Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “BEEF”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 37 records · Page 2
All sourcesNASA NTRSDOE OSTI
NASA NTRS1992

Where's the Beaverhead beef?

Only rare quartz grains with single-set planar (1013) deformation features (PDF's) are present in breccia dikes found in association with uniformly oriented shatter cones that occur over an area 8 x 25 km. This suggests that the Beaverhead shocked rocks come from only the outer part of the central uplift of what must have been a large (greater than 100 km diameter) complex impact structure. An impact event of this magnitude on continental crust (thought to have occurred in late Precambrian or ealy Paleozoic time) could be expected to punctuate local geologic history. Furthermore, although it may now be covered, its scar should remain despite all the considerable subsequent erosion/deposition and tectonism since the impact. The following are three large-scale singularities or anomalies that may reflect the event and mark its source. (1) The Lemhi Arch is a major structural uplift that occurred in late Proterozoic-early Paleozoic time in East Central Idaho and caused the erosion of at least 4 km of sedimentary cover. This may be directly related to the impact. (2) Of the many thrust sheets comprising the Cordilleran belt, the Cabin plate that carries the shocked rocks is unique in that it alone intersected the crystalline basement. It also now marks the apex of the Southwest Montana Recess in the Sevier front. The basement uplift remaining from the impact may have constituted a mechanical obstacle to the advancing thrust sheets in Cretaceous time, causing the recess. (3) What could be interpreted as a roughly circular aeromagnetic anomaly approx. 70 km in diameter can be discerned in the state aeromagnetic map centered about 20 km southeast of Challis, Idaho, in the Lost River range. It is in approximately the right place, and ignoring the possibility that the anomalies have diverse causes and the circular pattern is coincidental, it may mark what remains of the buried central uplift structure.

Hargraves, R. B.↗
NASA NTRS1992

Here's the beef: A case study in organizational transformation

The Science and Technology Lab (STL) is tasked with the design, development, and application of the science and engineering services. Formed in the early 1970's STL adhered to many traditional attitudes including barriers to communication, excessive management control, parochial strategies, unclear measures of success, lack of customer focus, underutilization of people, and excessive administrative burdens on scientists and engineers. The challenge for the STL was to maximize customer satisfaction through the effective and efficient application of the notable skills and talents of the STL's workforce. In this way, the Lab would begin its exciting journey toward becoming world class. A discussion of this on-going transformation is presented.

Huseonica, William F.↗
NASA NTRS1994

Distributed parameter modelling of flexible spacecraft: Where's the beef?

This presentation discusses various misgivings concerning the directions and productivity of Distributed Parameter System (DPS) theory as applied to spacecraft vibration control. We try to show the need for greater cross-fertilization between DPS theorists and spacecraft control designers. We recommend a shift in research directions toward exploration of asymptotic frequency response characteristics of critical importance to control designers.

Hyland, D. C.↗
DOE OSTI2016

Membrane insertion for the detection of lipopolysaccharides: Exploring the dynamics of amphiphile-in-lipid assays

Shiga toxin-producing Escherichia coli is an important cause of foodborne illness, with cases attributable to beef, fresh produce and other sources. Many serotypes of the pathogen cause disease, and differentiating one serotype from another requires specific identification of the O antigen located on the lipopolysaccharide (LPS) molecule. The amphiphilic structure of LPS poses a challenge when using classical detection methods, which do not take into account its lipoglycan biochemistry. Typically, detection of LPS requires heat or chemical treatment of samples and relies on bioactivity assays for the conserved lipid A portion of the molecule. Our goal was to develop assays to facilitate the direct and discriminative detection of the entire LPS molecule and its O antigen in complex matrices using minimal sample processing. To perform serogroup identification of LPS, we used a method called membrane insertion on a waveguide biosensor, and tested three serogroups of LPS. The membrane insertion technique allows for the hydrophobic association of LPS with a lipid bilayer, where the exposed O antigen can be targeted for specific detection. Samples of beef lysate were spiked with LPS to perform O antigen specific detection of LPS from E. coli O157. To validate assay performance, we evaluated the biophysical interactions of LPS with lipid bilayers both in- and outside of a flow cell using fluorescence microscopy and fluorescently doped lipids. Our results indicate that membrane insertion allows for the qualitative and reliable identification of amphiphilic LPS in complex samples like beef homogenates. In addition, we also demonstrated that LPS-induced hole formation does not occur under the conditions of the membrane insertion assays. Together, these findings describe for the first time the serogroup-specific detection of amphiphilic LPS in complex samples using a membrane insertion assay, and highlight the importance of LPS molecular conformations in detection architectures.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2020

Integrated energy-water-land nexus planning to guide national policy: an example from Uruguay

Despite broad consensus on the benefits of a nexus approach to multi-sector planning, actual implementation in government and other decision-making institutions is still rare. This study presents an approach to conducting integrated energy-water-land (EWL) planning, using Uruguay as an example. This stakeholder-driven study focuses on assessing the EWL nexus implications of actual planned policies aimed at strengthening three of Uruguay's key exports (beef, soy, and rice), which account for more than 40% of total national export revenue. Five scenarios are analyzed in the study: a reference scenario, a climate impacts scenario, and three policy scenarios. The three policy scenarios include measures such as increasing the intensity of beef production while simultaneously decreasing emissions, increasing irrigated soybean production, and improving rice yields. This study supplements previous sector-specific planning efforts in Uruguay by conducting the first stakeholder-driven integrated multi-sector assessment of planned policies in Uruguay using a suite of integrated modeling tools. Key insights from the study are: as compared to a reference scenario, improving beef productivity could lead to cropland expansion (+30%) and significant indirect increases in water requirements (+20%); improving rice yields could lead to increases in total emissions (+3%), which may partially offset emissions reductions from other policies; expanding irrigated soy could have the least EWL impacts amongst the policies studied; and climate-driven changes could have significantly less impact on EWL systems as compared to human actions. The generalizable insights derived from this analysis are readily applicable to other countries facing similar multi-sector planning challenges. In particular, the study's results reinforce the fact that policies often have multi-sector consequences, and thus policies can impact one another's efficacy. Thus, policy design and implementation can benefit from coordination across sectors and decision-making institutions.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1999

Sensitive detection of Escherichia coli O157:H7 in food and water by immunomagnetic separation and solid-phase laser cytometry

Rapid, direct methods are needed to assess active bacterial populations in water and foods. Our objective was to determine the efficiency of bacterial detection by immunomagnetic separation (IMS) and the compatibility of IMS with cyanoditolyl tetrazolium chloride (CTC) incubation to determine respiratory activity, using the pathogen Escherichia coli O157:H7. Counterstaining with a specific fluorescein-conjugated anti-O157 antibody (FAb) following CTC incubation was used to allow confirmation and visualization of bacteria by epifluorescence microscopy. Broth-grown E. coli O157:H7 was used to inoculate fresh ground beef (<17% fat), sterile 0.1% peptone, or water. Inoculated meat was diluted and homogenized in a stomacher and then incubated with paramagnetic beads coated with anti-O157 specific antibody. After IMS, cells with magnetic beads attached were stained with CTC and then an anti-O157 antibody-fluorescein isothiocyanate conjugate and filtered for microscopic enumeration or solid-phase laser cytometry. Enumeration by laser scanning permitted detection of ca. 10 CFU/g of ground beef or <10 CFU/ml of liquid sample. With inoculated meat, the regression results for log-transformed respiring FAb-positive counts of cells recovered on beads versus sorbitol-negative plate counts in the inoculum were as follows: intercept = 1.06, slope = 0.89, and r2 = 0. 95 (n = 13). The corresponding results for inoculated peptone were as follows: intercept = 0.67, slope = 0.88, and r2 = 0.98 (n = 24). Recovery of target bacteria on beads by the IMS-CTC-FAb method, compared with recovery by sorbitol MacConkey agar plating, yielded greater numbers (beef, 6.0 times; peptone, 3.0 times; water, 2.4 times). Thus, within 5 to 7 h, the IMS-CTC-FAb method detected greater numbers of E. coli O157 cells than were detected by plating. The results show that the IMS-CTC-FAb technique with enumeration by either fluorescence microscopy or solid-phase laser scanning cytometry gave results that compared favorably with plating following IMS.

Non-NASA Center↗
DOE OSTI2026

Interactive effects of precipitation, temperature, and grazing pressure on the net ecosystem carbon balance of grazing lands across the continental United States

Grazing lands cover approximately one-third of the contiguous United States, support much of the nation's beef production, and are an important component of the U.S. terrestrial carbon budget. In this study, we quantified net ecosystem carbon balance (NECB), the net status of grazing lands as a carbon sink (C-sink) or source (C-source) and a key determinant of soil health and productivity. Our primary objective was to synthesize multiple years of annual NECB across heterogeneous grazing lands across the continental U.S and evaluate annual NECB against physical drivers (mean annual precipitation (MAP), mean annual temperature (MAT), vegetation, and moisture condition) and management practices (grazing pressure index (GPI) and fertilization history). We hypothesized that (1) NECB is higher in mesic and fertilized grasslands; (2) NECB increases with MAP and MAT but decreases with GPI; and (3) interactive effects exist among MAP, MAT, and GPI. Using carbon fluxes measured by eddy covariance towers and methane emissions including both enteric methane and manure derived from stocking rates across seven USDA Long-term Agroecosystem Research Network sites, we found: (1) grazing lands were a C-sink or carbon neutral at most sites; (2) vegetation type, moisture conditions, or fertilization had no significant effect on NECB; (3) NECB increased with MAP and MAT, but decreased with a higher GPI; and (4) MAT had a significant positive effect on NECB when MAP exceeded 750 mm (greater water availability). The effect of GPI on NECB was significantly negative when MAP was below 1000 mm, significantly negative when MAT < 12 °C and significantly positive when MAT > 16 °C. Thus, most grazing lands in our study acted as C-sinks unless water deficit, low temperature, or heavy grazing were interactively present. Understanding how climate and management influence NECB of grazing lands is key to maintaining resilient agroecosystems that secure beef production and sustain rural prosperity.

LTAR↗
DOE OSTI2015

Purification and characterization of lipopolysaccharides from six strains of non-O157 Shiga toxin-producing Escherichia coli

Certain Shiga toxin-producing Escherichia coli (STEC) are virulent human pathogens that are most often acquired through contaminated food. The United States Department of Agriculture, Food Safety and Inspection Service has declared several serogroups of STEC as adulterants in non-intact raw beef products. Hence, sensitive and specific tests for the detection of these STEC are a necessity for implementation in food safety programs. E. coli serogroups are identified by their respective O-antigen moiety on the lipopolysaccharide (LPS) macromolecule. We propose that the development of O-antigen-specific immunological assays can facilitate simple and rapid discriminatory detection of STEC in beef. However, the resources (antigens and antibodies) required for such development are not readily available. To overcome this, we extracted and characterized LPS and O-antigen from six STEC strains. Using hot phenol extraction, we isolated the LPS component from each strain and purified it using a series of steps to eliminate proteins, nucleic acids, and lipid A antigens. Antigens and crude LPS extracts were characterized using gel electrophoresis, immunoblotting, and modified Western blotting with commercially available antibodies, thus assessing the serogroup specificity and sensitivity of available ligands as well. The results indicate that, while many commercially available antibodies bind LPS, their activities and specificities are highly variable, and often not as specific as those required for serogroup discrimination. This variability could be minimized by the production of antibodies specific for the O-antigen. Additionally, the antigens generated from this study provide a source of characterized LPS and O-antigen standards for six serogroups of STEC.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2022

A comparative analysis of different van der Waals treatments for molecular adsorption on the basal plane of 2H-MoS 2

The binding energy of hydrogen sulfide, ammonia, ethane, ethylene, butadiene, benzene, toluene, pyridine, pyrrole, and thiophene on the basal plane of the semi-conducting 2H-molybdenum sulfide (MoS 2 ) was calculated with the following flavors of Density Functional Theory (DFT): GGA-PW91, PBE-D3, vdW-DF, optPBE, optB86b, optB88, vdW-TS, and BEEF-vdW. The GGA-PW91 binding energies are negligible (<0.07 eV in magnitude) in all cases. The predictions with vdW-DF and PBE-D3 are the closest (error <0.05 eV) to the isosteric heats of adsorption calculated from reported temperature programmed desorption data for thiophene and butadiene. For all dispersion flavors examined here, the magnitude of the dispersion contribution to the binding energy increases linearly with the number of heavy atoms in the adsorbate, with each atom contributing 0.05 eV (BEEF-vdW) – 0.09 eV (optB88-vdW). Further, this implies that the calculated adsorption constants of molecules larger than acridine (i.e., comprising > 14 non-heavy atoms) can vary by more than four orders of magnitude at industrial conditions depending on the chosen method of dispersion correction. Further, dispersion effects fall off rapidly (>0.03 eV/ non-hydrogen atom/Å) as the adsorbate-surface distance increases.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗