Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “Evidence Lower Bound”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

36 records · Page 2
All sourcesNASA NTRSDOE OSTI
NASA NTRS2014

Extreme Thunderstorms as Seen by Satellite

Extreme events by their nature fall outside the bounds of routine experience. With imperfect or ambiguous measuring systems, it is appropriate to question whether an unusual measurement represents an extreme event or is the result of instrument errors or other sources of noise. About three weeks after the Tropical Rainfall Measuring Mission (TRMM) satellite began collecting data in Dec 1997, a thunderstorm was observed over northern Argentina with 85 GHz brightness temperatures below 50 K and 37 GHz brightness temperatures below 70 K (Zipser et al. 2006). These values are well below what had previously been observed from satellite sensors with lower resolution. The 37 GHz brightness temperatures are also well below those measured by TRMM for any other storm in the subsequent 16 years. Without corroborating evidence, it would be natural to suspect a problem with the instrument, or perhaps an irregularity with the platform during the first weeks of the satellite mission. But the TRMM satellite also carries a radar and a lightning sensor, both confirming the presence of an intense thunderstorm. The radar recorded 40+ dBZ (decibels relative to Z) reflectivity up to about 19 km altitude. More than 200 lightning flashes per minute were recorded. That same storm's 19 GHz brightness temperatures below 150 K would normally be interpreted as the result of a low-emissivity water surface (e.g., a lake, or flood waters) if not for the simultaneous measurements of such intense convection. This paper will examine records from TRMM and related satellite sensors including SSMI and AMSR-E to find the strongest signatures resulting from thunderstorms, and distinguishing those from sources of noise. The lowest brightness temperatures resulting from thunderstorms as seen by TRMM have been in Argentina in November and December. For SSMI sensors carried on five DMSP satellites examined so far, the lowest thunderstorm-related brightness temperatures have been from Argentina in November - December and from Minnesota in June-July. The Minnesota cases were associated with spotter reports of large hail, significant severe wind, and tornadoes. Those locations have the record holders for each satellite. This paper will show examples of cases with the lowest brightness temperatures, and map the locations of these and other storms with brightness temperatures nearly as low. Higher resolution data from the field program MC3E and possibly from IPHEX will be considered for context.

Cecil, Daniel J.↗
NASA NTRS2004

Drilling to Extract Liquid Water on Mars: Feasible and Worth the Investment

A critical application for the success of the Exploration Mission is developing cost effective means to extract resources from the Moon and Mars needed to support human exploration. Water is the most important resource in this regard, providing a critical life support consumable, the starting product of energy rich propellants, energy storage media (e.g. fuel cells), and a reagent used in virtually all manufacturing processes. Water is adsorbed and chemically bound in Mars soils, ice is present near the Martian surface at high latitudes, and water vapor is a minor atmospheric constituent, but extracting meaningful quantities requires large complex mechanical systems, massive feedstock handling, and large energy inputs. Liquid water aquifers are almost certain to be found at a depth of several kilometers on Mars based on our understanding of the average subsurface thermal gradient, and geological evidence from recent Mars missions suggests liquid water may be present much closer to the surface at some locations. The discovery of hundreds of recent water-carved gullies on Mars indicates liquid water can be found at depths of 200-500 meters in many locations. Drilling to obtain liquid water via pumping is therefore feasible and could lower the cost and improve the return of Mars exploration more than any other ISRU technology on the horizon. On the Moon, water ice may be found in quantity in permanently shadowed regions near the poles.

Stoker, C.↗
NASA NTRS2004

The Airframe Noise Reduction Challenge

The NASA goal of reducing external aircraft noise by 10 dB in the near-term presents the acoustics community with an enormous challenge. This report identifies technologies with the greatest potential to reduce airframe noise. Acoustic and aerodynamic effects will be discussed, along with the likelihood of industry accepting and implementing the different technologies. We investigate the lower bound, defined as noise generated by an aircraft modified with a virtual retrofit capable of eliminating all noise associated with the high lift system and landing gear. However, the airframe noise of an aircraft in this 'clean' configuration would only be about 8 dB quieter on approach than current civil transports. To achieve the NASA goal of 10 dB noise reduction will require that additional noise sources be addressed. Research shows that energy in the turbulent boundary layer of a wing is scattered as it crosses trailing edge. Noise generated by scattering is the dominant noise mechanism on an aircraft flying in the clean configuration. Eliminating scattering would require changes to much of the aircraft, and practical reduction devices have yet to receive serious attention. Evidence suggests that to meet NASA goals in civil aviation noise reduction, we need to employ emerging technologies and improve landing procedures; modified landing patterns and zoning restrictions could help alleviate aircraft noise in communities close to airports.

Lockard, David P.↗
NASA NTRS1996

Mixing and Transition Control Studied

Considerable progress in understanding nonlinear phenomena in both unbounded and wallbounded shear flow transition has been made through the use of a combination of high- Reynolds-number asymptotic and numerical methods. The objective of this continuing work is to fully understand the nonlinear dynamics so that ultimately (1) an effective means of mixing and transition control can be developed and (2) the source terms in the aeroacoustic noise problem can be modeled more accurately. Two important aspects of the work are that (1) the disturbances evolve from strictly linear instability waves on weakly nonparallel mean flows so that the proper upstream conditions are applied in the nonlinear or wave-interaction streamwise region and (2) the asymptotic formulations lead to parabolic problems so that the question of proper out-flow boundary conditions--still a research issue for direct numerical simulations of convectively unstable shear flows--does not arise. Composite expansion techniques are used to obtain solutions that account for both mean-flow-evolution and nonlinear effects. A previously derived theory for the amplitude evolution of a two-dimensional instability wave in an incompressible mixing layer (which is in quantitative agreement with available experimental data for the first nonlinear saturation stage for a plane-jet shear layer, a circular-jet shear layer, and a mixing layer behind a splitter plate) have been extended to include a wave-interaction stage with a three-dimensional subharmonic. The ultimate wave interaction effects can either give rise to explosive growth or an equilibrium solution, both of which are intimately associated with the nonlinear self-interaction of the three dimensional component. The extended theory is being evaluated numerically. In contrast to the mixing-layer situation, earlier comparisons of theoretical predictions based on asymptotic methods and experiments in wall-bounded shear-flow transition have been somewhat lacking in one aspect or another. The current work strongly suggests that the main weakness is the underlying asymptotic representation of the linear "part" of the problem and not the explicit modeling of the nonlinear/wave-interaction effects. Consequently, the long-wave-length/high-Reynolds-number asymptotic limit for the Blasius boundary-layer stability problem was reexamined, and a new dispersion relationship for the instability waves that is uniformly valid for both the upper- and lower branch regions to the required order of approximation was obtained. A comparison with numerical results, obtained by solving the Orr-Sommerfeld stability problem, shows that the asymptotic formula provides surprisingly good results, even for values of the frequency parameter usually encountered in experimental investigations. This is particularly evident in the dynamically important upper-branch region, where much of the nonlinear interactions in transition experiments are believed to take place. The result is important in that it can be used to greatly improve the accuracy of weakly nonlinear critical-layer-based theories, and a consistent nonlinear theory is currently under evaluation.

Source record↗
NASA NTRS1991

Evidence of chaotic pattern in solar flux through a reproducible sequence of period-doubling-type bifurcations

Presented here is a preliminary study of the limits to solar flux intensity prediction, and of whether the general lack of predictability in the solar flux arises from the nonlinear chaotic nature of the Sun's physical activity. Statistical analysis of a chaotic signal can extract only its most gross features, and detailed physical models fail, since even the simplest equations of motion for a nonlinear system can exhibit chaotic behavior. A recent theory by Feigenbaum suggests that nonlinear systems that can be led into chaotic behavior through a sequence of period-doubling bifurcations will exhibit a universal behavior. As the control parameter is increased, the bifurcation points occur in such a way that a proper ratio of these will approach the universal Feigenbaum number. Experimental evidence supporting the applicability of the Feigenbaum scenario to solar flux data is sparse. However, given the hypothesis that the Sun's convection zones are similar to a Rayleigh-Bernard mechanism, we can learn a great deal from the remarkable agreement observed between the prediction by theory (period doubling - a universal route to chaos) and the amplitude decrease of the signal's regular subharmonics. The authors show that period-doubling-type bifurcation is a possible route to a chaotic pattern of solar flux that is distinguishable from the logarithm of its power spectral density. This conclusion is the first positive step toward a reformulation of solar flux by a nonlinear chaotic approach. The ultimate goal of this research is to be able to predict an estimate of the upper and lower bounds for solar flux within its predictable zones. Naturally, it is an important task to identify the time horizons beyond which predictability becomes incompatible with computability.

Ashrafi, S.↗
NASA NTRS1991

Evidence of chaotic pattern in solar flux through a reproducible sequence of period-doubling-type bifurcations

A preliminary study of the limits to solar flux intensity prediction, and of whether the general lack of predictability in the solar flux arises from the nonlinear chaotic nature of the Sun's physical activity is presented. Statistical analysis of a chaotic signal can extract only its most gross features, and detailed physical models fail, since even the simplest equations of motion for a nonlinear system can exhibit chaotic behavior. A recent theory by Feigenbaum suggests that nonlinear systems that can be led into chaotic behavior through a sequence of period-doubling bifurcations will exhibit a universal behavior. As the control parameter is increased, the bifurcation points occur in such a way that a proper ratio of these will approach the universal Feigenbaum number. Experimental evidence supporting the applicability of the Feigenbaum scenario to solar flux data is sparse. However, given the hypothesis that the Sun's convection zones are similar to a Rayleigh-Bernard mechanism, we can learn a great deal from the remarkable agreement observed between the prediction by theory (period doubling - a universal route to chaos) and the amplitude decrease of the signal's regular subharmonics. It is shown that period-doubling-type bifurcation is a possible route to a chaotic pattern of solar flux that is distinguishable from the logarithm of its power spectral density. This conclusion is the first positive step toward a reformulation of solar flux by a nonlinear chaotic approach. The ultimate goal of this research is to be able to predict an estimate of the upper and lower bounds for solar flux within its predictable zones. Naturally, it is an important task to identify the time horizons beyond which predictability becomes incompatible with computability.

Ashrafi, S.↗
NASA NTRS2016

Multispectral VNIR Observations by the Opportunity Rover Pancam of Multiple Episodes of Aqueous Alteration in Marathon Valley, Endeavour Crater, Mars

Since early 2015, the Mars Exploration Rover Opportunity has been exploring the break in the rim of Endeavour Crater dubbed Marathon Valley by the rover team. Marathon Valley was identified by orbital hyperspectral data from the MRO CRISM as having a relatively strong spectral feature in the 2.3 micrometer region indicative of an Mg or Fe-OH combination overtone absorption band indicative of smectite clay. Earlier in its mission, Opportunity examined the Matijevic Hill region on the more northerly Cape York crater rim segment and found evidence for smectite clays in a stratigraphically lower, pre-impact formed unit dubbed the Matijevic formation. However, the smectite exposures in Marathon Valley appear to be associated with the stratigraphically higher Shoemaker formation impact breccia. Evidence for alteration in this unit in Marathon Valley is provided by Pancam multispectral observations in the 430 to 1010 nm visible/near infrared (VNIR) spectral range. Sinuous troughs ("red zones") contain fragmented cobbles and pebbles displaying higher blue-to-red slopes, moderately higher 535 nm band depths, elevated 754 to 934 nm, and negative 934 to 1009 nm slopes. The lack of an absorption at 864 to 904 nm indicates the lack of crystalline red hematite in these red zones, but likely an enrichment in nanophase ferric oxides. The negative 934 to 1009 nm slope is potentially indicative of the presence of adsorbed or structurally bound water. A scuff in a red zone near the southern wall of Marathon Valley uncovered light-toned soils and a pebble with an 803 to 864 nm absorption resembling that of light-toned Fe-sulfate bearing soils uncovered by the Spirit rover in the Columbia Hills of Gusev crater. APXS chemical measurements indicated enrichments of Mg and S in the scuff soils and the pebble, Joseph Field, with the strongest 803 nm band- consistent with Mg and Fe sulfates. The presence of Fe and Mg sulfates can be interpreted as evidence of a potentially later episode of aqueous alteration with an earlier, neutral to alkaline pH episode forming the Fe/Mg smectites and a later acid pH episode forming the Fe and Mg sulfates.

Farrand, William H.↗
NASA NTRS1990

Sensitivity of liquid bridges subject to axial residual acceleration

It has become evident that the float zone crystal growth method and isothermal liquid bridges may be very sensitive to the residual acceleration environment of a spacelab. Using a slender-body approximation, the problem of determining the axisymmetric response of the shape of the free surface of a cylindrical liquid column bounded by two solid regions is modeled by a one-dimensional system of nonlinear equations. It is found that the sensitivity of the zone shape depends on the static Bond number, BO, aspect ratio, and viscosity, as well as the amplitude and frequency of the disturbance. The general trend is an increase in tolerable residual gravity with increasing frequency. At the eigenfrequencies of the zone, however, there are dramatic deviations from this trend. At these frequencies the tolerable residual gravity level can be two orders of magnitude lower at this frequency.

Zhang, Yiqiang↗
NASA NTRS2007

Aerosol Absorption and Radiative Forcing

We present a comprehensive examination of aerosol absorption with a focus on evaluating the sensitivity of the global distribution of aerosol absorption to key uncertainties in the process representation. For this purpose we extended the comprehensive aerosol-climate model ECHAM5-HAM by effective medium approximations for the calculation of aerosol effective refractive indices, updated black carbon refractive indices, new cloud radiative properties considering the effect of aerosol inclusions, as well as by modules for the calculation of long-wave aerosol radiative properties and instantaneous aerosol forcing. The evaluation of the simulated aerosol absorption optical depth with the AERONET sun-photometer network shows a good agreement in the large scale global patterns. On a regional basis it becomes evident that the update of the BC refractive indices to Bond and Bergstrom (2006) significantly improves the previous underestimation of the aerosol absorption optical depth. In the global annual-mean, absorption acts to reduce the shortwave anthropogenic aerosol top-of-atmosphere (TOA) radiative forcing clear-sky from -0.79 to -0.53 W m(sup -2) (33%) and all-sky from -0.47 to -0.13W m(sup -2 (72%). Our results confirm that basic assumptions about the BC refractive index play a key role for aerosol absorption and radiative forcing. The effect of the usage of more accurate effective medium approximations is comparably small. We demonstrate that the diversity in the AeroCom land-surface albedo fields contributes to the uncertainty in the simulated anthropogenic aerosol radiative forcings: the usage of an upper versus lower bound of the AeroCom land albedos introduces a global annual-mean TOA forcing range of 0.19W m(sup -2) (36%) clear-sky and of 0.12W m(sup -2) (92%) all-sky. The consideration of black carbon inclusions on cloud radiative properties results in a small global annual-mean all-sky absorption of 0.05W m(sup -2) and a positive TOA forcing perturbation of 0.02W m(sup -2). The long-wave aerosol radiative effects are small for anthropogenic aerosols but become of relevance for the larger natural dust and sea-salt aerosols.

Stier, Philip↗
NASA NTRS2003

Numerical Simulation of High Drag Reduction in a Turbulent Channel Flow with Polymer Additives

The addition of small amounts of long chain polymer molecules to wall-bounded flows can lead to dramatic drag reduction. Although this phenomenon has been known for about fifty years, the action of the polymers and its effect on turbulent structures are still unclear. Detailed experiments have characterized two distinct regimes (Warholic et al. 1999), which are referred to as low drag reduction (LDR) and high drag reduction (HDR). The first regime exhibits similar statistical trends as Newtonian flow: the log-law region of the mean velocity profile remains parallel to that of the Newtonian ow but its lower bound moves away from the wall and the upward shift of the log-region is a function of drag reduction, DR. Although streamwise fluctuations are increased and transverse ones are reduced, the shape of the rms velocity profiles is not qualitatively modified. At higher drag reductions, of the order of 40-50%, the ow enters the HDR regime for which the slope of the log-law is dramatically augmented and the Reynolds shear stress is small (Warholic et al. 1999; Ptasinski et al. 2001). The drag reduction is eventually bounded by a maximum drag reduction (MDR) (Virk & Mickley 1970) which is a function of the Reynolds number. While several experiments report mean velocity profiles very close to the empirical profile of Virk & Mickley (1970) for MDR conditions, the observations regarding the structure of turbulence can differ significantly. For instance, Warholic et al. (1999) measured a near-zero Reynolds shear stress, whereas a recent experiment (Ptasinski et al. 2001) shows evidence of non-negligible Reynolds stress in their MDR flow. To the knowledge of the authors, only the LDR regime has been documented in numerical simulations (Sureshkumar et al. 1997; Dimitropoulos et al. 1998; Min et al. 2001; Dubief & Lele 2001; Sibilla & Baron 2002). This paper discusses the simulation of polymer drag reduced channel ow at HDR using the FENE-P (Finite Elastic non-linear extensibility-Peterlin) model which was used for the first LDR simulation by Sureshkumar et al. (1997). Flow and polymer parameters are close to realistic polymer drag reducing conditions. High drag reductions are achieved by using finite differences and a robust time stepping technique. A minimal channel flow is also used as a numerical experiment to investigate the effect of the outer region turbulent structures on the overall drag at HDR. The drag reducing action of the model is finally studied through the structure of energy transfers from the polymers to the velocity components. This investigation sheds some light on the details of polymer drag reduction.

Dubief, Yves↗
NASA NTRS1986

Archean foreland basin tectonics in the Witwatersrand, South Africa

The Witwatersrand Basin of South Africa is the best-known of Archean sedimentary basins and contains some of the largest gold reserves in the world. Sediments in the basin include a lower flysch-type sequence and an upper molassic facies, both of which contain abundant silicic volcanic detritus. The strata are thicker and more proximal on the northwestern side of the basin which is, at least locally, bound by thrust faults. These features indicate that the Witwatersrand strata may have been deposited in a foreland basin and a regional geologic synthesis suggests that this basin developed initially on the cratonward side of an Andean-type arc. Remarkably similar Phanerozoic basins may be found in the southern Andes above zones of shallow subduction. It is suggested that the continental collision between the Kaapvaal and Zimbabwe Cratons at about 2.7 Ga caused further subsidence and deposition in the Witwatersrand Basin. Regional uplift during this later phase of development placed the basin on the cratonward edge of a collision-related plateau, now represented by the Limpopo Province. Similarities are seen between this phase of Witwatersrand Basin evolution and that of active basins north of the Tibetan Plateau. The geologic evidence does not agree with earlier suggestions that the Witwatersrand strata were deposited in a rift or half-graben.

Burke, K.↗
NASA NTRS1986

Archean foreland basin tectonics in the Witwatersrand, South Africa

The Witwatersrand Basin of South Africa is the best-known of Archean sedimentary basins and contains some of the largest gold reserves in the world. Sediments in the basin include a lower flysch-type sequence and an upper molassic facies, both of which contain abundant silicic volcanic detritus. The strata are thicker and more proximal on the northwestern side of the basin which is, at least locally, bound by thrust faults. These features indicate that the Witwatersrand strata may have been deposited in a foreland basin and a regional geologic synthesis suggests that this basin developed initially on the cratonward side of an Andean-type arc. Remarkably similar Phanerozoic basins may be found in the southern Andes above zones of shallow subduction. It is suggested that the continental collision between the Kaapvaal and Zimbabwe Cratons at about 2.7 Ga caused further subsidence and deposition in the Witwatersrand Basin. Regional uplift during this later phase of development placed the basin on the cratonward edge of a collision-related plateau, now represented by the Limpopo Province. Similarities are seen between this Phase of Witywatersrand Basin evolution and that of active basins north of the Tibetan Plateau. The geologic evidence does not agree with earlier suggestions that the Witwatersrand strata were deposited in a rift or half-graben.

Burke, K.↗
NASA NTRS2017

Biosignatures in the Context of Low Energy Flux

Many of the features that are thought of as biosignatures - including the mediation of chemical and physical processes with speed, specificity, and selectivity - result directly or indirectly from life's unique capability to mediate and direct energy flux. As such, it is important to consider the impact that differences in energy flux may have on the quantity and quality of evidence for life. Earth differs from every other body in our solar system in the magnitude of biologically-usable energy flux into a liquid water environment. On a global basis, the capture of light energy into photosynthesis and the flux of chemical energy represented in the products of that photosynthesis (organic material + O2) are about six and four orders of magnitude larger, respectively, than the flux of energy represented in geochemical sources. Our conception of what an inhabited world "looks like" and our intuition about how to search for life are based in this high-energy context. Energy fluxes on worlds beyond Earth may be better approximated by the million-fold smaller flux provided to Earth's biosphere by geochemical sources. As a result, the nature, abundance, and quality of evidence for life that could be expected on an inhabited extraterrestrial world within our solar system may differ profoundly from that found on Earth. Understanding this potential difference in quantitative terms provides important context for the formulation of life detection strategies. The influence of energy flux on biosignatures can be evaluated through reference to the two basic purposes into which life partitions energy flux: (1) Life expends energy to sustain existing biomass in a metabolic steady state (metabolically functional but non-growing). The formal representation of this relationship in the traditional microbiology literature equates biomass directly with energy flux. The direct implication is that worlds having lower energy flux will have correspondingly lower potential to support biomass. Life detection strategies that directly target extant organisms should therefore be prepared to encounter average biomass densities that may be many orders of magnitude smaller than those found in most of Earth's surface environments (2) Life expends energy to synthesize new biomass. An end-member case in which new biomass is created at the energy-limited rate and the corresponding cells are immediately destroyed (so that the energy partitioned to cell maintenance is minimized) establishes an upper bound on the rate at which biological material can enter a bulk global pool. For a specified bulk concentration [i] of any particular biological compound, i, or for biologically produced matter overall, this synthesis rate, R (sub i), defines a characteristic time scale tau (sub i) equals [i] divided by R (sub i). tau (sub i) can be thought of as (a) the minimum time required for biosynthesis to yield a specific bulk concentration (e.g., a detection threshold) of i, and (b) the average residence time of i within a bulk pool when [i] is held in steady state through a balance between biosynthesis and attrition by physical, chemical, or biological consumption. tau (sub i) becomes an important quantity in considering the potential utility of enantiomeric excess (as a product of homochiral biosynthesis) as a biosignature. Spontaneous racemization of amino acids acts to "erase" the signature of homochiral synthesis over time scales that may range from hundreds to hundreds of thousands of years, depending on temperature. For environments in which low energy flux translates to low rates of biosynthesis, including the synthesis of homochiral amino acids, amino acid residence times in pools having detectable concentrations may compare to or significantly exceed the time scale for racemization. This and similar consequences of long residence times should be considered in the formulation of life detection strategies based on detection of biologically-produced species. Fluxes of biologically-useful energy on potentially habitable worlds within our solar system are, at present, not well constrained. Improving such constraint has the potential to inform priorities in the formulation and targeting of search-for-life strategies, based on the implications of energy flux for the abundance and quality of biosignatures overall, and in specific categories.

ENERGY FLUX↗
NASA NTRS1997

A 70th Degree Lunar Gravity Model (GLGM-2) from Clementine and other tracking data

A spherical harmonic model of the lunar gravity field complete to degree and order 70 has been developed from S band Doppler tracking data from the Clementine mission, as well as historical tracking data from Lunar Orbiters 1-5 and the Apollo 15 and 16 subsatellites. The model combines 361,000 Doppler observations from Clementine with 347,000 historical observations. The historical data consist of mostly 60-s Doppler with a noise of 0.25 to several mm/s. The Clementine data consist of mostly 10-s Doppler data, with a data noise of 0.25 mm/s for the observations from the Deep Space Network, and 2.5 mm/s for the data from a naval tracking station at Pomonkey, Maryland. Observations provided Clementine, provide the strongest satellite constraint on the Moon's low-degree field. In contrast the historical data, collected by spacecraft that had lower periapsis altitudes, provide distributed regions of high-resolution coverage within +/- 29 deg of the nearside lunar equator. To obtain the solution for a high-degree field in the absence of a uniform distribution of observations, we applied an a priori power law constraint of the form 15 x 10(exp -5)/sq l which had the effect of limiting the gravitational power and noise at short wavelengths. Coefficients through degree and order 18 are not significantly affected by the constraint, and so the model permits geophysical analysis of effects of the major basins at degrees 10-12. The GLGM-2 model confirms major features of the lunar gravity field shown in previous gravitational field models but also reveals significantly more detail, particularly at intermediate wavelengths (10(exp 3) km). Free-air gravity anomaly maps derived from the new model show the nearside and farside highlands to be gravitationally smooth, reflecting a state of isostatic compensation. Mascon basins (including Imbrium, Serenitatis, Crisium, Smythii, and Humorum) are denoted by gravity highs first recognized from Lunar Orbiter tracking. All of the major mascons are bounded by annuli of negative anomalies representing significant subsurface mass deficiencies. Mare Orientale appears as a minor mascon surrounded by a horseshoe-shaped gravity low centered on the Inner and Outer Rook rings that is evidence of significant subsurface structural heterogeneity. Although direct tracking is not available over a significant part of the lunar farside, GLGM-2 resolves negative anomalies that correlate with many farside basins, including South Pole-Aitken, Hertzsprung, Korolev, Moscoviense, Tsiolkovsky, and Freundlich-Sharonov.

Lemonie, Frank G. R.↗
NASA NTRS1998

Effects of Convective Solute and Impurity Transport in Protein Crystal Growth

High-resolution optical interferometry was used to investigate the effects of forced solution convection on the crystal growth kinetics of the model protein lysozyme. Most experiments were conducted with 99.99% pure protein solutions. To study impurity effects, approx. 1% of lysozyme dimer (covalently bound) was added in some cases. We show that the unsteady kinetics, corresponding to bunching of growth steps, can be characterized by the Fourier components of time traces of the growth rate. Specific Fourier spectra are uniquely determined by the solution conditions (composition, temperature, and flow rate) and the growth layer source activity. We found that the average step velocity and growth rate increase by approx. I0% with increasing flow rate, as a result of the enhanced solute supply to the interface. More importantly, faster convective transport results in lower fluctuation amplitudes. This observation supports our rationale for system-dependent effects of transport on the structural perfection of protein crystals. We also found that solution flow rates greater than 500 microns/s result in stronger fluctuations while the average growth rate is decreased. This can lead to growth cessation at low supersaturations. With the intentionally contaminated solutions, these undesirable phenomena occurred at about half the flow rates required in pure solutions. Thus, we conclude that they are due to enhanced convective supply of impurities that are incorporated into the crystal during growth. Furthermore, we found that the impurity effects are reduced at higher crystal growth rates. Since the exposure time of terraces is inversely proportional to the growth rate, this observation suggests that the increased kinetics instability results from impurity adsorption on the interface. Finally, we provide evidence relating earlier observations of "slow protein crystal growth kinetics" to step bunch formation in response to nonsteady step generation.

Vekilov, Peter G.↗
NASA NTRS1999

Fluorescence Studies of Protein Crystal Nucleation

Fluorescence can be used to study protein crystal nucleation through methods such as anisotropy, quenching, and resonance energy transfer (FRET), to follow pH and ionic strength changes, and follow events occurring at the growth interface. We have postulated, based upon a range of experimental evidence that the growth unit of tetragonal hen egg white lysozyme is an octamer. Several fluorescent derivatives of chicken egg white lysozyme have been prepared. The fluorescent probes lucifer yellow (LY), cascade blue, and 5-((2-aminoethyl)aminonapthalene-1-sulfonic acid (EDANS), have been covalently attached to ASP 101. All crystallize in the characteristic tetragonal form, indicating that the bound probes are likely laying within the active site cleft. Crystals of the LY and EDANS derivatives have been found to diffract to at least 1.7 A. A second group of derivatives is to the N-terminal amine group, and these do not crystallize as this site is part of the contact region between the adjacent 43 helix chains. However derivatives at these sites would not interfere with formation of the 43 helices in solution. Preliminary FRET studies have been carried out using N-terminal bound pyrene acetic acid (Ex 340 nm, Em 376 nm) lysozyme as a donor and LY (Ex -425 nm, Em 525 nm) labeled lysozyme as an acceptor. FRET data have been obtained at pH 4.6, 0.1 M NaAc buffer, at 5 and 7% NaCl, 4 C. The corresponding Csat values are 0.471 and 0.362 mg/ml (approximately 3.3 and approximately 2.5 x 10(exp -5) M respectively). The data at both salt concentrations show a consistent trend of decreasing fluorescence intensity of the donor species (PAA) with increasing total protein concentration. This decrease is more pronounced at 7% NaCl, consistent with the expected increased intermolecular interactions at higher salt concentrations reflected in the lower solubility. The calculated average distance between any two protein molecules at 5 x 10(exp -6) M is approximately 70nm, well beyond the range where any FRET can be expected. Results from these and ongoing studies will be presented.

Pusey, Marc L.↗
NASA NTRSDate not supplied

How Can We Efficiently Build A Spacecraft That Has Longevity? Experiences From the GSFC Perspective to Inform Lunar Exploration and Science Orbiter’s Architecture

Recent successes in NASA planetary science missions has shown that long-lived missions can yield significant science return. These missions, despite their longevity, were not planned to operate beyond their deign life. For example, the Lunar Reconnaissance Orbiter has a design life of 2-3 years, and yet we are entering its 14th year on orbit. Spacecraft longevity in practice has been related to: 1) mission class; 2) did we test it long enough and resolve all the anomalies to be beyond the early failure curve; 3) consumables budgets, and 4) how components are de-signed and tested. Mission class has been perceived as one of the primary ways to drive longevity. But higher mission class is a significant cost and mission driver. Parts selection only from the limited military standard parts and extensive parts qualification adds to development time and cost. Largely redundant (often erroneously interpreted as fully redundant) adds to launch mass and increases testing complexity (which may reduce the amount of testing in the nominal con-figuration). Lower Risk Posture (reflected in a higher mission class) drives significant additional processes and quality assurance analyses. Higher mission class also drives significantly greater sparing and life testing costs. This discussion focuses on whether this is indeed the best way to achieve longevity efficiently or whether there are more efficient ways to achieve longevity. Empirical evidence shows that lower mission classes that implement effective risk reduction and selective redundancy generally results in long life at a lower Spacecraft bus cost. By evaluating redundancy careful-ly, spacecraft cost can be lowered which can enable a more capable payload. Risk of the mission is highly dependent on the complexity of the mission and is only loosely dependent on Class of Mission. High complexity missions can have many single point failures and require the development of new technology, while lower class mission can have lower risk by baselining or incorporating: (selective) redundancy, fault-tolerant design, design for minimum risk, ability to reset, and/or design for graceful degradation. The team met with Goddard Space Flight Center Space Systems Mission Operations leaders to discuss which avionics have proven in flight to be the most reliable and which have had lifetime issues. The paper proposes a list of components to focus on for redundancy for a long-lived lunar mission. Reliability numbers are presented for both single string and dual string missions. The history of life-time performance versus planned mission life according to mission class is presented. A mission with well thought out selective redundancy and effective on the ground test program, can be expected to last well beyond its mission lifetime and provided enhanced return for the community. This approach minimizes project expenditures that do not retire significant risk and allows the project to focus risk mitigation efforts on those risks that will have a significant likelihood of threatening mission success. This is aided by keeping the amount of technology maturation and mission complexity low, while focusing effort on ensuring all component stress-ing parameters well within the bounds of their capabilities.

Lessons Learned↗
NASA NTRS2000

Fluorescence Studies of Protein Crystal Nucleation

We have postulated that, in the case of tetragonal chicken egg white lysozyme, crystal growth occurs by the addition of pre-critical nuclei sized n-mers that form in the bulk solution, and that the n-mer growth units were multiples of the tetrameric 4(sub 3) helical structure. These have the strongest intermolecular bonds in the crystal and are therefore likely to be the first species formed. High resolution AFM studies provide strong supporting evidence for this model, but the data also suggest that the actual species in solution may not be identical in structure to that found in the crystal. We are using fluorescence resonance energy transfer (FRET) to study the initial solution phase self-assembly process, using covalent fluorescent derivatives which crystallize in the characteristic P4(sub 3)2(sub 1)2(sub 1) space group. FRET studies are being carried out between the cascade blue (CB-lys, donor, Ex(sub max) 366 nm, Em 420 nm) and lucifer yellow (LY-lys, acceptor, Ex(sub max) 430 nm, Em 528 nm) asp101 derivatives. The estimated R(sub 0) for this probe pair, the distance where 50% of the donor energy is transferred to the acceptor, is approx. 1.2 nm, compared to 2.2 nm between the side chain carboxyls of adjacent asp101's in the crystalline 4(sub 3) helix. The short donor lifetime of 2.80 ns (chi(sup 2) = 0.644), coupled with the large average distances between the molecules (greater than or equal to 50 nm) in solution, ensure that any energy transfer observed is not due to random diffusive interactions. Lifetime data show that CB-lys has a single lifetime when it is the only species in solution. Similarly, LY-lys also exhibits a single lifetime of 4.63 ns (chi(sup 2) = 0.42) when alone in solution. Addition of LY-lys to CB-lys results in the appearance of a third lifetime component of 0.348ns for the CB-lys. The fractional intensities of the different species present can be used to estimate the distribution of monomer and n-mers in solution. The self-association process is a function of the protein concentration relative to the saturation concentration, and observing it in dilute solution (conc. less than or equal to 10(exp -5)M) requires that the experiments be performed under low solubility conditions, i.e., low temperatures and high salt concentrations. Data from preliminary steady state FRET studies with N-terminal bound pyrene acetic acid (PAA-lys, donor, Ex 340 nm, Em 376 nm) and asp101 LY-lys as an acceptor showed a consistent trend of decreasing donor fluorescence intensity with increasing total protein concentration. The FRET data have been obtained at pH 4.6, 0.1M NaAc buffer, at 5 and 7% NaCl, 4 C. The corresponding C(sub sat) values are 0.471 and 0.362 mg/ml (approx. 3.3 and approx. 2.5 x 10(exp -5)M respectively). The donor fluorescence decrease is more pronounced at7% NaCl, consistent with the expected increased intermolecular interactions at higher salt concentrations as reflected in the lower solubility. Results from these and other ongoing studies will be discussed in conjunction with an emerging model for how tetragonal lysozyme crystals nucleate and the relevance of that model to other proteins.

Pusey, Marc↗