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NASA NTRS2009

Enceladus: An Active Cryovolcanic Satellite

Enceladus is one of the most remarkable satellites in the solar system, as revealed by Cassini's detection of active plumes erupting from warm fractures near its south pole. This discovery makes Enceladus the only icy satellite known to exhibit ongoing internally driven geological activity. The activity is presumably powered by tidal heating maintained by Enceladus 2:1 mean-motion resonance with Dione, but many questions remain. For instance, it appears difficult or impossible to maintain the currently observed radiated power (probably at least 6 GW) in steady state. It is also not clear how Enceladus first entered its current self-maintaining warm and dissipative state initial heating from non-tidal sources is probably required. There are also many unanswered questions about Enceladus interior. The silicate fraction inferred from its density of 1.68 g per cubic centimeter is probably differentiated into a core, though we have only indirect evidence for differentiation. Above the core there is probably a global or regional water layer, inferred from several models of tidal heating, and an ice shell thick enough to support the ~1 kilometer amplitude topography seen on Enceladus. It is possible that dissipation is largely localized beneath the south polar region. Enceladus surface geology, ranging from moderately cratered terrain to the virtually crater-free active south polar region, is highly diverse, tectonically complex, and remarkably symmetrical about the rotation axis and the direction to Saturn. South polar activity is concentrated along the four tiger stripe fractures, which radiate heat at temperatures up to at least 167 K and are the source of multiple plumes ejecting ~200 kilograms per second of H2O vapor along with significant N2 (or C2H4), CO2, CH4, NH3, and higher-mass hydrocarbons. The escaping gas maintains Saturn's neutral gas torus, and the plumes also eject a large number of micron-sized H2O ice grains that populate Saturn's E-ring. The mechanism that powers the plumes is not well understood, and whether liquid water is involved is a subject of active debate (but likely nonetheless). Enceladus provides a promising potential habitat for life in the outer solar system, and the active plumes allow the unique opportunity for direct sampling of that zone. Enceladus is thus a prime target for Cassini's continued exploration of the Saturn system, and will be a tempting target for future missions.

Spencer, J. R.↗
NASA NTRSDate not supplied

Extraterrestrial Molecular Indicators of Life Investigation (EMILI)

Future missions to Enceladus, Europa, Mars, and beyond may seek the molecular signs of extraterrestrial life through chemical analysis of acquired samples. Particularly on ocean worlds such as Enceladus and Europa, samples may contain trace ocean-borne molecular biosignatures of extant life that may or may not share similarities to those of terrestrial life. In situ analyses must be prepared to detect and characterize a wide range of possible molecular species, structures, and patterns, typically with exquisite sensitivity and within a complex, poorly-characterized planetary environment. The Extraterrestrial Molecular Indicators of Life Investigation (EMILI) is designed to meet or exceed the requirements of such missions for organic molecular analysis through a powerful combination of dual chemical separation and both optical and mass spectrometry detection techniques, realized in an integrated, compact instrument package fully compatible with anticipated flight resources and conditions. The full EMILI instrument combines two sample analysis subsystems to provide wide-ranging and complementary detection of organic compounds and inorganic salts. The Gas Analysis Processing System (GAPS) uses a chemical derivatization protocol with gas chromatography (GC) separation prior to detection in an ion trap mass spectrometer (ITMS) to enable full characterization of lower-polarity, volatile and semi-volatile molecules such as fatty acids and hydrocarbons. The Organic Capillary Electrophoresis ANalysis System (OCEANS) uses a liquid-based extraction protocol with CE separation to enable precise analysis of more water-soluble/polar compounds. OCEANS features a laser-induced fluorescence detection mode to perform ultra-sensitive quantitative analysis of chiral amino acids. In EMILI, OCEANS is additionally coupled to the same ITMS through a novel electrospray ionization interface. The common ITMS allows EMILI to identify and cross-correlate molecular species and patterns, detected through either or both protocols, of molecular weights to over 1000 u, potentially even revealing complex biosignatures such as alien oligopeptides and informational polymers.

Europa↗
NASA NTRSDate not supplied

CONTAMINATION CONTROL FOR ULTRA-SENSITIVE LIFE DETECTION SCIENCE MISSIONS

"This study report summarizes technological developments in science-required contamination control for cost-capped, life-detection missions in the Solar System. Technology advances focus on developing implementable strategies to restrict contamination of the spacecraft and instruments by the launch vehicle hardware. Secondary bake out operations during cruise further reduce molecular contaminants down to femtomolar levels in the sample path. The study validated a full-spacecraft, deployable barrier design as an effective strategy to isolate the spacecraft from the pre-launch processing and launch environment (e.g., fairing acoustic materials). It accommodates late mounting of RTGs and other required prelaunch activities. A new, high-fidelity physics, contamination-transport model for particles (including cells and their parts) and science-relevant molecules (e.g., possible biomolecules) was developed to validate the barrier concept. Importantly, this model takes into account the physics of extremely clean surfaces achieved by the best traditional contamination engineering. The ability to deal with very low levels of particulate and molecular contamination makes this model unique among standard contamination modeling approaches. Modeling also demonstrated that a high-temperature bake out during interplanetary cruise of the sample collector that comprises the largest surface area in the sample path for an Enceladus orbiter reference mission, was extremely effective. This study included the evaluation of other details necessary for an effective contamination control strategy for life detection missions including feasibility of achieving parts per trillion level cleanliness for semi-volatile hydrocarbons in nitrogen purge gas, identification of launch vehicle services required for the baseline contamination control, and defining additional contamination control measures to be taken during launch operations. Key results of the study are: • New spacecraft barrier design that accommodates RTGs is readily cleanable and repairable. Deployment of a 1/3 scale model was successfully demonstrated. • The barrier reduces particle contamination (likely biological) from fairing to spacecraft by 1e-2 to 1e-3. • On-cruise bake-out of critical surfaces significantly reduced molecular contamination (by as much as 1e-12). Subsequently, the probability of a surface contamination particle being transported to an instrument by an ice particle is less than 5.1e-5 (for microbes specifically, 4.39e-10). The report concludes that a full-spacecraft barrier designed specifically for life-detection missions that require stringent control of particulate and molecular contaminates is an effective means of mitigating risks of false positive and negative results for life signature investigations. Further, secondary cleaning steps for critical sections of the sample path can be highly effective at reducing molecular contaminants to low femtomolar levels."

Contamination↗
NASA NTRSDate not supplied

Contamination Control for Ultra-Sensitive Life-Detection Missions

"This study report summarizes technological developments in science-required contamination control for cost-capped, life-detection missions in the Solar System. Technology advances focus on developing implementable strategies to restrict contamination of the spacecraft and instruments by the launch vehicle hardware. Secondary bake out operations during cruise further reduce molecular contaminants down to femtomolar levels in the sample path. The study validated a full-spacecraft, deployable barrier design as an effective strategy to isolate the spacecraft from the pre-launch processing and launch environment (e.g., fairing acoustic materials). It accommodates late mounting of RTGs and other required prelaunch activities. A new, high-fidelity physics, contamination-transport model for particles (including cells and their parts) and science-relevant molecules (e.g., possible biomolecules) was developed to validate the barrier concept. Importantly, this model takes into account the physics of extremely clean surfaces achieved by the best traditional contamination engineering. The ability to deal with very low levels of particulate and molecular contamination makes this model unique among standard contamination modeling approaches. Modeling also demonstrated that a high-temperature bake out during interplanetary cruise of the sample collector that comprises the largest surface area in the sample path for an Enceladus orbiter reference mission, was extremely effective. This study included the evaluation of other details necessary for an effective contamination control strategy for life detection missions including feasibility of achieving parts per trillion level cleanliness for semi-volatile hydrocarbons in nitrogen purge gas, identification of launch vehicle services required for the baseline contamination control, and defining additional contamination control measures to be taken during launch operations. Key results of the study are: • New spacecraft barrier design that accommodates RTGs is readily cleanable and repairable. Deployment of a 1/3 scale model was successfully demonstrated. • The barrier reduces particle contamination (likely biological) from fairing to spacecraft by 1e-2 to 1e-3. • On-cruise bake-out of critical surfaces significantly reduced molecular contamination (by as much as 1e-12). Subsequently, the probability of a surface contamination particle being transported to an instrument by an ice particle is less than 5.1e-5 (for microbes specifically, 4.39e-10). The report concludes that a full-spacecraft barrier designed specifically for life-detection missions that require stringent control of particulate and molecular contaminates is an effective means of mitigating risks of false positive and negative results for life signature investigations. Further, secondary cleaning steps for critical sections of the sample path can be highly effective at reducing molecular contaminants to low femtomolar levels."

contamination, life detection, barrier, modeling↗
NASA NTRS2021

The Dual-Rasp Sampling System Design with Closed Pneumatic Sample Transfer

The novel Dual-Rasp sampling system has been developed for landed missions to low gravity planetary bodies and is particularly well suited for the unique environment of Saturn’s moon Enceladus. The Dual-Rasp sampling system has two counter-rotating rasp cutters that remove material from the surface and direct it into a guide. The cuttings follow the guide into a sample collection cup. When the sampling collection completes, the tool reconfigures by rotating the guide to create a closed circuit for pneumatic sample transfer from the collection cup to science instruments on the lander. A valve opens a gas tank and the gas flows from that tank into the sample collection cup and down rigid tubing to the science instruments chamber on the lander. A two degree of freedom arm with base actuators is used to deploy the sampler and control its sampling location.

Heness, Andrew B.↗
NASA NTRS2008

An Organic Decontamination Method for Sampling Devices used in Life-detection Studies

Organic decontamination of sampling and storage devices are crucial steps for life-detection, habitability, and ecological investigations of extremophiles living in the most inhospitable niches of Earth, Mars and elsewhere. However, one of the main stumbling blocks for Mars-analogue life-detection studies in terrestrial remote field-sites is the capability to clean instruments and sampling devices to organic levels consistent with null values. Here we present a new seven-step, multi-reagent cleaning and decontamination protocol that was adapted and tested on a glacial ice-coring device and on a rover-guided scoop used for sediment sampling both deployed multiple times during two field seasons of the Arctic Mars Analog Svalbard Expedition AMASE). The effectiveness of the protocols for both devices was tested by (1)in situ metabolic measurements via APT, (2)in situ lipopolysacchride (LPS) quantifications via low-level endotoxin assays, and(3) laboratory-based molecular detection via gas chromatography-mass spectrometry. Our results show that the combination and step-wise application of disinfectants with oxidative and solvation properties for sterilization are effective at removing cellular remnants and other organic traces to levels necessary for molecular organic- and life-detection studies. The validation of this seven-step protocol - specifically for ice sampling - allows us to proceed with confidence in kmskia4 analogue investigations of icy environments. However, results from a rover scoop test showed that this protocol is also suitable for null-level decontamination of sample acquisition devices. Thus, this protocol may be applicable to a variety of sampling devices and analytical instrumentation used for future astrobiology missions to Enceladus, and Europa, as well as for sample-return missions.

Eigenbrode, Jennifer↗
NASA NTRS2008

Proceedings of the 39th Lunar and Planetary Science Conference

Sessions with oral presentations include: A SPECIAL SESSION: MESSENGER at Mercury, Mars: Pingos, Polygons, and Other Puzzles, Solar Wind and Genesis: Measurements and Interpretation, Asteroids, Comets, and Small Bodies, Mars: Ice On the Ground and In the Ground, SPECIAL SESSION: Results from Kaguya (SELENE) Mission to the Moon, Outer Planet Satellites: Not Titan, Not Enceladus, SPECIAL SESSION: Lunar Science: Past, Present, and Future, Mars: North Pole, South Pole - Structure and Evolution, Refractory Inclusions, Impact Events: Modeling, Experiments, and Observations, Mars Sedimentary Processes from Victoria Crater to the Columbia Hills, Formation and Alteration of Carbonaceous Chondrites, New Achondrite GRA 06128/GRA 06129 - Origins Unknown, The Science Behind Lunar Missions, Mars Volcanics and Tectonics, From Dust to Planets (Planetary Formation and Planetesimals):When, Where, and Kaboom! Astrobiology: Biosignatures, Impacts, Habitability, Excavating a Comet, Mars Interior Dynamics to Exterior Impacts, Achondrites, Lunar Remote Sensing, Mars Aeolian Processes and Gully Formation Mechanisms, Solar Nebula Shake and Bake: Mixing and Isotopes, Lunar Geophysics, Meteorites from Mars: Shergottite and Nakhlite Invasion, Mars Fluvial Geomorphology, Chondrules and Chondrule Formation, Lunar Samples: Chronology, Geochemistry, and Petrology, Enceladus, Venus: Resurfacing and Topography (with Pancakes!), Overview of the Lunar Reconnaissance Orbiter Mission, Mars Sulfates, Phyllosilicates, and Their Aqueous Sources, Ordinary and Enstatite Chondrites, Impact Calibration and Effects, Comparative Planetology, Analogs: Environments and Materials, Mars: The Orbital View of Sediments and Aqueous Mineralogy, Planetary Differentiation, Titan, Presolar Grains: Still More Isotopes Out of This World, Poster sessions include: Education and Public Outreach Programs, Early Solar System and Planet Formation, Solar Wind and Genesis, Asteroids, Comets, and Small Bodies, Carbonaceous Chondrites, Chondrules and Chondrule Formation, Chondrites, Refractory Inclusions, Organics in Chondrites, Meteorites: Techniques, Experiments, and Physical Properties, MESSENGER and Mercury, Lunar Science Present: Kaguya (SELENE) Results, Lunar Remote Sensing: Basins and Mapping of Geology and Geochemistry, Lunar Science: Dust and Ice, Lunar Science: Missions and Planning, Mars: Layered, Icy, and Polygonal, Mars Stratigraphy and Sedimentology, Mars (Peri)Glacial, Mars Polar (and Vast), Mars, You are Here: Landing Sites and Imagery, Mars Volcanics and Magmas, Mars Atmosphere, Impact Events: Modeling, Experiments, and Observation, Ice is Nice: Mostly Outer Planet Satellites, Galilean Satellites, The Big Giant Planets, Astrobiology, In Situ Instrumentation, Rocket Scientist's Toolbox: Mission Science and Operations, Spacecraft Missions, Presolar Grains, Micrometeorites, Condensation-Evaporation: Stardust Ties, Comet Dust, Comparative Planetology, Planetary Differentiation, Lunar Meteorites, Nonchondritic Meteorites, Martian Meteorites, Apollo Samples and Lunar Interior, Lunar Geophysics, Lunar Science: Geophysics, Surface Science, and Extralunar Components, Mars, Remotely, Mars Orbital Data - Methods and Interpretation, Mars Tectonics and Dynamics, Mars Craters: Tiny to Humongous, Mars Sedimentary Mineralogy, Martian Gullies and Slope Streaks, Mars Fluvial Geomorphology, Mars Aeolian Processes, Mars Data and Mission,s Venus Mapping, Modeling, and Data Analysis, Titan, Icy Dwarf Satellites, Rocket Scientist's Toolbox: In Situ Analysis, Remote Sensing Approaches, Advances, and Applications, Analogs: Sulfates - Earth and Lab to Mars, Analogs: Remote Sensing and Spectroscopy, Analogs: Methods and Instruments, Analogs: Weird Places!. Print Only Early Solar System, Solar Wind, IDPs, Presolar/Solar Grains, Stardust, Comets, Asteroids, and Phobos, Venus, Mercury, Moon, Meteorites, Mars, Astrobiology, Impacts, Outer Planets, Satellites, and Rings, Support for Mission Operations, Analog Education and Public Outreach.

Source record↗
NASA NTRS2016

Sylph - A SmallSat Probe Concept Engineered to Answer Europa's Big Question

Europa, the Galilean satellite second from Jupiter, contains a vast, subsurface ocean of liquid water. Recent observations indicate possible plume activity. If such a plume expels ocean water into space as at Enceladus, a spacecraft could directly sample the ocean by analyzing the plume’s water vapor, ice, and grains. Due to Europa’s strong gravity, such sampling would have to be done within 5 km of the surface to sample ice grains larger than 5 µm, expected to be frozen ocean spray and thus to contain non-volatile species critical to a biosignature-detection mission. By contrast, the planned Europa Multiple Flyby Mission’s closest planned flyby altitude is 25 km. Sylph is a concept for a SmallSat free-flyer probe that, deployed from the planned Europa Mission, would directly sample the large grains by executing a single ~2-km altitude plume pass. The 40-kg probe would be deployed by the Europa mission just before it executes a plume fly-through. Within the probe’s 16-hour lifespan, it would autonomously navigate to perform a parallel, simultaneous pass at the lower altitude. The Sylph flight system design concept combines SmallSat technologies with robust traditional components and advanced manufacturing technologies. Its payload would comprise Mini-SUDA (SUrface Dust Mass Analyzer), a dual-channel, miniature impact ionization mass spectrometer. Sylph represents a novel type of SmallSat concept: purpose-built configuration, optimized for the harsh environment at Europa and for planetary-protection requirements, and hybridized from both mainstream and SmallSat components.

Imken, Travis↗
NASA NTRS2024

Future of the Search for Life: Workshop Report

The 2-week, virtual Future of the Search for Life science and engineering workshop brought together more than 100 scientists, engineers, and technologists in March and April 2022 to provide their expert opinion on the interconnections between life-detection science and technology. Participants identified the advances in measurement and sampling technologies they believed to be necessary to perform in situ searches for life elsewhere in our Solar System, 20 years or more in the future. Among suggested measurements for these searches, those pertaining to three potential indicators of life termed “dynamic disequilibrium,” “catalysis,” and “informational polymers” were identified as particularly promising avenues for further exploration. For these three indicators, small breakout groups of participants identified measurement needs and knowledge gaps, along with corresponding constraints on sample handling (acquisition and processing) approaches for a variety of environments on Enceladus, Europa, Mars, and Titan. Despite the diversity of these environments, sample processing approaches all tend to be more complex than those that have been implemented on missions or envisioned for mission concepts to date. The approaches considered by workshop breakout groups progress from nondestructive to destructive measurement techniques, and most involve the need for fluid (especially liquid) sample processing. Sample processing needs were identified as technology gaps. These gaps include technology and associated sampling strategies that allow the preservation of the thermal, mechanical, and chemical integrity of the samples upon acquisition; and to optimize the sample information obtained by operating suites of instruments on common samples. Crucially, the interplay between science-driven life-detection strategies and their technological implementation highlights the need for an unprecedented level of payload integration and extensive collaboration between scientists and engineers, starting from concept formulation through mission deployment of life-detection instruments and sample processing systems.

Marc Neveu↗
NASA NTRS2017

Mobile/Modular BSL-4 Facilities for Meeting Restricted Earth Return Containment Requirements

NASA robotic sample return missions designated Category V Restricted Earth Return by the NASA Planetary Protection Office require sample containment and biohazard testing in a receiving laboratory as directed by NASA Procedural Requirement (NPR) 8020.12D - ensuring the preservation and protection of Earth and the sample. Currently, NPR 8020.12D classifies Restricted Earth Return for robotic sample return missions from Mars, Europa, and Enceladus with the caveat that future proposed mission locations could be added or restrictions lifted on a case by case basis as scientific knowledge and understanding of biohazards progresses. Since the 1960s, sample containment from an unknown extraterrestrial biohazard have been related to the highest containment standards and protocols known to modern science. Today, Biosafety Level (BSL) 4 standards and protocols are used to study the most dangerous high-risk diseases and unknown biological agents on Earth. Over 30 BSL-4 facilities have been constructed worldwide with 12 residing in the United States; of theses, 8 are operational. In the last two decades, these brick and mortar facilities have cost in the hundreds of millions of dollars dependent on the facility requirements and size. Previous mission concept studies for constructing a NASA sample receiving facility with an integrated BSL-4 quarantine and biohazard testing facility have also been estimated in the hundreds of millions of dollars. As an alternative option, we have recently conducted an initial trade study for constructing a mobile and/or modular sample containment laboratory that would meet all BSL-4 and planetary protection standards and protocols at a faction of the cost. Mobile and modular BSL-2 and 3 facilities have been successfully constructed and deployed world-wide for government testing of pathogens and pharmaceutical production. Our study showed that a modular BSL-4 construction could result in approximately 90% cost reduction when compared to traditional construction methods without compromising the preservation of the sample or Earth.

Calaway, M. J.↗
NASA NTRS2014

Science Goals and Mission Concept for the Future Exploration of Titan and Enceladus

Saturn׳s moons, Titan and Enceladus, are two of the Solar System׳s most enigmatic bodies and are prime targets for future space exploration. Titan provides an analogue for many processes relevant to the Earth, more generally to outer Solar System bodies, and a growing host of newly discovered icy exoplanets. Processes represented include atmospheric dynamics, complex organic chemistry, meteorological cycles (with methane as a working fluid), astrobiology, surface liquids and lakes, geology, fluvial and aeolian erosion, and interactions with an external plasma environment. In addition, exploring Enceladus over multiple targeted flybys will give us a unique opportunity to further study the most active icy moon in our Solar System as revealed by Cassini and to analyse in situ its active plume with highly capable instrumentation addressing its complex chemistry and dynamics. Enceladus׳ plume likely represents the most accessible samples from an extra-terrestrial liquid water environment in the Solar system, which has far reaching implications for many areas of planetary and biological science. Titan with its massive atmosphere and Enceladus with its active plume are prime planetary objects in the Outer Solar System to perform in situ investigations. In the present paper, we describe the science goals and key measurements to be performed by a future exploration mission involving a Saturn-Titan orbiter and a Titan balloon, which was proposed to ESA in response to the call for definition of the science themes of the next Large-class mission in 2013. The mission scenario is built around three complementary science goals: (A) Titan as an Earth-like system; (B) Enceladus as an active cryovolcanic moon; and (C) Chemistry of Titan and Enceladus - clues for the origin of life. The proposed measurements would provide a step change in our understanding of planetary processes and evolution, with many orders of magnitude improvement in temporal, spatial, and chemical resolution over that which is possible with Cassini-Huygens. This mission concept builds upon the successes of Cassini-Huygens and takes advantage of previous mission heritage in both remote sensing and in situ measurement technologies.

future missions↗
NASA NTRS2016

Trajectories for Flyby Sample Return at Saturn's Moons

Ballistic trajectories are computed which would enable a sample return mission to Titan or Enceladus without capturing, descending, or landing. The low-cost mission concept utilizes a free return trajectory that also involves a close flyby of the moon. This work extends the concept, and related trajectory analysis methodology, previously applied to a Europa mission. Specifically, a broad search algorithm is employed to systematically locate potentially feasible itineraries over an entire Saturn period. High-quality approximate solutions are then optimized to be continuous using high-fidelity dynamics. Techniques and software from the Europa analysis, were readily adapted and able to find numerous mission enabling trajectories. A direct mission to Titan is possible with flight time under 16 years and Earth-relative speeds below 11.0 km/sec. The VEEGA option is shown to substantially reduce launch C3, but flight times exceed 21 years. Unfortunately, an Enceladus mission requires a flight time of 25 years or more, and incurs fairly high relative speeds. Nevertheless, an optimized reference mission is computed.

Jones, Drew Ryan↗
NASA NTRS2016

A Fly-Through Mission Strategy Targeting Peptide as a Signature of Chemical Evolution and Possible Life in Enceladus Plumes

In situ detection of organic molecules in the extraterrestrial environment provides a key step towards better understanding the variety and the distribution of building blocks of life and it may ultimately lead to finding extraterrestrial life within the Solar System. Here we present combined results of two separate experiments that enable us to realize such in situ life signature detection from the deep habitats of the "Ocean World": a hydrothermal reactor experiment simulating complex organic synthesis and a simulated fly-through capture experiment of organic-bearing microparticles using silica aerogels, followed by subsequent analysis. Both experiments employ peptide as a plausible organics existing in Encleadus plume particles produced in its subsurface ocean. Recent laboratory hydrothermal experiments and a theoretical model on silica saturation indicated an on going hydrothermal reactions in subsurface Enceladus ocean. Given the porous chondritic origin of the core, it is likely that organic compounds originated by radiation chemistry such as amino acid precursors could have been provided, leached, and altered through widespread water-rock interactions. By using the same laboratory experimental setup from the latest water-rock interaction study, we performed amino acid polymerization experiments for 144 days and monitored the organic complexity changing over time. So far over 3,000 peaks up to the size of greater than 600 MW were observed through the analysis of capillary electrophoresis time-of-flight mass spectrometry (CE-TOF-MS) with an indication of amino acid derivatives and short peptides. Generally abiotic polymerization of enantiomeric amino acids results in forming stereoisomeric peptides with identical molecular weight and formula as opposed to homochiral biopolymers. Assuming Enceladus plume particles may contain a mixture of stereoisomeric peptides, we were able to distinguish 16 of the 17 stereoisomeric tripeptides as a test sample using capillary electrophoresis (CE) under optimized conditions. We further conducted Enceladus plume fly-through capture experiment by accelerating peptides soaked in rock particles up to a speed of 5.7 km/s and capturing with originally developed hydrophobic silica aerogels. Direct peptide extraction with acetonitrile-water followed by CE analysis led to detection of only but two stereoisomeric acidic peptide peaks, presenting the first run-through hypervelocuty impact sample analysis targeting peptides as key molecule to to understand the ongoing astrobiology on Enceladus.

organic molecules↗
NASA NTRSDate not supplied

Squeezing Every Last 'Bit' of Information from Enceladus Mass Spectrometry

Potential opportunities to return to Enceladus in Discovery and Flagship class missions inspire development of next-generation instruments and creative approaches to sample collection, sample analysis, and data analysis and transmission strategies. Mass spectrometers (MS) are ideally suited to future Enceladus missions due to their analytical power in identifying a range of molecular and ionic compositions – including complex organics – and potentially astrobiologically-important features such as isotope ratios, chirality, and enantiomeric excess. However, long communication delays from Enceladus and limited bandwidth limits the data transmission from these higher-data-volume instruments, likely delaying mission-related response to new data. We explore the utility of data science and machine learning (ML) on isotope ratio (IR)MS data collected from laboratory analogs of Enceladus to: 1) process data quickly for rapid ground-based analyses, 2) understand if compositional and biosignature information could be extracted from IRMS data, and 3) evaluate whether onboard ML techniques could improve sample analysis, cadence, and transmission prioritization. Laboratory analogs analyzed isotopes of volatile CO2 that interacted with seawaters of varying composition, and include both abiotic and biotic (microbially-influenced) experiments. Enceladus’s alkaline oceans promote speciation of carbon into multiple forms (e.g., H2CO3 / CO2, HCO3-, and CO32-), each of which could be isotopically fractionated by abiotic or biotic reactions. Large (>2‰) changes in carbon isotopes (δ13C) are observed from some biotic experiments inoculated with complex microbial ecosystems relative to the abiotic seawaters. ML training and classification suggests that microbial samples can be distinguished from abiotic samples, yet that a broad range of microbial experiments are necessary to train ML models to cover a range of complexities including disequilibria, and isotopic and compositional fractionation.

geochemistry↗
NASA NTRS2017

Sample Processor for Life on Icy Worlds (SPLIce): Design and Test Results

We report the design, development, and testing of the Sample Processor for Life on Icy Worlds (SPLIce) system, a microfluidic sample processor to enable autonomous detection of signatures of life and measurements of habitability parameters in Ocean Worlds. This monolithic fluid processing-and-handling system (Figure 1; mass 0.5 kg) retrieves a 50-L-volume sample and prepares it to supply a suite of detection instruments, each with unique preparation needs. SPLIce has potential applications in orbiter missions that sample ocean plumes, such as found in Saturns icy moon Enceladus, or landed missions on the surface of icy satellites, such as Jupiters moon Europa. Answering the question Are we alone in the universe? is captivating and exceptionally challenging. Even general criteria that define life very broadly include a significant role for water [1,2]. Searches for extinct or extant life therefore prioritize locations of abundant water whether in ancient (Mars), or present (Europa and Enceladus) times. Only two previous planetary missions had onboard fluid processing: the Viking Biology Experiments [3] and Phoenixs Wet Chemistry Laboratory (WCL) [4]. SPLIce differs crucially from those systems, including its capability to process and distribute L-volume samples and the integration autonomous control of a wide range of fluidic functions, including: 1) retrieval of fluid samples from an evacuated sample chamber; 2) onboard multi-year storage of dehydrated reagents; 3) integrated pressure, pH, and conductivity measurement; 4) filtration and retention of insoluble particles for microscopy; 5) dilution or vacuum-driven concentration of samples to accommodate instrument working ranges; 6) removal of gas bubbles from sample aliquots; 7) unidirectional flow (check valves); 8) active flow-path selection (solenoid-actuated valves); 9) metered pumping in 100 nL volume increments. The SPLIce manifold, made of three thermally fused layers of precision-machined cyclo-olefin polymer, supports all fluidic components (Figure 1) and integrated microchannels (125 x 250 m). Fluid is pumped by a stepper-motor-driven pump (Lee Co.). The functionality of the integrated MEMS pressure sensor (Honeywell) and passive check valves (Figure 2) were tested in conjunction with our newly designed integral bubble traps (Figure 3) and hydrophobic membrane-based concentrator (Figure 4). The concentrator (initially tested as a standalone component) demonstrated 5-fold vacuum-evaporative concentration. Polyethylene fused bead beds (PEFBBs; 50 porosity) store drylyophilized buffers, calibrants, and fluorescent dyes, and also promote mixing of sample with calibrant, dye, or H2O. Software-controlled automated tests demonstrated successful 1) fluid delivery to each component 2) valve and pump synchronization 3) sample aliquot delivery to instrument interface ports, and 4) rehydration of vacuum-dried fluorescent dye. In Figure 5, fluorescein on PEFBBs was rehydrated for 15 min using a pump-delivered water aliquot; it is displaced as H2O enters the bottom of the channel and pushes the dye into a check valve. Ultimately, SPLIce will fluorescently label amino acids in the sample for microchip-based electrophoretic (MCE) chiral separation and detection to seek and quantify key organic bio-signatures [5]; it will also deliver sample to a microfluidic version of WCL (mWCL) to measure soluble ions and redox-active species.

Life detection↗
NASA NTRS2017

Raman Life Detection Instrument Development for Icy Worlds

The objective of this project is to develop a compact, high sensitivity Raman sensor for detection of life signatures in a flow cell configuration to enable bio-exploration and life detection during future mission to our Solar Systems Icy Worlds. The specific project objectives are the following: 1) Develop a Raman spectroscopy liquid analysis sensor for biosignatures; 2) Demonstrate applicability towards a future Enceladus or other Icy Worlds missions; 3) Establish key parameters for integration with the ARC Sample Processor for Life on Icy Worlds (SPLIce); 4) Position ARC for a successful response to upcoming Enceladus or other Icy World mission instrument opportunities.

astrobiolog↗
DOE OSTI2021

Stars in the local galactic thick disc and halo in Gaia EDR3: a catalogue of half a million local main-sequence stars with photometric metallicities

We present a catalogue of 551 214 main-sequence stars in the local (d < 2 kpc) Galactic thick disc and halo, based on a search of stars with large proper motions ($\mu _{\rm tot} \gt 40.0~\rm mas\, yr^{-1}$) in the Gaia Early Data Release 3. We derive photometric metallicity calibrated from the colour–luminosity–metallicity distribution of 20 047 stars with spectroscopic metallicities, collected from various spectroscopic surveys, including SDSS SEGUE/APOGEE, GALAH DR3, and LAMOST DR6. We combine these results to construct an empirical colour–magnitude–metallicity grid, which can be used to estimate photometric metallicities for low-mass metal-poor stars of K and M subtypes from their absolute G magnitude and colour values. We find that low-mass, high-velocity stars in our catalogue share similar kinematics as reported in recent studies of more luminous Galactic halo stars. The pseudo-kinematic analysis of our sample recovers the main local halo structures, including the Gaia–Enceladus Stream and the Helmi stream; aside from these, the local halo stars appear to show a remarkably smooth distribution in velocity space. Since the future Gaia data release will provide radial velocity measurements for only a small number of our sample, our catalogue provides targets of high interest for the future spectroscopic observation programs.

79 ASTRONOMY AND ASTROPHYSICS↗
NASA NTRS2020

Encealdus Orbilander: A Flagship Mission Concept for Astrobiology

"Whether life exists beyond Earth remains a fundamental question driving our exploration of the Solar System. At Saturn’s moon Enceladus, plumes of oceanic material vented into space allow the investigation of the astrobiological potential of an ocean world, hinted at by Cassini, without the necessity of drilling through kilometers of ice crust. The Enceladus Orbilander is a flagship ($2.56B in fiscal year 2025 dollars) mission concept created for the 2023–2033 Planetary Science Decadal Survey. Orbilander takes full advantage of the opportunity provided by Enceladus’ plumes to search for signs of life. A single spacecraft both orbits and lands, capturing samples from four distinct reservoirs offered by the plumes. These samples, both particulate and vapor, are then analyzed by the Life Detection Suite (LDS), a set of five instruments conducting complementary and orthogonal biosignature-seeking measurements. To provide the context that specifically enhances interpretation of LDS measurements, geochemical and geophysical investigations are conducted both in orbit and on the surface. These reveal the physio-chemical state of the ocean and core as well as the processes involved in ejection of plume material and how these affect the ocean material analyzed by the LDS. The Orbilander can be delivered to the Saturn system via several launch vehicle and trajectory options, including a direct trajectory (7-year cruise), a ∆V-EGA trajectory (9-year cruise) and several options using an inner cruise with Venus and Earth flybys (10-year cruise). Upon Saturn Orbit Insertion, a 4-year moon tour pumps down the Orbilander’s orbit to intercept Enceladus. The most optimal arrival times balance the Jupiter flyby opportunities of the late 2030s and solar illumination at the Enceladus high southern latitudes where plume material is most abundant. This mission concept therefore targets project start in 2030. Upon Enceladus Orbit Insertion, the Orbilander begins a 1.5-year-long campaign of landing site reconnaissance, remote sensing science, and collecting sufficient plume sample to run all but one of the LDS measurements. After successful landing, the Orbilander spends 2 years on the surface conducting multiple LDS measurements with all five instruments on actively and passively collected plume material, as well as seismic investigations. The schedule laid out here is well-defined, but the mission also has operational and resource flexibility should additional reconnaissance be needed. As part of the design study, mission and development risks were identified and mitigation strategies proposed. Technologies key to achieving the life detection science objectives include instrumentation matured under programs like COLDTech and ICEE-2, such as aspects of the sampling system and microfluidic devices, as well as well-known techniques like high-resolution and separation-capable mass spectrometers. Autonomous onboard navigation is planned to maintain a halo orbit around Enceladus to enable passive sampling from orbit as well as reconnaissance measurements for use in site selection and landing. Terrain relative navigation is included to ensure safe landing, given that targeted areas may contain landing hazards. Continued development of radioisotope thermoelectric generator (RTG) technology and long-life batteries is essential for this long duration mission. The Enceladus Orbilander represents an optimal point in the trade space of science value versus cost, taking advantage of the extensive knowledge of Enceladus provided by Cassini, how well Enceladus lends itself to a search for life in material from its ocean, and the flexibility afforded by the innovative design developed by the APL team. By taking full advantage of Enceladus’ plumes both in orbit and on the surface, Orbilander represents a robust search for life with complementary and orthogonal biosignatures as well as contextual geophysical and geochemical measurements, determining not only whether Enceladus is inhabited (at levels up to 500,000× scarcer than in Earth’s oceans) but also why. "

Exobiology↗