare the wound bed for STSG application. Thirty-six full-thickness (FT) 5 × 5 cm burn wounds were created on the dorsum of six anesthetized Yorkshire pigs on day -1. To mimic the two-stage clinical situation, on day 0, wounds were excised down to a bleeding wound bed and a temporary cover (either IFSG or cadaver porcine skin) was applied; then, on day 7, wounds were debrided to a viable wound bed prior to the application of autologous 1.5:1 meshed STSG (mSTSG). Rechecks were performed on days 14, 21, 28, 45, and 60 with digital images, non-invasive measurements, and punch biopsies. The IFSG created a granulated wound bed receptive to the application of an mSTSG. FT burn wounds treated with an IFSG had similar outcome measures, including contraction rates, trans-epidermal water loss (TEWL) measurements, hydration, and blood perfusion levels, compared to cadaver skin-treated burn wounds. Pathology scoring indicated significant differences between the allograft- and IFSG-treated wounds on day 7, with the IFSG having increased angiogenesis, granulation tissue formation, and immune cells. Pathology scoring indicated no significant differences once mSTSGs were applied to wounds. The IFSG performed as well as cadaver skin as a temporary cover and was not inferior to the standard of care, suggesting the potential to transition IFSGs into clinical use for burns.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2021

Deep Learning for Fish Identification from Sonar Data: CRADA 481 [Abstract only]

To help solve the challenges of hydropower energy production related to the potential for eel injury and mortality from passage through hydropower turbines, we will develop a deep learning method for identifying migrating eels from imaging sonar. This project continues with a prior project conducted by the Pacific Northwest National Laboratory (PNNL) and the Electric Power Research Institute (EPRI) in FY2018-2019. The proposed method employs Convolution Neural Network (CNN), a powerful deep learning method for image classification, to distinguish between images of eels and non-eel moving objects. We propose to collect more laboratory data and add more existing field data to train a powerful deep learning model. In addition to eels and sticks as classified in previous studies, we will add images containing several non-eel fish species and macrophyte mats to the training data. A multi-class classification model will be developed to distinguish these objects. Object detection algorithm will be explored and developed to locate and identify multiple objects in each sonar frame. Motion analysis will be performed to track the movement of objects in sonar video clips. We will also improve the data conversion algorithm so that it can read in both DIDSON and ARIS (both are imaging sonars developed by Sound Metrics Corp) data files and convert them to images with comparably high resolution, regardless of the varying detection ranges in different environments. The developed algorithms will be packaged as a software with a graphic user interface. The software will be evaluated by external collaborators in the field. The developed framework can be generalized for automatic monitoring of fish passage and migration using other imaging sonars like ARIS and will benefit the design and operation of ecologically friendly hydroelectric projects. The developed wavelet and CNN model configuration parameters can potentially be transferred to lamprey detection in similar riverine environments.

13 HYDRO ENERGY↗
DOE OSTI2022

PNNL CRADA No. 488, Amendment 2 with Natel Energy, Inc.: The Center Sender

Under the Cooperative Research and Development Agreement (CRADA) No. 488, awarded to Natel Energy, Inc. (Natel) for third place in the Fish Protection Prize 2020 initiative, research was conducted on the Center Sender concept, a simple physical or combined physical and electrical device intended to guide fish to the safest path through a hydropower intake or water diversion. Applying this to a hydropower turbine, the Center Sender can be designed to divert fish to the center of the turbine, where blade velocities are slower and the risk of severe injuries due blade strike, pinching, and grinding are reduced.

13 HYDRO ENERGY↗
DOE OSTI2022

Survival estimates across five life stages of redfin ( Perca fluviatilis ) exposed to simulated pumped-storage hydropower stressors

The global prevalence of pumped-storage hydropower (PSH) is expected to grow exponentially as countries transition to renewable energy sources. Compared to conventional hydropower, little is currently known regarding PSH impacts on aquatic biota. This study estimated the survival of five life stages (egg, two larval stages, juvenile and adult) of redfin (European) perch (Perca fluviatilis) following passage through a PSH facility during the pumping phase. This was achieved by simulating the individual stressors expected to occur during passage through a 2000-MW PSH facility using laboratory-simulated (shear strain and extreme compression) and modelling (blade strike, BS) approaches. Our results indicate that redfin could survive the shear, pressure and BS stressors expected within the PSH facility, but impacts varied among life stages. Juvenile survival was >70% across all shear strain rates, while the survival of eggs and larvae declined markedly as strain rate increased. All life stages had high survival when exposed to rapid compression and BS. The high survival of redfin to the stressors tested suggests the PSH facility could facilitate the passage of redfin during the pumping phase from the lower to the higher elevation reservoir. This outcome would be welcomed in situations where the species is native, but could have adverse implications for the conservation of native biota where the species is considered a pest.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2022

Safe passage of American Eels through a novel hydropower turbine

Abstract Objective Study the effects of downstream passage through a novel turbine designed for fish safety, the Restoration Hydro Turbine (RHT), on American Eels Anguilla rostrata in a recirculating turbine test facility. Methods A 55‐cm‐diameter RHT was operated under 10 m of hydraulic head and 667 revolutions/min. In total, 131 eels were passed through the turbine and 43 eels were used as experimental controls (length = 33.9–65.5 cm). High‐speed video of passage through the runner region was captured for 89% of turbine‐passed eels, and injury and behavioral effects were recorded immediately before and after passage, as well as after a 48‐h holding period. A subset of 37 eels was additionally examined with X‐ray imaging for internal injuries. Result The 48‐h survival rate for both treatment and control groups was 100%, with no major internal or external injuries present after the holding period. Conclusion This is a substantial improvement over eel survival rates through conventional Kaplan and Francis turbines, which may range from 40% to 95%, and suggests that hydropower turbines designed for safe downstream fish passage could be implemented without major impacts to eels.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2025

Feed status and skin injury modulate immunopathology, global gene expression, and survival in channel catfish during virulent Aeromonas hydrophila infection

Introduction VirulentAeromonas hydrophilais a major pathogen in channel catfish (Ictalurus punctatus), that causes motileAeromonassepticemia and significant economic losses. We investigated the effect of feeding status and skin integrity on the host immune response, disease survival, and gastrointestinal pathology following a vAh challenge. Methods Using a bath immersion model, channel catfish were divided into four treatment groups: fin clipped and fed (FCF), fin clipped but not fed (FCN), not fin clipped but fed (NCF), and not fin clipped nor fed (NCN) alongside non-challenged control groups The FCF and NCF groups were fed 2 h prior to the challenge, but the FCN and NCN groups were not. Survival analysis, histopathological assessment, and RNA sequencing were conducted across groups at different time intervals throughout the vAh challenge. Results Survival rates were lowest in the FCF and FCN groups (30% and 23% survival, respectively), suggesting that both feeding and skin damage contributed to disease severity. Histopathological analyses revealed more severe intestinal and gastric lesions in fed groups, characterized by epithelial necrosis, hemorrhage, and edema. Transcriptomic analysis among the groups identified significant differentially expressed genes associated with inflammation, apoptosis, and metabolic stress, with notable upregulation of interleukin 1-beta (il-1β), and complement C3 (c3). Gene ontology enrichment highlighted distinct immune activation patterns between fed and unfed groups, with enhanced pathogen recognition and pro-inflammatory responses in unfed fish. Discussion These findings suggest feeding prior to infection may exacerbate disease pathology, potentially by creating a physiological state conducive to facilitate pathogen proliferation and dampened early immune responses, whereas short-term fasting appears to promote early immune activation. This study provides novel insights into the complex interplay between feed status, physical injury, and immune response to vAh infection.

Immunology↗
NASA NTRS1996

Rapid Bacterial Testing for Spacecraft Water

Evaluations of the fluorogenic stains and probes will continue. E. coli 0157:H7 will be used as the reference strain for optimizing protocols. We anticipate the continued use of the fluorescent antibodies (TRITC and FITC labeled) in conjunction with CTC, Rhl23, DiBAC4(3), DAPI and acridine orange. Chemunex, the manufacturer of the ChemScan analyzer system, also makes a fluorogenic probe, Chemchrome B, which will be incorporated into the suite of probes to evaluate once their system is on site. Regardless of the combination of stains and probes all will be evaluated on membrane filters. Development of a FISH protocol that will be applicable to our conditions will be continued. Complimentary 16s rRNA probes to Ps. aeruginosa and currently in our laboratory will be evaluated first. Once this protocol has been adequately optimized other probes will be ordered for u a select number of other species. Currently, protocols to evaluate the effects of disinfection and the resulting lethality, injury on stain and/or probe specificity and reliability are being developed. E. coli 0157:H7 is the reference strain and chlorine the disinfectant the reference protocol is being developed around. Upon completion of this work, the resulting protocol will be extended to other species and disinfectants (e.g., iodine). Similar disinfectant experiments will then be conducted on the same species after starvation to evaluate the effects of starvation on disinfection resistance and the applicability of the stains and probes. Development of the immunomagnetic separation system will continue. Combined with the rapid methods described above, with enumeration by the ChemScan, we anticipate that this will provide a highly sensitive technique for the detection of specific, active bacteria.

Lisle, John T.↗
DOE OSTI2020

Gambusia holbrooki Survive Shear Stress, Pressurization and Avoid Blade Strike in a Simulated Pumped Hydroelectric Scheme

Pumped hydroelectric energy storage (PHES) projects are being considered worldwide as a means of achieving political renewable energy targets in a way that stabilises baseload energy supply from often intermittent renewable energy sources. Unlike a conventional hydroelectric system that only pass water in a downstream direction, a feature of PHES is that it relies on the bi-directional flow of water. In some cases this flow can be across different waterbodies or catchments, posing a risk of inadvertently expanding the range of aquatic animals like fish. The risk of this happening depends on the likelihood of survival of individuals, which remains poorly understood for turbines that are pumping rather than generating. This study quantified the survival of a globally widespread and invasive poeciliid fish, Eastern gambusia (Gambusia holbrooki) when exposed hydraulic stresses characteristic of what would be experienced through a PHES during the pumping phase. A shear flume and hyperbaric chamber were used to expose fish to different strain rates and rapid and sustained pressurisation. A blade strike model was also used to predict survival of fish passing through a Francis dual turbine / pump. The ranges simulated were based on design and operational conditions provided for a PHES scheme being proposed in south-eastern Australia. All gambusia tested survived extremely high (up to 7600 kPa gauge pressure) pressurisation, high levels of shear stress (up to 1853 s -1 ), and the majority (> 93 %) were unlikely to be struck by a turbine blade. Given their tolerance to these extreme simulated stresses, we conclude that gambusia will likely survive passage through a PHES scheme. Therefore, where a new PHES poses the risk of inadvertently expanding the range of gambusia or similar poeciliid species, measures to minimise their spread or mitigate their ecosystem impacts should be considered.

25 ENERGY STORAGE↗