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Microbial contamination of spacecraft

Spacecraft and space habitats supporting human exploration contain a diverse population of microorganisms. Microorganisms may threaten human habitation in many ways that directly or indirectly impact the health, safety, or performance of astronauts. The ability to produce and maintain spacecraft and space stations with environments suitable for human habitation has been established over 40 years of human space flight. An extensive database of environmental microbiological parameters has been provided for short-term (< 20 days) space flight by more than 100 missions aboard the Space Shuttle. The NASA Mir Program provided similar data for long-duration missions. Interestingly, the major bacterial and fungal species found in the Space Shuttle are similar to those encountered in the nearly 15-year-old Mir. Lessons learned from both the US and Russian space programs have been incorporated into the habitability plan for the International Space Station. The focus is on preventive measures developed for spacecraft, cargo, and crews. On-orbit regular housekeeping practices complete with visual inspections are essential, along with microbiological monitoring. Risks associated with extended stays on the Moon or a Mars exploration mission will be much greater than previous experiences because of additional unknown variables. The current knowledge base is insufficient for exploration missions, and research is essential to understand the effects of space flight on biological functions and population dynamics of microorganisms in spacecraft. Equally important is a better understanding of the immune response and of human-microorganism-environment interactions during long-term space habitation.

Mir Project↗

Sensor for Monitoring Nanodevice-Fabrication Plasmas

The term plasma process diagnostics (PPD) refers to a spectroscopic technique and sensing hardware that have been proposed for monitoring plasma processes used to fabricate electronic devices that feature sizes as small as several nanometers. Nanometer dimensions are characteristic of the quantum level of miniaturization, where single impurity atoms or molecules can drastically change the local properties of the nanostructures. Such changes may be purposely used in nanoscale design but may also be extremely damaging or cause improper operation of the fabricated devices. Determination of temperature and densities of reactants near the developing features is important, since the structural synthesis is affected by characteristics of the local microenvironment. Consequently, sensors capable of nonintrusive monitoring with high sensitivity and high resolution are essential for real-time atomistic control of reaction kinetics and minimizing trace contamination in plasma processes used to fabricate electronic nanodevices. Such process-monitoring sensors are required to be compact, multiparametric, and immune to the harsh environments of processing plasmas. PPD is intended to satisfy these requirements. The specific technique used to implement plasma diagnostics with a PPD sensor would be an advanced version of continuous-wave cavity-ringdown spectroscopy (CW-CRDS) capable of profiling spectral line broadenings in order to derive both Doppler and Stark components. CRDS is based on measurements of the rate of absorption of laser light in an optical resonator. The ultimate sensitivity results from a very long absorption path length within the cavity and immunity to variations in incident laser intensity. The proposed version of this technique would involve the use of multiplexing tunable laser diodes and an actively modulated high-reflectivity optical resonator, thus offering a synergistic combination of simplicity, compactness, high sensitivity, and high resolution. The multiplexing capabilities of diode lasers could be utilized to make the PPD sensor a single, simple, compact, and inexpensive tool for the acquisition of multiparametric data. A PPD sensor would be capable of continuous measurement of such physical parameters as gas temperature, gas velocity, electron number density, and absolute densities of reacting chemical species. A laser beam can be easily adjusted to analyze the immediate vicinity of the growing nanostructures (or features etched down) in real time. The absorption enhancement in an optical cavity would afford the sensitivity needed for measurement of the temperature and densities of species at concentrations significantly lower than measurable by other nonintrusive techniques. It is anticipated that fully developed PPD sensors would enable simultaneous measurement of local temperature and determination of plasma species responsible for the synthesis and functionalization of nanodevices. These sensors would also enable tracking the pathways and origins of damaging contaminants, thereby providing feedback for adjustment of processes to optimize them and reduce contamination. The PPD sensors should also be useful for optimization of conventional microelectronics manufacturing plasma processes. Going beyond plasma processes for fabrication of electronic devices, PPD sensors could be used for monitoring of atoms, molecules, ions, radicals, clusters, and particles in a variety of other settings, including outer space. Because of their high sensitivity, such sensors could also prove useful for detecting traces of illegal drugs and explosives.

Bolshakov, Alexander↗

Spacecraft Charging Considerations and Design Efforts for the Orion Crew Module

The Orion Crew Module (CM) is nearing completion for the next flight, designated as Exploration Mission 1 (EM-1). For the uncrewed mission, the flight path will take the CM through a Perigee Raise Maneuver (PRM) out to an altitude of approximately 1800 km, followed by a Trans-Lunar Injection burn, a pass through the Van Allen belts then out to the moon for a lunar flyby, a Distant Retrograde Insertion (DRI) burn, a Distant Retrograde Orbit (DRO), a Distant Retrograde Departure (DRD) burn, a second lunar flyby, an Earth Insertion (EI) burn, and finally entry and landing. All of this, with the exception of the DRO associated maneuvers, is similar to the previous Apollo 8 mission in late 1968. In recent discussions, it is now possible that EM-1 will be a crewed mission, and if this happens, the orbit may be quite different from that just described. In this case, the flight path may take the CM on an out and back pass through the Van Allen belts twice, then out to the moon, again passing through the Van Allen belts twice, then finally back home. Even if the current EM-1 mission doesn't end up as a crewed mission, EM-2 and subsequent missions will undoubtedly follow orbital trajectories that offer comparable exposures to heightened vehicle charging effects. Because of this, and regardless of flight path, the CM vehicle will likely experience a wide range of exposures to energetic ions and electrons, essentially covering the gamut between low earth orbit to geosynchronous orbit and beyond. National Aeronautical and Space Administration (NASA) and Lockheed Martin (LM) engineers and scientists have been working to fully understand and characterize the vehicle's immunity level with regard to surface and deep dielectric charging, and the ramifications of that immunity level pertaining to materials and impacts to operational avionics, communications, and navigational systems. This presentation attempts to chronicle these efforts in a summary fashion, and attempts to capture the results of that work as they pertain to the electrical and avionic systems on-board the Orion CM.

Scully, Bob↗

Multiplexed Force and Deflection Sensing Shell Membranes for Robotic Manipulators

Force sensing is an essential requirement for dexterous robot manipulation, e.g., for extravehicular robots making vehicle repairs. Although strain gauges have been widely used, a new sensing approach is desirable for applications that require greater robustness, design flexibility including a high degree of multiplexibility, and immunity to electromagnetic noise. This invention is a force and deflection sensor a flexible shell formed with an elastomer having passageways formed by apertures in the shell, with an optical fiber having one or more Bragg gratings positioned in the passageways for the measurement of force and deflection.

Park, Yong-Lae↗

Overview of NASA research in fiber optics for aircraft controls

The challenge of those involved in aircraft control system hardware development is to accommodate an ever-increasing complexity in aircraft control, while limiting the size and weight of the components and improving system reliability. A technology that displays promise towards this end is fiber optics. The primary advantages of employing optical fibers, passive optical sensors and optically controlled actuators are weight/volume reduction, immunity from electromagnetic effects, high bandwidth capabilities and freedom from short circuits/sparking contacts. Since 1975, NASA Lewis has been performing in-house, contract and grant research in fiber optic sensors, high temperature electro-optic switches and fly-by-light control system architecture. Passive optical sensor development is an essential yet challenging area of work and has therefore received much attention during this period. A major effort to develop fly-by-light control system technology, known as the Fiber Optic Control System Integration (FOCSI) program, was initiated in 1985 as a cooperative effort between NASA and DOD. Phase 1 of FOCSI, completed in 1986, was aimed at the design of a fiber optic integrated propulsion/flight control system. Phase 2 will provide subcomponent and system development and system testing. In addition to a summary of the benefits of fiber optics, the FOCSI program, sensor advances, and future directions in the NASA Lewis program are discussed.

Seng, Gary T.↗

Insulin secretion and sensitivity in space flight: diabetogenic effects

Nearly three decades of space flight research have suggested that there are subclinical diabetogenic changes that occur in microgravity. Alterations in insulin secretion, insulin sensitivity, glucose tolerance, and metabolism of protein and amino acids support the hypothesis that insulin plays an essential role in the maintenance of muscle mass in extended-duration space flight. Experiments in flight and after flight and ground-based bedrest studies have associated microgravity and its experimental paradigms with manifestations similar to those of diabetes, physical inactivity, and aging. We propose that these manifestations are characterized best by an etiology that falls into the clinical category of "other" causes of diabetes, including, but not restricted to, genetic beta-cell defects, insulin action defects, diseases of the endocrine pancreas, endocrinopathies, drug or chemically induced diabetes, infections, immune-mediated metabolic alteration, and a host of genetic related diseases. We present data showing alterations in tumor necrosis factor-alpha production, insulin secretion, and amino acid metabolism in pancreatic islets of Langerhans cultured in a ground-based cell culture bioreactor that mimics some of the effects of microgravity. Taken together, space flight research, ground-based studies, and bioreactor studies of pancreatic islets of Langerhans support the hypothesis that the pancreas is unable to overcome peripheral insulin resistance and amino acid dysregulation during space flight. We propose that measures of insulin secretion and insulin action will be necessary to design effective countermeasures against muscle loss, and we advance the "disposition index" as an essential model to be used in the clinical management of space flight-induced muscle loss.

Review↗

ISS Payload Operations Training During the COVID-19 Pandemic: Impacts and Solutions

The onset of the COVID-19 pandemic brought a dramatic and rapid transformation to almost every aspect of humanity in 2020. The world’s space agencies and their missions were not immune to the wide-sweeping changes. One discipline principally affected was mission operations and the various groups supporting that function. Mission support teams, especially for complex and crewed missions like the International Space Station were forced to rapidly rethink how and where control center staff performed their vital work. Operations training – an essential element to mission ops, had unique hurdles to overcome. Operations training is responsible for preparing astronaut crews for their missions, providing training to flight controllers, as well as ensuring that new team members are ready to join their colleagues. Every element of training was impacted by COVID restrictions. From orientation and introductory classes for new controllers, simulations and advanced lessons, On the Job Training and final evaluations; all aspects faced new challenges. Trainers at NASA’s Marshall Space Flight Center in Huntsville, Alabama, were forced to become more efficient with trainees and resources to continue supporting payload operations. The pandemic started in the USA in March 2020. Immediately, NASA mandated that the support for ISS real-time operations was critical. As a result, physical access to key facilities was restricted. Trainers and trainees had to quickly shift to 100 percent remote learning. In the short term, this was not a problem. However, instructors discovered lessons that they were accustomed to delivering in a classroom environment did not translate to remote teaching. Another hurdle to operations training was the mandate that all simulations could only be held remotely. The logistics of even small simulations proved to be challenging due to IT restrictions and public internet limitations. With simulations essentially stopped, as well as the restrictions on most OJT, trainees were essentially stopped in their advancement towards certification. Once limitations were identified, trainers prioritized new options. Transitioning to all electronic learning materials was a relatively easy fix. Teaching to large groups took additional shifts in the training paradigm. Methods for preparing astronauts for their missions were revised. Simulation supervisors found more efficient techniques to provide realistic training experience. Communication and approvals from management was essential. In every case, the payload operations instructors found novel solutions to all functions listed. This paper will discuss the factors and solutions payloads operations trainers found to keep scientific research on the ISS flying forward to mission success.

ISS↗

ISS Payload Operations Training Throughout the COVID-19 Pandemic: Impacts, Opportunities and Solutions

The onset of the COVID-19 pandemic brought a dramatic and rapid transformation to almost every aspect of humanity. The world’s space agencies and their missions were not immune to the wide-sweeping changes. One discipline principally affected was mission operations and the various groups supporting that function. Mission support teams, especially for complex and crewed missions like the International Space Station (ISS) were forced to rethink how and where control center staff performed their vital work. Operations training – an essential element to mission ops, had unique hurdles to overcome. Operations training is responsible for preparing astronaut crews for their missions, training and certifying flight controllers, as well as ensuring that new team members are ready to join their colleagues. Every element of training was impacted during the pandemic. From orientation and introductory classes for new controllers, simulations, and advanced lessons, On the Job Training (OJT) and final evaluations; all aspects faced challenges. Trainers at NASA’s Marshall Space Flight Center in Huntsville, Alabama were forced to become more efficient with trainees and resources to continue supporting ISS payload operations. The pandemic arrived in the USA in March 2020. Immediately, NASA mandated that the support for ISS real-time operations was critical. As a result, physical access to key facilities was restricted. Trainers and trainees had to quickly shift to 100% remote learning. In the short term, this was not a problem. However, instructors discovered lessons they were accustomed to delivering in a classroom environment often did not translate to remote teaching. Another hurdle to operations training was the mandate that all simulations could only be held remotely. The logistics of even small simulations proved to be challenging due to Information Technology (IT) restrictions and public internet limitations. With simulations essentially halted, as well as the restrictions on most OJT, trainees were essentially stopped in their advancement towards certification. Once limitations were identified, trainers prioritized new options. Transitioning to all electronic learning materials was a relatively easy fix. Teaching to large groups took additional shifts in the training paradigm. Methods for preparing astronauts for their missions were revised. Simulation supervisors found efficient techniques to provide realistic training experiences. Communication and coordination with management was essential. In every case, the payload operations instructors found novel solutions to all functions listed. This paper discusses the factors and solutions payloads operations trainers found to keep scientific research on the ISS flying forward to mission success.

ISS↗

Fiber optics for controls

The challenge of those involved in control-system hardware development is to accommodate an ever-increasing complexity in aircraft control, while limiting the size and weight of the components and improving system reliability. A technology that displays promise towards this end is the area of fiber optics for controls. The primary advantages of employing optical fibers, passive optical sensors, and optically controlled actuators are weight and volume reduction, immunity from electromagnetic effects, superior bandwidth capabilities, and freedom from short circuits and sparking contacts. Since 1975, NASA Lewis has performed in-house, contract, and grant research in fiber optic sensors, high-temperature electro-optic switches, and fly-by-light control-system architecture. Passive optical sensor development is an essential yet challenging area of work and has therefore received much attention during this period. A major effort to develop fly-by-light control-system technology, known as the Fiber-Optic Control System Integration (FOCSI) program, was initiated in 1985 as a cooperative effort between NASA and DOD. Phase 1 of FOCSI, completed in 1986, was aimed at the design of a fiber-optic integrated propulsion/flight control system. Phase 2, yet to be initiated, will provide subcomponent and system development, and a system engine test. In addition to a summary of the benefits of fiber optics, the FOCSI program, sensor advances, and future directions in the NASA Lewis program will be discussed.

Seng, Gary T.↗

ISS Payload Operations Training During the COVID-19 Pandemic: Impacts and Solutions

Introduction: The onset and the protracted duration of the COVID-19 pandemic brought a dramatic and rapid transformation to almost every aspect of humanity in 2020. The world’s space agencies and their missions were not immune to the unpredictable and wide-sweeping changes. One discipline principally affected was mission operations and the various groups supporting that critical function. Mission support teams, especially for complex and crewed missions like the International Space Station (ISS) were forced to rapidly rethink how and where control center staff performed their vital work. Operations training – an essential element to mission ops, had unique hurdles to overcome. Operations training is responsible for preparing astronaut crews for their scientific missions, providing recurring proficiency and currency material to flight controllers, as well as ensuring new team members are ready to join their colleagues on console. Every element of training was impacted by COVID restrictions. From orientation and introductory classes for new controllers, training for scientists and payload developers, to simulations and advanced lessons, critical On the Job Training (OJT) and final evaluations; all aspects faced new challenges. Operations trainers at NASA’s Marshall Space Flight Center (MSFC) in Huntsville, Alabama, were forced to rethink, retool, and become more efficient with trainees and training resources in order to continue supporting payload operations on the ISS. Impacts and Barriers to Operations Training: The pandemic started in the United States in early March 2020. Immediately, NASA mandated that the support for ISS real-time operations was critical. As a result, physical access to the ISS Payload Operations Center was restricted to only those people supporting console operations. Thus, use of on-site NASA facilities and any trainer to trainee face-to-face interaction was not authorized. Trainers and trainees had to quickly shift to 100% remote learning. In the short term, this was not a problem since NASA had already prepared Information Technology (IT) resources that facilitated remote access to mission resources like classes, documents and teleconferences. However, instructors quickly discovered the classes that they were accustomed to delivering in a large classroom environment did not translate well to remote teaching. The lack of non-verbal communication with the class became a major barrier to instruction. Another significant hurdle to operations training was the mandate that all simulations could only be held remotely. The logistics of planning and executing even small simulations proved to be challenging, almost impossible, due to IT technology and public internet limitations. With simulations essentially stopped, as well as the restrictions on most OJT training, trainees were essentially stopped in their advancement towards certification. An additional barrier that had to be overcome was the formal documentation of the curriculum objectives. Even though NASA had been deliberately transitioning to electronic documents for years, some organizations were still relying on “pen and ink” documents to formalize training completion. The logistics of implementing secure and controlled e-signature documents in a standardized, NASA approved format had to be addressed. Lesson Learned and Efficiencies: Once the various limitations were identified, ISS trainers at MSFC discussed, proposed, and prioritized new options and techniques. Transitioning to all electronic learning materials and certifications was a relevantly easy fix. Teaching courses to large and diverse groups took additional shifts in the NASA training paradigm. Likewise, methods and procedures for preparing astronauts for their missions were forced to be revised. Simulation supervisors and their teams were also forced to find more efficient techniques to provide realistic training experience. Communication and approvals from management was essential. In every case, the ISS payload operations instructors found novel and efficient solutions to all functions listed above. This paper will discuss the factors and solutions payloads operations trainers found to keep scientific research on the ISS flying forward to mission success.

ISS Operations↗

Shield to Pin Coupling of Lightning-Like Transients on Payload Umbilical Cables on a Launch Pad

In this paper we describe in-situ testing of a long payload umbilical, on a launch site, injected with “lightning- like” transients and describe resulting pin-to-pin voltages. Injections and voltage measurements near the ground support equipment room, as well as at a location near the payload junction box, are made. The umbilical cables tested include an outer over-braid and the inner conductor coupling is examined for open circuit, short-circuit and various loads representative of spacecraft input impedances. This testing is important because the Kennedy Space Center (KSC) where the lightning occurrence is the highest in the United States, is the primary launch site for Launch Services Program spacecraft customers. Lightning planning is essential but developing a lightning plan is often overlooked or not adequately analyzed leaving the spacecraft vulnerable to time delays or even damage when lightning occurs. At other popular launch sites like Vandenberg Air Force Base (VAFB) where lightning occurs less often, although at the same or greater intensity when it does occur, lightning planning is often completely ignored by the spacecraft. The two major questions to be addressed in the lightning plan are what retesting should be done to establish a “goodness” level and what is the trigger criteria for this testing? The spacecraft will typically use a standard spacecraft check-out procedure to address the necessary retesting, but determining the trigger criteria is often an issue. For instance, a spacecraft needs to understand what their immunity is to a certain lightning magnitude and location. Determining the amount of current that can be coupled onto a spacecraft umbilical can be calculated by using worst case assumptions or measured with current probes and current measurement devices. Spacecraft can also determine what pin-to-pin voltages they are sensitive to, however pin-to-pin voltage measurements are not typically taken during the strike due to the invasive nature of this measurement. In this paper, we present detailed data on the shield to pin voltage transfer functions to provide insight to the spacecraft developers for lightning retest criteria planning. The results from this unique testing opportunity provide essential details on specific coupling mechanisms affecting spacecraft hardware that interfaces with the ground support equipment. This missing link between cable shield currents and payload susceptibility voltages has been methodically tested and representative data presented.

Trout, Dawn↗

Slow Wave Sleep and Long Duration Spaceflight

While ground research has clearly shown that preserving adequate quantities of sleep is essential for optimal health and performance, changes in the progression, order and /or duration of specific stages of sleep is also associated with deleterious outcomes. As seen in Figure 1, in healthy individuals, REM and Non-REM sleep alternate cyclically, with stages of Non-REM sleep structured chronologically. In the early parts of the night, for instance, Non-REM stages 3 and 4 (Slow Wave Sleep, or SWS) last longer while REM sleep spans shorter; as night progresses, the length of SWS is reduced as REM sleep lengthens. This process allows for SWS to establish precedence , with increases in SWS seen when recovering from sleep deprivation. SWS is indeed regarded as the most restorative portion of sleep. During SWS, physiological activities such as hormone secretion, muscle recovery, and immune responses are underway, while neurological processes required for long term learning and memory consolidation, also occur. The structure and duration of specific sleep stages may vary independent of total sleep duration, and changes in the structure and duration have been shown to be associated with deleterious outcomes. Individuals with narcolepsy enter sleep through REM as opposed to stage 1 of NREM. Disrupting slow wave sleep for several consecutive nights without reducing total sleep duration or sleep efficiency is associated with decreased pain threshold, increased discomfort, fatigue, and the inflammatory flare response in skin. Depression has been shown to be associated with a reduction of slow wave sleep and increased REM sleep. Given research that shows deleterious outcomes are associated with changes in sleep structure, it is essential to characterize and mitigate not only total sleep duration, but also changes in sleep stages.

Whitmire, Alexandra↗

Production of Magnetic Turbulence by Cosmic Rays Drifting Upstream of Supernova Remnant Shocks

I will present results of our recent two- and three-dimensional Particle-In-Cell simulations of magnetic-turbulence production by cosmic-ray ions drifting upstream of supernova remnant shocks. These studies' aim is twofold: test recent predictions of strong amplification in short wavelength, non-resonant wave modes, and study the subsequent evolution of the magnetic turbulence, including its backreaction on cosmic-ray trajectories. We confirm that the drifting cosmic rays give rise to a turbulent magnetic field, but show that an oblique filamentary mode grows more rapidly than the non-resonant parallel modes found in analytical theory. The field perturbations grow more slowly than estimated using a quasi-linear analytical approach for the parallel plane-wave mode, and saturate in amplitude at deltaB/B approximately equal to 1. The backreaction of the magnetic turbulence on the particles leads to an alignment of the bulk-flow velocities of the cosmic rays and the background medium. This is an essential characteristic of cosmic ray-modified shocks: the upstream flow speed is continuously changed by the cosmic rays. The reduction of relative drift between cosmic rays and background medium accounts for the saturation of the instability at only moderate magnetic-field amplitudes. It is possible that the prolonged magnetic field growth observed in recent MHD simulations results from a cosmic-ray current assumed to be constant and thus immune to the backreaction from the turbulent field. We speculate that the parallel plane-wave mode found in analytical treatments very quickly leads co filamentation, which we observe in our PIC modeling and is also apparent in the MHD simulations.

Stroman, Thomas↗

Two Fiber Optical Fiber Thermometry

An optical fiber thermometer consists of an optical fiber whose sensing tip is given a metallic coating. The sensing tip of the fiber is essentially an isothermal cavity, so the emission from this cavity will be approximately equal to the emission from a blackbody. Temperature readings are obtained by measuring the spectral radiative heat flux at the end of the fiber at two wavelengths. The ratio of these measurements and Planck's Law are used to infer the temperature at the sensing tip. Optical fiber thermometers have high accuracy, excellent long-term stability and are immune to electromagnetic interference. In addition, they can be operated for extended periods without requiring re-calibration. For these reasons. it is desirable to use optical fiber thermometers in environments such as the International Space Station. However, it has recently been shown that temperature readings are corrupted by emission from the fiber when extended portions of the probe are exposed to elevated temperatures. This paper will describe several ways in which the reading from a second fiber can be used to correct the corrupted temperature measurements. The accuracy and sensitivity to measurement uncertainty will be presented for each method.

Jones, Mathew R.↗

Crystallization and preliminary X-ray analysis of Der f 2, a potent allergen derived from the house dust mite (Dermatophagoides farinae)

Although a number of allergens have been identified and isolated, the underlying molecular basis for the potent immune response is poorly understood. House dust mites (Dermatophagoides sp.) are ubiquitous contributors to atopy in developed countries. The rhinitis, dermatitis and asthma associated with allergic reactions to these arthropods are frequently caused by relatively small (125-129 amino acids) mite proteins of unknown biological function. Der f 2, a major allergen from the mite D. farinae, has been recombinantly expressed, characterized and crystallized. The crystals belong to the tetragonal space group I4(1)22, with unit-cell parameters a = b = 95.2, c = 103.3 A. An essentially complete (97.2%) data set has been collected to 2.4 A at a synchrotron source. Attempts to solve the crystal structure of Der f 2 by molecular replacement using the NMR coordinates for either Der f 2 or Der p 2 (the homologous protein from D. pteronyssinus) failed, but preliminary searches using the crystalline Der p 2 atomic coordinates appear to be promising.

Pyroglyphidae/chemistry/immunology↗

Acute effects of whole-body proton irradiation on the immune system of the mouse

The acute effects of proton whole-body irradiation on the distribution and function of leukocyte populations in the spleen and blood were examined and compared to the effects of photons derived from a (60)Co gamma-ray source. Adult female C57BL/6 mice were exposed to a single dose (3 Gy at 0.4 Gy/min) of protons at spread-out Bragg peak (SOBP), protons at the distal entry (E) region, or gamma rays and killed humanely at six different times thereafter. Specific differences were noted in the results, thereby suggesting that the kinetics of the response may be variable. However, the lack of significant differences in most assays at most times suggests that the RBE for both entry and peak regions of the Bragg curve was essentially 1.0 under the conditions of this study. The greatest immunodepression was observed at 4 days postexposure. Flow cytometry and mitogenic stimulation analyses of the spleen and peripheral blood demonstrated that lymphocyte populations differ in radiosensitivity, with B (CD19(+)) cells being most sensitive, T (CD3(+)) cells being moderately sensitive, and natural killer (NK1.1(+)) cells being most resistant. B lymphocytes showed the most rapid recovery. Comparison of the T-lymphocyte subsets showed that CD4(+) T helper/inducer cells were more radiosensitive than the CD8(+) T cytotoxic/suppressor cells. These findings should have an impact on future studies designed to maximize protection of normal tissue during and after proton-radiation exposure.

NASA Discipline Radiation Health↗

Crystallization and Preliminary X-ray Analysis of Der f 2, a Potent Allergen Derived from the House Dust Mite

Although a number of allergens have been identified and isolated, the underlying molecular basis for the potent immune response is poorly understood. House dust mites (Dermatophugoides sp.) are particularly ubiquitous contributors to atopy in developed countries. The rhinitis, dermatitis, and asthma associated with allergic reactions to these arthropods are often caused by relatively small (125-129 amino acids) mite proteins of unclear biological function. Der f 2, a major allergen from the mite Dermatophagoides farinae, has been recombinantly expressed and characterized. The Der f 2 protein has been crystallized in our laboratory and a native data set collected at a synchrotron source. The crystals belong to the orthorhombic space group I422 with unit cell parameters of a = 95.2 Angstroms, b = 95.2 Angstroms, and c = 103.3 Angstroms. An essentially complete (97.2%) data set has been collected to 2.4 Angstroms. Attempts to solve the crystal structure of Der f 2 by molecular replacement using the available NMR coordinates for either Der f 2 or Der p 2 (the homologous protein from D. pterovssinus) failed to reveal a creditable solution.

Roeber, Dana↗

Microgravity and Cellular Consequences in Lymphocyte Function

Mammalian cells adapt to the environment of low gravity and express a series of responses, some possibly from direct effects on cells and others based on environmental conditions created by microgravity. Human lymphocytes in microgravity culture are functionally diminished in activation and locomotion. Both processes are integral to optimal immune response to fight pathogens. The NASA Rotating-wall vessel (RWV) is a well-accepted analog for microgravity culture on the ground. Gene array experiments and immunoblotting identified upstream events in human lymphocytes adapting to microgravity analog culture. Microgravity induces selective changes, many of which are cell membrane related. Results showed that upstream of PKC in the T cell activation cascade, PLC-gamma and LAT are significantly diminished. ZAP 70 which controls LAT activation is also down regulated in modeled microgravity. Thus events governing cell shape might warrant attention in microgravity conditions. The goal of this study is to delineate response suites that are consequential, direct or indirect effects of the microgravity environment and which of these are essential to lymphocytes

Pellis, Neal R.↗