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A Launch Requirements Trade Study for Active Space Radiation Shielding for Long Duration Human Missions

A trade study for an active shielding concept based on magnetic fields in a solenoid configuration versus mass based shielding was developed. Monte Carlo simulations were used to estimate the radiation exposure for two values of the magnetic field strength and the mass of the magnetic shield configuration. For each field strength, results were reported for the magnetic region shielding (end caps ignored) and total region shielding (end caps included but no magnetic field protection) configurations. A value of 15 cSv was chosen to be the maximum exposure for an astronaut. The radiation dose estimate over the total shield region configuration cannot be used at this time without a better understanding of the material and mass present in the end cap regions through a detailed vehicle design. The magnetic shield region configuration, assuming the end cap regions contribute zero exposure, can be launched on a single Space Launch System rocket and up to a two year mission can be supported. The magnetic shield region configuration results in two versus nine launches for a comparable mass based shielding configuration. The active shielding approach is clearly more mass efficient because of the reduced number of launches than the mass based shielding for long duration missions.

Singleterry, Robert C., Jr.

Next Generation Flight Displays Using HTML5

The Human Integrated Vehicles and Environments (HIVE) lab at Johnson Space Center (JSC) is focused on bringing together inter-disciplinary talent to design and integrate innovative human interface technologies for next generation manned spacecraft. As part of this objective, my summer internship project centered on an ongoing investigation in to building flight displays using the HTML5 standard. Specifically, the goals of my project were to build and demo "flight-like" crew and wearable displays as well as create a webserver for live systems being developed by the Advanced Exploration Systems (AES) program. In parallel to my project, a LabVIEW application, called a display server, was created by the HIVE that uses an XTCE (XML (Extensible Markup Language) Telemetry and Command Exchange) parser and CCSDS (Consultative Committee for Space Data System) space packet decoder to translate telemetry items sent by the CFS (Core Flight Software) over User Datagram Protocol (UDP). It was the webserver's job to receive these UDP messages and send them to the displays. To accomplish this functionality, I utilized Node.js and the accompanying Express framework. On the display side, I was responsible for creating the power system (AMPS) displays. I did this by using HTML5, CSS and JavaScript to create web pages that could update and change dynamically based on the data they received from the webserver. At this point, I have not started on the commanding, being able to send back to the CFS, portion of the displays but hope to have this functionality working by the completion of my internship. I also created a way to test the webserver's functionality without the display server by making a JavaScript application that read in a comma-separate values (CSV) file and converted it to XML which was then sent over UDP. One of the major requirements of my project was to build everything using as little preexisting code as possible, which I accomplished by only using a handful of JavaScript libraries. As a side project, I created a model of the HIVE lab and Building 29 using SketchUp. I obtained the floorplans of the building from the JSC Geographic Information Systems (GIS), which were computer-aided design (CAD) files, and imported them into SketchUp. I then took those floorplans and created a 3D model of the building from them. Working in conjunction with the Hybrid Reality lab in Building 32, the SketchUp model was imported into Unreal Engine for use with the HTC Vive. Using the Vive, I was able to interact with the model I created in virtual reality (VR). The purpose of this side project was to be able to visualize potential lab layouts and mockup designs as they are in development in order to finalize design decisions. Pending approval, the model that I created will be used in the Build-As-You-Test: Can Hybrid Reality Improve the SE/HSI Design Process project in the fall. Getting the opportunity to work at NASA has been one of the most memorable experiences of my life. Over the course of my internship, I improved my programming and web development abilities substantially. I will take all the skills and experiences I have had while at NASA back to school with me in the fall and hope to pursue a career in the aerospace industry after graduating in the spring.

Greenwood, Brian

In Situ Air Temperature and Humidity Measurements Over Diverse Land Covers in Greenbelt, Maryland, November 2013-November 2015

As our climate changes through time there is an ever-increasing need to quantify how and where it is changing so that mitigation strategies can be implemented. Urban areas have a disproportionate amount of warming due, in part, to the conductive properties of concrete and asphalt surfaces, surface albedo, heat capacity, lack of water, etc. that make up an urban environment. The NASA Climate Adaptation Science Investigation working group at Goddard Space Flight Center in Greenbelt, MD, conducted a study to collect temperature and humidity data at 15 min intervals from 12 sites at the center. These sites represent the major surface types at the center: asphalt, building roof, grass field, forest, and rain garden. The data show a strong distinction in the thermal properties of these surfaces at the center and the difference between the average values for the center compared to a local meteorological station. The data have been submitted to Oak Ridge National Laboratory Distributed Active Archive Center (ORNL-DAAC) for archival in comma separated value (csv) file format (Carroll et al.,2016) and can be found by following this link: http:daac.ornl.govcgi-bindsviewer.pl?ds_id1319.

Carroll, Mark L.

Data are from Mars, Tools are from Venus

Although during the data production phase, the data producers will usually ensure the products to be easily used by the specific power users the products serve. However, most data products are also posted for general public to use. It is not straightforward for data producers to anticipate what tools that these general end-data users are likely to use. In this talk, we will try to help fill in the gap by going over various tools related to Earth Science and how they work with the existing NASA HDF (Hierarchical Data Format) data products and the reasons why some products cannot be visualized or analyzed by existing tools. One goal is for to give insights for data producers on how to make their data product more interoperable. On the other hand, we also provide some hints for end users on how to make tools work with existing HDF data products. (tool category list: check the comments) HDF-EOS tools: HDFView HDF-EOS Plugin, HEG, h4tonccf, hdf-eos2 dumper, NCL, MATLAB, IDL, etc.net; CDF-Java tools: Panoply, IDV, toosUI, NcML, etc.net; CDF-C tools: ArcGIS Desktop, GrADS, NCL, NCO, etc.; GDAL tools: ArcGIS Desktop, QGIS, Google Earth, etc.; CSV tools: ArcGIS Online, MS Excel, Tableau, etc.

hdf

System Applications Software Development and Testing for the Spaceport Command and Control System

Known as "America's Spaceport," one of Kennedy Space Center's (KSC) primary responsibilities is the successful preparation for and launch of rockets into space. KSC's Engineering Software Branch has been tasked with creating a new command and control system that will provide check-out and launch control for future rockets and spacecraft. While work on the software began several years ago, development is ongoing and the operators who use the software on a daily basis have requested several features to improve their user experience. My internship in the fall of 2018 involved developing the source code and unit tests for two of these requested features: "Display Data with Persistence" (DDP) and "Save Events Button" (SEB). DDP's primary goal is to aid with ergonomics. Currently, users must press-and-hold on the mouse button to display information about points on a data plot. Once DDP is integrated, users will have the ability to double-click on a data plot to display that same information with persistence. Independent from DDP, the SEB provides users the ability to take information about different events that occur in the control system and save that data into a simple Comma Separated File (.csv) file format for easier analysis at a future time.

Niemoeller, Samantha

Space Communications and Navigation Validation: Extracting Data for the Strategic Center for Networking, Integration, and Communications Scheduling Algorithms

Efficiency in communication system architecture performance between Space Communications and Navigation (SCaN) assets and missions is crucial, as space communication is varied, complex, and often not utilized to its full potential. The SCaN Strategic Center for Networking, Integration, and Communications (SCENIC) new scheduling algorithms, which are designed to simulate the allocation of resources between SCaN assets and missions, have the potential to simulate an increase of this efficiency; however, they require real-world data to be validated against. The purpose of this project was to extract said validation data, which details the frequency and duration of utilized contact windows between missions and assets in the Near Earth Network (NEN), Space Network (SN), and Deep Space Network (DSN). Stored as images in daily operations summaries (DOSs), the tabular data existed in a variety of file formats such as.pdf, .docx, and .doc. Since the tables were stored as images, ABBYY® FineReader® (ABBYY Software Ltd.) optical character recognition (OCR) was implemented, which is a proprietary software that reads images from text. The comma separated value (CSV) output was utilized as input to a series of MATLAB® (The MathWorks, Inc.) methods for reformatting, at which point it was ready to be machine-read. Finally, the results were converted to a Microsoft Excel format for human readability. Along with being used for validation purposes, the data will also be used to map equipment degradation as a function of time to analyze the reliability of network assets.

Kontur, Noah P.

A Streamlined Approach to Spatial Mapping of Complex 3D Thermal Boundary Condition Data

Spatial mapping of 1D, 2D, and particularly 3D thermal boundary condition data is essential for accurate thermal simulation of spacecraft and launch vehicles. This information typically is available in simple ASCII file format but can be problematic to use for thermal simulations. Difficulties that can be encountered include the ability to directly use the available file format, transformation of the data into the desired reference coordinate system in 3D space, and accurate interpolation of the data onto the thermal calculation domain. This paper presents a streamlined approach to spatial mapping of complex unsymmetrical 3D cold wall heat flux data using the fields’ capability in Simcenter3D. A recent example of 3D thermal plume simulation at MSFC will be employed to illustrate the use of text, csv, and Excel files, specifying coordinate systems for transformation of data in 3D space, and use of the various interpolation schemes available for spatial mapping. Spatial boundary condition verification is also very important, and the spacecraft thermal analyst needs effective visualization tools to develop confidence in the boundary condition definitions. Visualization of the spacecraft plume raw field data, the calculated field data, and the interpolated field data onto the thermal calculation domain will be discussed, with a particular focus on real-time viewing of the interpolated data for available interpolation schemes. The objective of this paper is to outline the modeling techniques used with the integrated thermal analysis of a terrestrial lunar lander demonstrator. Specifically, the techniques outlined herein focus on spatial mapping of convective and radiative exhaust plume results from a computational fluid dynamics (CFD) analysis. Two cases were investigated, however, the modeling techniques that were utilized translate to both cases seamlessly. Those two cases were as follows: • Lunar lander demonstrator firing on-pad (worst case). • Lunar lander demonstrator hovering at an elevated position above the launch pad. For the purposes of information control, this paper will focus on the modeling techniques only, using altered or dimensionless data where necessary. BACKGROUND The thermal model examined in this paper is of the lunar lander demonstrator known as XL-1T (terrestrial), born from a collaborative effort between Masten Space Systems (MSS) and NASA, under NASA’s Lunar CATALYST (also known as Lunar Cargo Transportation and Landing by Soft Touchdown) initiative. The lander is a reusable vertical takeoff/vertical landing (VTVL) test bed which is controlled by four throttleable main engines utilizing green hypergolic propellants.

Hawkins, Robert F.

NASA's Fire Information for Resource Management System (FIRMS): Near Real-Time Global Fire Monitoring Using Data from MODIS and VIIRS

NASA's Fire Information for Resource Management System (FIRMS) provides near-real time active fire / hotspot products from MODIS and VIIRS to users in over 160 countries. The goal of FIRMS is to meet the needs of natural resource and protected area managers that face considerable challenges in obtaining timely satellite-derived information on fires burning within and around their management area. FIRMS has been reliably providing active fire / hotspot data in easy to use formats since its inception in 2006. Fire information is provided through a web map interface, email alerts, a web mapping service, and a range of downloadable files (SHP, CSV, KML and JSON). FIRMS data are used directly by end users and by brokers who take the data and add value to it before re-distributing it.FIRMS was initially developed by the University of Maryland in 2006; it was funded by the United Nations FAO and NASA's Applied sciences program under a NASA ROSES call. In 2012 FIRMS became integrated in to NASA's Land Atmosphere Near real-time Capability for EOS (LANCE); a virtual system that leverages NASAs existing science processing capabilities to deliver NRT data from ten instruments within 3 hours of satellite overpass. This presentation will describe the FIRMS system, provide an overview of the system, briefly describe some of the known applications and describe plans to further integrate the data in to NASA's Global Imagery Browse Services (GIBS) public mapping services and Worldview website.

active fire/hotspots

Snowex 2017 Community Snow Depth Measurements: A Quality-Controlled, Georeferenced Product

Snow depth was one of the core ground measurements required to validate remotely-sensed data collected during SnowEx Year 1, which occurred in Colorado. The use of a single, common protocol was fundamental to produce a community reference dataset of high quality. Most of the nearly 100 Grand Mesa and Senator Beck Basin SnowEx ground crew participants contributed to this crucial dataset during 6-25 February 2017. Snow depths were measured along ~300 m transects, whose locations were determined according to a random-stratified approach using snowfall and tree-density gradients. Two-person teams used snowmobiles, skis, or snowshoes to travel to staked transect locations and to conduct measurements. Depths were measured with a 1-cm incremented probe every 3 meters along transects. In shallow areas of Grand Mesa, depth measurements were also collected with GPS snow-depth probes (a.k.a. MagnaProbes) at ~1-m intervals. During summer 2017, all reference stake positions were surveyed with <10 cm accuracy to improve overall snow depth location accuracy. During the campaign, 193 transects were measured over three weeks at Grand Mesa and 40 were collected over two weeks in Senator Beck Basin, representing more than 27,000 depth values. Each day of the campaign depth measurements were written in waterproof field books and photographed by National Snow and Ice Data Center (NSIDC) participants. The data were later transcribed and prepared for extensive quality assessment and control. Common issues such as protocol errors (e.g., survey in reverse direction), notebook image issues (e.g., halo in the center of digitized picture), and data-entry errors (sloppy writing and transcription errors) were identified and fixed on a point-by-point basis. In addition, we strove to produce a georeferenced product of fine quality, so we calculated and interpolated coordinates for every depth measurement based on surveyed stakes and the number of measurements made per transect. The product has been submitted to NSIDC in csv format. To educate data users, we present the study design and processing steps that have improved the quality and usability of this product. Also, we will address measurement and design uncertainties, which are different in open vs. forest areas.

Brucker, L.

Software Development for SonicSonde Instrumentation Suite

The SonicSonde is an innovative weather instrumentation suite for boundary layer sampling. Software development included creating a graphical user interface (GUI) to receive, process, archive, and display data in real-time. Targeted at the Windows operating system but with multi-platform extensibility in mind, the GUI was developed using Qt 5.14 in C++. A key design consideration was to allow for tailored displays and analysis while also permitting additional sensors to be added to the platform. As such, the application relies heavily on flexible interfaces and multithreading processing for capturing, modelling, and plotting data. The object-oriented nature of Qt made it ideal for this purpose. The application supports input through live serial streams and static .csv or .dat files, custom meteorological graphs, and user-dictated panel views and output intervals. Additionally, the GUI displays real-time information about the state of the instrument. The versatility of the SonicSonde will allow meteorologists and researchers to perform or better support a variety of aeronautic and atmospheric missions.

meterology

Providing Long-term Solar and Meteorological Resource Information from NASA Research through the GIS-Enabled POWER Web Services Portal

Solar and other renewable energy systems are optimized using geophysical parameters describing information about the solar resource and meteorological environments and how those environments may be changing in time. The Prediction of Worldwide Renewable Energy Resource (POWER) team at the NASA LaRC Research Center deployed its first version of the revitalized POWER geophysical parameter website that employs Esri Geographic Information System (GIS) tools. This web application provides access to both time series and climatological data sets spanning from a few days behind real-time back to the early 1980’s with a spatial resolution of 0.5 x 0.5 degree, thus extending over 30 years. The GIS tools enable, generate and store climatological averages using spatial queries and calculations in a spatial database resulting in greater accessibility to government agencies, industry and individuals. There is an API that provides data through a URL coding and can also invoked inside user software packages. Output data formats now include ASCII, CSV, geoTiff, JSON and .netCDF. Since May 2018, over 10.4 TB of data have been delivered to fulfill over 110 million data requests from 240,000 unique user IPs (https://power.larc.nasa.gov). This presentation provides an overview of this project and the current version of the new POWER web capabilities through to the end usage. Surface solar parameters are adapted from both the NASA/GEWEX (Global and Energy Water Cycle Exchange) SRB (Surface Radiation Budget) project and the CERES (Clouds and Earth’s Radiant Energy System) data products. The meteorological parameters are adapted from NASA’s MERRA-2 (Modern Era Retrospective-analysis for Research and Applications). We review uncertainty of various basic parameters using surface measurements. After the introduction, we specifically discuss various clusters of parameters for equator pointing tilted surfaces that provide for the estimation of optimal tilt angle by month and year. The discussion will emphasize a few examples of how solar and building engineers are using the data products. We will then preview the capabilities of Version 2 featuring examples of hourly solar and meteorological data products, customized user reports and expanded web mapping services from CERES data products. The web services provide a unique, expandable resource for renewable energy systems engineers to design, evaluate and optimize to environments worldwide.

Solar Resource

The DejaVu Runtime Verification Benchmark

In this paper we present a benchmark for evaluating runtime verification tools. It was originally created in order to compare the DEJAVU runtime verification tool1 with another similar tool. DEJAVU’s logic is first-order past time temporal logic. In order to monitor such properties efficiently, Binary Decision Diagrams (BDDs) [1] are used for representing the data observed in a trace. The details on the logic and its algorithm are described in e.g. [2, 3, 4]. The benchmark consists of six properties, formulated in English, and formalized in DEJAVU’s logic. For each property is provided (normally) three traces, of sizes varying from 10,000 events to one million events. Traces are represented in CSV format.

Ulus, Dogan

Effects of Energy Capture and Recovery on an N+3 Technology Level Reference Propulsion System

An initial, parametric study was performed to assess the benefits and drawbacks of waste heat recovery on a gas turbine engine. The NASA N+3 technology level reference propulsion system model, an advanced, single-aisle class, gas turbine engine was the baseline cycle. The amount of thermal energy removed from the high-energy core flow and transferred to lower-energy bypass or removed from the cycle was varied. Total pressure losses were included parametrically, as well as the effects of converting some of that thermal energy to work used within the cycle. Propulsion system thrust and thrust specific fuel consumption (TSFC) were minimally affected by total pressure losses or thermal energy removal from the core stream. However, total pressure losses in the bypass stream had significant thrust and TSFC penalties. Those penalties were partially offset if core thermal energy is transferred to the bypass stream. Core thermal energy converted to work for use within the engine cycle via a bottoming cycle showed significant thrust and TSFC benefits. In addition to these results, an engine dataset is also being made available. The dataset contains engine conditions (mass flow rate, total temperature and pressure, flow velocities, etc.) at four flight conditions (maximum climb limit, cruise, rolling takeoff and sea level static) that can be used for specific information to guide heat exchanger design to best realize these benefits with further research. The data set is a .csv (comma-separated values) file and is provided as a supplement to this report (available online from www.sti.nasa.gov).

propulsion

Benefits of using Electronic Data Sheets (EDS) with coreFlight Systems (cFS) - A Project Example

Recently there has been interest in the incorporation of core Flight Systems (cFS) with Spacecraft Onboard Interface Services (SOIS) Electronic Data Sheets (EDS) in the spaceflight software community. The Regenerative Fuel Cell project at the Glenn Research Center is using cFS architecture with EDS support for its monitoring and control software. The presentation will outline the benefits to using cFS with EDS support: First, EDS establishes a single source of truth for the definitions of data structures used throughout an entire mission that may otherwise be programmed in different languages and designed with different processor architectures. Not only does this help with inter-application communication via the software bus, but it also greatly simplifies communication between systems. An EDS Application Programming Interface (API) library allows the conversion of EDS data structures to and from native data structures. Second, bindings for other programming languages (e.g. Lua, Python, JSON) have been written to allow the creation and manipulation of EDS data objects within those languages. The RFC project uses Lua scripts to automatically generate binary configuration files at build time to be loaded into our cFS programs. We also use Python bindings in a graphical user interface (GUI) to allow an operator to send commands and view telemetry messages sent from cFS instances. Finally, using Lua scripts we can set up specific simulation scenarios to perform automatic functional testing. During the development of the RFC software, the software team put together a generic python GUI called “cFS-EDS-GroundStation” that provides a basic interface to an instance of cFS with EDS support. The GUI includes a basic telecommand and telemetry system that reads directly from the generated EDS databases. In the telecommand system, dropdown menus are populated with all user commands that are defined in EDS. In the telemetry system, telemetry messages are automatically decoded, written to the screen, and saved to a binary file. Additional Python scripts have been written to convert the binary data files into a comma separated value (CSV) format for further processing. We will demonstrate the basic use of the cFS-EDS-GroundStation software including adding additional commands and telemetry payload values in EDS and see them appear automatically in the cFS-EDS-Groundstation software. About the RFC project: The Regenerative Fuel Cell project is tasked with developing and demonstrating a power system consisting of a fuel cell and electrolyzer to provide power during a lunar day/night cycle. During the night, the fuel cell takes Hydrogen and Oxygen gasses and converts them into electricity, water, and heat. During the day, the electrolyzer takes input power (e.g. from a photovoltaic array) and converts water back into Hydrogen and Oxygen gasses.

Mathew Mccaskey

VESIcal: An Open-source Thermodynamic Model Engine for Mixed Volatile (H2O-CO2) Solubility in Silicate Melts

Modeling the solubility of volatiles in silicate melts is fundamental to the interpretation of volcanic systems and has implications for magma dynamics, eruption style, and material transport between the mantle, crust, and atmosphere. Recent advancements in computational capabilities and access to computing tools has outpaced the functionality and extensibility of previously available modeling platforms. Here we present VESIcal (Volatile Equilibria and Saturation Index calculator), the first comprehensive modeling tool for H2O, CO2, and mixed (H2O-CO2) solubility in silicate melts that: a) allows users access to seven popular models, with easy inter-comparison between models; b) provides universal functionality for all models (e.g., functions for calculating saturation pressures, degassing paths, etc.); c) can process large datasets (1,000’s of samples) automatically; d) can output computed data into an Excel spreadsheet or CSV file for post-modeling analysis; e) integrates plotting capabilities directly within the tool; and f) provides all of this within the framework of a python library, making the tool extensible by the user and allowing any of the model functions to be incorporated into any other code capable of calling python.Here we will provide a demonstration of VESIcal and its capabilities with applications to various volcanic processes affected by volatiles. VESIcal represents the first tool capable of directly comparing multiple solubility models and equations of state. We find that commonly used models predict surprisingly different volatile solubilities, particularly for pure CO2 or mixed CO2-H2O fluids. Even for melt compositions that are well represented in the calibration datasets of multiple models (e.g., MORBs), calculated solubilities for pure CO2 and pure H2O can deviate from one another by factors of >2 leading to 2x deviations in calculated saturation pressures (e.g., 5 to 10 kbar). The solubility of CO2 predicted by different rhyolitic models also differs substantially, overwhelming other sources of uncertainty such as analytical errors on measurements of volatile contents or uncertainties in crustal density profiles. This highlights the importance of model choice when drawing geological conclusions based on volatiles in magmas.

Kayla Iacovino

Microgravity Electrical Capacitance Tomography Data Set

Propellants mass gauging technologies designed to work in an accelerated environment, where the propellant remains settled at one end of the propellant tank, do not work well in a microgravity environment because the propellant is not necessarily settled. While some microgravity mass gauging technologies exist at various TRLs, most of them have major disadvantages. Improvements in microgravity propellant mass gauging will result in improvements to many areas of propellant management, which influences performance and mission assurance. Electrical Capacitance Tomography (ECT) is a sensing technology that has been used in the oil/gas industry for decades to measure multi-phase flow rate in pipes. ECT, when installed in a tank, is able to reconstruct the liquid distribution inside of the tank, which can then be integrated to obtain mass. ECT mass gauging recently achieved high accuracy in laboratory testing on the ground, and while ECT mass gauging will theoretically work during all phases of flight, it had not yet been tested in microgravity. The NASA KSC Launch Services Program, with support from the Flight Opportunities Program, successfully flew an ECT tank liquid mass gauging system experiment on a Zero-G parabolic flight aircraft. The experiment hardware was rented from a company; NASA LSP did no technology development work for this project. This STRIVES entry is for the entire raw data set, which consists of approximately 25GB of csv files of capacitance and motion data from the ground and flight tests of the experiment. The data set will be available on the NASA LSP Electronic Slosh Data Catalog (ESDC), and the public may request the data set from the authors (POC: Jed Storey, jedediah.m.nasa.gov).

mass gauging

Ramdb: The NASA Raman Spectral Database (version 1.00).

Given that, in most instances, minimal sample preparation is required and due to its contactless instrument design, Raman spectroscopy is one of the most versatile vibrational spectroscopic techniques for the chemical analysis of environmental and biological specimens. The diversity of applications of Raman spectroscopy ranges anywhere from art [1] to planetary science missions [2]. The advancement in the use of Raman spectroscopy in Solar System missions, notably in post-mission sample return analysis, requires a spectral library holding the broad range of specimens that could be found in Solar System sources. For this purpose, we have initiated the development of a Raman spectral database (Ramdb) at NASA Ames Research Center. Currently, the database includes experimental and theoretical Raman spectra of PAHs [3, 4], as well as laboratory Raman spectra of amino acids, carbon allotropes, minerals, and analogs relevance to Earth Sciences [5], Exobiology [6], Planetary [7], and Astrochemistry [8] to name just a few examples. Ramdb can be found on the web at www.astrochemistry.org/ramdb, where raw and processed Raman spectra can be downloaded in CSV format. The laboratory Raman spectra are measured using a laser Raman spectrometer (JASCO NRS-5500-532QRI). The Raman instrument is equipped with three excitation lasers, with wavelengths of 405, 532, and 785 nm. A clean silicon substrate is used as the internal standard for wavenumber calibration. Powdered samples were prepared (microscopic >10 um, grounded microscopic < 10 um) on glass slides. Some raw data exhibited a background signal arising as a combination of laser-induced fluorescence from the sample. To correct this background, we developed a Python pipeline that uses open-source Python libraries. Ramdb provides both raw and processed (using Python pipeline) data, which includes tabulated Raman shift transitions and other measurement details. The theoretical Raman band positions of PAHs (pyrene monomers and tetramer clusters) were computed using density functional theory (DFT) with the help of the Gaussian 16 suite of programs [9]. In the near future, Ramdb will serve as a repository of Raman spectral data from Laboratory Astrophysics and Planetary Science experiments involving the irradiation of organic compounds under simulated space and planetary conditions. In addition, online and offline tools will be developed for utilising the database for comparison to the user’s sample.

N Punnakayathil

Microbial Optical Data Processing: A Key Step in the Metabolic Assessment of Lunar Explorer Instrument for Space Biology Applications (LEIA) and Biosentinel’s Payload Data

The BioSensor payload platform on BioSentinel and LEIA autonomously collects optical data from microbial model organisms in liquid culture. The BioSensor is designed to monitor metabolic activity using absorbance measurements of cell density and alamarBlue, a readily available colorimetric redox indicator dye. BioSentinel, a pioneering NASA CubeSat, uses yeast to study deep space radiation. LEIA investigates radiation and lunar gravity response. The experimental setup includes 16 wells equipped with three LEDs (570, 630, and 850 nm) and their corresponding photodetectors. One well is a calibration control without biology while the rest have desiccated cultures. Autonomous rehydration initiates the experiment. Data from the BioSensor are received from the flight and ground units, enabling comparison to uncover location-based metabolic rate variations. This study presents a Python Jupyter notebook developed for efficient data processing of multiple CSV files containing date and time columns, temperature, and well illumination data. It offers a user-friendly interface while maintaining computational power, automatically recognizing and iteratively processing data files in a user-input path. A Hampel filter with a short window eliminates outlier artifacts from sensor dropout. Because absorbance is a relative measurement, conversion from raw illumination requires defining a “blank” value, so the first data points are averaged to provide the necessary denominator. A cube-root function correction mitigates undesired drift caused by air pockets during the fluidic card filling phase, maintaining optical path length consistency. Beer-Lambert's law is applied to further convert absorbance values to cell and dye form concentrations, the desired science parameters. The processed data are saved and visualized as SVG plots. Future plans include extracting specific science parameters from the processed data like growth rate and metabolic rate, and identification of features corresponding to metabolic and phenotypic shifts such as starvation, shifts from aerobic to anaerobic growth, and osmotic stresses.

Space biology