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At least 37 records · Page 2

Observation Targeting for the Tehachapi Pass and Mid-Columbia Basin: WindSENSE Phase III Project Summary Report

The overall goal of this multi-phased research project known as WindSENSE is to develop an observation system deployment strategy that would improve wind power generation forecasts. The objective of the deployment strategy is to produce the maximum benefit for 1- to 6-hour ahead forecasts of wind speed at hub-height (~80 m). In Phase III of the project, the focus was on the Mid-Columbia Basin region which encompasses the Bonneville Power Administration (BPA) wind generation area shown in Figure 1 that includes Klondike, Stateline, and Hopkins Ridge wind plants. The typical hub height of a wind turbine is approximately 80-m above ground level (AGL). So it would seem that building meteorological towers in the region upwind of a wind generation facility would provide data necessary to improve the short-term forecasts for the 80-m AGL wind speed. However, this additional meteorological information typically does not significantly improve the accuracy of the 0- to 6-hour ahead wind power forecasts because processes controlling wind variability change from day-to-day and, at times, from hour-to-hour. It is also important to note that some processes causing significant changes in wind power production function principally in the vertical direction. These processes will not be detected by meteorological towers at off-site locations. For these reasons, it is quite challenging to determine the best type of sensors and deployment locations. To address the measurement deployment problem, Ensemble Sensitivity Analysis (ESA) was applied in the Phase I portion of the WindSENSE project. The ESA approach was initially designed to produce spatial fields that depict the sensitivity of a forecast metric to a set of prior state variables selected by the user. The best combination of variables and locations to improve the forecast was determined using the Multiple Observation Optimization Algorithm (MOOA) developed in Phase I. In Zack et al. (2010a), the ESA-MOOA approach was applied and evaluated for the wind plants in the Tehachapi Pass region for a period during the warm season. That research demonstrated that forecast sensitivity derived from the dataset was characterized by well-defined, localized patterns for a number of state variables such as the 80-m wind and the 25-m to 1-km temperature difference prior to the forecast time. The sensitivity patterns produced as part of the Tehachapi Pass study were coherent and consistent with the basic physical processes that drive wind patterns in the Tehachapi area. In Phase II of the WindSENSE project, the ESA-MOOA approach was extended and applied to the wind plants located in the Mid-Columbia Basin wind generation area of Washington-Oregon during the summer and to the Tehachapi Pass region during the winter. The objective of this study was to identify measurement locations and variables that have the greatest positive impact on the accuracy of wind forecasts in the 0- to 6-hour look-ahead periods for the two regions and to establish a higher level of confidence in ESA-MOOA for mesoscale applications. The detailed methodology and results are provided in separate technical reports listed in the publications section below. Ideally, the data assimilation scheme used in the Phase III experiments would have been based upon an ensemble Kalman filter (EnKF) that was similar to the ESA method used to diagnose the Mid-Columbia Basin sensitivity patterns in the previous studies. However, running an EnKF system at high resolution is impractical because of the very high computational cost. Thus, it was decided to use a three-dimensional variational (3DVAR) analysis scheme that is less computationally intensive. The objective of this task is to develop an observation system deployment strategy for the mid Columbia Basin (i.e. the BPA wind generation region) that is designed to produce the maximum benefit for 1- to 6-hour ahead forecasts of hub-height (~80 m) wind speed with a focus on periods of large changes in wind speed. There are two tasks in the current project effort designed to validate the ESA observational system deployment approach in order to move closer to the overall goal: (1) Perform an Observing System Experiment (OSE) using a data denial approach. (2) Conduct a set of Observing System Simulation Experiments (OSSE) for the Mid-Columbia basin region.

17 WIND ENERGY↗

Energy Savings Performance Contract Energy Sales Agreements

An energy savings performance contract energy sales agreement - referred to as an ESPC ESA or ESPC with an ESA - is a project structure that uses the multiyear ESPC authority to implement distributed energy projects on federal buildings or land. The distributed energy project implemented under an ESPC ESA is referred to as an ESA energy conservation measure, or ESA ECM.

ENERGY PLANNING, POLICY, AND ECONOMY↗

Performance Assurance Planning: Continuous Design-Level Performance of Each Energy Conservation Measure in a Utility Energy Service Contract is Fundamental to Achieving Projected Results

An energy savings performance contract energy sales agreement (ESPC ESA) is a project structure, similar to a power purchase agreement, that uses the multiyear ESPC authority to implement renewable energy projects (referred to as ESA energy conservation measures [ECMs]) on federal buildings or land. The ESA ECM is initially privately owned for tax-incentive purposes, and the federal agency purchases the electricity it produces. An ESPC ESA is one of the most cost-effective methods for implementing on-site distributed energy projects at civilian federal agency sites.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Energy Savings Performance Contract Energy Sales Agreements

An energy savings performance contract energy sales agreement (ESPC ESA) is a project structure, similar to a power purchase agreement, that uses the multiyear ESPC authority to implement renewable energy projects (referred to as ESA energy conservation measures [ECMs]) on federal buildings or land. The ESA ECM is initially privately owned for tax-incentive purposes, and the federal agency purchases the electricity it produces. An ESPC ESA is one of the most cost-effective methods for implementing on-site distributed energy projects at civilian federal agency sites.

energy savings performance contract↗

Tandem column isolation of zirconium-89 from cyclotron bombarded yttrium targets using an automated fluidic platform: Anion exchange to hydroxamate resin columns

The development of a tandem column purification method for the production of high-purity 89Zr is presented. The primary column was a macroporous strongly basic anion exchange resin on styrene divinylbenzene co-polymer. The secondary column, with an internal volume of ~27 µL, was packed with hydroxamate resin. A condition of inverted selectivity was developed, whereby the 89Zr eluent from the primary column is synonymous with the load condition on the secondary column. The ability to transfer 89Zr from one column to the next allows two sequential column clean-up methods to be performed prior to the final elution of the 89Zr. This approach assures delivery of high purity 89Zr product and assures a 89Zr product that is eluted in a substantially smaller volume than is possible when using the traditionally-employed single hydroxamate resin column method. The tandem column purification process has been implemented into a prototype automated fluidic system. The system is configured with on-line gamma detection so column effluents can be monitored in near-real time. The automated method was tested using seven cyclotron bombarded Y foil targets. It was found that 95.1±1.3% of the 89Zr present in the foils was recovered in the secondary column elution fraction. Furthermore, elution peak analysis of several 89Zr elution profile radiochromatograms made possible the determination of 89Zr recovery as a function of volume; a 89Zr product volume that contains 90% of the mean secondary column elution peak can be obtained in 0.29±0.06 mL (representing 86±5% of the 89Zr activity in the target). This product volume represents a significant increase in isotope product concentration over the predominant method used in the field. In addition to the reduced 89Zr product elution volume, titrations of the 89Zr product with deferoxamine mesylate salt across two preparatory methods resulted in mean effective specific activity (ESA) values of 279 and 340 TBq·mmole-1 and mean bindable metals concentrations ([MB]) of 13.5 and 16.7 nmole·g-1. These ESA and [MB] values infer that the 89Zr product resulting from this tandem column isolation method has the highest purity reported to date.

immunoPET, zirconium-89, laboratory automation, de↗

Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves

Summary The seeds of many nondomesticated plant species synthesize oils containing high amounts of a single unusual fatty acid, many of which have potential usage in industry. Despite the identification of enzymes for unusual oxidized fatty acid synthesis, the production of these fatty acids in engineered seeds remains low and is often hampered by their inefficient exclusion from phospholipids. Recent studies have established the feasibility of increasing triacylglycerol content in plant leaves, which provides a novel approach for increasing energy density of biomass crops. Here, we determined whether the fatty acid composition of leaf oil could be engineered to accumulate unusual fatty acids. Eleostearic acid ( ESA ) is a conjugated fatty acid produced in seeds of the tung tree ( Vernicia fordii ) and has both industrial and nutritional end‐uses. Arabidopsis thaliana lines with elevated leaf oil were first generated by transforming wild‐type, cgi‐58 or pxa1 mutants (the latter two of which contain mutations disrupting fatty acid breakdown) with the diacylglycerol acyltransferases ( DGAT 1 or DGAT 2 ) and/or oleosin genes from tung. High‐leaf‐oil plant lines were then transformed with tung FADX , which encodes the fatty acid desaturase/conjugase responsible for ESA synthesis. Analysis of lipids in leaves revealed that ESA was efficiently excluded from phospholipids, and co‐expression of tung FADX and DGAT 2 promoted a synergistic increase in leaf oil content and ESA accumulation. Taken together, these results provide a new approach for increasing leaf oil content that is coupled with accumulation of unusual fatty acids. Implications for production of biofuels, bioproducts, and plant–pest interactions are discussed.

59 BASIC BIOLOGICAL SCIENCES↗

Definition and Execution of Regulatory requirements for acceptance of the Whooshh Fish Transport System (Abstract)

The purpose of this project is to assist in the definition, summarization, and execution of the regulatory requirements that validate the Whooshh Fish Transport System (WFTS) as an accepted method of fish passage transport for Endangered Species Act- (ESA) listed species; moving these fish past in-stream barriers, such as hydropower facilities. Validation of this technology, through testing by PNNL, means that regulatory agencies will have documented evidence from which to support an approval decision enabling Whooshh to quickly commercialize their product for use in waterways with ESA listed species and provide the hydropower relicense/development marketplace a viable solution to the fish passage problem that threatens the hydropower industry. Given the definition of “take” in the ESA, without validation, commercialization in this space in the U.S. will not happen because the regulatory agencies will not have the determinative study results they require to authorize the use of this new technology that interact with ESA-listed species.

13 HYDRO ENERGY↗

Identification and Characterization of Lipid Droplet-Associated Protein (LDAP) Isoforms from Tung Tree (Vernicia fordii)

Lipid droplets (LDs) are cytoplasmic organelles responsible primarily for the storage of neutral lipids, such as triacyclglycerols (TAGs). Derived from the endoplasmic reticulum bilayer, LDs are composed of a hydrophobic lipid core encased by a phospholipid monolayer and surface-associated proteins. To date, only a relatively few LD ‘coat’ proteins in plants have been identified and characterized, most of which come from studies of the model plant Arabidopsis thaliana. To expand our knowledge of the plant LD proteome, the LD-associated protein (LDAP) family from the tung tree (Vernicia fordii), whose seeds are rich in a commercially valuable TAG containing the conjugated fatty acid α-eleostearic acid (C18:3Δ9cis,11trans,13trans [α-ESA]), was identified and characterized. Based on the tung tree transcriptome, three LDAP isoforms (VfLDAP1-3) were elucidated and the encoded proteins distinctly clustered into three clades along with their respective isoforms from other angiosperm species. Ectopic expression of the VfLDAPs in Nicotiana benthamiana leaves revealed that they localized specifically to LDs and influenced LD numbers and sizes, as well as increasing TAG content and altering TAG fatty acid composition. Interestingly, in a partially reconstructed TAG-ESA biosynthetic pathway, the co-expression of VfLDAP3 and, to a lesser degree, VfLDAP2, significantly increased the content of α-ESA stored within the LDs. These results suggest that the VfLDAPs can influence the steady-state content and composition of TAG in plant cells and that certain LDAP isoforms may have evolved to more efficiently package TAGs into LDs containing unusual fatty acids, such as α-ESA.

Plant Sciences↗

Chronic preclinical safety evaluation of EPO-018B, a pegylated peptidic erythropoiesis-stimulating agent in monkeys and rats

EPO-018B, a synthetic peptide-based erythropoiesis stimulating agent (ESA), is mainly designed for treatment of anemia caused by chronic renal failure and chemotherapy against cancer. It overcomes the deficiencies of currently approved ESA, including the frequent administration of temperature-sensitive recombinant protein and anti-EPO antibody-mediated pure red cell aplasia (PRCA). This study was designed to evaluate the potential chronic toxicity of EPO-018B. Subcutaneous administration doses were designed as 0, 0.2, 1 and 10 mg/kg for six months for 160 rats (20/gender/group) and 0, 0.3, 3 and 20 mg/kg for nine months for 32 monkeys (4/gender/group) once every three weeks. The vehicles received the same volume of physiological saline injection. All animals survived to the scheduled necropsies after six weeks (for rats) and fourteen weeks (for monkeys) recovery period, except for the two high-dose female rats and two high-dose male monkeys, which were considered related to the increased RBCs, chronic blood hyperviscosity and chronic cardiac injury. EPO-018B is supposed to be subcutaneously injected once every month and the intended human therapeutic dose is 0.025 mg/kg. The study findings at 0.2 mg/kg for rats and 0.3 mg/kg for monkeys were considered to be the study NOAEL (the no observed adverse effect level), which were more than ten times the intended human therapeutic dose. Higher doses caused adverse effects related to the liver toxicity, cardiotoxicity, appearance of neutralizing antibodies of EPO-018B and the decrease of serum glucose and cholesterol. Most treatment-induced effects were reversible or revealed ongoing recovery upon the discontinuation of treatment. The sequelae occurred in rats and monkeys were considered secondary to exaggerated pharmacology and would less likely occur in the intended patient population. As to the differences between human beings and animals, the safety of EPO-018B need to be further confirmed in the future clinical studies. - Highlights: • EPO-018B is a newly developed synthetic peptide-based ESA. • The evaluation contained studies of 6 months in rats and 9 months in monkeys. • The safety studies provided further evidences in future clinical trials. • Most toxic effects were reversible and need more evidences in clinical studies.

60 APPLIED LIFE SCIENCES↗

Recycle of Am-241 obtained from long term stored plutonium for use in radioisotope power systems - 15035

Pu-238 has been used as a power source for spacecraft since the early days of space exploration. It has proven to be an effective source of power where the use of solar generated power is impractical. Its application for use in spacecraft power systems was developed by the USA and Russia who have spent many $ 100s of millions on its development and production. However, the original facilities for the production of Pu-238 are no longer in use and existing worldwide stockpiles have reduced to levels sufficient for only one or two further missions. Production of Pu-238 requires considerable facilities including a nuclear reactor and reprocessing plants that are very expensive to build and operate. The European Space Agency (ESA) has assessed the options for post-launch power generation in future European space missions and has made the decision to pursue the use of Am-241 as an alternative isotope to power future European Radioisotope Power Systems (RPS). What makes Am-241 attractive for the European market is its 100% isotopically pure production from the decay of Pu-241 in separated civil plutonium stockpiles. Historically, Europe has relied on collaborations with the USA or Russia to access these nuclear power sources. During 2009, the European Space Agency (ESA) funded a project to examine the cost and practicality of establishing a European source of material suitable for Radioisotope Power Systems (RPS). The study concluded that a small number of radioisotopes could be used in RPSs. On further review two leading candidates, Am-241 and Pu-238, were selected for further study. Whilst Pu-238 was recognized as the isotope of choice for use in RPSs, the costs of setting up a European based production facility was prohibitively expensive. Although Am-241 has a number of limitations compared to Pu-238, the much reduced production costs make Am-241 an attractive option for European based production of RPSs. Despite its lower power density of ∼ 0.11 Wth/g, Am-241 is viewed as a potential alternative to Pu-238 because of its availability within the nuclear fuel cycle. During the reprocessing of spent nuclear fuel plutonium is separated as a chemically pure PuO{sub 2} product, containing around 3-11% w/w of the Pu-241 isotope, and placed into storage. During storage the Pu-241 isotope beta decays to Am-241 with a half-life of 14.4 years. {sub 94}{sup 241}Pu → (β 14.4 y) {sub 95}{sup 241}Am. This process has resulted in many 100 kgs of americium growing into the civil plutonium stockpile in the UK; material that is of value in generating heat sources and is able to be recycled. The key benefit of Am-241 as a potential European RPS source isotope is the availability of feed material along with the facilities to process it. It is expected that aged civil PuO{sub 2} will contain 25-50 g Am-241 per kg PuO{sub 2} depending on the source. A conceptual flowsheet using facilities for separating americium from plutonium is expected to produce on average 8.4 kg Am/year. ESA are now following their published road-map of studies to develop flight ready RTGs and RHUs. This has included the development and testing of the flowsheet to be used for production of Am-241. The project has focused on underpinning the conceptual flowsheet, using aged plutonium in NNL's PuMA laboratory. This has underpinned the design of a plant to produce a steady supply of Am-241. The scope of the overall project is 'store to store' - the starting point is aged plutonium currently housed in existing Sellafield stores and the end point is the storage of separated plutonium and americium powders on the Sellafield site. The full scale process is planned to be housed within the NNL's Central Laboratory in an existing facility designed for plutonium active operations. (authors)

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Interactions between U.S. biofuels policy and the Endangered Species Act

The Renewable Fuel Standard (RFS) is the primary policy governing the use of biofuels in the United States. Following the 2007 enactment of the RFS, there has been substantial expansion in the use of cropland to produce biofuel feedstocks, including corn and soybeans. Both the conversion of land and the ongoing cultivation of these crops can, depending on management practices, lead to negative environmental outcomes including degraded water quality, increased water use, increased greenhouse gas emissions, and loss or degradation of wildlife habitat. These outcomes have the potential to affect threatened and endangered species protected under the U.S. Endangered Species Act (ESA). The ESA requires federal agencies to consult with the U.S. Fish and Wildlife Service or the National Marine Fisheries Service to ensure that an agency's actions are not likely to jeopardize the existence of imperiled species, nor result in the destruction or adverse modification of their habitat. However, formal consultation has not yet been completed between the U.S. Environmental Protection Agency—the agency charged with implementing the RFS—and the wildlife services, thereby hindering mitigation or remediation of potential impacts. This analysis explores the likely effects of the RFS on land use changes and critical habitat, illustrates example pathways by which threatened and endangered species may be affected, and proposes solutions to help redress this harm. The findings suggest a need for further consultation and assessment of the interactions between the RFS and ESA as well as additional research to support the species-conservation and policymaking processes.

09 BIOMASS FUELS↗